JP2013537528A - Use of a mixture of roasted and non-roasted coffee beans to control skin pigmentation - Google Patents

Abstract

The present invention relates generally to the field of food supplements and / or food products intended for beauty. More particularly, the present invention prevents the skin from hyperpigmentation, skin imperfections such as stains, and other skin disorders characterized by abnormal pigments, containing a mixture of roasted and non-roasted coffee beans and It aims at providing the raw material for / or treating. Furthermore, in addition to providing a skin lightening agent or whitening agent, the present invention aims to improve the color tone of the skin.
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Description

Field of the Invention

  The present invention relates generally to the field of food supplements and / or food products intended for beauty. More particularly, the present invention prevents and / or prevents skin hyperpigmentation, skin imperfections such as stains, and other skin disorders characterized by abnormal pigments, comprising a mixture of roasted and non-roasted coffee beans. Or it aims at providing the raw material for treating. In addition to providing skin lightening or whitening agents, the present invention is further directed to improving the color of the skin.

Background of the invention

  Skin color is mainly determined by the amount and type of brown pigment present in the skin, melanin. The skin color becomes brighter as the amount of melanin is smaller and darker as it is larger. Also, skin hyperpigmentation is caused by over expression or accumulation of melanin in the skin. Therefore, the pathways involved in melanin production have been targeted by many inhibitors in order to reduce production levels. One of the major enzymes involved in the melanin pathway is tyrosinase.

  The synthesis of melanin is a process controlled by hormones including melanocyte stimulating hormone (MSH) and adrenocorticotropic hormone (ACTH) peptide produced from the precursor proopiomelanocortin. This process is stimulated by DNA damage caused by UVB radiation.

  Thus, prolonged exposure to sunlight can trigger many biochemical reactions in the skin, leading to, for example, sunburn and darkening. Other effects of exposure to sunlight accumulate over time. Such changes may cause the development of stains, which may result in uneven and mottled skin tone. Unfortunately, many of the commercial products on the market today are either less effective or contain an active agent that is unstable and loses efficacy when incorporated into the final formulation.

  The ability to modify the expression of pigment content in the skin, to promote evenness of skin tone, or to promote lightening of skin tone, is strongly desired in today's society. For purely cosmetic reasons, many people want to change the color of the skin, reduce the stains, etc.

  Therefore, efforts to develop effective compositions have focused on agents that inhibit the activity of tyrosinase. For example, various tyrosinase inhibitors have been proposed for use in topical compositions such as hydroquinone, vitamin C, cysteine, kojic acid, arbutin, glutathione, among others. In addition, various skin compositions have been proposed for ameliorating the occurrence of pigment abnormalities observed in melasma, sputum egg spots, vitiligo, mottle syndrome, phenylketonuria and the like, and / or for cosmetic purposes. The

  Furthermore, the use of skin bleaching compositions is also expanding. However, such compositions are those that destroy melanin or inhibit the production of melanin. Many such compositions contain strong chemicals such as peroxides, acids or formaldehyde or thiolated substances. Even less rigorous therapies have other drawbacks.

  Just as laser therapy and chemical peel have been proposed, topical retinoids and topical corticosteroids have been proposed as depigmenting agents, but the desired response may not always be obtained.

  The use of other compositions of natural material on the skin has also been proposed. Some of them have been used for centuries in Asia and Europe to decolorize the skin and skin area and to improve the appearance of fair skin. The use of lemon, orange, cucumber, ginkgo biloba, carob, Agetsu, Ginseng, cinnamon, mayorana, rosemary and the like is exemplified.

  When applied topically to the skin to combat diseases associated with pigment abnormalities or to lighten the color of the skin, various compounds that can reduce tyrosinase activity and consequently reduce melanin production It has been proposed. Unfortunately, currently available treatments are not always satisfactory, particularly with regard to the side effects associated with many treatments, such as the irritant side effects associated with the use of certain topical agents.

  Therefore, it is highly desirable to obtain alternative preparations which do not suffer from the disadvantages of the prior art described above. In particular, the development of nutritional cosmetic compositions that are administered via an oral route with improved stability, promoting uniformity of skin color, or lightening the color of skin is strongly desired. It is desired.

  There also remains a need for active agents that are effective for the treatment and / or prevention of skin pigmentation disorders, in particular skin pigmentation disorders due to environmental factors or aging.

  The object of the present invention is to meet these needs.

  The present inventors can achieve this object by providing a food or food supplement composition comprising at least one ingredient comprising a mixture of roasted and non-roasted coffee beans.

  Thus, according to the first subject, the present invention relates to an active agent for the treatment and / or prevention of skin pigmentation, of at least one raw material containing portions of roasted and non-roasted coffee Use for the purpose of an effective amount of beauty. Such skin disorders are in particular due to aging such as stains or environmental factors (eg UV). It may be a skin disease observed in melasma, sputum egg spots, vitiligo, mottleflies, phenylketonuria.

  The inventors have found that a mixture of non-roasted and roasted coffee beans effectively suppresses the formation of melanin, i.e. melanogenesis, and the extract has little or no inhibition of tyrosinase activity. This result is surprising and unexpected given the important role of tyrosinase in melanogenesis and the development effort to inhibit this enzyme in the field.

  For the purposes of the present invention, the term "skin" is intended for use in the sense of the skin of the face or body.

  For the purposes of the present invention, the term "effective amount" is intended to be used in the sense of an amount sufficient to obtain the expected effect.

  For the purpose of the present invention, the term "preventing" is intended for use in the sense of reducing the risk of developing the symptoms of the disease in question.

  The invention further relates to the cosmetic use of the above-mentioned raw materials as an active agent for the treatment and / or prevention of skin pigmentation defects. The result is a brighter, more uniform complexion without abnormal skin areas or dry areas.

  Furthermore, the present invention is a cosmetic use of an effective amount of at least one raw material containing a portion of non-roasted and roasted coffee according to the invention, wherein the skin color is whitened or lightened. Use as an active agent to

  The inventors have also found that the raw materials according to the invention further improve the hydration and / or skin barrier function.

  The use according to the invention comprises at least one of at least one raw material containing portions of non-roasted and roasted coffee, in particular for further improving skin hydration or skin aging, as described below. It may further comprise a combination with an effective amount of a type of active agent.

  According to another aspect, the subject matter of the present invention provides, in particular, a cosmetic for treating and / or preventing skin mispigmentation and diseases associated with hyperpigmentation in an individual, in particular aesthetic disorders. A method of interest comprising at least one step of administering to said individual at least one ingredient containing portions of non-roasted and roasted coffee according to the present invention.

  The compositions according to the invention are orally administrable. This has the advantage of being able to act broadly over the entire skin, ie deep layers (dermis, subcutaneous tissue) by means of a rapid and relatively limited method of administration. Specifically, metabolites and other active nutrients can be dispersed in the skin matrix, in particular by the bloodstream. Oral administration also has the advantage of being a rapid and relatively unrestricted method of administration.

Detailed Description of the Invention

  According to a first object, the present invention provides the use of a coffee product according to the method of blending coffee beans, which are partially roasted beans and partly non-roasted or non-roasted beans.

  In the following description, "coffee beans" are obtained by the wet method (fermentation, washing) or the dry method (mechanical peeling after drying) starting from coffee "cherry" and after such peeling It must be understood to mean beans.

  “Extract” is to be understood as meaning any compound obtained starting from an alcohol or alcohol aqueous solution extraction of crude product, in this case roasted or non-roasted decaffeinated coffee beans. You must. The species of coffee tree selected for the preparation of the coffee bean extract used in the composition is advantageously selected from the genus Coffee.

  The coffee tree is a shrub with perennial, leathery shiny leaves (10-15 × 4-6 cm) with smooth leaf edges. A lot of white aromatic flowers are put in the ring body in the axilla. In fact, it is a green thorn, which turns red upon ripening, and usually contains two hemispherical seeds with flat sides facing each other. Although only two species (Arabica and Canephora species) meet the demand for the coffee market, there are many wild species of coffee in tropical forests of East Africa.

  Coffee "beans" are obtained by coffee "cherries", ie wet processes (fermentation, washing) or dry processes (mechanical peeling after drying), starting from the kernel. The pulp is removed by pulping the red skin and the fleshy mesocarp to reach the "shell" of the coffee. Exfoliation (removal of woody endocarp) results in coffee "seeds" (or beans).

  In certain embodiments, the non-roasted coffee portion may be a blend of non-roasted Arabica beans and non-roasted Robusta beans. However, it is particularly preferred that the non-roasted coffee portion is derived from most or all of the robusta coffee beans.

  Thus, preferably at least 65%, more preferably 75%, even more preferably 85%, most preferably 95%, for example 100% by weight of the non-roasted coffee beans are lobsta seeds.

  The roasted coffee portion may be a blend of arabica and lobsta species, but it is particularly preferred that the roasted portion be most or all derived from arabica bean. This is because the Arabica species has a richer taste and aroma associated with higher quality coffee.

  The roasting level of the roasted coffee may vary from light to deep roasting, but CTN 60-100 is preferred.

  The non-roasted coffee portion and / or the roasted coffee portion may be at least partially devoid of caffeine. This is to provide a product that can adjust caffeine levels from no caffeine to normal caffeine levels. However, it is particularly preferable that the non-roasted coffee part is derived from decaffeinated coffee.

  In particular, the coffee bean extract can be obtained by aqueous alcohol extraction or alcohol extraction of coffee beans, preferably with methanol, ethanol or propanol. It is preferred that the coffee bean extract does not contain a fraction of coffee beans extractable by the nonpolar solvent. Methods of preparing decaffeinated coffee extracts are well known in the art.

  In one embodiment of the present invention, the coffee product is a mixture of non-roasted coffee beans and roasted coffee beans, wherein the weight ratio of non-roasted part to roasted part is 1:99 to 90:10, preferably From a mixture of 30:70 to 50:50, more preferably 35:65 to 45:55, most preferably 37:63 to 42:58. Such coffee products are described in EP 1871173, which is incorporated herein by reference.

In particular, coffee products are preferably
(I) 1 to 90% by weight, based on the total weight of the coffee product, of the first portion consisting of unroasted ground coffee and / or unground coffee,
(Ii) comprising 99 to 10% by weight of a second portion consisting of ground coffee having a higher degree of roasting than the first portion, relative to the total weight of the coffee product,
It comprises at least 2 g, more preferably at least 4 g, more preferably at least 6 g, most preferably at least 8 g, for example at least 9 g of chlorogenic acid per 100 g of coffee product.

  The composition according to the invention may be in the form of any galenic pharmaceutical normally used for the chosen method of administration. The carrier may have different properties depending on the type of composition targeted.

  The composition may be any food or pharmaceutical product for oral application or a product intended for beauty. Examples of food carriers or pharmaceutical carriers are nutritionally complete formulations, milk, yogurt, cheese, fermented milk, milk-based fermented products, dairy products such as ice cream, cereal-based products or fermented cereal-based products, Milk-based powders, infant and baby formula, refrigerated or cold-stable beverages, confectionery, chocolate or cereal type food products, animals, especially livestock feed. In particular, chicory or chicory extract may be incorporated into any other form of food supplement or fortified food, such as stick food or concentrated or non-concentrated powder. The powder may be diluted with water, carbonated water, milk products or soy derivatives and may be incorporated into stick food.

  The food product may further comprise protein source, carbohydrate source, lipid source, mineral source and / or vitamin source. The presence of proteins, carbohydrates, lipids, minerals and / or vitamins has several advantages. These compounds generally contribute to the taste and mouthfeel of the final product. In addition, these compounds provide the body with nutrients that the body urgently needs when suffering from a skin disease. Furthermore, these compounds make it possible to formulate the products of the invention as nutritionally complete formulations which do not require the addition of nutrients.

  Each raw material may contain any type of edible compound. Typical ingredients for food compositions, in particular beverages, are well known in the art, for example milk, cream, coffee whitener and coffee creamer. Alternatively, the raw material can be, for example, a salad dressing or a part thereof. Such compounds are used, for example, by consumers to modify the flavor, appearance and texture of the composition. The raw material may also be in liquid form or in dry form, eg a powder to be dissolved and / or suspended in the beverage.

  The invention relates to a coffee beverage product comprising as a raw material a coffee product as defined herein, such as (a) roast and ground coffee products, (b) soluble coffee products, (c) single dose coffee pads, tablets or capsules, Further provide (d) ready-to-drink coffee products or (e) any other suitable coffee products.

  Soluble coffee, also known as instant coffee, is widely known and has been produced commercially for many decades. Soluble coffee is produced in various ways from roast and roast coffee, all of which are well known to those skilled in the art.

  The invention is particularly suitable for use as a soluble coffee product or any product based on a soluble coffee product or any product derived from a soluble coffee product. For example, coffee products may also be used for so-called ready-to-drink beverages. Examples of ready-to-drink beverages suitable for the products of the invention include two-component beverages comprising a coffee component together with a natural or artificial sweetener component. These components may optionally be pre-diluted with a liquid such as water or milk. In this case, the coffee component may comprise the coffee product of the present invention.

  A three-component beverage comprising coffee, sweeteners and whiteners, such as milk, liquid creamers or solid (e.g. powder) creamers may comprise the coffee product of the present invention.

  Furthermore, any beverage containing soluble coffee as a raw material may comprise the coffee product of the present invention.

  The composition may further comprise additional ingredients suitable for use in the food composition. Common raw materials are, for example, sugars, artificial sweeteners, emulsifiers, stabilizers, thickeners, thickeners, dyes, flavors, flavors and the like. Suitable artificial sweeteners include saccharin, cyclo, acetosulfame, L-aspartyl based sweeteners such as aspartame, and mixtures thereof. Suitable emulsifying agents include monoglycerides, diglycerides, lecithin, diacetyl tartaric acid esters of monodiglycerides, starches for emulsifying, and mixtures thereof. Suitable stabilizers include dipotassium phosphate and sodium citrate. A suitable fluxing agent is sodium aluminosilicate. In one embodiment, the composition comprises milk protein and / or vegetable protein. In a further embodiment, the composition comprises milk fat and / or vegetable fat.

  In addition, the present invention can be used as a raw material for coffee, espresso, coffee-based beverage preparations, usually extracted for mass use or for single-dose beverage preparation at home.

  If the product is a nutritional supplement for oral administration, the product may be placed in capsules, gelatin capsules, soft capsules, tablets, sugar coated tablets, tablets, pastes or lozenges, gums, or drinking solutions or emulsions, syrups or gels. Such supplements may further comprise sweeteners, stabilizers, antioxidants, additives, flavoring agents and / or coloring agents. These formulations are carried out by the usual methods of preparing sugar coated tablets, gel capsules, gels, controlled release hydrogels, emulsions, tablets or capsules.

  In this regard, oral compositions, in particular adjuncts for nutraceuticals, are well known to the expert. Above all, purely for the purpose of illustration, lubricants such as magnesium stearate which are usually used in foodstuffs, products for instant solubilization, gelling agents, thickeners, humectants, fatty and / or aqueous Included are compounds, preservatives, texture modifiers and flavors and / or coatings, antioxidants and coloring substances.

  The composition according to the invention may additionally contain lipids, polyphenols, taurine, probiotic microorganisms, vitamins and / or oligo elements. When using probiotics, the probiotics may be included in the form of viable bacteria, and may be included in semi-activated or non-activated form, for example in the form of a lyophilized powder.

  Microbial culture supernatant may also be included in the composition. The microorganism may be selected from the group consisting of lactic acid bacteria, in particular Lactobacilli and / or Bifidobacterium, more preferably Lactobacillus johnsonii, Lactobacillus reuteri (Lactobacillus johnsonii). Lactobacillus reuteri), Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus casei, Bifidobacterium bifidum, Bifidobacterium breve B. dovebacterium breve), Bifidobacterium animalis (Bifidobacterium animais), Bifidobacterium infantis (Bifidobacterium infantis), Bifidobacterium dolescentis and Bifidobacterium pseudocatenulatum (Bifidobacterium) It is selected from the group consisting of pseudocatenulatum). According to the most preferred embodiment, the strain used is Lactobacillus johnsonii (Lal), deposited on June 30, 1992 at the Pasteur Institute according to the Budapest Treaty, and assigned the deposit number CNCM 1-1225, or Lactobacillus paracasei (ST11) deposited on Jan. 12, 1999 at the Institute of Pasteur in accordance with the Budapest Treaty, and assigned the deposit number CNCM I-2116. For example, the following compounds may be used alone or in combination. Zinc and zinc salts such as zinc sulfate and zinc gluconate, vitamin B5, B6, B8, C, E or PP, p-carotene and carotenoids, garlic extract, in particular in the form of allyl sulfide or garlic oil, selenium, curcumin , Curcuminoids, niacin, lithospermic acid and adenosine. As a matter of course, experts can improve the expected effect of the composition object of the invention by preventing such active compounds from inhibiting or attenuating the desired activity of interest. Choose and, if possible, combine.

Use The product of the present invention is used efficiently, for example, to treat or prevent dermatosis, by reducing the production of melanin, or to lighten the skin tone for cosmetic purposes. can do. It has been shown in practice that roasted and non-roasted (non-roasted) coffee bean blends reduce the synthesis of melanin in vitro (Example 1, Figure 1). The production of tyrosinase is also limited but decreased (Figure 2), suggesting that the reduction of melanin is not due to tyrosinase inhibition but by a mechanism acting upstream or downstream of this enzyme .

  The raw material according to the present invention strengthens the skin barrier and exerts a further positive effect on maintaining skin hydration.

  As a result, poor pigmentation is reduced, resulting in a brighter, more uniform complexion without abnormal skin areas or dry areas.

  Thus, according to one subject, the present invention comprises roasted and non-roasted coffee beans, in particular as active agents for the treatment and / or prevention of skin pigmentations due to environmental factors such as aging or UV. For the cosmetic use of an effective amount of at least one ingredient.

  The present invention is also particularly desirable for Asians, the effectiveness of at least one raw material containing roasted and non-roasted coffee beans as an active agent for whitening or lightening skin color. Regarding the use for the purpose of beauty of the quantity.

  The use according to the invention consists of at least one raw material containing roasted and non-roasted coffee beans, in particular at least one for improving skin hydration or skin aging as described below. It may further comprise a combination with an effective amount of an active agent.

  According to another aspect, the subject matter of the present invention is particularly aimed at the cosmetic treatment and / or prevention of skin mispigmentation and / or diseases associated with hyperpigmentation in an individual, in particular cosmetic diseases. The method according to the present invention comprises at least one step of administering to said individual at least one ingredient containing roasted and non-roasted coffee beans according to the present invention.

  The cosmetic method of treatment according to the invention can in particular be carried out by orally administering at least an effective amount of at least one ingredient containing roasted and non-roasted coffee beans according to the invention. Oral administration includes taking the oral composition as described above one or more times.

  Oral administration may involve a single application. According to another embodiment, the application is repeated for a period of time, for example from 1 to 2 times a day, to one or more days, generally at least 4 weeks, and sometimes from 1 to 15 weeks.

  In addition, combinations of therapeutic and oral or topical dosage forms may be envisioned as needed to supplement or enhance the activity of the raw materials as defined in the present invention.

  Therefore, orally or orally containing other active raw materials, in particular, probiotic microorganisms, or dead bacteria, live bacteria or other activated forms or other probiotics, or wettable powders or anti-aging agents as required Topical or oral treatment with a composition comprising roasted and non-roasted coffee beans according to the invention in combination with a composition for use can be envisaged as a kit. The ingredients are mixed prior to formulation according to the order and conditions readily determined by one skilled in the art.

  The ingredients are mixed prior to formulation according to the order and conditions readily determined by one skilled in the art.

  The proportion of coffee bean extract in the composition is of course determined according to the desired effect and the mode of administration of the composition.

  The daily dose of coffee bean extract administered by the oral route is preferably 0.01-5000 mg / day, depending on the caffeine content. The coffee bean blend is selected to be present in the composition according to the invention in an amount such that the dose is 0.5-1000 mg / day. We have found that the efficacy of the coffee bean blend according to the invention is generally dose dependent and follows a dose response curve. In general, when the product is used frequently to prevent mild skin disease or damage, only small amounts can provide the desired effect. When treating severe skin pigment abnormalities, even small amounts can be effective, but large amounts are more suitable.

  Preferably, the composition intended for oral administration is 10% to 100% by weight of the composition, preferably a composition of coffee bean extract depending on the product form (soluble coffee, RTD, coffee mix, food product or food supplement). It contains in 20 to 80 weight% of a thing.

  Further advantages and features of the present invention will be apparent from the following examples and figures. Accordingly, the following examples are given for the purpose of non-limiting illustration in the field of the present invention. In these embodiments, unless stated otherwise, the percentages are weight percentages, and the range of values shown in the form "...-..." includes the stated upper and lower limits.

Evaluation of melanin production by mouse melanocytes pretreated with coffee blend. Evaluation of tyrosinase production by mouse melanocytes pretreated with coffee blend. Evaluation of filaggrin synthesis by human primary epidermal keratinocytes pretreated with coffee blend. Evaluation of hyaluronic acid secretion by human primary epidermal keratinocytes pretreated with coffee blend.

Example 1: Effect on skin pigmentation In order to evaluate the potential beneficial effect of raw materials on skin depigmentation or pigmentation promotion, 1-melanogenesis evaluation test using two-dimensional culture of mouse melanocytes (B16) and Two tests were performed: 2-tyrosinase production evaluation test.

1. Cell culture conditions: B16 cells were cultured in DMEM 1 g / L glucose supplemented with 10% fetal bovine serum without using phenol red in a 37 ° C. humidified chamber containing 5% CO ₂ .

2. Production of melanin by B16 mouse melanocyte cell line Cells were incubated for 72 hours with selected raw materials or test reference (400 μg kojic acid / mL) in the presence or absence of NDP-MSH, an analog of MSH. The total amount of melanin (extracellular and intracellular) was assessed by measuring the optical density at 405 nm of each sample relative to the melanin standard in the presence or absence of NDP-MSH.

3. Production of tyrosinase by B16 mouse melanocyte cell line The cells were incubated for 48 hours with selected raw materials or test reference (400 μg / mL kojic acid). Tyrosinase production was assessed by immunolabeling.

raw materials:
For coffee blends comprising PROTECT® extract (Nestle non-roasted blend) and other blends, such as, for example, Svetol (manufactured by Naturex) and rice extract-P The test was done. The tested concentrations of each coffee extract are shown in Table 1.

  The maximum non-cytotoxic concentration was investigated so that the conditions between different extracts could be normalized as much as possible to allow further comparison of the extracts. Therefore, each coffee extract was tested at a concentration of 40 μg / mL.

Results The results are expressed as a percentage relative to the control. The test reference (kojic acid) induced a reduction in melanin content as expected. FIG. 1 shows melanin production by B16 melanocytes treated for 72 hours with selected raw materials.

  Overall, coffee extract was able to reduce the production of melanin and tyrosinase in vitro compared to control conditions (Figures 1 and 2), which may lead to skin depigmentation Was observed. Some extracts, such as protect and roasted coffee extracts, have lower melanin content without affecting tyrosinase production, suggesting a mechanism that acts upstream or downstream of tyrosinase.

Example 2 Skin Barrier Function and Effect on Hydration The skin barrier function and potential beneficial effects on skin hydration of the extract of Example 1 were evaluated using two-dimensional culture of human primary epidermal keratinocytes. Two studies were performed to evaluate the synthesis of 1-filaggrin and to evaluate the secretion of 2-hyaluronic acid by human primary epidermal keratinocytes.

Cell culture conditions Human epidermal keratinocytes were cultured in keratinocyte serum-free medium in a 37 ° C. humidified chamber containing 5% CO ₂ .

Synthesis of filaggrin by human epidermal keratinocytes Cells were incubated for 144 hours with selected raw materials or test reference (1.5 mM CaCl ₂ ). Filaggrin production was assessed by immunolabeling.

Secretion of Hyaluronic Acid by Human Epidermal Keratinocytes Cells were incubated for 72 hours with selected raw materials or test references (10 ^-7 M retinoic acid). The amount of hyaluronan released by human epidermal keratinocytes in the supernatant was quantified by ELISA assay.

Results The results are shown in FIGS. Pre-treatment of the cells with the coffee blend increased filaggrin by nearly 200%, suggesting that these extracts are capable of enhancing the skin barrier (Figure 3). Protect and light roasted coffee extract is a hyaluronic acid (Figure 4), secreted by skin cells in vivo, and a glycoconjugate that helps maintain proper hydration by drawing water into the extracellular space and capturing it. Secretion of the Saminoglycan was increased. The stronger the skin barrier, the more reliable the body can be protected from environmental and pathogen attack. Also, the skin barrier limits the loss of water through the epidermis and ensures proper skin hydration.

Example 3
Preparation of roasted seeds roasted coffee beans 0.5 kg of roasted extract preparation is powdered by grinding for 1 minute at 24000 rev / min, 4 ° C. (ice bath), using a Turrax device.

  The powder obtained is mixed with 5 liters of 0.05 M phosphate buffer, pH 8.5. The whole mixture is stirred for 30 minutes at 4 ° C. and then centrifuged at 10000 G, 4 ° C., and the supernatant is filtered through a 0.22 μm filter (sterile filtration).

  The extract is then fractionated by ultrafiltration through a Sartorius-type membrane in order to remove the oxidation phenomenon from the extract. The extract is then lyophilized. This gives 29.5 grams of active extract, called "lyophilized extract".

Caffeine is removed by supercritical chromatography (using CO ₂ as a carrier gas). This gives 25.5 grams of an active extract called "decaffeinated lyophilised extract".

Example 4: Powder Stick

Claims (15)

  Effect of a mixture of at least one roasted and non-roasted coffee bean or extract thereof as an active agent for preparing an oral composition for treating and / or preventing skin pigment abnormalities and / or skin defects Use for the purpose of beauty.   The use according to claim 1, characterized in that the skin diseases are those observed and / or stained in melasma, sputum egg spots, vitiligo, mottleflies, phenylketonuria, etc.   3. Use according to claim 1 or 2 for improving the color and / or gloss of the skin.   The use according to any one of claims 1 to 3 for lightening and / or whitening the color of the skin.   5. The use according to any one of the preceding claims, wherein the composition further improves skin hydration.   The use according to any of the preceding claims, wherein the composition further improves the barrier function of the skin.   In the above-mentioned mixture of non-roasted and roasted coffee beans, the weight ratio of non-roasted part to roasted part in coffee product is 1:99 to 90:10, preferably 30:70 to 50:50, more preferably 7. The use according to any one of the preceding claims, which is 35: 65 to 45: 55, most preferably 37: 63 to 42: 58.   The use according to any of the preceding claims, wherein the extract is derived from coffee beans selected from arabica-type coffee, Robusta-type coffee, Canephora-type coffee or Iberica-type coffee.   The use according to any of the preceding claims, wherein the coffee beans are decaffeinated.   10. The use according to any of the preceding claims, wherein the coffee bean extract comprises 10% to 100% of the total weight of the composition.   11. The use according to any of the preceding claims, wherein the coffee bean blend administered by the oral route is 0.01-5000 mg / day, preferably 0.5-1000 mg / day.   12. The use according to any one of the preceding claims, wherein the composition is a food product, a beverage, a food supplement, a nutraceutical or a pharmaceutical.   13. The use according to any one of the preceding claims, wherein the composition further comprises at least one food grade microorganism, in particular a dead, live or semi-active form of probiotics.   For the treatment and / or prevention of skin pigment abnormalities and / or skin defects comprising at least one step of administering to the individual an effective amount of a mixture of at least one of roasted and non-roasted coffee beans or an extract thereof Useful, cosmetic methods of treatment.   Roasted and non-roasted coffee beans or extracts thereof in combination with oral or topical compositions which may contain dead bacteria, live bacteria or semi-active forms of probiotic microorganisms or wettable powders or anti-aging agents A kit for treating and / or preventing skin pigment abnormality and / or skin defect, comprising an oral therapeutic agent comprising a composition containing at least one mixture of

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