JP2009023936A - Antioxidant and cosmetic composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an efficient antioxidant having excellent effects, and being prepared by utilizing antioxidant properties and radical-eliminating properties of an extract of Photinia glabla of natural components by utilizing the extract of the Photinia glabla such as pigments, to enlarge the scope of utilization of the Photinia glabla, and to provide a cosmetic composition using the same. <P>SOLUTION: The antioxidant comprises the extract of the Photinia glabla. The cosmetic composition uses the antioxidant. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an antioxidant and a cosmetic composition containing the antioxidant. More specifically, the present invention aims to provide an effective and effective antioxidant and cosmetic using the anti-oxidant property and radical scavenging property of the natural component, kanamachi extract.

  In Kanamochichi, the bud-blowed new leaves are red and the proportion of green gradually increases, but the red color is maintained almost all year round. Those improved for hedges are particularly vibrant in the color of new leaves, and are also referred to as red crab, and more than 70% of all leaves are red, producing natural red pigments efficiently. It has been reported that the red pigment of this anemone is anthocyanin, and cyanidin-3-glucoside (chrysanthemin) and cyanidin-3-rhamnoglucoside (antilinin) have been reported as anthocyanins in fruits (Non-patent Document 1). ).

  Moreover, considering that the flavonoid patterns of leaves and fruits are similar (Non-patent Document 2) and that new leaves turn red and anthocyanins are produced in angiosperms found in Japan (Non-patent Document 3), red kanamachichi It is thought that anthocyanins accumulate in the new leaves.

  Anthocyanins have been used as a colorant in various foods for a long time because they are safer and have a natural color compared to synthetic colorants. Recently, anthocyanins have been found to exhibit antioxidant properties, radical scavenging properties, and antimutagenic properties, and have been reevaluated as food factors that suppress oxidative stress and DNA mutation in vivo.

  Therefore, taking into consideration the high productivity of red pigments, it is desirable to use the Kanamochi extract, especially the extract containing the pigment, mainly for its physiological activity, including the antioxidant properties. I don't see much. Kanamochi is currently used as hedges, garden trees, etc. However, in order to improve the efficiency of use of kanamechichi, a wide range of application development and technology development including the use of chemical components is desired.

Ishikura, N: Botanical Magazine Tokyo, 88, 41-45 (1975) Ishikura, N: Phytochemistry, 11, 1741-1750 (1972) Ishikura, N: Botanical Magazine Tokyo, 85, 303-306 (1972)

  Accordingly, the object of the present invention is to solve the above-mentioned problems, and by utilizing the Kananachi extract such as a pigment, while expanding the usage form of Kananachi, the excellent antioxidant property of the natural component Kananachi extract And an effective and effective antioxidant and cosmetic composition utilizing radical scavenging properties.

  As a result of intensive studies to achieve the above object, the present inventor has found that the problems of the prior art as described above can be solved by using the kanamachi extract as an antioxidant and a cosmetic composition. The invention has been completed. That is, the present invention provides an antioxidant characterized by containing a kanamachi extract and a cosmetic composition using the same.

  According to the present invention, the use of Kanamochi extract as an antioxidant in a cosmetic composition effectively exhibits antioxidant properties and radical scavenging properties, and has a high anti-aging effect, anti-wrinkle effect, anti-sagging effect, etc. It can be expressed, and at the same time, effective use of kana-memochi is promoted.

  Next, the present invention will be described in more detail with reference to the best mode for carrying out the invention. Kanamochi used in the present invention refers to kanamachichi and its related species, and is an evergreen small tree of the genus Rosaceae, a plant having the scientific name of Photinia glabla, and related plants. Because the new leaves turn red, it is also called red candy.

  In addition, buckwheat, buckwheat, buckwheat and the like may be used as other names, and these are also included in the kana notes used in the present invention. Among the varieties of kana memochi, those that have a red sprouting and later have a lot of new red leaves are known as “benikaname” and “benikana memochi”, and these are also included in the kana memochi used in the present invention.

  Also, as an analog of Kanamochi, there is a plant that is characterized by a larger leaf, and the young leaf has a red name, Pokkinia serrulata, whose name is red-faced like red crab. In addition, there is a garden varieties called Red Robin (Photinia × fraseri) that is a hybrid of Kanamochi and Okanamemochi as a related species of Kanamochi, and it is also called Western beetle or American canamemochi because it was made in the West. Plants with these names are also included in the kana note used in the present invention.

  Varieties that are particularly preferably used in the present invention include red crab and red crab that have a lot of new red leaves. What is used as a bioactive agent in the present invention is an extract from each part such as new leaves, roots, stems, leaves, etc., but it is particularly preferable to use a red-colored new leaf part of a plant. Is an extract from

  The main antioxidants in Kanamochi new leaves include anthocyanins and carotenoids. In Kanamochi, the new leaves turn red, but this red color is due to anthocyanins and is said to protect the new leaves from ultraviolet rays until chlorophyll can be produced. In addition, carotenoid synthesis seems to occur in the red new leaves. Carotenoids are yellow pigments that exist with chlorophyll in chloroplasts, and are said to function in plants such as absorption of light energy, transfer of excitation energy, and protection against photodamage.

  The red leaf of Kanamochi will eventually produce chlorophyll and turn to fresh green. It is known that carotenoid synthesis occurs prior to the production of chlorophyll. Therefore, it is considered that the accumulation of red pigment anthocyanin and yellow pigment carotenoid occurs simultaneously in the new leaves of kanamemochi. Because of the polyphenol structure for anthocyanins and the long conjugated double bond system for carotenoids, these dyes are thought to have strong antioxidant power.

  As a form of kanamemo used as a raw material for antioxidants in the present invention, red-colored new leaves, roots, stems, leaves and the like are cut into appropriate sizes, and from this, water, ethyl alcohol, methyl alcohol, acetic acid An extract is obtained with a suitable extraction medium such as ethyl, acetonitrile, acetone, 1,3-butylene glycol, ethyl ether, petroleum ether, and this extract is used as a cosmetic antioxidant.

  The types of cosmetics in which the antioxidant of the present invention is used include facial cleansing creams, facial cleansing foams, lotions, cosmetic liquids, packs, massage agents, emulsions, lip balms, moisture creams, and other basic cosmetics and foundations. Makeup cosmetics such as white powder, lipstick, eye color, teak color, nail enamel, remover, soap, body shampoo, bathing agent, sunscreen agent, deodorant spray, decoloring cream, hair removal cream, etc. For cosmetics for hair such as cosmetics, shampoos, rinses, hair treatments, hair mousses, hair liquids, pomades, perm waves, hair colors, bleaches, etc., for scalp cosmetics such as hair restorers, hair tonics, scalp treatments Cosmetics, perfume, cologne, Dotoware like fragrance cosmetic cosmetics such as, but not limited thereto.

  Although the compounding quantity to the cosmetics of the antioxidant of this invention is not specifically limited, It is about 0.0001-20 mass parts with respect to 100 mass parts of cosmetics. About 0.001-5 mass parts is especially preferable with respect to 100 mass parts of cosmetics.

  Cosmetics using the antioxidant of the present invention include oily bases such as fats and oils, waxes, hydrocarbons, higher fatty acids, purified water, aqueous bases such as ethyl alcohol, nonionic surfactants, anionic surfactants Surfactants such as agents, polymer bases such as natural polymer substances and synthetic polymer substances, and cosmetic bases such as powder bases such as talc and kaolin are used. Examples of the cosmetic dosage form include, but are not limited to, a solution type, a gel type, an emulsification type, a solid type, a powder type, a paste type, a film type, and an aerosol type.

  The extraction method of the kanamachi extract used as an antioxidant in the present invention may be a normal extraction method, and is not particularly limited as long as the extract has antioxidant ability such as free radical scavenging ability. For example, when extracting using an extraction solvent, water, a hydrophilic organic solvent, or a hydrophobic organic solvent is used as the extraction solvent. For example, water, carbon dioxide, lower alcohols having 1 to 5 carbon atoms, hydrous lower alcohols, esters of lower alcohols having 1 to 5 carbon atoms and fatty acids having 1 to 5 carbon atoms, ketones, and the above esters Solvents such as hydrates of the above ketones, halogenated hydrocarbons, ethers, hydrocarbons, liquefied gas (liquid carbonic acid, liquefied propane, liquefied butane, etc.) are used, but water, ethyl alcohol, methyl alcohol, , 3-butylene glycol, ethyl acetate, acetonitrile, acetone, ethyl ether, petroleum ether, chloroform, benzene, n-butanol, dioxane, tetrahydrofuran, n-hexane and the like are preferably used. Particularly preferably used are water, ethanol and the like. A mixture of these extraction solvents is also preferably used.

  Extraction conditions such as extraction temperature and extraction time are not particularly limited, and normal extraction conditions are used. For example, as a suitable extraction temperature, a temperature in the range of 0 to 90 ° C is used, and a temperature in the range of 5 to 70 ° C is particularly preferably used. As the suitable extraction time, an extraction time in the range of 1 hour to 1 week is used, and in particular, an extraction time in the range of 1 day to 3 days is preferably used. As for a preferable solvent capacity, a solvent amount in the range of 1 to 100 times by mass with respect to the extract is suitably used, and particularly a solvent amount of 2 to 50 times is suitably used.

  The kanamachi extract can be obtained by directly or concentrating the extract obtained by extraction with the above solvent. Extraction means is not particularly limited, for example, a method of immersing Kanakichi raw materials such as new leaves, leaves, stems, roots, and the like in the solvent at room temperature, and after leaving the Kanakichi raw materials in the solvent, A method of extracting with heating and stirring at a temperature below the boiling point of the solvent, a supercritical extraction method, a subcritical extraction method, and the like are also used.

  The form of use of the antioxidant of the present invention is not particularly limited, but the above kanamachi extract may be used as a liquid such as a solution, a dispersion, or an emulsion, together with various additives as necessary. Used as a cosmetic as a solid such as a film or sheet. Additives include water, oils, surfactants, lubricants, alcohols, water-soluble polymer agents, gelling agents, moisturizers, buffers, preservatives, anti-inflammatory agents, thickeners, fragrances, vitamins And other antioxidants, but are not limited to these. These additives may be used alone or in combination of two or more.

  The kanamachi extract of the present invention can be used as it is or as an antioxidant, and can also be used by adding it to a conventionally known antioxidant base material. Although the usage-amount with respect to an antioxidant base material is not specifically limited, When using together with an antioxidant base material, it is about 10-100 mass parts with respect to 100 mass parts of antioxidant base materials as a standard. In particular, about 50-100 mass parts is preferable with respect to 100 mass parts of antioxidant base materials.

  The cosmetic composition in the present invention refers to a preparation prepared by blending an essential ingredient, kanamachi extract, and various forms of bases known as normal external preparations for skin, for local or systemic use. It can be made into cosmetics such as cleaning agents or cosmetics for skin, scalp and hair, bath preparations, deodorant / deodorant / deodorants, cosmetic sheets, hygiene products and the like. Examples of the cosmetic product include, but are not limited to, ampoules, capsules, powders, granules, solids, solutions, gels, dispersions, emulsions, sheets, mists, sprays, and the like.

  The cosmetic products include, for example, emulsions, creams, lotions, ointments, cosmetics, gels, lotions, cosmetic oils, packs, mists, facial cosmetic sheets, and other basic cosmetics, bath preparations, cleaning agents, and skins. Body cosmetics such as cleaning agents, deodorants, antiperspirants, cleansing agents, sunscreens, makeup cosmetics such as foundation, lipstick, blusher, eye shadow, eyeliner, mascara, shampoo, rinse, hair treatment, hair styling Hair, scalp cosmetics, hair tonics, hair dyes, hair / scalp cosmetics such as hair growth / hair nourishing agents, perfumes, and fragrance cosmetics such as eau de cologne.

  In the cosmetic composition of the present invention, water, an oil agent, fats and oils, waxes, fatty acids, mineral oils, surfactants, metals as components that are usually used as long as they do not impair the effects of the present invention. Soap, gelling agent, powder, alcohols, polyhydric alcohols, esters, water-soluble polymers, film forming agents, resins, gums, sugars, inclusion compounds, vitamins, amino acids, pH regulators, Antibacterial agents, antiseptics, other antioxidants, blood circulation promoters, moisturizers, cooling agents, antioxidants, fragrances, and the like can be added.

  Although the usage-amount of the kana memochi extract used for the cosmetic composition of this invention is not limited, with respect to 100 mass parts of cosmetics, a kana memo extract is used by about 0.00001-20 mass parts as a dry part, In particular, it is preferably used in the range of 0.0005 to 5 parts by mass. In the case of an extract, it is used in an amount equivalent to this dry matter.

  Antioxidant expression by an extract derived from kanamemochi is considered to be performed by the following mechanism. In general, antioxidation is defined as a property that resists the reaction of depriving electrons. Specifically, the hydrogen abstraction reaction by active oxygen and free radicals that cause inconvenience for the living body in various forms, and the activity. It has the property of stopping the radical chain reaction caused by oxygen and free radicals. In this case, active oxygen is a generic term for triplet oxygen (oxygen molecules in the atmosphere) that has changed to a more reactive substance, but in general, superoxide anion radical, hydroxy radical, singlet oxygen, Four types with particularly strong reactivity and toxicity of hydrogen peroxide.

  Here, the superoxide anion radical and the hydroxy radical are free radicals. A free radical is defined as a molecule or atom having one or more unpaired electrons, but a hydrogen radical, which is a free radical such as ultraviolet rays, is also generated from hydrogen peroxide. Superoxide anion radicals also react quickly with hydrogen peroxide to generate hydroxy radicals. Hydroxyl radicals react with lipids that make up cell membranes relatively easily, change lipids into highly toxic lipid peroxides, cause reactions one after another, and damage biological components. In addition, these active oxygens and free radicals denature the intercellular matrix in the dermis of the skin and damage the fibroblasts constituting the dermis.

  The intercellular matrix is composed of collagen, elastin, fibronectin, mucopolysaccharide, proteoglycan, etc., but when the collagen structure with a regular fiber structure is destroyed by active oxygen and free radicals, the tension of the skin disappears and wrinkles Contributes to the formation. Furthermore, it is considered that elastin related to skin elasticity and stretching is also easily decomposed by active oxygen and free radicals, resulting in skin aging such as wrinkles, sagging and reduced flexibility. In addition, if the DNA of fibroblasts is damaged by active oxygen and free radicals, it causes skin cancer.

  The flavonoids in the extract of kanamemochi such as anthocyanin, which is a pigment mainly exhibiting red and purple, have the above-mentioned properties due to the hydrogen-donating property of the phenolic hydroxyl group in the structure and the radical coupling property by radicals generated by the hydrogen donation. It is thought that it eliminates free radicals such as hydroxy radicals and superoxide anion radicals, exhibits antioxidant properties, and protects against damage to hydroxy radicals, superoxide anion radicals, and even biological components derived from hydrogen peroxide.

  On the other hand, for singlet oxygen, the singlet oxygen is bonded to the carbon at the 2-position of anthocyanidin derived from Kanametti to generate hydroperoxide, and further, the B ring portion is separated to generate a benzoic acid derivative. It is considered that the reactivity is inactivated, and it effectively functions in complementing oxidation protection systems such as SOD and catalase in the skin. In addition to the above-mentioned radicals, it is considered to effectively act to eliminate free radicals such as lipid peroxyl radicals, alkoxy radicals, hydroperoxy radicals, nitric oxide and nitrogen dioxide.

  In addition, carotenoids, which are mainly yellowish pigments in extracts from kanamemochi, are also effective in eliminating singlet oxygen and free radicals. The elimination of singlet oxygen is considered to be realized by energy transfer from the singlet oxygen to the carotenoids derived from kanamemochi and, as a result, the carotenoid that is in the triplet excited state returns to the ground state by vibrational relaxation. Regarding free radical elimination, it is considered that carotenoids function by directly exchanging electrons with hydroxy radicals and other free radicals described above, thereby preventing injury.

  An extract from kanamemochi, combined with the effects of the above-mentioned anthocyanins and carotenoids, works to eliminate most of the active oxygen and free radicals as a whole, resulting in aging (wrinkles, sagging, reduced flexibility, etc.) Various symptoms caused by active oxygen and free radicals such as inflammation, disease, and the like can be alleviated.

Hereinafter, the present invention will be described in more detail with reference to examples. In the text, “%” is based on mass.
<Example 1> Kanamechi (Benikaname) New Leaf-Derived Extract A red-colored kanamemochi (Benikaname) new leaf was cut out and 10 grams thereof was immersed in 100 ml of ethanol overnight. Thereafter, the immersion liquid was filtered through a qualitative filter paper (No. 2) to obtain an extract. The extraction residue was further subjected to extraction filtration in the same manner with 100 ml of ethanol to obtain 300 ml of an extract. Ethanol was distilled off from the extract with a rotary evaporator, and after drying, 1.2 g of red-brown kanamachichi new leaf-derived extract was obtained.

<Example 2> Thin-layer chromatography (TLC) analysis of an extract derived from new leaves of Kanakachi (Benikame) Silica gel 60F254TLC aluminum plate (5 × 20 cm, manufactured by Merck) Example 1) An ethanol solution was spotted and developed by an ascending method at a room temperature of 23 ° C. The developing solvent was BAW (n-butanol: acetic acid: water = 4: 1: 5 upper layer) and developed for about 3 hours. After air drying for about 3 minutes with a hair dryer, the Rf value and color tone of the spot that appeared were examined. The results are shown in Table 1. From this result, it became clear that there are at least five types of pigments having red, purple, and yellow color tones in the extract from new leaves of Kanamochi.

<Example 3> Radical scavenging ability of an extract from new leaves of Japanese red spruce (Benikame) The scavenging ability of DPPH (1,1-diphenyl-2-pycrylhydrazyl) radical was measured, and the antioxidant property of the sample was analyzed spectroscopically. .
1.0 g of an extract from anemone leaf of Example 1 was dissolved in 5 ml of water to obtain a stock solution of an extract solution from anemone leaf. Using a 96-well microplate, 20 microliters of an extract solution derived from new leaves of Kanamochichi appropriately diluted with 50% aqueous ethanol solution, 125 microliters of 0.2 mM DPPH (ethanol solution) and 125 microliters of 0.2 M MES (2 -A morpholinoethanesulfonic acid aqueous solution (pH 6.0) was added, and the mixture was allowed to stand at room temperature. After 20 minutes, the absorbance at 540 nanometers was measured. As a control, a 50% ethanol aqueous solution was used instead of the sample solution, and since the sample was colored, a blank obtained by adding 250 microliters of 50% ethanol aqueous solution to the sample solution was used as a blank. The radical scavenging activity was calculated by the following formula using each absorbance.
Radical scavenging activity (%) = (1− (sample−blank) / control) × 100
The results are shown in Table 2. From this result, it was clarified that the extract derived from new leaves of Kanamochi has strong radical scavenging ability.

<Example 4> SOD activity of an extract derived from new leaves of kana memochi (Benikaname) An extract solution derived from new leaves of kana memochi prepared by appropriately diluting the stock solution of extract of kana memochi new leaves from example 3 with 50% aqueous ethanol. Used, the SOD activity of the extract from the new leaves of Kanamochi was measured. For the measurement, an SOD activity measurement kit SOD Assay Kit (manufactured by dojindo) based on the detection principle that xanthine-xanthine oxidase was used as a superoxide generation system and the generated superoxide produced water-soluble formazan was used. For control, pure water was used instead of the sample solution. Moreover, since the sample was colored, it measured simultaneously as a blank using the buffer for dilution (attached to a kit) instead of xanthine oxidase. SOD activity (inhibition rate) was calculated by the following equation using each absorbance.
SOD activity (inhibition rate) (%) = (1− (sample−blank) / control) × 100
The results are shown in Table 3. From this result, it was clarified that the extract derived from new leaves of Kanamochi has strong SOD activity.

The formulation example of the cosmetic composition according to the present invention is shown below.
Formulation Example 1 (Lotion)
-Glycerin 5.0 parts by mass-Propylene glycol 4.0 parts by mass-1% Kanamochi new leaf-derived extract (Example 1) 1,3 butylene glycol solution
2.0 parts by mass, polyoxyethylene lauryl ether (20E.O.) 1.0 part by mass, ethanol 10.0 parts by mass, antioxidant, appropriate amount, preservative, appropriate amount, fragrance, appropriate amount, purified water balance
Total 100 parts by mass

Formulation Example 2 (Cream)
-5.0 parts by weight of glycerin-5.0 parts by weight of liquid paraffin-5.0 parts by weight of isopropyl myristate-1% kanamachichi leaf extract (Example 1) 1,3 butylene glycol solution
2.0 parts by weight, polyoxyethylene hydrogenated castor oil 5.0 parts by weight, ethanol 5.0 parts by weight, antioxidant appropriate amount, preservative appropriate amount, perfume appropriate amount, purified water balance
Total 100 parts by mass

  According to the present invention, the use of Kanamochi extract as an antioxidant in a cosmetic composition effectively exhibits antioxidant properties and radical scavenging properties, and exhibits high anti-aging effect, anti-wrinkle effect, anti-sagging effect, etc. It can be expressed, and at the same time, effective use of kana-memochi is promoted.

Claims (3)

  A radical scavenging antioxidant containing a kanamachi extract.   A reactive oxygen scavenging antioxidant characterized by containing an extract of kanamechi.   A cosmetic composition comprising the antioxidant according to claim 1 or 2.