JP2007507238A5 - - Google Patents
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- Publication number
- JP2007507238A5 JP2007507238A5 JP2006534241A JP2006534241A JP2007507238A5 JP 2007507238 A5 JP2007507238 A5 JP 2007507238A5 JP 2006534241 A JP2006534241 A JP 2006534241A JP 2006534241 A JP2006534241 A JP 2006534241A JP 2007507238 A5 JP2007507238 A5 JP 2007507238A5
- Authority
- JP
- Japan
- Prior art keywords
- compound
- solid
- nucleic acid
- cyanine dye
- immobilized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000001875 compounds Chemical class 0.000 claims 33
- 239000007787 solid Substances 0.000 claims 32
- 108020004707 nucleic acids Proteins 0.000 claims 25
- 102000039446 nucleic acids Human genes 0.000 claims 25
- 150000007523 nucleic acids Chemical class 0.000 claims 25
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims 25
- 238000000034 method Methods 0.000 claims 21
- 239000012192 staining solution Substances 0.000 claims 17
- 108020004414 DNA Proteins 0.000 claims 12
- 239000011159 matrix material Substances 0.000 claims 11
- 239000000499 gel Substances 0.000 claims 9
- 229920000642 polymer Polymers 0.000 claims 8
- 102000053602 DNA Human genes 0.000 claims 6
- 231100000804 nongenotoxic Toxicity 0.000 claims 6
- 229920002401 polyacrylamide Polymers 0.000 claims 6
- 239000000243 solution Substances 0.000 claims 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 5
- 229920000936 Agarose Polymers 0.000 claims 4
- 239000011543 agarose gel Substances 0.000 claims 4
- 210000003527 eukaryotic cell Anatomy 0.000 claims 4
- 238000002372 labelling Methods 0.000 claims 4
- 239000000203 mixture Substances 0.000 claims 4
- ACOJCCLIDPZYJC-UHFFFAOYSA-M thiazole orange Chemical compound CC1=CC=C(S([O-])(=O)=O)C=C1.C1=CC=C2C(C=C3N(C4=CC=CC=C4S3)C)=CC=[N+](C)C2=C1 ACOJCCLIDPZYJC-UHFFFAOYSA-M 0.000 claims 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims 3
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 3
- RDOXTESZEPMUJZ-UHFFFAOYSA-N anisole Chemical group COC1=CC=CC=C1 RDOXTESZEPMUJZ-UHFFFAOYSA-N 0.000 claims 3
- 239000007864 aqueous solution Substances 0.000 claims 3
- -1 array Substances 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 3
- 239000011521 glass Substances 0.000 claims 3
- 239000012528 membrane Substances 0.000 claims 3
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 claims 3
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 claims 3
- 239000008351 acetate buffer Substances 0.000 claims 2
- 230000001678 irradiating effect Effects 0.000 claims 2
- 239000011859 microparticle Substances 0.000 claims 2
- 229920005597 polymer membrane Polymers 0.000 claims 2
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- 239000007983 Tris buffer Substances 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000004043 dyeing Methods 0.000 claims 1
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical class [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 claims 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 1
- XRXJDLHDDHBJOJ-UHFFFAOYSA-N CN(C=C1)c2ccccc2/C1=C/c1[n+](C)c(cccc2)c2[s]1 Chemical compound CN(C=C1)c2ccccc2/C1=C/c1[n+](C)c(cccc2)c2[s]1 XRXJDLHDDHBJOJ-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50763003P | 2003-09-30 | 2003-09-30 | |
| PCT/US2004/032693 WO2005033342A1 (en) | 2003-09-30 | 2004-09-30 | Detection of immobilized nucleic acid |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010255930A Division JP2011099862A (ja) | 2003-09-30 | 2010-11-16 | 固定化された核酸の検出 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2007507238A JP2007507238A (ja) | 2007-03-29 |
| JP2007507238A5 true JP2007507238A5 (https=) | 2007-11-22 |
| JP4651621B2 JP4651621B2 (ja) | 2011-03-16 |
Family
ID=34421643
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006534241A Expired - Lifetime JP4651621B2 (ja) | 2003-09-30 | 2004-09-30 | 固定化された核酸の検出 |
| JP2010255930A Pending JP2011099862A (ja) | 2003-09-30 | 2010-11-16 | 固定化された核酸の検出 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010255930A Pending JP2011099862A (ja) | 2003-09-30 | 2010-11-16 | 固定化された核酸の検出 |
Country Status (7)
| Country | Link |
|---|---|
| US (7) | US7727716B2 (https=) |
| EP (2) | EP1988177A1 (https=) |
| JP (2) | JP4651621B2 (https=) |
| AT (1) | ATE428002T1 (https=) |
| CA (1) | CA2540508A1 (https=) |
| DE (1) | DE602004020484D1 (https=) |
| WO (1) | WO2005033342A1 (https=) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1988177A1 (en) | 2003-09-30 | 2008-11-05 | Molecular Probes Inc. | Detection of immobilised nucleic acid |
| US7776567B2 (en) * | 2005-03-17 | 2010-08-17 | Biotium, Inc. | Dimeric and trimeric nucleic acid dyes, and associated systems and methods |
| US7601498B2 (en) * | 2005-03-17 | 2009-10-13 | Biotium, Inc. | Methods of using dyes in association with nucleic acid staining or detection and associated technology |
| US20080057557A1 (en) * | 2006-08-31 | 2008-03-06 | Invitrogen Corporation | Methods, cassettes, gels and apparatuses for isolation and collection of biomolecules from electrophoresis gels |
| JP5086362B2 (ja) * | 2006-11-02 | 2012-11-28 | エフ．ホフマン−ラ ロシュ アーゲー | 新規ｄｓＤＮＡ結合蛍光色素 |
| EP2201009B1 (en) | 2007-10-02 | 2016-10-26 | Thermo Fisher Scientific Inc. | Monomethine dyes |
| US8877437B1 (en) | 2009-12-23 | 2014-11-04 | Biotium, Inc. | Methods of using dyes in association with nucleic acid staining or detection |
| EP2697730A4 (en) | 2011-04-15 | 2015-04-15 | Advanced Diamond Technologies Inc | ELECTROCHEMICAL SYSTEM AND METHOD FOR PROPORTION OF OXIDIZERS AT HIGH CURRENT DENSITY |
| JP2013040136A (ja) * | 2011-08-17 | 2013-02-28 | Tosoh Corp | ４−アルコキシ−２−（４−アミノスチリル）ベンゾチアゾリウム塩、その製造方法およびそれを用いる核酸の検出方法 |
| WO2014089421A1 (en) * | 2012-12-07 | 2014-06-12 | Life Technologies Corporation | Detection of rna molecules using substituted unsymmetrical cyanine dyes |
| US10011570B2 (en) | 2013-10-03 | 2018-07-03 | Biotium, Inc. | Nucleic acid binding dyes with improved safety |
| CN104004378B (zh) * | 2014-06-11 | 2015-12-09 | 南方医科大学 | 一种苯并吲哚半菁染料及其用途 |
| US9969887B2 (en) * | 2016-04-20 | 2018-05-15 | Sfc Co., Ltd. | Merocyanine-based compounds, and dyes, kits and contrast medium compositions for labelling biomolecules comprising the same |
| WO2019063795A1 (en) * | 2017-09-30 | 2019-04-04 | Bioatlas Oü | MULTI-COMPONENT TABLET FOR THE PRODUCTION OF AGAROSE GEL AND RELATED METHODS |
| CN114174799A (zh) * | 2019-05-30 | 2022-03-11 | 贝克曼库尔特有限公司 | 用于固定生物标本以进行显微成像的方法和系统 |
| US12169166B2 (en) | 2021-02-16 | 2024-12-17 | Aat Bioquest, Inc. | Fluorogenic cyanine compounds for detecting nucleic acids |
| US20230392188A1 (en) * | 2022-06-02 | 2023-12-07 | Perkinelmer Health Sciences, Inc. | Electrophoresis-mediated characterization of dna content of adeno-associated virus capsids |
| WO2024081869A1 (en) | 2022-10-14 | 2024-04-18 | 10X Genomics, Inc. | Methods for analysis of biological samples |
| WO2025125266A1 (en) * | 2023-12-11 | 2025-06-19 | Cube Biotech Gmbh | Tablets containing fuctionalized agarose particles, method for producing them and use of the tablets |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883867A (en) | 1985-11-01 | 1989-11-28 | Becton, Dickinson And Company | Detection of reticulocytes, RNA or DNA |
| US4957870A (en) * | 1985-11-01 | 1990-09-18 | Becton, Dickinson And Company | Detection of Reticulocytes, RNA and DNA |
| US5321130A (en) * | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
| US5436134A (en) | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5534416A (en) | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
| US5656449A (en) * | 1995-03-06 | 1997-08-12 | Molecular Probes, Inc. | Neutral unsymmetrical cyanine dyes |
| US7824532B2 (en) * | 1995-04-26 | 2010-11-02 | Life Technologies Corporation | Apparatus and method for electrophoresis |
| US5582702A (en) | 1995-04-26 | 1996-12-10 | Ethrog Biotechnology Ltd. | Apparatus and method for electrophoresis |
| US6379516B1 (en) | 1995-04-26 | 2002-04-30 | Ethrog Biotechnology Ltd. | Apparatus and method for electrophoresis |
| US5929227A (en) | 1995-07-12 | 1999-07-27 | The Regents Of The University Of California | Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores |
| DE69837839T2 (de) | 1997-03-07 | 2007-12-13 | Clare Chemical Research LLC, Denver | Fluorometrischer Nachweis mit sichtbarem Light |
| US5865874A (en) * | 1997-06-27 | 1999-02-02 | Duracell Inc. | Hydrogen storage alloy |
| CA2321100A1 (en) | 1998-02-19 | 1999-08-26 | Edvotek | Articles of manufacture and methods for staining biomolecules |
| US6562213B1 (en) * | 2000-08-30 | 2003-05-13 | Ethrog Biotechnology Ltd. | Electrophoresis apparatus for simultaneous loading of multiple samples |
| JP2004527739A (ja) * | 2001-03-08 | 2004-09-09 | エスログ・バイオテクノロジー・リミテッド | 電気泳動のための装置及び方法 |
| EP1988177A1 (en) | 2003-09-30 | 2008-11-05 | Molecular Probes Inc. | Detection of immobilised nucleic acid |
-
2004
- 2004-09-30 EP EP08158255A patent/EP1988177A1/en not_active Withdrawn
- 2004-09-30 CA CA002540508A patent/CA2540508A1/en not_active Abandoned
- 2004-09-30 WO PCT/US2004/032693 patent/WO2005033342A1/en not_active Ceased
- 2004-09-30 JP JP2006534241A patent/JP4651621B2/ja not_active Expired - Lifetime
- 2004-09-30 DE DE602004020484T patent/DE602004020484D1/de not_active Expired - Lifetime
- 2004-09-30 US US10/956,868 patent/US7727716B2/en active Active
- 2004-09-30 AT AT04789513T patent/ATE428002T1/de not_active IP Right Cessation
- 2004-09-30 EP EP04789513A patent/EP1668162B1/en not_active Expired - Lifetime
-
2010
- 2010-04-30 US US12/771,897 patent/US7977057B2/en not_active Expired - Lifetime
- 2010-05-12 US US12/778,774 patent/US20100227331A1/en not_active Abandoned
- 2010-11-16 JP JP2010255930A patent/JP2011099862A/ja active Pending
-
2011
- 2011-08-30 US US13/221,208 patent/US20120058566A1/en not_active Abandoned
-
2012
- 2012-06-18 US US13/525,690 patent/US20130011927A1/en not_active Abandoned
-
2013
- 2013-05-15 US US13/895,207 patent/US8969004B2/en not_active Expired - Lifetime
-
2015
- 2015-03-02 US US14/636,063 patent/US20150247799A1/en not_active Abandoned
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