CN103079525B - Fresh coffee beans are used for the purposes for adjusting skin pigmentation disorders - Google Patents
Fresh coffee beans are used for the purposes for adjusting skin pigmentation disorders Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P17/16—Emollients or protectives, e.g. against radiation
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/88—Two- or multipart kits
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Abstract
Present invention relates generally to the food supplement and/or field of food for cosmetic purpose.More specifically, the present invention is intended to provide a kind of include fresh coffee beans or the composition of its extract, for preventing and/or treating hyperpigmentation, skin color defect such as age mark and the other skin disorders for being characterised by pigment anomaly of skin.The present invention also aims to improve skin color and provide skin-whitening or brightening agent.
Description
Present invention relates generally to the food supplement and/or field of food for cosmetic purpose.More specifically, purport of the present invention
A kind of composition comprising fresh coffee beans or its extract is being provided, for preventing and/or treating hyperpigmentation, the skin of skin
Skin colour deficient such as age mark and the other skin disorders for being characterised by pigment anomaly.The present invention also aims to improve skin
The colour of skin, which is adjusted and provided, makes lightening skin or brightening agent.
Background of invention
Skin color is mainly what is determined by a kind of amount and type for brown pigmentation-- melanin being present in skin.
The fewer skin of amount of melanin is whiter, and it is deeper to measure more skin colors.In addition, the hyperpigmentation of skin is also by skin
Caused by the overexpression or accumulation of melanin.Therefore, the involved approach of melanin generation has become many and is used for reducing
Melanin produces the target spot of horizontal inhibitor.One of involved main enzyme is tyrosinase in melanin pathway.
The synthesis of melanin is a process controlled by hormone, including rush melanocyte is thin as caused by precursor POMC
Intracellular hormone (MSH) and corticotropin (ACTH) peptide.The process can be stimulated by the caused DNA damage of UVB irradiations.
Therefore, prolonged Exposure to Sunlight can cause many biochemical reactions in skin, cause such as skin tanning and solarization into brown
Color.Other consequences of Exposure to Sunlight can accumulate over time.These change can cause age mark appearance and produce it is uneven,
Mottled skin color.Unfortunately, or many products being commercially commercially available at present only have very weak effect, or
Containing unstable active component, these compositions can lose their effect when mixing in final formula.
In today's society, it is highly desirable to the expression of pigment in skin can be changed, to promote the uniform or thin out colour of skin.Perhaps
More people wish to change their colour of skin, to reduce age mark etc., or for pure cosmetic reasons.
As a result, the trial of exploitation compositions useful has all been concentrated on the material for suppressing tyrosinase activity.For example,
Have pointed out and use various tyrosinase inhibitors such as quinhydrones, vitamin C, cysteine, kojic acid, ursin and glutathione
In topical composition.In addition, also it has been proposed that being used to improve pigment anomaly (such as yellowish-brown by multiple dermatosis composition
What is observed in spot, freckle, leucoderma, piebaldism, PKU etc. those) looks and/or for cosmetic purpose.
In addition, the use of skin bleaching composition is also extended extensively.However, they or destroy melanin, either
Suppress its formation.Many such compositions contain harsh chemical agents, such as peroxide, acid or formaldehyde, or Thiolation
Material.The therapy of less causticity has other shortcomings.
It has been proposed that propose laser as pigment palliative, somebody using local retinoid and topical corticosteroid
Therapy and chemical peeling method, but be that can not obtain required response the shortcomings that these methods.
The it is proposed of some other composition uses natural materials on skin, and some of which natural materials are in Asia or Europe
Continent has used several centuries, for bleaching skin and skin area, or for strengthening the aesthetic feeling of fair skin.Including use
Lemon, orange, cucumber, ginkgo, carob, fructus rosae, geranium wilfordii, Chinese cassia tree, marjoram, rosemary etc..
In order to resist the illness relevant with abnormal pigment or in order that the colour of skin is thin out, it has been suggested that ought locally be applied using various
Tyrosinase activity can be reduced when being put on skin and therefore limits compound caused by melanin.Unfortunately, at present may be used
It is not entirely satisfactory with the method for disposal of use, particularly in terms of the side effect being frequently accompanied by, for example (,) it is some local living
The excitant side effect of property agent.
Therefore, it is highly desirable to other alternative preparations in the absence of those described in the prior art shortcomings.Specifically
Say on ground, it is highly desirable to develop the trophism cosmetic composition for being applied by oral route, it has improved stability
With promote even skin tone or make the thin out effect of the colour of skin.
In addition it is also necessary to for treat and/or prevent cutaneous pigmentation it is abnormal, especially because environmental factor or aging
The abnormal effective activating agent of those caused.
The purpose of the present invention meets these demands.
Summary of the invention
The present inventor can be by providing containing the food or food including at least fresh coffee beans or the composition of its extract
Supplement composition realizes the purpose.
Therefore, first topic of the invention be related to effective dose comprise at least fresh coffee beans or its extract into being allocated as
It is used to treating and/or preventing the abnormal cosmetic applications of cutaneous pigmentation for activating agent.The skin disorder be particularly those by
In exception caused by age or environment (such as ultraviolet) factor, such as age mark.It can also be in chloasma, freckle, white
What is observed in purplish or white patches on the skin wind, piebaldism, PKU etc. those.
The inventors discovered that fresh coffee beans can effectively suppress the formation (melanogenesis) of melanin, despite the fact that should
Active suppression of the extract to tyrosinase is very weak or does not suppress.In view of key of the tyrosinase in melanin generation
Effect and exploitation of the prior art are attempted all to concentrate in the suppression to the enzyme, and the result is wondrous and goes out people's will
Material.
For the purposes of the present invention, term " skin " refers to the skin of face or body.
For the purposes of the present invention, term " effective dose " refers to be enough the amount for obtaining Expected Results.
For the purposes of the present invention, term " prevention " refers to reduce the danger that the abnormal sign considered occurs
Property.
It is used to treat as activating agent the invention further relates to mentioned component and/or prevents skin splash (pigment
Imperfection cosmetic applications).As a result, the colour of skin become it is brighter evenly, without the region of dermatodyschroia or drying.
The invention further relates to the composition comprising fresh coffee beans or its extract at least of the invention of effective dose as activity
Agent is used to brighten or make the thin out cosmetic applications of skin color.
The present inventors have additionally discovered that composition of the invention can further improve moisture content of skin and/or skin barrier function.
The application of the present invention also includes comprising at least fresh coffee beans or the composition of its extract and at least one of effective dose
For further improving the activating agent of moisture content of skin or skin aging, being particularly the combination application of those following activating agents.
According on the other hand, subject of the present invention be in individual treat and/or prevent skin splash and with excessive color
Plain calm relevant illness, the particularly method of particularly aesthetic illness, beauty method, this method include it is at least one to
The individual applies the step of composition comprising fresh coffee beans or its extract at least of the invention.
The composition of the present invention can be taken orally.Its advantage is can be by the administration of rapid and relative unrestriction
Mode general action is in the deep layer (corium, hypodermis) of whole skin.Specifically, metabolin and other active nutritional materials pass through
The effect of blood flow is especially distributed in dermal matrix.Being administered orally also has the excellent of rapid and relative unrestriction administering mode
Point.
Detailed description of the invention
According to the first purpose, the invention provides the application of fresh coffee beans.
In follow-up text, " coffee bean " must be understood to refer to since coffee " cherry ", as described above
After peeling off, pass through wet method approach (fermentation, washing) or the beans obtained by dry method approach (drying, subsequent mechanical descaling).
" extract " must be understood to refer to all chemical combination obtained by the alcohol of crude product, water-alcohol or water-extraction
Thing.It is advantageous that it is selected from coffee for the species for preparing the coffee tree selected by coffee bean extract used in the composition
(Coffea) species.
Coffee tree is small-sized trees (10-15x4- that is the smooth of the edge, perennial, tough, having smooth leaf
6cm).White strong fragrant flower at axil it is verticillate in groups.Fruit for green drupe, its be changed into maturation it is red and
The berry of two plane convexs is usually contained, they are by its planar abutment.Although only two species (Arabica coffees
Coffee (C.Arabica) and middle fruit coffee (C.canephora)) supply coffee market basic need, but coffee tree is a lot
Species are present in the Tropical forests in East Africa with wild state.
It is (dry by wet method approach (fermentation, washing) or dry method approach coffee " beans " is drupe by coffee " cherry "
It is dry, then mechanical dejacketing) obtain.The reduction of pulp removes red peel and fresh middle pericarp；It causes coffee " shell ".Peel off
(endocarp for removing lignification removal) obtains coffee " berry " (or beans) afterwards.
Green coffee can be derived from me and block than coffee or Robusta's coffee (Robusta), or can be Arabica coffee
The blend of coffee and Robusta's coffee.Green coffee part can at least partly remove caffeine.Product as this offer, its
Caffeine level can be from going caffeine to conventional caffeine level to be adjusted.
Specifically, coffee bean extract can by the water of coffee bean, water-alcohol or alcohol extracting, preferably by means of methanol, second
The extraction of alcohol or propyl alcohol and obtain.Preferably, it, which is free of, can pass through the fraction of the coffee bean of non-polar solvent extract.For preparing
Go what the method for the coffee-extract of caffeine was well known in the art.
Composition according to the present invention can be any galenical form for being commonly available to selected application process.
Carrier can have heterogeneity, the type of this composition depended in considering.Correspondingly, composition can be any food
Or medicine, or the cosmetics for oral application.Food is used or the example of pharmaceutical carrier includes complete nutritional formula, dairy products
Such as breast, Yoghourt, cheese, acidified milk, newborn base fermented product, ice cream, cereal product, the newborn original washing powder of cereal product or fermentation
End, child and infant formula, freezing or resistance to storage beverage, candy foods, chocolate or cereal type, animal feed (particularly family
Support animal).Specifically, witloof or its extract can mix the food supplement or enriched food of any other form
In, such as food bar or compacting or non-compacted powder.Powder can be diluted in water, soda, dairy products or soybean derivatives, or incorporation
In food bar.
Food also can further include protein source, carbohydrate source, lipid source, mineral sources and/or vitamin source.Egg
The presence of white matter, carbohydrate, lipid, mineral and/or vitamin can have several advantages.These compounds typically facilitate
The taste and mouthfeel of finished product.They are also provided to body nutrient, and when body is influenceed by skin disorder, there is an urgent need to this battalion
Support element.They also allow the product configuration of the present invention to help nutrient formulation, so as to not need extra nutrition.
Single composition can include any kind of edible compound.The typical composition of food compositions, particularly beverage
It is well known in the art, such as breast, cream, tune coffee whiteners (coffee whitener) and coffee creamer (coffee
creamers).Alternatively, composition can also be such as salad dressing or part thereof.Such compound can be used for consumer
Change fragrance, outward appearance and the quality of such as composition.Composition can be liquid or dried forms, such as dissolve and/or be suspended in
Powder type in beverage.
Present invention also offers coffee beverage products, such as (a) is bakeed and ground coffee product, the production of (b) soluble coffee
Product, (c) single type coffee pad, piece or capsule, (d) instant drink type coffee product or (e) any other suitable coffee product, comprising
Coffee product as herein defined as composition.
Soluble coffee or further referred to as instant coffee are well-known and it commercially produces many years.
It is produced in a variety of ways by baking and ground coffee, and all this method are that those skilled in the art are well-known.
Instant invention is especially suited for as soluble coffee product or any product based on or from soluble coffee product.
For example, coffee product can also be used for so-called instant drink type beverage.The example for the instant drink type beverage that product of the present invention is applied to includes
Two-in-one beverage, it includes coffee element together with natural or artificial sweetening agent component, and the component is optionally with liquid such as water
Or newborn pre-dilution.In this case, coffee element can include the coffee product of the present invention.
Three-in-one beverage, it includes coffee, sweetener and brightening agent such as breast, liquid creamer or solid (such as powder) milk
Essence, the coffee product of the present invention can be included.
Moreover, the coffee product of the present invention can be included as any beverage of composition comprising soluble coffee.
Composition can further include more suitable for covering the composition in food compositions.Conventional composition can be example
Such as sugar, artificial sweetener, emulsifying agent, stabilizer, thickener, flowable, pigment (colors), flavor enhancement, aromatic
(aromas) etc..Suitable artificial sweetener includes saccharin, cyclamate class (cyclamates), acesulfame potassium
(acetosulfame), the Sweetening agents such as aspartame based on L- aspartyl bases, and its mixture.Suitable emulsifying agent bag
Include monoglyceride, two glyceride, lecithin, the diacetyl tartrate of list-double glyceride, emulsified starch and its mixture.It is suitable
The stabilizer of conjunction includes dipotassium hydrogen phosphate and sodium citrate.Suitable flowable is sodium silicoaluminate.In one embodiment, group
Compound includes lactoprotein and/or phytoprotein.In further embodiment, composition includes butter oil and/or plant
Thing fat.
Cook thing (standard brew), espresso (espresso) in addition, the present invention can be used as standard, criticize
Measure the composition of the beverage preparation using or being used in the single portion of beverage prepared for family based on coffee.
If the product is the nutritious supplementary pharmaceutical for orally administering, it may be present in capsule, gelatine capsule, flexible glue
In capsule, tablet, sugar coated tablet, pill, paste or lozenge, chewing gum or drinkable solution or emulsion, syrup or gel.This
Class replenishers can also include sweetener, stabilizer, antioxidant, additive, flavor enhancement and/or colouring agent.Its preparation passes through
Common method for producing sugar coated tablet, gelatine capsule, gel, controlled release hydrogel, emulsion, tablet or capsule is carried out.
In this aspect, the adjuvant for Orally administered composition, particularly for nutritious supplementary pharmaceutical is known to professional.Especially
And solely for illustrative purposes, it can be mentioned that being generally used for the lubricant such as magnesium stearate of food, for instant solubilising
Product, gelling agent, thickener, humidizer, fat and/or aqueous compounds, preservative, adjusting material (texturizing), seasoning
Agent and/or coating agent, antioxidant and coloured material.
In addition, according to the present invention composition can contain lipid, polyphenol, taurine, probiotic micro-organisms, vitamin and/
Or trace element.If, can be in the form of living, partly form living or inactive forms, such as with freeze-dried powder using probiotics
Form includes them.
The culture supernatant of microorganism may also include in composition.They may be selected from lactic acid bacteria, particularly lactobacillus
And/or Bifidobacterium, and it is more preferably selected from Yue Shi lactobacillus (Lactobacillus johnsonii), Luo Yishi breast bars
Bacterium (Lactobacillus reuteri), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Lactobacillus paracasei
It is (Lactobacillus paracasei), Lactobacillus casei, bifidobacterium bifidum (Bifidobacterium bifidum), short
Bifidobacterium Bifidum (Bifidobacterium breve), animal bifidobacteria (Bifidobacterium animalis), Ying Ershuan
Discrimination bacillus (Bifidobacterium infantis), bifidobacterium adolescentis (Bifidobacterium dolescentis) and
False chainlet Bifidobacterium (Bifidobacterium pseudocatenulatum).It is used according to the most preferred embodiment
Bacterial strain be Institut Pasteur is preserved on June 30th, 1992 according to budapest treaty, preserving number is CNCM 1-
1225 Yue Shi lactobacillus (La1) or be preserved according to budapest treaty on January 12nd, 1999 Institut Pasteur,
Preserving number is CNCM I-2116 Lactobacillus paracasei (ST11).Following compounds can for example be used alone or in combination.Zinc and its
Salt including zinc sulfate and zinc gluconate (zinc glucanate), vitamin B5, B6, B8, C, E or PP, p- carrotene and
Carotenoid, garlic P.E (particularly diallyl sulfide or garlic oil form), selenium, curcumin, curcuminoids, cigarette
Acid, alkannic acid (lithospermic acid) and adenosine.It should be understood that professional can select such reactive compound, if any can
They can be combined in this way, by preventing required targeted activity to be suppressed or weakening to improve as mesh of the present invention
Composition intended effect.
Using
The product of the present invention can be efficiently used for treating or preventing skin pigment for example, by reducing the generation of melanin
It is calm abnormal or be cosmetically used to making the colour of skin thin out etc..Really, raw coffee bean extract show can reduce in vitro it is black
The synthesis (embodiment 1, Fig. 1) of pigment.The generation of tyrosinase is also reduced, but limitation (Fig. 2), and this shows subtracting for melanin
The suppression of tyrosinase is not due to less, and is due to the mechanism of the upstream or downstream that act on the enzyme.
The composition of the present invention also has positive role to reinforcing skin barrier and skin moisture-keeping.
As a result, color spot is reduced, the colour of skin become it is brighter evenly, without the region of dermatodyschroia or drying.
Therefore, a theme of the invention is related at least one composition comprising fresh coffee beans of effective dose as activating agent
For treating and/or preventing cutaneous pigmentation exception, especially because age or environmental factor are for example UV-induced different
Normal cosmetic applications.
It is used to brighten or makes as activating agent the invention further relates at least one composition comprising fresh coffee beans of effective dose
The thin out cosmetic applications of the colour of skin, this is that Asian particularlys hope.
The application of the present invention also includes at least one composition comprising fresh coffee beans and at least one of effective dose is used to change
The activating agent of kind moisture content of skin or skin aging, the combination application of those particularly following activating agents.
According on the other hand, subject of the present invention be in individual treat and/or prevent skin splash and with excessive color
Plain calm relevant illness, the particularly method of particularly aesthetic illness, beauty method, this method include it is at least one to
The step of individual applies at least one composition comprising fresh coffee beans of the invention.
The cosmetic treatment method of the present invention can be especially through at least one present invention at least effect amount that orally administers
The composition comprising fresh coffee beans carry out.Orally administer including once or several times taking in Oral compositions defined above
Thing.
It can include single application.According to another embodiment, using can repeat daily such as 2 to 3 times, continue
One day or more day, and typically last at least 4 weeks or 1 to 15 week.
In addition, in order to supplement or strengthen the activity of composition defined in the present invention, it is also contemplated that it is optional with it is oral or
The combined therapy of localized forms.
Therefore, the composition comprising fresh coffee beans of the invention is with optionally containing another active component, particularly benefit
Raw microorganism or other dead, living or half form living probiotics or the orally or topically compositions of NMF or antidotal agent
The part or oral medication of combination can be counted as medicine box.Before preparation, each composition is held very much according to those skilled in the art
The order and condition easily determined is mixed.
Certainly, determine coffee bean extract in the composition the effect needed for and the mode of administration of composition
Ratio.
Between the daily dose for the raw coffee bean extract applied by oral route is preferably included in 0.1 to 27g/ days,
It is even more preferably 1 to 3g/ days depending on product type and content of caffeine, more preferably 0.5 to 18g/ days.The present invention
People has found：Validity according to the raw coffee bean extract of the present invention be typically dose dependent and follow dose response
Curve.If preventing generally gentle skin disorder or damage and will continually use product, considerably less amount can foot
With effect needed for realization.If treating serious skin pigment illness, bigger amount can be more suitable, although can also produce on a small quantity
Coming into force should.
Preferably, it is contemplated that composition for oral administration contain scope be composition weight 1% to 100% and preferably
The coffee bean extract of the amount of the 10% to 80% of composition weight, this depends on product form.
This it appears that the other advantages and features of the present invention from following embodiment and accompanying drawing.Therefore, it is real below
Example is applied to be used to illustrate the field of the invention in a non limiting manner.Unless otherwise indicated, percentage in these embodiments
It is percentage by weight, the number range write out with " ... extremely ... " includes the upper and lower bound particularly pointed out.
Accompanying drawing
Fig. 1：The assessment that the melanin of the mouse melanocyte pre-processed with green coffee extract generates.
Fig. 2：The assessment that the tyrosinase of the mouse melanocyte pre-processed with green coffee extract generates.
Fig. 3：The silk polyprotein of the primary epidermal keratinocytes of people pre-processed with different green coffee extracts
(filaggrin) assessment of synthesis.
Fig. 4：The assessment of the hyaluronic acid secretion of the primary epidermal keratinocytes of people pre-processed with green coffee extract.
Fig. 5：The CD44 of the human epidermic keratinocyte pre-processed with different green coffee extracts is generated.
Embodiment
Embodiment 1：Influence to cutaneous pigmentation
In order to assess composition to depigmentation of skin or promote pigmented potentially beneficial effect, we use mouse melanocyte
2D cultures (B16) have carried out 2 experiments：1- assesses melanin generation and 2- assesses tyrosinase generation.
1.Cell culture condition
By B16 cells containing 1g/L glucose, without it is phenol red, be supplemented with the DMEM of 10% hyclone, be humidified
At 37 DEG C, containing 5%CO in culturing room2Under conditions of cultivate.
2.Melanin is generated by B16 mouse melanocyte system
By cell with selected composition or experiment object of reference (kojic acid, 400 μ g/mL) together presence or absence of MSH classes
Incubated 72 hours like under conditions of thing NDP-MSH.The total amount (extracellular and intracellular) of melanin is each by being determined in 405nm
Sample and melanin standard condition are assessed than the optical density under the conditions of presence or absence of NDP-MSH.
3.Tyrosinase is generated by B16 mouse melanocyte system
Cell and selected composition or experiment object of reference (kojic acid, 400 μ g/mL) are incubated 48 hours together.By immune
Labelling method assesses the generation of tyrosinase.
Composition
We have tested the green coffee extract for removing caffeine of Robusta and Arabica,(by
Naturex is provided)；Oryza ' s Extract-P and the green coffee of water extraction.The test concentrations of every kind of green coffee extract are shown in
In table 1.
Table 1：Green coffee extract is to the primary dermal fibroblast of people and to the thin of the primary epidermal keratinocytes of people
Cellular toxicity
We have studied highest no cytotoxicity concentration, standardize the condition between different extracts as far as possible to enter one
Step can compare them.Therefore, the coffee-extract of 40 μ g/mL concentration has been tested.
As a result
As a result to be represented relative to the percentage of control.As is expected, experiment object of reference (kojic acid) causes black and contained
The reduction of amount.Fig. 1 shows the melanin generated with the B16 melanocytes of selected composition processing 72 hours.
Generally speaking, we have observed that coffee-extract is calm to skin depigmenting to have some potentiality because with it is right
Compared according to condition, they can reduce the external generation (Fig. 1 and 2) of melanin and tyrosinase.
Embodiment 2：Influence to skin barrier function and water content
The 2D cultures of the primary epidermal keratinocytes of user assess the extract of embodiment 1 to skin barrier work(
Can be with the potentially beneficial effect of moisture content of skin, we carry out 3 experiments：1- assesses to be closed by the primary epidermal keratinocytes of people
Into silk polyprotein, 2- assesses to be assessed by the primary epidermis of people by the hyaluronic acid and 3- of the secretion of people's primary epidermal keratinocytes
The CD44 (hyaluronic acid receptor) of keratinocyte generation.
Cell culture condition
Human epidermic keratinocyte is trained in restricted (control) keratinocyte SFM culture mediums, in humidification
Support in room at 37 DEG C, containing 5%CO2Under conditions of cultivate.
Silk polyprotein is synthesized by human epidermic keratinocyte
By cell and selected composition or experiment object of reference (CaCl2, 1.5mM) incubate 144 hours together.Pass through immune mark
Notation assesses the generation of silk polyprotein.
Hyaluronic acid is secreted by human epidermic keratinocyte
By cell and selected composition or experiment object of reference (retinoic acid, 10-7M) incubate 72 hours together.Surveyed by ELISA
Determine standard measure hyaluronic acid as caused by the human epidermic keratinocyte in supernatant to discharge.
CD44 is generated by human epidermic keratinocyte
By cell and selected composition or experiment object of reference (retinoic acid, 10-7M 72 hours) are incubated together.Pass through immune mark
Notation evaluation CD44 as caused by human epidermic keratinocyte is generated.
As a result
As a result as shown in Fig. 3,4 and 5.Green coffee, which is shown, adds a polyprotein generation (Fig. 3).The life of caffeine is gone to spread out
This tower and arabica coffees extract moderately add hyaluronic acid secretion (respectively compare 128% and 120%) (figure
4), hyaluronic acid is a kind of by the endocrine glycosaminoglycan of Skin Cell body, and it is by luring and retaining moisture in cell outer room
Contribute to maintain appropriate water content in gap.However, Oryza and the green coffee of water extraction are added and (respectively compareed
148% and the 132%) generation (Fig. 5) of CD44- hyaluronic acid receptors.Stronger skin barrier ensures that preferably protection body is exempted from
Attacked by environment and pathogen.It also limits the loss of moist by epidermis, ensures that suitable moisture content of skin.
Claims (13)
1. the raw coffee bean extract for removing caffeine of effective dose is used to prepare for treating and/or preventing as sole active
The purposes of skin pigmentation disorders and the oral cosmetic composition for further improving the moisture content of skin, wherein going
The raw coffee bean extract of caffeine is extracted by the water of coffee bean to obtain.
2. purposes according to claim 1, wherein described skin pigmentation disorders are skin splash.
3. purposes according to claim 1, wherein the skin pigmentation disorders are the skin observed in chloasma, freckle
Illness, and/or age mark.
4. purposes according to claim 1, wherein described oral cosmetic composition is used to make lightening skin and/or brighten
Skin color.
5. purposes according to claim 1, wherein the extract is derived from coffee bean, the coffee bean is selected from Arabica coffee
Coffee, Robusta's coffee, middle fruit coffee or Coffea iberica species.
6. purposes according to claim 1, wherein the coffee bean extract accounts for the 1% to 100% of the composition gross weight.
7. purposes according to claim 1, wherein the composition is food or medicine.
8. purposes according to claim 7, wherein the composition is beverage or food additives.
9. purposes according to claim 7, wherein the composition is nutriment.
10. purposes according to claim 1, wherein the composition further includes the micro- life of food-grade of at least one type
Thing.
11. purposes according to claim 10, wherein the food-grade microorganisms are dead, living or partly form living prebiotic
Bacterium.
12. (i) contains Orally administered composition of the fresh coffee beans water extract of caffeine as sole active, and (ii) is optionally
The Orally administered composition that ground contains dead, living or half form living probiotic micro-organisms is used to prepare treatment and/or prevention skin
The purposes of pigmentation disorders and the kit for further improving the moisture content of skin.
13. purposes according to claim 12, wherein described skin pigmentation disorders are skin splash.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10171376.6 | 2010-07-30 | ||
| EP10171376 | 2010-07-30 | ||
| PCT/EP2011/063088 WO2012013776A2 (en) | 2010-07-30 | 2011-07-29 | Use of green coffee beans for regulating skin pigmentation disorders |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103079525A CN103079525A (en) | 2013-05-01 |
| CN103079525B true CN103079525B (en) | 2018-03-09 |
Family
ID=43416787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180037623.0A Expired - Fee Related CN103079525B (en) | 2010-07-30 | 2011-07-29 | Fresh coffee beans are used for the purposes for adjusting skin pigmentation disorders |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP2598102A2 (en) |
| JP (1) | JP2013533282A (en) |
| CN (1) | CN103079525B (en) |
| BR (1) | BR112013002215B1 (en) |
| MX (1) | MX350475B (en) |
| RU (1) | RU2013108968A (en) |
| WO (1) | WO2012013776A2 (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10500187B2 (en) | 2012-09-21 | 2019-12-10 | Societe Des Produits Nestle S.A. | Composition comprising plant phenols for preventing or reducing TEWL and associated disorders and diseases |
| US20150209399A1 (en) * | 2013-12-05 | 2015-07-30 | Applied Food Sciences, Inc. | Methods For Enhacement Of Dehydroepiandrosterone Using Green Coffee Bean Extract |
| US10806769B2 (en) | 2016-03-31 | 2020-10-20 | Gojo Industries, Inc. | Antimicrobial peptide stimulating cleansing composition |
| AU2017240069B2 (en) | 2016-03-31 | 2024-03-07 | Gojo Industries, Inc. | Sanitizer composition with probiotic/prebiotic active ingredient |
| BR102016019123A2 (en) * | 2016-08-17 | 2018-03-06 | Natura Cosméticos S.A. | ANTI-SIGNAL COSMETIC COMPOSITION, USE OF THE COMPOSITION, ANTI-SIGNAL TREATMENT METHOD AND METHOD FOR CLEARING EYES AND / OR TREATMENT OF EYE BAGS |
| US11564879B2 (en) | 2016-11-23 | 2023-01-31 | Gojo Industries, Inc. | Sanitizer composition with probiotic/prebiotic active ingredient |
| US20200222358A1 (en) | 2019-01-15 | 2020-07-16 | Derma Research Group Inc. | Topical dermatologic acne treatment cream composition and method of manufacture |
| CN113729034A (en) * | 2021-07-30 | 2021-12-03 | 阿特美生物科技(海南)有限公司 | Air disinfectant of binary spray plant extract and preparation method thereof |
| FR3135992A1 (en) * | 2022-05-25 | 2023-12-01 | L'oreal | Molecular signature of actinic lentigo associated with vesicle management |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004026287A1 (en) * | 2002-09-09 | 2004-04-01 | Nestec S.A. | Orally administrable composition for improving skin quality |
| US20090169651A1 (en) * | 2007-06-28 | 2009-07-02 | Muhammed Majeed | Anti-skin damage compositions with complimentary dual mode of action |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0826967A (en) * | 1994-07-13 | 1996-01-30 | Ichimaru Pharcos Co Ltd | Cosmetic containing chlorogenic acid or its derivative |
| JPH0892057A (en) * | 1994-09-16 | 1996-04-09 | Ichimaru Pharcos Co Ltd | Cosmetic blended with extract of seed of coffee tree |
| JPH09151130A (en) * | 1995-12-01 | 1997-06-10 | Yakurigaku Chuo Kenkyusho:Kk | Medicine for treating trichopoliosis and leukoplakia |
| CA2508712A1 (en) * | 2002-12-13 | 2004-07-01 | L'oreal | Use of an extract of decaffeinated coffee beans in the preparation of a composition intended to stimulate the sebaceous function of the skin by oral administration |
| FR2848448B1 (en) * | 2002-12-13 | 2007-04-06 | Oreal | USE OF DECAFFEIN COFFEE GRAIN EXTRACT IN THE PREPARATION OF A COMPOSITION FOR STIMULATING THE SEBACEOUS FUNCTION OF THE SKIN BY ORAL ADMINISTRATION |
| JP2006335758A (en) * | 2003-10-06 | 2006-12-14 | Oriza Yuka Kk | Composition for diet |
| ATE525080T1 (en) * | 2003-10-06 | 2011-10-15 | Oryza Oil & Fat Chem | DIETARY COMPOSITION |
| DK1753387T3 (en) * | 2004-04-08 | 2012-09-10 | Vdf Futureceuticals Inc | Cosmetic compositions of coffee berries and methods |
| AR063304A1 (en) * | 2006-10-13 | 2009-01-21 | Gervais Danone Sa | NEW COMPOSITION TO IMPROVE SKIN QUALITY AND PROCESS TO PREPARE THE SAME |
-
2011
- 2011-07-29 WO PCT/EP2011/063088 patent/WO2012013776A2/en active Application Filing
- 2011-07-29 MX MX2013001078A patent/MX350475B/en active IP Right Grant
- 2011-07-29 CN CN201180037623.0A patent/CN103079525B/en not_active Expired - Fee Related
- 2011-07-29 JP JP2013521158A patent/JP2013533282A/en active Pending
- 2011-07-29 BR BR112013002215-9A patent/BR112013002215B1/en not_active IP Right Cessation
- 2011-07-29 RU RU2013108968/15A patent/RU2013108968A/en not_active Application Discontinuation
- 2011-07-29 EP EP11736394.5A patent/EP2598102A2/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004026287A1 (en) * | 2002-09-09 | 2004-04-01 | Nestec S.A. | Orally administrable composition for improving skin quality |
| US20090169651A1 (en) * | 2007-06-28 | 2009-07-02 | Muhammed Majeed | Anti-skin damage compositions with complimentary dual mode of action |
Also Published As
| Publication number | Publication date |
|---|---|
| MX2013001078A (en) | 2013-03-12 |
| EP2598102A2 (en) | 2013-06-05 |
| WO2012013776A2 (en) | 2012-02-02 |
| CN103079525A (en) | 2013-05-01 |
| MX350475B (en) | 2017-09-07 |
| WO2012013776A3 (en) | 2012-03-22 |
| BR112013002215B1 (en) | 2017-12-19 |
| BR112013002215A2 (en) | 2016-05-24 |
| RU2013108968A (en) | 2014-09-10 |
| JP2013533282A (en) | 2013-08-22 |
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