JP2009028011A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2009028011A5 JP2009028011A5 JP2007197754A JP2007197754A JP2009028011A5 JP 2009028011 A5 JP2009028011 A5 JP 2009028011A5 JP 2007197754 A JP2007197754 A JP 2007197754A JP 2007197754 A JP2007197754 A JP 2007197754A JP 2009028011 A5 JP2009028011 A5 JP 2009028011A5
- Authority
- JP
- Japan
- Prior art keywords
- strain
- bacillus subtilis
- ctf
- experimental example
- red fluorescent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000276408 Bacillus subtilis subsp. subtilis str. 168 Species 0.000 description 3
- 230000002688 persistence Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 230000000241 respiratory Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000035812 respiration Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
Description
以上のように、本実験例により、後述する実施例において使用する枯草菌168株とMGB874株について、蛍光顕微鏡観察によりCTFに由来する赤色蛍光性沈殿を検出することで、測定対象の細胞における呼吸活性の有無及び呼吸活性の持続性を評価した。その結果、MGB874株は、枯草菌168株と比較して優れた呼吸活性の持続性を有しており、枯草菌168株と比較して長寿命であることが判った。また、本実験例では、CTCを用いた染色の後に蛍光顕微鏡によってCTFに由来する赤色蛍光性沈殿を検出したが、実験およびデータの解析には16サンプルにつき少なくとも48時間かかった。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007197754A JP2009028011A (ja) | 2007-07-30 | 2007-07-30 | 細胞呼吸活性の測定方法及びそのキット、タンパク質生産性向上株のスクリーニング方法及びそのキット |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007197754A JP2009028011A (ja) | 2007-07-30 | 2007-07-30 | 細胞呼吸活性の測定方法及びそのキット、タンパク質生産性向上株のスクリーニング方法及びそのキット |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2009028011A JP2009028011A (ja) | 2009-02-12 |
| JP2009028011A5 true JP2009028011A5 (ja) | 2010-07-29 |
Family
ID=40399253
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007197754A Pending JP2009028011A (ja) | 2007-07-30 | 2007-07-30 | 細胞呼吸活性の測定方法及びそのキット、タンパク質生産性向上株のスクリーニング方法及びそのキット |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2009028011A (ja) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63287498A (ja) * | 1987-05-20 | 1988-11-24 | Showa Denko Kk | 血清の検定法 |
| JPH04148680A (ja) * | 1990-10-12 | 1992-05-21 | Asahi Chem Ind Co Ltd | 生理活性物質生産細胞の選択方法 |
| KR100463714B1 (ko) * | 2002-06-27 | 2004-12-29 | 한국생명공학연구원 | 식물생장조절물질의 고효율 스크리닝 방법 |
| CN1291231C (zh) * | 2003-12-08 | 2006-12-20 | 胡军 | 活化淋巴细胞特异性的检测方法 |
| JP4513099B2 (ja) * | 2005-03-31 | 2010-07-28 | 国立大学法人豊橋技術科学大学 | 微生物の汚染物質分解能力測定方法 |
| JP4605559B2 (ja) * | 2005-08-24 | 2011-01-05 | 一啓 木暮 | 細菌活性の観察方法 |
-
2007
- 2007-07-30 JP JP2007197754A patent/JP2009028011A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MY183402A (en) | Quencher containing water-soluble polymer-conjugated nanomaterial and use thereof | |
| BR112013000413A2 (pt) | processo para detectar e identificar diretamente um microrganismo em uma amostra biológica diluída em um caldo de enriquecimento. | |
| MX2009005651A (es) | Marcadores geneticos para el manejo de riesgo de la arritmia cardiaca. | |
| WO2013086429A3 (en) | Methods and compositions for classification of samples | |
| WO2009120964A3 (en) | In vivo flow cytometry based on cellular autofluorescence | |
| EP2757378A3 (en) | Biological substance detection method | |
| EP1934377A4 (en) | METHOD AND COMPOSITIONS FOR IDENTIFYING BIOMARKERS SUITED TO THE DIAGNOSIS AND / OR TREATMENT OF BIOLOGICAL CONDITIONS | |
| WO2011045544A3 (fr) | Procede de caracterisation d'au moins un microorganisme par spectrometrie de masse | |
| DE602007014278D1 (de) | Nachweis und Qualitätskontrolle von Regulator-T-Zellen mittels DNA-Methylierungsanalyse des FoxP3-Gens | |
| MX350533B (es) | Biomarcadores de cancer pancreatico y usos de los mismos. | |
| BRPI0815448A2 (pt) | "avaliação rápida das condições respiratórias superiores" | |
| EA201600085A1 (ru) | Набор для обнаружения соевого события syht0h2 | |
| EA201170933A1 (ru) | Анализ нуклеиновых кислот, полученных из единичных клеток | |
| EA201000427A1 (ru) | Секвенирование нуклеиново-кислотных полимеров с использованием электронной микроскопии | |
| MX355416B (es) | Biomarcadores de cancer de pulmon y usos de los mismos. | |
| BR112012030813A2 (pt) | "métodos e kits para detecção in situ de sequências de nucleotídeo" | |
| NO20091428L (no) | Forbindelser og metoder for 18F-merkede midler | |
| EP4296374A3 (en) | Detection of infectious agents from environmental air dust | |
| MX336093B (es) | Deteccion y enumeracion de microorganismos. | |
| WO2015184498A3 (en) | Method for methylation analysis | |
| WO2012064978A3 (en) | Compositions, methods, and kits for detecting and identifying mycobacteria | |
| WO2012103337A3 (en) | Assays and methods to sequence microbes directly from immune complexes | |
| JP2008545432A5 (ja) | ||
| WO2012135620A3 (en) | Molecular gram stain | |
| JP2009028011A5 (ja) |