JP2006273735A - Harmful metal excretion accelerating activity composition and its manufacturing method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a harmful metal excretion accelerating activity composition and its manufacturing method. <P>SOLUTION: The composition having harmful metal bonding activity or harmful metal excretion accelerating activity has a tea extract containing epicatechin gallate which is extracted from tea leaves with one extracting solvent selected from the group consisting of water, an organic solvent, and a water-containing organic solvent and a cyclodextrin as active ingredients. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a harmful metal excretion promoting active composition and a method for producing the same.

  There are about 54 kinds of minerals in the human body. These are important elements such as bones, teeth, muscles, blood, organs, nerves, and skin, and are present in the living body as ions, and are adjusted so that biological functions work well. Excessive accumulation of harmful minerals in the human body, and lack of nutritional minerals can contribute to a semi-health state and, in turn, can predispose to the onset of illness. Examples of harmful minerals include mercury, lead, cadmium, aluminum, arsenic, nickel, and beryllium. It is known that when these toxic metals are accumulated in the body in large quantities, it is easy to cause a decrease in energy and irritability. In particular, mercury, lead, and cadmium are harmful to the cranial nervous system. In general, harmful metals are easily dissolved in fat, while the brain always needs fat, which is an efficient energy source, so it is considered that harmful metals dissolved in fat reach the cranial nervous system and have various effects on the human body.

  Mercury is classified into inorganic (metal and ionic) and organic mercury compounds, and their toxicity is also different. In the case of metallic mercury, acute poisoning occurs when exposed to high concentrations of mercury vapor, mainly causing bronchitis and interstitial pneumonia, but acute diarrhea and kidney damage may also occur. Chronic poisoning occurs when exposed to low concentrations of mercury vapor repeatedly for a long time, and mainly exhibits symptoms of central nervous system such as fatigue, finger tremor, tendon reflex attenuation, articulation disorder, and memory loss. Inorganic ion-type mercury has a high affinity with thiol groups (SH groups), and binds strongly to the SH groups of cysteine contained in biologically active substances such as enzymes and inhibits its activity. Its toxicity is acute and chronic kidney dysfunction, especially in the proximal tubules.

  Lead is a metal element that has been frequently used in daily life since ancient times, and is widely used as a raw material for storage batteries, alloys and the like in modern society. The initial symptoms of lead poisoning are dry mouth, metallic taste, followed by nausea, abdominal pain, and vomiting. Also included are neurological symptoms such as sensory abnormalities, pain and muscle weakness. Typical symptoms of chronic lead poisoning include lead pallor, anemia, lead limbs, lead colic, extensor paralysis, and coproporphyrin urine. In gastrointestinal symptoms, lead acts on the smooth muscles of the gastrointestinal tract, resulting in loss of appetite, abdominal discomfort, constipation, and abdominal pain. Peripheral neurological symptoms include neuromuscular symptoms, drooping hand due to extensor paralysis of the wrist (lead paralysis) and mild delay of peripheral nerve conduction velocity. The hematological effects of lead are broadly divided into hemolytic anemia and disorders to the heme synthesis system. Hemolytic anemia is mainly caused by impaired uptake of lead transferrin-bound and unbound iron into reticulocytes, impaired pyrimidine 5'-nucleotidase activity, decreased activity of K ion channels and inhibition of sodium pumping of erythrocyte cell membranes it is conceivable that. The disorder of heme synthesis is thought to be mainly due to the inhibition of lead to δ-aminolevulinate dehydrase (δ-ALAD). As a result, urinary δ-ALA and coproporphyrin excretion increases, and blood δ-ALAD Activity is reduced. In addition, the effects of lead on the kidneys, suppression to the immune system, and genotoxicity have also been reported.

  As described above, it is reported that harmful metals accumulate in the human body and thus exert various harmful effects, and development of a method for removing these from the body is anxious. To date, dimercaprol (BAL), penicillamine, 2,3-dimercaptosuccinic acid (DMSA), sodium 2,3-dimercaptopropane-1-sulfonate, formaldehyde sulfone have been used to remove harmful metals from the body. Chelation therapy using metal chelating agents such as sodium xylate is known, but these are generally methods by intramuscular injection or infusion, so they are inconvenient, have large side effects, and are safer and simpler hazardous metals. A removal method is desired.

JP-A-9-141003 JP 2002-114698 A Kostyniak, P.J., J. Toxicol.Environ. Health, 11: 947-957 (1983)

  The present invention has high safety and is ingested as an edible material to adsorb harmful metals such as mercury and lead in the digestive tract (especially in the intestinal tract) and accumulate in the body by suppressing their reabsorption. It aims at providing the composition which has the function which promotes excretion of mercury, lead, etc. which are carried out.

As a result of intensive studies to solve the above problems, the present inventors have found that a tea extract and a mixture of a tea extract and a cyclodextrin bind to a harmful metal to form an insoluble precipitate. The invention has been completed.
That is, the present invention includes the following (1) to (16).
(1) A tea extract containing epigallocatechin gallate extracted from tea leaves with any one extraction solvent selected from the group consisting of water, an organic solvent and a water-containing organic solvent, and containing harmful metal binding activity or A composition having a toxic metal excretion promoting activity.

(2) The tea leaves are Camellia sinensis
sinensis) -derived composition having a harmful metal binding activity or a toxic metal excretion promoting activity according to the above (1).
(3) The composition having noxious metal binding activity or noxious metal excretion promoting activity according to the above (1), wherein the organic solvent is ethanol.
(4) The composition having harmful metal binding activity or harmful metal excretion promoting activity according to the above (1), wherein the harmful metal is mercury or lead.

(5) The composition having a harmful metal binding activity or a toxic metal excretion promoting activity according to the above (1), which is used for a use selected from the group consisting of foods, cosmetics, external medicines, feeds and pharmaceuticals.
(6) A harmful metal adsorbent comprising the composition according to any one of (1) to (5) above.
(7) Extracted from tea leaves with any one extraction solvent selected from the group consisting of water, organic solvents and hydrous organic solvents, tea extract containing epigallocatechin gallate and cyclodextrin as active ingredients, harmful metals A composition having binding activity or harmful metal excretion promoting activity.
(8) The tea leaves are Camellia sinensis.
sinensis) -derived composition having harmful metal binding activity or harmful metal excretion promoting activity according to (7) above.
(9) The composition having noxious metal binding activity or noxious metal excretion promoting activity according to (7) above, wherein the organic solvent is ethanol.

(10) The composition having noxious metal binding activity or noxious metal excretion promoting activity as described in (7) above, wherein the cyclodextrin is β-cyclodextrin.
(11) The composition having noxious metal binding activity or noxious metal excretion promoting activity according to (7) above, wherein the cyclodextrin has a chemically cross-linked structure.
(12) The composition having harmful metal binding activity or harmful metal excretion promoting activity according to (7) above, wherein the harmful metal is mercury or lead.
(13) The composition having a harmful metal binding activity or a toxic metal excretion promoting activity according to the above (7), which is used for a use selected from the group of foods, foods, cosmetics, external medicines, feeds and pharmaceuticals.

(14) A harmful metal adsorbent comprising the composition according to any one of (7) to (13) above.
(15) An aqueous solution containing a tea extract containing epigallocatechin gallate extracted from tea leaves with any one extraction solvent selected from the group consisting of water, an organic solvent and a water-containing organic solvent is mixed with cyclodextrin. A method for producing a composition having a harmful metal binding activity or a harmful metal excretion promoting activity, comprising a drying step.
(16) The process for producing a composition having harmful metal binding activity or harmful metal excretion promoting activity according to the above (15), wherein the cyclodextrin has a chemically crosslinked structure.

According to the present invention, for example, by ingesting orally ingesting harmful metals such as mercury and lead existing in the digestive tract (especially in the intestinal tract) and suppressing reabsorption into the body, mercury accumulated in the body There is provided a composition having a function of promoting the promotion of extracorporeal elimination of harmful metals such as lead.
Hereinafter, the present invention will be described in detail.

  The composition of the present invention is characterized in that it has an adsorption selectivity that adsorbs mercury and lead among harmful metals, while not adsorbing copper as a nutrient mineral. Here, “hazardous metal” refers to a metal that is harmful to the human body by entering the body through the mouth, nose, eyes, skin, and the like. Examples of harmful metals include mercury, lead, cadmium, aluminum, arsenic, nickel, beryllium and the like.

The composition of the present invention comprises a tea leaf extract as an active ingredient and can be produced as follows. The tea leaf used in the present invention is an evergreen tree leaf generally belonging to tea belonging to the genus Camellia belonging to the camellia camellia family in terms of plant taxonomy. More specifically, the leaves of Camellia sinensis (Camellia sinensis), which are cultivated in Japan and China, and the Camellia sinensis (Camellia sinensis) that grows in India and Sri Lanka.
var. assamica; Camellia sinensis var. assamica).

  In the present invention, the tea leaf extract may be used as it is for the extracted tea leaves as they are for extraction, but the tea leaves after passing through the drying step and the roasting step can also be used for the extraction step. The drying step can be carried out according to conventional methods known in the art, such as sun drying, aeration drying in the shade, or heat drying, but is preferably heat drying. By heat drying, the enzyme of tea leaves can be inactivated at the same time, and the change in the component composition of leaves thereafter can be prevented.

  In the heating and drying step, the tea leaves are continuously dried to a target dry state in a range of 40 ° C. or higher and 150 ° C. or lower. The required time varies depending on the state of the tea leaves used in the drying step, but when the fresh leaves are dried, it is at least 2 hours, preferably 4 to 10 hours, more preferably 4 to 8 hours. The temperature is preferably 110 ° C. or lower, and more preferably 80 to 110 ° C. When it is 80 to 110 ° C., a substantially sufficient dry state can be obtained in 4 to 8 hours.

  The roasting process is a process of causing various thermochemical reactions to the dried tea leaves by applying heat. The roasting step preferably includes a roasting step in the first temperature range and a roasting step in the second temperature range. The roasting step is preferably performed on the chopped dried tea leaves. Tea leaves can be shredded manually or mechanically after the drying process, or may be shredded before the drying process. The size of the fine pieces is not particularly limited, but is preferably about 5 mm square. The roasting process can also be carried out continuously following the drying process. For example, the roasting process can be performed while the heat supplied to the tea leaves remains in the drying process. In addition, after cooling in a conditioned atmosphere, the cooled product can be subjected to a roasting process.

  The first temperature range is preferably 100 to 150 ° C. or lower. In this temperature range, the internal moisture remaining on the tea leaves after the drying step can be effectively evaporated. More preferably, it is 100-135 degreeC. Further, from the viewpoint of quickly evaporating the bound water in a short time, the time is preferably from several minutes to a few ten minutes. The second temperature range is a temperature range higher than the first temperature range. By performing the second roasting step after the first roasting step, the temperature range is preferably 180 to 250. More preferably, it is 180-200 degreeC. The roasting time is not particularly limited, but since heating for a long time leads to the disappearance of active ingredients and scents, the time is preferably from several minutes to about ten minutes, more preferably from 2 to 10 minutes. More preferably 3 to 5 minutes. The second roasting step is preferably carried out continuously following the first roasting step. Such continuous operation can effectively achieve removal of internal moisture and disappearance of bitterness components and the like. Although it is preferable to immediately perform the second roasting step on the leaves immediately after the first roasting step, the heat supplied to the hoo leaves in the first roasting step is approximately maintained. The interval between the first roasting step and the second roasting step is included in the continuous implementation.

  In addition to the above, raw tea leaves can be processed into a crude tea form by the following steps and then subjected to an extraction step. First, steamed raw tea leaves are used to inactivate the oxidase in the raw leaves, thereby removing the green odor of the fresh leaves, generating a flavor unique to green tea and increasing the flexibility of the tea leaves. Generally, it is performed at a temperature of 100 ° C. for about 30 seconds. Next, the temperature of the steamed leaves from the steamer is 80 ~ 90 ℃. Since steamed leaves contain almost the same amount of water as fresh leaves, the steamed leaves are cooled to about room temperature so that the water does not evaporate. Next, the steamed leaves are subjected to a rough koji process. The coarse koji process is a process in which the tea leaves are stirred in hot air and softly pressed to make the water in each part of the tea leaves uniform and efficiently dried. This process is roughly divided into a leaf cutting process and a roughing process. The entire roughing process takes about 50 minutes. When steamed leaves are steamed and put into the roughing machine as they are, the leaves will stick together and the roughing process may take time, so it is easy to perform the next roughing process by carrying out the leaf cutting process. To do. Next, in the coarse koji process, the tea leaves are stirred in hot air at about 95 ° C. and pressed to maintain the water evaporation time, and the water content of the tea leaves is reduced to about 1/3. The tea leaves that have undergone the rough koji process are then subjected to a twisting process. This is done for the purpose of making up the water content of the entire tea leaves by making up for the lack of itching in the rough koji process. The twisting process makes it easy to knead and dry the tea leaves after the next mid-straw process. The twisting process varies slightly depending on the state of the tea leaves, but is generally performed at room temperature for about 20 minutes.

  The middle rice bran process is performed to moderately remove the water from the tea leaves that have undergone the twisting process, and to make a medium-boiled tea suitable for the refinement of the next process. The middle rice cake machine applies pressure to the tea leaves with a spatula while stirring in a rotating middle drum, and the water is evaporated by hot air. The mid-boilage process is generally performed for about 40 minutes, and when this process is completed, the weight of the tea leaves is about 1/3 that of fresh leaves. Then, it is subjected to a scouring process. The brewing process is carried out to dry and adjust the shape of the water inside the tea leaves. Generally, the time for this step is about 40 minutes. The tea leaves from the milling machine contain about 13% water. In order to be able to store tea leaves, the drying process is performed by drying with hot air at about 80 ° C. for about 30 minutes so that the moisture content is about 5% using a dryer. The dried tea leaves obtained as described above are crude tea.

  In the above step, the tea leaves used as a raw material can be used uniformly as a whole, or can be separated into a petiole and mesophyll and mixed appropriately. Usually, more components exhibiting the bitterness and pungent taste are contained in the petiole than the mesophyll, and the mesophyll contains a sweet scent component. Therefore, the palatability of the tea leaf extract can be changed by appropriately changing these formulations as necessary.

  The tea leaf extract of this invention can be manufactured by extracting the tea leaf obtained at the said process by a conventional method. The extract manufacturing process is not particularly limited, and various known methods can be employed. For example, the tea leaf extract can be produced by extraction with water and / or a hydrophilic organic solvent (including alcohols such as ethanol and methanol, and ketones such as acetone, preferably ethanol). Usually, extraction is performed with hot water or dilute ethanol (50% by weight ethanol aqueous solution). Hereinafter, an example of the tea leaf extract production process by hot water extraction and alcohol extraction will be described.

  In the case of the hot water tea leaf extract, first, the roasted tea leaf is powdered with a mixer or the like, and about 500 g thereof is weighed and placed in a beaker. Add 500 mL of distilled water and soak for about 30 minutes. Add 500 mL of distilled water and boil on a hot plate or with a burner for about 3 hours. After cooling, filter through a wide-mouth funnel with gauze as a filter cloth. Add 1 L of distilled water to the residue and boil for about 1 hour. After cooling, filter in the same manner. Combine the filtrates and filter again using qualitative filter paper. The obtained filtrate is dried using a freeze dryer, and the dried powder is used as a hot water tea leaf extract.

  In the case of alcohol tea leaf extract, as in the case of hot water tea leaf extract, first, about 500 g of powdered tea leaf is weighed and placed in a beaker. Soak for about 30 minutes in 500 mL of dilute ethanol. Add 500 mL of dilute ethanol, stir well, and leave it for about 24 hours. Filter through qualitative filter paper with funnel. The extract is stored in a refrigerator, and 1 L of diluted ethanol is further added to the residue and left for about 24 hours. In the same manner, it is filtered and dispensed in appropriate amounts into eggplant-shaped flasks and distilled off with an evaporator to obtain an alcoholic tea leaf extract.

  The extract extracted from tea leaves by the above method contains catechins such as epigallocatechin gallate, epigallocatechin, epicatechin gallate, and epicatechin. These components can be easily quantitatively analyzed by a quantitative analysis method known in the art such as liquid chromatography.

  In the present invention, a cyclodextrin is added to the above tea leaf extract to include the leaf extract, whereby a tea leaf extract composition having mercury and lead selective adsorption activity can be produced. Cyclodextrins, also called cyclic oligosaccharides, are α-glucopyranose groups linked in a cyclic form by α-1,4-glycosidic bonds. Depending on the number of pyranose groups, α (6, cyclohexaamylose, empty Pore size = 0.45 nm), β (7, cycloheptaamylose, pore size = 0.70 nm), γ (8, cyclooctaamylose, pore size = 0.85 nm), δ (9, cyclononaamylose) There are four types. As shown in FIG. 1, since the inside of the crown-like structure is hydrophobic and the outside is hydrophilic, a hydrophobic molecule (or a hydrophobic group of a size that can be incorporated into a ring) is formed inside. Inclusion molecules) and inclusion compounds. In the present invention, these can be used alone or in combination of two or more.

  Furthermore, in the present invention, insoluble chemically cross-linked cyclodextrin (cross-linked cyclodextrin) can also be used. Commercially available cross-linked cyclodextrins can be used, but epichlorohydrin, diglycidyl ether, diisocyanates (for example, 1,6-hexamethylene diisocyanate, 1,4-tetramethylene diisocyanate, 1,12-diisocyanate) Using a crosslinking agent such as dodecane, 1,8-diisocyanate octane, 1,5-diisocyanate-2-methylpentane, 1,4-phenylene diisocyanate, etc., cyclodextrin is an organic solvent in which the crosslinking agent can be dissolved (for example, dimethyl) (Sulfoxide, dimethylformamide, hexane, ethyl acetate, tetrahydrofuran, 1,4-dioxane, etc.) can also be prepared by chemical reaction.

  In addition, although a cyclodextrin compounding quantity is not specifically limited, Preferably it is 100-1000 weight part with respect to 100 weight part of tea leaf extracts. More preferably, it is 300-700 weight part, Most preferably, it is 400-600 weight part. Moreover, when blending, various cyclodextrins may be used alone or in combination of two or more.

  The tea leaf extract and / or tea leaf extract inclusion cyclodextrin obtained as described above is in an appropriate form such as powder or aqueous solution depending on the intended use as a harmful metal adsorbent or a harmful metal excretion promoter. Used. It can be added to foods, food additives, cosmetics, external medicines, pharmaceuticals, etc. for the purpose of flexible and safe harmful metal adsorbent or harmful metal excretion promoter. For example, the addition amount of the water-soluble rice bran extract component in foods, food additives, cosmetics, external preparations and the like is usually about 0.01 to 50% by weight, preferably about 0.1 to 2% by weight. The tea leaf extract component is not toxic and is excellent in water solubility, so that no particular problem occurs even if it is added or ingested in a large amount. Moreover, it can also be orally administered as a dietary supplement (supplement), health functional food (nutrition functional food, food for specified health use), and pharmaceutical. The dose in this case is not particularly limited, but for example, about 0.1 to 10 g / day is administered. The additives and excipients required depending on the form of the food or the form of the preparation are well known to those skilled in the art. As a form of food, in addition to making itself a food, it can be dissolved, dissolved or suspended to make a beverage. Moreover, the form added to other foodstuffs can be taken for the purpose of flavor, nutritional supplementation or health promotion. Examples of other foods to be added include confectionery such as rice crackers, buns, and sheepskins, and processed foods such as miso, jam, and sprinkles.

  Although it does not specifically limit as preparation forms, such as a nutritional supplement (supplement), health functional food (nutrition functional food, food for specified health), and a pharmaceutical, It is preferable to set it as an oral agent or an intestinal route agent. Oral preparations include tablets, hard capsules, soft capsules, troches, chewables, powders, granules, fine granules, syrups, solids such as powders and tablets that are dissolved in water when necessary. Can be adopted. The intestinal transit agent can be a liquid or a solid agent such as a powder or a tablet that dissolves at the time of use.

  As long as the effect of the present invention is not hindered, the powder composition of the present invention can contain pharmaceutically or food hygiene acceptable carriers and additives as other components. For example, conventional fillers (for example, starches such as dextrin, saccharides such as lactose, etc.), excipients, lubricants, binders, flavoring agents, flavoring agents, sweeteners and the like can be mentioned.

Examples of the present invention will be specifically described below, but the scope of the present invention is not limited thereto.
[Example 1] Preparation of tea leaf extract Tea leaves obtained by roasting were powdered with a mixer, about 300 g of which was weighed and placed in a 2 L beaker. To this was added 500 mL of diluted ethanol and immersed for about 30 minutes. Furthermore, 500 mL of distilled water was added and boiled on a hot plate for about 3 hours. After cooling, the mixture was filtered with a wide-mouth funnel using gauze as a filter cloth. 1 L of distilled water was added to the residue and boiled for about 1 hour. After cooling, it was similarly filtered. The filtrates were combined and filtered again using qualitative filter paper. The obtained filtrate was dried using a freeze dryer, and the dried powder was used as a tea leaf extract.

[Example 2] Preparation of tea leaf extract inclusion cyclodextrin
In 20 ml of 1M aqueous sodium chloride solution, 0.5 g of crosslinked insoluble β-cyclodextrin and 0.1 g of tea leaf extract were added, and the mixture was shaken for 1 hour at 30 ° C. and then filtered. The obtained filtrate was used for the following metal adsorption test as a tea leaf extract inclusion cyclodextrin. The amount of tea leaf extract included in β-cyclodextrin was measured by measuring the solution before and after filtration by the UV-visible spectrophotometry. As a result, it was found that 0.1638 g of tea leaf extract was included in 1 g of crosslinked β-cyclodextrin.

[Example 3] Metal adsorption test (1) Metal adsorption activity test of tea leaf extract The ability of tea leaf extract to adsorb various metals was examined by examining the ability of the tea leaf extract to form a complex with various metal ions. First, mercury, cadmium, and nickel were prepared to a concentration of about 50 ppm using a physiological saline (0.9% sodium chloride solution) having a pH of 7 to 8, respectively. Further, lead was prepared to a concentration of about 50 ppm using a 0.1 mol / dL sodium nitrate solution as a solvent. Copper was prepared to a concentration of about 50 ppm using a 0.1 mol / dL ammonium nitrate solution as a solvent. 0.01 g of tea leaf extract was added to 15 mL of each solution, and the pH was adjusted to 8.0 using sodium hydroxide. The lead solution was adjusted to pH 7.0 because precipitation occurred at pH 8.0. Subsequently, each solution was filtered with a filter paper, and the filtrate was measured using an atomic absorption photometer. Here, a solution containing no tea leaf extract was set as a blank, and the complex formation rate (%) was evaluated based on the value of the blank solution. As a result, the complex formation rate (%) of tea leaf extract for various metals was 94.5% for mercury, 98.16% for lead, 35.15% for cadmium, 89.37% for nickel, and high complexation with tea leaf extract for metals other than cadmium. It turns out to have the ability.

(2) Metal Adsorption Activity Test of Tea Leaf Extract Inclusion Cyclodextrin First, a metal solution was prepared as shown in Table 1.

  The prepared metal solution was adjusted to any pH using sodium hydroxide, hydrochloric acid, and nitric acid in order to see the effect of pH. To 15 ml of each metal solution was added 0.05 g of the tea leaf extract-encapsulated cyclodextrin prepared in Example 2 (0.005 g only in the case of mercury), and the mixture was shaken in a thermostatic bath at 30 ° C. for 24 hours. Thereafter, the mixture was filtered, and the amount of metal remaining in the filtrate was measured using an atomic absorption spectrophotometry.

  Fig. 2 shows the results of the metal ion adsorption experiment using the tea leaf extract inclusion cyclodextrin. Prior to inclusion, the tea leaf extract formed a complex with any of the metals tested, but by inclusion in β-cyclodextrin, it selectively adsorbs to lead and mercury ions. There was found.

  ADVANTAGE OF THE INVENTION According to this invention, the composition which has the harmful | toxic metal binding activity or harmful | toxic metal excretion promoting activity which can be utilized in field | areas, such as a foodstuff, a foodstuff, cosmetics, an external medicine, feed, a pharmaceutical, can be provided.

FIG. 1 shows the molecular structure of cyclodextrin. FIG. 2 shows the results of adsorption experiments of various metals by the tea leaf extract inclusion cyclodextrin.

Claims (16)

  Extracted from tea leaves with any one extraction solvent selected from the group consisting of water, organic solvents and hydrous organic solvents, tea extract containing epigallocatechin gallate as an active ingredient, harmful metal binding activity or harmful metal excretion A composition having accelerating activity. Tea leaves are Camellia sinensis (Camellia)
The composition having harmful metal binding activity or harmful metal excretion promoting activity according to claim 1, wherein the composition is derived from sinensis).   2. The composition having noxious metal binding activity or noxious metal excretion promoting activity according to claim 1, wherein the organic solvent is ethanol.   2. The composition having harmful metal binding activity or harmful metal excretion promoting activity according to claim 1, wherein the harmful metal is mercury or lead.   The composition having harmful metal binding activity or toxic metal excretion promoting activity according to claim 1, which is used for a use selected from the group of foods, cosmetics, external medicines, feeds and pharmaceuticals.   A harmful metal adsorbent comprising the composition according to claim 1.   Extracted from tea leaves with any one extraction solvent selected from the group consisting of water, organic solvents and hydrous organic solvents, tea extract containing epigallocatechin gallate and cyclodextrin as active ingredients, harmful metal binding activity or A composition having a toxic metal excretion promoting activity. Tea leaves are Camellia sinensis (Camellia)
The composition having harmful metal binding activity or toxic metal excretion promoting activity according to claim 7, wherein the composition is derived from sinensis).   8. The composition having noxious metal binding activity or noxious metal excretion promoting activity according to claim 7, wherein the organic solvent is ethanol.   8. The composition having harmful metal binding activity or harmful metal excretion promoting activity according to claim 7, wherein the cyclodextrin is β-cyclodextrin.   The composition having noxious metal binding activity or noxious metal excretion promoting activity according to claim 7, wherein the cyclodextrin has a chemically cross-linked structure.   8. The composition having harmful metal binding activity or harmful metal excretion promoting activity according to claim 7, wherein the harmful metal is mercury or lead.   8. The composition having harmful metal binding activity or harmful metal excretion promoting activity according to claim 7, which is used for a use selected from the group consisting of foods, foods, cosmetics, external medicines, feeds and pharmaceuticals.   A harmful metal adsorbent comprising the composition according to claim 7.   An aqueous solution containing tea extract containing epigallocatechin gallate is extracted from tea leaves with any one extraction solvent selected from the group consisting of water, organic solvent and hydrous organic solvent, and mixed with cyclodextrin and dried A method for producing a composition having a harmful metal binding activity or a harmful metal excretion promoting activity, comprising a step.   The method for producing a composition having noxious metal binding activity or noxious metal excretion promoting activity according to claim 15, wherein the cyclodextrin has a chemically cross-linked structure.