JP2007125011A - ポリメラーゼ連鎖反応の助けによりdna又はrnaの特定のフラグメントを検出する方法 - Google Patents

ポリメラーゼ連鎖反応の助けによりdna又はrnaの特定のフラグメントを検出する方法 Download PDF

Info

Publication number
JP2007125011A
JP2007125011A JP2006289339A JP2006289339A JP2007125011A JP 2007125011 A JP2007125011 A JP 2007125011A JP 2006289339 A JP2006289339 A JP 2006289339A JP 2006289339 A JP2006289339 A JP 2006289339A JP 2007125011 A JP2007125011 A JP 2007125011A
Authority
JP
Japan
Prior art keywords
dna
pcr
primer
dye
fluorescent dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2006289339A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007125011A5 (enExample
Inventor
Alexei Viktorovich Chemeris
ヴィクトロヴィッチ チェメリス アレクセイ
Yury Mikhailovich Nikonorov
ミハイロヴィッチ ニコノロフ ユリー
Maiya Leonidovna Romanenkova
レオニードヴナ ロマネンコワ マイヤ
Dmitry Alexeevich Chemeris
アレクセーヴィッチ チェメリス ドミトリー
Ravil Rinatovich Garafutdinov
リナトヴィッチ ガラフトヂノフ ラビル
Roza Azatovna Magazova
アザトヴナ マガゾワ ローザ
Grigory Vladimirovich Maleev
ウラジミロヴィッチ マレーエフ グリゴリー
Vener Absatarovich Vakhitov
アブサタロヴィッチ ヴァヒトフ ヴェネル
Raif Gayanovich Vasilov
ガヤノヴィッチ ヴァシロフ ライフ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INST BIOKHIMII I GENETIKI UFIMSKOGO NAUCHNOGO TSENTRA RAN
Original Assignee
INST BIOKHIMII I GENETIKI UFIMSKOGO NAUCHNOGO TSENTRA RAN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INST BIOKHIMII I GENETIKI UFIMSKOGO NAUCHNOGO TSENTRA RAN filed Critical INST BIOKHIMII I GENETIKI UFIMSKOGO NAUCHNOGO TSENTRA RAN
Publication of JP2007125011A publication Critical patent/JP2007125011A/ja
Publication of JP2007125011A5 publication Critical patent/JP2007125011A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2006289339A 2006-03-01 2006-10-25 ポリメラーゼ連鎖反応の助けによりdna又はrnaの特定のフラグメントを検出する方法 Pending JP2007125011A (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2005132940/13A RU2005132940A (ru) 2006-03-01 2006-03-01 Способ детекции специфических фрагментов днк или рнк с помощью полимеразной цепной реакции в режиме реального времени

Publications (2)

Publication Number Publication Date
JP2007125011A true JP2007125011A (ja) 2007-05-24
JP2007125011A5 JP2007125011A5 (enExample) 2007-08-02

Family

ID=37529272

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2006289339A Pending JP2007125011A (ja) 2006-03-01 2006-10-25 ポリメラーゼ連鎖反応の助けによりdna又はrnaの特定のフラグメントを検出する方法

Country Status (4)

Country Link
US (1) US8198026B2 (enExample)
EP (1) EP1780291A1 (enExample)
JP (1) JP2007125011A (enExample)
RU (1) RU2005132940A (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010539940A (ja) * 2007-09-28 2010-12-24 スリーエム イノベイティブ プロパティズ カンパニー 核酸検出の二重オリゴヌクレオチド法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7393646B2 (en) 2005-06-20 2008-07-01 Cargill, Inc. Method, apparatus and system for quantifying the content of genetically modified material in a sample
RU2413770C2 (ru) * 2007-06-14 2011-03-10 Институт биохимии и генетики Уфимского научного центра РАН Способ проведения полимеразной цепной реакции с помощью конвекции
ES2595489T3 (es) * 2008-07-18 2016-12-30 Trovagene, Inc. Métodos para la detección de secuencias de ácidos nucleicos "ultracortos" basados en PCR
US20100159452A1 (en) * 2008-12-22 2010-06-24 Roche Molecular Systems, Inc. Method For Detecting a Target Nucleic Acid in a Sample
CA2897474A1 (en) 2013-01-24 2014-07-31 California Institute Of Technology Chromophore-based characterization and detection methods
EP3030680B1 (en) 2013-08-09 2018-10-03 Luminex Corporation Melt discrimination and multiplexing in nucleic acid assays

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69207580T2 (de) * 1992-03-23 1996-08-08 Hoffmann La Roche Verfahren zum DNS-Nachweis
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
US7160996B1 (en) * 1999-06-09 2007-01-09 Bioresearch Technologies, Inc. Fluorescence energy transfer probes with stabilized conformations
US20020197611A1 (en) * 2001-06-21 2002-12-26 Chagovetz Alexander Michael Method for real-time detection and quantification of nucleic acid sequences using fluorescent primers
DE60213730T2 (de) * 2001-08-31 2007-08-16 The University Of Utah Research Foundation, Salt Lake City Echtzeit-Quantifizierung mit internen Standards
US7273730B2 (en) * 2002-05-24 2007-09-25 Invitrogen Corporation Nested PCR employing degradable primers
EP1509624A2 (en) * 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010539940A (ja) * 2007-09-28 2010-12-24 スリーエム イノベイティブ プロパティズ カンパニー 核酸検出の二重オリゴヌクレオチド法

Also Published As

Publication number Publication date
EP1780291A1 (en) 2007-05-02
US20070117125A1 (en) 2007-05-24
RU2005132940A (ru) 2007-11-27
US8198026B2 (en) 2012-06-12

Similar Documents

Publication Publication Date Title
CN107849603B (zh) 利用有限核苷酸组成的引物扩增
ES2494922T3 (es) Métodos para la amplificación, cuantificación e identificación de ácidos nucleicos
JP2007537746A (ja) 2つの異なるアニーリング温度を示すpcr時におけるアンプリコン混入の検出方法
US20110281266A1 (en) Identification of Nucleic Acids
JP7175326B2 (ja) 標的核酸増幅方法及び標的核酸増幅用組成物
KR102295290B1 (ko) Dna 증폭 기술
KR20140091944A (ko) 내부컨트롤 및 리포터 및 소광자가 결합된 pna 프로브를 이용한 용융곡선 분석방법, 이를 이용한 표적핵산 검출방법 및 표적핵산 검출 키트
US20210381067A1 (en) Nucleic acid amplification and detection with attenuaiting probe
US20220170086A1 (en) Looped primer and loop-de-loop method for detecting target nucleic acid
KR102648647B1 (ko) 짧은 호모폴리머릭 반복서열의 개선된 검출법
JP2017521056A (ja) 鎖侵入に基づくdna増幅法
WO2017143873A1 (zh) 一种等温核酸扩增的方法
KR20120018734A (ko) 헤파티티스 b 바이러스 검출용 키트 및 이를 이용한 헤파티티스 b 바이러스의 검출 방법
CN116732147A (zh) 一种用于等温扩增的高特异性引物及其应用
JP2007125011A (ja) ポリメラーゼ連鎖反応の助けによりdna又はrnaの特定のフラグメントを検出する方法
JP2007125011A5 (enExample)
CN108642147A (zh) 一种快速扩增核酸的方法
EP3158082B1 (en) Restriction mediated quantitative polymerase chain reactions
JP2023552546A (ja) マラリアを検出するための組成物および方法
KR102900467B1 (ko) 토홀드 매개 가닥 치환 기반의 rpa-crispr 핵산 검출용 조성물 및 검출 방법
JP6999645B2 (ja) 核酸の増幅及び検出/定量の効率を改良するためのヘルパーオリゴヌクレオチド
CN104988235A (zh) 一种切克酶介导等温扩增小分子信标定量检测技术
CN105506082B (zh) 利用链转移型引物扩增核酸及融合探针的方法
KR101606530B1 (ko) Hla-b*27 및 hla-b*51의 동시 검출 방법 및 이의 용도
US20170356058A1 (en) Compositions and methods for detection of hepatitis c virus genotype 3

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20070619