JP2006335758A - Composition for diet - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a high safety composition for diet derived from a raw coffee bean by which superior diet effect can be obtained, and which is useful for prevention and treatment of life cycle-related diseases such as diabetes. <P>SOLUTION: The composition for diet of the invention is characterised by containing a polar solvent extract of a degreased coffee bean as an effective component. The polar solvent extract is preferably a hydrous ethanol extract, more preferably a hydrous ethanol extract with a 40-90% (wt/wt) ethanol. The degreased raw coffee bean is preferably a raw coffee bean of which the oil component is extracted and separated with N-hexane. The composition for diet of the invention is preferably combindedly used together with any one kind or more kinds of Salacia extract, Oensothera tetraptera extract, Sesamin and Galcinia. The composition for diet of the invention can be used as a raw material for food and drink, medicine, or dermal outer-use agent. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a diet composition derived from green coffee beans, and is applied, for example, as a raw material for foods, drinks, medicines, cosmetics and the like.

  Obesity develops due to overeating or lack of exercise, and is considered a risk factor for lifestyle-related diseases such as diabetes. The total number of diabetic patients and so-called diabetic reserves that may develop in the future is on the rise, and eliminating obesity is essential for their treatment and prevention.

  Known diet materials include capsaicin that promotes fat metabolism, chitosan that reduces fat absorption, and citrus aurantium that promotes lipolysis, and these diet materials eliminate cellulite and water overweight. It is formulated in foods that support slimming from the cosmetic aspect, such as body fat reduction. However, some of these diet materials do not provide a sufficient diet effect, and the current situation is that high value-added diet materials useful for the treatment and prevention of lifestyle-related diseases are required in the market.

As prior art related to the diet material, Patent Literature 1, Patent Literature 2, and the like are disclosed.
JP 2003-34636 A JP 2002-308766 A Tholon L, et al., An in vitro, ex vivo, and in vivo demonstration of the lipolytic effect of slimming molecules: An unexpected alpha (2) -adrenergic antagonism. J. Cosmet. Sci. 53, 209-18 (2002) .

  Under such a background, the present inventors investigated the components / contents, SOD-like activity and the like of various plant-derived extracts, and “raw coffee beans containing abundant chlorogenic acids and caffeine”. It came to pay attention to. As a result of various experiments, it has been found that a component having an extremely high diet effect is contained in an extract of fresh coffee beans, particularly a polar solvent extract of defatted fresh coffee beans. In addition, as a novel physiological activity of the raw coffee bean extract, we found a fat absorption inhibitory effect that was not revealed so far, and also involved pancreatic lipase activity inhibitory activity involved in fat absorption metabolism and fat burning metabolism The present inventors have discovered the carnitine palmitoyltransferase activity promoting action and the α-glucosidase activity inhibiting action involved in suppressing the increase in blood glucose level, and have completed the present invention.

An object of the present invention is to provide a highly safe composition for diet derived from raw coffee beans, a composition for suppressing fat absorption, which can obtain an excellent diet effect and is useful for the prevention and treatment of lifestyle-related diseases such as diabetes. It is to provide a composition for inhibiting pancreatic lipase activity, a composition for promoting carnitine palmitoyltransferase activity, and a composition for inhibiting α-glucosidase activity.
Moreover, the other objective of this invention is to provide the food-drinks, chemical | medical agent, and skin external preparation for diet derived from raw coffee beans.
Furthermore, another object of the present invention is to provide a food and beverage product and a medicine derived from green coffee beans having a fat absorption suppressing action, a pancreatic lipase activity inhibiting action, a carnitine palmitoyltransferase activity promoting action, or an α-glucosidase activity inhibiting action. There is.

[First invention]
The composition for diet of this invention for solving the said subject contains the polar solvent extract of a defatted raw coffee bean, It is characterized by the above-mentioned.
The diet composition of the present invention is characterized in that the polar solvent extract is a hydrous ethanol extract.
The diet composition of the present invention is characterized in that the polar solvent extract is a hydrous ethanol extract having an ethanol concentration of 40 to 90% (wt / wt).
The diet composition of the present invention is characterized in that the defatted raw coffee beans are obtained by extracting and separating the oil content of raw coffee beans with N-hexane.
Furthermore, the diet composition of the present invention is characterized in that any one or more of Salacia extract, evening primrose extract, sesamin, garcinia is added to the diet composition described above. To do.

As a main factor of obesity, it is conceivable that fat is excessively stagnated in the fat metabolism pathway in the body (see FIG. 11). Therefore, to eliminate obesity targeting fat, that is, as a diet countermeasure, in the fat metabolism pathway, (1) suppression of fat absorption, (2) inhibition of fat accumulation, (3) promotion of lipolysis, (4) promotion of fat burning It is effective to perform effectively.
According to the present invention (first invention), by taking an excellent diet functional ingredient as a safe raw coffee bean-derived extract, (1) fat absorption inhibition, (2) fat accumulation inhibition, (3) fat All effects of promoting decomposition and (4) promoting fat burning can be obtained in a balanced manner. Thereby, the weight increase at the time of fat intake can be suppressed and an effective diet can be performed.

Here, Patent Documents 1 and 2 describe a lipid metabolism improving agent (Patent Document 1) and a lifestyle-related disease preventing / ameliorating agent (Patent Document 2) focusing on the function of chlorogenic acid contained in coffee beans. In addition, it is disclosed that an extract of green coffee beans has an anti-obesity effect.
However, the diet composition according to the present invention is obtained by extracting “degreased raw coffee beans” with a polar solvent and concentrating bioactive components derived from raw coffee beans containing chlorogenic acid to a high concentration. It differs from the composition obtained by extracting the beans as they are. And the polar solvent extract of defatted raw coffee beans is rich in excellent diet function components other than chlorogenic acid. Thereby, a very high diet effect (weight gain inhibitory effect) can be acquired compared with a non-defatted raw coffee bean extract.
According to the anti-obesity test of Patent Document 1, when comparing the weight gain inhibitory action of fresh coffee bean extract and chlorogenic acid, the case of taking chlorogenic acid alone rather than taking fresh coffee bean extract (See Example 5, Table 9 “Group 3 and Group 4” of Patent Document 1). According to the polar solvent extract of defatted green coffee beans according to the present invention, it is possible to expect a weight gain suppression effect superior to when chlorogenic acid is ingested alone. Such an excellent effect is an original effect that cannot be predicted from Patent Documents 1 and 2 and the like.

[Second Invention and Third Invention]
The composition for inhibiting fat absorption of the present invention (second invention) is characterized by containing a raw coffee bean extract as an active ingredient.
Moreover, the composition for fat absorption suppression of the present invention (second invention) is characterized in that the raw coffee bean extract is a polar solvent extract of defatted raw coffee beans.
Moreover, the composition for fat absorption suppression of the present invention (second invention) is characterized in that the polar solvent extract is a hydrous ethanol extract.
Moreover, the composition for fat absorption suppression of the present invention (second invention) is characterized in that the polar solvent extract is a hydrous ethanol extract having an ethanol concentration of 40 to 90% (wt / wt).
Furthermore, the composition for fat absorption inhibition of the present invention (second invention) is characterized in that the defatted raw coffee beans are obtained by extracting and separating the oil content of the raw coffee beans with N-hexane.

The pancreatic lipase activity inhibitory composition of the present invention (third invention) is characterized by containing a raw coffee bean extract as an active ingredient.
In the pancreatic lipase activity inhibitory composition of the present invention (third invention), the fresh coffee bean extract is a polar solvent extract of defatted fresh coffee beans.
The pancreatic lipase activity inhibitory composition of the present invention (third invention) is characterized in that the polar solvent extract is a hydrous ethanol extract.
The pancreatic lipase activity inhibitory composition of the present invention (third invention) is characterized in that the polar solvent extract is a hydrous ethanol extract having an ethanol concentration of 40 to 90% (wt / wt).
Furthermore, the composition for inhibiting pancreatic lipase activity of the present invention (second invention) is characterized in that the defatted raw coffee beans are obtained by extracting and separating the oil content of raw coffee beans with N-hexane.

  As described above, when evaluating the diet effect targeting fat, (1) fat absorption inhibition, (2) fat accumulation inhibition, (3) fat decomposition promotion, (4) fat burning promotion in the fat metabolism pathway Often, one of these effects is examined. In Patent Documents 1 and 2, the triglyceride level in blood is measured to clarify the lipolysis promoting action, suggesting the anti-obesity action of fresh coffee bean extract. However, it is hardly known that the raw coffee bean extract has a fat absorption inhibitory action when ingesting fat.

  The present inventors have tested the physiological activity of fresh coffee bean extract in a fat absorption system in fat metabolism, and have come to find out its fat absorption inhibitory action and pancreatic lipase inhibitory activity action involved in fat absorption metabolism. That is, according to the present invention, the diet effect can be improved by the fat absorption inhibitory action (second invention) and pancreatic lipase inhibitory activity action (third invention) of the raw coffee bean extract. In other words, a diet composition containing a raw coffee bean extract can be provided as an active ingredient for fat absorption inhibitory action or as an active ingredient for pancreatic lipase inhibitory activity. And as an active ingredient of these actions, the polar solvent extract (including ethanol extract and N-hexane defatted product) of the defatted raw coffee beans described in the second invention or the third invention described above can be used.

[Fourth Invention]
The composition for promoting carnitine palmitoyltransferase activity of the present invention (fourth invention) is characterized by containing a raw coffee bean extract as an active ingredient.
The composition for promoting carnitine palmitoyltransferase activity of the present invention (fourth invention) is characterized in that the raw coffee bean extract is a polar solvent extract of defatted raw coffee beans.
In the composition for promoting carnitine palmitoyltransferase activity of the present invention (fourth invention), the polar solvent extract is a hydrous ethanol extract.
The composition for promoting carnitine palmitoyltransferase activity of the present invention (fourth invention) is characterized in that the polar solvent extract is a hydrous ethanol extract having an ethanol concentration of 40 to 90% (wt / wt).
Furthermore, the composition for promoting carnitine palmitoyltransferase activity of the present invention (fourth invention) is characterized in that the defatted raw coffee beans are obtained by extracting and separating the oil content of raw coffee beans with N-hexane.

Brown adipose tissue involved in fat metabolism is one of the fat-burning tissues that can convert energy into heat and dissipate it. Heat production is performed on the brown adipocyte mitochondrial membrane, and it is known that a special uncoupling protein (UCP-1) functions during heat conversion.
Caffeine contained in raw coffee beans has been reported to promote the expression of UCP-1 in the brown adipose tissue of diabetic mice. From this result, raw coffee beans containing caffeine burn fat in the form of heat production. It is thought that there is work to consume.

  On the other hand, a part of the fatty acid decomposed and released from the adipose tissue is carried to the liver, where it undergoes β-oxidation and is metabolized in the mitochondria of hepatocytes. When fatty acids are transported to mitochondria, carnitine and carnitine palmitoyltransferase (CPT), which is a transfer enzyme, function, and these are the rate-limiting step in β-oxidation.

  According to Patent Document 1, it has been clarified that chlorogenic acids contained in coffee beans activate gene transcription of enzymes important for fatty acid metabolism such as β-oxidation. The effect on activity is not mentioned.

  The present inventors tested the physiological activity of fresh coffee bean extract in a fat burning system in fat metabolism, and came to know the activation action of carnitine palmitoyltransferase (CPT) involved in β-oxidation. That is, according to the present invention (fourth invention), the diet effect can be improved in terms of promoting fat burning by activating carnitine palmitoyltransferase (CPT). In other words, it is possible to provide a diet composition containing a raw coffee bean extract as an active ingredient for the activating action of carnitine palmitoyltransferase (CPT). And as an active ingredient of carnitine palmitoyltransferase (CPT) activating action, the polar solvent extract (including ethanol extract and N-hexane defatted product) of the defatted raw coffee beans described in the fourth invention described above is used. it can.

[Fifth Invention]
The composition for inhibiting α-glucosidase activity of the present invention (the fifth invention) is characterized by containing a raw coffee bean extract as an active ingredient.
In the composition for inhibiting α-glucosidase activity of the present invention (the fifth invention), the fresh coffee bean extract is a polar solvent extract of defatted fresh coffee beans.
The composition for inhibiting α-glucosidase activity of the present invention (the fifth invention) is characterized in that the polar solvent extract is a hydrous ethanol extract.
The composition for inhibiting α-glucosidase activity of the present invention (the fifth invention) is characterized in that the polar solvent extract is a hydrous ethanol extract having an ethanol concentration of 40 to 90% (wt / wt).
Furthermore, the composition for inhibiting α-glucosidase activity of the present invention (the fifth invention) is characterized in that the defatted raw coffee beans are obtained by extracting and separating the oil content of raw coffee beans with N-hexane.

  As described above, according to Patent Document 1, the transcriptional activation effect of the lipid metabolic enzyme gene is clarified with respect to chlorogenic acid contained in green coffee beans, and the blood glucose level increase inhibiting action of chlorogenic acid is disclosed. However, the physiological activity of the raw coffee bean extract on glycolytic enzymes has not been clarified.

  The present inventors tested the raw coffee bean extract for physiological activity against glycolytic enzymes such as α-amylase and α-glucosidase, and among these glycolytic enzymes, in particular, raw coffee against α-glucosidase. It was found that the bean extract has an excellent activity inhibitory action. That is, this invention (5th invention) can suppress the raise of a postprandial blood glucose level by inhibiting the enzyme activity of (alpha) -glucosidase, and can prevent diabetes and obesity effectively. In other words, it is possible to provide an anti-diabetic composition or a diet composition containing a raw coffee bean extract as an active ingredient of an α-glucosidase activity inhibitory action. The polar solvent extract (including ethanol extract and N-hexane defatted product) of the defatted raw coffee bean described in the fifth invention can be used as an active ingredient of the α-glucosidase activity inhibiting action.

  Hereinafter, embodiments of the composition for diet according to the present invention (first invention) will be described. The following embodiments are the second invention (composition for suppressing fat absorption), the third invention (composition for inhibiting pancreatic lipase activity). Product), the fourth invention (composition for promoting carnitine palmitoyltransferase activity), and the fifth invention (composition for inhibiting α-glucosidase activity).

  As the raw coffee beans that are the raw material of the diet composition of the present invention, coffee beans for beverages can be used. Coffee beans for beverage are usually roasted at a maximum of 200 to 215 ° C. for about 15 minutes, but in the present invention, raw beans are used instead of roasted beans.

  The coffee bean plant (coffee tree) is an evergreen shrub of Rubiaceae native to Ethiopia, usually containing two seeds, the seeds are hemispherical and have a deep groove on a flat surface. (Coffea arabica L.), C. robusta Linden, C. liberica Bull., Etc. are cultivated. In the present invention, the type of coffee tree is not limited, and the production region of coffee beans (Arabica, Robusta, etc.) is not limited.

  In addition, as a method of preparing coffee beans for beverages, there are a dry method in which the fruit is dried and the pulp and the outer skin are removed, and a wet method in which the fruit is removed by fermenting by soaking in water and then dried to remove the outer skin. However, in the present invention, green coffee beans by any adjustment method can be used.

  It is desirable to use defatted raw coffee beans as an extraction raw material for the diet composition of the present invention. This is because by removing the oil in the green coffee beans, the diet functional component is easily extracted from the defatted product. As a degreasing method, for example, after squeezing green coffee beans to separate oil, the residual oil in the pressed product may be extracted and separated with a degreasing solvent (fat-soluble organic solvent). In addition, when implementing the above-mentioned 2nd invention-5th invention, you may use a non-defatted fresh coffee bean. For example, raw coffee beans can be crushed, and the active ingredients can be solvent extracted from the crushed material.

  Preferred degreasing solvents include N-hexane, acetone and the like. In particular, when N-hexane is used as a degreasing solvent, the extracted oil can be used as an edible oil, and the extract of defatted raw coffee beans can be easily used as a food material.

As a polar solvent for extracting diet functional components from defatted raw coffee beans, water, methanol, ethanol, isopropyl alcohol, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, ethyl acetate, etc. may be used. it can. Two or more of these solvents may be mixed.
Desirably, when water or ethanol is used as the extraction solvent, the active ingredient is efficiently extracted. In particular, hydrous ethanol is an extraction solvent that is less likely to reduce the activity of the active ingredient during extraction and is preferable from the standpoint of safety in the use of the extract in food. The kind of water for extraction is not particularly limited, and tap water, distilled water, mineral water, alkaline ionized water, deep water and the like can be used. In addition, when implementing the above-mentioned 2nd invention-5th invention, an active ingredient can be extracted even if it uses a nonpolar solvent (acetone etc.).

  As extraction temperature which extracts a diet functional component from defatted raw coffee beans, when using water-containing ethanol, for example, it is good to carry out at extraction temperature 20-80 degreeC, desirably about 40-50 degreeC. This is because if the extraction temperature is too low, it becomes difficult to extract the active ingredient, and if the extraction temperature is too high, the activity of the active ingredient tends to decrease.

  The water-containing ethanol as the extraction solvent has an ethanol concentration of 40 to 90% (wt / wt), desirably an ethanol concentration of 60 to 80% (wt / wt). The reason why the ethanol concentration is 40% (wt / wt) or more is that if the ethanol content is too small, the extraction amount of the active ingredient tends to be insufficient. The reason why the ethanol concentration is 90% (wt / wt) or less is that if the ethanol concentration is too high, the residual oil content of the defatted raw coffee beans is likely to dissolve into the water-containing ethanol. In addition, in order to improve the content rate of the active ingredient, the water-containing ethanol extraction may be repeatedly performed while changing the ethanol concentration stepwise.

  As a method for extracting a diet functional component, any method such as continuous extraction, immersion extraction, countercurrent extraction, and supercritical extraction can be employed, and any apparatus can be used at room temperature or under reflux heating.

Specifically, an extraction raw material (defatted raw coffee beans) is put into a treatment tank filled with an extraction solvent, and an active ingredient is eluted while stirring. For example, when water-containing ethanol is used as the extraction solvent, the extraction solvent is used in an amount of about 5 to 100 times (weight ratio) of the extraction raw material, and extraction is performed for about 30 minutes to 2 hours. The active ingredient is eluted in the solvent, and then filtered to remove the extraction residue to obtain an extract. Thereafter, the extract is subjected to treatments such as dilution, concentration, drying, and purification according to a conventional method to obtain a diet composition according to the present invention.
Examples of the purification method include activated carbon treatment, resin adsorption treatment, ion exchange resin, liquid-liquid countercurrent distribution, and the like, but they are not used in large quantities when added to foods. It may be used unpurified.

  According to the inventors' investigation, the polar solvent extract of defatted raw coffee beans contains a relatively large amount of chlorogenic acids and caffeine. In particular, specific chlorogenic acids excellent in diet function are concentrated. The As for these contents, when chlorogenic acid is contained in the extract at a ratio of 20 wt% or more and chlorogenic acids (chlorogenic acid, ferulic acid, p-coumaric acid, caffeic acid, etc.) are contained in an extract of 45 wt% or more, the diet effect Will improve dramatically.

  The composition for diet of this invention can be used as a raw material of various food-drinks. Examples of food and drink include confectionery (gum, candy, caramel, chocolate, cookies, snacks, jelly, gummi, tablet confectionery, etc.), noodles (soba, udon, ramen, etc.), and dairy products (milk, ice cream, yogurt). ), Seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, tea, tea, carbonated beverages, sports drinks, etc.), health foods (tablets, capsules, etc.), nutrition Supplementary foods (such as energy drinks) are listed. The diet composition of the present invention may be appropriately blended with these foods and drinks.

  These foods and drinks can be blended with various ingredients depending on the type, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid. Acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, Food materials such as carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances and preservatives can be used.

  As a specific manufacturing method, a polar solvent extract of defatted green coffee beans is spray-dried or freeze-dried together with powdered cellulose, and this is easily converted into powder, granules, tableting or solution, so that foods and drinks (instant foods, etc.) can be easily obtained. Can be contained. Moreover, the polar solvent extract of defatted raw coffee beans can be dissolved in, for example, fats and oils, ethanol, glycerin, or a mixture thereof, and added to beverages or solid foods. If necessary, it can be mixed with a binder such as gum arabic or dextrin to form a powder or granules and added to a beverage or a solid food.

  As the addition amount when the diet composition of the present invention is applied to foods and drinks, since the main purpose is disease prevention and health maintenance, the content of active ingredients in the foods and drinks is 1 to 20 wt% or less in total. Preferably there is.

  You may use the composition for diet of this invention as a raw material of a chemical | medical agent (a pharmaceutical and a quasi-drug are included). It can be produced by appropriately blending the diet composition of the present invention with a raw material for a pharmaceutical preparation. Examples of the preparation raw material that can be blended in the diet composition of the present invention include excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cacao butter, hydrogenated vegetable oil, kaolin, talc, and the like. Etc.), binder (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrant (sodium alginate, agar, hydrogen carbonate) Sodium, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, starch, lactose, gum arabic powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers (fourth) Quaternary ammonium base, sodium lauryl sulfate Etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), lubricants (purified talc, stearates, polyethylene glycol, and the like) and the like.

  The method of administration of the diet composition according to the present invention can generally be administered orally in the form of tablets, pills, soft / hard capsules, fine granules, powders, granules, liquids, etc. It may be administered parenterally. When administered as a parenteral agent, it can be administered as a solution or with the addition of a dispersing agent, suspension, stabilizer, etc., by local tissue administration, intradermal, subcutaneous, intramuscular and intravenous injection, etc. it can. Moreover, it is good also as forms, such as a suppository.

The dosage may vary depending on the administration method, medical condition, age of the patient, etc., but for adults, it can be generally administered as 0.5 to 5000 mg as an active ingredient per day, and usually about 0.5 to 3000 mg for children.
The mixing ratio of the diet composition can be appropriately changed depending on the dosage form, but is usually about 0.3 to 15.0 wt% when administered orally or by mucosal absorption, and when administered parenterally. About 0.01 to 10 wt% is preferable. In addition, since the dose varies depending on various conditions, a dose smaller than the above dose may be sufficient, or it may be necessary to administer beyond the range.

  The diet composition of the present invention contains chlorogenic acids and caffeine, but there is a report that a combined preparation composed of caffeine and several components showed a slimming effect when applied externally (Non-patent Document 1). From these results, the diet composition of the present invention can be expected to have a diet effect even when used as a skin external preparation (including cosmetics, pharmaceuticals, and quasi-drugs) having a partial thinness and cellulite-resolving action. .

Cosmetic forms into which the diet composition of the present invention can be formulated include, for example, emulsions, soaps, facial cleansers, bath preparations, creams, emulsions, lotions, eau de cologne, shaving creams, shaving lotions, cosmetic oils, Sunscreen / sunscreen lotion, funny powder, foundation, perfume, pack, nail cream, enamel, enamel remover, eyebrow, blusher, eye cream, eye shadow, mascara, eyeliner, lipstick, lip balm, shampoo, rinse, hair dye , Dispersions, cleaning materials, and the like.
In addition, examples of the form of the pharmaceutical or quasi-drug that can be mixed with the diet composition of the present invention include ointments, creams, and liquids for external use.

In addition to the composition for diet according to the present invention, the external preparation for skin of the above-described form includes components, oils, higher alcohols, and the like, which are blended in external preparations for skin such as cosmetics and quasi-drugs, as long as the diet effect is not impaired. Fatty acids, ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, and the like can be blended.
Examples are listed below, but the present invention is not limited to these examples.
(1) Examples of oil [ester-based oil phase components]: glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, Isocetyl isostearate, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate , Diisopropyl adipate, isoaralkyl neopentanoate, glyceryl tri (capryl / caprate), trimethylolpropane tri-2-ethylhexanoate, trimethylotriisostearate Propane, tetra-2-ethylhexanoate pentaerythritol, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isolaurate Stearyl, isotridecyl myristate, isocetyl myristate, isostearyl myristate, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl linoleate, octyldodecyl isoleate, isopropyl isostearate Cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate Cole, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol di (capryl / capric acid), propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, triisopalmitine Glyceryl acid, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyl decyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleic acid Ester, polyglycerol isostearate, dipropyl carbonate Dialkyl carbonate (C12-18), triisocetyl citrate, triisoaralkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyl decyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate , Trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, Cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, 12-stearoylhydroxystearin Isocetyl, 12-stearoyl-hydroxystearic acid stearyl 12-stearoyl-hydroxystearic acid isostearate, and the like.
[Hydrocarbon-based oil phase component]: Squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum and the like.
Animal and vegetable oils and their hydrogenated oils, and naturally occurring waxes: beef tallow, hydrogenated beef tallow, lard, hydrogenated tallow, horse oil, hydrogenated horse oil, mink oil, orange luffy oil, fish oil, hydrogenated fish oil, egg yolk oil and other animal oils and Its hardened oil, avocado oil, almond oil, olive oil, cacao butter, apricot kernel oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil, perilla oil , Tea seed oil, camellia oil, corn oil, rapeseed oil, hardened rapeseed oil, palm kernel oil, hardened palm kernel oil, palm oil, hardened palm oil, peanut oil, hardened peanut oil, castor oil, hardened castor oil, sunflower oil Vegetable oil such as grape seed oil, jojoba oil, hardened jojoba oil, macadamia nut oil, medhome oil, cottonseed oil, hardened cottonseed oil, coconut oil, hardened coconut oil, and its hardened oil, beeswax, high Valence beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax and montan wax.
[Silicone-based oil phase component]: dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane , Dimethylsiloxane / methylcetyloxysiloxane copolymer, dimethylsiloxane / methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel , Acrylic silicone, trimethylsiloxysilicic acid, silicone RTV rubber and the like.
[Fluorine oil phase component]: Perfluoropolyether, fluorine-modified organopolysiloxane, fluorinated pitch, fluorocarbon, fluoroalcohol, fluoroalkyl / polyoxyalkylene co-modified organopolysiloxane, and the like.
(2) Examples of higher alcohols Lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, octyldodecanol and the like can be mentioned.
(3) Examples of fatty acids Caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid , Erucic acid, 2-ethylhexanoic acid and the like.
(4) Examples of UV absorbers Paraaminobenzoic acid, amyl paraaminobenzoate, ethyldihydroxypropyl paraaminobenzoate, glyceryl paraaminobenzoate, ethyl paraaminobenzoate, octyl paraaminobenzoate, octyldimethyl paraaminobenzoate, ethylene glycol salicylate, salicylic acid Octyl, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl paramethoxycinnamate, 2-ethylhexyl paramethoxycinnamate, mono-2-diparamethoxycinnamate Glyceryl ethylhexanoate, isopropyl paramethoxycinnamate, diethanolamine salt of paramethoxyhydrocinnamic acid, diisopropyl diisopropylcinnamic acid Stealth mixture, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, Butylmethoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2-ethylhexyl-1-oxy) -1,3,5-triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole , Methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenyl acrylate, phenylbenzimidazole sulfate, 3- (4-methylbenzylidene) camphor, Isopropyl dibenzoylmethane, 4- (3,4-dimethoxyphenyl methylene) 2-ethylhexyl-2,5-dioxo-1-imidazolidin propionate,, and polymer derivatives and silane derivatives thereof.
(5) Examples of powders and pigments Red 104, Red 201, Yellow 4, Blue 1, Black 401 and other dyes, Yellow 4 AL lake, Yellow 203 BA lake and other lake dyes, nylon powder , Silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polyethylene powder, yellow iron oxide, red iron oxide, black Colored pigments such as iron oxide, chromium oxide, carbon black, ultramarine and bitumen, white pigments such as zinc oxide, titanium oxide and cerium oxide, extender pigments such as talc, mica, sericite, kaolin and barium sulfate plate, titanium mica Pearl pigments such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, metal salts such as magnesium silicate, Inorganic powders such as Rica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, zinc undecylenate, bentonite, smectite, boron nitride, etc. It is done. There are no particular restrictions on the shape of these powders (spherical, rod-like, needle-like, plate-like, irregular shape, flake-like, spindle-like, etc.) and particle diameter. These powders are conventionally known surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil agent treatment, N-acylated lysine treatment, The surface treatment may or may not be performed in advance by polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment or the like.
(6) Examples of surfactant [anionic surfactant]: fatty acid soap, α-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether Sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphates, alkylamide phosphates, alkyloyl alkyl taurine salts, N-acyl amino acid salts, POE alkyl ether carboxylates, alkyl sulfosuccinates, alkyls Examples include sodium sulfoacetate, acylated hydrolyzed collagen peptide salt, and perfluoroalkyl phosphate.
[Cationic surfactant]: alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzaza chloride Examples thereof include luconium, amidopropyldimethylhydroxypropylammonium chloride behenate, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, and lanolin derivative quaternary ammonium salts.
[Amphoteric surfactant]: Carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidoamine type and the like.
[Nonionic surfactant]: Propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE sorbitol fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE Examples thereof include hydrogenated castor oil, POE castor oil, POE / POP copolymer, POE / POP alkyl ether, polyether-modified silicone lauric acid alkanolamide, alkylamine oxide, and hydrogenated soybean phospholipid.
[Natural surfactant]: Examples include lecithin, saponin, and sugar surfactant.
(7) Examples of polyhydric alcohols and sugars Ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1,3 -Butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugar, sulfated trehalose, pullulan and the like. These chemically modified compounds can also be used.
(8) Examples of polymers Acrylic ester / methacrylic acid ester copolymer (plus size, manufactured by Kyoyo Chemical), vinyl acetate / crotonic acid copolymer (resin 28-1310, manufactured by NSC), vinyl acetate / croton Acid / vinyl neodecanate copolymer (28-2930, manufactured by NSC), methyl vinyl ether maleic acid half ester (Gantrez ES, manufactured by ISP), T-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (rubymer) , BASF), vinyl pyrrolidone / vinyl acetate / vinyl propionate copolymer (Rubicol VAP, BASF), vinyl acetate / crotonic acid copolymer (Rubiset CA, BASF), vinyl acetate / croton Acid / vinyl pyrrolidone copolymer (Rubiset CAP, manufactured by BASF), vinyl pyrrolidone / acrylate copolymer (Rubiflex, BA SF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxy vinyl polymer (Carbopol, BF Goodrich), anionic polymer compounds such as acrylic acid / alkyl methacrylate copolymer (Pemulene, BF Goodrich), and acetic acid amphoteric products of dialkylaminoethyl methacrylate polymers (Yukaformer, Mitsubishi Chemical) Amphoteric polymer compounds such as octyl acrylate acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, NSC), quaternized vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, ISP) , Methyl vinyl imidazolium chloride Cationic polymer compounds such as vinyl / vinylpyrrolidone copolymer (rubicoat, manufactured by BASF), polyvinylpyrrolidone (rubiscol K, manufactured by BASF), vinylpyrrolidone / vinyl acetate copolymer (rubiscol VA, manufactured by BASF) ), Nonionic polymer compounds such as vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer 937, manufactured by ISP), vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer VC713, manufactured by ISP), etc. There is. Cellulose or derivatives thereof, keratin and collagen or derivatives thereof, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharide, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, crystalline cellulose, arabino Naturally derived polymer compounds such as galactan, gum karaya, gum tragacanth, alginic acid, albumin, casein, curdlan, gellan gum and dextran can also be suitably used.
(9) Examples of physiologically active ingredients Examples of physiologically active ingredients include substances that impart some physiological activity to the skin when applied to the skin. For example, whitening ingredients, anti-inflammatory agents, anti-aging agents, UV protection agents, slimming agents, tanning agents, antioxidants, hair growth agents, hair restorers, moisturizers, blood circulation promoters, antibacterial agents, bactericides, desiccants, A cooling sensation agent, a warming sensation agent, vitamins, an amino acid, a wound healing promoter, an irritation relaxation agent, an analgesic agent, a cell activator, an enzyme component, etc. are mentioned. Examples of these suitable ingredients include, for example, ashitaba extract, avocado extract, amateur extract, altea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo biloba extract, fennel extract, turmeric extract, oolong tea extract, age Extract, Echinacea leaf extract, Ogon extract, Oat extract, Oen extract, Barley extract, Hypericum extract, Oyster extract, Dutch mustard extract, Orange extract, Seawater dried product, Seaweed extract, Hydrolyzed elastin, Hydrolyzed wheat powder, Hydrolyzed silk , Chamomile extract, carrot extract, cormorant extract, licorice extract, calcade extract, oyster extract, kiwi extract, kina extract, cucumber extract, guanosine, gardenia extract, kumazasae Kiss, Clara extract, Walnut extract, Grapefruit extract, Clematis extract, Chlorella extract, Mulberry extract, Gentian extract, Black tea extract, Yeast extract, Burdock extract, Rice bran ferment extract, Rice germ oil, Comfrey extract, Collagen, Cowberry extract, Saisin extract , Psycho extract, Saitai extract, Salvia extract, Soybean extract, Sasa extract, Hawthorn extract, Salamander extract, Shiitake extract, Giant extract, Shikon extract, Perilla extract, Linden extract, Shimotake extract, Japanese peony extract, Ginger root extract, Birch extract, Japanese cedar Extract, Kizuta extract, Hawthorn extract, Elderberry extract, Achillea millefolium extract, Atlantic mint extract, Sage extract, Zeni mallow Extract, cucumber extract, assembly extract, soybean extract, thymus extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, toki extract, toukisenka extract, tonin extract, spruce extract, dokudami extract, tomato extract, natto extract, carrot extract, Garlic extract, Novara extract, Hibiscus extract, Bacmond extract, Parsley extract, Honey, Camelis extract, Parietalia extract, Butterberry extract, Bisabolol, Biwa extract, Japanese dandelion extract, Fukinoto extract, Bukcho extract, Butcher bloom extract, Grape extract, Propolis, Loofah extract, safflower extract, peppermint extract, bodaiju extract, button extract, hop extract, pine extract, maronier extract Citrus Extract, Mulberry Extract, Melissa Extract, Peach Extract, Cornflower Extract, Eucalyptus Extract, Yukinoshita Extract, Yuzu Extract, Yakuinin Extract, Artemisia Extract, Lavender Extract, Apple Extract, Lettuce Extract, Lemon Extract, Forsythia Extract, Rose Extract, Rosemary Extract, Roman chamomile extract, royal jelly extract and the like.
Biopolymers such as deoxyribonucleic acid, mucopolysaccharide, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, hydrolyzed eggshell membranes, amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidonecarboxylate , Moisturizing ingredients such as betaine, whey, trimethylglycine, oily ingredients such as sphingolipid, ceramide, phytosphingosine, cholesterol, cholesterol derivatives, phospholipids, ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, Anti-inflammatory agents such as hydrocortisone, vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, vitamin C ester, etc. Active ingredients such as vitamins, allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as tocopherol, carotenoid, flavonoid, tannin, lignan, saponin, α-hydroxy acid, β-hydroxy acid, etc. Cell activators, blood circulation promoters such as γ-oryzanol and vitamin E derivatives, wound healing agents such as retinol and retinol derivatives, whitening such as arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid and glutathione Agent, cephalanthin, licorice extract, capsicum tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivative, calcium pantothenate, D-pant Tenenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol, ethinyl estradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, takanal, camphor, salicylic acid, nonyl acid vanillylamide, nonanoic acid vanillylamide, pyroctonola Mines, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone, cantalis tincture, cyclosporine, zinc pyrithione, hydrocortisone, minoxidil, polymonostearate Examples include hair restorers such as oxyethylene sorbitan, mint oil, and Sasanishiki extract.
(10) Examples of antioxidants Sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolyl biguanide, nordihydroguaiaretic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxy Examples include plant extracts having an antioxidant effect such as toluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoid, flavonoid, tannin, lignan, saponin, apple extract and clove extract.
(11) Examples of solvents Purified water, ethanol, lower alcohol, ethers, LPG, fluorocarbon, N-methylpyrrolidone, fluoroalcohol, volatile linear silicone, next-generation chlorofluorocarbon and the like.

  Examples of the present invention will be described below. In addition, the Example shown below is demonstrated for confirmation of the diet effect etc. of the composition obtained by this invention, and the scope of the present invention is not limited to these products and manufacturing methods.

[Manufacture of diet composition]
Indonesian Robusta coffee beans were used as the raw coffee beans. First, green coffee beans were pressed to separate the oil, and 1 kg of pressed product was obtained. 1 kg of this pressed product was crushed and refluxed with N-hexane, and the oil remaining in the pressed product was removed to obtain a defatted product. Next, this defatted product was extracted at 50 ° C. hydrous ethanol having an ethanol concentration of 60 wt%, and the ethanol extract was dried to obtain 60 g of fresh coffee bean extract (Example: composition for diet). The components contained in the green coffee bean extract (Example) were analyzed by HPLC, and found to be about 45 wt% of chlorogenic acids (including 20 wt% of chlorogenic acid) and about 10 wt% of caffeine.

[Diet effect confirmation test for weight gain and body fat accumulation (in vivo)]
Mice were allowed to freely ingest feeds mixed with raw coffee bean extract (Example) and its components (mixed according to the content of caffeine or chlorogenic acid), respectively, to provide a diet effect on weight gain and body fat accumulation confirmed.

  The test methods were as follows: mice (ddY, male, 6 weeks old), fresh coffee bean extract (0.5 and 1 wt%), caffeine (0.05 and 0.1 wt%), chlorogenic acid (0.15 and 0 Feed (CE-2, Nippon Claire) mixed with any one of. During this time, the body weight was measured every two days, and then the weight of the epididymis fat, which is one of the visceral fat, was measured on the final day. In addition, there was almost no difference in the amount of food consumed between the control group (bred with only feed) during breeding and each sample administration group. The results are shown in FIG. 1 and FIG.

  As shown in FIG. 1, the raw coffee bean extract (Example) showed a clear weight gain inhibitory effect, whereas chlorogenic acid and caffeine did not show a sufficient weight gain inhibitory effect. . This is thought to be due to the fact that specific components other than chlorogenic acid and caffeine contained in the raw coffee bean extract (Example) are involved in the suppression of weight gain.

  Moreover, as shown in FIG. 2, the raw coffee bean extract (Example) showed an accumulation inhibitory action on the weight of the testicular fat and the weight around the kidney. Chlorogenic acid and caffeine also showed a slight fat accumulation inhibitory effect.

  In FIG. 3, the graph which compared the anti-obesity effect with respect to the weight gain of a raw coffee bean extract (Example) and a roasted coffee bean extract (comparative example) was shown. In the test shown in FIG. 3, the roasted coffee bean extract is obtained by roasting raw coffee beans (undefatted) under normal conditions, and using the same extraction as the raw coffee bean extract (Example). Manufactured under conditions. Moreover, the weight change of the roasted coffee bean extract (comparative example) was measured up to the 5th day under the same conditions as the test of the green coffee bean extract (example) described above.

  As shown in FIG. 3, the raw coffee bean extract (Example) was found to have an effect of suppressing weight gain superior to the roasted coffee bean extract (Comparative Example). Thereby, it turns out that a green coffee bean extract (Example) has a high diet effect compared with a roasted coffee bean extract (comparative example).

  Next, the diet effect with respect to the weight gain at the time of combining a raw coffee bean extract (Example) and an existing diet material (Salacia extract, evening primrose extract, sesamin, garcinia) was confirmed. The Salacia extract is obtained by solvent extraction of the root of Salacia, and the evening primrose extract is obtained by solvent extraction of the evening primrose seed part.

In the test method, mice (ddY, male, 5 weeks old) were allowed to freely ingest a diet (CE-2, Nippon Claire) mixed with the sample shown in Table 1 for 4 days, and the weight gain was measured.
In addition, the mixed food amount of the sample was 0.5 wt% of fresh coffee bean extract, 0.5 wt% of Salacia extract, 3.0 wt% of evening primrose extract, 0.5 wt% of sesamin, and 1.0 wt% of Garcinia respectively.

  As shown in Table 1, when the raw coffee bean extract (Example) is used in combination with Salacia extract, evening primrose extract, sesamin or garcinia, the weight gain inhibitory effect is effectively enhanced as compared with the case of single administration. I understand that.

  Thus, the diet prescription by the raw coffee bean extract (Example) can enhance the diet effect by the combination of Salacia extract, evening primrose extract, sesamin or garcinia and the raw coffee bean extract (Example). That is, the raw coffee bean extract (Example) can obtain a more excellent diet effect when used in combination with at least one of Salacia extract, evening primrose extract, sesamin, and garcinia.

  Examples of other combinations include fresh coffee bean extract, Gymnema sylvestre, mulberry leaves, guava leaves, white kidney beans, glucomannan, yacon, chia seed, fenugreek, wheat amylase inhibitor, bittern, conjugated linoleic acid, L- Carnitine, Coleus forskohlii, Mate, Pepper, Citrus aurantium, Pepper, Capsiate, Cassia polyphenol, Mars extract, Green tea extract, Green tea polyphenol, Soy isoflavone, Black rice extract, Chitosan, Raspberry ketone, etc. Even when combined, an excellent diet effect can be obtained.

[Diet effect confirmation test in fat metabolism pathway]
(1) Confirmation of fat absorption inhibitory action a. Fat absorption delay action (in vivo):
A single dose of olive oil was administered to mice, and the effect of fresh coffee bean extract (Example) on absorption of ingested fat was confirmed.

  In the test method, blood was collected from a fasted (20 hours) mouse (ddY, male, 6 weeks old), and after 30 minutes, a 5 w / v% gum arabic suspension of raw coffee bean extract (Example) (10 mL / kg) was orally administered. Olive oil (5 mL / kg) was orally administered 1 hour later, and blood was collected at 2, 4 and 6 hours thereafter. Serum was separated from the obtained blood, and the triglyceride concentration was measured using an enzymatic method (Triglyceride E-Test Wako, manufactured by Wako Pure Chemical Industries, Ltd.).

  As shown in FIG. 4, the fresh coffee bean extract (Example) showed significant suppression of blood triglyceride elevation compared to the control group, and the fresh coffee bean extract has a strong fat absorption inhibitory action. It was confirmed that there was.

b. Pancreatic lipase inhibitory activity (in vitro):
About the raw coffee bean extract (Example) and its components (caffeine and chlorogenic acid), the inhibitory activity against pancreatic lipase involved in the degradation of ingested fat was evaluated in vitro.
The inhibitory activity of pancreatic lipase was measured using porcine pancreatic lipase (manufactured by SIGMA, final concentration 105.8 units / mL) and lipase kit-S (Dainippon Pharmaceutical). The results are shown in FIG.

  As shown in FIG. 5, green coffee bean extract (Example), chlorogenic acid and caffeine showed pancreatic lipase inhibitory activity and were confirmed to suppress fat absorption. In addition, the green coffee bean extract (Example) showed pancreatic lipase inhibitory activity superior to chlorogenic acid and caffeine. Considering the content of chlorogenic acid (about 20 wt%) and caffeine (about 10 wt%) in the raw coffee bean extract (Example), the pancreatic lipase inhibitory activity of such raw coffee bean extract (Example) is It is considered that components other than chlorogenic acid and caffeine are involved in pancreatic lipase inhibition.

(2) Fat accumulation inhibitory action confirmation test a. 3T3-L1 adipocyte differentiation inhibitory action (in vitro):
The raw coffee bean extract (Example) and its components (caffeine and chlorogenic acid) were added to the mouse adipocyte cell line (3T3-L1) culture system, and the effect on fat accumulation after induction of differentiation was confirmed.

In the test method, 3T3-L1 adipocytes (5 × 10 ⁴ cells / mL) were cultured in DMEM medium (high glucose) containing 10 wt% fetal calf serum for 2 days, then insulin (1 μg / mL), dexamethasone (0. 25 μM) and isobutylmethylxanthine (0.5 mM) were exchanged to induce differentiation. Two days later, the medium was replaced with a medium containing sample and insulin (1 μg / mL), and cultured for a total of 6 days while changing the medium every other day. After completion of the culture, the triglyceride concentration in the cells and the glycerol 3-phosphate dehydrogenase (GPDH) activity, which is an index of adipocyte differentiation, were measured.

As shown in FIG. 6, mild accumulation inhibition (reduction of accumulated triglycerides) was observed in raw coffee bean extract (Example), caffeine and chlorogenic acid.
Moreover, about the glycerol 3-phosphate dehydrogenase activity (GPDH activity) used as the parameter | index of adipocyte differentiation, the slight fall of raw coffee bean extract (Example), caffeine, and chlorogenic acid was recognized. No cytotoxicity was observed at the same concentration.

b. Fatty liver inhibitory action (in vivo):
The effects of raw coffee bean extract and components (caffeine and chlorogenic acid) on liver lipids (triglycerides and total cholesterol) when administered continuously (2 weeks) to mice were evaluated.

  In the test method, mice (ddY, male, 5 weeks old) were preliminarily raised for 1 week, divided into 4 groups, and a sample suspended in 5% w / v gum arabic once a day was orally administered for 2 weeks. On the final day of the experiment, the liver was removed under non-fasting conditions, and the liver triglyceride and total cholesterol contents were measured using a kit manufactured by Wako Pure Chemical Industries. The results are shown in FIG.

  As shown in FIG. 7, raw coffee bean extract (Example), caffeine and chlorogenic acid decreased liver triglycerides, and it was confirmed that caffeine and chlorogenic acid were particularly effective. On the other hand, the total cholesterol level was almost the same as that of the control. From these results, the raw coffee bean extract has an action of suppressing fatty liver due to accumulation of neutral fat, which suggests the involvement of caffeine and chlorogenic acid.

(3) Confirmation test for promoting lipolysis a. Lipolysis (in vitro):
It is known that caffeine has an action of activating lipase in adipocytes and promoting the degradation of neutral fat. In this test, the lipolytic action of fresh coffee bean extract and its components (caffeine and chlorogenic acid) was compared with existing diet materials.

  In the test method, the epididymis fat was extracted from Wistar male rats and incubated (37 ° C., 3 hours) in various samples dissolved in Medium 199 (medium). After completion of the incubation, fat was removed and the amount of glycerol in the medium was measured using F-kit glycerol (manufactured by Nippon Roche). The results are shown in FIG.

  As shown in FIG. 8, the lipolytic action when 1000 μg / mL of fresh coffee bean extract (Example) is added is equivalent to caffeine, capsaicin and synephrine as single compounds, and contains about 30 wt% of synephrine. It turned out to be stronger than citrus extract. Chlorogenic acid was also found to have a mild lipolytic action.

b. Blood triglyceride lowering effect (in vivo):
The effect of fresh coffee bean extract and its components (caffeine and chlorogenic acid) on blood triglycerides when orally administered to fasting mice was evaluated.

  In the test method, blood was collected from a fasted (24 hours) mouse (ddy, male, 6 weeks old) vein, and various samples (10 mL / kg) suspended in 5 w / v gum arabic 30 minutes later. Mice were orally administered. Thereafter, blood was collected over time every hour, and blood triglycerides were measured. As a control, blood triglyceride was also measured under the same conditions for the oral administration of gum arabic alone.

As shown in FIG. 9, the raw coffee bean extract (Example) showed a strong blood triglyceride lowering action similar to capsaicin. Caffeine also showed a strong blood triglyceride lowering effect. On the other hand, chlorogenic acid showed a blood triglyceride lowering effect equivalent to synephrine.
From these test results, the raw coffee bean extract (Example) has an action of promoting lipolysis in adipocytes, and it is suggested that caffeine and chlorogenic acid are involved in the action.

(4) Confirmation of fat burning promotion action (carnitine palmitoyltransferase (CPT) activity promotion test: in vivo)
Mice were fed with a feed containing raw coffee bean extract (Example), and carnitine palmitoyltransferase (CPT) activity contributing to β-oxidation of fat burning metabolism was measured.

  In the test method, mice (ddY, male, 7 weeks old) were allowed to freely ingest a diet (CE-2, Nippon Claire) mixed with fresh coffee bean extract (0.5 and 1 wt%) for 6 days. After dislocation of the cervical vertebrae, the liver was removed, homogenized with 6 times the amount of 0.25M sucrose and 1 mM EDTA-containing Tris buffer (pH 7.4), and centrifuged (3000 rpm, 10 minutes). It was. The supernatant was centrifuged again (11000 rpm, 10 minutes), and the resulting precipitate (mitochondrial fraction) was suspended by adding a buffer solution (2.5 mL). After protein quantification, CPT activity was measured by the DTNB method. The results are shown in FIG.

  As shown in FIG. 10, the green coffee bean extract (Example) showed a dose-dependent CPT activity promoting effect. From this result, it was confirmed that the green coffee bean extract (Example) promotes CPT activity and assists fat burning.

[Diabetes prevention confirmation test]
a. Glucose absorption delaying action (in vivo):
The inhibitory effect on the increase in blood glucose level at the time of carbohydrate administration of fresh coffee bean extract was confirmed using a mouse carbohydrate loading model.

  In the test method, blood was collected from fasted (18 hours) mice (ddy, male, 6 weeks old), and immediately thereafter, an aqueous solution (10 mL / kg) of fresh coffee bean extract (Example) was orally administered. One hour later, glucose (0.5 g / kg) or sucrose (2 g / kg) was orally administered (5 mL / kg), and blood was collected at 0.5, 1 and 2 hours thereafter. Serum was separated from the obtained blood, and the glucose concentration was measured by an enzymatic method (Determiner GL-E: manufactured by Kyowa Medex).

  As shown in FIG. 12, it was confirmed that the raw coffee bean extract (Example) suppressed the increase in blood glucose level by administration of 400 mg / kg when loaded with glucose and administration of 200 mg / kg when loaded with sucrose.

b. α-Glucosidase inhibitory activity (in vivo):
The raw coffee bean extract and its components (chlorogenic acid, caffeic acid, caffeine and quinic acid) were tested for inhibitory activity against α-glucosidase, which is a carbohydrase.

In the test method, about 10-fold amount of 0.1 M phosphate buffer (pH 7.0) was added to rat small intestine acetone powder (Sigma), and the centrifuged supernatant was used as an enzyme solution. 0.2 mM 4-methylumbelliferyl-α-D-glucopyranoside (Sigma) was used as a substrate. Each sample was dissolved in DMSO, and a 2-fold dilution series was prepared with a phosphate buffer containing 4% DMSO. Add sample diluent (50 μL / well) and substrate buffer (25 μL / well) to the microplate, preheat (37 ° C., 10 minutes), then add enzyme solution (25 μL / well) at 37 ° C. The reaction was performed for 30 minutes (final enzyme concentration: 1 mg protein / mL, final substrate concentration: 0.05 mM). 0.2 M Na ₂ CO ₃ (100 μL / well) was added to stop the reaction, and fluorescence intensity (excitation wavelength: 366 nm, measurement wavelength: 450 nm) was measured using a microplate reader.

  As shown in Table 2, raw coffee bean extract (Example), chlorogenic acid and caffeic acid showed strong inhibitory activity against α-glucosidase, whereas caffeine and quinic acid showed inhibitory activity. I couldn't. Moreover, the inhibitory activity of the green coffee bean extract (Example) was superior to that of chlorogenic acid and caffeic acid.

[Composition example]
The compounding example of the composition for diet by this invention is shown. In the following formulation examples, a water-containing ethanol extract of defatted raw coffee beans can be used as the “raw coffee bean extract”. Each formulation example includes the second invention (a composition for inhibiting fat absorption), the third invention (a composition for inhibiting pancreatic lipase activity), the fourth invention (a composition for promoting carnitine palmitoyltransferase activity) and the fifth invention (α- The same applies to the composition for inhibiting glucosidase activity).

As described above, according to the present invention, the following excellent effects can be obtained.
(a) By taking a safe extract derived from green coffee beans, an excellent diet effect can be obtained, and lifestyle-related diseases such as diabetes can be effectively prevented or treated.
(b) Since it is a safe extract derived from green coffee beans, it can be used with peace of mind as a raw material for food and drinks and medicines.

It is a graph which shows the effect | action which acts on the weight gain of a mouse | mouth at the time of continuous intake of a raw coffee bean extract and its component (caffeine and chlorogenic acid). It is a graph which shows the effect | action which acts on a mouse | mouth visceral fat at the time of continuous intake of a raw coffee bean extract and its component (caffeine and chlorogenic acid). It is a graph which shows the effect | action which acts on the mouse | mouth weight increase at the time of continuous intake of a raw coffee bean extract and a roasted coffee bean extract. It is a graph which shows the effect | action which acts on the blood triglyceride at the time of olive oil loading of a raw coffee bean extract. It is a graph which shows pancreatic origin lipase inhibitory activity of a raw coffee bean extract and its component (caffeine and chlorogenic acid). It is a graph which shows the effect | action which acts on 3T3-L1 adipocyte differentiation of a raw coffee bean extract and its component (caffeine and chlorogenic acid). It is a graph which shows the effect | action which acts on a mouse | mouth liver lipid at the time of continuing administration of the raw coffee bean extract) and its component (caffeine and chlorogenic acid). It is a graph which shows the effect | action which acts on the glycerol release from the testicular fat of raw coffee bean extract, its containing component (caffeine and chlorogenic acid), and a lipolysis material. It is a graph which shows the effect | action which acts on the triglyceride in mouse | mouth blood of a raw coffee bean extract, its containing component (caffeine and chlorogenic acid), and the existing diet raw material. It is a graph which shows the effect | action which acts on the liver mitochondrial fraction CPT activity of a raw coffee bean extract. It is a schematic diagram for demonstrating the diet effect in a fat metabolic pathway. It is a graph which shows the blood glucose level raise inhibitory effect at the time of glucose and sucrose loading of a raw coffee bean extract.

Claims (29)

  A diet composition comprising a polar solvent extract of defatted green coffee beans as an active ingredient.   The composition for diet according to claim 1, wherein the polar solvent extract is a hydrous ethanol extract.   The composition for diet according to claim 1, wherein the polar solvent extract is a hydrous ethanol extract having an ethanol concentration of 40 to 90% (wt / wt).   The composition for diet according to claim 1, 2, or 3, wherein the defatted raw coffee beans are obtained by extracting and separating the oil content of the raw coffee beans with N-hexane.   A composition for diet, comprising at least one of Salacia extract, evening primrose extract, sesamin and garcinia added to the diet composition according to claim 1.   Food / beverage products containing the composition for diets as described in any one of Claims 1-5 as an active ingredient of a diet effect | action.   The chemical | medical agent containing the composition for diets as described in any one of Claims 1-5 as an active ingredient of a diet effect | action.   The skin external preparation containing the composition for diets as described in any one of Claims 1-5 as an active ingredient of a diet effect | action.   A composition for suppressing fat absorption, comprising a raw coffee bean extract as an active ingredient.   The composition for fat absorption suppression according to claim 9, wherein the raw coffee bean extract is a polar solvent extract of defatted raw coffee beans.   The composition for fat absorption suppression according to claim 10, wherein the polar solvent extract is a hydrous ethanol extract.   The composition for fat absorption suppression according to claim 10, wherein the polar solvent extract is a hydrous ethanol extract having an ethanol concentration of 40 to 90% (wt / wt).   The composition for fat absorption suppression according to claim 10, 11 or 12, wherein the defatted raw coffee beans are obtained by extracting and separating the oil content of the raw coffee beans with N-hexane.   Food / beverage products containing the composition for fat absorption suppression as described in any one of Claims 9-13 as an active ingredient of a fat absorption suppression effect.   The chemical | medical agent containing the composition for fat absorption suppression as described in any one of Claims 9-13 as an active ingredient of a fat absorption suppression effect.   A composition for inhibiting pancreatic lipase activity, comprising a raw coffee bean extract as an active ingredient.   The composition for inhibiting pancreatic lipase activity according to claim 16, wherein the raw coffee bean extract is a polar solvent extract of defatted raw coffee beans.   The composition for inhibiting pancreatic lipase activity according to claim 17, wherein the polar solvent extract is a hydrous ethanol extract.   The composition for inhibiting pancreatic lipase activity according to claim 17, wherein the polar solvent extract is a hydrous ethanol extract having an ethanol concentration of 40 to 90% (wt / wt).   20. The composition for inhibiting pancreatic lipase activity according to claim 17, 18 or 19, wherein the defatted raw coffee beans are obtained by extracting and separating the oil content of raw coffee beans with N-hexane.   Food / beverage products containing the composition for pancreatic lipase activity inhibition as described in any one of Claims 16-20 as an active ingredient of pancreatic lipase activity inhibitory action.   The chemical | medical agent containing the composition for pancreatic lipase activity inhibition as described in any one of Claims 16-20 as an active ingredient of a pancreatic lipase activity inhibitory effect.   The composition for alpha-glucosidase activity inhibition containing a raw coffee bean extract as an active ingredient.   The composition for inhibiting α-glucosidase activity according to claim 23, wherein the raw coffee bean extract is a polar solvent extract of defatted raw coffee beans.   The composition for inhibiting α-glucosidase activity according to claim 24, wherein the polar solvent extract is a hydrous ethanol extract.   The composition for inhibiting α-glucosidase activity according to claim 24, wherein the polar solvent extract is a hydrous ethanol extract having an ethanol concentration of 40 to 90% (wt / wt).   27. The composition for inhibiting α-glucosidase activity according to claim 24, 25 or 26, wherein the defatted raw coffee beans are obtained by extracting and separating the oil content of raw coffee beans with N-hexane.   The food-drinks containing the composition for alpha-glucosidase activity inhibition as described in any one of Claims 23-27 as an active ingredient of alpha-glucosidase activity inhibition action. The chemical | medical agent containing the composition for alpha-glucosidase activity inhibition as described in any one of Claims 23-27 as an active ingredient of alpha-glucosidase activity inhibition action.

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