JP2008156272A - Cosmetic - Google Patents
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- JP2008156272A JP2008156272A JP2006346552A JP2006346552A JP2008156272A JP 2008156272 A JP2008156272 A JP 2008156272A JP 2006346552 A JP2006346552 A JP 2006346552A JP 2006346552 A JP2006346552 A JP 2006346552A JP 2008156272 A JP2008156272 A JP 2008156272A
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- 239000002537 cosmetic Substances 0.000 title claims abstract description 17
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000012528 membrane Substances 0.000 claims abstract description 47
- 229960001340 histamine Drugs 0.000 claims abstract description 27
- 108091005804 Peptidases Proteins 0.000 claims abstract description 21
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 10
- 239000004615 ingredient Substances 0.000 claims abstract description 5
- 102000035195 Peptidases Human genes 0.000 claims description 16
- 210000001672 ovary Anatomy 0.000 claims description 15
- 241000255925 Diptera Species 0.000 claims description 9
- 230000002611 ovarian Effects 0.000 abstract description 36
- 241000972773 Aulopiformes Species 0.000 abstract description 5
- 239000004365 Protease Substances 0.000 abstract description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 5
- 235000019515 salmon Nutrition 0.000 abstract description 5
- 208000003251 Pruritus Diseases 0.000 abstract description 3
- 230000000172 allergic effect Effects 0.000 abstract description 3
- 208000010668 atopic eczema Diseases 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 208000024891 symptom Diseases 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 13
- 239000000706 filtrate Substances 0.000 description 12
- 241000251468 Actinopterygii Species 0.000 description 9
- 235000019688 fish Nutrition 0.000 description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- 230000005764 inhibitory process Effects 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000007864 aqueous solution Substances 0.000 description 6
- 241000723298 Dicentrarchus labrax Species 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 239000003163 gonadal steroid hormone Substances 0.000 description 4
- 210000003630 histaminocyte Anatomy 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000283690 Bos taurus Species 0.000 description 3
- -1 etc. Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- 241000252203 Clupea harengus Species 0.000 description 2
- 241000276457 Gadidae Species 0.000 description 2
- 206010000496 acne Diseases 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 238000005238 degreasing Methods 0.000 description 2
- 238000004332 deodorization Methods 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 235000019514 herring Nutrition 0.000 description 2
- 230000007803 itching Effects 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 238000000409 membrane extraction Methods 0.000 description 2
- 230000000051 modifying effect Effects 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000277269 Oncorhynchus masou Species 0.000 description 1
- 241000277275 Oncorhynchus mykiss Species 0.000 description 1
- 241000277277 Oncorhynchus nerka Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000003788 bath preparation Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 229920002055 compound 48/80 Polymers 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 239000005555 hypertensive agent Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- ZWLUXSQADUDCSB-UHFFFAOYSA-N phthalaldehyde Chemical compound O=CC1=CC=CC=C1C=O ZWLUXSQADUDCSB-UHFFFAOYSA-N 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000002374 sebum Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 239000006097 ultraviolet radiation absorber Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Landscapes
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
本発明は、鮭類の卵巣膜または該卵巣膜から抽出された成分を含む化粧料に関するものである。 The present invention relates to a cosmetic comprising a mosquito ovary membrane or a component extracted from the ovarian membrane.
従来、魚類の卵巣膜(魚卵外皮)を予めオゾン水で処理した後、その構成タンパク質である筋原線維タンパク質を酵素分解してアミノ酸及びペプチドを抽出する方法が知られている(例えば特許文献1参照)。 Conventionally, a method of extracting amino acids and peptides by treating the ovarian membrane (fish egg rind) of fish with ozone water in advance and then enzymatically degrading myofibrillar protein, which is a constituent protein thereof, is known (for example, patent document). 1).
前記アミノ酸及びペプチドは、生理活性物質として、あるいは食品強化剤として用いることができるとされており、さらに詳しくは、ACE阻害活性を備え、血圧上昇抑制剤(降圧剤)として作用するとされている。 The amino acids and peptides can be used as physiologically active substances or as food fortifiers. More specifically, the amino acids and peptides have ACE inhibitory activity and act as blood pressure increase inhibitors (hypertensive agents).
また、マダラ科、タラ科、ニシン科の魚類の卵巣膜をタンパク分解酵素であるプロテアーゼで処理して得られた成分を化粧品製剤として用いることが知られている(例えば特許文献2参照)。 In addition, it is known to use a component obtained by treating the ovarian membrane of a fish of the cod family, cod family, and herring family with a protease that is a proteolytic enzyme as a cosmetic preparation (see, for example, Patent Document 2).
前記マダラ科、タラ科、ニシン科の魚類の卵巣膜をプロテアーゼで処理して得られた成分は、性ホルモンの作用の活性化及び抑制を行い、性ホルモンのバランスを正常化する作用(性ホルモン修飾作用)を有するとされている。そして、前記化粧品製剤は、前記成分の性ホルモン修飾作用により、皮脂の分泌を抑制し、ニキビ、吹き出物、肌荒れ、しわ等の改善に効果があるとされている。 The component obtained by treating the ovarian membrane of the codfish family, codfish family, and herring family fish with a protease activates and inhibits the action of sex hormones and normalizes the sex hormone balance (sex hormones) It is said to have a modifying action. The cosmetic preparation is said to be effective in improving acne, pimples, rough skin, wrinkles and the like by suppressing the secretion of sebum by the sex hormone modifying action of the components.
しかしながら、魚類の卵巣膜から抽出された成分には、前記血圧の降圧作用、性ホルモン修飾作用以外の作用は知られておらず、さらに多くの用途の開発が望まれる。
本発明は、かかる事情に鑑み、鮭類の卵巣膜自体または該卵巣膜から抽出された成分の新たな用途を提供することを目的とする。 In view of such circumstances, an object of the present invention is to provide a new use of a mosquito ovary membrane itself or a component extracted from the ovarian membrane.
かかる目的を達成するために、本発明の化粧料は、鮭類の卵巣膜自体または該卵巣膜をタンパク分解酵素で処理することにより抽出された成分を含み、ヒスタミン遊離抑制作用を備えることを特徴とする。 In order to achieve this object, the cosmetic of the present invention comprises a mosquito ovary membrane itself or a component extracted by treating the ovarian membrane with a proteolytic enzyme, and has a histamine release inhibitory action. And
本発明の化粧料によれば、鮭類の卵巣膜自体または該卵巣膜をタンパク分解酵素で処理することにより抽出された成分を含むことにより、ヒスタミン遊離抑制作用が得られる。従って、本発明の化粧料によれば、ヒスタミンによる皮膚の発赤、かゆみ等のアレルギー症状を抑制する効果を得ることができる。 According to the cosmetics of the present invention, the histamine release inhibitory action can be obtained by including the ovarian membrane itself of moss or the component extracted by treating the ovarian membrane with a proteolytic enzyme. Therefore, according to the cosmetic of the present invention, it is possible to obtain the effect of suppressing allergic symptoms such as skin redness and itching caused by histamine.
前記タンパク分解酵素は、鮭の卵巣膜を構成するタンパク質を分解できるものであればどのようなものであってもよい。 The proteolytic enzyme may be any one as long as it can degrade the protein that constitutes the ovarian membrane of rabbits.
次に、本発明の実施の形態についてさらに詳しく説明する。 Next, embodiments of the present invention will be described in more detail.
本実施形態の化粧料は、鮭類の卵巣膜自体または該卵巣膜をタンパク分解酵素で処理することにより抽出された成分を含むものである。 The cosmetic of the present embodiment contains a mosquito ovary membrane itself or a component extracted by treating the ovarian membrane with a proteolytic enzyme.
前記鮭類に属する魚類としては、白ザケ、紅ザケ、銀ザケ、ニジマス、サクラマス、マスノスケ等を挙げることができる。前記鮭類の卵巣膜は、前記鮭類に属する魚類の魚卵外皮であり、卵巣から魚卵を採取した後の外皮のみを水洗することにより得ることができる。本実施形態の化粧料は、前記水洗後、前記鮭類の卵巣膜をミキサー、乳鉢、乳化機等で粉砕した物をそのまま用いてもよく、前記卵巣膜をタンパク分解酵素で処理することにより抽出された成分を用いてもよい。 Examples of the fish belonging to the moss include white salmon, red salmon, silver salmon, rainbow trout, cherry salmon, and salmon. The ovary membrane of the moss is a fish egg rind of fish belonging to the moss, and can be obtained by washing only the rind after collecting the egg from the ovary. The cosmetic of this embodiment may be used as it is after washing with water, and the ovarian membrane of the moss crushed with a mixer, a mortar, an emulsifier or the like, and extracted by treating the ovarian membrane with a protease. Ingredients may be used.
前記卵巣膜をタンパク分解酵素で処理することにより抽出された成分(以下、卵巣膜抽出成分と略記する)を用いる場合、卵巣膜抽出成分は、例えば、次のようにして調製することができる。 When using a component extracted by treating the ovarian membrane with a proteolytic enzyme (hereinafter abbreviated as an ovarian membrane extraction component), the ovarian membrane extraction component can be prepared, for example, as follows.
まず、前記のようにして得られた鮭類の卵巣膜を原料とし、該卵巣膜に対して水を卵巣膜:水=1:1〜1:3の重量比で加えて撹拌、混合し、さらにタンパク分解酵素を卵巣膜の全量に対して1〜3重量%の範囲で添加し、45〜55℃の温度で30分間〜5時間、好ましくは2時間加熱する。このようにすると、前記卵巣膜の成分のうち、前記タンパク分解酵素で分解された成分が水中に溶出し、該成分の水溶液が得られる。 First, using the mosquito ovary membrane obtained as described above as a raw material, water is added to the ovarian membrane in a weight ratio of ovarian membrane: water = 1: 1 to 1: 3, and the mixture is stirred and mixed. Furthermore, proteolytic enzyme is added in the range of 1 to 3% by weight with respect to the total amount of the ovarian membrane, and heated at a temperature of 45 to 55 ° C. for 30 minutes to 5 hours, preferably 2 hours. If it does in this way, the component decomposed | disassembled with the said proteolytic enzyme will elute in water among the components of the said ovary membrane, and the aqueous solution of this component will be obtained.
次に、前記水溶液に含まれている前記タンパク分解酵素を失活する。前記失活は、例えば、前記水溶液を90℃の温度で5分間加熱することにより行うことができる。 Next, the proteolytic enzyme contained in the aqueous solution is inactivated. The deactivation can be performed, for example, by heating the aqueous solution at a temperature of 90 ° C. for 5 minutes.
次に、前記水溶液を30メッシュ程度の金網で簡易濾過し、未分解の卵巣膜等の粗大物を除去する。そして、得られた濾液に活性炭を添加して、該濾液の脱臭、脱色、脱脂を行う。前記濾液の脱臭、脱色、脱脂は、前記原料としての卵巣膜の全量に対して2〜4重量%の範囲の活性炭を該濾液に添加し、例えば60℃の温度で30分間加熱することにより行うことができる。 Next, the aqueous solution is simply filtered through a wire mesh of about 30 mesh to remove coarse materials such as undecomposed ovarian membranes. Then, activated carbon is added to the obtained filtrate to deodorize, decolorize, and degrease the filtrate. The deodorization, decolorization, and degreasing of the filtrate is performed by adding activated carbon in the range of 2 to 4% by weight to the total amount of the ovarian membrane as the raw material and heating the filtrate at a temperature of 60 ° C. for 30 minutes, for example. be able to.
前記活性炭による脱臭、脱色、脱脂処理後、前記濾液を例えばフィルタープレスにより濾過し、得られた濾液を、減圧下、例えば60℃の温度で濃縮した後、例えば80℃の温度に10分間維持して滅菌する。そして、滅菌後の前記濾液をスプレードライにて乾燥させることにより、前記卵巣膜抽出成分を得ることができる。前記卵巣膜抽出成分は、アミノ酸、ペプチド、ビタミン、ミネラル、糖類、酵素、核酸及びその代謝物、各種成長因子、サイトカイン等を含んでいる。 After the deodorization, decolorization, and degreasing treatment with the activated carbon, the filtrate is filtered by, for example, a filter press, and the obtained filtrate is concentrated at a temperature of 60 ° C. under reduced pressure, for example, and then maintained at a temperature of 80 ° C. for 10 minutes, for example. And sterilize. Then, the ovarian membrane extract component can be obtained by drying the sterilized filtrate by spray drying. The ovarian membrane extract component contains amino acids, peptides, vitamins, minerals, sugars, enzymes, nucleic acids and their metabolites, various growth factors, cytokines, and the like.
本実施形態の化粧料は、前記鮭類の卵巣膜自体または該卵巣膜をタンパク分解酵素で処理することにより抽出された成分に、常法に従って油分、界面活性化剤、ビタミン剤、紫外線吸収剤、増粘剤、保湿剤、副素材、防腐剤、着色料等を所望に従って添加することにより調製することができる。化粧料の形態は任意であり、溶液状、クリーム状、ペースト状、ゲル状、ジェル状、気泡状、固形状、顆粒状、粉末状等として用いることができ、化粧水、クリーム、軟膏、ローション、乳液、パック、シート、オイル、石鹸、洗顔料、香料、オーディコロン、浴用剤、シャンプー、リンス等に用いることができる。 The cosmetic composition of the present embodiment includes an oil, a surfactant, a vitamin agent, and an ultraviolet absorber according to a conventional method for the ovarian membrane itself of the moss or a component extracted by treating the ovarian membrane with a proteolytic enzyme. It can be prepared by adding thickeners, humectants, auxiliary materials, preservatives, colorants and the like as desired. The form of the cosmetic is arbitrary, and can be used as a solution, cream, paste, gel, gel, foam, solid, granule, powder, etc., lotion, cream, ointment, lotion , Emulsions, packs, sheets, oils, soaps, facial cleansers, fragrances, audio colons, bath preparations, shampoos, rinses and the like.
次に、本発明の実施例を示す。 Next, examples of the present invention will be described.
北海道産白ザケの卵巣から魚卵を採取した後の卵巣膜をよく水洗したもの100gに、精製水100mlを加え、ミキサー(松下電器産業株式会社製、商品名:MX−X107)を用いて、1分間粉砕する操作を10回行い、粉砕物を得た。次に、前記粉砕物をさらに乳鉢ですり潰し、300メッシュの金網で濾過したものを試料とした。 100 g of purified ovarian membrane after collecting fish eggs from ovary of white salmon from Hokkaido, 100 ml of purified water added, and using a mixer (trade name: MX-X107, manufactured by Matsushita Electric Industrial Co., Ltd.) The operation of pulverizing for 1 minute was performed 10 times to obtain a pulverized product. Next, the pulverized product was further ground with a mortar and filtered through a 300-mesh wire mesh.
本実施例で得られた試料は、白ザケの卵巣膜の粉砕物である。次に、本実施例で得られた試料を用いてヒスタミン遊離抑制試験を行った。 The sample obtained in this example is a ground product of white salmon ovarian membrane. Next, a histamine release inhibition test was performed using the sample obtained in this example.
前記ヒスタミン遊離抑制試験では、まず、前記試料を精製水を用いて固形分0.2%となるように稀釈し、試料溶液を調製した。 In the histamine release inhibition test, first, the sample was diluted with purified water to a solid content of 0.2% to prepare a sample solution.
次に、ラット(Slc:Wister系雄性ラット、約4〜9週齢)の腹腔内から採取した肥満細胞浮遊液1.2mlに、前記試料溶液0.2mlと、ヒスタミン遊離剤(シグマ・アルドリッチ社製、商品名:コンパウンド48/80)とを加え、試料の濃度が1μg/mlになるようにして混合液を調製した。 Next, 1.2 ml of the mast cell suspension collected from the abdominal cavity of a rat (Slc: Wistar male rat, about 4 to 9 weeks old) was added to 0.2 ml of the sample solution and a histamine releaser (Sigma Aldrich). And a trade name: Compound 48/80) was added to prepare a mixed solution so that the concentration of the sample was 1 μg / ml.
次に、前記混合液を37℃で15分間培養した後、氷冷して反応を停止し、反応液を遠心分離して、遊離したヒスタミンを上澄から抽出、精製した。次いで、精製したヒスタミンをo−フタルジアルデヒドにて発色させ、励起波長360nm、蛍光波長450nmにおける蛍光吸光度を測定し、次式によりヒスタミン遊離抑制率を求めた。 Next, the mixture was incubated at 37 ° C. for 15 minutes, and then ice-cooled to stop the reaction. The reaction solution was centrifuged, and the released histamine was extracted from the supernatant and purified. Subsequently, the purified histamine was developed with o-phthaldialdehyde, the fluorescence absorbance at an excitation wavelength of 360 nm and a fluorescence wavelength of 450 nm was measured, and the histamine release inhibition rate was determined by the following formula.
ヒスタミン遊離抑制率(%)={1−(A−C)/(B−C)}×100
但し、式中、Aは肥満細胞に試料を共存させてヒスタミン遊離剤を添加したときに遊離したヒスタミンの蛍光強度、Bは肥満細胞にヒスタミン遊離剤を添加したときに遊離したヒスタミンの蛍光強度、Cは肥満細胞から自然に遊離されるヒスタミンの蛍光強度を示し、A,B,Cはいずれも測定値から盲検値を差し引いたものである。
Histamine release inhibition rate (%) = {1− (A−C) / (B−C)} × 100
In the formula, A is the fluorescence intensity of histamine released when a sample was coexisted with mast cells and a histamine releaser was added, and B was the fluorescence intensity of histamine released when a histamine releaser was added to mast cells, C shows the fluorescence intensity of histamine naturally released from mast cells, and A, B, and C are obtained by subtracting the blinded value from the measured value.
結果を表1に示す。 The results are shown in Table 1.
実施例1で得られた試料に、精製水300mlを加え、高圧乳化機(みづほ工業株式会社製、商品名:マイクロフルイダイザーM110−E/H)を用いて、処理圧150MPaで粉砕する操作を2回行い、試料を得た。 An operation of adding 300 ml of purified water to the sample obtained in Example 1 and pulverizing it at a processing pressure of 150 MPa using a high-pressure emulsifier (trade name: Microfluidizer M110-E / H, manufactured by Mizuho Kogyo Co., Ltd.). Performed twice to obtain a sample.
本実施例で得られた試料は、白ザケの卵巣膜の微粉砕物である。次に、本実施例で得られた試料を用いた以外は、実施例1と全く同一にして、ヒスタミン遊離抑制試験を行い、ヒスタミン遊離抑制率を求めた。結果を表1に示す。 The sample obtained in this example is a finely pulverized ovarian membrane of white salmon. Next, a histamine release inhibition test was performed in the same manner as in Example 1 except that the sample obtained in this example was used, and the histamine release inhibition rate was obtained. The results are shown in Table 1.
実施例2で得られた試料(水溶液)のpHを7.2〜7.5に調整したのち、タンパク分解酵素としてアクチナーゼE(科研製薬株式会社製)を0.5g添加し、40℃で3時間、撹拌しながら反応させた。 After adjusting the pH of the sample (aqueous solution) obtained in Example 2 to 7.2 to 7.5, 0.5 g of actinase E (manufactured by Kaken Pharmaceutical Co., Ltd.) was added as a proteolytic enzyme, The reaction was allowed to stir for an hour.
次に、前記水溶液に含まれている前記タンパク分解酵素を、90℃の温度で5分間加熱して、前記タンパク分解酵素を失活させた後、30メッシュのフィルターで簡易濾過し、未分解の卵巣膜等の粗大物を除去した。 Next, the proteolytic enzyme contained in the aqueous solution is heated at a temperature of 90 ° C. for 5 minutes to inactivate the proteolytic enzyme, and then simply filtered through a 30-mesh filter. Coarse material such as ovarian membrane was removed.
次に、前記簡易濾過で得られた濾液に活性炭500gを添加し、60℃の温度で30分間加熱して、該濾液の脱脂、脱色、脱臭を行った後、前記濾液をフィルタープレスにより濾過し、得られた濾液を、減圧下、濃縮し、滅菌した。そして、滅菌後の前記濾液をスプレードライにて乾燥させることにより、試料を得た。 Next, 500 g of activated carbon is added to the filtrate obtained by the simple filtration, and the filtrate is degreased, decolored and deodorized by heating at a temperature of 60 ° C. for 30 minutes, and then the filtrate is filtered with a filter press. The filtrate obtained was concentrated and sterilized under reduced pressure. The sterilized filtrate was dried by spray drying to obtain a sample.
本実施例で得られた試料は、白ザケの卵巣膜抽出成分である。次に、本実施例で得られた試料を用いた以外は、実施例1と全く同一にして、ヒスタミン遊離抑制試験を行い、ヒスタミン遊離抑制率を求めた。結果を表1に示す。 The sample obtained in the present example is an ovarian membrane extract component of white salmon. Next, a histamine release inhibition test was performed in the same manner as in Example 1 except that the sample obtained in this example was used, and the histamine release inhibition rate was obtained. The results are shown in Table 1.
Claims (2)
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EP07024856A EP1938866B1 (en) | 2006-12-22 | 2007-12-20 | Cosmetic product comprising a component extracted from a Salmonidae fish ovarian membrane |
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AT07024856T ATE525114T1 (en) | 2006-12-22 | 2007-12-20 | COSMETIC PRODUCT CONTAINING A SALMONIDAE OVARIAN MEMBRANE INGREDIENT |
US11/963,756 US20080152683A1 (en) | 2006-12-22 | 2007-12-21 | Cosmetic Product |
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