JP2006501805A - 有機分子を除去するための方法およびデバイス - Google Patents
有機分子を除去するための方法およびデバイス Download PDFInfo
- Publication number
- JP2006501805A JP2006501805A JP2003555174A JP2003555174A JP2006501805A JP 2006501805 A JP2006501805 A JP 2006501805A JP 2003555174 A JP2003555174 A JP 2003555174A JP 2003555174 A JP2003555174 A JP 2003555174A JP 2006501805 A JP2006501805 A JP 2006501805A
- Authority
- JP
- Japan
- Prior art keywords
- biological sample
- sample mixture
- solid phase
- phase extraction
- particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 182
- 239000000463 material Substances 0.000 claims abstract description 133
- 239000000203 mixture Substances 0.000 claims abstract description 95
- 239000012472 biological sample Substances 0.000 claims abstract description 76
- 238000002414 normal-phase solid-phase extraction Methods 0.000 claims abstract description 60
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 43
- 239000011159 matrix material Substances 0.000 claims abstract description 35
- 239000007787 solid Substances 0.000 claims abstract description 33
- 239000002245 particle Substances 0.000 claims description 92
- 238000012545 processing Methods 0.000 claims description 88
- 238000012163 sequencing technique Methods 0.000 claims description 88
- 239000000853 adhesive Substances 0.000 claims description 73
- 230000001070 adhesive effect Effects 0.000 claims description 73
- 230000008569 process Effects 0.000 claims description 56
- 239000011541 reaction mixture Substances 0.000 claims description 55
- 150000007523 nucleic acids Chemical class 0.000 claims description 34
- 108020004707 nucleic acids Proteins 0.000 claims description 31
- 102000039446 nucleic acids Human genes 0.000 claims description 31
- 239000010410 layer Substances 0.000 claims description 22
- 230000003321 amplification Effects 0.000 claims description 18
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 18
- 239000005546 dideoxynucleotide Substances 0.000 claims description 17
- 239000001226 triphosphate Substances 0.000 claims description 17
- 235000011178 triphosphate Nutrition 0.000 claims description 17
- 238000003491 array Methods 0.000 claims description 16
- 238000003860 storage Methods 0.000 claims description 15
- 238000011049 filling Methods 0.000 claims description 14
- 239000013039 cover film Substances 0.000 claims description 12
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims description 9
- 229920001296 polysiloxane Polymers 0.000 claims description 7
- 239000004820 Pressure-sensitive adhesive Substances 0.000 claims description 6
- 229920000620 organic polymer Polymers 0.000 claims description 6
- 239000004593 Epoxy Substances 0.000 claims description 5
- 229920002313 fluoropolymer Polymers 0.000 claims description 5
- 238000002372 labelling Methods 0.000 claims description 5
- 229920003052 natural elastomer Polymers 0.000 claims description 5
- 229920001194 natural rubber Polymers 0.000 claims description 5
- 229920002037 poly(vinyl butyral) polymer Polymers 0.000 claims description 5
- 229920003051 synthetic elastomer Polymers 0.000 claims description 5
- 239000005061 synthetic rubber Substances 0.000 claims description 5
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 claims description 4
- 239000001177 diphosphate Substances 0.000 claims description 4
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims description 4
- 235000011180 diphosphates Nutrition 0.000 claims description 4
- 125000000524 functional group Chemical group 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 239000010954 inorganic particle Substances 0.000 claims description 3
- 150000004712 monophosphates Chemical class 0.000 claims description 3
- 239000002861 polymer material Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 2
- 239000012790 adhesive layer Substances 0.000 claims 2
- 229920006124 polyolefin elastomer Polymers 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 description 72
- 239000000975 dye Substances 0.000 description 70
- 239000000523 sample Substances 0.000 description 66
- 239000013615 primer Substances 0.000 description 48
- 239000000047 product Substances 0.000 description 43
- 238000006243 chemical reaction Methods 0.000 description 42
- 238000000746 purification Methods 0.000 description 41
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 35
- 239000002131 composite material Substances 0.000 description 28
- 239000000243 solution Substances 0.000 description 21
- 108020004414 DNA Proteins 0.000 description 14
- 238000005251 capillar electrophoresis Methods 0.000 description 14
- -1 nucleotide triphosphates Chemical class 0.000 description 13
- 239000007790 solid phase Substances 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 108091093088 Amplicon Proteins 0.000 description 11
- 238000001712 DNA sequencing Methods 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 11
- 238000002156 mixing Methods 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 239000011248 coating agent Substances 0.000 description 9
- 238000000576 coating method Methods 0.000 description 9
- 150000003384 small molecules Chemical class 0.000 description 9
- 238000001962 electrophoresis Methods 0.000 description 8
- 230000008901 benefit Effects 0.000 description 7
- 239000012535 impurity Substances 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- 230000001351 cycling effect Effects 0.000 description 6
- 239000010408 film Substances 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 239000002773 nucleotide Substances 0.000 description 6
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 239000000919 ceramic Substances 0.000 description 5
- 239000007795 chemical reaction product Substances 0.000 description 5
- 238000004891 communication Methods 0.000 description 5
- 238000010828 elution Methods 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 238000012856 packing Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 239000003643 water by type Substances 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 238000013019 agitation Methods 0.000 description 4
- 239000012491 analyte Substances 0.000 description 4
- 238000005352 clarification Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 229920000098 polyolefin Polymers 0.000 description 4
- 239000011148 porous material Substances 0.000 description 4
- 239000002987 primer (paints) Substances 0.000 description 4
- 238000007480 sanger sequencing Methods 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000002390 adhesive tape Substances 0.000 description 3
- 238000009739 binding Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 3
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 3
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 3
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 3
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 3
- 239000007857 degradation product Substances 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 239000002777 nucleoside Substances 0.000 description 3
- 150000003833 nucleoside derivatives Chemical class 0.000 description 3
- 230000005298 paramagnetic effect Effects 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 238000011027 product recovery Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000000376 reactant Substances 0.000 description 3
- 238000004007 reversed phase HPLC Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 229920001400 block copolymer Polymers 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000005081 chemiluminescent agent Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 229920001577 copolymer Polymers 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 238000003618 dip coating Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 239000000017 hydrogel Substances 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000007834 ligase chain reaction Methods 0.000 description 2
- 230000000873 masking effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000003068 molecular probe Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 229920013639 polyalphaolefin Polymers 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 108091033319 polynucleotide Proteins 0.000 description 2
- 102000040430 polynucleotide Human genes 0.000 description 2
- 239000002157 polynucleotide Substances 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 239000012264 purified product Substances 0.000 description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 229920002379 silicone rubber Polymers 0.000 description 2
- 239000004945 silicone rubber Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000010345 tape casting Methods 0.000 description 2
- 229920001897 terpolymer Polymers 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- AVBGNFCMKJOFIN-UHFFFAOYSA-N triethylammonium acetate Chemical compound CC(O)=O.CCN(CC)CC AVBGNFCMKJOFIN-UHFFFAOYSA-N 0.000 description 2
- 238000009827 uniform distribution Methods 0.000 description 2
- 238000003260 vortexing Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241000585703 Adelphia <angiosperm> Species 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- 229910000503 Na-aluminosilicate Inorganic materials 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- ABLZXFCXXLZCGV-UHFFFAOYSA-N Phosphorous acid Chemical class OP(O)=O ABLZXFCXXLZCGV-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004721 Polyphenylene oxide Substances 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 229910052581 Si3N4 Inorganic materials 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 150000001252 acrylic acid derivatives Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 125000003277 amino group Chemical class 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000005518 carboxamido group Chemical class 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000012792 core layer Substances 0.000 description 1
- 238000003851 corona treatment Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000004924 electrostatic deposition Methods 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000003517 fume Substances 0.000 description 1
- 239000005350 fused silica glass Substances 0.000 description 1
- 238000001641 gel filtration chromatography Methods 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000000608 laser ablation Methods 0.000 description 1
- 239000012633 leachable Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000058 polyacrylate Chemical class 0.000 description 1
- 229920001515 polyalkylene glycol Polymers 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229920002631 room-temperature vulcanizate silicone Polymers 0.000 description 1
- 239000012146 running buffer Substances 0.000 description 1
- 238000001878 scanning electron micrograph Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000007650 screen-printing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
- 230000005476 size effect Effects 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 239000000429 sodium aluminium silicate Substances 0.000 description 1
- 235000012217 sodium aluminium silicate Nutrition 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 125000001273 sulfonato group Chemical group [O-]S(*)(=O)=O 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012876 topography Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 150000003673 urethanes Chemical class 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T428/00—Stock material or miscellaneous articles
- Y10T428/25—Web or sheet containing structurally defined element or component and including a second component containing structurally defined particles
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25125—Digestion or removing interfering materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Extraction Or Liquid Replacement (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/027,226 US7347976B2 (en) | 2001-12-20 | 2001-12-20 | Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix |
| PCT/US2002/038143 WO2003054510A2 (en) | 2001-12-20 | 2002-11-26 | Method and device for removal of organic molecules |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006501805A true JP2006501805A (ja) | 2006-01-19 |
| JP2006501805A5 JP2006501805A5 (https=) | 2006-03-02 |
Family
ID=21836445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003555174A Pending JP2006501805A (ja) | 2001-12-20 | 2002-11-26 | 有機分子を除去するための方法およびデバイス |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US7347976B2 (https=) |
| EP (1) | EP1458485A4 (https=) |
| JP (1) | JP2006501805A (https=) |
| AU (1) | AU2002359526A1 (https=) |
| CA (1) | CA2469999A1 (https=) |
| WO (1) | WO2003054510A2 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8164828B2 (en) | 2006-12-26 | 2012-04-24 | Nsk Ltd. | Observable centrifugal apparatus and observation apparatus |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7192560B2 (en) * | 2001-12-20 | 2007-03-20 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
| US7347976B2 (en) * | 2001-12-20 | 2008-03-25 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix |
| US6889468B2 (en) | 2001-12-28 | 2005-05-10 | 3M Innovative Properties Company | Modular systems and methods for using sample processing devices |
| US7198759B2 (en) * | 2002-07-26 | 2007-04-03 | Applera Corporation | Microfluidic devices, methods, and systems |
| US7981600B2 (en) * | 2003-04-17 | 2011-07-19 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene |
| US20140011201A1 (en) * | 2003-05-13 | 2014-01-09 | Ibis Biosciences, Inc. | Method for the purification of targeted nucleic acids from background nucleic acids |
| US7238269B2 (en) * | 2003-07-01 | 2007-07-03 | 3M Innovative Properties Company | Sample processing device with unvented channel |
| US20060275919A1 (en) * | 2003-08-20 | 2006-12-07 | Charlotte Lindhardt | Methods for extraction and concetration of hydrophilic compounds from hydrophobic liquid matrices |
| US20050130177A1 (en) | 2003-12-12 | 2005-06-16 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US7322254B2 (en) * | 2003-12-12 | 2008-01-29 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US7727710B2 (en) * | 2003-12-24 | 2010-06-01 | 3M Innovative Properties Company | Materials, methods, and kits for reducing nonspecific binding of molecules to a surface |
| US7939249B2 (en) | 2003-12-24 | 2011-05-10 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
| US20050142571A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using solid phase material |
| DE102004062281A1 (de) * | 2003-12-29 | 2005-07-28 | Siemens Ag | Verfahren und Spotting-Lösung zum Herstellen von Microarrays |
| US7754474B2 (en) | 2005-07-05 | 2010-07-13 | 3M Innovative Properties Company | Sample processing device compression systems and methods |
| US7323660B2 (en) | 2005-07-05 | 2008-01-29 | 3M Innovative Properties Company | Modular sample processing apparatus kits and modules |
| US7763210B2 (en) | 2005-07-05 | 2010-07-27 | 3M Innovative Properties Company | Compliant microfluidic sample processing disks |
| JP4984849B2 (ja) * | 2006-11-27 | 2012-07-25 | パナソニック株式会社 | 成分分離デバイスと、この成分分離デバイスを用いた化学分析デバイス |
| US20090042241A1 (en) | 2007-04-06 | 2009-02-12 | California Institute Of Technology | Microfluidic device |
| US20100129878A1 (en) * | 2007-04-25 | 2010-05-27 | Parthasarathy Ranjani V | Methods for nucleic acid amplification |
| US20090269746A1 (en) * | 2008-04-25 | 2009-10-29 | Gil Atzmon | Microsequencer-whole genome sequencer |
| USD638550S1 (en) | 2009-11-13 | 2011-05-24 | 3M Innovative Properties Company | Sample processing disk cover |
| USD638951S1 (en) | 2009-11-13 | 2011-05-31 | 3M Innovative Properties Company | Sample processing disk cover |
| USD667561S1 (en) | 2009-11-13 | 2012-09-18 | 3M Innovative Properties Company | Sample processing disk cover |
| US8834792B2 (en) | 2009-11-13 | 2014-09-16 | 3M Innovative Properties Company | Systems for processing sample processing devices |
| CN103648649A (zh) | 2011-05-18 | 2014-03-19 | 3M创新有限公司 | 检测选定体积的材料在样本处理装置中存在的系统和方法 |
| MX336625B (es) | 2011-05-18 | 2016-01-26 | 3M Innovative Properties Co | Sistemas y metodos para medicion volumetrica en dispositivo de procesamiento de muestra. |
| US9067205B2 (en) | 2011-05-18 | 2015-06-30 | 3M Innovative Properties Company | Systems and methods for valving on a sample processing device |
| USD672467S1 (en) | 2011-05-18 | 2012-12-11 | 3M Innovative Properties Company | Rotatable sample processing disk |
| CA2883672C (en) * | 2012-08-07 | 2021-03-30 | Environmental Express, Inc. | Hydrophilic activated sorbent extraction disk |
| US20150307927A1 (en) * | 2012-12-15 | 2015-10-29 | Tangen Biosciences, Inc. | Apparatus for centrifuge mountable manifold for processing fluidic assays |
| US10610861B2 (en) | 2012-12-17 | 2020-04-07 | Accellix Ltd. | Systems, compositions and methods for detecting a biological condition |
| EP2932268A4 (en) | 2012-12-17 | 2016-10-19 | Leukodx Ltd | SYSTEMS AND METHOD FOR RECOGNIZING A BIOLOGICAL CONDITION |
| US20140170678A1 (en) | 2012-12-17 | 2014-06-19 | Leukodx Ltd. | Kits, compositions and methods for detecting a biological condition |
| WO2015112914A1 (en) | 2014-01-24 | 2015-07-30 | Life Technologies Corporation | Purification chemistries and formats for sanger dna sequencing reactions on a micro-fluidics device |
| US11559801B2 (en) | 2014-11-03 | 2023-01-24 | Tangen Biosciences, Inc. | Apparatus and method for cell, spore, or virus capture and disruption |
| WO2017172702A1 (en) | 2016-03-28 | 2017-10-05 | Tangen Biosciences, Inc. | Apparatus and method for extracting pathogens from biological samples |
| CN110390871A (zh) * | 2016-08-15 | 2019-10-29 | 南通立方新材料科技有限公司 | 一种热固化型耐热全息防伪膜 |
| WO2019168508A1 (en) * | 2018-02-27 | 2019-09-06 | MURIN, Peter, A. | Method and material for synthesis and purification by use of a coated solid substrate |
| WO2021162928A1 (en) * | 2020-02-11 | 2021-08-19 | University Of Florida Research Foundation | Water dispersible composite particles, methods of making, and coatings |
| US11839667B2 (en) | 2020-10-19 | 2023-12-12 | The Procter & Gamble Company | Oral care article comprising a delivery carrier and solid hydrophilic particles comprising an active agent |
| WO2022086526A1 (en) * | 2020-10-21 | 2022-04-28 | MURIN, Peter, A. | Method and material for specialized sponge in chemical reactions and extractions |
Family Cites Families (90)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3157635A (en) | 1960-08-05 | 1964-11-17 | Takeda Chemical Industries Ltd | Sulfonic acid cation exchange resin purification of nucleotides |
| US4399009A (en) * | 1981-01-19 | 1983-08-16 | Oronzio Denora Impianti Elettrochimici S.P.A. | Electrolytic cell and method |
| US4399235A (en) | 1981-05-11 | 1983-08-16 | The Dow Chemical Company | High density ion exchange resins |
| US4780367A (en) | 1983-06-27 | 1988-10-25 | Minnesota Mining And Manufacturing Company | Tackified star block copolymer pressure-sensitive adhesive composition and the sheet materials coated therewith |
| US4849311A (en) | 1986-09-24 | 1989-07-18 | Toa Nenryo Kogyo Kabushiki Kaisha | Immobilized electrolyte membrane |
| US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| US4923978A (en) | 1987-12-28 | 1990-05-08 | E. I. Du Pont De Nemours & Company | Process for purifying nucleic acids |
| EP0409432A3 (en) | 1989-07-20 | 1991-12-11 | Warner-Lambert Company | Confectionery delivery system |
| JP2978187B2 (ja) | 1989-11-02 | 1999-11-15 | 日本ケミカルリサーチ株式会社 | 修飾スーパーオキサイドディスムターゼの製造法 |
| US5187066A (en) | 1990-02-14 | 1993-02-16 | Syntex (U.S.A.) Inc. | Methods for detecting amphiphilic antigens |
| US5770029A (en) | 1996-07-30 | 1998-06-23 | Soane Biosciences | Integrated electrophoretic microdevices |
| EP0447362A1 (en) | 1990-03-13 | 1991-09-18 | Warner-Lambert Company | Improved ingestible anion exchange resin delivery system compositions containing adipic acid |
| US5334316A (en) | 1990-10-10 | 1994-08-02 | Brigham Young University | Process of using polytetraalkylammonium and polytrialkylamine-containing ligands bonded to inorganic supports for removing and concentrating desired ions from solutions |
| US5294668A (en) | 1990-11-15 | 1994-03-15 | Minnesota Mining And Manufacturing Company | Polyolefin pressure-sensitive adhesive compositions containing macromonomers |
| US5264184A (en) | 1991-03-19 | 1993-11-23 | Minnesota Mining And Manufacturing Company | Device and a method for separating liquid samples |
| EP0530357B1 (en) | 1991-03-21 | 1995-11-15 | Johnson & Johnson Clinical Diagnostics, Inc. | Element and method for nucleic acid amplification and detection using adhered probes |
| US6093558A (en) | 1991-07-25 | 2000-07-25 | Edge Biosystems, Inc. | Binding protein of biologically active compositions to an adhesive formulation on a substrate |
| US5380901A (en) | 1992-01-30 | 1995-01-10 | The United States Of America As Represented By The Secretary Of Commerce | Multifunctional acrylates and the synthesis thereof |
| US5183705A (en) | 1992-02-28 | 1993-02-02 | Minnesota Mining And Manufacturing Company | Pressure-sensitive adhesive composition having high shear strength |
| ATE161866T1 (de) * | 1992-10-21 | 1998-01-15 | Cornell Res Foundation Inc | Selektive, die porengrösse betreffende chemische modifikation poröser materialien |
| US6780818B2 (en) * | 1994-02-02 | 2004-08-24 | The Regents Of The University Of California | Quantitative organic vapor-particle sampler |
| US5670346A (en) | 1994-03-08 | 1997-09-23 | Amersham International Plc | Modifying nucleotide analogues |
| JP3546463B2 (ja) | 1994-03-31 | 2004-07-28 | 三菱化学株式会社 | 混合イオン交換樹脂床の分離用球状不活性樹脂 |
| SE9500183D0 (sv) * | 1995-01-20 | 1995-01-20 | Pharmacia Biotech Ab | Method for the purifivation of short nucleic acids |
| US6063838A (en) | 1995-02-16 | 2000-05-16 | 3M Innovative Properties Company | Blended pressure-sensitive adhesives |
| US5741828A (en) | 1995-05-01 | 1998-04-21 | S.K.Y. Polymers, Inc. | Flexible hydrophilic composite coatings |
| US6143247A (en) * | 1996-12-20 | 2000-11-07 | Gamera Bioscience Inc. | Affinity binding-based system for detecting particulates in a fluid |
| US6168948B1 (en) | 1995-06-29 | 2001-01-02 | Affymetrix, Inc. | Miniaturized genetic analysis systems and methods |
| US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US5700902A (en) | 1995-07-27 | 1997-12-23 | Circe Biomedical, Inc. | Block copolymers |
| US20010055812A1 (en) * | 1995-12-05 | 2001-12-27 | Alec Mian | Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics |
| JP3818689B2 (ja) | 1996-01-16 | 2006-09-06 | 富士写真フイルム株式会社 | コロイド状シリカをコアとし、有機ポリマーをシェルとするコア／シェル状複合粒子の水性分散物及びその製造方法 |
| CN1202260C (zh) | 1996-01-23 | 2005-05-18 | 佳根基因组学公司 | 利用大小分离技术分析核酸分子的方法和组合物 |
| FR2744803B1 (fr) | 1996-02-12 | 1998-03-13 | Bio Merieux | Procede et dispositif de traitement d'une carte d'analyse |
| US6074827A (en) | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
| WO1998012351A1 (de) | 1996-09-19 | 1998-03-26 | Roth W Kurt | Verfahren zur reinigung und gegebenenfalls analyse von nukleinsäuren aus biologischen proben |
| DE19731670C2 (de) | 1997-07-23 | 2000-06-29 | Dorothea Waschk | Verfahren zur Reinigung und gegebenenfalls Analyse von Nukleinsäuren aus biologischen Proben |
| DE69727244T2 (de) | 1996-10-03 | 2004-11-25 | Hermes Biosciences Inc., San Francisco | Hydrophile Mikroteilchen und Verfahren zu deren Herstellung |
| WO1998020960A1 (en) | 1996-11-12 | 1998-05-22 | Whatman, Inc. | Hydrophilic polymeric phase inversion membrane |
| US5997818A (en) | 1997-02-27 | 1999-12-07 | Minnesota Mining And Manufacturing Company | Cassette for tonometric calibration |
| SE9700769D0 (sv) | 1997-03-04 | 1997-03-04 | Pharmacia Biotech Ab | Matriser för separation och separation som utnyttjar matriserna |
| GB9709728D0 (en) * | 1997-05-13 | 1997-07-02 | Dynal As | Single step method |
| US6632399B1 (en) * | 1998-05-22 | 2003-10-14 | Tecan Trading Ag | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays |
| US6451260B1 (en) * | 1997-08-26 | 2002-09-17 | Dyax Corp. | Method for producing microporous elements, the microporous elements thus produced and uses thereof |
| AU744879B2 (en) | 1997-09-19 | 2002-03-07 | Aclara Biosciences, Inc. | Apparatus and method for transferring liquids |
| CA2301592A1 (en) | 1997-09-19 | 1999-04-01 | David S. Soane | Capillary electroflow apparatus and method |
| WO1999039120A1 (en) | 1998-01-29 | 1999-08-05 | University Of Pittsburgh | Thermal expansion-induced fluid control for microfluidic devices |
| AU757405B2 (en) | 1998-03-10 | 2003-02-20 | Bayer Aktiengesellschaft | Integrated assay device and methods of production and use |
| US6265168B1 (en) * | 1998-10-06 | 2001-07-24 | Transgenomic, Inc. | Apparatus and method for separating and purifying polynucleotides |
| US6534262B1 (en) | 1998-05-14 | 2003-03-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| US6103199A (en) | 1998-09-15 | 2000-08-15 | Aclara Biosciences, Inc. | Capillary electroflow apparatus and method |
| US6277488B1 (en) | 1998-10-28 | 2001-08-21 | 3M Innovative Properties Company | Adhesive composition containing a block copolymer composition and polyphenylene oxide resin and products thereof |
| US6240790B1 (en) * | 1998-11-09 | 2001-06-05 | Agilent Technologies, Inc. | Device for high throughout sample processing, analysis and collection, and methods of use thereof |
| WO2000045180A1 (en) | 1999-02-01 | 2000-08-03 | 3M Innovative Properties Company | Poly(alpha-olefin) adhesive cover tapes for analytical receptacles |
| AU3157800A (en) * | 1999-02-22 | 2000-09-14 | Evotec Biosystems Ag | Utilization of supporting material in capillary electrochromatography |
| US6306273B1 (en) | 1999-04-13 | 2001-10-23 | Aclara Biosciences, Inc. | Methods and compositions for conducting processes in microfluidic devices |
| WO2000068336A1 (en) | 1999-05-05 | 2000-11-16 | 3M Innovative Properties Company | Silicone adhesives, articles, and methods |
| GB9915398D0 (en) | 1999-07-02 | 1999-09-01 | Baker Matthew J | Magnetic particles |
| US6524456B1 (en) | 1999-08-12 | 2003-02-25 | Ut-Battelle, Llc | Microfluidic devices for the controlled manipulation of small volumes |
| US6383783B1 (en) | 1999-09-21 | 2002-05-07 | 3M Innovative Properties Company | Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant |
| US6414136B1 (en) | 1999-10-06 | 2002-07-02 | Prolinx, Inc. | Removal of dye-labeled dideoxy terminators from DNA sequencing reactions |
| GB9927904D0 (en) | 1999-11-25 | 2000-01-26 | Amersham Pharm Biotech Ab | A method fro obtaining a nucleic acid variant |
| CA2290731A1 (en) | 1999-11-26 | 2001-05-26 | D. Jed Harrison | Apparatus and method for trapping bead based reagents within microfluidic analysis system |
| US6875348B2 (en) * | 2000-02-18 | 2005-04-05 | The Board Of Trustees Of The Leland Stanford Junior University | Separation column having a photopolymerized sol-gel component and associated methods |
| AU2001238616A1 (en) | 2000-02-23 | 2001-09-03 | Wen Shao | Rapid nucleic acid separation, isolation and purification methods |
| US6454924B2 (en) * | 2000-02-23 | 2002-09-24 | Zyomyx, Inc. | Microfluidic devices and methods |
| WO2001068913A2 (en) | 2000-03-13 | 2001-09-20 | Genset | Nucleic acid detection method and system |
| EP1274503A2 (en) | 2000-03-14 | 2003-01-15 | Hammen Corporation | Composite matrices with interstitial polymer networks |
| DE60137203D1 (de) | 2000-03-24 | 2009-02-12 | Qiagen Gmbh | Poröse ferro- oder ferrimagnetische glasteilchen für molekültrennung |
| US6720187B2 (en) | 2000-06-28 | 2004-04-13 | 3M Innovative Properties Company | Multi-format sample processing devices |
| US6734401B2 (en) | 2000-06-28 | 2004-05-11 | 3M Innovative Properties Company | Enhanced sample processing devices, systems and methods |
| US6627159B1 (en) | 2000-06-28 | 2003-09-30 | 3M Innovative Properties Company | Centrifugal filling of sample processing devices |
| US6504021B2 (en) | 2000-07-05 | 2003-01-07 | Edge Biosystems, Inc. | Ion exchange method for DNA purification |
| US6706661B1 (en) * | 2000-09-01 | 2004-03-16 | Exxonmobil Research And Engineering Company | Fischer-Tropsch catalyst enhancement |
| WO2002046398A2 (en) * | 2000-11-06 | 2002-06-13 | The University Of Houston System | Nucleic acid separation using immobilized metal affinity chromatography |
| US20030017567A1 (en) | 2001-04-24 | 2003-01-23 | 3M Innovative Properties Company | Biological sample processing methods and compositions that include surfactants |
| US6617136B2 (en) | 2001-04-24 | 2003-09-09 | 3M Innovative Properties Company | Biological sample processing methods and compositions that include surfactants |
| US7374724B2 (en) * | 2001-05-29 | 2008-05-20 | Tecan Trading Ag | Device for processing samples, use of the device, and method for producing the device |
| US6682702B2 (en) * | 2001-08-24 | 2004-01-27 | Agilent Technologies, Inc. | Apparatus and method for simultaneously conducting multiple chemical reactions |
| ATE477054T1 (de) * | 2001-09-17 | 2010-08-15 | Gyros Patent Ab | Einen kontrollierten strom in einer mikrofluidvorrichtung ermöglichende funktionseinheit |
| US7347976B2 (en) * | 2001-12-20 | 2008-03-25 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix |
| US7192560B2 (en) * | 2001-12-20 | 2007-03-20 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
| US6833238B2 (en) | 2002-01-04 | 2004-12-21 | Applera Corporation | Petal-array support for use with microplates |
| US20040023220A1 (en) | 2002-07-23 | 2004-02-05 | Lawrence Greenfield | Integrated method for PCR cleanup and oligonucleotide removal |
| WO2004010760A2 (en) | 2002-07-26 | 2004-02-05 | Applera Corporation | Microfluidic size-exclusion devices, systems, and methods |
| EP1525299A2 (en) | 2002-07-26 | 2005-04-27 | Applera Corporation | Petal-array support for use with microplates |
| US7214348B2 (en) | 2002-07-26 | 2007-05-08 | Applera Corporation | Microfluidic size-exclusion devices, systems, and methods |
| US20040018559A1 (en) | 2002-07-26 | 2004-01-29 | Applera Corporation | Size-exclusion ion-exchange particles |
| EP1552013A1 (en) | 2002-07-26 | 2005-07-13 | Applera Corporation | Microfluidic device including purification column with excess diluent, and method |
| US7981600B2 (en) | 2003-04-17 | 2011-07-19 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene |
-
2001
- 2001-12-20 US US10/027,226 patent/US7347976B2/en not_active Expired - Fee Related
-
2002
- 2002-11-26 AU AU2002359526A patent/AU2002359526A1/en not_active Abandoned
- 2002-11-26 CA CA002469999A patent/CA2469999A1/en not_active Abandoned
- 2002-11-26 JP JP2003555174A patent/JP2006501805A/ja active Pending
- 2002-11-26 EP EP02794069A patent/EP1458485A4/en not_active Withdrawn
- 2002-11-26 WO PCT/US2002/038143 patent/WO2003054510A2/en not_active Ceased
-
2007
- 2007-11-09 US US11/937,567 patent/US20080103297A1/en not_active Abandoned
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8164828B2 (en) | 2006-12-26 | 2012-04-24 | Nsk Ltd. | Observable centrifugal apparatus and observation apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2469999A1 (en) | 2003-07-03 |
| EP1458485A2 (en) | 2004-09-22 |
| EP1458485A4 (en) | 2005-03-16 |
| US7347976B2 (en) | 2008-03-25 |
| WO2003054510A2 (en) | 2003-07-03 |
| WO2003054510A3 (en) | 2004-02-19 |
| US20030120062A1 (en) | 2003-06-26 |
| AU2002359526A1 (en) | 2003-07-09 |
| US20080103297A1 (en) | 2008-05-01 |
| AU2002359526A8 (en) | 2003-07-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7347976B2 (en) | Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix | |
| US7192560B2 (en) | Methods and devices for removal of organic molecules from biological mixtures using anion exchange | |
| US7981600B2 (en) | Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene | |
| EP1972688B1 (en) | Method of amplifying nucleic acid from microorganism using nonplanar solid substrate | |
| US20100233696A1 (en) | Methods, flow cells and systems for single cell analysis | |
| US20140179909A1 (en) | Microfluidic device for nucleic acid extraction and fractionation | |
| WO2000050870A1 (en) | Biochemical purification devices with immobilized capture probes and their uses | |
| US20090215124A1 (en) | Nucleic acid isolation methods and materials and devices thereof | |
| US20090203083A1 (en) | Substrate for nucleic acid amplification | |
| JP7667739B2 (ja) | 固体支持体上で固定化されたｃｒｉｓｐｒ／ｃａｓ９を使用する核酸シークエンシングライブラリを調製するための組成物及び方法 | |
| WO2004050910A1 (en) | Method for hybridisation of immobilized genomic dna | |
| KR100813265B1 (ko) | 비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 증폭하는 방법 | |
| KR100813264B1 (ko) | 비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 증폭하는 방법 | |
| HK1130842A (en) | Substrate for nucleic acid amplification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20051125 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20051125 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20080708 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20081202 |