KR20200006819A - Blood sugar bio sensor - Google Patents

Abstract

Disclosed is a blood sugar biosensor. The blood sugar biosensor comprises: an electrode layer arranged in a first region of a paper film; a protective film layer printed with a hydrophobic material on a second region of the paper film not the first region and the electrode layer and having a predetermined opened region into which blood is injected; and an enzyme layer arranged in the predetermined opened region and the electrode layer and printed with an enzyme responding to the blood. Therefore, the blood sugar biosensor can be simply and quickly manufactured.

Description

Blood glucose biosensor {BLOOD SUGAR BIO SENSOR}

The present invention relates to a blood glucose biosensor, and more particularly, to a blood glucose biosensor implemented on a paper film using a printing technique.

In modern times, the number of diabetic patients is increasing rapidly every year due to the environmental factors such as high calorie, high fat, high protein diet, lack of exercise, and stress due to westernization of the diet. It is important.

On the other hand, the biosensor is a device for analysis combining a biological element and a physicochemical detector, it can be used to search for analytes. One of the most common commercial biosensors is the blood glucose biosensor.

Existing electrochemical blood glucose biosensor has a three-layer structure consisting of a lower plate and the upper plate to form a channel, the upper plate and the lower plate to bond the electrode is printed. The fabrication process is complicated because each layer must be fabricated individually and adhered with precise alignment. In fact, due to the problem of reproducibility of the manufacturing process, the output characteristic difference of the blood glucose sensor strip may occur somewhat, and the manufacturing process must be complicated to solve this problem.

Therefore, there is a need for a blood glucose biosensor that can measure blood glucose accurately while the manufacturing process is simple.

Existing electrochemical blood glucose biosensors are mostly made of plastic film, which causes environmental pollution. As water and drinking water pollution caused by fine plastic particles has been raised, the movement to restrict the use of household plastics is rapidly progressing around Europe.

Therefore, there is a need for an environmentally friendly blood glucose biosensor that does not use plastic films.

On the other hand, the above information is only presented as background information to help the understanding of the present invention. No determination is made as to whether any of the above is applicable as the prior art concerning the present invention, and no claims are made.

Patent Registration No. 10-1171457 (Registration Date: 2012.7.31)

The present invention has been made to solve the above-described problem, an embodiment of the present invention proposes a blood glucose biosensor that is implemented on a paper film in detail using a printing technique.

Technical problems to be achieved in the present invention are not limited to the above-mentioned technical problems, and other technical problems not mentioned above will be clearly understood by those skilled in the art from the following description. Could be.

Blood glucose biosensor according to an embodiment of the present invention for realizing the above object is an electrode layer disposed in the first region of the paper film; A protective film layer printed with a hydrophobic material on the second region of the paper film and the electrode layer instead of the first region, and having a predetermined region into which blood is injected; And an enzyme layer disposed in the opened predetermined region and on the electrode layer, and the enzyme reacting with the blood is printed.

More specifically, further comprising an enzyme protective cover layer for protecting the enzyme layer, wherein the enzyme protective cover layer is a region where a predetermined end of the paper film is extended, the extended region is the paper around a specific line Can be stacked on film.

Blood glucose biosensor according to another embodiment of the present invention for realizing the above object is an electrode layer disposed in the first region of one paper film; A passivation layer printed with a hydrophobic material and formed so as to open a region into which blood is injected, and directly adjacent the first region; An enzyme layer disposed in the opened region and on the electrode layer, and printed with an enzyme that reacts with the blood; And a protective layer printed on a portion of the protective layer, and when the protective layer is superimposed on the first region around a specific line, the adhesive layer adheres the protective layer and the first region.

According to another embodiment of the present invention for realizing the above object, the blood glucose biosensor includes: an electrode layer on which electrodes are printed on a plurality of first regions included in one paper film; A passivation layer corresponding to each of the plurality of first regions and having a hydrophobic material printed on a predetermined region of each of the plurality of second regions arranged to overlap one-to-one with each of the plurality of first regions; An enzyme layer printed on a specific terminal region of each of the plurality of electrode layers; And an adhesive layer printed on a portion of each of the plurality of second regions, the adhesive layer for bonding each of the first regions corresponding to each of the plurality of second regions, wherein the plurality of passivation layers include the enzyme layer. The opened area may be provided in the designated area.

According to another embodiment of the present invention for realizing the above object, the blood glucose biosensor includes an electrode layer disposed in a first region of one paper film; An enzyme layer printed on the electrode layer and reacting with blood; A second region adjacent to one end of the first region and including a blood inlet, wherein the second region is formed so that the enzyme layer is opened when overlapped with the first region; And a third region adjacent to the other end of the first region and including a vent, wherein the second region overlaps the first region about a first specific line, and the third region forms a second specific line. It can be superimposed with the first region and the third region as a center.

According to various embodiments of the present invention, the following effects may be derived.

First, since the blood glucose biosensor may be manufactured by several printing processes, the blood glucose biosensor may be manufactured simply and the sensor manufacturing may be performed quickly.

Second, since a plurality of blood glucose biosensors can be manufactured at the same time by a simple printing process, the blood sugar biosensors can be manufactured inexpensively and quickly.

Third, it is eco-friendly because paper film is used instead of the plastic film generally used for blood glucose biosensors.

Effects obtained in the present invention are not limited to the above-mentioned effects, and other effects not mentioned above may be clearly understood by those skilled in the art from the following description. will be.

1 (a) to 1 (d) is a view for schematically explaining a process of manufacturing a blood glucose biosensor according to an embodiment of the present invention.
2 (a) to 2 (e) is a view for explaining the manufacturing process of the blood glucose biosensor according to an embodiment of the present invention.
3 is a cross-sectional view of a blood glucose biosensor according to an embodiment of the present invention.
4 to 9 are diagrams for describing a blood glucose biosensor according to various embodiments of the present disclosure.
As described above, the present invention has been illustrated and described with reference to preferred embodiments, but is not limited to the above-described embodiments, and is provided to those skilled in the art without departing from the spirit of the present invention. Various changes and modifications will be possible.

Hereinafter, various embodiments of the present disclosure will be described in detail with reference to the accompanying drawings. However, in describing the present invention, when it is determined that a detailed description of related known functions or configurations may unnecessarily obscure the subject matter of the present invention, a detailed description thereof will be omitted.

1 (a) to 1 (d) are diagrams for schematically explaining a process of manufacturing a blood glucose biosensor (blsua) 100 according to an embodiment of the present invention. It can be summarized into three processes.

First, an electrode layer, a protective layer, and an enzyme layer constituting a blood glucose biosensor may be designed through a personal computer (PC). For example, the plurality of layers constituting the biosensor may be designed through a computer aided design (CAD) program (FIG. 1A). The design can be automated and performed even without user intervention.

Next, an electrode layer, a protective layer and an enzyme layer designed through a printer can be printed (Figs. 1 (b) and 1 (c)). The printing may be applied to inkjet printing technology. A material necessary for printing the electrode layer, the protective layer and the enzyme layer may be included in the ink.

Finally, the printed plurality of layers is dried, and the blood glucose biosensor blsua 100 can be completed by a cutting process (FIG. 1 (d)).

Hereinafter, the blood glucose biosensor 100 according to an embodiment of the present invention will be described. 2 (a) to 2 (e) is a view for explaining the manufacturing process of the blood glucose biosensor 100 according to an embodiment of the present invention, Figure 3 is a blood sugar biosensor according to an embodiment of the present invention 100 is a cross-sectional view.

The paper film 110 illustrated in FIGS. 2A to 3 may be a film made of paper or paper. However, in the implementation, the paper film 110 may be replaced with a flat plastic film. Paper film 110 may be implemented in the size of the blood glucose biosensor 100 from the initial process, prepared in the size of a plurality of blood glucose biosensors may be produced in the size of the blood glucose biosensor through a cutting process.

Referring to FIG. 2B, electrode layers e and 120 may be disposed in a predetermined region on the paper film 110. The electrode layer 120 may be disposed in a portion of the paper film 110, and the electrode layer 120 may include a working electrode 120A and a reference electrode 120B. Here, the reference electrode 120B is a necessary electrode because the absolute value thereof is not measured when measuring the electrode potential generated between the solution and the metal.

The electrode layer 120 may be manufactured by a printing process using conductive ink. Inkjet printing technology may be used in the printing process, and screen printing technology and dispensing technology may be used depending on the embodiment.

When printing is performed, the printer may be automatically driven, and when printing the electrode layer 120, a material for printing the electrode layer 120 is automatically output to the printing outlet to be printed on the paper film 110. Can be.

In addition, the electrode layer 120 is measured in addition to the working electrode 120A and the reference electrode 120B, a strip recognition electrode and blood for determining that the blood glucose biosensor strip is inserted into the measurement system (main body) in a state capable of being measured. It may further include a blood recognition electrode, etc. for determining whether it is applied in a possible state, and in implementation, more various electrodes may be printed on the electrode layer 120.

Referring to FIG. 2 (c), a protective film layer disposed on the electrode layer 120, in which a hydrophobic material is printed on a predetermined region of the paper film 110, and a predetermined region into which blood is injected is opened (punched). 130 may be disposed. A portion of the passivation layer 130 may be disposed on the electrode layer 120, and another portion may be disposed on the paper film 110. The passivation layer 130 may be printed with hydrophobic ink to serve as an insulating layer.

In addition, the protective film layer 130 has one purpose for protecting the electrode, and the protective film layer 130 has another purpose for applying blood only to the electrode layer 120 to which the enzyme layer 140 (to be described later) is printed. .

Referring to FIG. 2 (d), the enzyme layer 140 may be disposed inside and on the electrode layer 120 of an open area of the passivation layer 130, and is a layer on which an enzyme reacting with blood is printed. The enzyme may be a glucose enzyme, but may be implemented in various materials.

The printer which performs the process of FIGS. 2 (a) to 2 (d) may automatically perform the processes according to the process.

FIG. 2 (e) is a sectional view of FIG. 2 (d) in the X to X ″ direction. The blood glucose biosensor 100 includes a paper film 110, an electrode layer 120, a passivation layer 130, and an enzyme layer 140.

Referring to FIG. 3, blood blo may react with the enzyme layer, and blood glucose may be measured according to a reaction such as oxidation at an electrode of the electrode layer 120. Blood glucose biosensor 100 according to an embodiment of the present invention is a sensor is applied to the upper blood applying method is applied by directly contacting the blood on the electrode, a method of injecting blood using a capillary phenomenon through the existing narrow channel User convenience can be improved.

4 to 9 are diagrams for describing a blood glucose biosensor according to various embodiments of the present disclosure. Hereinafter, different blood glucose biosensors will be described with reference to the drawings.

First, Figure 4 shows a blood glucose biosensor 100 according to an embodiment of the present invention.

The blood glucose biosensor 100 disclosed in FIG. 4 may further include an enzyme protective cover hou 150. Enzyme protective cover 150 is a cover for protecting the enzyme layer 140, the region extending a predetermined end of the paper film 110 may be superimposed on the paper film 110 around a specific line (fol). . Accordingly, the enzyme layer 140 is protected to prevent the enzyme layer from being contaminated or lost when the blood glucose biosensor is handled.

Next, Figure 5 shows a blood glucose biosensor 100 according to an embodiment of the present invention.

The blood glucose biosensor 100 may be manufactured in a folded form using a single sheet of paper film. The first region on which the electrode layer 120 is disposed and the second region on which the protective layer 130 is printed may be printed on one paper film. The first region and the second region may be directly adjacent to each other.

The passivation layer 130 may be printed with a hydrophobic material to prevent the introduction of the hydrophilic material, and may be formed to be opened (punched) in the case of the regions (win, 170) into which blood is injected.

The enzyme layer 140 is disposed in the opened region and on the electrode layer 120, and is a layer on which an enzyme that reacts with blood is printed.

In addition, an adhesive layer 180 is printed on a part of the protective layer 130, and the adhesive layer 180 is formed when the second region including the protective layer 130 overlaps the first region and a specific line. The first region and the second region may be bonded. Here, the adhesive layer 180 may also be arranged using a printing technique, and various adhesive materials may be applied.

6 and 7 illustrate fabricating a plurality of blood glucose biosensors simultaneously in an assembly manner according to an embodiment of the present invention.

Blood glucose biosensors can be made simultaneously from one paper film. To this end, an electrode layer may be printed on a plurality of first regions included in one paper film 15. A passivation layer on which a hydrophobic material is printed may be disposed in second areas that correspond to each of the first areas in which the electrode layers are printed, respectively. In addition, an enzyme layer may be disposed in each of the first regions. After printing, a plurality of blood glucose biosensors may be manufactured by origami.

An adhesive layer 180A to 180D for bonding each of the plurality of second regions and each of the first regions corresponding to each of the plurality of second regions may be printed on a portion of each of the plurality of second regions, and the plurality of openings may be printed. Regions 170A through 170E may also be disposed. The plurality of opening regions correspond to regions for opening the enzyme layer as regions where blood reacts.

8 and 9 illustrate a blood glucose biosensor 100 according to an embodiment of the present invention. The blood sugar biosensor 100 described herein corresponds to the blood sugar biosensor 100 using a capillary phenomenon. 8 illustrates manufacturing one blood glucose biosensor and FIG. 9 illustrates manufacturing a plurality of blood glucose biosensors.

First, referring to FIG. 8, one film may be divided into first to third regions, and an electrode layer and an enzyme layer on a portion of the electrode layer may be disposed in the first region. The second region may be overlapped with the first region, and may include an opening through which the enzyme layer is opened. The third region may include an air vent adjacent to the other end of the first region.

The opening in which the enzyme layer is opened may be represented by a channel, and the vent may be represented by a pore.

First, the second region may overlap the first region around the first specific line, and the third region may overlap the first region and the third region around the second specific line.

9 illustrates a method of simultaneously manufacturing a blood glucose biosensor using a capillary phenomenon using one film. According to FIG. 9, a total of eight blood glucose biosensors may be manufactured at the same time.

On the other hand, this specification is not intended to limit the invention to the specific terms presented. Thus, while the present invention has been described in detail with reference to the examples described above, those skilled in the art can make modifications, changes and variations to the examples without departing from the scope of the invention. The scope of the present invention is shown by the following claims rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention. do.

Claims (5)

An electrode layer disposed in the first region of the paper film;
A protective film layer printed with a hydrophobic material on the second region of the paper film and the electrode layer instead of the first region, and having a predetermined region into which blood is injected; And
A blood glucose biosensor, disposed within the predetermined region opened and on the electrode layer, includes an enzyme layer printed with an enzyme that reacts with the blood. The method of claim 1,
Further comprising an enzyme protective cover layer for protecting the enzyme layer,
The enzyme protective cover layer,
The blood glucose biosensor, wherein a predetermined end of the paper film is an extended area, and the extended area is superimposed on the paper film about a specific line. An electrode layer disposed in the first region of one paper film;
A passivation layer printed with a hydrophobic material and formed so as to open a region into which blood is injected, and directly adjacent the first region;
An enzyme layer disposed in the opened region and on the electrode layer, and printed with an enzyme that reacts with the blood; And
A blood glucose biosensor, which is printed on a part of the passivation layer, and includes the adhesive layer adhering the passivation layer and the first area when the passivation layer is superimposed on the first region around a specific line. An electrode layer on which electrodes are printed on a plurality of first regions included in one paper film;
Correspond to each of the plurality of first regions,
A passivation layer on which a hydrophobic material is printed on a predetermined region of each of the plurality of second regions arranged to overlap one-to-one with each of the plurality of first regions;
An enzyme layer printed on a specific terminal region of each of the plurality of electrode layers; And
And an adhesive layer printed on a portion of each of the plurality of second regions, for bonding each of the first regions corresponding to each of the plurality of second regions.
The plurality of protective film layers are provided with a region opened in the region where the enzyme layer is arranged, blood glucose biosensor. An electrode layer disposed in the first region of one paper film;
An enzyme layer printed on the electrode layer and reacting with blood;
A second region adjacent to one end of the first region and including a blood inlet, wherein the second region is formed so that the enzyme layer is opened when overlapped with the first region; And
And a third region adjacent the other end of the first region and including a vent.
And the second region overlaps the first region about the first specific line, and the third region overlaps the first region and the third region about the second specific line.

Images