JP2008214211A - Cathartic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To contribute to the stable supply of the active ingredient of a cathartic derived from a plant of the genus Rheum. <P>SOLUTION: The cathartic comprises an anthraquinone analog as an active ingredient extracted from the terrestrial part of a plant of the genus Rheum. Since the whole from the subterranean part to the terrestrial part of one plant of the genus Rheum is effectively used without waste, more active ingredients are extracted from one plant of the genus Rheum. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a laxative that exhibits an effect of improving constipation in the intestine.

  The laxative according to the present invention contains anthraquinones extracted from the genus Rheum as an active ingredient, but the genus Rheum contains anthraquinones, and that anthraquinones have an effect of improving constipation (the axillary action) For example, it is described in Patent Documents 1 and 2, and the like.

  Patent Document 1 mentions Daihuang, a plant belonging to the genus Rheum, as a colonic stimulant laxative. In addition, an anthraquinone laxative extracted from Daihuang is listed as an armpit component.

  Patent document 2 relates to a laxative containing a herbal powder mixture and / or extract powder of Chinese medicine containing Daio, and a specific example of an anthraquinone laxative is a herbal laxative containing Daio as an active ingredient. ing.

  Daio is generally known as a rhizome herbal medicine. That is, in this kind of Rheum genus, only the underground part, that is, the rhizome part, is used as a herbal medicine component.

JP-A-8-310960 JP 2005-179316 A

  Despite the fact that Daiki's rhizome is one of the general-purpose herbal medicines, more than 95% of them are dependent on foreign countries. With regard to this kind of plant resources, there is a problem of rights represented by resource nationalism, and it is gradually becoming difficult to bring them out to other countries. For this reason, the demand for domestic cultivation has increased rapidly in recent years.

  The first example of cultivation of Daihuang in Japan is the cultivation of R. tanguticum by Professor Shoji Shibata of the University of Tokyo. Shinshu Daihuang currently cultivated in Hokkaido, using the former R. tanguticum seeds as material, repeated mating of R. palmumatum and R. coreanum since 1953 and spent more than 10 years It has been discovered. In addition, although there are reports of cultivation in Toyama Pharmaceutical University Medicinal Botanical Garden in Tateyama Yayogahara and Toga Village Itodajima, they have not reached the level of production cultivation.

  In this way, even if it is said to be domesticated, it takes a lot of time and research to reach production cultivation, such as selection of cultivated species, breeding, selection of suitable land for cultivation, and examination of breeding methods. It ’s not easy. In addition, in view of the fact that most of the great yellows in foreign countries are derived from wild products and the emergence of the previous resource nationalism, we cannot expect any future increase in supply from overseas.

  The present invention has been made to solve the above-described problems, and aims to stably supply an active ingredient related to a laxative derived from the genus Rheum and to effectively use the genus Rheum as a plant resource. As a result, the present invention aims to provide a novel laxative that can contribute to the protection of the genus Rheum.

  In the past, the inventors of the present invention have not made any investigation on the underground part of the genus Rheum, that is, only the rhizome part is used as a herbal medicine component and the medicinal use of the above-ground part, In many cases, the present invention has been completed as a result of intensive studies focusing on the fact that the above-ground part is disposed of.

  That is, the present invention is a laxative characterized by containing anthraquinones extracted from the above-ground part of the genus Rheum as an active ingredient.

  Specific examples of Rheum in the present invention include R.undulatum, R.officinale, R.palmatum, R.tanguticum and the like.

The anthraquinones in the present invention have a general formula represented by the following chemical formula.

  Specific examples of anthraquinones include emodin, Aloe-emodin, Chrysophanol, Aloe-emodin-1-O-β-D-glucopyranoside, Chrysophanol-1-O-β-D-glucopyranoside, Chrysophanol-8-O-β- And D-glucopyranoside and Chrysophanol-8-O-β-D- (G-galloyl) -glucopyranoside.

Among these anthraquinones, the most effective component is considered to be emodin represented by the following chemical formula 3.

In the present invention, anthraquinones were extracted from the aerial part instead of the rhizome part, which is the underground part of the Rheum genus which has been conventionally recognized as a herbal medicine component, and this was used as an active ingredient of a laxative. In the above-ground part of the genus Rheum, little research has been conducted on herbal ingredients, and many have been disposed of. Therefore, as in the present invention, if anthraquinones are extracted from the above-ground part of the genus Rheum and used as an active ingredient of the laxative, the whole from the underground part to the above-ground part of the genus Rheum can be used without waste. Since it can be used effectively, more active ingredients can be extracted from one Rheum genus.
This means that it is possible to contribute to the stable supply of anthraquinones derived from the genus Rheum, which is difficult to increase in imports from abroad and difficult to grow domestically. it can.

  There are various derivatives of anthraquinones, but in the present invention, emodin is considered to be an active ingredient of a laxative. As a document supporting such a majesty action of emodin, for example, “Effect of emodin on small intestinal peristalsis of mice and relevant mechanism: World journal of Gastroenterlogy: published on November 20, 2005” can be cited.

Hereinafter, the present invention will be described in detail with reference to experimental examples.
5 g of dried Rheum undulatum petiole was ground and extracted with 50 ml of 80% methanol overnight at room temperature. After filtration, 50 ml of 80% methanol was added to the residue again and mixed for 1 minute with an ortex mixer. The resulting mixture was filtered. The filtrates were combined and concentrated under reduced pressure to obtain an extract.
The extract was made up to 10 ml with 80% methanol and filtered through a 0.45 μm filter to obtain a sample solution for HPLC analysis.

FIG. 1 shows the results of HPLC analysis. The analysis conditions are as follows.
Column: Capcellpack C18AG-120A (5μm, 4.6mm id x 250mm, Shiseido)
Solvent: MeCN / H ₂ O (0.1% formic acid) gradient of 15% MeCN to 25% MeCN in 15 min to 70% MeCN in another 35 min, then to 100% MeCN in 10 min.
Flow rate: 0.9ml / min.
Column temp: 40 ℃
Detector: Shimadzu photodiode array SPDM-6A system (Shimadzu), UV: 275nm.

  From the HPLC pattern analysis results of FIG. 1, it can be seen that Rheum undulatum contains emodin, which can be an active ingredient of a laxative. Specifically, peak 1 in FIG. 1 shows cinnamic acid (cinnamic acid), peak 2 shows emodin (emodin), and it is confirmed from the peak position of peak 2 that Rheum undulatum contains emodin. We were able to. It is clear from the above-mentioned “Effect of emodin on small intestinal peristalsis of mice and relevant mechanism: World journal of Gastroenterlogy: issued November 20, 2005” and the like.

It is the analysis result of HPLC with respect to the extract of Daio.

Claims (2)

  A laxative comprising an anthraquinone extracted from the above-ground part of the genus Rheum as an active ingredient. The laxative according to claim 1, wherein the anthraquinones are emodin represented by the following chemical formula.

Images