JP2007261951A - Cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To solve the problem that especially when a sake is added as a cosmetic, a significant amount of this must be added so as to exhibit various cosmetic functions, and to improve it from both an aspect of cost and an aspect of compositional constitution. <P>SOLUTION: Hydrophobic compositions in the sake are efficiently concentrated in order to obtain the cosmetic with high effects of improving skin/antioxidant effectiveness/whitening effectiveness and a manufacturing method thereof. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

The present invention relates to a method for adding and recovering an adsorbent to a rice fermentation broth and extracting a hydrophobic mixture from the adsorbent, and more particularly to a cosmetic using the hydrophobic mixture as a raw material and a method for producing the same.

Brewed sake such as sake and wine contains not only various active ingredients derived from raw materials, but also contains a large amount of metabolites such as yeast, koji molds, and lactic acid bacteria. It has been known for a long time that it has the effect of improving gloss and tension. Especially for sake, there are various cosmetics that take advantage of its characteristics.

  Japanese Patent Laid-Open No. Hei 6-199679 discloses that an organic solvent soluble material obtained from fermented rice, particularly sake lees, which is a by-product of sake production, has an inhibitory effect on tyrosinase activity. Japanese Patent Application Laid-Open No. 2001-106610 includes a method for producing a cosmetic comprising fermented liquor, comprising activated charcoal-treated fermented liquor obtained by adsorbing and removing aldehydes, ketones, organic acids and the like contained in fermented liquor by activated carbon. Is disclosed. On the other hand, Japanese Patent Application Laid-Open No. 2004-245852 discloses a method for recovering a tyrosinase activity inhibitor by bringing a distillation residue of brown sugar shochu, which is distilled liquor, into contact with an adsorbent.

Japanese Patent Laid-Open No. 6-199679 JP 2001-106610 A JP 2004-2458592 A

However, the method disclosed in Patent Document 1 recovers components derived from raw materials or insolubilized during fermentation by suspending sake lees, which are by-products of sake, with ethanol. It is thought to be concentrated.
In addition, the method disclosed in Patent Document 2 is a method in which components contained in sake are adsorbed by activated carbon, but these adsorbed components are stimulating substances such as aldehydes, ketones and organic acids. It has been shown that the part effective as a cosmetic is activated charcoal-treated sake from which these adsorbing components have been removed.
Further, the method disclosed in Patent Document 3 is a method in which an active ingredient is adsorbed on activated carbon by a method similar to Patent Document 1 from a distilled spirit of brown sugar shochu, which is a distilled liquor. Unlike sake, a distilled spirit is heated for a long time. Therefore, it is highly possible that a compound originally not present in the mash is formed.
It was known that sake, a fermented liquor, has a cosmetic effect, but its active ingredients are thought to exist in the sake lees or the non-adsorbed fraction of activated carbon in sake. It is not clear.
That is, particularly when sake is added as a cosmetic, it must be added to a considerable extent in order to exhibit various beauty functions, and improvements have been demanded from the standpoint of cost and composition.
The present invention has been made in view of such problems, and efficiently concentrates a hydrophobic composition contained in sake to provide a cosmetic having a high skin improvement effect, antioxidant effect, and whitening effect, and a method for producing the same. It is something to try.

In order to solve the above-mentioned problems, the present inventor has repeatedly studied to add an adsorbent to the rice fermentation broth, and to the recovered adsorbent, a polar solvent or a nonpolar solvent (for example, chloroform, methanol, or a mixture thereof, hexane, It was found that a hydrophobic mixture obtained by adding 2 to 10 times the amount of adsorbent and stirring the mixture after removing the adsorbent by filtration or the like has the following effects.
(1) As a result of the water retention test, the 1.6% hydrophobic mixture showed a moisture retention rate superior to that of the commercially available ceramide. From this, the hydrophobic mixture has an excellent effect as a moisturizing component of cosmetics, and a sufficient skin moisturizing effect can be expected.
(2) In order to conduct an occlusive test, 5 ml of water was put into a vial equipped with a filter coated with a hydrophobic mixture, a commercial emulsion, or water, and the amount of water transpiration after 1 hour was measured. The sex mixture suppressed water transpiration to the same extent as commercial emulsions. Also from this fact, the hydrophobic mixture shows an excellent effect as a moisturizing component for cosmetics, and a sufficient skin moisturizing effect can be expected.
(3) As a result of the antioxidant test, the antioxidant power of the hydrophobic mixture was 180 times that of the rice fermentation broth, and was comparable to 1% ferulic acid when compared with ferulic acid known as an antioxidant. From this, the hydrophobic mixture can be expected to have effects such as prevention of oxidation and generation of free radicals.
(4) When tyrosinase inhibitory activity was measured, the tyrosinase inhibitory activity of the hydrophobic mixture was about 900 times that of the rice fermentation broth. Furthermore, when compared with arbutin, the inhibitory activity of the hydrophobic mixture was equivalent to 0.3% arbutin. From this, the hydrophobic mixture can also be expected to have an effect as a whitening component.
The present invention has been completed based on the above findings, and provides the following hydrophobic mixture, cosmetics and the like.
Item 1. The hydrophobic mixture which consists of the thing which collect | recovered the adsorbent added to the rice fermentation liquor, and extracted with the solvent from this adsorbent.
Item 2. Item 2. The hydrophobic mixture according to Item 1, wherein the adsorbent is activated carbon made from wood flour and / or raw ash.
Item 3. Item 2. The hydrophobic mixture according to Item 1, wherein the adsorbent is a resin.
Item 4. Item 2. The hydrophobic mixture according to Item 1, wherein the solvent is a polar solvent.
Item 5. Item 2. The hydrophobic mixture according to Item 1, wherein the solvent is a nonpolar solvent.
Item 6. Item 6. A cosmetic comprising the hydrophobic mixture according to Item 1 to 5.
Item 7. Item 7. The cosmetic according to Item 6, which has at least one of a skin improvement effect, an antioxidant effect, and a whitening effect.
Item 8. Item 8. The cosmetic according to Item 7, wherein the skin improvement effect is an improvement in water retention and / or an improvement in occlusion.
Item 9. Item 8. The cosmetic according to Item 7, wherein the antioxidant effect is prevention of oxidation and / or prevention of free radical generation.
Item 10. Item 8. The cosmetic according to Item 7, wherein the whitening effect is due to tyrosinase inhibitory activity and / or melanolytic activity.

Rice fermentation broth-derived hydrophobic mixture obtained by adding adsorbents such as activated carbon and silica gel to rice fermentation broth and stirring and extracting the components adsorbed on the adsorbent with organic solvent is moisturizing, occlusive, antioxidant, tyrosinase inhibition It has various functions such as action and can be used as a new cosmetic different from conventional rice fermentation broth.
In addition, since the raw materials have various functions (humidity retention, occlusiveness, antioxidant properties, tyrosinase inhibitory action) required for cosmetics, they can be used as excellent cosmetics derived from natural materials.

Hereinafter, the present invention will be described in detail.
(1) Rice fermented liquid The rice fermented liquid in the present invention refers to a clear liquid obtained by solid-liquid separation of fermented mash that has been fermented by adding water, rice bran, or yeast to steamed rice. The rice used as the raw material for the steamed rice is not particularly limited, and may be Japanese rice as well as overseas rice such as Thai rice. The rice may be brown rice or polished rice, and red rice bran or white rice bran may be used as a raw material. Rice bran may be added in an amount necessary for fermentation of moromi, and may be replaced by an enzyme agent such as glucoamylase in some cases. As the yeast, sake yeast is preferably used, but in some cases, yeast having a specific function such as laboratory yeast or recombinant yeast may be used.
The solid-liquid separation method may use general squeeze filtration or continuous centrifugation.
(2) Adsorbent If the adsorbent used in the present invention is a porous substance such as activated carbon, silica gel, styrene-based adsorbent, anion exchange resin, cation exchange resin, diatomaceous earth, zeolite, or synthetic polymer. Although it is good, it is preferably activated carbon in view of the sake production process.
The type of the activated carbon is not particularly limited, but is preferably made of wood flour or raw ash that is certified as a brewing material. The activated carbon activation method is preferably zinc chloride charcoal or steam charcoal activated with zinc chloride or water vapor. The shape is not particularly limited, but is preferably a fine powder from the viewpoint of efficient extraction, and is preferably granular from the viewpoint of workability during work.
(3) Hydrophobic mixture extraction method from adsorbent A polar solvent or nonpolar solvent and a mixture thereof may be used for extraction of the hydrophobic mixture contained in sake adsorbed on the adsorbent. , Ethanol, propanol, butanol, propylene glycol, butylene glycol, glycerin and other alcohols, acetone, acetonitrile, hexane, chloroform and the like.
(4) Shape of the hydrophobic mixture The hydrophobic mixture may be used as it is, but may be pulverized and concentrated by freeze drying, spray drying, concentration to dryness, or the like, if necessary.
(5) Manufacturing method The cosmetics of this invention can be manufactured by adding the hydrophobic mixture obtained by this invention to the existing cosmetics composition. The dosage form of the cosmetic composition is arbitrary, and it is a capsule, powder, granule, solid, liquid, gel, foam, emulsion, cream, ointment, sheet, mousse, powder dispersion It forms dosage forms such as multilayers and aerosols. Specifically, skin lotion, essence, whitening lotion, milk, whitening milk, cream, whitening cream, ointment, whitening ointment, lotion, whitening lotion, oil, pack and other basic cosmetics, soap, cleansing cream, cleansing Examples include skin cleansing agents such as lotions, cleansing milks, and facial cleansers, foundations, white powders, white powders, makeup cosmetics such as lipsticks, blushers, eye shadows, and eyeliners, and bath preparations. The content of the hydrophobic mixture contained in the cosmetic composition is generally used in a concentration range of 0.0001% by mass or more, preferably 0.01 to 50.0% by mass in the total amount of the composition. Particularly preferably, 0.1 to 20% by mass is effective from the viewpoint of expression of a cosmetic effect expected in constituting a cosmetic composition.
In addition to the above-mentioned compounds that are essential components, the cosmetic composition constituted using the whitening agent of the present invention further includes a pigmentation inhibitor, a tyrosinase activity inhibitor, a melanocyte melanin production inhibitor, and a humectant exemplified below. , Cell activator / metabolic activator, antioxidant, active oxygen scavenger / radical production inhibitor, fat metabolism promoter, UV protection agent / UV absorption promoter, astringent, anti-inflammatory agent / interleukin production inhibitor / Antiinflammatory agent, Antiseborrheic agent, Antibacterial agent / Antiviral agent, Blood flow promoter / Vessel stimulant, Antiandrogen agent, Structural proteolytic enzyme (elastase, collagenase, keratin protease, serine protease, integrin degrading enzyme, involucrin degradation Enzyme, filaggrin degrading enzyme, laminin degrading enzyme, fibronectin degrading enzyme, proteoglycan degrading enzyme, etc.) Harmful agents, structural protein synthesis promoters, mucopolysaccharide (hyaluronic acid, chondroitin sulfate, etc.) degrading enzyme inhibitors, mucopolysaccharide synthesis promoters, intercellular adipogenesis promoters / intercellular lipid state improvers, keratolytic agents / Stratum corneum peeling promoter, plasminogen activator antagonist inhibitor, Maillard reaction inhibitor, testosterone 5α reductase activity inhibitor / hair papilla activator / hair growth promoter, hair matrix cell growth inhibitor / hair growth inhibitor, Active ingredients such as hair swelling agents / hair protecting agents, odorous substance erasing agents, and other plant-based materials, animal-based materials, microbial-based materials, and other natural materials that are preferred for use in forming cosmetic composition forms It is possible to provide an excellent cosmetic composition having various functions by arbitrarily selecting and using ingredients such as extracts and metabolites derived from raw materials, etc., or various compounds as additives. Can be provided. The content in the preparation is not particularly defined, but usually a concentration range of 0.0001 to 50% by mass is common.

  EXAMPLES Hereinafter, although an Example is shown and this invention is demonstrated in detail, this invention is not limited to these Examples.

Method for producing hydrophobic mixture After adding 500 g of activated carbon (Takeda Kirin Foods Co., Ltd., Ginjo Hakuho 50 W) to 500 liters of sake, the mixture is stirred to adsorb hydrophobic components and injected into a pressure filter with a transfer pump while stirring. And circulatingly filtered at a filtration rate of about 2000 ml / min. As a result, the hydrophobic mixture contained in the sake is adsorbed and concentrated on the activated carbon. When the circulated sake became colorless, the filtration was terminated and the activated carbon was recovered. 3 l of a polar solvent (chloroform: methanol = 2: 1) was added to the activated carbon and stirred, followed by filtration to remove the activated carbon to obtain a polar solvent containing a solvent extract. The solvent was removed using a rotary evaporator to obtain about 20 g of a hydrophobic mixture. The hydrophobic mixture is a mixture of a solid and a liquid at normal temperature, but when used in various tests, it was heated to 50 ° C. and used as a uniform liquid.

Water retention test The water retention of the hydrophobic mixture was examined. As a control for comparison, ceramide derived from rice was used. Since the hydrophobic mixture and ceramide have low solubility in water, respective emulsions were prepared. The composition of the emulsion is as shown in Table 1.

FIG. 1 shows the result of measuring the moisture retention rate by leaving this in a constant temperature and humidity chamber under a moisture condition of 40 ° C. and a humidity of 40% and measuring the weight after 24 hours.
When the moisture retention of the control (containing neither hydrophobic mixture nor ceramide) was compared with 1, the moisture retention was higher than 1.1 when 1.6% hydrophobic mixture was used. That is, the moisture retention improvement effect of 10% or more was recognized by adding 1.6% of the hydrophobic mixture. Moreover, the value higher than the case of the commercially available ceramide used for the comparison was shown.

Occlusion test Water and emulsion were used for comparison with the hydrophobic mixture. As the emulsion, a commercially available emulsion (manufactured by Laurel Wreath Co., Ltd .; main components: water, squalane, ethanol, glycerin) was used.
Place 5 ml of water in a vial (10 ml), and install a filter (Millipore, pore size 0.45 μm) coated with 50 μl of sample or water inside the holed cap, at room temperature of 24 ° C. and humidity of 27% FIG. 2 shows the results of measuring the amount of water transpiration after 1 to 3 hours using a Tewmeter TM300 (manufactured by Integral).
In the first to third hours after the start of the experiment, the moisture transpiration when using a filter coated with water was about 16.7 to 18.6 g / hm ² , whereas the moisture when using a hydrophobic mixture transpiration rate was 9.35~9.70g / hm ^2.
On the other hand, the amount of water transpiration when using a commercially available emulsion was 11.3 to 13.0, and the hydrophobic mixture showed an occlusive property exceeding that of the emulsion throughout the test period.
From this result, it is possible to expect a moisture retention ability that suppresses evaporation of moisture from the skin of the hydrophobic mixture.

Antioxidant Test Antioxidant power was measured using DPPH (1,1-Diphenyl-2-picrylhydrazyl) method, and the antioxidant power of the hydrophobic mixture was measured using rice fermented liquid (manufactured by Gekkeikan Co., Ltd., pure rice sake), ferulic acid ( Wako Pure Chemical). Ferulic acid is a physiologically active ingredient contained in rice bran and has an antioxidant effect and is one of the ingredients blended in cosmetics to prevent the generation of free radicals.
The antioxidant test by the DPPH method was performed as follows. Add 2 ml of 50% alcohol to 0.5 ml of either hydrophobic mixture or rice fermentation liquid, ferulic acid and water, and add 0.2 ml of 2M acetate buffer (pH 5.0) and 0.3 ml of 1 mM DPPH. Was placed in the dark for 30 minutes, and OD ₅₂₀ was measured.
Table 2 shows the results obtained by measuring each of the hydrophobic mixture, the rice fermentation broth, and ferulic acid at different concentrations, and calculating the respective IC ₅₀ values from the measured values.

  As a result, the hydrophobic mixture had antioxidant properties, and the antioxidant power was almost the same as 1% ferulic acid. Moreover, the antioxidant property of the rice fermentation broth was about 1/180 of the hydrophobic mixture. From this, it was shown that the hydrophobic mixture effectively extracted and concentrated the antioxidant component in the rice fermentation broth by the treatment of the present invention.

Tyrosinase activity inhibition test The tyrosinase activity inhibitory effect of a hydrophobic mixture or rice fermentation broth, α-arbutin (manufactured by Ezaki Glico Co., Ltd.) was compared. α-Arbutin is known to exhibit a tyrosinase activity inhibitory effect that exceeds β-arbutin, which is well known as a whitening effect substance.

The tyrosinase activity inhibition test was measured as follows. Add 150 μl of 1 mM tyrosine (0.01N HCl) and 10 μl of tyrosinase (150 u / ml; derived from Aspergillus) to 150 μl of 1M phosphate buffer (pH 6.0), and add water and test solution (hydrophobic mixture or rice fermentation broth, arbutin) ) the 2.7ml of the combined added, immediately measured over time _{OD 475,} was sample concentration tyrosinase activity inhibition rate is 50% and the _{IC 50.} The results are shown in Table 3.

  The hydrophobic mixture showed an extremely high tyrosinase activity inhibitory effect (900 times or more) compared to the rice fermentation broth. This inhibitory activity corresponded to the inhibitory activity of 0.3% arbutin. From this, it was shown that the hydrophobic mixture effectively extracted and concentrated the tyrosinase activity inhibitory component in the rice fermentation broth by the treatment of the present invention.

It is the figure which compared the moisture retention of the hydrophobic mixture derived from a rice fermentation liquid, and ceramide. It is the figure which compared the obstruction | occlusion property of the hydrophobic mixture derived from water and a rice fermentation liquid, and a commercially available emulsion.

Claims (10)

The hydrophobic mixture which consists of the thing which collect | recovered the adsorbent added to the rice fermentation liquid, and extracted with the solvent from this adsorbent. The hydrophobic mixture according to claim 1, wherein the adsorbent is activated carbon made from wood flour and / or raw ash. The hydrophobic mixture according to claim 1, wherein the adsorbent is a resin. The hydrophobic mixture according to claim 1, wherein the solvent is a polar solvent. The hydrophobic mixture according to claim 1, wherein the solvent is a nonpolar solvent. Cosmetics comprising the hydrophobic mixture according to claim 1. The cosmetic according to claim 6, which has at least one function or two or more functions among a skin improvement effect, an antioxidant effect, and a whitening effect. The cosmetic according to claim 7, wherein the skin improvement effect is an improvement in water retention and / or an improvement in occlusion. The cosmetic according to claim 7, wherein the antioxidant effect is prevention of oxidation and / or prevention of free radical generation. The cosmetic according to claim 7, wherein the whitening effect is due to tyrosinase inhibitory activity and / or melanolytic activity.

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