JP2006258823A - Capillary of capillary electrophoretic analysis system - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To enable viewing without auxiliary lighting especially installed, by checking movement of internal devices in body, and air bubbles infiltrate into gel unit or capillary at short range from observation port to enhance visibility. <P>SOLUTION: This analysis system includes the capillary 66 and its constant-temperature bath 51, optical system 52 neighboring on side 60 of the capillary to irradiate laser beam onto the side, laser head 53 and its laser power source 54 for oscillating the laser beam, pump unit 55 for injecting gel into the capillary, and automatic sampler units 56, 62, 63, 64 arranged on bottom of the constant-temperature bath to supply the capillary with samples, inside the body. It is formed as these above-stated pump unit, optical system and constant-temperature bath are aligned, its body is equipped with a cover containing L-shape section 13, and this L-shape section of the cover comprises a vertical cover section 11 facing the alignment of the pump unit, optical system and constant temperature bath, and a lateral cover section 12 facing the automatic sampler units. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention is an on-line DNA sequencer in which a sample is electrophoresed using a fluorescent primer (a primer to which a fluorescent substance is chemically bonded as a label), and the base sequence is determined by detecting fluorescence from a DNA fragment sample during the electrophoresis. The present invention relates to a capillary electrophoresis analysis apparatus for simultaneously electrophoresis of a plurality of samples using a plurality of capillaries filled with an electrophoresis gel.

  In order to determine the base sequence of a DNA having a long base sequence, a DNA sequencer having high sensitivity, high speed and high throughput is required. As one of the methods, a capillary electrophoresis analyzer in which a plurality of capillaries filled with a gel are arranged instead of using a flat slab gel has been proposed. Capillaries are not only easier to handle and inject samples than slab gels, but also can be migrated at high speed and detected with high sensitivity.

  Capillary electrophoresis analyzers are described in many documents such as Patent Document 1, Patent Document 2, Patent Document 3, and Patent Document 4.

Japanese Patent Laid-Open No. 9-105738 Japanese Patent Laid-Open No. 10-19845 JP-A-10-206384 Special Publication No. 11-502624

  In a conventional capillary electrophoresis analyzer, a space region is formed between a pump unit or an optical system built in a housing and a door provided in the housing, and the space is formed on the door. It is difficult to check the movement of internal devices in the so-called main body from the observation window (also referred to as a confirmation window), air bubbles that have entered the gel unit or capillary, etc., and light does not reach the main body sufficiently. In some cases, it is necessary to make a state in which visual observation can be performed only after installing auxiliary lighting such as internal lighting.

  In view of such points, the present invention improves visibility by checking the movement of internal devices in the main body from the observation window and bubbles entering the gel unit or capillary at a short distance, and in particular, installs auxiliary lighting. It is an object of the present invention to provide a capillary electrophoresis analysis apparatus that can be visually observed even without it.

  A further object of the present invention is to provide a capillary electrophoresis analyzer capable of narrowing the space area between the surface of the pump unit or optical system and the door and easily maintaining the examination environment in a good state.

  Also, conventionally, the capillary body has to be taken out of the box and the operator must carefully set it in the thermostat, and the apparatus requires skill, making the operation difficult and time consuming.

  In view of this point, the present invention eliminates a conventionally configured transport box (package), and can provide a capillary in which a container in which a capillary body is stored can be directly attached to a thermostatic chamber, thereby making work easier. The purpose is to do. It is another object of the present invention to provide a capillary electrophoresis analyzer equipped with a thermostatic chamber in which such a capillary can be incorporated.

  The present invention makes it possible to clearly check the flow of inspection by placing an L-shaped observation window in the center, arranging the pump unit and thermostatic chamber to the capillary in a single line, reducing the space in the warehouse, and equalizing the temperature in the warehouse Enabled stable supply of sample state.

  In addition, the cartridge can be made into a cartridge and the door to the thermostatic chamber can be opened to improve handling.

  Specifically, the present invention provides the following apparatus.

  The present invention relates to a capillary comprising a capillary array, a pipetting section provided on one end side of the capillary array, and a photometry section provided on the other end side of the capillary array, and at least the capillary array is vacuum-sealed on a base plate. Provided is a capillary for a capillary electrophoresis analysis apparatus which is made into a cartridge by pack lamination.

  The present invention provides a capillary array, a pipetting section provided on one end side of the capillary array, and a photometry section provided on the other end side of the capillary array. There is provided a capillary of a capillary electrophoresis analysis apparatus comprising a pair of base plates and capillaries to be formed, wherein the capillary array is packed in the hollow portion to form a cartridge.

  The present invention further provides a capillary in which a protrusion is formed on one base plate, a capillary array is hooked on the protrusion, and a fitting portion of the protrusion is formed on the other base plate so that both base plates are combined. An electrophoresis analyzer capillary is provided.

  The present invention further provides a capillary of a capillary electrophoresis analyzer in which at least two protrusions and two fitting portions are formed.

  The present invention further provides a capillary for a capillary electrophoresis analysis apparatus comprising guide means for attaching the plate member to the capillary electrophoresis analysis apparatus.

  As described above, according to the present invention, the space in the main body is reduced, making it easy to check various devices in the main body, and especially the confirmation of bubbles entering the gel unit or the autosampler unit improves visibility. It became easy to do with it.

  In addition, the operability has been improved along with the improvement in the degree of opening of the thermostatic chamber door.

  Further, the space existing between the cover and the thermostatic chamber can be reduced, and the sample temperature can be made uniform by preventing air convection in this space.

  Further, according to the present invention, since the capillaries are made into a pack type cartridge type, the operability is improved, and it is not necessary to adjust the capillary array one by one, so that the operation is extremely easy. In addition, the vacuum-laminated pack type cartridge is lighter and more simplified.

  An embodiment according to the present invention will be described below with reference to the drawings.

  FIG. 1 shows an overall schematic configuration. In the figure, a capillary electrophoresis apparatus (also referred to as “DNA sequencer apparatus”) is composed of a capillary electrophoresis apparatus main body (hereinafter referred to as “main body”) 1 and a screen input / output processing apparatus 2, both of which are known. Connected by means. These are placed on the base 3.

  The main body 1 includes a cover 4 having an L-shaped part. The L shape is a shape of the main body 1 viewed from the left, and exhibits an inverted L shape when viewed from the right. Here, the “L type” will be described in a unified manner.

  The screen input / output processing device 2 has a central processing unit (CPU) 5, a keyboard 6 and an input device 7 as usual.

  2 is a perspective view of the main body as viewed from the front side, and FIG. 3 is a perspective view of the main body as viewed from the back side. In these drawings, the cover 4 includes a front left cover 11 and a front right cover 12, has an L-shaped portion 13, and includes a lower cover 14 in the vertical direction as an extension thereof. A lower cover 15 is detachably mounted on the lower cover 14 in a continuous manner, and a head cover 16 is provided on the head, a side cover 17 on the side, and a rear cover 18 on the back.

  The front left cover 11 and the front right cover 12 are opened by the door upper handle portions 19 and 20 or the door handle portions 21 and 22, respectively.

  The front left cover 11 is provided with an observation window 31 for viewing a gel unit or the like at a part of the right end thereof in an L shape along the surface of the L shape front left cover 11. An observation window 32 for viewing a pipetting portion or the like is provided in an L shape along the surface of the L shape front right cover 12 at a part of the left end. As will be described later, the observation window 31 is long in the vertical direction to observe (or confirm) the gel unit, and the observation window 32 is long in the horizontal direction.

  From another point of view, the cover 4 includes a vertical surface cover portion 33 and a horizontal surface cover portion 34. Accordingly, from another point of view, the L-shaped portion of the cover is formed by the vertical surface cover portion 33 and the horizontal surface cover portion 34, and extends over the vertical surface cover portion 33 and the horizontal surface cover portion 35 as described above. Two observation windows 31 and 32 are provided. The lateral surface means not only horizontal but also an inclined surface.

  The lower cover 15 is provided with a power indicator 37 and a power switch 38. The rear cover 18 is provided with an exhaust fan 39, a main power switch 40, a power source and a communication cable hole 41 as in a normal device, and a lid 42 is provided at a part thereof. FIG. 4 shows various devices and arrangements arranged inside the main body, and details are omitted. In the figure, inside the main body, a capillary (described later), its thermostat (oven) 51, an optical system (optical device) 52 that irradiates the photometric unit with laser light, adjacent to the photometric unit (described later) of the capillary, laser light An oscillating laser head 53, its laser power supply 54, a pump unit 55 for injecting gel into the capillary, and an autosampler 56 for supplying a sample to the capillary are provided. The optical system 52 is shown as a block, but details are shown in FIG. A substrate 57 is provided on the side surface of the thermostatic chamber 51. The thermostatic chamber 51 is a device for keeping the capillary 66 at a constant temperature during electrophoresis. A power supply unit 59 is disposed behind the thermostatic chamber 51.

  Note that FIG. 4 is for explaining a rough arrangement inside the main body, and the optical system 52 is a general term for the lens, filter, shutter, optical sensor, etc. described in FIG.

  In such an apparatus configuration, the pump unit 55, the optical system 52, and the constant temperature unit 51 are arranged in a row. The constant temperature part 51 has a rectangular parallelepiped shape, but its longitudinal direction coincides with the one-row arrangement direction. In such an arrangement, these devices are disposed on the pedestal 58, but no device is disposed on the front portion of the pedestal 57. An autosampler 56 is provided at a position on the right side with the pedestal 57 as described later.

  FIG. 5 shows the inside of the main body with the door of the thermostatic chamber 51 opened, FIG. 6 shows an example of a capillary used, and FIG. 7 shows a state where the door of the thermostatic chamber 51, that is, the thermostatic chamber door 61 is closed. The inside of the main body is shown.

  In these drawings, the thermostatic chamber 51, the optical system 52, and the pump unit 55 are arranged in a horizontal row and are drawn backward from the pedestal 58, that is, arranged so as to allow the installation of the cover 4 having the L-shaped portion. Established. An autosampler 56 is disposed below the thermostatic chamber 51. As shown in FIG. 7, the autosampler 56 is provided with an autosampler XYZ shaft 62, and is movable in the X, Y, and Z3 axis directions by driving a motor.

  On the auto sampler 56, a sample tray 63 and a buffer tank 64 are placed to constitute an auto sampler unit. Therefore, the sample tray 63 and the buffer tank 64 can be moved in the X, Y, and Z3 axis directions by the auto sampler 56. A buffer solution (buffer solution) is stored in the buffer tank 64.

  The sample tray 63 and the buffer tank 64 are arranged in a horizontal plane, moved in the horizontal depth direction (X direction) to be positioned on the pipetting section 68 of the capillary array 67, and brought into contact with or separated from the buffer liquid. To move in the vertical direction (Z direction). Further, in order to move below the buffer tray 65, the movement in the horizontal direction (Y direction) is performed.

  A voltage for sample injection is applied to the pipetting unit 68 from the lower electrode (not shown) through the buffer solution. A platinum electrode for this is provided. The sample tray 63 is provided with 48 sample containers, for example.

  The constant temperature bath 51 is provided with a projection 65 for providing a capillary, and a capillary is provided on this. An example of the capillary 66 is shown in FIG. As shown in the drawing, the capillary 66 is composed of 16 capillary arrays 67 as an example, a pipetting section 68 provided on one end side of the capillary array 67, and a photometry section 69 provided on the other end side. The The capillary 66 having such a configuration is installed in the thermostatic chamber 51. In the figure, the pipetting unit 68 is positioned below and the photometry unit 69 is positioned on the left side.

  The pump unit 55 is arranged in parallel so as to face the constant temperature bath 51, and the optical system 52 is arranged in parallel between the constant temperature bath 51 and the pump unit 55.

  A syringe and capillary, which will be described later, are attached between the thermostatic chamber 51 and the pump unit 55, and a gel block 70 in which a passage communicating with each other is formed, and a gel injection syringe 71 for injecting a gel into the capillary 66 via the gel block 70. A reservoir syringe 72 for replenishing the gel to the gel injection syringe 71 is disposed, a buffer jar 73 is disposed below the gel block 70, and a buffer jar tray 74 is disposed below the buffer jar 73. A buffer solution is stored in the buffer jar 73, and an electrode is inserted into the buffer solution, and becomes a ground potential during electrophoresis.

  The gel 66 is filled into the capillary 66 from the gel injection cylinder 71 through the gel block 70. That is, although the gel-filled capillary 66 is used as an analysis unit, the pipetting unit 68 is put in a buffer solution, and the eluted DNA fragment is irradiated with light to detect fluorescence. A sheath flow (buffer solution flow) is formed with a buffer solution so that DNA fragments eluted from the gel do not spread and decrease the separation performance.

  FIG. 8 is a view of the inside of the main body as viewed from the right side.

  From the side surface direction (Y direction in the figure) of the photometry unit 69, the laser light emitted from the lasers 75 and 76 is collimated into a parallel light beam by a lens and then irradiated to excite the separated sample in the photometry unit 69. To do. A shutter is provided between the lasers 75 and 76 and the lens so that the sample can be selectively excited. The fluorescence generated by the laser light irradiation is extracted from the X-axis direction orthogonal to the Y-axis direction, collected by the condensing lens 82, light having a wavelength to be detected by the filter 84, and the imaging lens 86. Thus, an image is formed on an optical sensor 88 such as a two-dimensional CCD sensor. The signal detected by the optical sensor 88 is sent to the screen input / output processing device 2 which is a signal processing device, subjected to signal processing, the type of the terminal base is identified based on the fluorescence wavelength, and the acid sample is detected based on the measurement signal. Is analyzed.

  In determining the base sequence of DNA (deoxyribonucleic acid), generally, four wavelengths are measured. A fluorescent dye is bound in advance by a reaction operation such that various large wavelengths correspond to the types of bases at the ends of the DNA fragments.

  The buffer solution flows through the photometry unit 69, flows into the buffer jar 73, and is stored in the buffer tank via the buffer jar tray 74.

  In FIG. 5, the control box 80 includes a control board (not shown), and various information for measurement is input from the CD insertion slot 81 by the CD. The control box 80 is a CPU, an A / D converter that converts a detection signal from the photomultiplier tube into a digital signal and imports it into the CPU, and transfers signals to and from each unit in accordance with instructions from the CPU. A temperature controller that controls the temperature of the interface and the thermostatic chamber 51 is provided. The I / Φ interface includes a temperature controller, a power supply for the pump unit 55, a high-voltage power supply for sample injection and electrophoresis, a sensor and a motor for the scanning mechanism, a sensor and a motor for the autosampler XYZ shaft 62, a laser device (laser head 53, laser power supply) 54), connected to an interlock safety switch, an indicator light, and the like. The CPU is connected to the screen input / output processing device 2 shown in FIG. 1 to perform data processing.

  After sample injection, the autosampler XYZ shaft 62 is operated, the end of the capillary array 67 of the pipetting unit 68 is immersed in the sample tray 63, and high voltage is applied to the electrodes to start migration. During the electrophoresis, the constant temperature chamber 51 is controlled at a constant temperature so as not to cause compression.

  When the electrophoretic-separated DN fragment passes through the photometry unit 69, the optical system 52 is scanned to detect fluorescence from the fluorescent substance that labels the sample. At this time, the fluorescent label is discriminated based on the intensity ratio of the detected fluorescence signal, and the base is identified. The vertical surface cover portion 33 of the cover 4 is disposed in the vertical direction, and the auto sampler 56 is positioned in the vertical direction. The space region 91 between the vertical surface cover 33 and the thermostatic chamber 51 is formed into a very narrow shape by adopting an L shape. The autosampler 56 is accommodated in the area of the autosampler chamber 92. The volume of the space area 91 is smaller than that of the area of the autosampler chamber 92, and the air convection accompanying the temperature rise is It occurs mainly in the area of the sampler chamber 92 and has little influence on the space area 91. In addition, since the convection generated in the region of the autosampler chamber 92 is small, the flow of heat is very small, the temperature difference in the main body is uniformed, and a stable and good state of the sample is obtained as data. It will be.

  Conventionally, a space area was formed between the door formed on the housing and the pump unit or optical system, and this space area caused convection in the cabinet, and the inspection environment was unstable. In addition, by forming the space area between the surface of the optical system and the door so as to be narrow, a capillary electrophoresis analyzer that can easily maintain the examination environment in a good state is provided.

  FIG. 9 shows the appearance of the cover 4. As shown in the figure, an L-shaped gel unit observation window 31 and a pipetting observation window 32 are formed in the L-shaped portion of the cover 4 as described above. The gel unit observation window 31 is formed of a vertical observation window portion 93 and a planar observation window portion 94. The pipetting observation window 32 is formed of an observation window portion 95 and an observation window portion 94.

  The cover 4 includes a lower cover 14 that is continuous with the horizontal surface cover portion 34 and extends in a direction opposite to the vertical surface cover portion 33, and the L-shaped observation window portion 93 ′ extends to a part of the lower cover 14. The cover 4 is formed of a front cover part 11 and a front right cover part 12 which are opened in the left-right direction, and an observation window part 93 ′ formed so as to extend is provided in the front left cover part 11 and is a gel unit. A first observation window portion 94 that monitors the state of bubbles entering into the front surface, and a second observation window portion 95 that is provided on the front right cover portion 12 and that monitors the state of bubbles entering the sample flowing from the autosampler to the capillary. It consists of. The first observation window portion 94 and the second observation window portion 95 are provided at the right end of the front left cover portion 11 and at the left end of the front right cover portion 12, respectively, so that they form one observation window. Thus, a series of flow from the auto sample to the sample suction and the gel injection can be continuously observed, and visibility is ensured.

  As described above, the main body includes the cover 4 having the L-shaped portion, and the L-shaped portion of the cover 4 includes the vertical surface cover portion 33 and the horizontal surface cover portion 34 facing the auto sampler 56, The surface cover portion 33 and the horizontal surface cover portion 34 are provided with L-shaped observation window portions 93 and 93 ′ so that the vertical direction and the horizontal direction in the main body can be visually recognized.

  From the first observation window portion 94, the gel block 70, which is a gel unit, the gel injection syringe 71 and the reservoir syringe 72, the buffer jar 73, and the buffer jar tray 74 can be observed, and from the second observation window portion 95, It is possible to observe the movement of the sample tray 63 and the buffer tank 64 in the X, Y, and Z axis directions, and the suction state of the sample in the pipetting unit 68. As described above, since the first observation window portion 94 and the second observation window portion 95 are continuously integrated, the sample tray 63 and the buffer tank 64 extend beyond the front left cover 11 and the front right cover 12 to Y. Useful for observing moving in a direction. In addition, the air bubbles entering the gel unit and capillary 66 can be observed and confirmed. Note that the second observation window portion 95 is sufficient if only a part of the pipetting portion 68 and the capillary array 67 are observed, and is not provided on the cover corresponding to the thermostatic chamber 51. Therefore, the second observation window 95 is lower in height than the first observation window 94, but has a long shape in the Y direction for easy observation in the Y direction. FIG. 10 is a cross-sectional view of FIG. 9 viewed from the lateral direction. FIG. 11 shows details of the observation window portion 93. The L-shaped observation window portion 93 formed in the L-shaped portion is made of a transparent plate 96 on the outer surface and a metal mesh material 97 on the inner surface. These are attached to the iron plate 98 of the cover. The metal mesh material 97 and the iron plate 98 shut off electromagnetic waves. Even in this case, the transparency is ensured.

  As shown in FIG. 10, the X, Y, and Z axes in the vertical arrangement of the gel units such as the optical system 52 and the gel block 70, and the horizontal arrangement of the auto sampler units such as the auto sampler 56, the sample tray 63, and the buffer tank 64. The device moving in the direction can be immediately seen through the observation window portion 93 provided in the vertical surface cover portion 33 and the horizontal surface cover portion 34, and visibility is ensured.

  In this way, the distance from the observation window 93 to the optical system 52, gel unit 71, etc., or the sample tray 63, buffer tray 64 inside the main body is reduced, and the movement of the internal mechanism and the confirmation of bubbles entering the gel unit 71 and the capillary are confirmed. Is close enough, and the light reaches sufficiently, so that sufficient visual observation is possible without other auxiliary light.

  FIG. 12 shows a situation where the front left cover 11 and the front right cover 12 are fully opened as indicated by the dotted line 100, and the door (thermostatic chamber door) 61 of the thermostatic chamber 51 is opened.

  As described above, the pump unit 55, the optical system 52, and the thermostatic chamber 51 (capillary 66) are arranged in a row so as to reduce the space in the main body and to open the front left cover 11 and the front right cover 12 over the entire surface. Thus, the operability is improved by allowing the operating range of the hand 102 of the operator 101 to reach the entire apparatus.

  The cover 4 includes a front left cover 11 and a front right cover 12, and the front left cover and the front right cover are opened by hinges provided on the main body, respectively, and the pump unit 55, the optical system 52, and the thermostat arranged in the row. An opening 103 is formed to take out any of the tanks 51 in the forward direction (operation direction).

  As described above, the cover 4 is formed so as to have the L-shaped portion, and the L-shaped observation window 93 is provided on the cover 4, thereby providing the following configuration and obtaining the effect. It was.

  Inside the main body, the capillary and its thermostat, adjacent to the measurement of the capillary, an optical system for irradiating the measurement with laser light, a laser head for oscillating the laser light and its laser power supply, a pump unit for injecting gel into the capillary, and An autosampler unit is provided in the lower part of the thermostat and supplies a sample to the capillary. The pump unit, the optical system, and the thermostat are arranged in a line. The main body has an L-shaped part. The L-shaped part of the cover is composed of a vertical cover part and a horizontal cover part facing the autosampler unit, and the vertical cover part and the horizontal cover part have an L-shaped shape. There is provided a capillary electrophoresis apparatus that is provided with an observation window to visually recognize the vertical direction and the horizontal direction in the main body.

○ Leveraging the characteristics of the L-shaped arrangement, the pump unit to the thermostatic chamber are arranged in a row to minimize the space in the main body and maximize the opening of the thermostatic bath (improvement after opening) to improve operability. It was.

○ The status of the inside of the main unit can be checked with ease, and the movement of the internal mechanism and the confirmation of air bubbles entering the gel unit or autosampler unit can be seen at a short distance without the need for internal lighting.

○ In contrast to the conventional method that requires independent confirmation windows for both the gel unit and the autosampler, an L-shaped observation window that is continuous only to the main confirmation part is moved closer to the device along the flow of operation and sample. In addition, the confirmation work that previously required peeping has been improved.

○ Stable inspection data can be obtained by reducing the wasteful space as much as possible and eliminating the accumulation of heat around the auto-sampler unit in the cabinet to make the sample temperature uniform.

○ When a conventional observation window was provided, the electromagnetic wave data was affected. A metal mesh was laminated inside the transparent window to enable electromagnetic shielding, and a see-through condition was created to allow confirmation of the interior. In addition, the inspection flow can be clearly confirmed.

  Next, FIG. 13 shows an example of a packaged capillary. In the figure, a capillary 66 comprises a capillary array 67, a pipetting portion 68 provided at one end thereof, and a photometric portion 69 provided at the other end thereof, and at least the capillary array 67 is formed into a cartridge as a vacuum pack laminate 112 on a base plate 111. The heat-resistant pack cartridge capillary is used. And the pipetting part 68 is located in the lower part of the base plate 111, and the photometry part 69 is located in the side part. The tip of the capillary array 67 is immersed in the buffer tank 64. Guide holes 113 to be attached to the thermostatic chamber 51 are provided at the four corners of the base plate 111, and hooks for attaching the capillary 66 to the thermostatic chamber 51 are also provided and can be easily attached.

  In FIG. 14, at least a capillary array 67 is formed into a cartridge as a vacuum laminate 112 on a base plate 111, a photometry unit 69 is positioned on the top of the base plate 111, and a pipetting unit 68 is positioned on the bottom, and the capillary array is linearly moved up and down. An example of arrangement is shown. The base plate 111 can be made transparent to be transparently packed. According to this configuration, the inside can be confirmed. The thermostatic chamber 51 is set to about 50 ° C., and the capillary is required to withstand this temperature. The ability to withstand such temperatures is referred to herein as heat resistance.

  FIG. 15 shows a heat-resistant pack cartridge capillary 66 that is similar to the configuration of FIG. 13 but is formed into a cartridge as a vacuum pack laminate 112 by multilayering a capillary array 67 with a laminate material interposed.

  FIG. 16 shows an example in which the heat-resistant pack cartridge capillary 66 shown in FIG. 15 is set in the constant temperature bath 51 in the apparatus. A guide hole 113 is fitted into a hook 114 provided in the thermostatic chamber 51, and the heat-resistant pack cartridge capillary 66 is attached. Of course, the heat-resistant pack cartridge capillary shown in FIGS. 13 and 14 can also be mounted.

  According to this configuration, inside the main body, the capillary, its thermostat, adjacent to the measurement of the capillary, an optical system that irradiates the measurement with laser light, a laser head that oscillates the laser light, its laser power supply, and a gel on the capillary A heat-resistant pack cartridge capillary that is provided in a lower part of a constant temperature bath and an autosampler that supplies a sample to the capillary and includes a sample tray and packaged in the capillary is used. There is provided a capillary electrophoresis analysis device mounted in a packaged state in a tank.

  In addition, a capillary electrophoretic analyzer is provided in which the heat-resistant pack cartridge capillary has a guide hole for attaching to the thermostat, and the thermostat is provided with a hook for attaching to the main body.

  In this case, a capillary electrophoresis analyzer having a configuration in which a pump unit, an optical system, and a thermostatic chamber are arranged in a row is provided.

  FIG. 17 shows a hollow pack type heat-resistant pack cartridge capillary. The capillary 66 includes a capillary array 67, a pipetting portion 68 provided on one end side thereof, and a measurement 69 provided on the other end side, and the capillary array 67 can be combined. The two base plates 121 and 122 that form a hollow inside are packed in the hollow 123 and formed into a cartridge to form a heat-resistant pack cartridge capillary. The base plate 121 is provided with a protrusion 124, and the base plate 122 is provided with a fixing hole 125 into which the protrusion 124 is fitted. When the base plate 122 is closed as indicated by an arrow, the protrusion 124 and the fixing hole 125 are united and integrated. At least two fitting portions such as the protruding portion 124 and the fixing hole 125 are formed, a protruding portion is formed on one base plate, a capillary array is hooked on the protruding portion, and the protruding portion is connected to the other base plate. The heat-resistant pack cartridge capillary is provided by combining both base plates.

FIG. 18 shows a hollow pack-type capillary 66 provided with a photometric part 69 on the side, and the capillary array 67 is (a) case 200 to 360 mm, (b) case 500 mm,
(C) An example in which the case is 800 to 900 mm is shown. The arrangement of the two protrusions 124 and 124 ′ is changed depending on the length of the capillary array 67.

  FIG. 19 shows an example in which the heat-resistant pack cartridge capillary 66 of FIG. Other configurations are the same as the example shown in FIG. According to cartridge type capillaries, conventionally, the capillary body had to be taken out of the box and the operator had to set it carefully in the oven. According to the capillary according to the invention, the following effects are achieved.

○ Capillary body is pack-type and can be easily installed in the oven.
○ Guide holes for attaching to the oven are provided at the four corners of each pack, and there are hooks for attaching it to the oven, making it easy to install as a guide.
○ There are 4 types of lengths in the capillary body depending on the number of examinations and the number of specimens. Guide hooks corresponding to the lengths are provided in two locations in the package, and adjustments and measures to prevent the capillaries from being exposed inside and reinforcement of the strength of the entire package, etc. The function as a material can be given.

The system block diagram of the Example of this invention. The front side perspective view of the Example of this invention. The back side perspective view of the example of the present invention. Arrangement configuration diagram. The apparatus internal arrangement | sequence figure in the state which opened the door of the thermostat. The figure which shows an example of a capillary. The apparatus internal arrangement | sequence figure in the state which closed the door of the thermostat. The side sectional view of the example of the present invention. The perspective view which shows an observation window structure. The figure for demonstrating ensuring of visibility. FIG. 11 is a partial detail view of FIG. 10. The figure for demonstrating operativity. The perspective view which shows one example of the capillary of the Example of this invention. The perspective view which shows the example of another capillary. The perspective view which shows the example of another capillary. The figure which shows the example which incorporates a capillary in a thermostat. FIG. 5 is a perspective view showing another example of the capillary, (A) is a diagram showing an example in which a capillary is mounted on one side, and (B) is a diagram for forming a hollow pack that can be opened and closed. The figure which shows the modification of a hollow pack cartridge capillary in the case of (A) (B) (C). FIG. 18C is a diagram illustrating an example in which the case is incorporated in a thermostatic chamber.

Explanation of symbols

  DESCRIPTION OF SYMBOLS 1 ... Capillary electrophoresis main-body part (main body), 2 ... Screen input / output processing apparatus, 4 ... Cover, 11 ... Front left cover, 12 ... Front right cover, 13 ... L-shaped part, 14 ... Lower cover, 15 ... Bottom Cover: 16 ... Head cover, 17 ... Side cover, 18 ... Rear cover, 31, 32 ... Observation window, 33 ... Vertical cover, 34 ... Horizontal cover, 51 ... Constant temperature bath (oven), 52 ... Optical system 53 ... Laser head 54 ... Laser power source 55 ... Pump unit 56 ... Auto sampler 61 ... Constant temperature chamber door 62 ... Auto sampler XYZ axis 63 ... Sample tray 64 ... Buffer tank 66 ... Capillary 67 ... capillary array, 68 ... pipetting section, 69 ... photometry section, 70 ... gel block, 71 ... gel injection syringe, 72 ... reservoir syringe, 73 ... buffer jar 74: Buffer jar tray, 77 ... Hollow capillary, 80 ... Control box, 112 ... Vacuum pack laminate, 113 ... Guide hole, 114 ... Hook, 121, 122 ... Base plate, 123 ... Hollow, 124 ... Projection, 125 ... Fixing hole .

Claims (5)

In a capillary comprising a capillary array, a pipetting part provided on one end side of the capillary array, and a photometric part provided on the other end side of the capillary array,
A capillary of a capillary electrophoresis analyzer characterized in that at least the capillary array is vacuum packed and formed into a cartridge on a base plate. In a capillary comprising a capillary array, a pipetting part provided on one end side of the capillary array, and a photometric part provided on the other end side of the capillary array,
A capillary of a capillary electrophoresis analyzer comprising a pair of a base plate and a capillary that form a hollow part when combined, and the capillary array packed into the hollow part into a cartridge. The capillary according to claim 2,
A projection is formed on one base plate, a capillary array is hooked on the projection, and a fitting portion of the projection is formed on the other base plate to combine the two base plates. Electrophoresis analyzer capillary. The capillary according to claim 3,
The capillary of the capillary electrophoresis analyzer is characterized in that at least two protrusions and two fitting parts are formed. The capillary according to claim 1 or 4,
A capillary for a capillary electrophoresis analyzer, comprising a guide means for attaching the plate material to the capillary electrophoresis analyzer.

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