JP2007223973A - Granular preparation for controlling scarabaeidae insect pest, method for producing the preparation, and method for controlling scarabaeidae insect pest by using the preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a granular preparation for controlling a Scarabaeidae insect pest, exhibiting high controlling effects by maintaining many spores of Bacillus popilliae having fatal activities on larvas of the Scarabaeidae insect pest, in an edible state in a soil for a long period; and to provide a method for producing the preparation, and a method for controlling the Scarabaeidae insect pest by using the preparation. <P>SOLUTION: The granular preparation for controlling the Scarabaeidae insect pest contains (1) the spore of the Bacillus popilliae, (2) a vegetable powder having properties for feeding and attracting the Scarabaeidae pest insects, and (3) at least one kind selected from the group consisting of waxes, oils and fats, oxidated product of the oils and fats, and ethylene oxide adducts thereof. The method for producing the preparation comprises kneading the components (1) to (3), granulating the kneaded product, and drying the granules at 40-80°C. The method for controlling the Scarabaeidae insect pest comprises homogeneously mixing the preparation with soil before and/or at the transplantation of crops, and spraying the mixed product at least at ≥10 cm depth from the surface of the earth. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a granular material containing spores of Bacillus popillie (hereinafter referred to as spores) for controlling various scarab beetle pests that cause great food damage in the agricultural field of producing sweet potatoes, radish, carrots, potatoes and the like. The present invention relates to a preparation, a method for producing the preparation, and a method for controlling Scarabaeidae pests using the preparation.

  Scarabaeidae larvae that feed on various crops and horticultural plants, especially Douganebububu, Semadara-kogane, Japanese beetle, hime-kogane, etc., in vegetable fields, farms, orchards, gardens, forests, greenhouses, grasslands, pastures and other horticultural facilities Are known as pests that cause feeding damage to the roots (tubers) of crops and horticultural plants and cause serious damage to their growth. In recent years, the poor quality of crops and the reduction of production due to the occurrence of damage caused by tubers of root vegetables such as sweet potato, potato, carrot and radish, which are caused by these pests, have become a major problem.

  For these scarab beetle larvae, conventionally, wettable powders of synthetic insecticides, especially organophosphorus pesticides (for example, diazinon, fenthion, isoxathion, etc.) and carbamate pesticides (for example, benfuracarb, carbosulfan, mesomil, etc.) Suspensions in water (sols) have been used by application of soil mixing, soil surface treatment, and foliage spraying. However, in the crop cultivation area, the above-mentioned Scarabaeidae pests perform harming behavior in the soil, so that it is difficult for the pesticides to reach the larvae-growing environment in the soil by spraying the chemical from the ground. In many cases, suspension blending with soil causes problems with the stability of the formulation itself, decomposition of the active ingredient in the soil, and lack of control effect due to larval repellent behavior. Has been.

  As a method for solving these problems, multiple pesticides can be dropped, but application of pesticides after planting of crops not only requires a lot of labor, but the effects are also fully demonstrated for the above reasons. It was often difficult. In addition, large amounts of these synthetic pesticides are often applied with increased soil residues, and depending on precipitation and soil quality, there is a risk of contamination in groundwater and river water.

  On the other hand, as a control technique using parasitic microorganisms, microorganisms belonging to Bacillus sporiye that have been isolated from larvae of Japanese beetles, alien insects from Japan, were already emulsified in the United States in 1933 with respect to larvae of Scarabaeidae. It was known to induce disease (see, for example, Non-Patent Document 1). However, in an actual field test, there was no effect on the moth buoy (see, for example, Non-Patent Document 2).

  In addition, there is a report (see Non-Patent Document 3) in which spore of artificially cultured Bacillus poppyrie is diluted in water and sprayed from turfgrass (see Non-Patent Document 3), but in the case of turfgrass where larvae survive a few centimeters from the ground surface There is no report as a means of controlling Scarabaeidae pests of root vegetables where larvae survive at a depth of several tens of centimeters from the surface.

In addition, a microorganism belonging to Bacillus thuringiensis has been reported as a microorganism having an insecticidal effect against the stag beetle and the white squirrel (see Patent Document 1). Although the wettable powder and suspending agent containing spore or spore of the microorganisms collectively called BT agent have been registered as agricultural chemicals, the control effect is unstable due to the influence of ultraviolet rays when sprayed. It is considered that the current situation is that it is hardly spread (for example, see Non-Patent Document 2).
JP-A-8-228783 Walter E.M. Fleming, (1968) Biological Control of the Japan Beetles. US. Department Agricultural Technical Bulletin 1383. Washington D.C. C Agricultural Microorganisms-Their Use and Prospects-Umedani Seiji, Kato Satoshi 236 (1990) DIC Technical Review No. 9 2003 Agriculturally useful microorganisms-their utilization and prospects-Umedani, Seiji, Kato, Satoshi (1990)

  In order for the microorganisms belonging to Bacillus popilli known as an emulsifying disease fungus to exert a sufficient control effect against Scarabaeidae pests in an actual field, it is necessary to optimize the formulation in consideration of the cultivation system and insect ecology. Regarding the cultivation of tuber crops such as sweet potatoes and potatoes, seedlings are planted from late April to mid-May, growing in the hot summer season, and tubers are harvested from September to October. During this period, adult beetles fly from June to August during the high temperature period and lay eggs in the soil. After hatching, the larvae move through the soil as they grow from 1 to 3 years old, and are growing. Will cause damage to the tubers. Therefore, unless it is a formulation type that acts effectively during this period, a sufficient control effect cannot be achieved in an actual field. In addition, if a large amount of spores are not taken into the larvae, emulsification disease cannot be induced in the larvae. When a preparation such as a wettable powder or suspension in water containing only Bacillus poppyrie spores as an active ingredient is diluted with water and mixed with the soil before planting, a sufficient amount of spores necessary for control are effectively taken up by the larvae. As a result, the insecticidal effect was limited. In addition, even if soil mixing treatment using a granular preparation containing Bacillus sporije spores using a conventional formulation technique for agrochemical granular preparations, the larvae do not eat, and the preparation easily disintegrates in the soil. As a result, it was difficult to effectively act on larvae. In addition, there is a problem that it is necessary to apply many times over the long period when the crop grows.

  The object of the present invention is to provide a method for controlling Scarabaeidae pests such as Douganebububu, Sedarae, Himekogane, and Japanese beetle, and these pests are highly species-specific, selectively parasitic, and cause emulsification disease due to rapid bioproliferation. Pre-planting is achieved by allowing spore of Bacillus popilie, which exhibits larval lethal activity, to exist in a stable form for a long time in the soil and maintain a large amount of spore that is feeding-inducing and ingestible. And / or a novel granular formulation for controlling pest control insects that can reliably exert a high control effect with a small number of soil admixing treatments (single treatment) at the time of planting, a method for producing the preparation, and a chafer using the preparation The object is to provide a method for controlling pests.

  As a result of intensive studies, the present inventors have included a necessary amount of spores of Bacillus popilli that specifically parasitize a wide range of Scarabaeidae pests and have high larval lethal activity in a substrate that has feeding incentive properties, And it discovers that the said subject can be solved by containing the component which has water repellency in order to maintain this in the form which can be eaten stably over a long period of time in soil, and completes this invention. It came to.

  That is, the first invention of the present invention comprises (1) Bacillus popillie spores, (2) a plant powder having a feeding incentive to a scarab beetle, (3) waxes, A granular preparation for controlling pest control insects, comprising at least one selected from the group consisting of oils and fats, oxides of fats and oils and ethylene oxide adducts thereof.

  The second invention of the present invention is a method for producing a granular preparation for controlling pestidae pests according to the first invention, comprising: (1) Bacillus popillie spores; and (2) Scarabaeidae. A plant powder having a feeding-inducing effect on pests, and (3) at least one selected from the group consisting of waxes, fats and oils, oxides of waxes and fats and ethylene oxide adducts thereof, and After kneading, the kneaded product is granulated, and the granulated product is dried at 40 to 80 ° C.

  Further, the third invention of the present invention is a granular preparation for controlling pestidae pests according to the first invention, which is uniformly mixed with soil before planting and / or planting the target crop at least 10 cm or more from the ground surface. It is a method for controlling a scarab beetle pest, characterized by being sprayed to a depth of.

  According to the present invention, a granular formulation for controlling pest control insects that can maintain the granular formulation shape for a long period of time without being disintegrated by soil moisture, soil microorganisms, or the like, unlike ordinary agrochemical granular formulations. By using this preparation, adult larvae that irregularly fly during the growing season of the crop, for example from May to October, lay eggs in the soil. There are many opportunities to come into contact with ingestible granular formulations that are densely scattered, and Bacillus poppylier spores are more reliably taken up by larvae, thereby exerting a high larvicidal effect over a long period of time, thereby causing damage to crops. Can be prevented. In addition, since this preparation exerts the maximum control effect two to three months after spraying, for example, if it is sprayed before and / or at the time of planting in April to May, Scarabaeidae pests fly from June to August. When the adult larvae lay eggs and they hatch and become larvae, they exhibit a particularly high control effect.

The present invention will be described in detail below.
(Formulation composition)
The granular preparation for control of scarab beetles of the present invention (hereinafter sometimes abbreviated as "preparation") includes: (1) spores of Bacillus popilli, (2) plant powder having an incentive to feed against scarab beetles and (3) It contains at least one selected from the group consisting of waxes, fats and oils, oxides of waxes or fats and their ethylene oxide adducts as essential components. Among these, (1) Spore of Bacillus popilli is a component that is specifically ingested by larvae of Scarabaeidae pests and causes emulsification disease. (2) Vegetable powder is Scarabaeidae in combination with the spore. (3) Waxes, oils and fats are components that suppress degradation of the preparation by soil moisture and soil microorganisms due to their water repellent action.
In addition to these essential components, the formulation of the present invention includes, as optional components, substances used in general agrochemical granular formulations, such as binders, diluents, granulation accelerators, preservatives, and the like. It is possible to add in the range which does not impair the function.

(Specification of formulation composition)
Especially, the number of spores of Bacillus popirie contained in 1 g of the preparation is preferably 10 ^{6 to 15} / g, and the vegetable powder is preferably 1 to 90 parts by weight, more preferably 100 parts by weight of the preparation. Is 20 to 60 parts by mass, and at least one selected from the group consisting of waxes, fats and oils, oxides of waxes or fats and ethylene oxide adducts thereof is preferably 1 to 90 parts by mass, more preferably 5 to 50 parts by mass.

(spore)
Spores of Bacillus popilie are specifically ingested by the larvae of Scarabaeidae, such as Douganebububu, Semadarae, Himekogane, and Japanese beetle, to cause emulsification disease. It may be any of those collected from the above, or those obtained by artificial culture. The method of artificial culture is not particularly limited, and a conventionally known method may be applied. The obtained spores can be used either in a state containing a culture medium containing bacterial cells or the like and washed with sterilized water. Furthermore, these may be in the form of a powder dried by freeze drying or other methods.

(Amount of spores)
The number of spores of Bacillus popirie contained in 1 g of the preparation of the present invention is preferably 10 ^{6 to 15} / g, more preferably 10 ^{8 to 11} / g.

(Vegetable powder)
The plant-based powder used in the present invention has a feeding-inducing property against Scarabaeidae pests. Preferable vegetable powders include at least one powder selected from the group consisting of cereals, millet, buckwheat, legumes, potatoes and root vegetables. Rice, wheat, barley, oats, rye, etc. for millet, millet, millet, millet, sorghum, corn, etc. for millet, soybean, green beans, pea, broad bean, cowpea, azuki bean, mung bean, groundnut, etc. Potatoes include potato, sweet potato, cassava, taro, taro, konjac and the like, and root vegetables include turnip, radish, carrot, burdock and the like, but are not necessarily limited thereto.

  In the present invention, for example, when seeds or tubers are used, the plant matter can be dried and pulverized without removing or removing the outer shell or outer shell. In addition to these, these secondary products, for example, rice bran, rice husk, bran, buckwheat husk, okara, peanut husk, etc., can be used in powder form.

(Waxes, fats, etc.)
The waxes, fats and oils, waxes or fats and oxides thereof and the ethylene oxide adducts used in the present invention are preferably water-repellent and have no larval repellent effect.

(Wax)
The waxes used in the present invention are classified into natural waxes and synthetic waxes.

(Natural waxes, oxides of natural waxes and their ethylene oxide adducts)
As natural waxes, for example, paraffin wax, microcrystalline wax, petrolatum wax, carnauba wax, montan wax, candelilla wax, wood wax, rice wax, ceresin, beeswax, whale wax, lanolin and rosin are preferable. In the present invention, oxides of these natural waxes, or ethylene oxide adducts of natural waxes or oxides of natural waxes can also be used.
Among these, carnauba wax and candelilla wax are preferable because they are stable in soil for a long period of time and exhibit an excellent insecticidal effect as a preparation.

(Synthetic waxes, oxides of synthetic waxes and their ethylene oxide adducts)
Examples of the synthetic waxes include polyethylene wax and polypropylene wax. Preferred examples of the synthetic wax include oxides of these synthetic waxes, or synthetic waxes or oxides of synthetic waxes. Ethylene oxide adducts can also be used.

(Oils and fats, oxides of fats and oils and ethylene oxide adducts thereof)
Examples of the oils and fats include, but are not limited to, coconut oil, beef tallow, jojoba oil, castor oil, hydrogenated castor oil, rapeseed oil, sesame oil, corn oil, and natural fatty acids. is not. Further, in the present invention, oxides of these fats and oils, or ethylene oxide adducts of fats and oils or fats and oxides can also be used.

(Shapes such as waxes and fats)
The waxes, fats and oils, oxides of waxes and fats, and ethylene oxide adducts thereof may be used in the form of fine powder, or may be used as a water-dispersed or emulsified liquid.

(Carrier)
Conventional granular pesticide preparations are mainly composed of mineral fine powders such as bentonite, kaolin, clay, diatomaceous earth, calcium carbonate, various clay minerals, and dry plant powders such as walnut husks and corn husks. However, there is no known granular preparation containing a component having a feeding-inducing property against pests such as scarab beetles. On the other hand, the preparation of the present invention contains the essential components, so that it not only has a feeding-inducing property against Scarabaeidae pests, but can also maintain a granular preparation shape for a long period in soil.

(Binder)
Examples of the binder include CMC (carboxymethyl cellulose) and derivatives thereof, PVA (polyvinyl alcohol) and derivatives thereof, lignin sulfonate, polyacrylate, vinyl acetate-acrylate copolymer, ethylene, or styrene-acetic acid. A vinyl copolymer, a styrene-acrylate copolymer, an ethylene-maleic acid copolymer, or the like can be used as necessary.

(Diluent)
As diluent, mineral powder such as bentonite, clay, kaolin, calcium carbonate, meteorite, diatomaceous earth, pyrophyllite, porcelain stone, etc. are used as necessary. be able to.

(Granulation accelerator)
As a granulation accelerator, what is used for a normal agrochemical granular formulation, such as various nonionic surfactants and anionic surfactants, can be used as needed.

(Other additives)
In addition, as other additives, those used for usual agrochemical granular preparations such as preservatives, colorants, pH adjusters and the like can be used as necessary.

(Shape of granular preparation)
The granular preparation according to the present invention preferably has a maximum diameter of 0.2 to 20 mm. Specifically, spherical granules having an average particle size of 0.2 to 3 mm, rod-like granules having a particle size of 0.2 to 3 mm and a length of 0.3 to 20 mm, and atypical particles having a size within these ranges An agent can be mentioned as a preferable thing. If the maximum diameter is too small, the stability of the formulation in the soil will be poor, and if the maximum diameter is too large, the number of formulation particles in the soil will be too small and contact with the larvae when the application rate per unit area is constant. In some cases, the frequency decreases and the control effect is insufficient.
Examples of the shape of the preparation of the present invention, including these, include those classified into a kneading type, an adsorption type, a surface coating type, a crushing type, and the like according to the production method. When designing an actual granular formulation, it is preferable to design considering the formulation shape with high control effect, formulation productivity, formulation stability at the time of spraying when using a spraying machine, etc. .

(Production method)
The preparation of the present invention comprises (1) a spore of Bacillus popirie, (2) a vegetable powder having a feeding-inducing effect on a beetle pest, and (3) a wax, an oil or fat, a wax or an oil or fat. After kneading at least one selected from the group consisting of oxides and their ethylene oxide adducts, the kneaded product is granulated, and the granulated product is dried at 40 to 80 ° C. be able to.
The kneading may be performed according to a conventionally known method. For example, the essential component and, if necessary, the optional component or water are sufficiently kneaded using a kneader.
The granulation may be performed by, for example, a spray drying granulation method, a fluidized bed granulation method, a bread type granulation method, an extrusion granulation method, a crushing granulation method, or the like. The drying temperature at the time of drying may be set in consideration of the granulation method to be applied, but from the viewpoint of stably maintaining spores by distributing an appropriate amount of water-repellent components on the surface of the spore and the preparation surface. It is preferable to set it as 60 degreeC. For example, in the spray-drying granulation method, the drying time can be as short as about 10 minutes or less, so the upper limit of the drying temperature may be 80 ° C. However, if it takes a long time to dry, the upper limit of the drying temperature Is preferably 60 ° C.

(Pest control method of scarab beetles)
The formulation of the present invention is uniformly mixed with the soil before and / or when the target crop is planted, and the mixture is sprayed to a depth of at least 10 cm from the ground surface, thereby effectively controlling the scarab beetles. Can do.
The preparation of the present invention is produced by the above-described production method so that an appropriate amount of water-repellent components such as waxes and fats and oils are distributed on the surface of the formulation. It keeps the shape of the preparation by suppressing the disintegration of the preparation by microorganisms for a long time, and stably keeps the spores of Bacillus sporiye. Then, the formulation gradually collapses and becomes more suitable for feeding by the larvae of Scarabaeidae pests. In general, it takes about two months for the scarab beetles to fly and lay eggs in the soil before hatching. Therefore, it is preferable to design the formulation shape in consideration of the spraying time of the formulation and the time when the larvae of Scarabaeidae pests appear in the soil. However, the formulation of the present invention can be used before and / or planting the target crop. Occasionally evenly mixed with the soil and sprayed, it will remain stable in the soil until hatching, and when the hatched larvae start feeding, the decomposition of the preparation has begun gradually, A large amount of Bacillus popilie spores are ingested by larvae. Therefore, once sprayed, the preparation of the present invention does not require additional spraying and exhibits an excellent control effect over a long period of time.
In addition, as in the past, the method of spraying the spore alone or diluted with water or the like and spraying it on the soil does not provide a controlling effect, and the preparation of the present invention is applied to a depth of at least 10 cm from the ground surface. By mixing and dispersing uniformly with soil, an excellent control effect is exhibited even at such depths.

(Spreading amount, application rate)
The amount of application when the preparation of the present invention is uniformly mixed with the soil and sprayed is preferably 5 to 50 kg, more preferably 10 to 20 kg per 10a (R). Moreover, the number of granules of the preparation in the mixture of the preparation and the soil is preferably 500 to 3000, more preferably 1000 to 2000 in 1 g of the mixture.

(Target crop)
The target crops to which the present invention is applied are all crops that are the target of feeding damage by Scarabaeidae pests, and are not particularly limited. In recent years, the preparation of the present invention exhibits an excellent larval control effect against sweet potatoes, potatoes, carrots, radish, taros, and other potatoes and root vegetables, which have been particularly devastating to the damage caused by food damage, such as Douganebububu, Sedara-kogane, Himekogane, and Japanese beetle.

  Hereinafter, the present invention will be described in more detail with reference to specific examples. However, the present invention is not limited to the following examples.

(Examples 1-30 and Comparative Examples 1-16)
◎ Manufacture of test granular preparation sample 1 g of lyophilized powder (number of spores; 2 × 10 ¹² / g) of Bacillus poppylier spores obtained by culturing in an artificial medium, various plant powders or clays described in Tables 1 and 2 (Addition of clay if no plant powder is added) 50 g, bentonite 30 g, lauryl sulfate sodium salt 0.5 g are added and mixed using a mixer, then transferred to a kneader, and in Tables 1 and 2 20 g of the various waxes, oils and clays described (adding clay when no waxes or oils are added) as dry matter was added and mixed. To this was added 0.5 g of preservative (manufactured by Arch Chemical Co., Ltd .; Proxel GXL), an appropriate amount of tap water was added, kneaded well, and granulated using a simple extrusion granulator at about 50 ° C. After drying for 15 minutes, sizing and sieving were performed to obtain a test granular preparation sample having an average particle size of 0.8 mm and an average length of 2 mm.

◎ Pot test Next, 200 mg of the test granular preparation sample, 50 g of humus soil, and 4 kg of kuroboku soil were uniformly mixed and added to a magnetic pot having a diameter of 20 cm and a depth of 30 cm with 5 cm of river sand at the bottom. The test pot thus prepared was stored in a thermostatic chamber at 25 ° C. for 60 days while occasionally sprinkling water to avoid drying the soil. After storage for 60 days, a 5 cm deep hole was dug in the center of the pot, and 10 first-instar larvae immediately after hatching were placed, lightly plugged with surrounding soil, and stored for another 2 weeks. After storage for 2 weeks, the soil was removed from the pot, and the number of surviving larvae was counted. The pot test was carried out in duplicate, and the insecticidal effect of the test granular preparation sample was determined based on the insecticidal rate against the untreated without the test granular preparation sample (insecticidal rate = (number of dead dovetail larvae) / (no treatment) Average number of live larvae) × 100 (%)). The obtained results are shown in Tables 1 and 2.

(Example 31)
Using a mixer, 10 g of lyophilized powder (number of spores: 3 × 10 ¹² / g) of Bacillus poppyri spores obtained by culturing in an artificial medium, 1.4 kg of soybean powder, 945 g of bentonite, and 15 g of sodium lauryl sulfate are used. After mixing, the mixture was transferred to a kneader and 600 g of lanolin was added and mixed. 30 g of preservative (manufactured by Arch Chemical Co., Ltd .; Proxel GXL) and 350 g of tap water were added and kneaded well, followed by extrusion granulator (manufactured by Paulex Co., Ltd .; Dome Gran (trade name), screen pore size; 6 mm), dried at about 50 ° C. for 30 minutes, and sized and sieved to obtain 2.5 kg of a test granular preparation sample having an average particle size of 0.6 mm × an average length of 2 mm.

(Example 32)
10 g of freeze-dried powder (spore number: 3 × 10 ¹² / g) of Bacillus poppyri spores obtained by culturing in an artificial medium, 1.4 kg of okara powder, 1.045 kg of bentonite, 15 g of sodium lauryl sulfate, After mixing, the mixture was transferred to a kneader and 500 g of hardened castor oil was added and mixed. 30 g of preservative (manufactured by Arch Chemical Co., Ltd .; Proxel GXL) and 350 ml of tap water were added and kneaded well, followed by extrusion granulator (manufactured by Paulex Co., Ltd .; Dome Gran (trade name), screen pore size; 6 mm), dried at about 50 ° C. for 30 minutes, and sized and sieved to obtain 2.4 kg of a test granular preparation sample having an average particle diameter of 0.6 mm × an average length of 2 mm.

(Example 33)
10 g of freeze-dried powder (spore number: 3 × 10 ¹² / g) of Bacillus poppyri spores obtained by culturing in an artificial medium, 1.3 kg of bran powder, 1.245 kg of bentonite, 15 g of sodium lauryl sulfate, After using and mixing, the mixture was transferred to a kneader, and 1 L of carnauba wax 40% emulsion was added and mixed. 30 g of preservative (manufactured by Arch Chemical Co., Ltd .; Proxel GXL) was added and kneaded well, and then an extrusion granulator (manufactured by Paulex Co., Ltd .; Dome Gran (trade name), screen hole diameter; 0.6 mm) was used. And granulated and sieved to obtain 2.5 kg of a test granular preparation sample having an average particle size of 0.6 mm and an average length of 2 mm.

(Test method 1 and results)
A sweet potato field test was performed using the three test granular preparation samples produced in Examples 31-33. In the test, a test area of 4 m × 7 m was divided into a preliminarily cultivated field, and 280 g of the test granular preparation sample was evenly sprayed on the soil surface of the test area, and erection was performed while mixing with a large tractor. After planting, sweet potato (Beniotome) seedlings were planted the next day. Four and a half months after planting, we dig up the sweet potatoes in the 4m portion of the basin located in the center of each test zone, and after washing with water, investigated the number of damaged potatoes.

As a comparative test, in addition to the untreated group in which no drug treatment was performed, 10 g of lyophilized powder (number of spores: 3 × 10 ¹² / g) of Bacillus popirie was added to 15 g of sodium lauryl sulfate and lignin sulfonic acid. Talc 1 10 g of lyophilized powder of Bacillus popiris (spore count; 3 × 10 ¹² pcs / g), mixed and ground with 15 g of sodium and 260 g of diatomaceous earth and sprayed with a predetermined amount of 300-fold diluted solution with water .75 kg, bentonite 1.2 kg, mixed with 15 g of sodium lauryl sulfate, kneaded with 350 ml of tap water, extruded granulator (made by Paulex; Dome Gran (trade name), screen pore size: 0.6 mm) , And after drying at about 50 ° C. for 15 minutes, add a control group 2 in which a predetermined amount of the granulated granulated product is sprayed and added to the test group. Soil incorporation as, ribbing, conducted a survey of the damage potato number but was carried out planting. The obtained results are shown in Table 3.

  Test groups 1 to 3 sprayed with a granular preparation for controlling pest control insects of the present invention, compared to the untreated group and the control group sprayed with a spore wettable powder or spore granular preparation containing no plant powder and waxes, etc. It was found that the damage caused by pests was greatly reduced.

(Test method 2 and results)
Using the test granular preparation samples prepared in Examples 21, 25, 18 and 23 and the test granular preparation sample prepared in Comparative Example 6 (see Table 4), insecticide over time under room conditions A pot test was conducted to confirm the effect. That is, 150 mg of the test granular preparation sample (the number of spores: 2 × 10 ¹⁰ / g) was sufficiently mixed with 500 g of humus soil and 3.5 kg of kuroboku soil, and 20 g of the sample was 6 cm in diameter and 4 cm in depth. It was transferred to 100 plastic cups, covered with a lid so as not to dry, and further placed in a polystyrene foam box and stored in a thermostatic chamber at 25 ° C. Take out 20 cups for each sample on the 33rd, 46th, 60th, and 90th days from the start of storage, and place one instar larvae in each cup and raise them in a 20 ° C incubator. The mortality rate up to the 5th week was determined every week. Then, the relationship between the highest number of death rates up to the fifth week (maximum cumulative mortality rate) and the number of storage days was determined. The results are shown in FIG.
That is, the sample of Comparative Example 6 had already reduced the insecticidal effect 33 days after the start of storage, and did not show any insecticidal effect at 60 days. On the other hand, the preparations of Examples 21, 25, 18 and 23 of the present invention all showed a high insecticidal effect even after elapse of 33 days, 46 days, and even 60 days from the start of storage, and existed in the soil for a long time. Even so, it was confirmed that the insecticidal effect of the scarab beetle larvae can be maintained.

  The granular preparation for control of scarab beetles of the present invention is extremely useful as a control agent for various scarab beetles that cause great damage to agricultural products.

It is a graph which shows the relationship between the pot storage days and the maximum cumulative mortality of the scarab beetle larva when the test granular preparation samples of Examples 21, 25, 18 and 23 and Comparative Example 6 are used.

Claims (9)

(1) Bacillus popillie spores;
(2) a vegetable powder having a feeding-inducing property against a scarab beetle,
(3) at least one selected from the group consisting of waxes, fats and oils, waxes or oxides of fats and oils, and ethylene oxide adducts thereof;
A granular preparation for controlling a scarab beetle pest, comprising:   2. The granular preparation for controlling pests of papaveraceae according to claim 1, wherein the vegetable powder is at least one powder selected from the group consisting of cereal grains, millet, buckwheat, legumes, potatoes and root vegetables.   The waxes include paraffin wax, microcrystalline wax, petrolatum wax, carnauba wax, montan wax, candelilla wax, wood wax, rice wax, ceresin, beeswax, whale wax, lanolin, pine resin, polyethylene wax and polypropylene wax. The granular preparation for controlling pest control insects according to claim 1 or 2, which is at least one selected from the group.   The oil or fat is at least one selected from the group consisting of palm oil, beef tallow, jojoba oil, castor oil, hardened castor oil, rapeseed oil, sesame oil, corn oil and natural fatty acids. The granular preparation for controlling a scarab beetle pest as described. The number of spores of Bacillus popillie contained in 1 g of the preparation is 106 to ¹⁵ / g, and in 100 parts by weight of the preparation, 1 to 90 parts by weight of the vegetable powder, waxes, fats and oils And at least one selected from the group consisting of oxides of waxes or fats and fats and ethylene oxide adducts thereof is 1 to 90 parts by mass, for controlling pests of insects of Claims 1 to 4 Granular formulation.   The granular preparation for controlling pest control insects according to any one of claims 1 to 5, wherein a maximum diameter of the granular preparation is 0.2 to 20 mm. A method for producing a granular preparation for controlling pestidae pests according to any one of claims 1 to 6,
(1) Bacillus popillie spores;
(2) a vegetable powder having a feeding-inducing property against a scarab beetle,
(3) at least one selected from the group consisting of waxes, fats and oils, waxes or oxides of fats and oils, and ethylene oxide adducts thereof;
After kneading | mixing, this kneaded material is granulated, and this granulated material is dried at 40-80 degreeC, The manufacturing method of the granular preparation for the control of a scarab beetle pest characterized by the above-mentioned.   The granular preparation for controlling pest control insects according to any one of claims 1 to 6 is uniformly mixed with soil before spraying and / or planting the target crop at a depth of at least 10 cm or more from the ground surface. A method for controlling a scarab beetle pest, characterized by comprising: The method for controlling a scarab beetle pest according to claim 8, wherein the amount of the granular preparation for controlling the scarab beetle is 5 to 50 kg per 10a.

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