JP2008516637A - 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み - Google Patents

真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み Download PDF

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Publication number
JP2008516637A
JP2008516637A JP2007538145A JP2007538145A JP2008516637A JP 2008516637 A JP2008516637 A JP 2008516637A JP 2007538145 A JP2007538145 A JP 2007538145A JP 2007538145 A JP2007538145 A JP 2007538145A JP 2008516637 A JP2008516637 A JP 2008516637A
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Prior art keywords
trna
amino acid
protein
unnatural amino
amino acids
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Abandoned
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JP2007538145A
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Japanese (ja)
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JP2008516637A5 (https=
Inventor
ピーター・ジー．・シュルツ
サラ・アール．・ハンソン
ラン・スー
ツィーウェン・ツァング
カイ−ヒューイ・ウォン
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Scripps Research Institute
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Scripps Research Institute
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Publication of JP2008516637A publication Critical patent/JP2008516637A/ja
Publication of JP2008516637A5 publication Critical patent/JP2008516637A5/ja
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
JP2007538145A 2004-10-20 2005-10-20 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み Abandoned JP2008516637A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62089804P 2004-10-20 2004-10-20
PCT/US2005/038250 WO2006045116A2 (en) 2004-10-20 2005-10-20 In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria

Publications (2)

Publication Number Publication Date
JP2008516637A true JP2008516637A (ja) 2008-05-22
JP2008516637A5 JP2008516637A5 (https=) 2008-12-11

Family

ID=36203749

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007538145A Abandoned JP2008516637A (ja) 2004-10-20 2005-10-20 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み

Country Status (4)

Country Link
US (3) US20080064059A1 (https=)
EP (1) EP1809758A4 (https=)
JP (1) JP2008516637A (https=)
WO (1) WO2006045116A2 (https=)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7083970B2 (en) * 2001-04-19 2006-08-01 The Scripps Research Institute Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
WO2004094593A2 (en) 2003-04-17 2004-11-04 The Scripps Research Institute Expanding the eukaryotic genetic code
WO2005019415A2 (en) 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
US20050287639A1 (en) 2004-05-17 2005-12-29 California Institute Of Technology Methods of incorporating amino acid analogs into proteins
EP1807515A4 (en) 2004-10-27 2008-06-18 Scripps Research Inst Orthogonal translation components for the in vivo incorporation of unnatural amino acids
US8367588B2 (en) * 2005-10-12 2013-02-05 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
EP1808442B1 (en) * 2006-01-13 2011-05-11 Institut Pasteur Enzymatic large-scale synthesis of mucin glyconjugates, and immunogenic applications thereof
AU2007224019A1 (en) 2006-03-03 2007-09-13 California Institute Of Technology Site-specific incorporation of amino acids into molecules
US7960141B2 (en) * 2006-03-09 2011-06-14 The Scripps Research Institute Systems for the expression of orthogonal translation components in eubacterial host cells
KR20080113226A (ko) 2006-03-16 2008-12-29 더 스크립스 리서치 인스티튜트 비천연 아미노산 페닐셀레노시스테인을 함유하는 단백질의 유전자적으로 프로그램된 발현
US7807411B2 (en) * 2006-05-23 2010-10-05 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
EP2052083B1 (en) * 2006-09-21 2014-01-08 The Scripps Research Institute Genetically programmed expression of selectively sulfated proteins in eubacteria
JP2010506591A (ja) 2006-10-18 2010-03-04 ザ スクリップス リサーチ インスティテュート 哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み
GB0716719D0 (en) * 2007-08-29 2007-10-10 Chromatide Ltd Process for carrying out a chemical reaction
MX2010004464A (es) 2007-10-25 2010-06-07 Scripps Research Inst Incorporacion genetica de 3-aminotirosina a reductasas.
US9150849B2 (en) 2007-11-02 2015-10-06 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
AU2008335778B2 (en) 2007-12-11 2014-04-10 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris
CN101970005A (zh) * 2008-02-08 2011-02-09 斯克利普斯研究院 用遗传编码的非天然氨基酸打破免疫耐受
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
CN102307905B (zh) 2008-12-10 2015-11-25 斯克利普斯研究院 利用化学反应性非天然氨基酸产生载体-肽偶联物
WO2011078987A1 (en) 2009-12-21 2011-06-30 Trustees Of Dartmouth College Recombinant prokaryotes and use thereof for production of o-glycosylated proteins
US12359192B2 (en) * 2013-12-23 2025-07-15 University Of Rochester Methods and compositions for display of macrocyclic peptides
EP3087088B1 (en) * 2013-12-23 2022-05-25 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
US11085045B2 (en) * 2015-11-17 2021-08-10 Anima Biotech Inc. Functional tRNA-aptamer molecules
US20200317708A1 (en) * 2017-10-09 2020-10-08 Indiana University Research And Technology Corporation Sugar-linked amino acids for solid-phase peptide synthesis
US20220299522A1 (en) * 2019-08-26 2022-09-22 The Johns Hopkins University Compositions and methods for identifying o-linked glycosylation sites in proteins
WO2021165968A1 (en) * 2020-02-20 2021-08-26 B. G. Negev Technologies And Applications Ltd., At Ben-Gurion University Mutant aminoacyl-trna synthetases
IL303886A (en) * 2020-12-23 2023-08-01 Flagship Pioneering Inc Compositions of modified trems and uses thereof

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016449A1 (en) * 1990-04-16 1991-10-31 The Trustees Of The University Of Pennsylvania Saccharide compositions, methods and apparatus for their synthesis
US5369017A (en) * 1994-02-04 1994-11-29 The Scripps Research Institute Process for solid phase glycopeptide synthesis
US7083970B2 (en) 2001-04-19 2006-08-01 The Scripps Research Institute Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
EP2042196B1 (en) 2001-10-10 2016-07-13 ratiopharm GmbH Remodelling and glycoconjugation of Granulocyte Colony Stimulating Factor (G-CSF)
ES2324608T3 (es) 2002-10-16 2009-08-11 The Scripps Research Institute Incorporacion especifica de sitio de cetoaminoacidos en proteinas.
MXPA05003978A (es) 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
JP5319863B2 (ja) 2002-12-26 2013-10-16 独立行政法人理化学研究所 体細胞相同組換えの誘発方法
WO2004094593A2 (en) 2003-04-17 2004-11-04 The Scripps Research Institute Expanding the eukaryotic genetic code
WO2005019415A2 (en) 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
US20060160175A1 (en) 2003-07-07 2006-07-20 The Scripps Research Institute Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof

Also Published As

Publication number Publication date
WO2006045116A2 (en) 2006-04-27
US20080064059A1 (en) 2008-03-13
EP1809758A4 (en) 2009-04-01
EP1809758A2 (en) 2007-07-25
US7378263B2 (en) 2008-05-27
US20080241903A1 (en) 2008-10-02
WO2006045116A3 (en) 2006-12-28
US20060110796A1 (en) 2006-05-25

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