JP2008086326A - ゲノム性要素を使用する遺伝子の操作および発現 - Google Patents

ゲノム性要素を使用する遺伝子の操作および発現 Download PDF

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JP2008086326A
JP2008086326A JP2007317755A JP2007317755A JP2008086326A JP 2008086326 A JP2008086326 A JP 2008086326A JP 2007317755 A JP2007317755 A JP 2007317755A JP 2007317755 A JP2007317755 A JP 2007317755A JP 2008086326 A JP2008086326 A JP 2008086326A
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expression
target gene
protein
proliferatable
gene manipulation
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Stephen Sherwin
シャーウィン スティーブン
Sue Klapholz
クラフォルツ スー
Arthur Skoultchi
スコールチ アーサー
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Cell Genesys Inc
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Cell Genesys Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/206—Pseudochromosomes, minichrosomosomes of yeast origin, e.g. YAC, 2u
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

【課題】当該分野における従来の課題を解決するための方法の提供。
【解決手段】標的遺伝子座における標的遺伝子の野生型のまたは修飾された哺乳動物タンパク質を製造する方法であって、前記方法は、増幅可能な領域の多数のコピーを含んでなる二次哺乳動物発現宿主細胞を増殖させ、ここで前記増殖可能な領域は前記二次発現宿主に対して異種の標的遺伝子を含んでなり、そして問題のタンパク質および増幅可能な遺伝子を発現させるものであり、これにより前記標的遺伝子が発現されそして前記タンパク質は生産されることなどを含んでなり、その他本願明細書に記載されるような特徴を有する方法。
【選択図】なし

Description

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Claims (1)

  1. 本明細書に記載されるような、ゲノム性要素を使用する遺伝子の操作および発現。
JP2007317755A 1991-05-06 2007-12-07 ゲノム性要素を使用する遺伝子の操作および発現 Pending JP2008086326A (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US69621691A 1991-05-06 1991-05-06

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP4511157A Division JPH06507550A (ja) 1991-05-06 1992-05-05 ゲノム性要素を使用する遺伝子の操作および発現

Publications (1)

Publication Number Publication Date
JP2008086326A true JP2008086326A (ja) 2008-04-17

Family

ID=24796175

Family Applications (2)

Application Number Title Priority Date Filing Date
JP4511157A Withdrawn JPH06507550A (ja) 1991-05-06 1992-05-05 ゲノム性要素を使用する遺伝子の操作および発現
JP2007317755A Pending JP2008086326A (ja) 1991-05-06 2007-12-07 ゲノム性要素を使用する遺伝子の操作および発現

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP4511157A Withdrawn JPH06507550A (ja) 1991-05-06 1992-05-05 ゲノム性要素を使用する遺伝子の操作および発現

Country Status (10)

Country Link
EP (1) EP0584214B1 (ja)
JP (2) JPH06507550A (ja)
KR (1) KR100259933B1 (ja)
AT (1) ATE272121T1 (ja)
AU (1) AU671801B2 (ja)
CA (2) CA2102594C (ja)
DE (1) DE69233387T2 (ja)
NO (1) NO934011L (ja)
SG (1) SG93780A1 (ja)
WO (1) WO1992019255A1 (ja)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048729A (en) 1987-05-01 2000-04-11 Transkaryotic Therapies, Inc. In vivo protein production and delivery system for gene therapy
US6692737B1 (en) 1991-11-05 2004-02-17 Transkaryotic Therapies, Inc. In vivo protein production and delivery system for gene therapy
US5968502A (en) * 1991-11-05 1999-10-19 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6054288A (en) * 1991-11-05 2000-04-25 Transkaryotic Therapies, Inc. In vivo protein production and delivery system for gene therapy
NZ245015A (en) * 1991-11-05 1995-12-21 Transkaryotic Therapies Inc Delivery of human growth hormone through the administration of transfected cell lines encoding human growth hormone, which are physically protected from host immune response; the transfected cells and their production
US6063630A (en) 1991-11-05 2000-05-16 Transkaryotic Therapies, Inc. Targeted introduction of DNA into primary or secondary cells and their use for gene therapy
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6670178B1 (en) 1992-07-10 2003-12-30 Transkaryotic Therapies, Inc. In Vivo production and delivery of insulinotropin for gene therapy
US6531124B1 (en) 1992-07-10 2003-03-11 Transkaryotic Therapies, Inc. In vivo production and delivery of insulinotropin for gene therapy
EP0742285A1 (en) * 1995-05-10 1996-11-13 Rijksuniversiteit Leiden Method of isolating exonic gene segments of eukaryotic genes, exon trapping vectors for use therein
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US7316923B1 (en) 1997-09-26 2008-01-08 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US4889806A (en) * 1987-04-15 1989-12-26 Washington University Large DNA cloning system based on yeast artificial chromosomes
ES2090297T5 (es) * 1989-11-06 2005-03-01 Cell Genesys, Inc. Produccion de proteinas que utilizan recombinacion homologa.
KR0176693B1 (ko) * 1989-12-22 1999-04-01 레지널드 디. 쇼트보그; 제롬 반 쥘렌 조절요소를 사용한 내인성 유전자의 발현을 변형시키는 방법

Also Published As

Publication number Publication date
SG93780A1 (en) 2003-01-21
JPH06507550A (ja) 1994-09-01
EP0584214B1 (en) 2004-07-28
DE69233387T2 (de) 2005-07-21
AU1973292A (en) 1992-12-21
CA2102594A1 (en) 1992-11-07
CA2102594C (en) 2007-07-17
ATE272121T1 (de) 2004-08-15
KR100259933B1 (ko) 2000-06-15
EP0584214A1 (en) 1994-03-02
AU671801B2 (en) 1996-09-12
EP0584214A4 (en) 1995-04-19
DE69233387D1 (de) 2004-09-02
CA2102628A1 (en) 1992-11-07
NO934011D0 (no) 1993-11-05
NO934011L (no) 1994-01-05
WO1992019255A1 (en) 1992-11-12

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