JPH11326276A - Electrophoretic analyzer and analytical method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an electrophoretic analyzer whose structure and constitution are compact, which is handled easily and by which an analytical operation is performed easily and to provide an analytical method which is used easily. SOLUTION: An electrophoretic analyzer is provided with a pedestal 90 which is constituted of a longitudinal plate part 91 and a plane plate part 92. A sheath flow cell base 96 which is equipped with a sheath flow cell 60, capillaries 40 which are combined with the sheath flow cell 60, a hot plate 94 which controls the temperature of the capilalries 40 and a laser irradiation hole member 93 by which the sheath flow cell 60 is irradiated with laser light constitute a group which is placed on the longitudinal plate part 91 at the pedestal 90. A buffer-solution tank 32 and a sample tray 100 in which a sample is housed are placed on a table. The table is installed on the plane plate part 92 at the pedestal 90, The group and the table are arranged in a sample chamber which is formed in an analyzer body together with the pedestal 90.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to an electrophoresis analyzer and an analysis method. In particular, the present invention provides a DNA sequencer (D) for analyzing a biological sample such as DNA (deoxyribonucleic acid) using a plurality of capillaries or microchannels as a migration medium.
The present invention relates to an electrophoresis apparatus suitable for use in an NA base sequence analysis apparatus) and an analysis method.

[0002]

2. Description of the Related Art DNA analysis technology based on electrophoresis,
In particular, DNA sequencers (DNA base sequence analyzers)
However, it has become widespread. As the need for analysis increases, there is an increasing need to improve the throughput of analysis. One method of improving the analytical throughput is to integrate an electrophoretic medium.

Japanese Patent Application Laid-Open No. Hei 6-138037 discloses an electrophoresis apparatus in which a sample separation section provided between a cathode electrode tank and an anode electrode tank to which a voltage is applied by a power supply is a migration path composed of a capillary. The other end of the capillary pair, one end of which is connected to the cathode electrode tank or the anode electrode tank, respectively, is arranged in the optical cell so that their axes are substantially aligned and opposed to each other with a fixed gap therebetween and penetrates the optical cell. Migration path, and a sheath liquid container provided outside the optical cell,
A sheath liquid is injected from the sheath liquid container into the optical cell, and the sample is electrophoresed from the end of the capillary for electrophoresis separation, which is the upstream electrophoresis section, which is the sample separation section, and guided to the opposing capillary. Sheath flow forming means, and light detecting means for detecting the sample by irradiating light from a light source to the optical detecting unit with the gap portion as an optical detecting unit,
A plurality of optical detectors formed by a plurality of capillary pairs are arranged in the optical cell, the plurality of optical detectors are connected to each other via an electrolyte, and the plurality of optical detectors are located on a straight line The plurality of capillary pairs are aligned and arranged so that the excitation light is irradiated along the straight line so as to simultaneously irradiate all the optical detection units, and the fluorescence emitted from the sample that migrates and flows out of each capillary is simultaneously emitted. An electrophoresis device for detection is described.

[0004] The second system is disclosed in US Pat.
As described in JP-A-92412 and JP-A-5-93711, a multi-channel system in which fine grooves are formed on the surface of a glass plate and used as migration paths has also been proposed. Also in this method, a large number of samples can be simultaneously separated and analyzed at high speed.

In the above-described multi-channel system, first, an electrode and one end of a capillary are inserted into a sample accommodated in a sample container, and then a voltage is applied to both ends of the capillary to electrically sample the sample. Move and introduce into the capillary, then into the buffer solution in the buffer tank,
After inserting the electrode and one end of the capillary, a voltage is applied to both ends of the capillary, and the sample is separated by electrophoresis.

[0006]

If the number of samples to be analyzed at the same time is increased to several tens in order to improve the analysis throughput, it becomes difficult to insert electrodes and capillaries.

According to the present invention, there is provided an electrophoretic analyzer and an analysis method which are easy to use by making the structure and configuration of the apparatus compact and easy to handle in using the multi-capillary system. The purpose is to:

[0008]

According to the present invention, a capillary and a sheath flow cell to be combined with the capillary are arranged in a vertical direction, a sample plate on which a sample is placed is arranged in a planar direction, and a buffer liquid tank is provided at a line where the two arrays intersect. Place,
The two are joined via the liquid in the buffer liquid tank. Each device group constituting the two arrangements was placed on a pedestal comprising a vertical plate portion and a flat plate portion, so that the devices could be easily assembled and used. Further, the present invention distinguishes between a frequently used device and a less frequently used device in a manner of mounting on a pedestal, and a frequently used device such as a combination of a capillary and a sheath flow cell or a sample plate is placed on the pedestal. It was placed without being fixed.

In addition, the present invention adopts such an apparatus structure so that an improved pipetting method can be adopted without changing an existing pipette.

The present invention specifically provides the following apparatus and method.

According to the present invention, there is provided an electrophoresis analyzer for optically electrophoretically analyzing a plurality of sample components, comprising a pedestal comprising a vertical plate portion and a flat plate portion, and having a sheath flow cell stage. A group consisting of a capillary combined with the sheath flow cell, a hot plate for controlling the temperature of the capillary, and a laser irradiation port member for irradiating the sheath flow cell with laser light is mounted on the vertical plate portion of the pedestal, and a buffer solution is provided. A sample tray containing a tank and a sample is placed on a table, the table is mounted on a flat plate portion of the pedestal, and the group and the table mounted as described above are formed together with the pedestal on an analyzer main body. The present invention provides an electrophoresis analyzer arranged in a sample chamber.

The present invention relates to an electrophoresis analyzer for optically electrophoretically analyzing a plurality of sample components, a sheath flow cell, a capillary combined with the sheath flow cell, a hot plate unit for controlling the temperature of the capillary, and the sheath. A group of laser irradiation port members for irradiating a laser beam on the flow cell is arranged on a vertical surface, a plurality of sample trays having sample containers arranged in parallel are arranged on a plane, and both are arranged. Provided is an electrophoresis analyzer in which a buffer solution tank is disposed at a line where two surfaces intersect, and the tip of the capillary is immersed in a solution in the buffer solution tank.

According to the present invention, there is provided an electrophoresis analyzer for optically electrophoretically analyzing a plurality of sample components, comprising a pedestal comprising a vertical plate portion and a flat plate portion, wherein a laser beam is provided on the vertical plate portion of the pedestal. A laser irradiation port member for irradiating light and a hot plate for performing temperature control are fixed, and a combined body of a sheath flow cell and a capillary is irradiated with a laser beam to the sheath flow cell so that the hot plate heats the capillary. The sample plate is mounted without being fixed to the vertical plate portion, a plurality of sample trays are fixed on a tray holder, the tray holder and the buffer liquid tank are mounted without being fixed on a table, and the table is mounted on an analyzer. Provided is an electrophoresis analyzer which can be slid and mounted without being fixed to a holding section of a sample chamber formed in a main body.

According to the present invention, in a electrophoretic analyzer for optically electrophoretically analyzing a plurality of sample components, a box housing a laser emitting a laser beam, a sample storage chamber for storing a sample, and a sample are analyzed. It comprises a box-shaped DNA sequencer main body composed of a sample chamber, and a screen input / output device.The sample chamber has a sheath flow cell, a capillary combined with the sheath flow cell, and temperature control of the capillary. A unit with a hot plate and a laser irradiation port for irradiating the sheath flow cell with laser light, a group arranged in the vertical direction, and a sample tray containing a buffer solution tank and a DNA sample, Another group arranged in the direction is arranged, the opening and closing door of the sample chamber can be slid in the vertical direction Providing an electrophoretic analyzer are disposed.

According to the present invention, there is provided an electrophoretic analysis method for optically electrophoretically analyzing a plurality of sample components, wherein a sample is prepared using a microtiter plate, and a pitch P between adjacent sample containers is determined by the microtiter plate. A sample tray having an integer fraction of the pitch of the sample is prepared, the pitch of a pipette for pipetting a sample prepared from the microtiter plate is made equal to the pipette of the microtiter plate, and the sample is sampled from the microtiter plate. When pipetting a sample into a container,
The sample is divided and pipetted by setting the number of the denominator to an integral number, and the pipette sample is pipetted to a sheath flow cell arranged in a vertical direction in parallel with the sample tray and a capillary combined with the sheath flow cell. An electrophoretic analysis method for performing analysis by inhalation is provided.

[0016]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment according to the present invention will be described below with reference to the drawings.

FIG. 1 schematically shows the entire structure. In the figure, the DNA sequencer device includes a laser unit 1, a DNA sequencer main unit 2, and a screen input / output device 3. The screen input / output device 3 includes a personal computer 4 and a keyboard 5. These devices are arranged on a table 6.

FIG. 2 shows a schematic configuration of the laser section 1 and the main body section 2. The laser unit 1 is composed of a box 14 having two lasers 11 and 12 for emitting laser light and a rotary mirror 13 for turning the laser light from the lasers 11 and 90 by 90 °. The main body 2 includes a sample storage chamber 15 for storing and moving the sample and a sample chamber 16 for analyzing the sample. These are box-shaped and serve as a DNA sequencer body. In order to seal the sample chamber 16, a sample door 17 is provided so as to be slidable in the vertical direction. A waste liquid door 18 is provided in the sample storage chamber 15.

FIG. 3 shows a sample door 1 of the DNA sequencer main body.
FIG. 4 shows an arrangement when the sample door 17 is closed, and FIG. 4 shows an arrangement when the sample door 17 is closed. In FIG. 3, the hot plate 94, the sample tray holder 34, the drain bottle 69 for storing the waste liquid of the sheath liquid, and the safety lock release button 19 disposed in the sample chamber 16 are visible. Details of these configurations and arrangements will be described later.

FIG. 5 is an explanatory view of the principle showing the overall configuration.

A buffer (electrolyte) is placed on the table 30.
Buffer tank 3 as an electrophoresis buffer tank containing
2 are arranged. In the buffer liquid tank 32,
A platinum electrode 33 is stretched, and the platinum electrode 33 is in contact with the buffer. On the table 10, three sample trays 100A, 100B, 100C are arranged via a sample tray holder 34. As described later with reference to FIG.
It has 48 sample containers. Sample tray 100
A is fixed to the sample tray holder 34 by set screws S1 and S2, and is detachable from the sample tray holder 34. The bottom of the sample tray 100A is made of a conductive metal such as SUS.
It is made of a conductive metal such as S and is electrically connected to the sample tray holder 34. Sample tray 100
Similarly, B and 100C also have 48 sample containers, and the bottoms of the sample trays 100B and 100C are electrically connected to the sample tray holder 34.

Forty-eight capillaries 40 are arranged in parallel, and the inside thereof is filled with a gel matrix for separation. The lower end of the capillary 40 is fixed by a capillary holder 42, and the lower end is inserted into a buffer in the buffer liquid tank 33. The upper end of the capillary 40 is fixed by a capillary holder 44, and the upper end is connected and fixed to a coupler 46.

The coupler 46 is connected to a ground-side electrode plug 52, and the electrode plug 52 is connected to the ground pole of the high-voltage power supply 50. Also, the sample tray holder 34
Is connected to a high-voltage side electrode plug 54, the platinum electrode 33 of the buffer liquid tank 32 is connected to a high-voltage side electrode plug 56, and the electrode plugs 54 and 56 are connected to the high-voltage (-) electrode of the high-voltage power supply 50. It is connected.

The coupler 46 is connected to the sheath flow cell 60. A sheath liquid 64 held in a sheath liquid tank 62 is introduced into the sheath flow cell 60 by gravity. Electrophoretically separated in the capillary 40,
The separated sample flowing out of the electrophoresis end of the capillary 40 is carried to the upper side while the sample components of each capillary are separated from each other by the sheath liquid.

The lateral direction of the sheath flow cell 60 (Y in the figure)
Direction), the laser light emitted from the lasers 11 and 12 is collimated into a parallel light beam by the lens 72 and then irradiated to excite the separated sample in the sheath flow cell 60. A shutter 74 is provided between the lasers 11 and 12 and the lens 72 so that the sample can be selectively excited. The fluorescence generated by the laser beam irradiation is extracted from the X-axis direction orthogonal to the Y-axis direction, collected by the condenser lens 82, and
The light having the wavelength to be detected is selected, and the image is formed on the optical sensor 88 such as a two-dimensional CCD sensor by the imaging lens 86. The signal detected by the optical sensor 88 is sent to the screen processing device 3, which is a signal processing device, where the signal is processed, the type of the terminal base is identified based on the wavelength of the fluorescence, and the base sequence of the nucleic acid sample is determined based on the measurement signal. Is analyzed.

In determining the base sequence of DNA (deoxyribonucleic acid), generally four wavelengths are measured. A fluorescent dye is previously bound by a reaction operation such that each maximum wavelength corresponds to the type of the base at the end of the DNA fragment.

At the upper end of the sheath flow cell 60, a drain adapter 66 is attached, and the sample flowing into the sheath flow cell 60 from the capillary 40 is discharged as waste liquid through the drain tube 67 to the drain bottle 69. . A flow controller 68 including an orifice and a plurality of capillaries is provided in the middle of the drain tube 67, and controls the flow rate while keeping the flow resistance of the drain tube 67 constant.

Next, the overall operation of the electrophoresis analyzer according to the present embodiment will be described.

The sample trays 100A, 100B,
A predetermined amount of an analysis sample is dispensed into each of 48 sample containers of 100C. The sample trays 100A, 100B, and 100C containing the sample are fixed to the sample tray holder 34 by set screws S1 and S2.

With the lower end of the capillary 40 inserted into the sample, the sample tray 100A and the coupler 46
By applying a high voltage from the high voltage power supply 50 during the period, the sample in the sample container is introduced into the capillary 40.

By applying a high voltage from a high voltage power supply 50 between the platinum electrode 33 and the coupler 46 with the lower end of the capillary 40 inserted in the buffer, the sample introduced into the capillary 40 is Separated by electrophoresis.

When the analysis of the samples in the sample tray 100A is completed, the samples in the sample trays 100B and 100C are introduced into the capillaries 40 and separated by electrophoresis in the same procedure as described above.

Since it takes about 2 hours to analyze one sample, as shown in FIG.
4, three sample trays 100A, 100B, 10
By installing 0C, automatic analysis for about 6 hours becomes possible. The number of sample trays is not limited to three, and may be larger.

The method of optically detecting a sample separated by electrophoresis is not limited to fluorescence detection, but may be absorption detection or the like.

Next, the structure of the sample container used in this embodiment will be described with reference to FIG.

FIG. 6 is a plan view showing the arrangement of sample containers used in the electrophoresis analyzer according to one embodiment of the present invention.

The sample tray 100 has a rectangular planar shape and has 48 circular sample containers 100-1 and 100-.
2, ..., 100-48 are linearly arranged. The sample tray 100 includes an upper sample plate 102.
And a metal base 104 fixed below the sample plate 102. The sample plate 102 is formed of a transparent acrylic resin,
Forty-eight circular openings for forming 0-1, 100-2,..., 100-48 are formed. As a material of the sample plate 102, a transparent plastic such as vinyl chloride or polycarbonate can be used. By forming the sample plate 102 from a transparent material, the sample containers 100-1, 100-2,.
The state of the sample accommodated in the sample 100-48 and the state of the capillary inserted into the sample can be easily confirmed visually.

The sample plate 102 is fixed to the metal base 104 with eight screws. Metal base 1
As a material of 04, SUS which is a conductive material is used. The metal base 104 includes the sample containers 100-1, 10
, 100-48, and form part of the wall surfaces of the sample containers 100-1, 100-2,.
8 is used as a common electrode for the sample contained in the sample. By making a part of the sample container a metal base,
It is no longer necessary to insert an electrode into the sample as in the prior art. It has been experimentally confirmed that even if SUS, which is a general-purpose material, is used as the material of the metal base 104, it has sufficient durability. SUS as metal base 104
, The sample tray 100 could be continuously used for more than 6 months only by periodically washing it with water. In the conventional electrophoresis analyzer, the electrode material is noble metal such as platinum, so that the device is expensive. However, by using SUS as the electrode, the sample container can be configured at low cost. .

A circular hole 1 is provided at each end of the metal base 104.
04A is formed, and as described in FIG. 1, the sample tray 100 is used by being fixed to a metal sample tray holder using set screws.

The method for fixing the sample tray 100 to the sample tray holder is not limited to a set screw, but a leaf spring or the like may be used.

In place of the metal base 104, a metal foil formed on an insulating substrate or a metal film formed by a method such as vapor deposition or sputtering can be used in the same manner as the metal base. . At this time, the metal foil or metal film forms a part of the wall surface of the sample container, and establishes electrical continuity with the sample accommodated in the sample container,
Can be used as an electrode.

Sample plate 102 and metal base 10
As described later, a packing is inserted between the sample containers 4 to prevent the sample in the sample container from leaking.

The length L1 of the sample plate 102 is 1
It is 60 mm. A sample container 100-48 is formed from the center of the sample container 100-1 formed on the sample plate 102.
The distance L2 to the center is 141 mm. That is, the pitch P between adjacent sample containers is set to 3 mm.

The pitch P between adjacent sample containers is set to an integral fraction of the pitch (9 mm) of the microtiter plate. That is, generally, a microtiter plate is often used for preparing a sample. The pitch of the pipette for pipetting the sample from the microtiter plate is equal to the pitch of the microtiter plate. When pipetting a sample from a microtiter plate to a sample container using this pipette, the pitch P between the sample containers is set to an integral fraction of the pitch (9 mm) of the microtiter plate, for example, as shown in FIG. , An integer / 1, that is, 1/3, an existing pipette can be used. That is, in the first pipetting, the sample containers 100-1, 100-4,.
Samples are injected into every third sample container, such as 0-46, which is a denominator of integer minutes / 1. In the second pipetting, the pipette was shifted by 3 mm and the sample container 1
The sample is injected into 00-2, 100-5, ..., 100-47. In addition, for the third time, further pipette 3 mm
Shift the sample containers 100-3, 100-6, ..., 1
The sample is injected at 00-48. In this way, although it is necessary to divide the sample into three pipettings, the sample can be injected into the sample container using an existing pipette.

The pitch P between the sample containers is set to an integer fraction of the pitch (9 mm) of the microtiter plate.
One part is appropriate.

FIG. 7 shows the configuration of the main part of the electrophoresis analyzer and the state of its assembly together with the state in which the hot plate is opened. FIG. 8 shows the situation when the hot plate is closed. In these figures, the pedestal 90 includes a vertical plate portion 91 and a flat plate portion 92. A laser beam irradiation member 93 and a hot plate 94 are fixed on the vertical plate portion 91 of the pedestal 90. The sheath flow cell side tip of the capillary 40 is cut, and the cell side tip of the capillary is set in the sheath flow cell 60. Thus, a combination of the capillary 40 and the sheath flow cell 60 is formed. As shown in the drawing, the capillary 40 has a configuration in which the cell-side tip is narrowed and densely packed, and a capillary holder 95 holds the longitudinal direction constant. The sheath flow cell 60 is fixed to the sheath flow cell base 96, and the vertical plate portion 9 of the flow cell base 96 pedestal.
The flow cell 60 and the capillary 40 can be set or placed on the pedestal 90 by being fixed to the base 1 by the stopper 97. The hot plate 94 heats the capillary 40 and controls the temperature.

The sheath flow cell 60, the capillary 40 combined with the sheath flow cell 60, and the hot plate 94 for controlling the temperature of the capillary.
And a group of devices including a laser irradiation port member 93 for irradiating the sheath flow cell 60 with laser light are arranged on one vertical surface. A pedestal 90, that is, a vertical plate portion 91 of the pedestal 90 is used to facilitate the disposition.

The hot plate 94 has a hinge structure 98 and can be opened from one side. Hot plate 9
4 can cover almost the entire surface of the capillary 40.

The earth 20 is placed on the sheath flow cell table 96.
0, tube 202 to which tube 201 from the sheath liquid tube is connected,
203 is provided. A tube 204 connected to the sheath flow cell 60 is connected to a tube 67 connected to the waste liquid bottle 69.
Is provided.

The table 10 has a base 210 made of electrodes thereon, and the buffer liquid tank 3 is placed on the base 210.
3 and the tray holder 34 are placed without being fixed. Then, the sample tray 100 is placed on the tray holder 34 and fixed. The fixing method has been described above. A group of apparatuses including the table 10 thus configured is set, that is, placed on the flat plate portion 92 of the pedestal 90 by sliding the table 10.

The sample containers 100-1,...
A plurality of sample trays 100 each having -48 are arranged on a plane. In order to facilitate its installation, the pedestal 90
That is, the flat plate portion 91 of the pedestal 90 is used, and the table 10 is slidably mounted on the flat plate portion 91.

As described above, the two groups of devices described above
(4) Each of the rectangular plate portions 91 and the flat plate portion 92, which are rectangular in arrangement, is housed in the vertical plate portion 91 and the flat plate portion 92, thereby being compact. Pedestal 90
If a part of is described in more detail, as shown in the figure, the vertical plate portion 91 and the flat plate portion 92 have an integrated configuration,
A U-shaped accommodation room 201 is formed at the connection portion.
The accommodation room 201 accommodates a part of the table 10 so that the capillary 40 and the buffer solution tank 3 can be accommodated.
3 can be arranged on one vertical surface. When the sample tray side of the capillary 40 is cut, the tip is immersed in the buffer solution tank 33, and the seal flow cell table 96 is fixed to the vertical plate portion 91 as described above.

FIG. 9 is an exploded view of the device unit. In the figure, a combination of a pedestal 90 to which a laser irradiation port member 93 and a hot plate 94 are fixed, a cell base 96 having a sheath flow cell 60 and a capillary 40 are fixed without being fixed to the pedestal 90, and quick replacement is possible. It is possible. On the flat side, a table 10
The tray holder 34 to which the buffer liquid tank 32 and the sample tray 100 are fixed is placed on the tray. The table 10 is slidably mounted on the flat plate portion 92, and the buffer liquid tank 32 and the tray holder 34 are fixed on the table 10 without being fixed thereto, so that quick replacement is possible.

That is, the main part of the apparatus having the functions shown in FIG. 5 is compactly constructed by the unitization shown in FIG. 9, and the sheath flow cell 60 (sheath flow cell table 96) and the large use frequency / replacement frequency are required. In this embodiment, the combination of the capillaries 40 is fixedly mounted without being fixed to the vertical plate portion 91, and the tray holder 34 and the buffer liquid tank 32 are fixedly mounted without being fixed on the table 10. There is one feature.

Next, the setting of the sample number of the sample will be described.

The pitch of the holes in the sample tray is 1/3 of the pitch of the eight pipettes. Therefore, 24 samples can be injected in three injections with an eight pipette.

96 holes for thermal cycler (8 × 12)
When injecting a sample from the tray to the sample tray with an 8-micropipette, the sample number is 1) in the following section.
The sample numbers are made to correspond to each other, such as -1, 1) -2, 2) -1, 2) -2, and 3) -1, 3) -2 when samples are injected one by one.

When the “user designated number” is used, the respective sample numbers in FIG. 10 and in Table 1 are ((the following number) + (“sample number of lane # 1”) − 1).

[0059]

[Table 1]

1) -1 When Injecting with 8-Pipette (1)-"Numbering" is changed to "(A1, B1, C1,..., F1, G
1, H1), "(A7, B7, C7, ..., F7, G7,
H7), the “sample sequence” is changed to “A1: 1, B1:
2, C1: 3,... ", 96 samples are measured twice.

The 96-hole tray is placed so that A1 is at the lower left.

[0062]

[Table 2]

When injecting the samples in the columns of Table 2,
The eight micropipette is set in the hole indicated by ● in FIG. When injecting samples from other rows, align the left end of the pipette with the corresponding numbered hole in FIG.

The file name is added with the following number as shown in Table 3 after the character string of the sample name [fixed part].

[0065]

[Table 3]

1) -2 When Injecting with 8-Pipette (2)-"Numbering" is changed to "(A1, B1, C1,..., F1, G
1, H1), "(A7, B7, C7, ..., F7, G7,
H7), the “sample sequence” is changed to “A1: 1, A2:
2, A3: 3,... ”, The injection into the sample tray is performed in the same manner as in 1) -1.

The file name is added with the following number as shown in Table 4 after the character string of the sample name [fixed part].

[0068]

[Table 4]

2) -1 When Injecting with 8 Pipettes (3)-"Numbering" is changed to "(H1, G1, F1,..., C1, B
1, A1), "(H7, G7, F7, ..., C7, B7,
A7), the “sample sequence” is changed to “A1: 1, B1:
2, C1: 3,... ", 96 samples are measured twice.

The 96-hole tray is placed so that A1 is at the upper right.

[0071]

[Table 5]

In the case of injecting the sample in column ■ of Table 5,
The eight micropipette is set in the hole indicated by ● in FIG. When injecting samples from other rows, align the left end of the pipette with the corresponding numbered hole in FIG.

At this time, the following numbers are added to the file name after the character string of the sample name [fixed part] as shown in Table 6.

[0074]

[Table 6]

2) -2 When Injecting with 8-Pipette (4)-"Numbering" is changed to "(H1, G1, F1,..., C1, B
1, A1), "(H7, G7, F7, ..., C7, B7,
A7), "Sample sequence" is changed to "A1: 1, A2:
2, A3: 3,... ”, The injection into the sample tray is performed in the same manner as in 2) -1.

The file name is added with the following number as shown in Table 7 after the character string of the sample name [fixed part].

[0077]

[Table 7]

3) When injecting one by one (1) 3) -1 When "numbering" is set to "continuous (01 to 48)" (the same number is selected for "sample sequence" regardless of which one is selected). 0) sequentially from the sample data on the left side of the sample tray
1,02, ... 47,48.

3) -2 "Numbering" is changed to "continuous (49-9)
6) ”(the same number is added to either of the“ Sample sequence ”) 4 from the sample data on the left side of the sample tray
9, 50, ... 95, 96.

[Brief description of the drawings]

FIG. 1 shows a schematic diagram of the entire configuration.

FIG. 2 shows a schematic diagram of a partial configuration of FIG.

FIG. 3 shows an arrangement configuration diagram of the main body (door opened).

FIG. 4 shows an arrangement configuration diagram of the main body (door closed).

FIG. 5 shows a principle explanatory diagram.

FIG. 6 is a plan view showing the arrangement of sample containers.

FIG. 7 is a configuration diagram showing a main part configuration (hot plate open) of the electrophoresis analyzer.

FIG. 8 is a configuration diagram showing a main part configuration (hot plate closed) of the electrophoresis analyzer.

FIG. 9 shows an exploded view of the device unit.

FIG. 10 shows a drawing of one of the screen input devices.

FIG. 11 shows a sample number setting diagram of a sample.

FIG. 12 shows a sample number setting diagram of a sample.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Laser part, 2 ... DNA sequencer main part, 3 ... Screen input / output device, 4 ... Personal computer, 5 ... Keyboard, 11, 1
2 laser, 13 mirror, 14 box, 15 sample chamber, 16 sample chamber, 17 sample door, 30 table, 32 buffer solution tank, 34 sample tray holder, 40 capillary , 42,95 ... capillary retainer, 60,96 ... sheath flow cell, 66 ... drain adapter, 67 ... drain tube, 69 ... drain bottle, 90 ... pedestal, 91 ... vertical plate part, 92 ... flat plate part, 93
... Laser beam irradiation member, 94 ... Hot plate, 97 ...
Stoppers, 100: sample tray, 100-1: sample container, 102: sample plate, 200: ground, 20
1 ... sheath liquid tube, 202, 203, 204 ... tube, 210
... A base made of electrodes.

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Isamu Takekoshi 1-280 Higashi Koigakubo, Kokubunji-shi, Tokyo Inside the Design Laboratory, Hitachi, Ltd. Within the Measuring Instruments Division of the Works (72) Inventor Daizo Tokinaga 882 Ma, Hitachinaka-shi, Ibaraki Pref.Hitachi Co., Ltd. (72) Inventor Satoshi Takahashi 882 Ma, Hitachinaka-shi, Ibaraki Pref. Hitachi, Ltd.Measurement Division

Claims (5)

[Claims] An electrophoresis analyzer for optically electrophoretically analyzing a plurality of sample components, comprising: a sheath flow cell table having a pedestal constituted by a vertical plate portion and a flat plate portion; A group consisting of a capillary combined with a sheath flow cell, a hot plate for controlling the temperature of the capillary, and a laser irradiation member for irradiating the sheath flow cell with laser light is mounted on the vertical plate portion of the pedestal, and a buffer liquid tank and A sample tray accommodating a sample was placed on a table, the table was set on a flat plate portion of the pedestal, and the group and the table, which were set as described above, were formed in the main body of the analyzer together with the pedestal. An electrophoresis analyzer which is arranged in a sample chamber. 2. An electrophoresis analyzer for optically electrophoretically analyzing a plurality of sample components, comprising: a sheath flow cell, a capillary combined with the sheath flow cell, a hot plate unit for controlling the temperature of the capillary, and the sheath flow cell. A group consisting of laser irradiation port members for irradiating laser light is arranged on a vertical surface, a plurality of sample trays having sample containers arranged in parallel are arranged on a plane, and the two are arranged. An electrophoresis analyzer, wherein a buffer solution tank is disposed at a line where the surfaces intersect, and a tip of the capillary is immersed in a solution in the buffer solution tank. 3. An electrophoresis analyzer for optically electrophoretically analyzing a plurality of sample components, comprising a pedestal comprising a vertical plate portion and a flat plate portion, wherein a laser beam is applied to the vertical plate portion of the pedestal. A laser irradiation port member for irradiation and a hot plate for controlling the temperature are fixed, and a combined body of a sheath flow cell and a capillary is irradiated with a laser beam to the hot cell, so that the hot cell heats the capillary, A plurality of sample trays are fixed on a tray holder, the tray holder and the buffer liquid tank are mounted on a table without being fixed, and the table is formed on the main body of the analyzer. An electrophoretic analyzer characterized in that it can be slid and mounted without being fixed to a holding section of a sample chamber. 4. An electrophoresis analyzer for optically electrophoretically analyzing a plurality of sample components, a box housing a laser emitting a laser beam, a sample storage chamber for storing a sample, and a sample chamber for analyzing a sample. The sample chamber section includes a sheath flow cell, a capillary combined with the sheath flow cell, and a hot temperature controller for controlling the temperature of the capillary. It is composed of a unit with a plate and a laser irradiation port for irradiating the sheath flow cell with laser light, and is composed of a group arranged in a vertical direction, a buffer solution tank and a sample tray containing a DNA sample, and a horizontal direction. Another group to be provided is provided, and an opening / closing door of the sample chamber portion is provided so as to be slidable in a vertical direction. An electrophoresis analyzer characterized by being carried out. 5. An electrophoretic analysis method for optically electrophoretically analyzing a plurality of sample components, wherein a sample is prepared using a microtiter plate, and a pitch P between adjacent sample containers is set to a pitch of the microtiter plate. Preparing a sample tray that has been reduced to an integral number of 1, the pitch of the pipette for pipetting the sample prepared from the microtiter plate is made equal to the pipette of the microtiter plate, and the sample container is transferred from the microtiter plate to the sample container. When pipetting a sample, the sample is divided and pipetted at a denominator number that is a fraction of an integer, and a sheath flow cell arranged in a vertical direction in parallel with the sample tray and a capillary combined with the sheath flow cell By inhaling the pipetted sample Electrophoretic analysis wherein the performing.