CN103079525A - Use of green coffee beans for regulating skin pigmentation disorders - Google Patents

Use of green coffee beans for regulating skin pigmentation disorders Download PDF

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CN103079525A
CN103079525A CN2011800376230A CN201180037623A CN103079525A CN 103079525 A CN103079525 A CN 103079525A CN 2011800376230 A CN2011800376230 A CN 2011800376230A CN 201180037623 A CN201180037623 A CN 201180037623A CN 103079525 A CN103079525 A CN 103079525A
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skin
coffee
aforementioned
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CN103079525B (en
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K·克雷亨比尔
O·莫罗克斯
A·牟迪克里弗
F·迪欧尼斯
M·吉塔尔
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Societe des Produits Nestle SA
LOreal SA
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A—HUMAN NECESSITIES
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/88—Two- or multipart kits

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Abstract

The present invention relates generally to the field of food supplements and/or food products for cosmetic purpose. More specifically, the present invention aims to provide an ingredient containing green coffee beans or an extract thereof for preventing and/or treating hyper-pigmentation of skin, skin color imperfections such as age-spots and other skin disorders characterized by abnormal pigments. The present invention also aims at improving skin tone as well as providing a skin lightening or whitening agent.

Description

Raw coffee bean is used for the purposes of regulation of skin pigmentation disease
The present invention relates generally to for the food supplement of cosmetic purpose and/or field of food.More specifically, the present invention aims to provide a kind of composition that comprises raw coffee bean or its extract, and the hyperpigmentation, skin color defective that is used for preventing and/or treating skin be senile plaque and be characterised in that other skin disorder of pigment anomaly for example.The present invention also aims to improve skin color and provide skin-whitening or brightening agent.
Background of invention
Skin color mainly is by a kind of brown pigmentation that is present in the skin---melanic amount and type decided.The fewer skin of melanic amount is whiter, and it is darker to measure more skin colors.In addition, the hyperpigmentation of skin is also by melanic overexpression in the skin or gather and cause.Therefore, melanin produces related approach has become many target spots that are used for reducing the inhibitor of melanin generation level.One of related main enzyme is tryrosinase in the melanin approach.
Melanic synthesizing is a process that is subjected to hormone control, comprises the melanotropin (MSH) and thyroliberin (ACTH) peptide that are produced by the precursor proopiomelanocortin.Shine caused DNA damage by UVB and can stimulate this process.
Therefore, the Exposure to Sunlight meeting causes the many biochemical reactions in the skin for a long time, causes for example skin tanning and tanning.Other consequence of Exposure to Sunlight can be accumulated along with the time.These variations can cause the appearance of senile plaque and produce inhomogeneous, mottled skin color.Can lose their effectiveness when unfortunately, many products that can buy at present or very weak effect is only arranged, or contain unsettled active component, these compositions are in mixing final prescription on market.
At society, very wish to change the expression of pigment in the skin, to promote evenly or the colour of skin of whitening.Many people wish to change their colour of skin, reducing senile plaque etc., or for pure cosmetic reasons.
Consequently, the trial of exploitation compositions useful has all concentrated on the material of restraint of tyrosinase activity.For example, proposed various tyrosinase inhibitors such as hydroquinone, vitamin C, cysteine, kojic acid, arbutin and glutathion etc. are used for topical composition.In addition, also the someone propose with multiple dermatosis learn compositions be used for improving pigment anomaly (such as chloasma, freckle, vitiligo, piebaldism, phenylketonuria etc. viewed those) looks and/or be used for cosmetic purpose.
In addition, the use of skin bleaching compositions is also extensively extended.Yet, they or destroy melanin, or suppress its formation.Many such compositionss contain the causticity chemical substance, for example peroxide, acid or formaldehyde, or the material of mercaptan.Not too the therapy of causticity has other shortcoming.
Someone proposes to use local retinoid and local corticosteroid as the pigment palliative, and the somebody has proposed laser therapy and chemical peeling method, but the shortcoming of these methods is to obtain required response.
Some other compositions proposes to use natural materials at skin, wherein some natural materials in the Asia or Europe used several centuries, be used for bleaching skin and skin area, or be used for strengthening the aesthetic feeling of pale skin.Comprising using Fructus Citri Limoniae, orange, Fructus Cucumidis sativi, Semen Ginkgo, carob, Rosehips, Herba Erodii, Cortex Cinnamomi, Origanum majorana L., Herba Rosmarini Officinalis etc.
In order to resist the disease relevant with abnormal pigment or for the whitening colour of skin, to have proposed to use the various chemical compounds that when the part spreads upon on the skin, can reduce tyrosinase activity and therefore limit the melanin generation.Unfortunately, the method for disposal that can adopt at present can not be entirely satisfactory, particularly aspect the side effect of often following, and for example zest side effect of some Topically active agent.
Therefore, very wish not exist other alternative preparation of those shortcomings of putting down in writing in the prior art.Specifically, wish very much to develop for the trophism cosmetic composition of using by oral route, it has the stability of improvement and promotes the even colour of skin or U.S. fair-complexioned effectiveness.
In addition, also need to for treat and/or prevent cutaneous pigmentation unusually, particularly because environmental factors or old and feeble caused those unusual effective activating agents.
Purpose of the present invention has satisfied these demands.
Summary of the invention
The inventor can realize this purpose by the food or the dietary supplement compositions that contain the composition that comprises at least raw coffee bean or its extract are provided.
Therefore, the first topic of the present invention composition that comprises at least raw coffee bean or its extract that relates to effective dose is used for the treatment of as activating agent and/or prevents the unusual cosmetic applications of cutaneous pigmentation.Described skin disorder particularly those because that age or environment (for example ultraviolet) factor cause is unusual, senile plaque for example.It can also be in chloasma, freckle, vitiligo, piebaldism, phenylketonuria etc. viewed those.
The inventor finds, raw coffee bean can the melanic formation of establishment (melanogenesis), although in fact this extract is very weak or do not suppress to the inhibition of the activity of tryrosinase.Consider that pivotal role and the of the prior art exploitation of tryrosinase in melanin generates attempt all concentrating on this enzymeinhibition, this result is wondrous and beat all.
For purpose of the present invention, term " skin " refers to the skin of face or health.
For purpose of the present invention, term " effective dose " refers to be enough to obtain the amount of Expected Results.
For purpose of the present invention, term " prevention " refers to reduce the danger of the unusual sign appearance of considering.
The invention still further relates to mentioned component is used for the treatment of and/or prevents skin splash (pigment imperfection) as activating agent cosmetic applications.Consequently, it is brighter more even that the colour of skin becomes, and do not have dyschromasia or dry zone.
The composition that comprises raw coffee bean or its extract of the present invention at least that the invention still further relates to effective dose is used for brightening as activating agent or the cosmetic applications of skin whitening tone.
The inventor finds that also composition of the present invention can further improve moisture content of skin and/or skin barrier function.
Application of the present invention also comprises the composition that comprises at least raw coffee bean or its extract and at least a activating agent, the particularly applied in any combination of those following activating agents for further improving moisture content of skin or skin aging of effective dose.
According on the other hand, theme of the present invention is method, the particularly beauty method that treats and/or prevents skin splash and disease, the particularly aesthetic disease relevant with hyperpigmentation in individuality, and the method comprises that at least one uses the step that comprises the composition of raw coffee bean or its extract of the present invention at least to described individuality.
Compositions of the present invention can be taken orally.Its advantage is can be by the deep layer (corium, hypodermis) of rapid and relatively unrestricted administering mode general action in whole skin.Specifically, metabolite and other active nutritional material are distributed in the dermal matrix especially by the effect of blood flow.Oral administration also has the advantage of rapid and relative unrestriction administering mode.
Detailed Description Of The Invention
According to the first purpose, the invention provides the application of raw coffee bean.
In follow-up text, " coffee bean " must be interpreted as referring to from coffee " Fructus Pruni pseudocerasi ", after aforesaid peeling off, by wet method approach (fermentation, washing) or the bean that obtains by dry method approach (drying, subsequently mechanical descaling).
" extract " must be interpreted as referring to all chemical compounds of being obtained by alcohol, water-alcohol or the water of crude product-extraction beginning.Advantageously, be selected from coffee (Coffea) species for preparation kind of the used selected coffee tree of coffee bean extract in compositions.
Coffee tree is small-sized trees entire, perennial, tough, that have smooth leaf (10-15x4-6cm).The flower of the strong fragrance of white is in groups verticillate at the axil place.Fruit is green drupe, it becomes redness and usually contains two plane convexs when maturation berry, and they are by its plane adjacency.Although only have the fundamental need in two species (arabica coffees (C.Arabica) and middle fruit coffee (C.canephora)) supply coffee market, a lot of species of coffee tree are present in the Tropical forests in East Africa with wild state.
Coffee " bean " is that drupe begins by coffee " Fructus Pruni pseudocerasi ", obtains by wet method approach (fermentation, washing) or dry method approach (drying, then mechanical dejacketing).Red peel and fresh mesocarp are removed in the minimizing of sarcocarp; It causes coffee " shell ".Peel off and obtain coffee " berry " (or bean) after (removing lignified endocarp).
Green coffee can be derived from me and block than coffee or Robusta's coffee (Robusta), maybe can be the blend of arabica coffees and Robusta's coffee.The green coffee part can at least part ofly be removed caffeine.This provides such goods, and its caffeine level can be regulated from going caffeine to conventional caffeine level.
Specifically, coffee bean extract can be by water, water-alcohol or the alcohol extraction of coffee bean, preferably the extraction by means of methanol, ethanol or propanol obtains.Preferably, it does not contain the fraction that can pass through the coffee bean of non-polar solvent extract.Method for the preparation of the coffee-extract that removes caffeine is well known in the art.
Can be any galenical form that usually is applicable to selected application process according to compositions of the present invention.Carrier can have heterogeneity, and this depends on the type of the compositions in the consideration.Correspondingly, compositions can be any food or medicine, or is used for the cosmetics of oral application.Food with or the example of pharmaceutical carrier comprise complete nutritional formula, milk product for example breast, yoghourt, cheese, fermentation milk, newborn basic fermented product, ice cream, cereal product or the cereal product of fermentation, newborn based powders, child and infant formula, freezing or the beverage of anti-storage the, candy foods, chocolate or frumentum type, animal feed (particularly domestic animal).Specifically, Herba Cichorii or its extract can mix in the food supplement or enriched food of any other form, for example food stick or compacting or not compacted powder.Powder can be diluted in water, soda, milk product or soybean derivatives, or mixes in the food stick.
Food also can further comprise protein source, carbohydrate source, lipid source, mineral source and/or vitamin source.The existence of protein, carbohydrate, lipid, mineral and/or vitamin can have several advantages.These chemical compounds help taste and the mouthfeel of finished product usually.They also offer the health nutrient, when health is affected by skin disorder in the urgent need to this nutrient.They also allow product configuration of the present invention to help nutrient formulation, so that do not need extra nutrition.
Single composition can comprise the edible compound of any type.The typical composition of food compositions, particularly beverage is well known in the art, for example breast, butter, accent coffee white oil (coffee whitener) and coffee cream (coffee creamers).Alternatively, composition also can be for example salad dressing or its part.Consumer can come with this compounds fragrance, outward appearance and the quality of Change Example such as compositions.Composition can be liquid or dried forms, for example dissolves and/or is suspended in powder type in the beverage.
The present invention also provides coffee beverage products, for example (a) cures and ground coffee product, (b) soluble coffee product, (c) single type coffee pad, sheet or capsule, (d) instant drink type coffee product or (e) any coffee product that other is fit to, and comprises the as herein defined coffee product as composition.
Soluble coffee or be called in addition instant coffee is well-known and it was commercially produced existing a lot of years.Its by cure and ground coffee with several different methods production, all the method are that those skilled in the art are well-known.
The present invention be particularly suitable for as soluble coffee product or any based on or be derived from the product of soluble coffee product.For example, coffee product also can be used for so-called instant drink type beverage.The example of the instant drink type beverage that product of the present invention is applicable to comprises two-in-one beverage, and it comprises the coffee component together with natural or artificial sweetening agent component, and described component is randomly with for example water or the pre-dilution of breast of liquid.In this case, the coffee component can comprise coffee product of the present invention.
Three-in-one beverage, it comprises coffee, sweeting agent and brightening agent for example breast, liquid cream or solid (for example powder) cream, can comprise coffee product of the present invention.
And, comprise soluble coffee and can comprise coffee product of the present invention as any beverage of composition.
Compositions can further comprise the composition that more is suitable for covering in the food compositions.Composition commonly used can be such as sugar, artificial sweetener, emulsifying agent, stabilizing agent, thickening agent, flowable, pigment (colors), flavoring agent, aromatic (aromas) etc.The artificial sweetener that is fit to comprises glucide, cyclamate class (cyclamates), acesulfame potassium (acetosulfame), based on the sweeting agent of L-aspartyl aspartame for example, and composition thereof.The emulsifying agent that is fit to comprises diacetyl tartrate, emulsified starch and its mixture of monoglyceride, two glyceride, lecithin, list-double glyceride.The stabilizing agent that is fit to comprises dipotassium hydrogen phosphate and sodium citrate.The flowable that is fit to is sodium aluminosilicate.In one embodiment, compositions comprises lactoprotein and/or phytoprotein.In further embodiment, compositions comprises butter oil and/or plant fat.
In addition, the present invention can be used as standard boiling thing (standard brew), Italian type concentrated coffee (espresso), the composition based on the beverage preparation of coffee that use in batches or that use at the single portion of beverage that is used for family's preparation.
If this product is for Orally administered supplementary, then it can be present in capsule, gelatine capsule, soft capsule, tablet, sweet tablet tablet, pill, paste or lozenge, chewing gum or drinkable solution or emulsion, syrup or the gel.These class supplement can also comprise sweeting agent, stabilizing agent, antioxidant, additive, flavoring agent and/or coloring agent.Its preparation is by carrying out for the production of the common method of sweet tablet tablet, gelatine capsule, gel, controlled release hydrogel, Emulsion, tablet or capsule.
Aspect this, be used for Orally administered composition, be known to the professional especially for the adjuvant of supplementary.Especially and only property purpose presented for purpose of illustration, can mention be generally used for food lubricant for example magnesium stearate, be used for product, gellant, thickening agent, humidizer, fat and/or aqueous compounds, antiseptic, adjusting material (texturizing), flavoring agent and/or coating materials, antioxidant and the coloured material of instant solubilising.
In addition, can contain lipid, polyphenol, taurine, probiotic micro-organisms, vitamin and/or trace element according to compositions of the present invention.If the use probiotic bacteria then can with form, half form alive or the inactivation form of living, for example comprise them with the freeze-dried powder form.
The culture supernatant of microorganism also can be included in the compositions.They can be selected from lactobacillus, particularly Lactobacillus and/or Bifidobacterium, and more preferably be selected from Lactobacillus johnsonii (Lactobacillus johnsonii), Lactobacillus reuteri (Lactobacillus reuteri), lactobacillus rhamnosus (Lactobacillus rhamnosus), Lactobacillus paracasei (Lactobacillus paracasei), lactobacillus casei, bifidobacterium bifidum (Bifidobacterium bifidum), short bifidus bacillus (Bifidobacterium breve), animal bifidobacteria (Bifidobacterium animalis), bifidobacteria infantis (Bifidobacterium infantis), bifidobacterium adolescentis (Bifidobacterium dolescentis) and false chainlet bacillus bifidus (Bifidobacterium pseudocatenulatum).According to the most preferred embodiment, used bacterial strain for according to budapest treaty on June 30th, 1992 be preserved in Lactobacillus johnsonii (La1) that Institut Pasteur, preserving number are CNCM1-1225 or according to budapest treaty be preserved in Institut Pasteur on January 12nd, 1999, preserving number is the Lactobacillus paracasei (ST11) of CNCM I-2116.Following compounds can for example be used alone or in combination.Zinc and salt thereof, comprise zinc sulfate and zinc gluconate (zinc glucanate), vitamin B5, B6, B8, C, E or PP, p-carotene and carotenoid, Bulbus Allii extract (particularly diallyl sulfide or Oleum Bulbus Allii form), selenium, curcumin, Curcuminoids, nicotinic acid, alkannic acid (lithospermic acid) and adenosine.Should be understood that the professional can select this type of reactive compound, as they might being united by this way, be suppressed or weaken the intended effect that improves as the compositions of the object of the invention by preventing required targeted activity.
Use
Product of the present invention can be by being used for the treatment of such as the melanic generation of minimizing or preventing cutaneous pigmentation unusually or in beauty treatment to be used for the whitening colour of skin etc. effectively.Really, demonstrate can melanic synthetic (embodiment 1, Fig. 1) in external minimizing for raw coffee bean extract.The generation of tryrosinase also reduces, but degree limited (Fig. 2), this shows that melanic minimizing is not owing to the tyrosine enzymeinhibition, but owing to acts on the mechanism in upstream or the downstream of this enzyme.
Composition of the present invention also has positive role to reinforcing skin barrier and skin moisture-keeping.
Consequently, mottle reduces, and it is brighter more even that the colour of skin becomes, and does not have dyschromasia or dry zone.
Therefore, a theme of the present invention at least a composition that comprises raw coffee bean that relates to effective dose be used for the treatment of as activating agent and/or prevent cutaneous pigmentation unusually, particularly because age or environmental factors UV-induced unusual cosmetic applications for example.
At least a composition that comprises raw coffee bean that the invention still further relates to effective dose is used for brightening or U.S. fair-complexioned cosmetic applications as activating agent, and this is that Aisan wishes especially.
Application of the present invention also comprises at least a at least a activating agent, the particularly applied in any combination of those following activating agents be used to improving moisture content of skin or skin aging that comprises composition and the effective dose of raw coffee bean.
According on the other hand, theme of the present invention is method, the particularly beauty method that treats and/or prevents skin splash and disease, the particularly aesthetic disease relevant with hyperpigmentation in individuality, and the method comprises that at least one uses at least a step that comprises the composition of raw coffee bean of the present invention to described individuality.
Beauty treatment Therapeutic Method of the present invention can assign to carry out by the one-tenth that comprises raw coffee bean at least a of the present invention of Orally administered at least effective dose especially.Orally administeredly comprise once or take in several times Orally administered composition defined above.
It can comprise single application.According to another embodiment, application can repeat for example 2 to 3 times every day, continues one day or more days, and usually continues at least 4 week or 1 to 15 weeks.
In addition, in order to replenish or strengthen the activity of the defined composition of the present invention, it is also conceivable that optional and combined therapy oral or localized forms.
Therefore, the compositions of raw coffee bean that comprises of the present invention can be counted as medicine box with part or the oral medication that the oral or topical composition that randomly contains another kind of active component, particularly probiotic microorganisms or other death, probiotic bacteria that live or half form alive or wetting agent or antidotal agent makes up.Before preparation, each composition is mixed according to order and condition that those skilled in the art are easy to determine.
Certainly, will determine the ratio of coffee bean extract in compositions according to the mode of administration of required effect and compositions.
The daily dose of the raw coffee bean extract of using by oral route can preferably be contained between 0.1 to 27g/ day, depended on product type and content of caffeine, and more preferably 0.5 to 18g/ day, further more preferably 1 to 3g/ day.The inventor finds: according to the effectiveness of raw coffee bean extract of the present invention normally dose dependent and follow dose response curve.If prevent usually gentle skin disorder or damage and will use continually product, then considerably less amount can be enough to realize required effect.If treat serious skin pigment disease, then larger amount can be more suitable, although also can produce effect on a small quantity.
Preferably, expection is used for Orally administered compositions and contains the coffee bean extract of 10% to 80% amount that scope is 1% to 100% and preferred composition weight of composition weight, and this depends on product form.
From following embodiment and accompanying drawing, can obviously find out other advantage of the present invention and feature.Therefore, following examples are used in nonrestrictive mode the field of the invention being described.Except as otherwise noted, percentage ratio in these embodiments is percentage by weight, with " ... extremely ... " numerical range that writes out comprises the upper and lower bound that specifically indicates.
Accompanying drawing
Fig. 1: with the assessment of the melanocytic melanin generation of the pretreated Mus of green coffee extract.
Fig. 2: with the assessment of the melanocytic tryrosinase generation of the pretreated Mus of green coffee extract.
Fig. 3: with the synthetic assessment of silk polyprotein (filaggrin) of the pretreated people of different green coffee extracts epidermal keratinocytes of former generation.
Fig. 4: use the assessment of the hyaluronic acid secretion of the pretreated people of green coffee extract epidermal keratinocytes of former generation.
Fig. 5: the CD44 with the pretreated human epidermic keratinocyte of different green coffee extracts generates.
Embodiment
Embodiment 1:Impact on cutaneous pigmentation
In order to assess composition to skin depigmentation or to promote Pigmented potential beneficial effect, we have carried out with the melanocytic 2D culture of Mus (B16), and 2 test: 1-assessment melanin generate and 2-assessment tryrosinase generates.
1. Cell culture condition
With the B16 cell contain the 1g/L glucose, do not contain among phenol red, as the to be supplemented with 10% hyclone DMEM, in humidification culturing room 37 ℃, contain 5%CO 2Condition under cultivate.
2. Generate melanin by B16 Mus melanocyte system
With cell and selected composition or test object of reference (kojic acid, 400 μ g/mL) together incubation 72 hours under the condition that has or do not exist MSH analog NDP-MSH.Melanic total amount (extracellular is with intracellular) is compared with the melanin standard substance and is existed or do not exist the optical density under the NDP-MSH condition to assess by measure each sample at 405nm.
3. Generate tryrosinase by B16 Mus melanocyte system
With cell and selected composition or test object of reference (kojic acid, 400 μ g/mL) together incubation 48 hours.Generation by immune labeled method assessment tryrosinase.
Composition
We have tested the green coffee extract that removes caffeine of Robusta and Arabica,
Figure BDA00002796736700101
(being provided by Naturex); The green coffee of Oryza ' s Extract-P and water extraction.The test concentrations of every kind of green coffee extract is shown in Table 1.
Figure BDA00002796736700102
Figure BDA00002796736700111
Table 1: green coffee extract is to people's dermal fibroblast of former generation with to the cytotoxicity of people's epidermal keratinocytes of former generation
We have studied the highest no cytotoxicity concentration, and the condition between the different extracts of standardization is further can compare them as far as possible.Therefore, tested the coffee-extract of 40 μ g/mL concentration.
The result
The result represents with the percentage ratio with respect to contrast.Just as expected, test object of reference (kojic acid) has caused the minimizing of black content.Fig. 1 has shown the melanin that generates with selected 72 hours B16 melanocyte of one-tenth divisional processing.
Generally speaking, we have observed coffee-extract the skin plain calmness of discoloring have been had some potentiality, because compare with collating condition, they can reduce the external generation (Fig. 1 and 2) of melanin and tryrosinase.
Embodiment 2:Impact on skin barrier function and water content
The 2D culture of end user's epidermal keratinocytes of former generation is assessed the extract of embodiment 1 to the potential beneficial effect of skin barrier function and moisture content of skin, we carry out 3 test: 1-assessments by the synthetic silk polyprotein of people's epidermal keratinocytes of former generation, and the 2-assessment forms the hyaluronic acid of emiocytosis and the 3-assessment is formed Hemapoiesis by the former representative cornu cutaneum of people matter CD44 (hyaluronic acid receptor) by the former representative cornu cutaneum of people matter.
Cell culture condition
With the human epidermic keratinocyte in restricted (control) keratinocyte SFM culture medium, in humidification culturing room 37 ℃, contain 5%CO 2Condition under cultivate.
By the synthetic silk of human epidermic keratinocyte polyprotein
With cell and the composition of selecting or test object of reference (CaCl 2, 1.5mM) incubation 144 hours together.Generation by immune labeled method assessment silk polyprotein.
Secrete hyaluronic acid by the human epidermic keratinocyte
With cell and the composition of selecting or test object of reference (tretinoin, 10 -7M) incubation 72 hours together.Quantitatively discharged by the hyaluronic acid that the human epidermic keratinocyte in the supernatant causes by the ELISA algoscopy.
Generate CD44 by the human epidermic keratinocyte
With cell and the composition of selecting or test object of reference (tretinoin, 10 -7M) be incubated together 72 hours.Generated by the CD44 that the human epidermic keratinocyte causes by immune labeled method evaluation.
The result
The result is shown in Fig. 3,4 and 5.Green coffee shows that having increased the silk polyprotein generates (Fig. 3).Go the living Robusta of caffeine and arabica coffees extract appropriateness to increase hyaluronic acid secretion (be respectively contrast 128% and 120%) (Fig. 4), hyaluronic acid is a kind of by the endocrine glycosaminoglycans of Skin Cell body, and it helps to keep suitable water content by luring with retain moisture in ECS.Yet the green coffee of Oryza and water extraction has increased the generation (Fig. 5) of (be respectively contrast 148% and 132%) CD44-hyaluronic acid receptor.Stronger skin barrier guarantees to protect better body to avoid the attack of environment and pathogen.It has also limited the loss of moist by epidermis, thereby has guaranteed suitable moisture content of skin.

Claims (13)

  1. At least the raw coffee bean of effective dose or its extract as activating agent for the preparation of the beautifying use that is used for the treatment of and/or prevents the Orally administered composition of skin pigment disease and/or skin defect.
  2. 2. according to each described purposes in the aforementioned claim, it is characterized in that described skin disorder is skin disorder and/or the senile plaque of observing in chloasma, freckle, vitiligo, piebaldism, the phenylketonuria etc.
  3. 3. according to each described purposes in the aforementioned claim, it is used for improving skin color and/or skin complexion.
  4. 4. according to each described purposes in the aforementioned claim, it is used for whitening and/or skin whitening tone.
  5. 5. according to each described purposes in the aforementioned claim, wherein said compositions is further improved the water content of described skin.
  6. 6. according to each described purposes in the aforementioned claim, wherein said compositions is further improved skin barrier function.
  7. 7. according to each described purposes in the aforementioned claim, wherein said extract is derived from coffee bean, and described coffee bean is selected from arabica coffees, Robusta's coffee, middle fruit coffee or large grain coffee (Coffea iberica) kind.
  8. 8. according to each described purposes in the aforementioned claim, wherein said coffee bean is caffeine.
  9. 9. according to each described purposes in the aforementioned claim, wherein said coffee bean extract accounts for 1% to 100% of described compositions gross weight.
  10. 10. according to each described purposes in the aforementioned claim, wherein said compositions is food, beverage, food supplement, nutritional drugs or medicine.
  11. 11. according to each described purposes in the aforementioned claim, wherein said compositions further comprises the food-grade microorganisms of at least a type, particularly probiotic bacteria dead, that live or half form alive.
  12. 12. be used for the treatment of and/or prevent the cosmetic treatment of skin pigment disease and/or skin defect, comprise at least one step from effective dose to individuality that use at least raw coffee bean or its extract of.
  13. 13. be used for the treatment of and/or prevent the test kit of skin pigment disease and/or skin defect, comprise the compositions that contains raw coffee bean at least or its extract with randomly contain death, live or half oral medication of oral or topical composition combination of living the probiotic micro-organisms of form or wetting agent or antidotal agent.
CN201180037623.0A 2010-07-30 2011-07-29 Fresh coffee beans are used for the purposes for adjusting skin pigmentation disorders Expired - Fee Related CN103079525B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10171376 2010-07-30
EP10171376.6 2010-07-30
PCT/EP2011/063088 WO2012013776A2 (en) 2010-07-30 2011-07-29 Use of green coffee beans for regulating skin pigmentation disorders

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CN103079525A true CN103079525A (en) 2013-05-01
CN103079525B CN103079525B (en) 2018-03-09

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EP (1) EP2598102A2 (en)
JP (1) JP2013533282A (en)
CN (1) CN103079525B (en)
BR (1) BR112013002215B1 (en)
MX (1) MX350475B (en)
RU (1) RU2013108968A (en)
WO (1) WO2012013776A2 (en)

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MX2013001078A (en) 2013-03-12
BR112013002215A2 (en) 2016-05-24
CN103079525B (en) 2018-03-09
WO2012013776A3 (en) 2012-03-22
EP2598102A2 (en) 2013-06-05
WO2012013776A2 (en) 2012-02-02
JP2013533282A (en) 2013-08-22
RU2013108968A (en) 2014-09-10
BR112013002215B1 (en) 2017-12-19
MX350475B (en) 2017-09-07

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