JP2007174957A5 - - Google Patents

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Publication number

JP2007174957A5

JP2007174957A5 JP2005376390A JP2005376390A JP2007174957A5 JP 2007174957 A5 JP2007174957 A5 JP 2007174957A5 JP 2005376390 A JP2005376390 A JP 2005376390A JP 2005376390 A JP2005376390 A JP 2005376390A JP 2007174957 A5 JP2007174957 A5 JP 2007174957A5

Authority

JP

Japan

Prior art keywords

hyaluronic acid

protein

yeast

dna encoding

synthase activity

Prior art date

2005-12-27

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

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• KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims 15
• 229920002674 hyaluronan Polymers 0.000 claims 15
• 229960003160 hyaluronic acid Drugs 0.000 claims 15
• 238000004519 manufacturing process Methods 0.000 claims 10
• 108090000623 proteins and genes Proteins 0.000 claims 10
• 102000004169 proteins and genes Human genes 0.000 claims 10
• 240000004808 Saccharomyces cerevisiae Species 0.000 claims 9
• 239000013598 vector Substances 0.000 claims 9
• 230000000694 effects Effects 0.000 claims 8
• 239000002773 nucleotide Substances 0.000 claims 6
• 125000003729 nucleotide group Chemical group 0.000 claims 6
• 101710128038 Hyaluronan synthase Proteins 0.000 claims 4
• 230000001131 transforming effect Effects 0.000 claims 3
• 241000219195 Arabidopsis thaliana Species 0.000 claims 2
• 241000701248 Chlorella virus Species 0.000 claims 2
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
• LFTYTUAZOPRMMI-CFRASDGPSA-N UDP-N-acetyl-alpha-D-glucosamine Chemical compound O1[C@H](CO)[C@@H](O)[C@H](O)[C@@H](NC(=O)C)[C@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 LFTYTUAZOPRMMI-CFRASDGPSA-N 0.000 claims 2
• LFTYTUAZOPRMMI-UHFFFAOYSA-N UNPD164450 Natural products O1C(CO)C(O)C(O)C(NC(=O)C)C1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 LFTYTUAZOPRMMI-UHFFFAOYSA-N 0.000 claims 2
• HSCJRCZFDFQWRP-UHFFFAOYSA-N Uridindiphosphoglukose Natural products OC1C(O)C(O)C(CO)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-UHFFFAOYSA-N 0.000 claims 2
• HDYANYHVCAPMJV-UHFFFAOYSA-N Uridine diphospho-D-glucuronic acid Natural products O1C(N2C(NC(=O)C=C2)=O)C(O)C(O)C1COP(O)(=O)OP(O)(=O)OC1OC(C(O)=O)C(O)C(O)C1O HDYANYHVCAPMJV-UHFFFAOYSA-N 0.000 claims 2
• 238000012258 culturing Methods 0.000 claims 2
• 239000013604 expression vector Substances 0.000 claims 2
• 230000001939 inductive effect Effects 0.000 claims 2
• 238000003259 recombinant expression Methods 0.000 claims 2
• HDYANYHVCAPMJV-USQUEEHTSA-N udp-glucuronic acid Chemical group O([P@](O)(=O)O[P@](O)(=O)OC[C@H]1[C@@H]([C@H]([C@@H](O1)N1C(NC(=O)C=C1)=O)O)O)[C@H]1O[C@@H](C(O)=O)[C@H](O)[C@@H](O)[C@@H]1O HDYANYHVCAPMJV-USQUEEHTSA-N 0.000 claims 2
• 238000000034 method Methods 0.000 claims 1
• 239000011780 sodium chloride Substances 0.000 claims 1
• 230000009466 transformation Effects 0.000 claims 1