JP2009063300A - 基質の結合と反応生成物を同時に検出できるバイオセンサー - Google Patents
基質の結合と反応生成物を同時に検出できるバイオセンサー Download PDFInfo
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- JP2009063300A JP2009063300A JP2007228689A JP2007228689A JP2009063300A JP 2009063300 A JP2009063300 A JP 2009063300A JP 2007228689 A JP2007228689 A JP 2007228689A JP 2007228689 A JP2007228689 A JP 2007228689A JP 2009063300 A JP2009063300 A JP 2009063300A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G—PHYSICS
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- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
- G01N21/774—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides the reagent being on a grating or periodic structure
- G01N21/7743—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides the reagent being on a grating or periodic structure the reagent-coated grating coupling light in or out of the waveguide
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
【解決手段】生理活性物質に特異的に結合する被験分子を検出するバイオセンサーであって、前記生理活性物質が固定化されている第一の反応部と、前記生理活性物質による生理活性反応によって生じた反応生成物に特異的に結合する分子が固定化されている第二の反応部とを同一エリア内に含み、かつ、前記第一の反応部における結合反応と、前記第二の反応部における結合反応を求める測定部を有することを特徴とするバイオセンサー。
【選択図】なし
Description
(1)(a)前記生理活性物質が固定化されている第一の反応部であって、前記生理活性物質と前記被験分子との結合反応と、前記生理活性物質による生理活性反応とを行うための第一の反応部と、(b)前記生理活性反応によって生じた反応生成物に特異的に結合する分子が固定化されている第二の反応部であって、前記反応生成物と、前記反応生成物に特異的に結合する分子との結合反応を行うための第二の反応部とを同一エリア内に含み、かつ、
(2)前記第一の反応部における結合反応と、前記第二の反応部における結合反応を求める測定部を有することを特徴とするバイオセンサーが提供される。
好ましくは、本発明のバイオセンサーは、前記第二の反応部を二つ以上保持している。
好ましくは、前記第一の反応部と前記第二の反応部とがエアギャップにより遮断されている。
好ましくは、前記第一の反応部における結合反応と前記第二の反応部における結合反応の変化は、誘電率の変化である。
好ましくは、前記測定部は導波路である。
本発明のバイオセンサーは、生理活性物質に特異的に結合する被験分子を検出するバイオセンサーであって、
(1)(a)前記生理活性物質が固定化されている第一の反応部であって、前記生理活性物質と前記被験分子との結合反応と、前記生理活性物質による生理活性反応とを行うための第一の反応部と、(b)前記生理活性反応によって生じた反応生成物に特異的に結合する分子が固定化されている第二の反応部であって、前記反応生成物と、前記反応生成物に特異的に結合する分子との結合反応を行うための第二の反応部とを同一エリア内に含み、かつ、
(2)前記第一の反応部における結合反応と、前記第二の反応部における結合反応との変化を求める測定部を有することを特徴とするバイオセンサーである。
核酸としては、測定の対象とする核酸と相補的にハイブリダイズするものを使用することができる。核酸は、DNA(cDNAを含む)、RNAのいずれも使用できる。DNAの種類は特に限定されず、天然由来のDNA、遺伝子組換え技術により調製した組換えDNA、又は化学合成DNAの何れでもよい。
低分子有機化合物としては通常の有機化学合成の方法で合成することができる任意の化合物が挙げられる。
免疫グロブリン結合性蛋白質としては、例えばプロテインAあるいはプロテインG、リウマチ因子(RF)等を使用することができる。
糖結合性蛋白質としては、レクチン等が挙げられる。
脂肪酸あるいは脂肪酸エステルとしては、ステアリン酸、アラキジン酸、ベヘン酸、ステアリン酸エチル、アラキジン酸エチル、ベヘン酸エチル等が挙げられる。
金薄膜基板2をModel-208UV−オゾンクリーニングシステム(TECHNOVISION INC.)で30分間処理した後、エタノール/水(80/20)中11−ヒドロキシ-1-ウンデカンチオールの5.0mM溶液を金属膜に接触するように添加し、25℃で18時間表面処理を行った。その後、エタノールで5回、エタノール/水混合溶媒で1回、水で5回洗浄を行った。
次に、11−ヒドロキシ-1-ウンデカンチオールで被覆した表面を10質量%のエピクロロヒドリン溶液(溶媒:0.4M水酸化ナトリウム及びジエチレングリコールジメチルエーテルの1:1混合溶液)に接触させ、25℃の振盪インキュベーター中で4時間反応を進行させた。表面をエタノールで2回、水で5回洗浄した。
次に、25質量%のデキストラン(T500,Pharmacia)水溶液40.5mlに4.5mlの1M水酸化ナトリウムを添加し、その溶液をエピクロロヒドリン処理表面上に接触させた。次に振盪インキュベーター中で25℃で20時間インキュベートした。表面を50℃の水で10回洗浄した。
続いて、ブロモ酢酸3.5gを27gの2M水酸化ナトリウム溶液に溶解した混合物を上記デキストラン処理表面に接触させて、28℃の振盪インキュベーターで16時間インキュベートした。表面を水で洗浄し、その後上述の手順を1回繰り返して、親水性膜1を作製した。
2 金薄膜基板
3 プリズム
4 反応部
5 酵素
6 基質
7 反応生成物
8 特異的結合分子
9 測定装置
10 固定部
11 固定部
12 固定部
13 酵素
14 基質
15 反応生成物
Claims (7)
- 生理活性物質に特異的に結合する被験分子を検出するバイオセンサーであって、
(1)(a)前記生理活性物質が固定化されている第一の反応部であって、前記生理活性物質と前記被験分子との結合反応と、前記生理活性物質による生理活性反応とを行うための第一の反応部と、(b)前記生理活性反応によって生じた反応生成物に特異的に結合する分子が固定化されている第二の反応部であって、前記反応生成物と、前記反応生成物に特異的に結合する分子との結合反応を行うための第二の反応部とを同一エリア内に含み、かつ、
(2)前記第一の反応部における結合反応と、前記第二の反応部における結合反応を求める測定部を有することを特徴とするバイオセンサー。 - 前記第一の反応部を二つ以上保持している、請求項1に記載のバイオセンサー。
- 前記第二の反応部を二つ以上保持している、請求項1又は2に記載のバイオセンサー。
- 前記第一の反応部と前記第二の反応部とがエアギャップにより遮断されている、請求項1から3の何れかに記載のバイオセンサー。
- 前記エアギャップが移動可能なエアギャップである、請求項4に記載のバイオセンサー。
- 前記第一の反応部における結合反応と前記第二の反応部における結合反応の変化が、誘電率の変化である、請求項1から5の何れかに記載のバイオセンサー。
- 前記測定部が導波路である、請求項1から6の何れかに記載のバイオセンサー。
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EP20080015545 EP2034295A1 (en) | 2007-09-04 | 2008-09-03 | Biosensor capable of simultaneous detection of substrate binding and reaction product |
US12/203,676 US7993912B2 (en) | 2007-09-04 | 2008-09-03 | Biosensor capable of simultaneous detection of substrate binding and reaction product |
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JP5255247B2 (ja) | 2013-08-07 |
US7993912B2 (en) | 2011-08-09 |
EP2034295A1 (en) | 2009-03-11 |
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