JP2008546422A - Ｄｎａポリメラーゼブレンドおよび突然変異型ｄｎａポリメラーゼ - Google Patents

Ｄｎａポリメラーゼブレンドおよび突然変異型ｄｎａポリメラーゼ Download PDF

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JP2008546422A
JP2008546422A JP2008519378A JP2008519378A JP2008546422A JP 2008546422 A JP2008546422 A JP 2008546422A JP 2008519378 A JP2008519378 A JP 2008519378A JP 2008519378 A JP2008519378 A JP 2008519378A JP 2008546422 A JP2008546422 A JP 2008546422A
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dna
exo
polymerase
primer
dna polymerase
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JP2008546422A5 (enExample
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ウェイドン チェン
チュン イー． リー
ロバート ジェイソン ポッター
デイビッド マンデルマン
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インヴィトロジェン コーポレーション
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
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  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
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  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
JP2008519378A 2005-06-28 2006-06-20 Ｄｎａポリメラーゼブレンドおよび突然変異型ｄｎａポリメラーゼ Pending JP2008546422A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/170,762 US20060292578A1 (en) 2005-06-28 2005-06-28 DNA polymerase blends and mutant DNA polymerases
PCT/US2006/023899 WO2007002031A2 (en) 2005-06-28 2006-06-20 Dna polymerase blends and mutant dna polymerases

Publications (2)

Publication Number Publication Date
JP2008546422A true JP2008546422A (ja) 2008-12-25
JP2008546422A5 JP2008546422A5 (enExample) 2012-07-12

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JP2008519378A Pending JP2008546422A (ja) 2005-06-28 2006-06-20 Ｄｎａポリメラーゼブレンドおよび突然変異型ｄｎａポリメラーゼ

Country Status (6)

Country Link
US (3) US20060292578A1 (enExample)
EP (2) EP1904518B1 (enExample)
JP (1) JP2008546422A (enExample)
AT (1) ATE484515T1 (enExample)
DE (1) DE602006017551D1 (enExample)
WO (1) WO2007002031A2 (enExample)

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US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
US9617588B2 (en) 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
US8932813B2 (en) 2010-12-13 2015-01-13 Life Technologies Corporation Polymerization of nucleic acids using activation by polyphosphorolysis (APP) reactions
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
FI3461807T3 (fi) 2011-06-08 2023-09-07 Life Technologies Corp Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten
DK2914741T3 (da) 2012-11-02 2017-11-20 Life Technologies Corp Hidtil ukendte sammensætninger og fremgangsmåder til forbedring af PCR-specificitet
TWI487789B (zh) * 2012-12-24 2015-06-11 Ind Tech Res Inst 單離的脫氧核糖核酸聚合酶、套組與其應用
KR101417989B1 (ko) * 2013-02-08 2014-07-09 주식회사 엔지노믹스 이중가닥 dna 말단의 단일가닥 길이 조절 방법
EP2971095B1 (en) 2013-03-12 2019-11-20 Life Technologies Corporation Universal reporter-based genotyping methods, reaction mixture and kit
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
EP3967768A1 (en) 2015-03-13 2022-03-16 Life Technologies Corporation Compositions for small rna capture, detection and quantification
WO2025235589A1 (en) * 2024-05-08 2025-11-13 Codexis, Inc. Dna polymerase variants

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Publication number Priority date Publication date Assignee Title
JP2001513983A (ja) * 1997-08-29 2001-09-11 ライフ テクノロジーズ，インコーポレイテッド 高忠実度ポリメラーゼおよびその使用
WO2004058942A2 (en) * 2002-12-20 2004-07-15 Stratagene Dna polymerase blends and uses thereof
JP2005508630A (ja) * 2001-09-14 2005-04-07 インヴィトロジェン コーポレーション Ｄｎａポリメラーゼとその変異体
JP2005511043A (ja) * 2001-11-30 2005-04-28 アプレラ コーポレイション Ｔｈｅｒｍｕｓｔｈｅｒｍｏｐｈｉｌｕｓ核酸ポリメラーゼ
JP2005516611A (ja) * 2001-09-14 2005-06-09 アプレラ コーポレイション Ｔｈｅｒｍｕｓｓｃｏｔｏｄｕｃｔｕｓ核酸ポリメラーゼ

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JP2001513983A (ja) * 1997-08-29 2001-09-11 ライフ テクノロジーズ，インコーポレイテッド 高忠実度ポリメラーゼおよびその使用
JP2005508630A (ja) * 2001-09-14 2005-04-07 インヴィトロジェン コーポレーション Ｄｎａポリメラーゼとその変異体
JP2005516611A (ja) * 2001-09-14 2005-06-09 アプレラ コーポレイション Ｔｈｅｒｍｕｓｓｃｏｔｏｄｕｃｔｕｓ核酸ポリメラーゼ
JP2005511043A (ja) * 2001-11-30 2005-04-28 アプレラ コーポレイション Ｔｈｅｒｍｕｓｔｈｅｒｍｏｐｈｉｌｕｓ核酸ポリメラーゼ
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Also Published As

Publication number Publication date
ATE484515T1 (de) 2010-10-15
DE602006017551D1 (de) 2010-11-25
EP2314596A2 (en) 2011-04-27
WO2007002031A3 (en) 2007-07-26
EP1904518A4 (en) 2009-03-04
EP2314596B1 (en) 2013-06-05
EP2314596A3 (en) 2011-07-06
EP1904518A2 (en) 2008-04-02
US20160272955A1 (en) 2016-09-22
US20060292578A1 (en) 2006-12-28
EP1904518B1 (en) 2010-10-13
WO2007002031A2 (en) 2007-01-04
US20080254525A1 (en) 2008-10-16
US9376698B2 (en) 2016-06-28

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