JP4070301B2 - Electrophoresis analyzer and analysis method - Google Patents

Description

【００６０】
１)−１ ８連ピペットで注入する場合（１）
−「番号付け」を「（Ａ１，Ｂ１，Ｃ１，…，Ｆ１，Ｇ１，Ｈ１）」「（Ａ７，Ｂ７，Ｃ７，…，Ｆ７，Ｇ７，Ｈ７）」に、「サンプル配列」を「Ａ１：１，Ｂ１：２，Ｃ１：３，…」に設定したとき９６サンプルを２回に分けて測定する。
【００６１】
Ａ１が左下になるように９６穴トレイを置く。
【００６２】
【表２】

【００６３】
表２の▲１▼の列のサンプルを注入する場合、８連マイクロピペットは図１１の●の穴に合わせる。その他の列のサンプルを注入するときは、図１１の該当する番号の穴にピペットの左端を合わせる。
【００６４】
ファイル名は、サンプル名［固定部］の文字列の後に、次の番号が表３に示されるように付加される。
【００６５】
【表３】

【００６６】
１)−２ ８連ピペットで注入する場合（２）
−「番号付け」を「（Ａ１，Ｂ１，Ｃ１，…，Ｆ１，Ｇ１，Ｈ１）」「（Ａ７，Ｂ７，Ｃ７，…，Ｆ７，Ｇ７，Ｈ７）」に、「サンプル配列」を「Ａ１：１，Ａ２：２，Ａ３：３，…」に設定したときサンプルトレイへの注入は１）−１と同様に行う。
【００６７】
ファイル名は、サンプル名［固定部］の文字列の後に、次の番号が表４に示されるように付加される。
【００６８】
【表４】

【００６９】
２)−１ ８連ピペットで注入する場合（３）
−「番号付け」を「（Ｈ１，Ｇ１，Ｆ１，…，Ｃ１，Ｂ１，Ａ１）」「（Ｈ７，Ｇ７，Ｆ７，…，Ｃ７，Ｂ７，Ａ７）」に、「サンプル配列」を「Ａ１：１，Ｂ１：２，Ｃ１：３，…」に設定する場合９６サンプルを２回に分けて測定する。
【００７０】
Ａ１が右上になるように９６穴トレイを置く。
【００７１】
【表５】

【００７２】
表５の■の列のサンプルを注入する場合、８連マイクロピペットは図１２の●の穴に合わせる。その他の列のサンプルを注入するときは、図１２の該当する番号の穴にピペットの左端を合わせる。
【００７３】
この時、ファイル名は、サンプル名［固定部］の文字列の後に、次の番号が表６に示されるように付加される。
【００７４】
【表６】

【００７５】
２)−２ ８連ピペットで注入する場合（４）
−「番号付け」を「（Ｈ１，Ｇ１，Ｆ１，…，Ｃ１，Ｂ１，Ａ１）」「（Ｈ７，Ｇ７，Ｆ７，…，Ｃ７，Ｂ７，Ａ７）」に、「サンプル配列」を「Ａ１：１，Ａ２：２，Ａ３：３，…」に設定したときサンプルトレイへの注入は２）−１と同様に行う。
【００７６】
ファイル名は、サンプル名［固定部］の文字列の後に、次の番号が表７に示されるように付加される。
【００７７】
【表７】

【００７８】
３) １本ずつ注入するとき（１）
３)−１ 「番号付け」を「連続（０１〜４８）」に設定する場合
（「サンプル配列」はどちらを選択しても同じ番号が付加される）
サンプルトレイの左側のサンプルのデータから順に０１，０２，…４７，４８となる。
【００７９】
３)−２ 「番号付け」を「連続（４９〜９６）」に設定する場合
（「サンプル配列」はどちらを選択しても同じ番号が付加される）
サンプルトレイの左側のサンプルのデータから順に４９，５０，…９５，９６となる。
【図面の簡単な説明】
【図１】全体構成概略図を示す。
【図２】図１の一部構成の概略図を示す。
【図３】本体の配置構成図（扉開放）を示す。
【図４】本体の配置構成図（扉閉鎖）を示す。
【図５】原理説明図を示す。
【図６】試料容器の配置を示す平面図である。
【図７】電気泳動分析装置の主要部構成（ホットプレート開放）を示す構成図である。
【図８】電気泳動分析装置の主要部構成（ホットプレート閉鎖）を示す構成図である。
【図９】装置ユニットの分解図を示す。
【図１０】画面入力装置の一つの図面図を示す。
【図１１】試料のサンプル番号設定図を示す。
【図１２】試料のサンプル番号設定図を示す。
【符号の説明】
１…レーザ部、２…ＤＮＡシーケンサ本体部、３…画面入出力装置、４…パソコン、５…キーボード、１１，１２…レーザ、１３…ミラー、１４…箱体、１５…試料収納室部、１６…試料室部、１７…試料扉、３０…テーブル、３２…バッファ液タンク、３４…サンプルトレイホルダ、４０…キャピラリー、４２，９５…キャピラリー押さえ、６０，９６…シースフローセル、６６…ドレインアダプタ、６７…ドレインチューブ、６９…ドレイン瓶、９０…台座、９１…縦板部、９２…平面板部、９３…レーザ光線照射部材、９４…ホットプレート、９７…止め具、１００…サンプルトレイ、１００−１…試料容器、１０２…サンプルプレート、２００…アース、２０１…シース液管、２０２，２０３，２０４…管、２１０…電極からなる台部。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an electrophoresis analysis apparatus and an analysis method. In particular, the present invention relates to an electrophoresis apparatus and analysis method suitable for use in a DNA sequencer (DNA base sequence analyzer) that analyzes a biological sample such as DNA (deoxyribonucleic acid) using a plurality of capillaries or fine channels as an electrophoresis medium.
[0002]
[Prior art]
2. Description of the Related Art DNA analysis technology based on electrophoresis, in particular, DNA sequencers (DNA base sequence analyzers) have become widespread. As analysis needs increase, the need to improve analysis throughput is increasing. One method for improving the analysis throughput is to integrate the electrophoretic medium.
[0003]
JP-A-6-138037 discloses an electrophoresis apparatus in which a sample separation part provided between a cathode electrode tank and an anode electrode tank to which a voltage is applied by a power source is an electrophoresis path composed of capillaries, each having one end. The cathode electrode tank or the other end of the capillary pair connected to the anode electrode tank is placed in the optical cell so as to face each other while maintaining a certain gap while keeping the axis substantially coincident, and the electrophoresis path passing through the optical cell. A sheath liquid container provided outside the optical cell, and an electrophoresis separation capillary end which is an upstream migration part which is a sample separation part by injecting the sheath liquid into the optical cell from the sheath liquid container A sheath flow forming means for guiding a sample to be migrated from the tube to a facing capillary in a sheath flow state, and detecting the sample by irradiating the optical detection portion with light from a light source using the gap portion as an optical detection portion A plurality of optical detection units having a detection means and formed by a plurality of capillary pairs are arranged in the optical cell, and the plurality of optical detection units are connected to each other via an electrolytic solution; A plurality of capillary pairs arranged in a line so that they are positioned on a straight line, and excitation light is irradiated along the straight line so as to irradiate all optical detectors simultaneously, and the sample flows out of each capillary An electrophoretic device that simultaneously detects the fluorescence emitted from the liquid is described.
[0004]
As a second method, a multi-channel method is proposed in which fine grooves are formed on the surface of a glass plate and used as a migration path, as described in US Pat. No. 5,924,212 and JP-A-5-93711. Has been. Even in this method, a large number of samples can be separated and analyzed simultaneously and at high speed.
[0005]
In the multichannel method as described above, first, an electrode and one end of a capillary are inserted into a sample contained in a sample container, and then a voltage is applied to both ends of the capillary to electrically move the sample. The sample is introduced into the capillary, and then the electrode and one end of the capillary are inserted into the buffer solution in the buffer tank, and then a voltage is applied to both ends of the capillary to separate the sample by electrophoresis.
[0006]
[Problems to be solved by the invention]
If the number of samples to be analyzed simultaneously is several tens in order to improve the analysis throughput, the work of inserting electrodes and capillaries becomes difficult.
[0007]
It is an object of the present invention to provide an electrophoretic analyzer which can be easily handled by using a multi-capillary method so that the structure and configuration of the apparatus are compact and easy to handle, and an analysis method which is easy to use. And
[0008]
[Means for Solving the Problems]
In the present invention, a capillary and a sheath flow cell combined therewith are arranged in a vertical direction, a sample plate on which a sample is placed is arranged in a planar direction, a buffer liquid tank is arranged on a line where both arrays intersect, and the inside of the buffer liquid tank It is characterized in that both are joined via a liquid. Each device group constituting the two arrays was placed on a pedestal composed of a vertical plate portion and a flat plate portion, so that the device could be easily assembled and used. In addition, the present invention distinguishes the method of mounting on a pedestal between a frequently used device and a less frequently used device, and a frequently used device such as a combination of a capillary and a sheath flow cell or a sample plate on the pedestal. It was supposed to be placed without sticking.
[0009]
Further, the present invention can adopt an improved pipetting method without changing an existing pipette by adopting such an apparatus structure.
[0010]
Specifically, the present invention provides the following apparatuses and methods.
[0011]
The present invention relates to an electrophoretic analyzer for optically analyzing a plurality of sample components, a sheath flow cell base having a pedestal composed of a vertical plate portion and a flat plate portion, and having a sheath flow cell, and the sheath A group consisting of a capillary combined with a flow cell, a hot plate for controlling the temperature of the capillary, and a laser irradiation port member for irradiating the sheath flow cell with a laser beam is mounted on the vertical plate portion of the pedestal, and a buffer liquid tank and a sample A sample tray for storing the sample on the table, the table mounted on the flat plate portion of the pedestal, and the group and the table mounted as described above formed on the analyzer main body together with the pedestal An electrophoretic analyzer disposed in a chamber is provided.
[0012]
The present invention provides an electrophoretic analyzer for optically analyzing a plurality of sample components in a sheath flow cell, a capillary combined with the sheath flow cell, a hot plate unit for controlling the temperature of the capillary, and a laser in the sheath flow cell. A group of laser irradiation port members for irradiating light is disposed on a vertical surface, a plurality of sample trays having parallel sample containers are disposed on a plane, and two surfaces on which both are disposed An electrophoretic analyzer is provided in which a buffer liquid tank is disposed at a line where the two intersect, and the tip of the capillary is immersed in the liquid in the buffer liquid tank.
[0013]
The present invention relates to an electrophoretic analyzer for optically analyzing a plurality of sample components, having a pedestal composed of a vertical plate portion and a flat plate portion, and irradiating the vertical plate portion of the pedestal with laser light. A laser irradiation port member that performs temperature control and a hot plate that controls temperature are fixed, and a combination of a sheath flow cell and a capillary is applied to the vertical plate so that a laser beam irradiates the sheath flow cell and the hot plate heats the capillary. Place the sample tray on the tray holder, place the tray holder and the buffer solution tank on the table without sticking, and form the table on the analyzer body Provided is an electrophoretic analyzer which can be slid and placed without being fixed to a holding part of a sample chamber.
[0014]
The present invention relates to an electrophoretic analyzer for optically analyzing a plurality of sample components, a box housing a laser emitting laser light, a sample storage chamber for storing a sample, and a sample chamber for analyzing a sample. The sample chamber unit includes a sheath flow cell, a capillary combined with the sheath flow cell, and a hot plate for controlling the temperature of the capillary. And a laser irradiation port for irradiating the sheath flow cell with laser light, and a group arranged in the vertical direction, a buffer liquid tank and a sample tray for storing the DNA sample, and arranged in the horizontal direction. And a group of other doors provided, and the open / close door of the sample chamber is slidable in the vertical direction. Providing an electrophoretic analyzer that.
[0015]
The present invention provides an electrophoretic analysis method for optically analyzing a plurality of sample components by preparing a sample using a microtiter plate, and setting a pitch P between adjacent sample containers to a pitch of the microtiter plate. Prepare a sample tray with a fraction of an integer, and make the pitch of the pipette for pipetting the prepared sample from the microtiter plate equal to the pipette of the microtiter plate, and the sample from the microtiter plate to the sample container When pipetting the sample, the sample is divided and pipetted with the number of denominators of a fraction of an integer, and the sheath flow cell arranged in the vertical direction in parallel with the sample tray and the capillary combined with the sheath flow cell Analysis by inhaling the pipetted sample To provide a cormorant electrophoretic analysis method.
[0016]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, an embodiment of the present invention will be described with reference to the drawings.
[0017]
FIG. 1 shows an overall configuration outline. In the figure, the DNA sequencer device is composed of a laser unit 1, a DNA sequencer main body unit 2, and a screen input / output device 3. The screen input / output device 3 comprises a personal computer 4 and a keyboard 5. These devices are arranged on a table 6.
[0018]
FIG. 2 shows a schematic configuration of the laser unit 1 and the main body unit 2. The laser unit 1 includes a box 14 having two lasers 11 and 12 that emit laser light and a rotary mirror 13 that changes the direction of the laser light from the two lasers 11 and 12. The main body 2 includes a sample storage chamber 15 for storing and moving the sample, and a sample chamber 16 for analyzing the sample. These form a box shape and become a DNA sequencer body. In order to seal the sample chamber 16, a sample door 17 is provided so as to be slidable in the vertical direction. The sample storage chamber 15 is provided with a waste liquid door 18.
[0019]
FIG. 3 shows an arrangement configuration when the sample door 17 of the DNA sequencer main body is opened, and FIG. 4 shows an arrangement configuration when the sample door 17 is closed. In FIG. 3, a hot plate 94, a sample tray holder 34, a drain bottle 69 as a sheath liquid waste reservoir, and a safety lock release button 19 can be seen. Details of these configurations and arrangements will be described later.
[0020]
FIG. 5 is an explanatory diagram of the principle showing the overall configuration.
[0021]
On the table 30, a buffer liquid tank 32 is arranged as an electrophoresis buffer tank containing a buffer (electrolytic solution). A platinum electrode 33 is stretched in the buffer liquid tank 32, and the platinum electrode 33 is in contact with the buffer. Further, three sample trays 100A, 100B, and 100C are arranged on the table 10 via the sample tray holder 34. As will be described later with reference to FIG. 2, the sample tray 100A includes 48 sample containers. The sample tray 100A is fixed to the sample tray holder 34 by set screws S1 and S2, and can be detached from the sample tray holder 34. The bottom of the sample tray 100A is made of a conductive metal such as SUS, and is made of a conductive metal such as SUS and is electrically connected to the sample tray holder 34. Similarly, the sample trays 100B and 100C are provided with 48 sample containers, and the bottoms of the sample trays 100B and 100C are electrically connected to the sample tray holder 34.
[0022]
Forty-eight capillaries 40 are arranged in parallel, and the inside thereof is filled with a gel matrix for separation. The lower end side of the capillary 40 is fixed by a capillary presser 42, and the lower end thereof is inserted into a buffer in the buffer liquid tank 33. The upper end side of the capillary 40 is fixed by a capillary presser 44, and the upper end thereof is connected and fixed to a coupler 46.
[0023]
The coupler 46 is connected to the electrode plug 52 on the ground side, and the electrode plug 52 is connected to the ground electrode of the high voltage power supply 50. The sample tray holder 34 is connected to the high-voltage side electrode plug 54, the platinum electrode 33 of the buffer liquid tank 32 is connected to the high-voltage side electrode plug 56, and the electrode plugs 54, 56 are connected to the high-voltage power supply 50. Connected to the high voltage (-) pole.
[0024]
The coupler 46 is connected to the sheath flow cell 60. The sheath fluid 64 held in the sheath fluid tank 62 is introduced into the sheath flow cell 60 by gravity. The separated sample that is separated by electrophoresis in the capillary 40 and flows out from the migration terminal portion of the capillary 40 is carried to the upper side while the sample components of each capillary are separated from each other by the sheath liquid.
[0025]
From the side surface direction (Y direction in the figure) of the sheath flow cell 60, the laser light emitted from the lasers 11 and 12 is collimated into a parallel light beam by the lens 72, irradiated, and separated in the sheath flow cell 60. Excited. A shutter 74 is provided between the lasers 11 and 12 and the lens 72 so that the sample can be selectively excited. The fluorescence generated by the laser light irradiation is extracted from the X-axis direction orthogonal to the Y-axis direction, collected by the condensing lens 82, light having a wavelength to be detected by the filter 84, and the imaging lens 86. Thus, an image is formed on an optical sensor 88 such as a two-dimensional CCD sensor. The signal detected by the optical sensor 88 is sent to the screen processing device 3 which is a signal processing device, where the signal is processed, the type of terminal base is identified by the wavelength of the fluorescence, and the base sequence of the nucleic acid sample is based on the measurement signal Is analyzed.
[0026]
In determining the base sequence of DNA (deoxyribonucleic acid), generally, four wavelengths are measured. A fluorescent dye is bound in advance by a reaction operation so that each maximum wavelength corresponds to the type of base at the end of the DNA fragment.
[0027]
A drain adapter 66 is attached to the upper end of the sheath flow cell 60, and the sample flowing into the sheath flow cell 60 from the capillary 40 is discharged as a waste liquid to the drain bottle 69 through the drain tube 67. A flow controller 68 including an orifice and a plurality of capillaries is provided in the middle of the drain tube 67, and the flow rate is controlled with the flow path resistance of the drain tube 67 being constant.
[0028]
Next, the overall operation of the electrophoretic analyzer according to the present embodiment will be described.
[0029]
A predetermined amount of analysis sample is dispensed in advance into 48 sample containers of each of the sample trays 100A, 100B, and 100C. The sample trays 100A, 100B, and 100C in which the samples are stored are fixed to the sample tray holder 34 with set screws S1 and S2.
[0030]
The sample in the sample container is introduced into the capillary 40 by applying a high voltage from the high voltage power supply 50 between the sample tray 100A and the coupler 46 with the lower end of the capillary 40 inserted into the sample. The
[0031]
When a high voltage is applied from the high voltage power source 50 between the platinum electrode 33 and the coupler 46 with the lower end of the capillary 40 inserted in the buffer, the sample introduced into the capillary 40 is electrophoresed. To be separated.
[0032]
When the analysis of the sample in the sample tray 100A is completed, the sample in the sample trays 100B and 100C is introduced into the capillary 40 and separated by electrophoresis in the same procedure as described above.
[0033]
Since it takes about 2 hours to analyze one sample, as shown in FIG. 5, by installing three sample trays 100A, 100B, and 100C on the sample tray holder 34, about 6 hours can be obtained. Automatic analysis is possible. The number of sample trays is not limited to three and may be larger.
[0034]
Further, the optical detection method of the electrophoretically separated sample is not limited to fluorescence detection, and absorbance detection can also be used.
[0035]
Next, the configuration of the sample container used in the present embodiment will be described with reference to FIG.
[0036]
FIG. 6 is a plan view showing the arrangement of sample containers used in the electrophoretic analyzer according to one embodiment of the present invention.
[0037]
The sample tray 100 has a rectangular planar shape, and 48 circular sample containers 100-1, 100-2, ..., 100-48 are linearly arranged. The sample tray 100 includes an upper sample plate 102 and a metal base 104 fixed below the sample plate 102. The sample plate 102 is formed of a transparent acrylic resin, and 48 circular openings for forming the sample containers 100-1, 100-2,..., 100-48 are formed. As a material of the sample plate 102, transparent plastic such as vinyl chloride or polycarbonate can be used. By forming the sample plate 102 from a transparent material, it is easy to visually check the state of the sample accommodated in the sample containers 100-1, 100-2, ..., 100-48 and the capillaries inserted into the sample. Can be confirmed.
[0038]
The sample plate 102 is fixed to the metal base 104 with eight screws. As the material of the metal base 104, SUS which is a conductive material is used. The metal base 104 forms part of the wall surface of the sample containers 100-1, 100-2,..., 100-48, and is accommodated in the sample containers 100-1, 100-2,. It is used as a common electrode for the sample. By using a part of the sample container as a metal base, there is no need to insert an electrode into the sample as in the prior art. It has been experimentally confirmed that even when SUS, which is a versatile material, is used as the material of the metal base 104, it has sufficient durability. By using SUS as the metal base 104 and periodically washing the sample tray 100 with water, continuous use for 6 months or more was possible. In the conventional electrophoretic analyzer, a noble metal such as platinum is used as an electrode material, so the apparatus is expensive. However, by using SUS as an electrode, a sample container can be configured at low cost. .
[0039]
Circular holes 104A are formed at both ends of the metal base 104. As described in FIG. 1, the sample tray 100 is used by being fixed to a metallic sample tray holder using a set screw. .
[0040]
The method of fixing the sample tray 100 to the sample tray holder is not limited to a set screw, and a plate spring or the like can be used.
[0041]
In place of the metal base 104, a metal foil may be formed on an insulating substrate, or a metal film may be formed by a method such as vapor deposition or sputtering. At this time, the metal foil or the metal film can be used as an electrode by forming a part of the wall surface of the sample container and taking electrical continuity with the sample accommodated in the sample container.
[0042]
As will be described later, a packing is inserted between the sample plate 102 and the metal base 104 to prevent the sample in the sample container from leaking.
[0043]
The length L1 of the sample plate 102 is 160 mm. A distance L2 from the center of the sample container 100-1 formed on the sample plate 102 to the center of the sample container 100-48 is 141 mm. That is, the pitch P between adjacent sample containers is 3 mm.
[0044]
The pitch P between adjacent sample containers is set to 1 / integer of the pitch (9 mm) of the microtiter plate. That is, in general, a microtiter plate is often used for sample preparation. The pitch of the pipette that pipettes the sample from the microtiter plate is equal to the pitch of the microtiter plate. When pipetting a sample from a microtiter plate to a sample container using this pipette, the pitch P between the sample containers is set to 1 / integer of the pitch (9 mm) of the microtiter plate, for example, as shown in FIG. An existing pipette can be used by setting an integral part / 1 or 1/3. That is, in the first pipetting, the sample is injected into every third sample container that is an integer / 1 denominator, such as sample containers 100-1, 100-4,..., 100-46. In the second pipetting, the pipette is shifted by 3 mm, and the sample is injected into the sample containers 100-2, 100-5, ..., 100-47. Further, in the third time, the pipette is further shifted by 3 mm, and the sample is injected into the sample containers 100-3, 100-6, ..., 100-48. In this way, although it is necessary to separate the pipetting of the sample into three times, the sample can be injected into the sample container using an existing pipette.
[0045]
The pitch P between the sample containers is set to 1 / integer of the pitch (9 mm) of the microtiter plate. In consideration of the amount and size of the sample used, in most cases, 1/2 to 1/4 is appropriate.
[0046]
FIG. 7 shows the configuration of the main part of the electrophoretic analyzer and the state of assembly thereof together with the state where the hot plate is opened. FIG. 8 shows the situation when the hot plate is closed. In these drawings, the pedestal 90 includes a vertical plate portion 91 and a flat plate portion 92. A laser beam irradiation port member 93 and a hot plate 94 are fixed on the vertical plate portion 91 of the base 90. The tip of the capillary 40 on the sheath flow cell side is cut, and the tip of the capillary on the cell side is set in the sheath flow cell 60. Thus, a combination of the capillary 40 and the sheath flow cell 60 is configured. As shown in the drawing, the capillary 40 has a structure in which the cell-side tip portion is narrowed to be closely packed, and the longitudinal direction is held constant by the capillary presser 95. The sheath flow cell 60 is fixed to the sheath flow cell base 96, and the flow cell 60 and the capillary 40 can be set on the base 90 by being fixed to the vertical plate portion 91 of the flow cell base 96 by a stopper 97. The hot plate 94 heats the capillary 40 and controls its temperature.
[0047]
A group of devices including a sheath flow cell 60, a capillary 40 combined with the sheath flow cell 60, a unit of a hot plate 94 for controlling the temperature of the capillary, and a laser irradiation port member 93 for irradiating the sheath flow cell 60 with laser light are one group. Arranged on two vertical surfaces. In order to facilitate the arrangement, a pedestal 90, that is, a vertical plate portion 91 of the pedestal 90 is used.
[0048]
The hot plate 94 has a hinge configuration 98 and can be opened from one side. The hot plate 94 can cover almost the entire surface of the capillary 40.
[0049]
On the sheath flow cell base 96, pipes 202 and 203 to which a ground 200 and a pipe 201 from a sheath liquid pipe are connected are provided. The sheath flow cell 60 is provided with a pipe 204 to which a pipe 67 connected to the waste liquid bottle 69 is connected.
[0050]
The table 10 includes a base portion 210 made of an electrode thereon, and the buffer liquid tank 33 and the tray holder 34 are placed on the base portion 210 without being fixed. Then, the sample tray 100 is placed on and fixed to the tray holder 34. The fixing method has been described above. The group of apparatuses including the table 10 configured as described above is set, that is, placed on the flat plate portion 92 of the base 90 by sliding the table 10.
[0051]
A plurality of sample trays 100 including sample containers 100-1,..., 100-48 arranged in parallel are arranged on a plane. In order to facilitate the arrangement, the base 90, that is, the flat plate portion 91 of the base 90 is used, and the table 10 is slidably mounted on the flat plate portion 91.
[0052]
As described above, the above-described two groups of devices are accommodated in the vertical plate portion 91 and the flat plate portion 92 that are each 90 ° and rectangular, and are made compact. A part of the pedestal 90 will be described in more detail. As shown in the figure, the vertical plate portion 91 and the flat plate portion 92 are integrated, and a U-shaped accommodation chamber 201 is formed at the connecting portion. By accommodating a part of the table 10 in the accommodation chamber 201, the connection between the capillary 40 and the buffer liquid tank 33 can be arranged on one vertical surface. The sample tray side of the capillary 40 is cut and its tip is immersed in the buffer liquid tank 33, and the seal flow cell base 96 is fixed to the vertical plate portion 91 as described above.
[0053]
FIG. 9 is an exploded view of the device unit. In the figure, the apparatus unit is fixed without the base 90 to which the laser irradiation port member 93 and the hot plate 94 are fixed, the combination of the cell base 96 having the sheath flow cell 60 and the capillary 40 being fixed to the base 90, and can be quickly exchanged. It is possible. On the flat surface side, a tray holder 34 having a buffer liquid tank 32 and a sample tray 100 fixed thereon is placed on the table 10. The table 10 is slidably mounted on the flat plate portion 92, and the buffer liquid tank 32 and the tray holder 34 are fixed on the table 10 without being fixed and can be exchanged quickly.
[0054]
That is, the main part of the apparatus having the function shown in FIG. 5 is compactly configured by the unitization shown in FIG. 9, and the sheath flow cell 60 (sheath flow cell base 96) and the capillary 40 that require a large use frequency and exchange frequency are required. One feature of the present embodiment is that the combined body is fixedly mounted without being fixed to the vertical plate portion 91, and the tray holder 34 and the buffer liquid tank 32 are fixedly mounted without being fixed on the table 10. There is.
[0055]
Next, setting of the sample number of the sample will be described.
[0056]
The pitch of the holes in the sample tray is 1/3 of the pitch of the 8 pipettes. Therefore, it is possible to inject 24 samples in 3 injections with an 8-unit pipette.
[0057]
When injecting a sample from the 96-hole (8x12) tray for the thermal cycler into the sample tray with an 8-unit micropipette, the sample numbers are 1) -1, 1) -2, 2) -1, 2) in the following sections. -2 and when injecting samples one by one, the sample numbers are made to correspond to 3) -1, 3) -2.
[0058]
When “user-specified number” is used, the sample numbers in FIG. 10 and Table 1 are ((number below) + (“sample number of lane # 1”) − 1) −1.
[0059]
[Table 1]

[0060]
1) -1 When injecting with an 8 pipette (1)
-“Numbering” is “(A1, B1, C1,..., F1, G1, H1)” “(A7, B7, C7,..., F7, G7, H7)” and “Sample arrangement” is “A1: 96 samples are divided into two measurements when set to “1, B1: 2, C1: 3,.
[0061]
Place the 96-hole tray so that A1 is on the lower left.
[0062]
[Table 2]

[0063]
When injecting the sample in row (1) of Table 2, the 8-spindle micropipette is aligned with the hole marked with ● in FIG. When injecting samples in other rows, align the left end of the pipette with the corresponding numbered hole in FIG.
[0064]
The file name is appended as shown in Table 3 after the character string of the sample name [fixed part].
[0065]
[Table 3]

[0066]
1) -2 When injecting with an 8 pipette (2)
-“Numbering” is “(A1, B1, C1,..., F1, G1, H1)” “(A7, B7, C7,..., F7, G7, H7)” and “Sample arrangement” is “A1: When “1, A2: 2, A3: 3,...” Is set, injection into the sample tray is performed in the same manner as 1) -1.
[0067]
The file name is appended as shown in Table 4 after the character string of the sample name [fixed part].
[0068]
[Table 4]

[0069]
2) -1 When injecting with an 8 pipette (3)
“Numbering” is “(H1, G1, F1,..., C1, B1, A1)” “(H7, G7, F7,..., C7, B7, A7)”, and “Sample arrangement” is “A1: In the case of setting “1, B1: 2, C1: 3,...”, 96 samples are measured in two portions.
[0070]
Place the 96-hole tray so that A1 is on the upper right.
[0071]
[Table 5]

[0072]
When injecting the sample in the row of ■ in Table 5, the 8-spinned micropipette is aligned with the hole of ● in FIG. When injecting samples in other rows, align the left end of the pipette with the corresponding numbered hole in FIG.
[0073]
At this time, the file number is added as shown in Table 6 after the character string of the sample name [fixed part].
[0074]
[Table 6]

[0075]
2) -2 When injecting with an 8 pipette (4)
“Numbering” is “(H1, G1, F1,..., C1, B1, A1)” “(H7, G7, F7,..., C7, B7, A7)”, and “Sample arrangement” is “A1: When “1, A2: 2, A3: 3,...” Is set, injection into the sample tray is performed in the same manner as 2) -1.
[0076]
The file name is appended as shown in Table 7 after the character string of the sample name [fixed part].
[0077]
[Table 7]

[0078]
3) When injecting one by one (1)
3) -1 When “Numbering” is set to “Continuous (01-48)”
(The same number is added to “Sample Sequence” regardless of which is selected.)
.., 47, 48 in order from the sample data on the left side of the sample tray.
[0079]
3) -2 When “Numbering” is set to “Continuous (49-96)”
(The same number is added to “Sample Sequence” regardless of which is selected.)
49, 50,... 95, 96 in order from the sample data on the left side of the sample tray.
[Brief description of the drawings]
FIG. 1 shows a schematic diagram of the overall configuration.
FIG. 2 shows a schematic diagram of a partial configuration of FIG.
FIG. 3 is a layout diagram of the main body (door open).
FIG. 4 shows an arrangement configuration diagram (door closing) of the main body.
FIG. 5 is a diagram illustrating the principle.
FIG. 6 is a plan view showing the arrangement of sample containers.
FIG. 7 is a configuration diagram showing a main configuration (hot plate opening) of the electrophoresis analyzer.
FIG. 8 is a configuration diagram showing a main configuration (hot plate closing) of the electrophoresis analyzer.
FIG. 9 shows an exploded view of the device unit.
FIG. 10 shows one drawing of the screen input device.
FIG. 11 shows a sample number setting diagram of a sample.
FIG. 12 shows a sample number setting diagram of a sample.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 ... Laser part, 2 ... DNA sequencer main-body part, 3 ... Screen input / output device, 4 ... Personal computer, 5 ... Keyboard, 11, 12 ... Laser, 13 ... Mirror, 14 ... Box, 15 ... Sample storage chamber part, 16 ... Sample chamber portion 17. Sample door 30. Table 32 Buffer buffer tank 34 Sample tray holder 40 Capillary 42 95 Depressor 60, 96 Sheath flow cell 66 Drain adapter 67 DESCRIPTION OF SYMBOLS ... Drain tube, 69 ... Drain bottle, 90 ... Base, 91 ... Vertical plate part, 92 ... Plane plate part, 93 ... Laser beam irradiation member, 94 ... Hot plate, 97 ... Stopper, 100 ... Sample tray, 100-1 ... Sample container, 102 ... Sample plate, 200 ... Earth, 201 ... Sheath liquid tube, 202, 203,204 ... Tube, 210 ... Plate comprising electrodes .

Claims (2)

In an electrophoretic analyzer for optically analyzing a plurality of sample components,
It has a pedestal composed of a vertical plate portion and a flat plate portion,
A group consisting of a sheath flow cell base having a sheath flow cell, a capillary combined with the sheath flow cell, a hot plate for controlling the temperature of the capillary, and a laser irradiation port member for irradiating the sheath flow cell with laser light is arranged in the vertical direction of the base. Attach to the plate,
A sample tray containing a buffer liquid tank and a sample is placed on a table, the table is slidably installed on the flat plate portion of the pedestal, and the group and table installed as described above together with the pedestal An electrophoretic analyzer characterized by being disposed in a sample chamber formed in an analyzer main body. The electrophoretic analyzer according to claim 1,
A sheath flow cell base comprising the sheath flow cell is mounted on a vertical plate part,
The capillary is mounted below the sheath flow cell base,
The hot plate is attached to the front of the capillary,
The laser irradiation port member is attached to one side of the sheath flow cell,
The laser beam of the laser irradiation port member is irradiated into the sheath flow cell,
Fluorescence generated in the sheath flow cell by irradiation with the laser light is detected by an optical sensor disposed behind the sheath flow cell.

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