JP2011142922A - Method for producing fermented beverage having regulated remaining nitrogen content - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a technique for providing a beverage having stability and flavor in producing a fermented beverage by using cereals as a raw material. <P>SOLUTION: The method for producing the fermented beverage with enhanced deliciousness and preservation stability in producing the beverage using a degradation product of cereal proteins as the raw material, free from malt and barley includes controlling of the amount of the remaining nitrogen source in the fermented beverage to be produced within the range of 1-10 (mg/100 mL). <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a fermented beverage made from non-wheat grains such as corn. In particular, the present invention relates to a beer-taste beverage having excellent stability and good flavor.

  Conventionally, attempts have been frequently made to brew with 100% ungerminated grain raw materials in fermented beverages such as beer-taste beverages. However, when using such fermentation raw materials, there are various problems to be overcome.

  One problem is that it is different from conventional beer and happoshu preparations and does not contain any enzymes (such as amylases or proteases) that are inherent in the malt. However, the saccharified solution produced in this way does not sufficiently react with the external enzyme, and the sugars and amino acids, which are the nutrients of yeast in the fermentation process, are brought to the target amount required for fermentation. In some cases, this may not occur. In order to deal with this problem, the present inventors fractionated a starch fraction and a protein fraction from a cereal raw material, and treated both fractions with an external enzyme to obtain a starch degradation product and a protein degradation product, respectively. And if it uses as a raw material of the preparation liquid of a fermentation process, it discovers that the malfunction in a preparation process will be solved, and has applied for it as Japanese Patent Application No. 2005-80708.

  Another problem was that when non-wheat grains, especially corn, were used after fractionating the starch fraction and the protein fraction, vitamins and minerals were deficient, leading to poor fermentation in the fermentation process. In order to cope with this problem, the present inventors have proposed in Japanese Patent Application No. 2005-88218 to use a yeast extract having specific properties as a fermentation aid.

Yet another problem is that beer-taste beverages brewed with 100% ungerminated cereal raw materials have room for improvement in terms of flavor, and there is room for improvement in stability of quality in terms of storage. That is.
Japanese Patent Application No. 2005-80708 Japanese Patent Application No. 2005-88218

  Therefore, this invention makes it a subject to provide the technique which provides the drink which satisfy | fills characteristics, such as stability and a flavor, when manufacturing fermented drinks using the raw material which fraction-processed grain, such as corn.

  In accordance with the disclosure of Japanese Patent Application No. 2005-80708, the present inventors have produced a fermented beverage using a raw material obtained by fractionating grains such as corn, and the amount of amino nitrogen contained in the fermented beverage produced. I thought that it might give flavor and stability, and examined the effect in detail. As a result, it has been found that if the amount of amino nitrogen in the fermented beverage to be produced is controlled within a certain range, it is possible to increase the quality and preference of the beer-taste beverage product. Furthermore, it has been found that adjustment of the amount of amino nitrogen in a beer-taste beverage can be achieved by controlling the amount of nitrogen source used, such as a cereal-derived protein degradation product in the fermentation process.

  As a result, by using an optimal amount of nitrogen source such as cereal-derived proteolysate for fermented beverages, a technology for providing a high-preference beer-taste beverage that does not impair flavor quality and resistance to deterioration has been completed.

〔The invention's effect〕
According to the present invention, in the production of fermented beverages using raw materials obtained by fractionating grain raw materials, the residual nitrogen content in the fermented beverages is adjusted, and a high-preference beer-taste beverage that does not impair flavor quality and deterioration resistance is provided. be able to. Hereinafter, the present invention will be described by taking a beer-taste beverage (hereinafter sometimes referred to as a beer-taste beverage) as an example of a fermented beverage, but the following description applies to fermented beverages in general unless otherwise specified.

In the case of producing a beer-taste beverage using fractionated processed raw material of amino nitrogen as the main fermentation material and using no or only a small amount of wheat or malt, It has been found that optimal control of the amount of residual amino nitrogen is one of the decisive factors for imparting desirable flavor and storage stability. If the amount of residual amino nitrogen is too small, umami and richness will be insufficient, and if the amount of residual amino nitrogen is too large, the storage stability of the beer-taste beverage will be impaired. Therefore, in the method of the present invention, the amount of amino nitrogen remaining in the beer-taste beverage is within a certain range, particularly between 1 and 10 (mg / 100 ml), more preferably between 3 and 8 (mg / 100 ml). Control in between. The main component of residual amino nitrogen in the beer-taste beverage is an amino acid such as glutamic acid, and the amount thereof can be measured by a known method, for example, a ninhydrin color reaction.

  In order to control the amino nitrogen remaining in the beer-taste beverage within the above range, for example, the amount of amino nitrogen in the fermentation stock solution is set to a predetermined value, for example, 10 to 20 (mg / 100 ml). For that purpose, the amount of the protein fraction fractionated from the grain raw material (for example, corn) is set to about 1 to 10 g / L. However, in the production of the beer-taste beverage of the present invention, wheat and / or malt may be used in the fermentation raw material to less than about 25%, and / or yeast extract is used according to the disclosure of Japanese Patent Application No. 2005-88218. In such a case, the amount of protein fraction derived from the grain raw material may be slightly lower than the above range depending on the amount of wheat, malt and / or yeast extract used.

  Residual amino nitrogen can also be controlled by the amount of yeast assimilated in the fermentation process. In this case, the amount of residual amino nitrogen can be controlled by controlling the degree of yeast growth under fermentation process conditions, for example, temperature.

Examples of cereal grains are corn, wheat such as malt and barley, rice, buckwheat, sorghum, straw, fins, and beans such as soybeans and peas.

  Of these grains, corn has been found to be very rich in leucine in the amino acids that make up the protein. Since leucine is a good flavor precursor for fermented beverages, particularly beer-taste beverages, corn can be suitably used. Even if corn is germinated in the same manner as malt, it is accompanied by problems such as poor fermentation due to poor enzymatic degradation in the preparation process, but in accordance with the present invention, the problem of poor fermentation occurs when the ingredients are fractionated. Is resolved. As the corn, either a germinated corn or an ungerminated corn can be used in the present invention, but it is preferable to use an ungerminated corn.

  The type of corn is not particularly limited, such as dent corn, flint corn, pop corn, and waxy corn. Among these, dent corn is suitable as a raw material for beer-taste beverages. The corn may be used by pulverizing granular corn, or if the starch or peptide is unfractionated, a processed product such as corn grits or corn flour may be used as a starting material.

Fractionation of cereal components In the present invention, cereals such as corn are fractionated into respective components and used as a fermentation raw material. By fractionation, it is fractionated into at least a starch fraction and a protein fraction. Although there is no special restriction | limiting in the fractionation method, For example, it can carry out at the following processes.

  Immerse the corn in immersion water (immersion temperature is about room temperature, immersion time is about 48 hours); pulverize the absorbed corn (preferred pulverization condition is a disc mill); Separating the germ; grinding the ground corn from which the germ has been separated (preferred grinding condition is a disc mill); passing the ground corn through a sieve and separating the fiber fraction remaining on the sieve (preferably The sieve fraction is about 50 to 100 μm), and the starch fraction and the protein fraction are separated and recovered based on the difference in specific gravity by centrifugation or the like. Alternatively, this may be separated by centrifugation.

The saccharification of the enzymatically degraded starch fraction of the fraction component can be performed by a known method for preparing a saccharified solution for brewing. For example, by using a liquefied enzyme (amylase) as an external enzyme, a starch degradation product (corn saccharification starch) can be prepared. The type, amount used, enzyme reaction temperature, and enzyme reaction time of the liquefied enzyme can be appropriately determined according to the fermented beverage to be produced. For example, when the purpose is to produce a beer-taste beverage, it is as follows. .

  Adjust the pH to pH 6 using calcium hydroxide, add an appropriate amount (0.025 to 0.5%) of heat-resistant α-amylase (Termamyl 120L: Novo), mix well and heat at 103 ° C for 5 minutes with stirring. Do. The temperature is lowered to 95 ° C. and held for 90 minutes. The temperature is lowered to 65 ° C., and 0.01-0.1% of biozyme M5 (Amanoenzyme) or lyasease PL (Daiwa Kasei) is added as β-amylase / pullulanase enzyme and stirred for about 20 hours. The temperature is raised to 80 ° C. or higher to deactivate the enzymes. This sugar solution is filtered through diatomaceous earth, desalted through an ion exchange resin, decolorized and deodorized with activated carbon, and a starch decomposition product (corn saccharification starch) solution can be obtained. Whether or not the degradation has been achieved to an extent suitable for yeast fermentation can be examined by measuring the assimilating sugar by HPLC.

  The obtained starch degradation product (sometimes referred to as corn saccharified starch) can be used as it is or after appropriately concentrating or diluting, but it is concentrated under reduced pressure to a microbially stable level, for example, 75% solid content. It is preferable to do.

  Degradation of the protein fraction is performed in order to obtain proteolytic products such as amino acids and peptides used by yeast in the fermentation stage. Enzymes and enzyme reaction methods that can be used for the decomposition are generally known, and can be decomposed, for example, by the following method.

  Suspend the protein fraction in warm water to a solid content of 30%, raise the temperature to 95 ° C. for sterilization and hold for 20 minutes. Lower the temperature to 50 ° C, adjust the pH to neutral with sodium hydroxide and hydrochloric acid, and add about 0.1 to 1.0% of protease such as Flavorzyme (Novo) or Protin FA (Daiwa Kasei). React for 20 hours. The degree of degradation is set so that the free amino acid is 20% or more, and the temperature is raised to 95 ° C. and kept for 20 minutes for enzyme deactivation. The reaction solution was filtered through diatomaceous earth, and decolorized and deodorized with activated carbon to obtain a corn protein solution. It can be checked by HPLC whether or not it has been degraded to suit yeast fermentation.

  The obtained corn proteolysate can be used as it is or after appropriately concentrating or diluting, but it is concentrated under reduced pressure to a solid content of 30% and spray-dried at 105 ° C. for 5 to 10 seconds. It may be a powder.

The high-temperature and high-pressure treated fiber fraction of the fiber fraction can be suitably used as a flavoring ingredient for beer-taste beverages by treating with a high-temperature and high-pressure fluid (for example, saturated steam or water). If the temperature of the fluid at the time of fluid processing is about 170 to 200 degreeC, it will not specifically limit. It is preferable that it is 180 to 200 degreeC among the said temperature range. When the treatment temperature is less than 170 ° C., there is little effect of increasing the components contributing to imparting richness and irritation, and when the treatment temperature is higher than 200 ° C., the amount of vanillin produced increases rapidly, the vanilla flavor becomes stronger, and the flavor Depending on the design, it may not be suitable as a raw material for fermented beverages.

Moreover, you may mix and process other corn origin components, such as a corn grits, in a fiber fraction. The fluid treatment is desirably performed under a low oxygen concentration.
In the raw material of the fermented beverage, the addition amount of the fiber fraction of the corn subjected to the high-temperature and high-pressure treatment is not particularly limited as long as a fermented beverage having a good flavor is obtained, but for example, 0.01 to 1 per raw material excluding water % Can be added.

Fermented beverage The fermented beverage of the present invention includes all beverages produced through a fermentation process using yeast. Examples include sparkling liquor, beer, low alcohol fermented beverages (for example, fermented beverages having an alcohol content of less than 1%), miscellaneous sake, liqueurs and spirits. Among them, the beer-taste beverage refers to a beverage fermented with yeast using a carbon source, a nitrogen source, hops, etc. as a raw material and having a flavor like beer. Examples of the beer-taste beverage include miscellaneous sake, liqueurs, spirits, and low alcohol fermented beverages (for example, malt fermented beverages having an alcohol content of less than 1%).

  The alcohol content of the fermented beverage of the present invention is not particularly limited, but is preferably 1 to 15% (v / v). In particular, it is desirable that the concentration be the same as that of alcohol that is preferably consumed by consumers as a malt fermented beverage such as beer or sparkling liquor, that is, in the range of 3 to 8% (v / v).

As a carbon source and a nitrogen source carbon source, a sugar solution obtained by saccharifying grains such as rice and corn, a sugar solution obtained from saccharide itself, or the like can be used. The addition amount in the fermentation stock solution is preferably about 100 to 150 g / L.

As the nitrogen source, a plant-derived protein such as corn or a hydrolyzate thereof can be used. Commercial protein degradation products may be used.
In the present invention, it is important that the amount of amino nitrogen in the produced beer-taste beverage is 1 to 10 (mg / 100 ml). For example, the amount of amino nitrogen in the beer-taste beverage is adjusted to 1 to 10 (mg / 100 ml) by adjusting the amount of nitrogen source used in the fermentation raw material. For this purpose, although depending on the type and blending amount of other raw materials, when the main nitrogen source is corn proteolysate, the amount added to the fermentation stock solution is preferably about 1 to 10 g / L.

  The present invention can be suitably used for fermented beverages that use little or no wheat (malt and barley). Specifically, when the use ratio of wheat (malt and barley) is 25% or more by weight ratio in the raw material excluding water, the nitrogen source is sufficiently supplied from the wheat, so it is necessary to use the technique of the present invention. Absent.

Therefore, in the present invention, the use ratio of wheat (malt and barley) can be suitably used when it is less than 25% by weight in the raw material excluding water.
In the case of using wheat such as malt, an internal enzyme or an external enzyme may be used and mixed with the fermentation stock solution after a preparation process by a conventional method.

Production of fermented beverages In the present invention, as a raw material for fermented beverages, starch decomposed products and protein decomposed products obtained by fractionating and processing corn, and optionally adding hops and mixing, boiling the mixture, Yeast is added to the clear undiluted fermentation broth after solid-liquid separation to perform yeast fermentation. Solid-liquid separation may be performed after fermentation or only after fermentation.

In more detail, the following ingredients are prepared and mixed for the production of a fermented beverage:
(1) The amount of starch degradation product used can be about 0.1 to 99% per raw material excluding water and hops (if used). The starch degradation product is preferably obtained by enzymatic treatment of corn by the above-described method, but commercially available saccharified starch or starch syrup made from corn may be used.
(2) On the other hand, the amount of the proteolysate used can be 0.1 to 50 g / L per fermentation stock solution. The proteolytic product is preferably obtained by fractionating from corn by the above-mentioned method and decomposing with a foreign enzyme, but a commercially available corn proteolytic product (corn peptide) or the like may be used.

In the case of a preferred embodiment of the present invention in which a raw material having a large amount of enzyme such as malt is not used as a sugar source for fermentation, for example, 95 to 99.9% starch per raw material excluding water and hop (if used) When 1-10 g / L is mix | blended with a decomposition product and fermentation undiluted | stock fermentation, it is preferable in terms of the avoidance of the fermentation failure in a fermentation process, and the outstanding flavor of the fermented drink obtained.
(3) The use of hops is necessary in order to give a beer flavor in the case of producing beer including beer-taste beverages, sparkling liquors, liqueurs, spirits. As for hops, normal pellet hops, powder hops, and hop extracts used for the production of beer and the like are appropriately selected and used according to the flavor. Furthermore, hop processed products such as isopized hop, hexahop, and tetrahop can be used. The amount used is 0.1 to 10 g per liter of sugar solution.
(4) Further, as a desired component, a fraction other than the starch fraction and the protein fraction separated during the fractionation of non-wheat grains can be appropriately processed and used as desired. Among these, the fiber fraction can be suitably used as a flavoring ingredient for beer-taste beverages, for example, by treating with high-temperature and high-pressure steam at about 170 ° C. to 200 ° C. The amount added is preferably about 0.01 to 1% per raw material excluding water and hops (when used).
(5) The fermentation raw material may be supplemented with fermentation aids such as yeast extract and yeast food, other sugar sources, nitrogen sources and the like, if necessary.

  The above components (1) and (2), and if necessary, part or all of (3) and (4), part or all of (5) are mixed, dispersed in water, and about 100 ° C. Sterilize by heating and separate the supernatant if necessary to make a fermentation stock solution.

  The yeast used for the production of the fermented beverage can be freely selected in consideration of the type of fermented beverage to be produced, the intended flavor and fermentation conditions. For example, a commercially available brewer's yeast such as Weihenstephan-34 strain can be used.

  The fermentation time and fermentation temperature in the production of a fermented beverage can be freely selected in consideration of the type of fermented beverage to be produced, the intended flavor and fermentation conditions. For example, in the production of a beer-taste beverage, fermentation is performed until the fermentation temperature is set to 13 ° C. and a predetermined alcohol concentration is obtained.

  The mixture after fermentation may be used as it is as a fermented beverage, or as a fermented beverage by sterilization such as filtration and heating. A pigment | dye, a foam formation agent, a fragrance | flavor, water-soluble dietary fiber, etc. can be added to a fermented drink as needed. The pigment is used to give a beer-like color, and caramel pigment or the like is added in an amount exhibiting a beer-like color. In order to form beer-like bubbles, plant-extracted saponin substances such as soybean saponin and quilla saponin, and protein substances such as bovine serum albumin are appropriately used. An appropriate amount of perfume having a beer flavor can be used for beer-like flavoring. Further, water-soluble dietary fibers such as indigestible dextrin, polydextrose, water-soluble corn fiber and the like can be added as necessary.

Low sugar or low calorie fermented beverage The technology of the present invention can also be applied to a low sugar fermented beverage. Low sugar means that the sugar concentration in the fermented beverage is 0.8% by weight, particularly less than 0.5% by weight, in terms of solid content. Fermented beverages preferably having a low sugar content include sake, wine, beer, sparkling wine, liqueurs, spirits, miscellaneous sake, beer-taste fermented beverages and the like. Happoshu and beer-taste fermented beverages are particularly preferable, and beer-taste fermented beverages are particularly preferable.

  As one method for producing a low sugar fermented beverage, there is a method of diluting a fermented liquid after fermentation with water or the like. At this time, the dilution rate is usually arbitrary, but by diluting so as to be the amount of the residual nitrogen source shown in the present invention, a low-sugar fermented beverage having excellent stability and good flavor can be produced. . Dilution is carried out with water. At this time, in order to supplement the taste substances that are lacking in low-sugar fermented beverages, acidulants, sweeteners, bitters, and alcohol may be added together, or supplementation of taste substances It can also be carried out separately after the dilution operation (Japanese Patent Application 2005-157921).

  Carbohydrate reduction increased the proportion of trisaccharides, disaccharides and monosaccharides that yeast is likely to assimilate as a carbon source instead of or in combination with the dilution method (e.g. 80% or more of the total carbon source) It is also possible to use fermentation raw materials (Japanese Patent Application No. 2005-157921).

  Another example of a suitable beverage is a low-calorie beverage, particularly a beer-taste low-calorie fermented beverage. Since the low calorie beverage is a beverage having a calorie of less than 20 kcal / 100 ml and can be realized by making it low in sugar and / or low alcohol, the technology of the present invention can be used similarly.

The present invention will be further described below with reference to examples, but the present invention is not limited to the examples.
Example 1
The effects of amino nitrogen content in beer-taste beverages on flavor and stability were investigated.

  Various beer-taste fermented beverages were prepared with the raw materials listed in Table 1. All the raw materials used were commercially available. Corn protein degradation products, soybean protein degradation products, and wheat protein degradation products were used as nitrogen sources. Yeast extract was used as a yeast fermentation promoter. Yeast extract was used as a constant amount, and the amount of various protein degradation products was adjusted so that a beverage having a target amino nitrogen content was obtained.

  About these 6 samples, hop boiling, clarification, saccharification liquid cooling, fermentation, and filtration bottled were performed by the usual method. By these operations, six types of beer-taste beverages were obtained. About the obtained drink, the hydrogen sulfide density | concentration, the presence or absence of richness at the time of drinking, and storage stability were evaluated.

  As for the hydrogen sulfide concentration, the concentration in the headspace gas at the end of fermentation was measured by the GC method. The measurement conditions were as follows. Column: packed column (manufactured by Shimadzu Corporation), column temperature: 80-100 ° C., carrier gas: nitrogen, detector: FPD.

  The body was evaluated by the sensory test method for the presence or absence of body during drinking by 10 trained panelists. The temperature of the sample was 5 ° C. For storage stability, the bottled samples were stored at 28 ° C. for 3 weeks, and the presence or absence of deterioration odor (cardboard odor) was evaluated. In addition, amino nitrogen in beer-taste beverages was measured using a ninhydrin colorimetric method. In addition, the same method was used also when measuring the amino nitrogen of fermentation undiluted | stock solution and a preparation liquid.

  The results are shown in Table 2. The beverage (sample 1) outside the present invention with an amino nitrogen content of 0 mg / 100 ml had an odor of odor due to the generation of hydrogen sulfide, which was thought to be caused by nitrogen source depletion, and lacked umami and richness. Further, it was found that the sample (sample 4) having an amino nitrogen content of 20 mg / 100 ml was inferior in storage stability although it had strong umami and richness.

On the other hand, the beer-taste beverages (Sample 2 and Sample 3) of the present invention having an amino nitrogen content of 1 to 10 mg / 100 ml are beverages that have no odor of odor, have umami taste, richness, and good storage stability. It was.
That is, when producing fermented beverages using raw materials obtained by fractionating corn and other grains, the flavor quality is improved by making the amino nitrogen content of beer-taste beverages 1 to 10 mg / 100 ml. It was found that a beer-taste beverage with a high degree of preference that does not impair deterioration resistance can be provided.

Production Example 1
(Invention 1) Non-wheat fermented beverage production example 100 L of a 10% by weight sugar solution was prepared using a commercially available sugar syrup. Add 0.2% corn proteolysate, 0.03% caramel, 0.03% hops, 0.01% corn fiber fraction treated at high temperature and high pressure, then add 0.2% yeast extract as a fermentation aid and boil for 60-90 minutes. The hop koji was removed by standing to obtain a fermentation stock solution.

  0.5% of commercially available brewer's yeast (Weihenstephan-34 strain) was added to this fermentation stock solution and fermented at 10-15 ° C. for about one week. After storing at 0 ° C. for 3 days, the yeast was removed with a filter, and carbon dioxide was added to prepare a beer-taste beverage. The amount of amino nitrogen in this beverage was 5 mg / 100 ml.

(Invention product 2) Example of production of fermented beverage with low malt use ratio 1.5 kg of crushed malt is suspended with 10 kg of charged water, saccharified and proteolyzed by adjusting the stirring temperature in the saccharification tank, and then filtered through wort The extract was recovered as much as possible while adding water. Thereto, 12 kg of Brix75 commercial sugar syrup was added, and after adding water to the feed water, 100 L of 10 wt% sugar solution was finally prepared. To this was added corn proteolysate 0.1%, caramel 0.02%, hops 0.03%, yeast extract 0.1%, boiled for 60-90 minutes, and hop koji was removed by standing to obtain a fermentation stock solution.

  0.5% of commercially available brewer's yeast (Weihenstephan-34 strain) was added to this fermentation stock solution and fermented at 10-15 ° C. for about one week. After storing at 0 ° C. for 3 days, the yeast was removed with a filter, and carbon dioxide was added to prepare a beer-taste beverage. The amount of amino nitrogen in this beverage was 6 mg / 100 ml.

(Invention 3) Example of production of low-sugar, low-calorie fermented beverage 15 kg of sugar syrup (made by Kato Chemical Co., Ltd.) with a sugar composition of 7% or more of tetrasaccharides and 85 g of corn proteolysate with respect to 85 kg of feed water ), Yeast extract 200 g (0.2% by weight), caramel pigment 200 g, pellet hop 160 g, and high-temperature and high-pressure treated (180 ° C. 1.8 MPa for 1 minute) corn fiber fraction was added 10 g. These were boiled for 60 minutes, and then allowed to stand to remove hop koji to obtain a fermentation stock solution. To this fermentation stock solution, brewer's yeast (Weihenstephan-34 strain) was added at 20 × 10 ⁶ cells / ml and fermented at a temperature of 13 ° C. for 8 days. After the carbon source assimilation was completed, the yeast was removed by filtration, diluted 5 times with 15 L of degassed water to 5 L of the post-fermentation liquid, and carbon dioxide was added to prepare a low sugar fermented beverage. The amount of amino nitrogen in this beverage was 1.2 mg / 100 ml. The obtained beverage had a sugar concentration of 0.46 w / w%, calories of 12 kcal / 100 ml, and alcohol of 1.5 v / v%. The amount of carbohydrate was 0.46.

  The high-temperature and high-pressure processed product of the corn proteolysate and corn fiber fraction used in Invention 3 was a patent application filed by the applicant on the same day as the present application (name of invention: fractionated corn was used). Fermented beverages) produced in Example 3 and Example 4.

  Fermentation defects were not observed during the manufacture of inventions 1, 2 and 3. Moreover, the obtained fermented drink had suitable aroma and richness, and had sufficient storage stability. The results are shown in Table 3.

Claims (7)

In the method for producing a fermented beverage using a proteolytic product of cereal and using no malt or barley, the amount of residual nitrogen source in the produced fermented beverage is controlled between 1 and 10 (mg / 100 ml). The manufacturing method characterized by the above-mentioned. The amount of amino nitrogen in the fermented beverage produced is controlled between 1 and 10 (mg / 100 ml) by adjusting the amount of amino nitrogen in the fermentation broth to 10 to 20 (mg / 100 ml). Of producing a fermented beverage. The method for producing a fermented beverage according to claim 1 or 2, wherein the cereal is selected from wheat, rice, buckwheat, sorghum, rice bran, mackerel, beans, or corn. The method for producing a fermented beverage according to claim 3, wherein the cereal is beans. The method for producing a fermented beverage according to claim 3, wherein the cereal is wheat, soybean or corn. The method for producing a fermented beverage according to claim 1 or 2, wherein the cereal is a non-wheat cereal. A method for stabilizing a fermented beverage, wherein the amount of residual nitrogen source in the fermented beverage to be produced is 1 to 10 (mg) in the method for producing a fermented beverage using a protein degradation product of cereal and not using any malt or barley. / 100 ml), wherein the method is controlled.