CN101204363B - Cosmetic product - Google Patents
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- CN101204363B CN101204363B CN2007103020647A CN200710302064A CN101204363B CN 101204363 B CN101204363 B CN 101204363B CN 2007103020647 A CN2007103020647 A CN 2007103020647A CN 200710302064 A CN200710302064 A CN 200710302064A CN 101204363 B CN101204363 B CN 101204363B
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Abstract
A new application of a component extracted from a Salmonidae fish ovarian membrane is provided. A cosmetic product of the present invention contains a Salmonidae fish ovarian membrane or a component obtained by treating the ovarian membrane with a protease. The cosmetic product can be used as any one of (1) a cosmetic product for inhibiting skin allergy symptoms; (2) a cosmetic product for improving skin conditions for the lightening of pigmentation and freckles, the reduction of skin dullness, and the whitening of skin; (3) a cosmetic product for improving darkened pores; and (4) a cosmetic product for increasing the amount of sebum on skin. In the cosmetic products for improving darkened pores and for increasing the amount of sebum on skin, the component obtained by treating The Salmonidae fish ovarian membrane with a protease is preferably grains having an average grain size of 200 to 1400 nm.
Description
Technical field
The present invention relates to a kind of cosmetic material.Specifically, relate to any in the following cosmetic material, promptly suppress the skin allergy symptom with the material of making up; Desalination moth patch on the skin and freckle, elimination dull-looking skin and the colour of skin of whitening etc. are improved skin with the material of making up; Improve pore blackhead cosmetic material; Increase the skin sebum level with the material of making up.
Technical background
Up to now; Following method has been known; The ovary membrane (fish roe crust) that is about to Fish is in advance with after the Ozone Water processing, and enzyme decomposes the proteinic myofibrillar protein as its formation, extracts the method (seeing that the Japanese documentation spy opens the 2004-73186 communique) of aminoacid and peptide.
Above-mentioned aminoacid and peptide can be used as biological active substances or food additive uses, and concrete is exactly, and has ACE and suppresses active, plays a role as the medicament (depressor) that suppresses hypertension.
In addition, following method has been known, promptly uses protease--proteinase is handled the ovary membrane of gadidae and clupeidae Fish, and the composition that obtains is used (seeing that the Japanese documentation spy opens the 2006-225270 communique) as cosmetic formulations.
Above-mentionedly handle the ovary membrane of speckle Gyrinocheilidae, gadidae and clupeidae Fish and the composition that obtains, have the function of gonadal hormone is carried out activation and inhibition, made the effect (sex hormone regulation effect) of the balance normalization of gonadal hormone through proteinase.And above-mentioned cosmetic formulations suppresses sebum secretion through the sex hormone regulation effect of mentioned component, has effect to improving acne, pustule, pachylosis and wrinkle etc.
Below also have a kind of method for known, be about to handle the ovary membrane of salmon and the extract that extracts increases index elevator in age, IGF-1 value elevator, pachylosis improving agent as the acceleration pulse wave and uses (seeing No. 3946239 communique of Japan Patent, No. 3946238 communique, No. 3899116 communique) through protease.
But; For the extract that in the ovary membrane of Fish, extracts, whether it has above-mentionedly brings high blood pressure down, other effects outside the modulability hormone, unknown; And; Even it is restricted to the extract that from the ovary membrane of salmon, extracts, increase index castering action in age, IGF-1 value effect of increasing, improve outside the pachylosis effect at the acceleration pulse wave, do not know whether also have other effects yet.Therefore, for the extract that in the ovary membrane of salmon fishes, extracts, develop widely purposes and expected.
Summary of the invention
In view of said each point, the objective of the invention is to, the ovary membrane self of salmon fishes is provided or the new purposes of the composition that extracts in this ovary membrane certainly.
In order to reach above-mentioned purpose, the invention provides a kind of cosmetic material, it is characterized in that, contain the ovary membrane self of salmon fishes or the composition that extracts through protease this ovary membrane of processing.
Known above-mentioned salmon fishes has 11 types 211 kinds, can enumerate out such as vendace, red salmon, Coho fish, Squaliobarbus ourriculus, oriental cherry Squaliobarbus ourriculus, big Squaliobarbus ourriculus (king salmon) etc.The ovary membrane of above-mentioned salmon fishes is the fish roe crust of above-mentioned salmon fishes, can obtain through only washing from ovary the crust of extracing behind the fish roe.
Above-mentioned protease can be that any ability is divided the proteinic material that is deconstructed into salmon roe nest film.
The cosmetic material that the present invention relates to can be used as the cosmetic material of preparation inhibition skin allergy symptom and uses.Because the cosmetic material that the present invention relates to has the effect of antihistaminic, allergic symptom such as therefore can effectively suppress skin rubefaction that histamine causes, itch.
The cosmetic material that the present invention relates to can be used as the cosmetic material that preparation improves the skin that causes because of melanic generation and uses.Because the cosmetic material that the present invention relates to has the effect that melanin generates that suppresses, therefore has the effect that moth patch on the skin that desalination causes because of melanin generates and freckle, elimination dull-looking skin and the colour of skin of whitening etc. are improved skin.
The cosmetic material that the present invention relates to can also be used as the cosmetic material that preparation improves the blackhead of pore.In human body, for example on the pore at positions such as buccal, forehead, nose blackhead can appear.Above-mentioned pore blackhead is that the mixture that is called as sebum and the cutin etc. of horn plug accumulates in the pore, and the dirt that adheres on the horn plug forms through the peroxidating blackening, or the pigementation blackening that causes because of the melanin in the pore periphery skin forms, and this has been known.The cosmetic material that the present invention relates to has the effect that alleviates above-mentioned pore blackhead, therefore can improve the situation of above-mentioned pore blackhead.
The cosmetic material that the present invention relates to can also be used as the cosmetic material of preparation increase skin sebum level.For example in air dried winter etc., the sebum level of human body can descend, and this has just strengthened the evaporation capacity of moisture of skin, thereby causes xerosis cutis.Follow the drying of skin, can produce various symptoms such as texture chap such as skin, wrinkle increase, sensitivity that outside stimulus is become.Because the cosmetic material that the present invention relates to has the effect that increases the dry skin sebum level, therefore can alleviate, improve above-mentioned xerosis cutis and the various symptoms that produce.
In addition, the cosmetic material that the present invention relates to is characterized in that, handles the ovary membrane of salmon fishes and the composition that extracts is that its mean particle diameter is the particle between 200～1400nm through protease.Because above-mentioned particle has the mean particle diameter in the above-mentioned scope, when using, be easy to skin absorbs as the material of making up, can obtain better effect.
When the mean particle diameter of above-mentioned particle surpasses 1400nm, be not easy to skin absorbs, and during the not enough 200nm of mean particle diameter, tinyization itself is just difficult.
Description of drawings
Fig. 1 is illustrated in whole face and smears the cosmetic material 2 all sketch maps of the tangible pore quantity of blackheads afterwards that are used to improve the pore blackhead that the present invention relates to.
The tangible pore of blackhead reduces the sketch map of quantity in Fig. 2 presentation graphs 1.
Fig. 3 is illustrated in the sketch map that the sebum level after 2 weeks is expected in cosmetic that cheek smears being used to of the present invention relates to and increase the skin sebum level.
The sketch map of the recruitment of sebum level in Fig. 4 presentation graphs 3.
Fig. 5 is illustrated in the sketch map that the sebum level after 2 weeks is expected in cosmetic that forehead smears being used to of the present invention relates to and increase the skin sebum level.
The sketch map of the recruitment of sebum level in Fig. 6 presentation graphs 5.
The specific embodiment
Next, specify embodiment of the present invention with reference to accompanying drawing.
Cosmetic material in this embodiment contains the ovary membrane self of salmon fishes or the composition that extracts through protease this ovary membrane of processing.
Above-mentioned salmon fishes can be enumerated out such as vendace, red salmon, Coho fish, Squaliobarbus ourriculus, oriental cherry Squaliobarbus ourriculus, big Squaliobarbus ourriculus (king salmon) etc.The ovary membrane of above-mentioned salmon fishes is the fish roe crust of above-mentioned salmon fishes, can obtain through only washing from ovary the crust of extracing behind the fish roe.After above-mentioned washing, the cosmetic material in this embodiment can directly use the ovary membrane of the above-mentioned salmon fishes after agitator, grinding pot and mulser etc. are pulverized, and also can use through protease and handle above-mentioned ovary membrane and the composition that extracts.
Use is handled above-mentioned ovary membrane and during the composition (hereinafter to be referred as " ovary membrane extraction composition ") that extracts, ovary membrane extracts composition and can modulate by following method through protease.
At first; Ovary membrane with the salmon fishes that obtains through said method is a raw material; To this ovary membrane, with ovary membrane: stir, mix after the weight ratio of water=1: 1～1: 3 is added water, again protease is added in the scope of 1～3 percentage by weight with respect to the ovary membrane overall weight; Heating is 30 minutes～5 hours under 45～55 ℃ temperature, and the ideal time is 2 hours.Like this, in the composition of above-mentioned ovary membrane,, obtain the aqueous solution of this composition through the stripping in water of the zymolytic composition of above-mentioned albuminolysis at this point.
Then, eliminate the activity of the above-mentioned protease that contains in the above-mentioned aqueous solution.Above-mentioned elimination activity can be through for example carrying out 5 minutes method of above-mentioned aqueous solution heating under 90 ℃ temperature.
Next use the above-mentioned aqueous solution of wire netting simple filtration about 30, remove thick materials such as undecomposed ovary membrane.In the filtrate that obtains, add activated carbon then, this filtrate is carried out deodorize, decolouring, defat.The deodorize of this filtrate, decolouring, defat can be performed as follows, and promptly with respect to the overall weight as the ovary membrane of above-mentioned raw materials, in the scope of 2～4 percentage by weights, in this filtrate, add activated carbon, and heating is 30 minutes under 60 ℃ temperature for example.
After using above-mentioned activated carbon to carry out deodorize, decolouring, ungrease treatment; For example filter above-mentioned filtrate with filter press; The filtrate that obtains is in decompression and is for example concentrating under 60 ℃ the temperature, under 80 ℃ temperature for example, keep then and sterilized in 10 minutes.Then, with the above-mentioned filtrate behind the heat drying method dry sterilization, extract composition thereby can obtain above-mentioned ovary membrane.Above-mentioned ovary membrane extracts in the composition and contains aminoacid, peptide, vitamin, mineral, saccharide, enzyme, nucleic acid and metabolite thereof, various growth factor, cytokine etc.
The mean particle diameter that above-mentioned ovary membrane extracts composition can be refined as in the scope between 200～1400nm.For the ovary membrane that obtains above-mentioned refinement extracts composition; Can the above-mentioned filtrate after the above-mentioned sterilization be handled under the pressure of 980～2940MPa with the supertension mulser; With the heat drying machine the above-mentioned filtrate of handling through the supertension mulser is carried out drying then, just can obtain to have the ovary membrane extraction composition of the mean particle diameter in the above-mentioned scope.
Cosmetic material in this embodiment, owing to contain above-mentioned ovary membrane self or above-mentioned ovary membrane extraction composition, though reason is not very clear and definite, it can be used as following any one cosmetic material at least and uses.
(1) is used to suppress the cosmetic material of skin allergy symptom;
(2) be used to desalinate the cosmetic material that moth patch on the skin and freckle, elimination dull-looking skin and the colour of skin of whitening etc. are improved skin;
(3) be used to improve the cosmetic material of pore blackhead;
(4) be used to increase the cosmetic material of skin sebum level.
Cosmetic material in this embodiment; To the ovary membrane self of above-mentioned salmon fishes or the composition that extracts through protease this ovary membrane of processing; Can add oil, interfacial agent, vitamin, UV absorbent, viscosifier, wetting agent, adjuvant, antiseptic and coloring agent etc. according to demand and modulate according to usual method.The form of expecting of making up is any; Solution shape, paste, bulk, gel, spongy, air bubble-shaped, solid, shaped, graininess, use such as Powdered can be processed, astringent, cream, ointment, shampoo, emulsion, facial film, scented tea, oil, fancy soap, cleansing milk, spice, perfume, bathing agent, shampoo and hair care breast etc. can be used for.
In addition, above-mentioned ovary membrane extracts composition and also can be used as pore blackhead improving agent or skin sebum level dose, or orally uses as health food.When above-mentioned ovary membrane extraction composition uses as health food, for example can add excipient and be made into shapes such as tablet.
Represent embodiments of the invention and reference examples below.
Embodiment 1
Select for use Hokkaido, Japan to produce whitefish, take 100g after the ovary membrane behind the taking-up fish roe in its ovary is cleaned, add pure water 100ml; (Panasonic Electric Equipment Industrial Co.,Ltd makes with agitator; Product type: MX-X107) pulverize 10 times, each 1 minute, obtain ground product.Then, above-mentioned ground product is ground in grinding pot, obtain sample behind the metal mesh filter with 300 meshes.
The sample that obtains in the present embodiment is the ground product of whitefish ovary membrane.Then, carry out following antihistaminic test with the sample that obtains in the present embodiment.
To above-mentioned antihistaminic test, at first said sample is diluted to the solid composition and accounts for 0.2%, the modulation sample solution with pure water.
Then; To (Slc:Wister is a male wister rat from mouse; About 4～9 weeks are big) the fat cell that extracts of intraperitoneal swim among the liquid 1.2ml; (sigma-aldrich corp is produced, trade name: complex 48/80), be modulated into the mixed liquor that sample solution concentration is 1 μ g/ml to add the free agent of said sample solution 0.2ml and histamine.
Then, after cultivating 15 minutes under 37 ℃ the condition, the cooling stopped reaction is done solid-liquid separation with reactant liquor with above-mentioned mixed liquor, from suspension, extracts free histamine and also makes with extra care.Next, make purified histamine produce color with OPA, the measurement excitation wavelength is that 360nm, wavelength of fluorescence are the fluorescence absorbance of 450nm, calculates the free suppression ratio of histamine by following formula.
Histamine suppression ratio (%)={ 1-(A-C)/(B-C) } * 100 of dissociating
In the formula, the fluorescence intensity of free histamine after A representes to make sample and the coexistence of fat cell and adds the free agent of histamine; B representes in fat cell to add the fluorescence intensity of free histamine after the free agent of histamine; C representes from fat cell the fluorescence intensity of free histamine naturally.A, B, C are the result that measured value deducts the blind check value.
The result is as shown in table 1.
Add the 300ml pure water in the sample that in embodiment 1, obtains, (mizuho Industrial Co., Ltd makes, trade name: ultra micron stirring powder crushing device M110-E/H) under 150MPa pressure, pulverize 2 times, obtain sample with the high-pressure emulsification machine.
The sample of obtaining in the present embodiment is the tiny ground product of the ovary membrane of whitefish.Below the sample in using present embodiment, obtained, do the free inhibition test of identical histamine with embodiment 1, calculate the free suppression ratio of histamine.
The result is as shown in table 1.
Embodiment 3
After the pH value of the sample of obtaining among the embodiment 2 (aqueous solution) adjusted to 7.2～7.5; (English name: Actinase E) (Kaken Pharmaceufical Co., Ltd.'s manufacturings) as protease, followingly stirs 3 hours limits and it is produced react at 40 ℃ to add 0.5g protease XIV.
Then, the protease that contains in the above-mentioned aqueous solution was heated 5 minutes under 90 ℃ temperature, make above-mentioned protease loss of activity after, with the filter simple filtration of 30 meshes, remove thick materials such as undecomposed ovary membrane.
Then, the 500g activated carbon is added in the filtrate after the above-mentioned simple filtration, heating is 30 minutes under 60 ℃ temperature.After this filtrate carried out defat, decolouring, deodorize, filter above-mentioned filtrate with filter press, with the filtrate that obtains under reduced pressure, concentrate, sterilization, the above-mentioned filtrate after will sterilize then is dry through the heat drying machine, the acquisition sample.
The sample that obtains in the present embodiment is that the ovary membrane of whitefish extracts composition.Below the sample in using present embodiment, obtained, do the free inhibition test of identical histamine with embodiment 1, calculate the free suppression ratio of histamine.
The result is as shown in table 1.
Table 1
| The histamine suppression ratio (%) that dissociates | |
| Embodiment 1 | 77.9 |
| |
69.2 |
| Embodiment 3 | 69.9 |
Can obviously find out from table 1, handle the ovary membrane of salmon fishes and the ovary membrane that extracts extracts composition through protease among the ovary membrane of the salmon fishes among embodiment 1 and the embodiment 2 and the embodiment 3, all have the free effect of good restraining histamine.Therefore, apparently, extract composition, can obtain to have the cosmetic material of antihistaminic effect through the ovary membrane of use salmon fishes or the ovary membrane of salmon fishes.Use the material of to make up, the effect of allergic symptom such as can expect to suppress skin rubefaction that histamine causes, itch.
Embodiment 4
Select for use Hokkaido, Japan to produce whitefish, take 100g after the ovary membrane behind the taking-up fish roe in its ovary is cleaned, add pure water 100ml; (Panasonic Electric Equipment Industrial Co.,Ltd makes with agitator; Product type: MX-X107) pulverize 10 times, each 1 minute, obtain ground product.Then, above-mentioned ground product is ground in grinding pot, obtain sample behind the metal mesh filter with 300 meshes.
The sample that obtains in the present embodiment is the ground product of whitefish ovary membrane.Then, suppress melanin with the sample that obtains in the present embodiment and generate test.
Generate in the test at above-mentioned inhibition melanin, at first with MEM culture medium (hereinafter to be referred as " the MEM culture medium that contains 10%FBS ") the dilution said sample that contains 10%FBS.As the A of testing liquid, the said sample that is diluted to 300ppm is as the B of testing liquid with the said sample that is diluted to 100ppm.
Then with the MEM culture medium culturing melanin cellulation that contains 10%FBS, i.e. B-16 mouse melanin tumor cell.Use above-mentioned culture medium, will adjust to 1 * 10
5The B-16 mouse melanin tumor cell of cell/ml is sowed 5ml respectively in the flask of 10 25ml.With the B-16 mouse melanin tumor cell of in above-mentioned each flask, having sowed, be adjusted to 5% couveuse with gas concentration lwevel and cultivated 24 hours down at 37 ℃.
Then; After confirming that the B-16 mouse melanin tumor cell adheres to above-mentioned each flask fully, from above-mentioned each flask, remove the MEM culture medium that contains 10%FBS, the substitute is; In 3 above-mentioned flasks, add the 5ml A of testing liquid; In 3 above-mentioned flasks, add the 5ml B of testing liquid, in 3 above-mentioned flasks, add the MEM culture medium that contain 10%FBS of 5ml, and cultivated 6 days as standard of comparison.At the 3rd day that cultivates, remove the A of testing liquid, the B of testing liquid in the above-mentioned flask, contain the MEM culture medium of 10%FBS, add identical liquid 5ml more respectively.
After cultivating end, after trypsin treatment, reclaim the B-16 mouse melanin tumor cell.
The whiteness of the B-16 mouse melanin tumor cell that reclaims detects by an unaided eye.Observed result is as shown in table 2.In table 2, the whiteness of " ± " expression and standard of comparison equal extent; "+", " ++ ", " +++" expression "+" is many more, and the whitening degree is high more.
Embodiment 5
In the sample that in embodiment 4, obtains, add the 300ml pure water; (mizuho Industrial Co., Ltd makes with the high-pressure emulsification machine; Name of product: ultra micron stirring powder crushing device M110-E/H), under the processing pressure of 150MPa, carry out 2 times and pulverize operation, obtain sample.
The sample that obtains in the present embodiment is the tiny ground product of whitefish ovary membrane.Except that the sample that uses present embodiment to obtain, make identical melanin with embodiment 4 and generate inhibition test.Observed result is as shown in table 2.
Embodiment 6
After the pH value of the sample (aqueous solution) that obtains among the embodiment 5 adjusted to 7.2～7.5; Add 0.5g protease XIV (English name: Actinase E) (Kaken Pharmaceufical Co., Ltd.'s manufacturings) on 3 hours limits of 40 ℃ of following stirrings it is reacted as protease.
The above-mentioned above-mentioned aqueous solution that contains protease was heated 5 minutes under 90 ℃ temperature, above-mentioned protease is lost activity after, with the wire netting simple filtration of 30 meshes, remove thick materials such as undecomposed ovary membrane.
Add the 500g activated carbon in the filtrate that after above-mentioned simple filtration, obtains, heating is 30 minutes under 60 ℃ temperature.After this filtrate carried out defat, decolouring, deodorize, filter above-mentioned filtrate with filter press, with the filtrate that obtains under reduced pressure, concentrate, sterilization.Then, with the above-mentioned filtrate behind the heat drying machine dry sterilization, obtain sample.
The sample that obtains in the present embodiment is that the ovary membrane of whitefish extracts composition.The sample that in using present embodiment, obtains, make identical melanin with embodiment 4 and generate inhibition test.Observed result is as shown in table 2.
Table 2
| Whiteness | ||
| Embodiment 4 | The A of testing liquid | ++ |
| The B of testing liquid | +++ | |
| Embodiment 5 | The A of testing liquid | +++ |
| The B of testing liquid | +++ | |
| Embodiment 6 | The A of testing liquid | ++ |
| The B of testing liquid | +++ |
Can clearly find out from table 2, handle the ovary membrane of salmon fishes and the ovary membrane that extracts extracts composition through protease among the ovary membrane of the salmon fishes among embodiment 4 and the embodiment 5 and the embodiment 6, all have the effect that good restraining melanin generates.Therefore, apparently, extract composition, can obtain to have the cosmetic material that suppresses the melanin nucleus formation through the ovary membrane of use salmon fishes or the ovary membrane of salmon fishes.Use this cosmetic material can expect to desalinate the effect that moth patch on the skin and freckle, elimination dull-looking skin and the colour of skin of whitening etc. are improved skin.
Embodiment 7
Select for use Hokkaido, Japan to produce whitefish, take 100g after the ovary membrane behind the taking-up fish roe in its ovary is cleaned, add pure water 100ml; (Panasonic Electric Equipment Industrial Co.,Ltd makes with agitator; Product type: MX-X107) pulverize 10 times, each 1 minute, obtain ground product.Then, above-mentioned ground product is further ground in grinding pot, with the metal mesh filter of 300 meshes.
Then; Add the 300ml pure water in the object after filtering; (mizuho Industrial Co., Ltd makes with the supertension mulser; Name of product: ultra micron stirring powder crushing device M110-E/H) under the processing pressure of 150MPa, pulverize, operate 2 times, obtain the aqueous solution of the tiny ground product of whitefish ovary membrane.
After above-mentioned pH value of aqueous solution adjusted to 7.2～7.5, add 0.5g protease XIV (English name: Actinase E) (Kaken Pharmaceufical Co., Ltd.'s manufacturings) make its reaction as protease on 3 hours limits of 40 ℃ of following stirrings.
Then, under 90 ℃ temperature the heating 5 minutes, make the above-mentioned protease loss of activity that contains in the above-mentioned aqueous solution after, with the wire netting simple filtration of 30 meshes, remove thick materials such as undecomposed ovary membrane.
In the filtrate that obtains through simple filtration, add the 500g activated carbon; Heating is 30 minutes under 60 ℃ temperature; After this filtrate carried out defat, decolouring, deodorize, filter above-mentioned filtrate with filter press, with the filtrate that obtains under reduced pressure, concentrate, sterilization; Again with the filtrate that obtains under the reduced pressure and for example concentrating under 60 ℃ the temperature, obtain the filtrate that the whitefish ovary membrane extracts composition.
Then, with above-mentioned supertension mulser above-mentioned spissated filtrate is handled under the pressure of 980～2940MPa, the ovary membrane of above-mentioned whitefish being extracted into being divided into mean particle diameter is the micropartical between 200～1400nm.Then, with the filtrate that the dry supertension mulser of heat drying machine is handled, the salmonid ovary membrane that obtains above-mentioned corpusculed extracts composition (Sample A).
Use said sample A,,, obtain to be used to improve the astringent of pore blackhead according to usual method according to the allotment ratio of table 3.Equally, use said sample A,,, obtain to be used to improve the emulsion of pore blackhead according to usual method according to the allotment ratio of table 4.
Table 3
| Composition | Weight (g) |
| Sample A | ?0.5 |
| Pure water | ?89.781 |
| 1,3 butylene glycol | ?3.0 |
| Ethanol | ?4.0 |
| Glycerol | ?2.0 |
| Nipagin | ?0.1 |
| Polyethylene glycol oxide solidifies Oleum Ricini | ?0.1 |
| Natural Vitamin E | ?0.019 |
| Hyaluronate sodium | ?0.5 |
| Add up to | ?100.0 |
Table 4
| Composition | Weight (g) |
| Sample A | ?0.5 |
| Pure water | ?92.375 |
| 1,3 butylene glycol | ?2.0 |
| Glycerol | ?3.0 |
| 1, the 2-pentanediol | ?2.0 |
| Polyethylene glycol oxide solidifies Oleum Ricini | ?0.1 |
| Xanthan gum | ?0.025 |
| Add up to | ?100.0 |
Please 12 38～42 years old healthy womens people on probation, used for 2 weeks every day 2 times sooner or later, the back is smeared above-mentioned pore blackhead on the face and is improved with astringent and the improvement of above-mentioned pore blackhead and use emulsion whole in washing one's face.Everybody people on probation should be WUHUAFEN allergy and salmon anaphylaxis, non-smoking, does not take the common drug that comprises oral contraceptive, the personage who did not accept beautifying medical, non-pregnancy and non-lactation period.
Smear begin and smeared for 2 weeks after, (Inforward of Co., Ltd. makes, trade name: the muscle image analysis apparatus) in lighting condition shooting down uniformly, obtain the black white image of whole facial skin with the muscle image analysis apparatus.According to obtaining the tangible pore quantity of skin image measuring and calculating blackhead.Being defined as of pore here, in the image of skin, when the tone light and shade is presented at 0～255 color range (darkness near 0, the lightness near 255), color range be 0～215 and area at 0.1～0.6mm
2The zone.Meet in the pore definitional part, color range be 0～40 region memory pore be defined as " the tangible pore of blackhead ".
Fig. 1 representes the meansigma methods of the tangible pore number of above-mentioned 12 philtrum blackheads; The minimizing quantity of the tangible pore of blackhead in Fig. 2 presentation graphs 1.In addition, be " 0 week " with beginning to smear preface among Fig. 1, smear lasting 2 all postscripts and be " 2 week ".
Reference examples 1
In this reference examples, the pore blackhead that at first replaces in the foregoing description 7 improves astringent, according to the allotment ratio of table 5, uses usual method, obtains not contain soothing agent (playing the medicine of the psychological placebo effect) astringent of said sample A.Above-mentioned soothing agent astringent uses the pure water with weight except that replacing said sample A, and other aspects are identical with astringent with the pore blackhead improvement in the foregoing description 7.
Emulsion is used in the pore blackhead improvement that replaces in the foregoing description 7, according to the allotment ratio of table 6, uses usual method, obtains the soothing agent emulsion that does not contain said sample A.Above-mentioned soothing agent emulsion is used the pure water of identical weight except that replacing said sample A, and it is identical that other aspects and pore blackhead among the embodiment 7 improve emulsion.
Table 5
| Composition | Weight (g) |
| Pure water | ?90.281 |
| 1,3 butylene glycol | ?3.0 |
| Ethanol | ?4.0 |
| Glycerol | ?2.0 |
| Nipagin | ?0.1 |
| Polyethylene glycol oxide solidifies Oleum Ricini | ?0.1 |
| Natural Vitamin E | ?0.019 |
| Hyaluronate sodium | ?0.5 |
| Add up to | ?100.0 |
Table 6
| Composition | Weight (g) |
| Pure water | ?92.875 |
| 1,3 butylene glycol | ?2.0 |
| Glycerol | ?3.0 |
| 1, the 2-pentanediol | ?2.0 |
| Nipagin | ?0.1 |
| Xanthan gum | ?0.025 |
| Add up to | ?100.0 |
Invite with embodiment 7 12 different healthy womens of 38～42 years old and try out the people, used for 2 weeks every day 2 times sooner or later, back in washing one's face in whole above-mentioned astringent and the above-mentioned emulsion of smearing on the face.Everybody people on probation should be WUHUAFEN allergy and salmon anaphylaxis, non-smoking, does not take the common drug that comprises oral contraceptive, the personage who does not accept beautifying medical, non-pregnancy and non-lactation period.
Then, identical with the foregoing description, the tangible pore number of measuring and calculating blackhead.Fig. 1 representes the meansigma methods of the tangible pore quantity of above-mentioned 12 people's on probation blackhead; The minimizing quantity of the tangible pore of blackhead in Fig. 2 presentation graphs 1.
As shown in Figure 1, improve with emulsion (embodiment 7) through using pore blackhead in this embodiment to improve with the pore blackhead in astringent and this embodiment, compare smear beginning before, the tangible pore quantity of blackhead obviously continues the back minimizing of 2 weeks smearing.In contrast to this, use soothing agent astringent and soothing agent emulsion (reference examples 1), compare smear beginning before, the tangible pore quantity of blackhead obviously increases after continuing for 2 weeks smearing slightly.
As shown in Figure 2, use emulsion through using the pore blackhead improvement in this embodiment with the improvement of the pore blackhead in astringent and this embodiment, the tangible pore quantity of blackhead obviously significantly reduces.
Therefore, obviously, the pore blackhead improvement of on skin, smearing in this embodiment is used emulsion with the improvement of the pore blackhead in astringent and this embodiment, can alleviate, improve the pore blackhead.
Embodiment 8
Select for use Hokkaido, Japan to produce whitefish, take 100g after the ovary membrane behind the taking-up fish roe in its ovary is cleaned, add pure water 100ml; (Panasonic Electric Equipment Industrial Co.,Ltd makes with agitator; Product type: MX-X107) pulverize 10 times, each 1 minute, obtain ground product.Then, above-mentioned ground product is further ground in grinding pot, filter with the wire netting of 300 meshes.
In filtrate, add the 300ml pure water; (mizuho Industrial Co., Ltd makes with the supertension mulser; Name of product: ultra micron stirring powder crushing device M110-E/H) under the processing pressure of 150MPa, pulverize, operate 2 times, obtain the aqueous solution of the tiny ground product of whitefish ovary membrane.
After above-mentioned pH value of aqueous solution adjusted to 7.2～7.5, add 0.5g protease XIV (English name: Actinase E) (Kaken Pharmaceufical Co., Ltd.'s manufacturings) make its reaction as protease on 3 hours limits of 40 ℃ of following stirrings.
Then, under 90 ℃ temperature the heating 5 minutes, make the protease loss of activity that contains in the above-mentioned aqueous solution after, with the wire netting simple filtration of 30 meshes, remove thick materials such as undecomposed ovary membrane.
Add the 500g activated carbon in the filtrate that after above-mentioned simple filtration, obtains, heating is 30 minutes under 60 ℃ temperature.After this filtrate carried out defat, decolouring, deodorize; Filter above-mentioned filtrate with filter press; The filtrate that obtains is concentrated under reduced pressure, sterilizes; Again with the filtrate that obtains in reduced pressure and in for example concentrating under 60 ℃ the temperature, the ovary membrane that obtains whitefish extracts the filtrate of composition.
Then, with above-mentioned supertension mulser above-mentioned spissated filtrate is handled under the pressure between 980～2940MPa, the ovary membrane of above-mentioned whitefish being extracted into being divided into mean particle diameter is the micropartical between 200～1400nm.Then, with the filtrate that the dry above-mentioned supertension mulser of heat drying machine is handled, the salmonid ovary membrane that obtains above-mentioned corpusculed extracts composition (Sample A).
Use said sample A,,, obtain to be used to increase the astringent of skin sebum level according to usual method according to the allotment ratio of table 7.Equally, use said sample A,,, obtain to be used to increase the emulsion of skin sebum level according to usual method according to the allotment ratio of table 8.
Table 7
| Composition | Weight (g) |
| Sample A | ?0.5 |
| Pure water | ?89.781 |
| 1,3 butylene glycol | ?3.0 |
| Ethanol | ?4.0 |
| Glycerol | ?2.0 |
| Nipagin | ?0.1 |
| Polyethylene glycol oxide solidifies Oleum Ricini | ?0.1 |
| Natural Vitamin E | ?0.019 |
| Hyaluronate sodium | ?0.5 |
| Add up to | ?100.0 |
Table 8
| Composition | Weight (g) |
| Sample A | ?0.5 |
| Pure water | ?92.375 |
| 1,3 butylene glycol | ?2.0 |
| Glycerol | ?3.0 |
| 1, the 2-pentanediol | ?2.0 |
| Polyethylene glycol oxide solidifies Oleum Ricini | ?0.1 |
| Xanthan gum | ?0.025 |
| Add up to | ?100.0 |
Invite 12 healthy womens of 38～42 years old to try out the people, used for 2 weeks every day 2 times sooner or later, astringent and the above-mentioned emulsion that is used to increase the skin sebum level that is used to increase the skin sebum level smeared in the back above cheek and eyebrow in washing one's face.Everybody people on probation should be WUHUAFEN allergy and salmon anaphylaxis, non-smoking, does not take the common drug that comprises oral contraceptive, the personage who did not accept beautifying medical, non-pregnancy and non-lactation period.
After smearing beginning and smearing for 2 weeks, use sebum level meter (Courage+Khazaka manufactured, name of product: Sebumeter), measure the sebum level of cheek and eyebrow top.Concrete operations do, at first the top with tool housing touches measured position (cheek or brows top), and the thickness that the sebum that makes surface, measured position exposes attached to this top ends certainly is on the opaque plastics band of 0.1mm.Then, insert body, measure the transparency change of this tape, thereby draw the sebum level on surface, measured position through the light accepting part of body in the top ends of shell.The unit of this sebum level is with μ g/cm
2Expression.
Fig. 3 representes the meansigma methods of above-mentioned 12 people's on probation cheek sebum level.The recruitment part of the sebum level in Fig. 4 presentation graphs 3.Fig. 5 representes the meansigma methods of the sebum level that above-mentioned 12 people's on probation brows are above.What Fig. 6 write down is the recruitment part of sebum level among Fig. 5.In Fig. 3 and Fig. 5, smear before the beginning with " 0 week " expression, continue to smear 2 Zhou Houyong " 2 week " expression.
Reference examples 2
In this reference examples, astringent is used in the skin sebum level increase that at first replaces in the foregoing description 8, according to the allotment ratio of table 9, according to usual method, obtains not contain soothing agent (playing the medicine of the psychological placebo effect) astringent of said sample A.Above-mentioned soothing agent astringent, except that the pure water with same weight replaces the said sample A, other aspects are identical with astringent with the skin sebum level increase among the embodiment 8.
In addition, emulsion is used in the skin sebum level increase that replaces in the foregoing description 8, according to the allotment ratio of table 10, according to usual method, obtains not contain the soothing agent emulsion of said sample A.This soothing agent emulsion replaces the said sample A except that the pure water with same weight, and other aspects are identical with emulsion with the skin sebum level increase among the embodiment 8.
Table 9
| Composition | Weight (g) |
| Pure water | ?90.281 |
| 1,3 butylene glycol | ?3.0 |
| Ethanol | ?4.0 |
| Glycerol | ?2.0 |
| Nipagin | ?0.1 |
| Polyethylene glycol oxide solidifies Oleum Ricini | ?0.1 |
| Natural Vitamin E | ?0.019 |
| Hyaluronate sodium | ?0.5 |
| Add up to | ?100.0 |
Table 10
| Composition | Weight (g) |
| Pure water | ?92.875 |
| 1,3 butylene glycol | ?2.0 |
| Glycerol | ?3.0 |
| 1, the 2-pentanediol | ?2.0 |
| Nipagin | ?0.1 |
| Xanthan gum | ?0.025 |
| Add up to | ?100.0 |
Then, invite with the foregoing description 8 12 different healthy womens of 38～42 years old and try out the people, used for 2 weeks every day 2 times sooner or later, above-mentioned astringent and above-mentioned emulsion are smeared in the back above cheek and eyebrow in washing one's face.Everybody people on probation should be WUHUAFEN allergy and salmon anaphylaxis, non-smoking, does not take the common drug that comprises oral contraceptive, the personage who did not accept beautifying medical, non-pregnancy and non-lactation period.
Identical with the foregoing description 8, the sebum level of measurement cheek and eyebrow top.Fig. 3 representes the meansigma methods of above-mentioned 12 people's on probation cheek sebum level.The recruitment part of the sebum level in Fig. 4 presentation graphs 3.Fig. 5 representes the meansigma methods of sebum level of above-mentioned 12 people's on probation eyebrow top.What Fig. 6 write down is the recruitment part of sebum level among Fig. 5.
As shown in Figure 3; Through using the skin sebum level in this embodiment to increase with the increase of the skin sebum level in astringent and this embodiment with emulsion (embodiment 8); Compare smear beginning before, the sebum level of cheek obviously increases after continuing to smear for 2 weeks to some extent.In contrast to this, use soothing agent astringent and soothing agent emulsion (contrast falls 2), compare smear before, the sebum level of cheek obviously descends after continuing to smear for 2 weeks to some extent.
As shown in Figure 4, increase with astringent and emulsion through using the skin sebum level in this embodiment, the sebum level of cheek has obvious rising.
As shown in Figure 5, increasing skin sebum level with astringent and this embodiment through the skin sebum level that uses this embodiment increases and use emulsion, compare smear before, the obviously increase to some extent after continuing to smear for 2 weeks of the sebum level of eyebrow top.In contrast to this, use soothing agent astringent and emulsion, compare smear beginning before, the sebum level of eyebrow top obviously descends after continuing to smear for 2 weeks to some extent.
As shown in Figure 6, use emulsion through using the skin sebum level increase in this embodiment with the skin sebum level increase of astringent and this embodiment, the sebum level of eyebrow top obviously has very big increase.
Therefore; Skin sebum level increase through on skin, smearing in this embodiment is used the astringent emulsion with the increase of the skin sebum level in astringent and this embodiment; Sebum level is increased, increase and be obvious various symptoms such as outside stimulus sensitivities so can alleviate, improve because of pachylosis, wrinkle that xerosis cutis produces.
Claims (8)
1. the ovary membrane of a salmon fishes is characterized in that as the purposes of the material of making up the ovary membrane of said salmon fishes has the effect of antihistaminic, suppresses the skin allergy symptom as preparation and uses with the material of making up.
2. the ovary membrane of a salmon fishes is as the purposes of the material of making up; It is characterized in that; The ovary membrane of said salmon fishes has the effect that melanin generates that suppresses, and uses with the material of making up as preparation desalination moth patch on the skin and freckle, elimination dull-looking skin, the U.S. fair-complexioned skin that improves.
One kind through protease handle the ovary membrane of salmon fishes and the composition that extracts as the purposes of the material of making up; It is characterized in that; Saidly handle the ovary membrane of salmon fishes and the composition that extracts has the effect of antihistaminic, suppress the skin allergy symptom as preparation and use with the material of making up through protease.
One kind through protease handle the ovary membrane of salmon fishes and the composition that extracts as the purposes of the material of making up; It is characterized in that; Saidly handle the ovary membrane of salmon fishes and the composition that extracts has the effect that melanin generates that suppresses, use with the material of making up as preparation desalination moth patch on the skin and freckle, elimination dull-looking skin, the U.S. fair-complexioned skin that improves through protease.
One kind through protease handle the ovary membrane of salmon fishes and the composition that extracts as the purposes of the material of making up; It is characterized in that; Saidly handle the ovary membrane of salmon fishes and the composition that extracts has the effect that suppresses the pore blackhead, improve the pore blackhead material of making up as preparation and use through protease.
6. as claimed in claim 5 through protease handle the ovary membrane of salmon fishes and the composition that extracts as the purposes of the material of making up; It is characterized in that; Handle the ovary membrane of above-mentioned salmon fishes and the composition that extracts is the particle of mean particle diameter between 200～1400nm through protease.
One kind through protease handle the ovary membrane of salmon fishes and the composition that extracts as the purposes of the material of making up; It is characterized in that; Saidly handle the ovary membrane of salmon fishes and the composition that extracts has the effect of the sebum level that increases dry skin, increase the skin sebum level as preparation and use with the material of making up through protease.
8. as claimed in claim 7 through protease handle the ovary membrane of salmon fishes and the composition that extracts as the purposes of the material of making up; It is characterized in that; Handle the ovary membrane of above-mentioned salmon fishes and the composition that extracts is the particle of mean particle diameter between 200～1400nm through protease.
Applications Claiming Priority (12)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006346552A JP3981397B1 (en) | 2006-12-22 | 2006-12-22 | Cosmetics |
| JP2006346553A JP4005113B1 (en) | 2006-12-22 | 2006-12-22 | Cosmetics |
| JP2006-346553 | 2006-12-22 | ||
| JP2006-346552 | 2006-12-22 | ||
| JP2006346552 | 2006-12-22 | ||
| JP2006346553 | 2006-12-22 | ||
| JP2007231605 | 2007-09-06 | ||
| JP2007231606A JP4792588B2 (en) | 2007-09-06 | 2007-09-06 | Pore darkening agent |
| JP2007-231606 | 2007-09-06 | ||
| JP2007231606 | 2007-09-06 | ||
| JP2007-231605 | 2007-09-06 | ||
| JP2007231605 | 2007-09-06 |
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| CN101204363A CN101204363A (en) | 2008-06-25 |
| CN101204363B true CN101204363B (en) | 2012-07-04 |
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| CN2007103020647A Active CN101204363B (en) | 2006-12-22 | 2007-12-21 | Cosmetic product |
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| JP (1) | JP4005113B1 (en) |
| CN (1) | CN101204363B (en) |
| ES (1) | ES2369730T3 (en) |
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| JP7116950B2 (en) * | 2018-03-29 | 2022-08-12 | 株式会社ヴェントゥーノ | Melanin production inhibitor by marine placenta and collagen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002136274A (en) * | 2000-11-02 | 2002-05-14 | Hitomi Hayabuchi | Crispy food and method for producing the same |
| JP2006225270A (en) * | 2005-02-15 | 2006-08-31 | Yoshihiro Futamura | Tripeptide having sex hormone modification action, extract containing the same, eicosapentaenoic acid-bonded tripeptide, food preparation, cosmetic preparation and endometriosis treating agent containing the same |
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2006
- 2006-12-22 JP JP2006346553A patent/JP4005113B1/en active Active
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- 2007-12-20 ES ES07024856T patent/ES2369730T3/en active Active
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002136274A (en) * | 2000-11-02 | 2002-05-14 | Hitomi Hayabuchi | Crispy food and method for producing the same |
| JP2006225270A (en) * | 2005-02-15 | 2006-08-31 | Yoshihiro Futamura | Tripeptide having sex hormone modification action, extract containing the same, eicosapentaenoic acid-bonded tripeptide, food preparation, cosmetic preparation and endometriosis treating agent containing the same |
Non-Patent Citations (3)
| Title |
|---|
| JP昭57074062A 1982.05.10 |
| JP特开2002136274A 2002.05.14 |
| JP特开2006225270A 2006.08.31 |
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| JP2008156273A (en) | 2008-07-10 |
| JP4005113B1 (en) | 2007-11-07 |
| CN101204363A (en) | 2008-06-25 |
| ES2369730T3 (en) | 2011-12-05 |
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