JP2007006872A - Method for producing fermented drink containing dietary fiber - Google Patents

Method for producing fermented drink containing dietary fiber Download PDF

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JP2007006872A
JP2007006872A JP2005217983A JP2005217983A JP2007006872A JP 2007006872 A JP2007006872 A JP 2007006872A JP 2005217983 A JP2005217983 A JP 2005217983A JP 2005217983 A JP2005217983 A JP 2005217983A JP 2007006872 A JP2007006872 A JP 2007006872A
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dietary fiber
fermentation
water
soluble dietary
raw material
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JP4435721B2 (en
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Takaaki Izumi
貴章 泉
Susumu Furukubo
進 古久保
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Suntory Ltd
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Suntory Ltd
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for producing fermented drinks abundantly containing water soluble dietary fibers and having high acceptability, especially beer taste fermented drinks. <P>SOLUTION: The fermented drink is produced by adding the water soluble dietary fiber to a process after the fermentation process and reducing the rate of assimilable sugar in the dietary fiber to prevent retardation of fermentation caused by the water soluble dietary fiber and solve influence on taste, concretely, dissolve increase of sweet taste. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

本発明は、発酵飲料の製造方法に関し、詳しくは難消化性デキストリン等の食物繊維を含有する発酵飲料の製造方法に関する。  The present invention relates to a method for producing a fermented beverage, and more particularly to a method for producing a fermented beverage containing dietary fibers such as indigestible dextrin.

近年、消費者のニーズの多様化に伴い、酒類においても、健康機能を持たせた製品が検討されている。発酵飲料、なかでも、ビール、発泡酒などビールテイストにおいても、各種機能を持たせた製品が望まれている。
一方、食物繊維は摂取量の不足が問題とされる成分の一つである。食物繊維は水溶性食物繊維と不溶性食物繊維に大別される。このうち、水溶性食物繊維としては、食後の血糖値上昇を抑制する成分として、難消化性デキストリンが知られている。
食物繊維を含有させた酒類として、これまでも検討がなされている。例えば、特許文献1には、ゲンチオオリゴ糖またはその還元処理物を糖質原料の一つとして用いる酒類の製造方法が開示されている。
特許文献2には、ブラックベリー酒に難消化性デキストリンを添加した酒類が開示されている。
特許文献3には、副原料として難消化性デキストリン又はその加水分解物を仕込工程または発酵工程で添加することを特徴とするコク味のある酒類の製造方法が開示されている。
特許文献3には、酵母資化性水溶性食物繊維を副原料として使用する酒類の製造方法が開示されている。
しかしながら、これらの文献には、水溶性食物繊維を高含量で含む飲料について、詳細な製造条件、特に、水溶性食物繊維の添加時期、水溶性食物繊維を含有する原料の組成などの検討は十分には行われていなかった。
In recent years, with the diversification of consumer needs, alcoholic beverages are being considered for products with health functions. In fermented beverages, especially beer tastes such as beer and sparkling liquor, products having various functions are desired.
Dietary fiber, on the other hand, is one of the components where lack of intake is a problem. Dietary fiber is roughly classified into water-soluble dietary fiber and insoluble dietary fiber. Among these, as a water-soluble dietary fiber, an indigestible dextrin is known as a component that suppresses an increase in blood glucose level after a meal.
Studies have been made for alcoholic beverages containing dietary fiber. For example, Patent Document 1 discloses a method for producing an alcoholic beverage using gentiooligosaccharide or a reduced product thereof as one of saccharide raw materials.
Patent Document 2 discloses alcoholic beverages obtained by adding indigestible dextrin to blackberry sake.
Patent Document 3 discloses a method for producing a rich liquor characterized by adding an indigestible dextrin or a hydrolyzate thereof as an auxiliary material in a charging step or a fermentation step.
Patent Document 3 discloses a method for producing alcoholic beverages using yeast-assimilable water-soluble dietary fiber as an auxiliary material.
However, these documents do not fully examine the detailed production conditions, especially the timing of addition of water-soluble dietary fiber and the composition of raw materials containing water-soluble dietary fiber, for beverages containing a high content of water-soluble dietary fiber. Was not done.

特開平4−200378号公報  Japanese Patent Laid-Open No. 4-200378 特開平6−32802号公報  JP-A-6-32802 特許第3609372号明細書  Japanese Patent No. 3609372

本発明は水溶性食物繊維を豊富に含み、且つ嗜好性の高い発酵飲料、特にビールテイスト発酵飲料の製造方法を提供することを目的とする。  An object of this invention is to provide the manufacturing method of fermented drinks which contain water-soluble dietary fiber abundantly, and have high palatability | flavor, especially beer taste fermented drinks.

難消化性デキストリンなどの水溶性食物繊維を含む発酵飲料を製造するにあたり、本発明者らは、水溶性食物繊維を含む副原料について、添加量や各工程に与える影響について鋭意検討した。その結果、一定以上の濃度で水溶性食物繊維を含有させる場合、発酵工程で酵母の増殖抑制に起因する発酵遅延が起きることが判った。その傾向は、元々発酵工程が不安定な傾向にある、麦芽使用比率の低い発酵飲料、特に、麦芽を使用しない発酵飲料で顕著であった。
そこで、本発明者らは、上記発酵工程での不具合を解消するために発酵工程より後の工程で添加すること着想するとともに、添加する食物繊維中の資化性糖比率を低減させることで、発酵工程より後に添加することによることにより、発酵の遅延を防止すると共に、中味への影響、具体的に甘味の増加を解消させ、本発明を完成させた。
In producing fermented beverages containing water-soluble dietary fibers such as indigestible dextrins, the present inventors diligently studied the effects on the added amount and each step of the auxiliary materials containing water-soluble dietary fibers. As a result, it was found that when water-soluble dietary fiber was contained at a certain concentration or more, fermentation delay caused by yeast growth inhibition occurred in the fermentation process. This tendency was conspicuous in fermented beverages having a low malt use ratio, in particular, fermented beverages not using malt, in which the fermentation process tends to be unstable.
Therefore, the present inventors have conceived that it is added in a step after the fermentation step in order to eliminate the inconvenience in the fermentation step, and by reducing the assimilable sugar ratio in the dietary fiber to be added, By adding after the fermentation step, the delay of fermentation was prevented and the influence on the contents, specifically the increase in sweetness, was eliminated, and the present invention was completed.

本発明により、香味の優れた発酵飲料とその製造方法が提供される。特に、麦芽使用比率の低いビールテイスト発酵飲料において、資化性糖由来の甘味がなく、且つ、水溶性食物繊維を高濃度で含有する、香味の優れた発酵飲料とその製造方法が提供される。  The present invention provides a fermented beverage with excellent flavor and a method for producing the same. In particular, in a beer-taste fermented beverage with a low malt use ratio, there is provided a fermented beverage having an excellent flavor, which has no sweetness derived from assimilable sugar and contains a high concentration of water-soluble dietary fiber, and a method for producing the same. .

本発明の使用原料、製造工程、目的の発酵飲料等について更に詳しく説明する。
(水溶性食物繊維)
本発明における水溶性食物繊維とは、水に溶解し、且つ、酵母に資化されないまたは資化されにくい性質をもつ食物繊維をいう。例えば、難消化性デキストリン、ポリデキストロース、ガラクトマンナン、水溶性トウモロコシ繊維などが挙げられる。これらは市販品として購入することができる。市販品の水溶性食物繊維の場合、主成分の含量は、各種含量のグレードのものがある。主成分以外の成分としては、資化性糖や主成分の分解物などが含まれる。
本発明においては、水溶性食物繊維を発酵工程の終了後に添加することから、資化性糖が多いと製品の甘味が増すことになる。従って、本発明においては、水溶性食物繊維を含む副原料として、資化性糖の割合が、20%以下、好ましくは10%以下の水溶性食物繊維を好適に用いることができる。すなわち、水溶性食物繊維を含む副原料として、水溶性食物繊維の含量が固形分換算重量で80%以上、好ましくは90%以上の原料を用いることが好ましい。
(難消化性デキストリン)
本発明において、水溶性食物繊維として、難消化性デキストリンを好適に用いるることができる。難消化性デキストリンは、加熱処理したデンプンをアミラーゼで加水分解し、未分解物より難消化性成分を分取して脱塩、脱色して得ることが出来る。市販の難消化性デキストリンとして、パインファイバーC(松谷化学工業)などがある。当該物質の生理作用として、整腸作用や血糖値上昇抑制作用などが動物実験で認められている〔伊藤、月間フードケミカル、9、78〜83、(1990)〕。本発明は水溶性、難消化性デキストリンであれば使用できるが、これら市販の製品の使用が便利である。
本発明において、水溶性食物繊維を含む副原料として、難消化性デキストリンの含量が固形分換算重量で80%以上、好ましくは90%以上の原料を用いることが好ましい。前に述べたように、資化性糖が多いと製品の甘味が増すことになるからである。
難消化性デキストリンなど水溶性食物繊維を含む副原料の添加量は、最終製品に求める健康感やコク味の設計に基づいて、適宜設定することができる。特に、糖シロップなどに由来する酵母資化性糖と組み合わせて、合計添加量が固形分換算重量で20%以上〜の場合、発酵液中に存在すると発酵遅延を及ぼすことから、発酵工程より後に添加する本発明の技術が有効である。
また、水溶性食物繊維として、難消化性デキストリンの加水分解物を用いてもよい。この場合、当該加水分解物は、酵母に資化されないまたは資化されにくい性質を保持している必要がある。
(添加時期)
本発明の発酵飲料の製造において、難消化性デキストリンなど水溶性食物繊維を含む副原料を添加するが、発酵工程が終了した後に添加することが肝要である。
仕込工程や発酵工程で難消化性デキストリンなど水溶性食物繊維を含む副原料を添加する場合、発酵原液中の水溶性食物繊維の存在によって、発酵工程における酵母の液中の流動性が損なわれる。それにより、不均一な発酵となり、発酵不良を招いてしまう。また発酵不良に付随して、発酵由来の不快臭等が発生してしまう。酵母代謝産物等のオフフレーバーの増大といった発酵不良による欠点が生じる。
従って、水溶性食物繊維を含む副原料の添加は、発酵工程の終了後であって、充填工程の前までに行うことが好ましい。すなわち、貯酒工程(熟成工程ともいう)、調合工程、ろ過工程での添加が好ましい。また、発酵工程であっても、酵母の増殖やアルコール発酵に影響を及ぼさない程度に遅い時機(例えば、発酵期間の三分の二以降)に添加することもできる。中でも、原料由来の異物の除去や、微生物の繁殖防止の観点から、ろ過工程よりも前の、貯酒工程または調合工程での添加が特に好ましい。
本発明において、上述の製造工程をビールテイスト飲料の製造を用いて説明する。
仕込工程とは、例えばビールにおいては麦芽、大麦等原料を必要に応じ糖化、タンパク分解等を施し、ホップ等の副原料を適量添加して煮沸を行い、最終的に酵母よるアルコール発酵に供する麦汁を製造する工程である。
発酵工程とは通常の発酵飲料の製造における発酵工程を指す。すなわち、麦汁等の発酵原液に酵母を添加し、酵母によっての増殖およびアルコール発酵を行わせ、また未熟臭等成分を分解および炭酸ガスによる揮散反応も行わせる工程である。
貯酒工程は完全に発酵終了した発酵液を0℃近い低温で長期間寝かせることで混濁の原因となるタンパク質を析出させ、味を整える工程である。貯酒工程では酵母による生物的代謝反応は全く行われず、発酵工程とはみなされない。
調合工程では、貯酒工程が終了した発酵液に、必要により、甘味料、酸味料、苦味料等の調味料、希釈水、アルコール、pH調製剤などの安定化剤、香料などを添加して飲料の香味や品質を整える。ビール以外の発酵飲料において好適に用いられる工程である。調合工程での酵母による発酵は全く行われない。
濾過工程は発酵終了したもろみから、残存した酵母や不溶成分を完全に取り除いて清澄化し、最終製品とする工程である。
充填工程は濾過工程の終了した製品を缶・ビン・樽等に充填する工程である。
(発酵飲料)
本発明の発酵飲料は酵母による発酵工程を経て製造される飲料を全て包含する。本発明が提供する発酵飲料、そのなかでもビールテイスト発酵飲料とは、炭素源、窒素源、ホップ類などを原料とし、酵母で発酵させた飲料であって、ビールのような風味を有するものをいう。通常は麦芽および大麦、米、とうもろこしなどの穀物類を糖化して得た糖液や、糖類そのものから得た糖液などに、酵母を添加し発酵させる工程を経るが、窒素源としては、麦芽以外の植物由来のタンパク質もしくはその加水分解物を利用する場合もある。ビールテイスト発酵飲料としては、例えば、ビール、発泡酒、雑酒、リキュール類、スピリッツ類、低アルコール発酵飲料(例えばアルコール分1%未満の麦芽発酵飲料)などが挙げられる。
中でも、本発明の技術は、水溶性食物繊維の添加による発酵不良を招きやすい飲料の製造時に特に有用である。すなわち、発酵工程が不安定になりやすい発酵飲料の製造に有用であり、このような発酵飲料としては、1)麦芽使用比率の低い飲料、2)麦芽を使用しない発酵飲料、3)低糖質または低カロリー発酵飲料などが挙げられる。これらの発酵飲料を本発明の製造方法を使用して製造する場合について説明し、併せてそれぞれの発酵飲料を製造する場合に適した炭素源および窒素源の原料等についても説明する。
1)低麦芽使用比率の発酵飲料
麦芽使用比率の低い飲料として、例えば、水を除く原料中の麦芽の使用比率が25%未満の発酵飲料を挙げることができる。これらの飲料においては、麦芽由来の酵素、窒素源、ミネラル分の不足による発酵不良に留意する必要があり、水溶性食物繊維の添加によって、発酵不良になる傾向がある。
2)麦芽を使用しない発酵飲料
麦芽を使用しない発酵飲料としては、例えば、麦芽以外の麦、米、コーンなどの穀物類を原料とする発酵飲料が上げられる。穀物類としては、コーンは、タンパクの構成アミノ酸中に、ロイシンを非常に豊富に含むことがわかっている。ロイシンは発酵飲料、特にビールテイスト飲料の良好な香味の前駆体であることから、コーンを好適に用いることができる。コーンは麦芽同様に発芽させて用いようとしても、発酵工程での発酵不良の問題を伴うが、本発明にしたがい、成分を分画してから用いると発酵不良の問題が解消される。コーンは発芽コーン、未発芽コーンのいずれも本発明に使用できるが、未発芽のコーンの使用が好適である。
3) 低糖質または低カロリー発酵飲料
本発明の発酵飲料の好ましい態様の一つは、低糖質または低カロリー発酵飲料である。低糖質または低カロリー発酵飲料の製造においては、発酵工程で必要な炭素源や窒素源の余剰を少なくして製造することが多く、発酵不良となる傾向がある。更に、低カロリーまたは低糖質を実現するために、発酵液を水で希釈する場合も多い。その場合、発酵液中の水溶性食物繊維の濃度は高くなること。これらの理由で発酵不良となる傾向が強いことから、本発明に技術を好適に用いることができる。
低糖質または低カロリーのビールテイスト飲料は、香り付与が課題であることが多い。とうもろこしタンパク質画分を発酵原料の窒素源として用いるため、十分な発酵により良好な発酵香を与えるものであることから、低糖質または低カロリーのビールテイスト飲料の製造に際し、原料として分画したコーンを好適に用いることができる。特に、酢酸イソアミルが発酵物上清中に、2.0ppmを超え且つ10.0ppm未満まで含まれるように条件を設定して発酵を行うと、得られた発酵飲料を5〜8倍に希釈しても、香味の優れた発酵飲料となることが判明した(特願2005−157921)。希釈は水により行うが、このとき低糖質発酵飲料に不足する呈味物質を補うため、酸味料、甘味料、苦味料、アルコールを一緒に添加してもよく、あるいは呈味物質の補足は、希釈操作後に別途行うこともできる。
糖質の低下は希釈による方法の代わりに、または希釈による方法と組み合わせて、炭素源として酵母が資化しやすい3糖類、2糖類および単糖類の比率を高めた(例えば全炭素源の80%以上にした)、発酵原料を用いることで行うことも可能である。
低糖質とは、発酵飲料中の糖質濃度が、固形分換算で0.8重量%、特に0.5重量%未満であることを意味する。低糖質であることが好ましい発酵飲料には、清酒、ワイン、ビール、発泡酒、リキュール類、スピリッツ類、雑酒、ビールテイスト発酵飲料などが含まれる。特に発泡酒、ビールテイスト発酵飲料が好ましく、特にビールテイスト発酵飲料が好ましい。
また、本発明の別の好適な飲料の例として、低カロリー飲料、特にビールテイストの低カロリー発酵飲料が挙げられる。低カロリー飲料とは、12kcal/100ml未満の飲料であり、低糖質および/または低アルコールとすることによって実現することができる。
以下に、本発明で使用する発酵原料、特に上述の麦芽使用比率の低い発酵飲料、麦芽を使用しない発酵飲料、低糖質または低カロリー発酵飲料製造に適した原料について説明する。
(非麦穀物類としてのコーン)
本発明で使用する穀物類はコーンが好ましいことは前に説明した通りである。コーンは発芽コーン、未発芽コーンのいずれも本発明に使用できるが、未発芽のコーンの使用が好適である。 コーンの種類はデントコーン、フリントコーン、ポップコーン、ワキシーコーン等特に限定されない。このうち、デントコーンがビールテイスト飲料の原料として適している。コーンは、粒状コーンを粉砕して用いてもよいし、デンプンやペプチドが未分画であれば、コーングリッツやコーンフラワーといった加工品を出発原料として用いても良い。
コーンなどの非麦穀物を原料として用いる場合、デンプン成分、タンパク質成分等各成分に分画して用いることができる。
(非麦穀物の成分の分画)
本発明においては麦以外の各種穀物、例えばコーンを各成分に分画してから発酵原料とする。分画により、少なくともデンプン画分およびタンパク画分に分画する。分画方法に特別の制限はないが、例えば、以下の工程で行うことができる。
コーンを浸漬水に浸漬する(浸漬温度はほぼ室温、浸漬時間は48時間程度である);吸水したコーンを粉砕する(好ましい粉砕条件としては、ディスクミルである);粉砕後、水面に浮かんだ胚芽を分離する;胚芽を分離した粉砕コーンを磨砕する(好ましい磨砕条件はディスクミルである);磨砕後のコーンを篩に通し、篩上に残った繊維画分を分離する(好ましい篩は50〜100μm程度である)、遠心分離などで比重の違いに基づいてデンプン画分とタンパク画分とを分離回収する。あるいはこれを、遠心分離で分離しても良い。
(分画成分の酵素分解)
デンプン画分の糖化は、醸造用糖化液の調製のための公知の方法で行うことができる。例えば、外部酵素として液化酵素(アミラーゼ)を用い、スターチ分解物(コーン糖化スターチ)を調製することができる。液化酵素の種類、使用量、酵素反応温度、酵素反応時間は、製造すべき発酵飲料に応じて適宜決定できるが、ビールテイスト飲料の製造を目的とする場合で例示すると例えば、以下のとおりである。
pHを水酸化カルシウムを使いpH6に調整し、耐熱αアミラーゼ(ターマミル120L:ノボ社)を適量(0.025〜0.5%)添加し、よく混合し攪拌しながら103℃で5分間加熱を行う。温度を95℃に下げ、90分間保持する。温度を65℃に下げβアミラーゼ・プルラナーゼ酵素として、ビオザイムM5(アマノエンザイム社)やライスターゼPL(大和化成)を0.01〜0.1%添加して、約20時間攪拌する。それを80℃以上まで昇温を行い酵素類を失活させる。この糖液を珪藻土で濾過し、イオン交換樹脂を通して脱塩を行い、活性炭で脱色・脱臭し、デンプン分解物(コーン糖化スターチ)液を得ることができる。分解が酵母発酵に適する程度に達成されたか否かは資化性糖をHPLCで測定することにより調べることができる。
得られたデンプン分解物(コーン糖化スターチと称することもある)の溶液は、そのままでまたは適宜濃縮もしくは希釈して用いることもできるが、微生物的に安定な程度、例えば固形分75%まで減圧濃縮することが好ましい。
タンパク画分の分解は、発酵段階で酵母が利用するアミノ酸やペプチドといったタンパク分解物を得るために行う。分解のために使用可能な酵素および酵素反応方法は一般に公知であり、例えば以下の方法で分解することができる。
タンパク画分を固形分で30%になるように温水に懸濁し、殺菌のため95℃に昇温し20分間保持する。温度を50℃に下げ、水酸化ナトリウムと塩酸で中性になるようpHを調整し、フレーバーザイム(ノボ社)やプロチンFA(大和化成)といったプロテアーゼを0.1〜1.0%程度添加し20時間反応させる。分解度は遊離アミノ酸が20%以上になるのを目処とし、酵素失活のため95℃に昇温し20分間保持する。この反応液を、珪藻土で濾過し、活性炭で脱色・脱臭を行いコーン蛋白分解液を得る。酵母発酵に適するまで分解されたか否かはHPLCにより調べることができる。
得られたコーン蛋白分解液は、そのままでまたは適宜濃縮もしくは希釈して用いることもできるが、固形分30%まで減圧濃縮し、105℃にて5〜10秒間スプレードライしてコーン蛋白分解物の粉末としても良い。
(ホップ)
ホップについては本発明の発酵飲料が、ビールテイスト発酵飲料である場合の製造に使用する。ホップはビール等の製造に使用する通常のペレットホップ、粉末ホップ、ホップエキスを香味に応じて適宜選択使用する。さらに、イソ化ホップ、ヘキサホップ、テトラホップなどのホップ加工品を用いることもできる。
(酵母)
本発明で用いる酵母は、製造すべき発酵飲料の種類、目的とする香味や発酵条件などを考慮して自由に選択できる。例えばWeihenstephan−34株など、市販のビール酵母を用いることができる。
(酵母増殖発酵助剤)
一方、本発明において、原料として、例えば、分画したコーンを用いた場合など、酵母発酵が充分に進まない場合、酵母増殖発酵助剤を用いることができる。酵母増殖発酵助剤は、一般に知られているもの、例えば、酵母エキス、米や麦などの糠成分、ビタミン、ミネラル剤などを単独または組み合わせて適量使用すればよい。中でも酵母エキスが好適に用いられる。酵母エキスは窒素源として作用する。その使用量は、酵母が旺盛に発酵する範囲であれば特に限定されないが、香味上、とうもろこしタンパク分解物と酵母エキスの重量比が、4:6〜8:2の範囲であることが望ましい。発酵前の原料に添加しても良く、または発酵中に添加してもよい。
(その他の原料)
本発明においては、必要に応じて、色素や泡形成剤、香料、などを添加することができる。色素についてはビール様の色を与えるために使用するものであり、カラメル色素などをビール様の色彩を呈する量添加する。ビール様の泡を形成させるため、大豆サポニン、キラヤサポニン等の植物抽出サポニン系物質、牛血清アルブミン等のタンパク質系物質などを適宜使用する。ビール様の風味付けのためにビール風味を有する香料を適量使用することができる。
The use raw material of the present invention, the production process, the target fermented beverage and the like will be described in more detail.
(Water-soluble dietary fiber)
The water-soluble dietary fiber in the present invention refers to a dietary fiber that dissolves in water and has a property that is not assimilated or hardly assimilated by yeast. For example, indigestible dextrin, polydextrose, galactomannan, water-soluble corn fiber and the like can be mentioned. These can be purchased as commercial products. In the case of commercially available water-soluble dietary fiber, the content of the main component is of various grades. Components other than the main component include assimilable sugars and degradation products of the main component.
In the present invention, since the water-soluble dietary fiber is added after the fermentation process, the sweetness of the product increases when there is a large amount of assimilating sugar. Therefore, in the present invention, water-soluble dietary fiber having a assimilable sugar ratio of 20% or less, preferably 10% or less can be suitably used as an auxiliary material containing water-soluble dietary fiber. That is, as an auxiliary material containing water-soluble dietary fiber, it is preferable to use a material having a water-soluble dietary fiber content of 80% or more, preferably 90% or more in terms of solid content.
(Indigestible dextrin)
In the present invention, an indigestible dextrin can be suitably used as the water-soluble dietary fiber. The indigestible dextrin can be obtained by hydrolyzing the heat-treated starch with amylase, separating the indigestible component from the undegraded product, and desalting and decolorizing. Examples of commercially available indigestible dextrin include Pine Fiber C (Matsuya Chemical Industries). As a physiological action of the substance, an intestinal regulating action, a blood sugar level increase inhibiting action and the like have been recognized in animal experiments [Ito, Monthly Food Chemical, 9, 78-83, (1990)]. Although the present invention can be used as long as it is water-soluble and hardly digestible dextrin, use of these commercially available products is convenient.
In the present invention, as an auxiliary material containing water-soluble dietary fiber, it is preferable to use a material having an indigestible dextrin content of 80% or more, preferably 90% or more in terms of solid content. This is because, as mentioned above, if there is a large amount of assimilating sugar, the sweetness of the product will increase.
The addition amount of the auxiliary raw material containing water-soluble dietary fiber such as indigestible dextrin can be appropriately set based on the health feeling and the rich taste design required for the final product. In particular, in combination with yeast assimilating sugars derived from sugar syrup and the like, when the total addition amount is 20% or more in terms of solid content, since it causes fermentation delay when present in the fermentation broth, after the fermentation step The technique of the present invention to be added is effective.
Moreover, you may use the hydrolyzate of indigestible dextrin as water-soluble dietary fiber. In this case, the hydrolyzate needs to have a property that is not assimilated or hardly assimilated by yeast.
(Additional time)
In the production of the fermented beverage of the present invention, an auxiliary material containing water-soluble dietary fiber such as indigestible dextrin is added, but it is important to add it after the fermentation process is completed.
In the case where an auxiliary material containing water-soluble dietary fiber such as indigestible dextrin is added in the preparation process or fermentation process, the fluidity of the yeast in the fermentation process is impaired due to the presence of the water-soluble dietary fiber in the fermentation stock solution. Thereby, it becomes non-uniform fermentation and invites poor fermentation. In addition, an unpleasant odor or the like derived from fermentation is generated along with poor fermentation. There are disadvantages due to poor fermentation such as an increase in off-flavor such as yeast metabolites.
Therefore, it is preferable to add the auxiliary raw material containing the water-soluble dietary fiber after the end of the fermentation process and before the filling process. That is, the addition in the storage process (also referred to as aging process), the preparation process, and the filtration process is preferable. Moreover, even if it is a fermentation process, it can also be added to the time late | slow (for example after 2/3 of a fermentation period) to such an extent that it does not affect the proliferation of yeast or alcohol fermentation. Among these, from the viewpoint of removing foreign substances derived from raw materials and preventing the growth of microorganisms, the addition in the alcohol storage process or the preparation process prior to the filtration process is particularly preferable.
In the present invention, the above-described production process will be described using production of a beer-taste beverage.
For example, in beer, malt, barley and other raw materials are subjected to saccharification, proteolysis, etc. if necessary, and an appropriate amount of auxiliary materials such as hops are added and boiled, and finally subjected to alcoholic fermentation by yeast. This is a process for producing soup.
A fermentation process refers to the fermentation process in manufacture of a normal fermented drink. That is, it is a step in which yeast is added to a fermentation stock solution such as wort to cause growth by yeast and alcohol fermentation, and to decompose components such as immature odor and volatilization reaction with carbon dioxide gas.
The liquor storage process is a process of adjusting the taste by precipitating proteins that cause turbidity by laying the fermented liquid completely fermented at a low temperature close to 0 ° C. for a long period of time. In the liquor storage process, no biological metabolic reaction is carried out by the yeast and it is not considered a fermentation process.
In the blending process, beverages are added to the fermented liquor after the liquor storage process, if necessary, by adding seasonings such as sweeteners, acidulants and bitters, stabilizers such as dilution water, alcohol and pH adjusters, and flavorings. Of flavor and quality. It is a process suitably used in fermented beverages other than beer. Fermentation with yeast in the blending process is not performed at all.
The filtration process is a process in which the remaining yeast and insoluble components are completely removed from the mash that has been fermented to clarify the final product.
The filling step is a step of filling cans, bottles, barrels, etc. with the product after the filtration step.
(Fermented beverage)
The fermented beverage of the present invention includes all beverages produced through a fermentation process using yeast. Fermented beverages provided by the present invention, among them, beer-taste fermented beverages are beverages fermented with yeast using carbon sources, nitrogen sources, hops, etc., and having a beer-like flavor. Say. Usually, yeast is added to the sugar solution obtained by saccharifying malt and grains such as barley, rice and corn, or the sugar solution obtained from the sugar itself, and fermented. In some cases, other plant-derived proteins or hydrolysates thereof may be used. Examples of the beer-taste fermented beverage include beer, sparkling wine, miscellaneous sake, liqueurs, spirits, and low alcohol fermented beverages (for example, malt fermented beverages having an alcohol content of less than 1%).
Especially, the technique of this invention is especially useful at the time of manufacture of the drink which tends to cause the fermentation failure by addition of water-soluble dietary fiber. That is, it is useful for the production of fermented beverages in which the fermentation process is likely to be unstable. As such fermented beverages, 1) beverages with a low malt use ratio, 2) fermented beverages that do not use malt, 3) low sugars or Examples include low calorie fermented beverages. The case where these fermented drinks are manufactured using the manufacturing method of this invention is demonstrated, and the raw material of the carbon source and nitrogen source etc. which are suitable when manufacturing each fermented drink are also demonstrated.
1) Fermented beverage with a low malt use ratio As a beverage with a low malt use ratio , for example, a fermented beverage in which the use ratio of malt in the raw material excluding water is less than 25% can be mentioned. In these beverages, it is necessary to pay attention to poor fermentation due to lack of malt-derived enzymes, nitrogen sources and minerals, and the addition of water-soluble dietary fibers tends to cause poor fermentation.
2) Fermented beverages that do not use malt Examples of fermented beverages that do not use malt include fermented beverages made from grains such as wheat, rice, and corn other than malt. As cereals, corn has been found to be very rich in leucine in the amino acids that make up proteins. Since leucine is a good flavor precursor for fermented beverages, particularly beer-taste beverages, corn can be suitably used. Even if corn is germinated in the same manner as malt, it is accompanied by a problem of poor fermentation in the fermentation process. However, according to the present invention, the problem of poor fermentation is solved when the components are used after fractionation. As the corn, either a germinated corn or an ungerminated corn can be used in the present invention, but it is preferable to use an ungerminated corn.
3) Low sugar or low calorie fermented beverage One of the preferred embodiments of the fermented beverage of the present invention is a low sugar or low calorie fermented beverage. In the production of a low-sugar or low-calorie fermented beverage, it is often produced by reducing the surplus of a carbon source and a nitrogen source that are necessary in the fermentation process, which tends to cause poor fermentation. Furthermore, the fermentation broth is often diluted with water to achieve low calories or low sugars. In that case, the concentration of water-soluble dietary fiber in the fermentation broth should be high. For these reasons, since the tendency to be poor fermentation is strong, the technique can be suitably used in the present invention.
Low sugar or low calorie beer-taste beverages often have a problem of imparting aroma. Since the corn protein fraction is used as a nitrogen source of the fermentation raw material, it provides a good fermented aroma by sufficient fermentation. Therefore, in the production of a low-sugar or low-calorie beer-taste beverage, It can be used suitably. In particular, when fermentation is performed by setting the conditions so that isoamyl acetate is contained in the supernatant of the fermented product in excess of 2.0 ppm and less than 10.0 ppm, the obtained fermented beverage is diluted 5 to 8 times. However, it became clear that it became a fermented drink with excellent flavor (Japanese Patent Application No. 2005-157721). Dilution is carried out with water. At this time, in order to supplement the taste substances that are lacking in low-sugar fermented beverages, acidulants, sweeteners, bitters, and alcohol may be added together, or supplementation of taste substances It can also be performed separately after the dilution operation.
Carbohydrate reduction increased the ratio of trisaccharides, disaccharides and monosaccharides that are easily assimilated by yeast as a carbon source instead of the dilution method or in combination with the dilution method (for example, 80% or more of the total carbon source) It is also possible to use a fermentation raw material.
The low sugar means that the sugar concentration in the fermented beverage is 0.8% by weight, particularly less than 0.5% by weight, in terms of solid content. Fermented beverages preferably having a low sugar content include sake, wine, beer, sparkling wine, liqueurs, spirits, miscellaneous sake, beer-taste fermented beverages and the like. Happoshu and beer-taste fermented beverages are particularly preferable, and beer-taste fermented beverages are particularly preferable.
In addition, examples of another suitable beverage of the present invention include a low calorie beverage, particularly a beer-taste low calorie fermented beverage. A low-calorie beverage is a beverage of less than 12 kcal / 100 ml, and can be realized by using a low sugar and / or low alcohol.
Below, the fermented raw material used by this invention, especially the fermented drink with the low malt use ratio mentioned above, the fermented drink which does not use malt, the raw material suitable for low-sugar or low-calorie fermented drink manufacture is demonstrated.
(Corn as a non-wheat grain)
As described above, the cereals used in the present invention are preferably corn. As the corn, either a germinated corn or an ungerminated corn can be used in the present invention, but it is preferable to use an ungerminated corn. The type of corn is not particularly limited, such as dent corn, flint corn, pop corn, and waxy corn. Among these, dent corn is suitable as a raw material for beer-taste beverages. The corn may be used by pulverizing granular corn, or if the starch or peptide is unfractionated, a processed product such as corn grits or corn flour may be used as a starting material.
When non-wheat grains such as corn are used as a raw material, they can be fractionated into each component such as starch component and protein component.
(Fractionation of non-wheat grains)
In the present invention, various grains other than wheat, such as corn, are fractionated into respective components, and then used as a fermentation raw material. By fractionation, it is fractionated into at least a starch fraction and a protein fraction. Although there is no special restriction | limiting in the fractionation method, For example, it can carry out at the following processes.
Immerse the corn in immersion water (immersion temperature is about room temperature, immersion time is about 48 hours); pulverize the water-absorbed corn (preferred pulverization condition is a disk mill); Separating the germ; grinding the ground corn from which the germ has been separated (preferred grinding condition is a disc mill); passing the ground corn through a sieve and separating the fiber fraction remaining on the sieve (preferably The sieve fraction is about 50-100 μm), and the starch fraction and the protein fraction are separated and recovered based on the difference in specific gravity by centrifugation or the like. Alternatively, this may be separated by centrifugation.
(Enzymatic degradation of fraction components)
The saccharification of the starch fraction can be performed by a known method for preparing a saccharified solution for brewing. For example, by using a liquefied enzyme (amylase) as an external enzyme, a starch degradation product (corn saccharification starch) can be prepared. The type, amount used, enzyme reaction temperature, and enzyme reaction time of the liquefied enzyme can be appropriately determined according to the fermented beverage to be produced. For example, when the purpose is to produce a beer-taste beverage, it is as follows. .
Adjust the pH to pH 6 using calcium hydroxide, add an appropriate amount (0.025 to 0.5%) of heat-resistant α-amylase (Termamyl 120L: Novo), mix well and heat at 103 ° C for 5 minutes with stirring. Do. The temperature is lowered to 95 ° C. and held for 90 minutes. The temperature is lowered to 65 ° C., and 0.01-0.1% of biozyme M5 (Amanoenzyme) or lyasease PL (Daiwa Kasei) is added as β-amylase / pullulanase enzyme, and stirred for about 20 hours. The temperature is raised to 80 ° C. or higher to deactivate the enzymes. This sugar solution is filtered through diatomaceous earth, desalted through an ion exchange resin, decolorized and deodorized with activated carbon, and a starch decomposition product (corn saccharification starch) solution can be obtained. Whether or not the degradation has been achieved to an extent suitable for yeast fermentation can be examined by measuring the assimilating sugar by HPLC.
The obtained starch degradation product (sometimes referred to as corn saccharified starch) can be used as it is or after appropriately concentrating or diluting, but it is concentrated under reduced pressure to a microbially stable level, for example, 75% solid content. It is preferable to do.
Degradation of the protein fraction is performed in order to obtain proteolytic products such as amino acids and peptides used by yeast in the fermentation stage. Enzymes and enzyme reaction methods that can be used for the decomposition are generally known, and can be decomposed, for example, by the following method.
Suspend the protein fraction in warm water to a solid content of 30%, raise the temperature to 95 ° C. for sterilization and hold for 20 minutes. Lower the temperature to 50 ° C, adjust the pH to neutral with sodium hydroxide and hydrochloric acid, and add about 0.1-1.0% of protease such as Flavorzyme (Novo) or Protin FA (Daiwa Kasei). React for 20 hours. The degree of degradation is set so that the free amino acid is 20% or more, and the temperature is raised to 95 ° C. and kept for 20 minutes for enzyme deactivation. The reaction solution is filtered through diatomaceous earth, and decolorized and deodorized with activated carbon to obtain a corn protein solution. It can be checked by HPLC whether or not it has been degraded to suit yeast fermentation.
The obtained corn proteolysate can be used as it is or after appropriately concentrating or diluting, but it is concentrated under reduced pressure to a solid content of 30% and spray-dried at 105 ° C. for 5 to 10 seconds. It may be a powder.
(hop)
About hop, it uses for manufacture in case the fermented drink of this invention is a beer taste fermented drink. As for hops, normal pellet hops, powder hops, and hop extracts used for the production of beer and the like are appropriately selected and used according to the flavor. Furthermore, hop processed products such as isopized hop, hexahop, and tetrahop can be used.
(yeast)
The yeast used in the present invention can be freely selected in consideration of the type of fermented beverage to be produced, the intended flavor and fermentation conditions. For example, commercially available brewer's yeast, such as Weihenstephan-34 strain, can be used.
(Yeast growth fermentation aid)
On the other hand, in the present invention, when the yeast fermentation does not proceed sufficiently, for example, when a fractionated corn is used as a raw material, a yeast growth fermentation aid can be used. The yeast growth fermentation aid may be used in an appropriate amount of generally known ones such as yeast extract, rice bran ingredients such as rice and wheat, vitamins and mineral agents. Of these, yeast extract is preferably used. Yeast extract acts as a nitrogen source. The amount used is not particularly limited as long as the yeast is vigorously fermented, but it is desirable that the weight ratio of the corn proteolysate and the yeast extract is in the range of 4: 6 to 8: 2. You may add to the raw material before fermentation, or you may add during fermentation.
(Other raw materials)
In the present invention, pigments, foam-forming agents, fragrances, and the like can be added as necessary. The pigment is used to give a beer-like color, and caramel pigment or the like is added in an amount exhibiting a beer-like color. In order to form beer-like bubbles, plant-extracted saponin substances such as soybean saponin and quilla saponin, and protein substances such as bovine serum albumin are appropriately used. An appropriate amount of perfume having a beer flavor can be used for beer-like flavoring.

本発明を実施例により更に詳しく説明する。ただし、これらの説明は本発明の範囲を限定するものではない。  The present invention will be described in more detail with reference to examples. However, these explanations do not limit the scope of the present invention.

麦芽を使用しないビールテイスト飲料について、水溶性食物繊維の添加が酵母発酵に与える影響を検討した。
仕込工程で表1に示す組成の発酵原液を調製した。対象品1−1、1−2については、炭素源として四糖類以上の糖組成が7%の糖シロップ(加藤化学製)、窒素源としてコーンタンパク分解物、発酵促進剤として酵母エキス、着色料としてカラメルを添加し、難消化性デキストリン含量の異なる2種類の水溶性食物繊維分含有副原料(食物繊維AまたはB)を添加した後、仕込水を加水して総量6kgとした。一方、水溶性食物繊維分含有副原料を調合工程で添加することとした対象品1−3および発明品1ついては、水溶性食物繊維分含有副原料を除いて、同様に発酵原液を調製した。
食物繊維Aは市販品(松谷化学製)の難消化性デキストリン含量が90%のものを用いた。食物繊維Bは食物繊維Aにマルトースを適量配合し、難消化性デキストリン含量が75%のものを調製して用いた。
得られた4種類の調製液を60分間煮沸し、その後30分間静置してホップ粕を除き、発酵原液を得た(表1を参照)。
次に発酵工程で、これら発酵原液に、ビール酵母(Weihenstephan−34株)を生菌数20×10cells/mlになるように添加し、温度13℃にて8日間発酵させた。
次に貯酒工程にて、発酵終了した液を0℃にて3日間静置した。
更に、調合工程にて、甘味料、酸味料、苦味料、脱気水を適量加え、更に対象品1−3および発明品1については、難消化性デキストリン含量の異なる2種類の水溶性食物繊維分含有副原料(食物繊維AまたはB)を合わせて添加し、最終的に約5倍容量に希釈した。
更に、濾過工程でフィルターろ過を行ない、酵母および不溶成分を取り除いた。
最後に、充填工程にて、瓶詰を行ない、4種類のアルコール度約2%のビールテイスト発酵飲料を得た。
発酵工程の良悪の指標として、発酵4日目の資化性糖の残量が5%以上の場合を不良(×)、5%未満の場合を良好(○)とした(表2)。その結果、対象品1−1と対象品1−2では酵母の増殖不良による発酵遅延が認められた。これは発酵液中の粘性の上昇による酵母の流動性の低下に起因すると考えられた。
また、得られた製品について、未熟成臭としてジアセチルおよび甘味を評価した。
評価は官能試験により行った。訓練された6人のパネラーにより、ジアセチルまたは甘味の有無を、強く感じる(3点)、感じる(2点)、やや感じる(1点)、感じない(0点)の4段階で評価し、平均点が、0〜1点を○、1〜2点を△、2〜3点を×とした(表3)。
更に、製品のジアセチル含量を定法(EBC法)に準じてガスクロマトグラフィで評価した(表3)。
For beer-taste beverages that do not use malt, the effect of adding water-soluble dietary fiber on yeast fermentation was examined.
A fermentation stock solution having the composition shown in Table 1 was prepared in the preparation step. Regarding the target products 1-1 and 1-2, a sugar syrup having a sugar composition of 7% or more as a carbon source (made by Kato Chemical Co.) as a carbon source, a corn proteolysate as a nitrogen source, a yeast extract as a fermentation accelerator, and a coloring agent Caramel was added, and two types of water-soluble dietary fiber-containing auxiliary materials (dietary fiber A or B) having different indigestible dextrin contents were added, and then the feed water was added to make a total amount of 6 kg. On the other hand, for the target product 1-3 and the inventive product 1 in which the water-soluble dietary fiber-containing auxiliary material was added in the preparation step, a fermentation stock solution was similarly prepared except for the water-soluble dietary fiber-containing auxiliary material.
As the dietary fiber A, a commercially available product (manufactured by Matsutani Chemical Co., Ltd.) having an indigestible dextrin content of 90% was used. Dietary fiber B was prepared by adding an appropriate amount of maltose to dietary fiber A and having a less digestible dextrin content of 75%.
The obtained four kinds of prepared solutions were boiled for 60 minutes, and then allowed to stand for 30 minutes to remove hop koji and obtain a fermentation stock solution (see Table 1).
Next, in the fermentation process, brewer's yeast (Weihenstephan-34 strain) was added to these fermentation stock solutions so as to have a viable cell count of 20 × 10 6 cells / ml and fermented at a temperature of 13 ° C. for 8 days.
Next, in the liquor storage step, the liquid after fermentation was allowed to stand at 0 ° C. for 3 days.
Furthermore, in the preparation process, appropriate amounts of sweetener, sour agent, bitter agent and deaerated water are added, and for the target product 1-3 and the inventive product 1, two types of water-soluble dietary fibers having different indigestible dextrin contents are added. Minor-containing secondary ingredients (dietary fiber A or B) were added together and finally diluted to about 5 times volume.
Further, filtration was performed in the filtration step to remove yeast and insoluble components.
Finally, the bottle was bottled in the filling step to obtain four types of beer-taste fermented beverages having an alcohol content of about 2%.
As an indicator of the quality of the fermentation process, a case where the remaining amount of the assimilating sugar on the fourth day of fermentation was 5% or more was judged as bad (x) and a case where it was less than 5% was judged as good (◯) (Table 2). As a result, in the target product 1-1 and the target product 1-2, fermentation delay due to poor growth of yeast was observed. This was thought to be due to a decrease in yeast fluidity due to an increase in viscosity in the fermentation broth.
Moreover, about the obtained product, diacetyl and sweetness were evaluated as an immature odor.
Evaluation was performed by a sensory test. Six trained panelists evaluated the presence or absence of diacetyl or sweetness in four levels: feel strongly (3 points), feel (2 points), feel slightly (1 point), do not feel (0 points), and average In the points, 0 to 1 points were indicated as ◯, 1-2 points as Δ, and 2-3 points as X (Table 3).
Furthermore, the diacetyl content of the product was evaluated by gas chromatography according to a standard method (EBC method) (Table 3).

Figure 2007006872
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以上より、純度の高い水溶性食物繊維を、発酵終了に添加することで、発酵工程の不良を生じることなく、甘味の少ない、且つコクのある発酵飲料が得られることが判った。    From the above, it was found that a fermented beverage with less sweetness and richness can be obtained without adding a poor fermentation process by adding high-purity water-soluble dietary fiber at the end of fermentation.

水溶性食物繊維の添加量について検討した。
仕込工程で表4に示す組成の発酵原液を調製し、実施例1と同様にビールテイスト発酵飲料を調製した。
水溶性食物繊維として、食物繊維A(市販品、難消化性デキストリン含量が90%のもの)を用いた。これを対象品(2−2、2−3、2−4)については、仕込工程で、発明品(2−1,2−2,2−3)については、調合濾過工程で添加した。対象品2−1では、食物繊維Aを添加しなかった。発酵工程および貯酒工程は実施例1に準じた。調合工程では、甘味料、酸味料、苦味料、脱気水、および食物繊維A(発明品2−1,2−2,2−3)に加え、醸造用アルコールを添加して、アルコール度を5%とし、酵母および不溶成分を取り除き製品を得た。ろ過工程および充填工程は実施例1に準じた。
得られた発酵飲料について、甘味、ジアセチル臭、およびコク味を実施例1と同様の方法で官能評価した。すなわち、訓練された6人のパネラーにより、甘味、ジアセチル臭、およびコク味の有無を、強く感じる(3点)、感じる(2点)、やや感じる(1点)、感じない(0点)の4段階で評価し、平均点が、0〜1点を○、1〜2点を△、2〜3点を×とした(表5)。
その結果、水溶性食物繊維の添加量に比して、コク味が付与された。しかしながら、一定量以上の水溶性食物繊維を仕込工程で添加した対象品2−3,2−4では未熟成臭(ジセセチル臭)が認められた。
一方、水溶性食物繊維を調合工程で添加した本発明品はいずれも良好な香味の発酵飲料であった。
The amount of water-soluble dietary fiber added was examined.
A fermentation stock solution having the composition shown in Table 4 was prepared in the preparation step, and a beer-taste fermented beverage was prepared in the same manner as in Example 1.
As water-soluble dietary fiber, dietary fiber A (commercially available product with a content of indigestible dextrin of 90%) was used. This was added in the preparation step for the target product (2-2, 2-3, 2-4) and in the preparation filtration step for the inventive product (2-1, 2-2, 2-3). In the target product 2-1, dietary fiber A was not added. The fermentation process and the liquor storage process were in accordance with Example 1. In the blending process, in addition to the sweetener, sour agent, bitter agent, deaerated water, and dietary fiber A (Invention products 2-1, 2-2, 2-3), alcohol for brewing is added, and the alcohol level is adjusted. The product was obtained by removing 5% of yeast and insoluble components. The filtration step and the filling step were in accordance with Example 1.
About the obtained fermented drink, sensory evaluation was carried out by the method similar to Example 1 about sweet taste, diacetyl smell, and richness. That is, six trained panelists strongly feel (3 points), feel (2 points), feel slightly (1 point), and do not feel (0 points) the presence or absence of sweetness, diacetyl odor, and richness. Evaluation was made in four stages, and the average score was 0 for 1 point, Δ for 1 to 2 points, and × for 2 to 3 points (Table 5).
As a result, a rich taste was imparted compared to the amount of water-soluble dietary fiber added. However, an immature odor (discetyl odor) was observed in the target products 2-3 and 2-4 to which a certain amount or more of water-soluble dietary fiber was added in the preparation process.
On the other hand, the products of the present invention to which water-soluble dietary fiber was added in the preparation process were all fermented beverages with good flavor.

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以上より、水溶性食物繊維を、発酵終了に添加することで、発酵工程の不良を生じることなく、甘味の少ない、且つコクのある発酵飲料が得られることが判った。  From the above, it was found that by adding water-soluble dietary fiber at the end of fermentation, a fermented beverage with less sweetness and richness can be obtained without causing a poor fermentation process.

水溶性食物繊維を添加する工程の違いが製品に与える影響について検討した。
表6に示す組成で仕込液を調製した。水溶性食物繊維として、食物繊維A(市販品、難消化性デキストリン含量が90%のもの)を用いた。
水溶性食物繊維を仕込工程で添加する場合(対照品3−1)、煮沸前の仕込液を1L回収し、食物繊維Aを溶解させたのちタンクに戻した。
水溶性食物繊維を発酵工程で添加する場合(対照品3−2)、発酵原液に酵母を添加した後、1L回収し、食物繊維Aを溶解させたのちタンクに戻した。
水溶性食物繊維を貯酒工程で添加する場合(発明品3−1)、発酵終了後に0℃とした発酵液を1L回収し、食物繊維Aを溶解させたのちタンクに戻した。
水溶性食物繊維を調合工程で添加する場合(発明品3−2)、希釈用の脱気水の一部(1L)を回収し、食物繊維Aを溶解させたのち脱気水に戻した。
水溶性食物繊維をろ過工程で添加する場合(発明品3−3)、ろ過直前の液を1L回収し、食物繊維Aを溶解させた後、ろ過前の液に戻した。
それ以外は、実施例1と同様の工程を経て、アルコール度約2%の5種類のビールテイスト発酵飲料を得た。
得られた発酵飲料について、甘味、ジアセチル臭、更にはコク味を実施例1と同様の方法で官能評価した。すなわち、訓練された6人のパネラーにより、甘味、ジアセチル臭、およびコク味の有無を、強く感じる(3点)、感じる(2点)、やや感じる(1点)、感じない(0点)の4段階で評価し、平均点が、0〜1点を○、1〜2点を△、2〜3点を×とした(表7)。
The effect of the process of adding water-soluble dietary fiber on the product was examined.
A feed solution was prepared with the composition shown in Table 6. As water-soluble dietary fiber, dietary fiber A (commercially available product with a content of indigestible dextrin of 90%) was used.
When water-soluble dietary fiber was added in the charging step (control product 3-1), 1 L of the charging solution before boiling was collected, and after dietary fiber A was dissolved, it was returned to the tank.
When water-soluble dietary fiber was added in the fermentation process (control product 3-2), 1 L was recovered after yeast was added to the fermentation stock solution, and dietary fiber A was dissolved and returned to the tank.
When water-soluble dietary fiber was added in the liquor storage process (Invention 3-1), 1 L of the fermented liquid at 0 ° C. was collected after the fermentation was completed, and dietary fiber A was dissolved and returned to the tank.
When water-soluble dietary fiber was added in the preparation step (Invention product 3-2), a portion (1 L) of deaerated water for dilution was recovered, and after dietary fiber A was dissolved, it was returned to deaerated water.
When water-soluble dietary fiber was added in the filtration step (Invention product 3-3), 1 L of the liquid just before the filtration was recovered, and after the dietary fiber A was dissolved, it was returned to the liquid before filtration.
Other than that, 5 kinds of beer-taste fermented beverages having an alcohol content of about 2% were obtained through the same steps as in Example 1.
About the obtained fermented drink, sensory evaluation was carried out by the method similar to Example 1 about sweet taste, diacetyl smell, and also kokumi. That is, six trained panelists strongly feel (3 points), feel (2 points), feel slightly (1 point), and do not feel (0 points) the presence or absence of sweetness, diacetyl odor, and richness. Evaluation was made in four stages, and the average score was 0 for 1 point, Δ for 1 to 2 points, and × for 2 to 3 points (Table 7).

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その結果、いずれの飲料とも、甘味は少なく、コク味は付与されていた。未熟成臭(ジアセチル臭)は水溶性食物繊維を仕込工程または発酵工程で添加した飲料で認められ、発酵終了後、すなわち貯酒工程、調合工程またはろ過工程で添加した飲料では、認めれらなかった。  As a result, all beverages had a low sweetness and a rich taste. An immature odor (diacetyl odor) was observed in beverages in which water-soluble dietary fiber was added in the preparation step or fermentation step, and was not observed in beverages added after the end of fermentation, that is, in the alcohol storage step, preparation step or filtration step.

食物繊維の種類や、発酵飲料の種類(ビール、発泡酒)を変えた製造例を追加したい。仕込工程として粉砕麦芽1.5kgを、10kgの仕込水と懸濁し、糖化槽内で攪拌温度調節にて糖化およびタンパク分解を行った後、麦汁濾過にて加水しながらできるだけエキス分を回収した。そこに市販糖シロップ(加藤化学)を12kg添加し、仕込水加水後、最終的にエキス分10重量%の麦汁100Lに調整した。これにホップを60g添加して60分間煮沸した後、静置にてホップ粕等を除去して最終麦汁を得た。
次に発酵工程として、麦汁に0.5%の市販ビール酵母(Weihenstephan−34株)を添加し、13℃で約一週間発酵させた。
次に貯酒工程として発酵終了もろみを0℃で3日間保持した。
更に調合工程にて難消化性デキストリン(松谷化学製)を最終5重量%濃度になるように添加し、引き続きろ過工程にて、フィルターろ過を行ない、酵母および不溶成分を取り除いた。最後に、充填工程にて、瓶詰を行い、アルコール度約5%の発泡酒を作成した。
本実施例での発酵工程での発酵は非常に良好で、得られた発泡酒は、未熟臭等のオフフレーバーが一切感じられず、甘味も目立たない、しかも深いコク味を有した高嗜好度のビールテイスト発酵飲料であった。
I want to add a production example that changes the type of dietary fiber and the type of fermented beverage (beer, sparkling liquor). As a preparation process, 1.5 kg of pulverized malt was suspended with 10 kg of charge water, and after saccharification and proteolysis by adjusting the stirring temperature in a saccharification tank, the extract was collected as much as possible while adding water through wort filtration. . 12 kg of commercially available sugar syrup (Kato Kagaku) was added thereto, and after adding water to the feed water, it was finally adjusted to 100 L of wort having an extract content of 10% by weight. After adding 60 g of hops and boiling for 60 minutes, the hop koji and the like were removed by standing to obtain the final wort.
Next, as a fermentation process, 0.5% commercial beer yeast (Weihenstephan-34 strain) was added to wort and fermented at 13 ° C. for about one week.
Next, the fermentation end moromi was kept at 0 ° C. for 3 days as a storage process.
Furthermore, indigestible dextrin (manufactured by Matsutani Chemical Co., Ltd.) was added to a final concentration of 5% by weight in the preparation step, and subsequently, filtration was performed in the filtration step to remove yeast and insoluble components. Finally, the bottle was bottled in the filling step to produce a sparkling liquor having an alcohol content of about 5%.
Fermentation in the fermentation process in this example is very good, and the resulting sparkling liquor does not feel any off-flavor such as unripe odor, has an inconspicuous sweetness, and has a deep taste. It was a beer-taste fermented beverage.

本発明によれば、香味の優れた発酵飲料、特に、麦芽使用比率の低いビールテイスト発酵飲料において、資化性糖由来の甘味がなく、且つ、水溶性食物繊維を高濃度で含有する、香味の優れた発酵飲料を製造することができる。  According to the present invention, a fermented beverage having an excellent flavor, particularly a beer-taste fermented beverage with a low malt use ratio, has no sweetness derived from an assimilating sugar, and contains a high concentration of water-soluble dietary fiber. Excellent fermented beverages can be produced.

Claims (22)

発酵飲料を製造するにあたり、水溶性食物繊維を含有する副原料を、発酵工程の後で添加することを特徴とする発酵飲料の製造方法。  In producing a fermented beverage, a method for producing a fermented beverage, comprising adding a secondary material containing water-soluble dietary fiber after the fermentation step. 水溶性食物繊維を含有する副原料を、発酵工程の後で、充填工程よりも前で添加することを特徴とする請求項1に記載の方法。  The method according to claim 1, wherein the auxiliary material containing water-soluble dietary fiber is added after the fermentation step and before the filling step. 水溶性食物繊維を含有する副原料を、熟成工程、調合工程またはろ過工程において添加することを特徴とする請求項1または2に記載の方法。  The method according to claim 1 or 2, wherein the auxiliary material containing water-soluble dietary fiber is added in the aging step, the blending step, or the filtration step. 水溶性食物繊維を含有する副原料を、熟成工程または調合工程において添加することを特徴とする請求項1、2または3に記載の方法。  The method according to claim 1, 2 or 3, wherein an auxiliary raw material containing water-soluble dietary fiber is added in the aging step or the blending step. 水溶性食物繊維が難消化性デキストリンまたはその分解物であることを特徴とする請求項1〜4の何れかの項に記載の方法。  The method according to any one of claims 1 to 4, wherein the water-soluble dietary fiber is indigestible dextrin or a degradation product thereof. 水溶性食物繊維が難消化性デキストリンであることを特徴とする請求項5に記載の方法。  The method according to claim 5, wherein the water-soluble dietary fiber is indigestible dextrin. 水溶性食物繊維を含有する副原料が、資化性糖を20重量%以下の量で含む原料であることを特徴とする請求項1〜6の何れかの項に記載の方法。  The method according to any one of claims 1 to 6, wherein the auxiliary raw material containing water-soluble dietary fiber is a raw material containing an assimilating sugar in an amount of 20% by weight or less. 水溶性食物繊維を含有する副原料が、資化性糖を10重量%以下の量で含む原料であることを特徴とする請求項7に記載の方法。  The method according to claim 7, wherein the auxiliary raw material containing water-soluble dietary fiber is a raw material containing 10% by weight or less of assimilable sugar. 水を除く原料中の水溶性食物繊維の使用比率が10〜80重量%であることを特徴とする請求項1〜8の何れかの項に記載の方法。  The method according to any one of claims 1 to 8, wherein the use ratio of water-soluble dietary fiber in the raw material excluding water is 10 to 80% by weight. 発酵飲料がビールテイスト発酵飲料であることを特徴とする請求項1〜9の何れかの項に記載の方法。  The method according to claim 1, wherein the fermented beverage is a beer-taste fermented beverage. 発酵飲料が低糖質ビールテイスト飲料または低カロリービールテイスト飲料であることを特徴とする請求項10に記載の方法。  The method according to claim 10, wherein the fermented beverage is a low-sugar beer-taste beverage or a low-calorie beer-taste beverage. 熟成工程後に、発酵液を水で希釈する工程を含むことを特徴とする請求項1〜11の何れかの項に記載の方法。  The method according to any one of claims 1 to 11, further comprising a step of diluting the fermentation broth with water after the aging step. 水を除く原料中の麦の使用比率が25重量%未満である発酵飲料であることを特徴とする請求項1〜12の何れかの項に記載の方法。  The method according to any one of claims 1 to 12, which is a fermented beverage in which the use ratio of wheat in the raw material excluding water is less than 25% by weight. 麦を原料中に含まないことを特徴とする請求項13に記載の方法。  The method according to claim 13, wherein wheat is not contained in the raw material. 原料中の炭素源の75重量%以上が糖類として配合され、残りの炭素源が穀物類が糖化されることによって生じる糖類であることを特徴とする請求項1〜14の何れかの項に記載の方法。  The carbon source in the raw material is blended in an amount of 75% by weight or more as a saccharide, and the remaining carbon source is a saccharide produced by saccharification of cereals. the method of. 穀物をデンプン画分とタンパク画分に分画してから、それぞれ、炭素源および窒素源として発酵原料に用いることを特徴とする請求項1〜15の何れかの項に記載の方法。  The method according to any one of claims 1 to 15, wherein the cereal is fractionated into a starch fraction and a protein fraction and then used as a fermentation raw material as a carbon source and a nitrogen source, respectively. デンプン画分を分解してデンプン分解物を得、別途、タンパク画分を分解してタンパク分解物を得、それら分解物を発酵原料として用いることを特徴とする請求項16に記載の方法。  The method according to claim 16, wherein the starch fraction is decomposed to obtain a starch degradation product, and the protein fraction is separately decomposed to obtain a protein degradation product, and these degradation products are used as fermentation raw materials. 穀物がコーンであることを特徴とする請求項1〜17の何れかの項に記載の方法。  The method according to claim 1, wherein the cereal is corn. 原料中に更に増殖発酵促進剤を配合することを特徴とする請求項1〜18の何れかの項に記載の方法。  The method according to any one of claims 1 to 18, wherein a growth fermentation promoter is further blended in the raw material. 増殖発酵促進剤が酵母エキスであることを特徴とする請求項19に記載の方法。  The method according to claim 19, wherein the growth fermentation promoter is a yeast extract. タンパク分解物と酵母エキスとの重量比が、4:6〜8:2である請求項20に記載の方法。  The method according to claim 20, wherein the weight ratio of the proteolysate to the yeast extract is 4: 6 to 8: 2. 請求項1〜21に記載の製造方法で得られる発酵飲料。  A fermented beverage obtained by the production method according to claim 1.
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