WO2005099733A1

WO2005099733A1 - Vegetable antibacterial composition

Info

Publication number: WO2005099733A1
Application number: PCT/JP2004/013897
Authority: WO
Inventors: Katsuya Shimoo
Original assignee: Kobayashi Pharmaceutical Co., Ltd.
Priority date: 2004-03-31
Filing date: 2004-09-24
Publication date: 2005-10-27
Also published as: JP4666938B2; JP2005289912A

Abstract

[PROBLEMS] To provide an antibacterial agent having excellent antibacterial activity against bacterial of the Corynebacterium genus and provide a composition for external application containing relevant antibacterial composition. [MEANS FOR SOLVING PROBLEMS] There are provided an antibacterial agent against bacterial of the Corynebacterium genus, which comprises pieplant extract and/or soapberry extract as an active ingredient, and provided a composition for external application comprising the antibacterial agent.

Description

Plant antibacterial composition

Technical field

The present invention relates to an antibacterial agent against Corynebacterium spp. Containing a crude drug extract as an active ingredient, and an external composition containing the antibacterial agent.

Background art

[0002] It is known that the unpleasant odor associated with perspiration is caused by the decomposition of naturally odorless and sterile sweat by microorganisms on the skin surface, and when ammonia in the skin surface and sweat is added to this, It also gives a unique odor. Axillary odor, which is a unique odor that causes a nose among body odors, is particularly the apotaxin sweat gland force in the axillary area.Apoptalin sweat is secreted on the skin surface. A collection of bacteria that are retained and settled on the skin is called the skin flora, and is generated by being decomposed (for example, see Non-Patent Document 1).

Among these skin flora, the microorganism directly related to armpit odor is known to be Corynebacterium sp.

[0003] Conventionally, antiperspirants, antiperspirant astringents, antibiotics or antibacterial agents and the like have been used to suppress the generation of body odor. However, antiperspirants and antiperspirants are not very effective for those who secrete a large amount of sweat. In particular, antiperspirants that also have aluminum salt power, such as hydroxychloride aluminum (aluminium chloride hydrate). Has a problem that it is highly irritating to the skin due to its protein denaturing action. In addition, antibacterial agents and antibiotics, such as Shii-Dani Benzetonium, are effective, but not only microorganisms that cause body odor but also those that form skin flora. Because of sterilization or bacteriostasis, there is a risk that the skin's original self-cleansing action is impaired due to the formation of healthy skin flora.

[0004] Therefore, in order to develop a method for preventing unpleasant body odor, it is necessary to find an antibacterial agent having a bactericidal action that is selective for bacteria that cause unpleasant odor. Non-Patent Document 1: Hurley, HJJr .: Diseases of apocrinesweat glands.Dermatology in General Medecine.p.386, McGraw—Hill'New York (1971)

Disclosure of the invention

Problems to be solved by the invention

[0005] It is a main object of the present invention to provide an antibacterial agent having an excellent antibacterial activity against Corynebacterium, and to provide an external composition containing the antibacterial agent. Means for solving the problem

[0006] The present inventors have found that the extract has a superior antibacterial action against Corynebacterium spp. And has completed the present invention.

[0007] The present invention provides the following antibacterial agents and external compositions containing the antibacterial agents.

Item 1. An antibacterial agent against Corynebacterium, comprising at least one active ingredient, Daio Extract or Muclojex.

Item 2. The antibacterial agent according to Item 1, which is Corynebacterium xylosis, Corynebacterium minutissimam or Corynebacterium tenuis.

Item 3. The antibacterial agent according to Item 1 or 2, further containing methyl paraoxybenzoate. Item 4. An external composition containing the antibacterial agent according to any one of Items 1-3.

Item 5. The externally applied composition according to Item 4, which is for preventing axillary odor.

[0008] The dio extract, which is an active ingredient of the antibacterial agent of the present invention, is usually a dino extract of Polygonaceae.

(Ohio) that can also obtain rhizome power is used. In addition, as the mukurojekis, one obtained from the skin of the mukuro mushroom, which is a member of the family Scrophulariaceae, is usually used. These herbal extracts can be obtained from the whole plant (whole plant) or any part (e.g., leaves, roots, stems, fruits, bark, etc.) as long as they have an antibacterial effect against Corynebacterium spp. Use the extracted ones.

As a method for producing the dio extract and muclojex used in the present invention, conventionally known methods can be used. For example, the above-mentioned dried plant is extracted with water or hot water, or extracted with an organic solvent. And a method of filtration. The extraction conditions are not particularly limited as long as they are generally used for plant extraction. After extraction and filtration, the extract may be concentrated if necessary. As a method of concentration, evaporator or the like Conventional methods can be used.

[0010] As a solvent used for the extraction, a known solvent may be used. For example, water, methanol, ethanol, propanol, isopropanol, butanol, ethylene glycol, propylene glycol, 1,3- Organic solvents such as butylene glycol, diethylene glycol, dipropylene glycol, isoprene glycol, hexylene glycol, glycerin, methyl acetate, ethyl acetate, isopropyl acetate, ethyl ether, isopropyl ether, and acetone can be used, and water and ethanol are preferred. From these extraction solvents, one type may be used alone, or two or more types may be used in combination.

[0011] The extract thus obtained can be used as it is as the antibacterial agent of the present invention, or can be subjected to purification operations such as deodorization and decolorization within a range not to lose the effects of the present invention. it can.

Further, the antibacterial agent of the present invention can be used in the form of a powder by evaporating and drying the extraction solvent from the above extract, or using a solvent such as water, ethanol, propylene glycol, 1,3-butylendalcol, glycerin and the like. Can also be used after redissolving it.

[0012] The antibacterial agent of the present invention is characterized by having a bactericidal action against Corynebacterium sp. Here, the genus Corynebacterium is not particularly limited as long as it is a gram-positive bacterium within the genus of the genus, but is preferably a bacterium that causes the generation of axillary odor. There are known Terinium minuteisium (oryneoacterium minutissimum), Corynebacterium xerosis, and Corynebacterium tenuis.

[0013] If the extract contains a component extracted from a dry weight of 6 g of "Dio" described in the Pharmacopoeia, the effective amount of the Dio extract in the antibacterial agent of the present invention is used. Is, for example, about 1% by weight or more, preferably about 5% by weight or more, based on the total amount of the agent.

[0014] If the extract lg contains an extract component extracted from 10 g of dry weight of mud roe (pericarp) as mukuro gekikiss, the effective amount of mud roe extract in the antibacterial agent of the present invention is as follows: For example, about 1% by weight or more, preferably 5% by weight, based on the total amount of the agent. The weight percentage is, for example, about or more.

[0015] As the antibacterial agent of the present invention, Daio extract or muclogekis may be used alone or in combination. The mixing ratio when two extracts are used in combination is not particularly limited as long as the effect as the antibacterial agent of the present invention can be obtained, and those skilled in the art can easily determine the effective amount with reference to the above effective amount. Can be.

[0016] The antibacterial agent of the present invention can also contain other antibacterial components as components other than the dio extract or the mukuro sage extract as long as the effects of the present invention are not impaired. Specific examples of such antibacterial components include triclosan, trichlorocarbalide, Shirazari benzalco-pam, Shiridani benzet-pam, halocarban, chlorhexidine hydrochloride, dihydropharmanesol, and isopropanol. Examples include pyrmethylphenol, thymol, hinokitiol, cetylpyridyl-dimethyl chloride, ethanol, and isopropanol. When these antimicrobial components are added to the antimicrobial agent of the present invention, an antimicrobial effect can be obtained with a smaller amount than the above. Therefore, the effective amount of Dio extract or muclojekis in the antibacterial agent of the present invention is not limited to the above-mentioned range (1% by weight or more as Dio extract or muclojekis based on the total amount of the antibacterial agent).

[0017] As shown in Example 1, the antibacterial effect of the present invention is further enhanced by using parabens, which are conventionally known as preservatives, in combination with the antibacterial agent of the present invention. Examples of the parabens include methyl noraoxybenzoate, ethyl ethyl paraoxybenzoate, propyl noraoxybenzoate and the like. Particularly, it is preferable to use methyl paraoxybenzoate in combination.

When methyl noraoxybenzoate is used, the amount of the norabenes is about 0.01% by weight or more, preferably about 0.05% by weight or more, more preferably about 0.1% by weight or less. Above.

The antibacterial agent of the present invention has an excellent antibacterial action against Corynebacterium spp. Present on the skin, and thus can be used as an antibacterial component in an external composition. Since Corynebacterium is a causative bacterium of axillary odor, the antibacterial agent of the present invention can be used as an antibacterial component of an external composition for preventing axillary odor.

[0020] In this case, the mixing ratio of the antibacterial agent of the present invention to the external composition is determined according to the active ingredient ゥ Extract or muclogekis may be used in an amount that exerts an antibacterial effect on Corynebacterium spp.For example, as dio extract or muclojekis contained in an antibacterial agent

The amount is, for example, about 1% by weight or more, preferably about 5% by weight or more, based on the total amount of the agent.

[0021] The present invention also provides an external composition which is prepared by force. The composition for external use can be widely used in the fields of medicines, quasi-drugs, and cosmetics for the purpose of preventing axillary odor. Creams, ointments, lotions, sprays, aerosols, emulsions, foams, detergents, stones, bath agents, skin conditioners, lotions, knocking agents, foundations, and antibacterial agents are not particularly limited. The liquid, semi-solid, foam, powder, and solid forms are appropriately prepared in accordance with the usage form of a sheet, patch, cataplasm, roll-on agent, or the like in which the agent is impregnated in a sheet-like base material.

[0022] The composition for external use of the present invention is prepared by adding dioxin and Z or deer extract extract to a carrier that is generally blended with the composition for external use according to various forms; a preservative, a preservative, and a stabilizer , Suspending agents, pH adjusters, fragrances, surfactants (aionic, cationic, nonionic, amphoteric surfactants), coloring agents, fining agents, viscosity modifiers, bactericidal antibacterial agents, essential oils, etc. Various additives can be blended. Carriers and additives that are safe for the human body are preferred, and those that are not irritating to the skin and the like are preferred.

[0023] Examples of the carrier include water (for example, ion-exchanged water), alcohol (for example, ethanol) and a water-soluble solvent (for example, propylene glycol and glycerin) in the case of a liquid form, and various kinds of in the case of a solid form. Gelling agents (for example, seaweed polysaccharides such as carrageenan and sodium alginate; seed polysaccharides such as guar gum and tamarind seed gum; plant liquid polysaccharides such as arabia gum and tragacanth gum; xanthan gum; Proteins such as gelatin; cellulose derivatives such as carboxymethylcellulose; inorganic materials such as talc and silica; and polyacrylic acids such as sodium polyacrylate.

[0024] Preservatives include benzoic acid, sorbic acid, propionic acid, dehydroacetic acid, salicylic acid, edetic acid and salts thereof; inorganic salts such as sodium sulfite, sodium hyposulfite, sodium pyrosulfite; plants such as pectin extract; Derived components and the like can be exemplified. [0025] Examples of the precipitation inhibitor include fine particles such as silicon dioxide and magnesium stearate, surfactants such as polyoxyethylene hydrogenated castor oil and polyoxyethylene sorbitan monostearate, and gels such as xanthan gum and polyethylene glycol. Agents and the like can be exemplified.

[0026] Examples of the pH adjuster include citric acid, fumaric acid, DL-malic acid and salts thereof; carbonates such as sodium hydrogen carbonate and potassium carbonate; phosphates such as phosphoric acid and sodium dihydrogen phosphate. Can be listed.

[0027] The various components described above are merely examples, and the present invention is not limited to these components. That is, in the antimicrobial agent of the present invention, various components usually used as components of the external preparation can be used, including other additional components.

[0028] The antibacterial agent of the present invention has a bactericidal action against Corynebacterium spp., Which produces an unpleasant odor source by decomposing sweat and sebum secretions, and is therefore useful for preventing body odors such as axillary odor and sweat odor. is there.

[0029] The external composition of the present invention, particularly an external composition for preventing axillary odor, has an axillary odor and sweat odor based on a bactericidal action against Corynepacterimus spp., Especially an axillary odor bacterium which causes the generation of axillary odor. Excellent in preventing the occurrence. Accordingly, the composition for external use can be positioned as a deodorant (body deodorant), particularly a deodorant odorant, used for suppressing the generation of axillary odor and sweat odor.

Further, the antibacterial agent of the present invention is prepared in the form of the above-mentioned composition for external use, and is applied directly to the skin (skin surface) on which Corynebacterium spp. Is present, as well as in the form of spray or aerosol. Spraying, dipping, or coating on clothing such as underwear or shirt to which Corynebacterium or Staphylococcus epidermidis has adhered (or may adhere) by preparing it in the form of a laundry finish or coating agent It can be used in any usage mode.

The deodorant of the present invention (body deodorant and axillary deodorant) may be added to the above-mentioned other antibacterial components within a range not to impair the effects of the present invention, and may be used as a spray or aerosol for clothing. Known preservatives, preservatives, stabilizers, sedimentation inhibitors, pH adjusters, fragrances, surfactants (aionic, cationic, nonionic, amphoteric surfactants) Clarifying agent, viscosity adjustment And various additives such as an antimicrobial agent and a bactericidal antibacterial agent. Such carriers and additives are preferably those that are safe for the human body, more preferably those that are not irritating to the skin and the like.

The invention's effect

According to the present invention, it is possible to provide an antibacterial agent having an excellent antibacterial activity against Corynebacterium spp., And an external composition containing the antibacterial agent. In addition, since the antibacterial agent of the present invention contains a crude drug extract as an active ingredient, it can provide an antibacterial agent having low irritation to the skin and an external composition for preventing axillary odor.

BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the present invention will be specifically described with reference to Examples and Formulation Examples, but the present invention is not limited to these Examples.

Example 1

[0034] Test method

The antibacterial activity was evaluated by measuring the blocking circle diameter by the paper disk method.

[0035] Corynebacterium minutismimum was uniformly applied to a trypticase soy agar medium in a Petri dish with a conical rod, and the cells were enriched at 35 ° C for 24 hours. Macfaland O.5 was suspended in saline.

[0036] On a trypticase soy agar medium in a petri dish, 50 µL of this suspension is evenly applied with a conical rod, and a paper disc (diameter 8 mm) is placed on top of the suspension to prepare a sample (Diowex or Mukurojekis). 50 L was impregnated. After incubation at 35 ° C for 24 hours, the presence or absence of the inhibition circle and its diameter were measured.

[0037] Test results

Inhibition circle formation was observed even with Daio extract or muclogekis alone, but the antibacterial effect was further enhanced by adding methyl nonoxybenzoate to each extract.

[0038] Table 1 shows the measurement results by the paper disk method.

[Table 1] Comparative example 1 Comparative example 2 Experimental example 1 Experimental example 2 Experimental example 3 Experimental example 4 Experimental example 5 Experimental example 6 Experimental example 7 Experimental example 8 Daio extract [% by weight] 0.0 0.0 5.0 10.0 0.0 0.0 5.0 10.0 0.0 0.0 Muclojekis [Weight <¼ ] 0.0 0.0 0.0 0.0 5.0 10.0 0.0 0.0 5.0 10.0 Methyl paraoxybenzoate [wt%] 0.1 0.2 0.0 0.0 0.0 0.0 0.1 0.2 0.1 0.2 Purified water [wt%] 99.9 99.8 95.0 90.0 95.0 90.0 94.9 89.8 94.9 89.8 Blocking circle diameter [mm ]-12 10 13 12 13 14 16 15 21

Example 2

Hereinafter, formulation examples of the preparation using Daio extract are shown, but the present invention is not limited to these. In each of the formulation examples, Daio extract can be changed to muclogekis. In addition, it is also possible to further add 0.2% by weight of methyl paraoxybenzoate to Formulation Examples 1 and 3. In that case, the total amount is adjusted according to the amount of purified water.

Daio Extract

Dipotassium glycyrrhizinate

Bentonite

Kaolin

Glycerin

Propylene glycol Total 100

[Prescription example 2]

Liquid [g]

Dai Sai Extract 5

Polyoxyethylene oleyl ether 1

Ethanore 10

Methyl paraoxybenzoate 0.2

Purified ice 83J_

Total 100

[Prescription example 3]

Aerosol [g]

Dai Sai Extract 5

Isopropyl methylphenol 0.1

Ethanol 20 Purified water 24.9 Liquefied petroleum gas 50

Claims

The scope of the claims

[1] An antibacterial agent against Corynebacterium spp., Comprising at least one of Daio Extract or Muclogekis as an active ingredient.

[2] The antibacterial agent according to claim 1, which is Corynebacterium xylosis, Corynebacterium xylosis, Corynebacterium minuteishimam or Corynebacterium tenuis.

[3] The antibacterial agent according to claim 1 or 2, further comprising methyl paraoxybenzoate.

[4] An external composition containing the antibacterial agent according to any one of [13] to [13].

[5] The composition for external use according to claim 4, which is for preventing axillary odor.