JP2008538813A - マイクロ流体デバイス用の小型光学検出システム - Google Patents
マイクロ流体デバイス用の小型光学検出システム Download PDFInfo
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Abstract
Description
本出願は米国仮特許出願第60/670,736号、2005年4月12日出願、標題「マイクロ流体デバイスのための光学検出(Optical Detection for Microfluidic Devices)」の優先権を主張すると共に該米国仮特許出願をあらゆる目的のためここで援用する。
一般に本発明は検出光学の分野に関する。特に、本発明は蛍光信号を検出するための小型光学システムと、小型光学検出システムに適合するドライフォーカス式マイクロ流体デバイスとに関するものである。
本発明の態様の一つは、マイクロ流体デバイス用の光学検出システムである。本システムは発光ダイオード(LED)と;LEDにより発せられた光を平行にするための手段と;非球面の溶融石英製対物レンズと;平行光を対物レンズを通してマイクロ流体デバイス上に向かわせるための手段と;マイクロ流体デバイスから発せられる蛍光信号を検出するための手段とを備える。システムは第2の外部光源を含むこともでき、これはLED又はレーザーでよい。対物レンズからマイクロ流体デバイスまでの作動距離の大きさは、LED又はレーザーからの光が対角経路に沿って取り込まれてマイクロ流体デバイスを照射できるような大きさとし得る。
図面の簡単な説明
110 LED光源
120 スリット
130 スリットレンズ
140 励起帯域通過フィルター
150 ビームスプリッター
160 対物レンズ
165 マイクロ流体デバイス
170 除去フィルター
180 回折格子
190 CCDレンズ
195 CCDアレー
Claims (26)
- 第1の基板と;
少なくとも1つのチャンネルがマイクロ流体チャンネルである前記第1の基板内に形成された複数のチャンネルと;
前記複数のチャンネルから間隔をあけて前記第1の基板内に形成された複数の光学位置合せマークであって、該位置合せマークの各々が湾曲壁を有する複数の光学位置合せマークと;
前記チャンネルが覆われると共に前記位置合せマークが閉鎖されるように前記第1の基板に接着される第2の基板と;
を備え、少なくとも1つのチャンネルが少なくとも2つの位置合わせマークの間に配置されることを特徴とするドライフォーカス式マイクロ流体デバイス。 - 前記光学位置合せマークが2つの列を形成し、前記複数のチャンネルが少なくとも2つの平行なチャンネルを含み、前記平行なチャンネルが前記2つの列の間に配置される、請求項1に記載のデバイス。
- 前記光学位置合せマークがエッチング、スタンピング、エンボス加工、モールディング、レーザーアブレーション、及びそれらの組み合わせからなる群から選択された方法によって形成される、請求項1に記載のデバイス。
- 前記光学位置合せマークが等方性エッチ液と10x10μmの正方形のマスクとを用いて前記基板中にエッチングされる、請求項1に記載のデバイス。
- 前記デバイスが12個以上の平行なチャンネルと22個以上の光学位置合せマークとを含み、前記位置合せマークが各列11個の光学位置合せマークを2列形成し、該列が前記チャンネルの外側にて前記チャンネルに平行に配置される、請求項1に記載のデバイス。
- 発光ダイオードと;
発光ダイオードにより発せられる光を平行にするための平行化手段と;
非球面の溶融石英製対物レンズと;
平行光を対物レンズを通してマイクロ流体デバイス上に向かわせるための方向付け手段と;
マイクロ流体デバイスから発せられる蛍光信号を検出するための検出手段と
を備えるマイクロ流体デバイス用の光学検出システム。 - 前記対物レンズの開口数が0.5である請求項6に記載のシステム。
- 前記平行化手段がスリットとスリットレンズとを有する請求項6に記載のシステム。
- 前記方向付け手段が二色性ビームスプリッターを有する請求項6に記載のシステム。
- 前記検出手段がCCDを有する請求項6に記載のシステム。
- CCDレンズを更に備える請求項10に記載のシステム。
- 前記CCDレンズがトリプレットレンズ素子を有する請求項12に記載のシステム。
- 集光レンズ、励起帯域通過フィルター、除去フィルター、回折格子、ビームスプリッター、折り畳みミラー、及び放射帯域通過フィルターのうち1つ以上を更に備える請求項6に記載のシステム。
- 前記LEDから発せられる光が軸方向に前記マイクロ流体デバイスまで送られる請求項6に記載のシステム。
- 外部光源を更に備える請求項6に記載のシステム。
- 前記対物レンズから前記マイクロ流体デバイスまでの作動距離の大きさが、前記外部光源から斜角にて送り込まれた光によって前記マイクロ流体デバイスが照射されるような大きさである、請求項15に記載のシステム。
- 前記対物レンズから前記マイクロ流体デバイスまでの作動距離が16mmである請求項16に記載のシステム。
- 前記外部光源が発光ダイオード及びレーザーのうちの一方である請求項15に記載のシステム。
- 前記マイクロ流体デバイスが請求項1に記載のドライフォーカス式マイクロ流体デバイスであり、前記位置合せマークが前記外部光源により照射される、請求項15に記載のシステム。
- 前記外部光源が白色LEDである請求項19に記載のシステム。
- コンピュータ使用可能な媒体を更に備える請求項19に記載のシステム。
- 前記コンピュータ使用可能な媒体がオートフォーカス・アルゴリズムを有するプログラムを含む、請求項19に記載のシステム。
- 前記コンピュータ使用可能な媒体が検出波長を設定するためのコンピュータプログラムコードを含む、請求項20に記載のシステム。
- 前記コンピュータ使用可能な媒体がスリットからレンズまでの距離を設定するためのコンピュータプログラムコードを含む、請求項20に記載のシステム。
- 光学検出システムの位置合わせ及び焦点合わせを行なうための方法であって、
複数の光学位置合せマークと基板中にエッチングされた複数のチャンネルとを有するマイクロ流体デバイスを提供し、前記チャンネルの少なくとも1つはマイクロ流体チャンネルであり;
前記光学位置合せマークの上部よりも底部が前記光学検出システムの光学素子系に近くなるように、前記光学検出システムの光学素子系に対して前記デバイスを配置し;
外部の白色LEDからの光を斜角にて取り込んで前記光学位置合せマークを照射し;
前記光学検出システムのデータ収集をライン・モードに設定し;
前記光学位置合せマークから反射された光を用いて前記光学検出システムの位置合わせを行い;
前記光学位置合せマークから反射された光を用いて前記光学検出システムの焦点合わせを行い;
前記光学位置合せマークについてのラインデータを、前記チャンネルに対する前記光学位置合せマークの間隔についての光学検出システム内常駐のデータと組み合わせて前記チャンネルからの蛍光信号を感知するように前記光学検出システムを配置することからなる方法。 - 前記光学位置合せマークが等方性エッチ液と10x10μmの正方形のマスクとを用いて前記基板中にエッチングされる、請求項25に記載の方法。
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US67073605P | 2005-04-12 | 2005-04-12 | |
US60/670,736 | 2005-04-12 | ||
PCT/US2006/014313 WO2006115863A2 (en) | 2005-04-12 | 2006-04-12 | Compact optical detection system for microfluidic devices |
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JP2011035075A Active JP5078106B2 (ja) | 2005-04-12 | 2011-02-21 | マイクロ流体デバイス用の小型光学検出システム |
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US (1) | US7518726B2 (ja) |
EP (2) | EP2453223B1 (ja) |
JP (2) | JP5069220B2 (ja) |
CN (1) | CN101663576B (ja) |
AU (1) | AU2006240184A1 (ja) |
CA (1) | CA2603759A1 (ja) |
WO (1) | WO2006115863A2 (ja) |
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KR100953474B1 (ko) | 2008-06-28 | 2010-04-16 | 건국대학교 산학협력단 | 미세유동칩과 마이크로 piv 시스템을 이용한 전자인쇄용잉크의 레올로지 모델 선정방법 |
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US20060227325A1 (en) | 2006-10-12 |
US7518726B2 (en) | 2009-04-14 |
JP5078106B2 (ja) | 2012-11-21 |
EP1880197A2 (en) | 2008-01-23 |
JP2011141286A (ja) | 2011-07-21 |
WO2006115863A3 (en) | 2009-09-24 |
EP1880197B1 (en) | 2019-06-12 |
AU2006240184A1 (en) | 2006-11-02 |
WO2006115863A2 (en) | 2006-11-02 |
EP2453223A1 (en) | 2012-05-16 |
CN101663576A (zh) | 2010-03-03 |
EP2453223B1 (en) | 2019-07-03 |
CA2603759A1 (en) | 2006-11-02 |
CN101663576B (zh) | 2012-05-23 |
JP5069220B2 (ja) | 2012-11-07 |
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