JP2008536518A5 - - Google Patents
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- Publication number
- JP2008536518A5 JP2008536518A5 JP2008507804A JP2008507804A JP2008536518A5 JP 2008536518 A5 JP2008536518 A5 JP 2008536518A5 JP 2008507804 A JP2008507804 A JP 2008507804A JP 2008507804 A JP2008507804 A JP 2008507804A JP 2008536518 A5 JP2008536518 A5 JP 2008536518A5
- Authority
- JP
- Japan
- Prior art keywords
- sample
- absence
- amplification
- detecting
- target organism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000523 sample Substances 0.000 claims description 39
- 230000003321 amplification Effects 0.000 claims description 28
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 27
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 8
- 150000007523 nucleic acids Chemical group 0.000 claims description 8
- 230000001419 dependent effect Effects 0.000 claims description 5
- 239000012468 concentrated sample Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 3
- 230000006037 cell lysis Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000003935 denaturing gradient gel electrophoresis Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000000123 temperature gradient gel electrophoresis Methods 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 1
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229910052726 zirconium Inorganic materials 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67292205P | 2005-04-19 | 2005-04-19 | |
| US70788605P | 2005-08-12 | 2005-08-12 | |
| PCT/US2006/014552 WO2006113706A2 (en) | 2005-04-19 | 2006-04-18 | Increased sensitivity of nucleic acid-based detection of organisms by fractionation of target genomes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008536518A JP2008536518A (ja) | 2008-09-11 |
| JP2008536518A5 true JP2008536518A5 (cg-RX-API-DMAC10.html) | 2009-06-04 |
Family
ID=36972850
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008507804A Pending JP2008536518A (ja) | 2005-04-19 | 2006-04-18 | 標的ゲノムの分画による生物の核酸ベースの検出の感度の増大 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US20060281103A1 (cg-RX-API-DMAC10.html) |
| EP (1) | EP1874963A2 (cg-RX-API-DMAC10.html) |
| JP (1) | JP2008536518A (cg-RX-API-DMAC10.html) |
| BR (1) | BRPI0612464A2 (cg-RX-API-DMAC10.html) |
| CA (1) | CA2604401A1 (cg-RX-API-DMAC10.html) |
| WO (1) | WO2006113706A2 (cg-RX-API-DMAC10.html) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006044728A2 (en) * | 2004-10-18 | 2006-04-27 | U.S.Genomics, Inc. | Methods for isolation of nucleic acids from prokaryotic spores |
| US9670479B2 (en) | 2013-03-15 | 2017-06-06 | F Cubed, LLC | Sample preparation device and methods of use |
| EP3573756A1 (en) | 2017-01-30 | 2019-12-04 | Safeguard Biosystems Holdings Ltd. | Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1317860C (en) * | 1987-04-01 | 1993-05-18 | Daniel Louis Kacian | Techniques for preparing specimens for bacterial assays |
| AU2002354838B2 (en) * | 2001-07-19 | 2007-11-29 | Infectio Diagnostic (I.D.I.) Inc. | Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection |
-
2006
- 2006-04-18 CA CA002604401A patent/CA2604401A1/en not_active Abandoned
- 2006-04-18 US US11/379,084 patent/US20060281103A1/en not_active Abandoned
- 2006-04-18 WO PCT/US2006/014552 patent/WO2006113706A2/en not_active Ceased
- 2006-04-18 EP EP06750559A patent/EP1874963A2/en not_active Withdrawn
- 2006-04-18 BR BRPI0612464-0A patent/BRPI0612464A2/pt not_active IP Right Cessation
- 2006-04-18 JP JP2008507804A patent/JP2008536518A/ja active Pending
- 2006-04-18 US US11/918,610 patent/US20090042189A1/en not_active Abandoned
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