JP2006176439A - Permanent wave agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To solve the problems of a conventional permanent wave agent wherein defects giving numerous damages to the hair and providing unpleasant smell and skin irritation when being used because the agent utilizes a two-step chemical reaction of cleaving the bond with a reducing agent under an alkali condition and recombining the cleaved bond with an oxidizing agent because the permanent wave is achieved by the cleavage and recombination of a disulfide bond in the hair. <P>SOLUTION: The permanent wave agent utilizes an enzyme of transglutaminase. As a result, the agent does not give the damage to the hair because the agent sets the hair in a waved or linear hair shape without using the reducing agent and the oxidizing agent nearly at neutral pH because the agent does not depend on the disulfide bond in the hair, which is different from the conventional agent for the permanent wave. The permanent wave agent does not cause the unpleasant smell and the irritation to the skin and is safe to a human body because of not using chemicals such as ammonia, thioglycolic acid and bromic acid. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention is a permanent agent that is easy to operate, does not damage the hair, has no unpleasant odor during use, can give a natural wave to the hair, and can make warped hair straight straight hair. It is about.

  In recent years, there has been a tendency to increase the chances of hair dyeing and perming, regardless of gender age, due to the improvement of fashion awareness for hair. In particular, the demand for hair dyeing is increasing not only for the fashionable dyeing of the younger generation, but also for gray hair as the population ages. However, such repeated hair dyeing and perming is highly likely to cause damage to the hair, and the user loses the moisture and luster of the hair due to the perm and hair coloring. Dissatisfaction that it has decreased is out. Therefore, the demand for permanents is declining as they damage hair. The mechanism of action of the current permanent agent is to set the shape that requires hair using a rod, etc., and then to promote the reduction reaction, disulfide bonds in the hair are thioglycolic acid, cysteine, etc. The sulfhydryl group is cleaved with a reducing agent, and then recombined with disulfide using an oxidizing agent such as hydrogen peroxide or sodium bromate to fix the hair shape. For this reason, the hair protein is denatured or washed away by chemicals such as reducing agents, alkaline agents and oxidizing agents used by hair proteins, and the hair is damaged.

  Accordingly, various permanent agents have been proposed for the purpose of solving consumer dissatisfaction with such permanent agents. For example, there is a patent (Patent Document 1) that uses cysteamine as a reducing agent. However, when cysteamine is used, there is an unpleasant odor, and since the molecular weight is small, there is a drawback that penetration into the skin becomes easy and irritation occurs. In addition, cysteamine is intended to prevent hair damage caused by permanent agents, but its mechanism of action depends on disulfide bonds in conventional hair, and the pH during use is also alkaline, so hair damage Prevention has not always reached a satisfactory level.

  On the other hand, transglutaminase is widely distributed in nature as an in vivo enzyme. In recent years, transglutaminase has been used to increase the viscosity of proteinaceous foods and improve the texture of products. As widely used in the food industry. In addition, regarding cosmetics, a patent (Patent Document 2) has been filed for improving hair damage caused by transglutaminase. However, the hair shape is altered by, for example, giving waves to the hair or straightening the distorted hair There are no reports on its use as a permanent agent.

JP-A-7-69846 JP-A-2-204407

  The present invention eliminates unpleasant odors during use without using hair-damaging agents such as alkaline agents, reducing agents, and oxidizing agents, such as conventional permanent agents dependent on disulfide bonds. It is an object to provide a permanent agent that is safe for the human body and has no skin irritation.

  As a result of intensive research on the above problems, the present inventors have made the enzyme transglutaminase act on the hair, without using a reducing agent or oxidizing agent, without damaging the hair, and being uncomfortable during use. It has been found that the shape of the hair can be changed as desired without odor, and the present invention has been completed.

  The permanent agent of the present invention will be described in detail. The transglutaminase (EC 2.3.2.13) used in the present invention is one of protein modifying enzymes, and it contains glutamine and lysine residues in proteins and peptides. It is known as an enzyme that forms a cross-link between them and binds proteins and peptides together. Therefore, if a new crosslink is formed by the present enzyme between the glutamine residue and the lysine residue on the hair surface and inside the hair, the hair can be freely deformed. This transglutaminase is known to exist in tissues of humans and mammals, organs, blood, as well as seafood and microorganisms, and the transglutaminase used in the present invention can be any one regardless of its origin. Those that can be used may be purified enzymes or extracts derived from tissues having enzyme activity.

  In addition, calcium ions are generally required for the reaction of transglutaminase, but depending on the type, there are also calcium-independent enzymes. Therefore, in the present invention, calcium ions are not always essential for the expression of enzyme functions, and it is desirable to add calcium ions as necessary.

  In addition, as described above, transglutaminase is widely used as a proteinaceous material or an enzyme agent for processing food in the food industry, and is recognized as an enzyme that is highly safe for the human body.

  Transglutaminase is sold as a reagent. In the food industry field, transglutaminase is commercially available as an enzyme agent for food processing and can be easily obtained.

Transglutaminase activity was measured by using benzyloxycarbonyl-L-glutaminylglycine and hydroxylamine as substrates, adding a necessary amount of calcium ion to an enzyme that requires calcium ion, and then reacting the resulting hydroxamic acid with trichloroacetic acid. It was formed as an iron complex in the presence, the absorbance at 525 nm was measured, and the amount of hydroxamic acid was calculated from the calibration curve. The activity of transglutaminase was measured by the method described below unless otherwise specified.
<Activity measurement method>
Reagent A
0.02M Tris-HCl buffer (pH 6.0)
0.1M Hydroxylamine 0.01M Reduced Curtathione 0.03M Benzyloxycarbonyl-L-glutaminylglycine Reagent B (1: 1: 1 mixture of the following solutions)
3M hydrochloric acid 12% trichloroacetic acid 5% ferric chloride (dissolved in 0.1M hydrochloric acid)
Measurement procedure Add 0.5 mL of reagent A to 0.05 mL of the enzyme solution, mix and react at 37 ° C. for 10 minutes, add 0.5 mL of reagent B to stop the reaction and form an iron complex, and then absorb the absorbance at 525 nm. taking measurement. As a control, the absorbance of an enzyme solution that has been reacted in the same manner using an enzyme solution that has been heat-inactivated in advance is measured, and the absorbance difference from the enzyme solution is determined. Separately, a calibration curve is prepared using L-glutamic acid γ-monohydroxamic acid instead of the enzyme solution, and the amount of hydroxamic acid generated from the difference in absorbance is determined. Enzymatic activity to produce 1 μmol hydroxamic acid per minute Was taken as 1 unit.

  The amount of transglutaminase to be added to the permanent agent depends on the form of the preparation, the frequency of use, and the treatment time, but usually 0.1 to 50,000 units, particularly 1 to 10,000 units, per 1 g of hair. It is preferable to do this. If the blending amount is less than 1 unit, the effect of the enzyme may not be exhibited, and if it exceeds 50,000 units, the effect corresponding to the increase in the amount of the enzyme cannot be expected.

  The pH at the time of using this permanent agent is usually 4 to 10, preferably 5 to 8. The reaction temperature when using the permanent agent is 10 to 80 ° C, preferably 30 to 50 ° C. The reaction time is usually 1 minute to 4 hours, preferably 10 minutes to 2 hours.

  In order to make the cross-linking formed by transglutaminase more robust, the cross-linking is enhanced by adding an appropriate amount of calcium salt, apart from the expression of the enzyme function. For example, calcium chloride, calcium acetate, calcium lactate, calcium citrate, calcium palmitate, calcium stearate, calcium gluconate, calcium carbonate, calcium oxide, and calcium hydroxide can be used. More preferably, when calcium chloride, calcium acetate or calcium lactate is used, the enhancement of crosslinking is significant. The addition amount is usually 0.1 to 100 mM, particularly 1 to 10 mM as the calcium concentration.

  Further, in order to strengthen and stabilize the ability to deform the hair formed with the present permanent agent, it is effective to further heat-treat after the treatment with the present permanent agent. The heat treatment is usually 30 to 80 ° C. for 1 minute to 2 hours, preferably 40 to 60 ° C. for 5 to 60 minutes.

  By treating the hair under the above conditions, the permanent agent of the present invention can impart straight hair without waves or wrinkles to the hair without damaging the hair.

  Moreover, in order to keep the transglutaminase in a formulation stable, an amino acid, a peptide, and protein can also be added individually or in combination, respectively.

  The permanent agent can be prepared in any form of preparation that can be applied to the hair, such as powder, solid, liquid, cream, gel, and the like. Specifically, it can be widely used as a dosage form for external use such as lotion, hair cream, hair lotion, milky lotion, ointment and the like. The liquid form can be used in combination with a normal propellant such as carbon dioxide gas, oxygen gas, LPG, etc., and filled in a pressure resistant container.

  Furthermore, in addition to the above essential components, this permanent agent contains other medicinal ingredients for maintaining healthy hair and promoting hair growth in general, such as vitamin A, vitamin B6, pantothene, etc. Vitamins such as acids and biotin, amino acids such as methionine, cysteine, and tyrosine, bactericides such as salicylic acid, hinokitiol, resorcin, and other hormones can be added as necessary. In addition, components usually used in these preparations such as oils, surfactants, moisturizers, lower alcohols, thickeners, antioxidants, chelating agents, feeling of use, pH adjusters, preservatives, perfumes, A pigment | dye etc. can be mix | blended and the addition amount of these medicinal components can be normally used in the range which does not inhibit the effect of this invention.

  The method of using the permanent agent of the present invention is to treat hair with a reducing agent under alkaline conditions after treating the hair into a desired shape using a rod or the like like a normal permanent agent, and further with an oxidizing agent 2 There is no need for a chemical reaction at the stage. After the hair is adjusted to the desired shape using a rod, etc. in the same way as a normal permanent agent, the permanent agent of the present invention is applied to the hair and left at the required temperature for an appropriate time. And then wash it off.

  As described above, since the permanent agent using the transglutaminase of the present invention does not depend on the disulfide bond in the hair unlike the conventional permanent agent, the hair is damaged without using a reducing agent and an oxidizing agent. Because its function is expressed at a pH value that does not give hair, it exhibits a natural wave or straight effect on the hair without damaging the hair. In addition, since no chemicals such as ammonia and cysteamine were used, a permanent agent with no unpleasant odor and no irritation to the skin was obtained.

  EXAMPLES Hereinafter, although an Example and a comparative example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to the following Example. In addition, the part of the compounding amount of each component shown in the examples indicates parts by weight, and% indicates% by weight.

Formulation Example 1 Powder
Formulation Formulation 1. Lactose 97 parts Transglutaminase (100,000 units / g) 2
3. Casein 1
Production method: Components 1 to 3 are uniformly mixed to obtain a product.

Usage 1
Wash 1g of commercially available human hair bundle with shampoo, wipe off moisture with a towel, attach to a permanent rod, dissolve 1g of powder in 4mL of water, apply it, and leave it at 40 ° C for 40 minutes. When the bundle is removed from the rod, washed with shampoo and air-dried, a hair bundle with a supple wave is obtained.

Usage 2
After washing 1 g of a commercially available human hair bundle with a shampoo, wipe off moisture with a towel, attach it to a perm rod, apply 1 g of the powder agent in 4 mL of an aqueous solution containing 10 mM calcium chloride, and apply at 40 ° C., 40 After leaving for a minute, the hair bundle is removed from the rod, washed with a shampoo, and then air-dried.

Usage 3
Wash 1g of commercially available human hair bundles with shampoo, wipe off moisture with a towel, attach to a permanent rod, dissolve 1g of powder in 4mL of water, apply, leave at 40 ° C for 40 minutes, The hair bundle is further heated at 50 ° C. for 10 minutes with the hair bundle attached thereto, and then the hair bundle is removed from the rod, washed with shampoo and then air-dried to obtain a hair bundle that is more strongly waved than in use method 1.

Usage 4
After washing 1 g of a commercially available human hair bundle with a shampoo, wipe off moisture with a towel, attach it to a perm rod, apply 1 g of the powder agent in 4 mL of an aqueous solution containing 10 mM calcium chloride, and apply at 40 ° C., 40 After leaving for a minute, after heating for 10 minutes at 50 ° C with the hair bundle attached to the rod, the hair bundle is removed from the rod, washed with shampoo and then air-dried. A bundle is obtained.

Formulation Example 2 Liquid formulation Formulation amount 1. Transglutaminase (1,000 units / g) 20 parts 2.1 M Triethanolamine / hydrochloric acid buffer (pH 7.0) 10
3. Methyl paraoxybenzoate 0.1
4). Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
5. Glycerin 10
6). Perfume appropriate amount 7. Making the total amount 100 parts with purified water Production method: Components 1, 2 and 7 and components 3 to 6 are uniformly dissolved separately, and both are mixed and filtered to obtain a product.

Usage 5
After washing 1 g of commercially available human hair bundle with shampoo, wipe off moisture with a towel, attach it to the permanent rod, apply 3 g of liquid, leave it at 40 ° C for 40 minutes, remove the hair bundle from the rod, and shampoo After washing and air-drying, a hair bundle with a supple wave is obtained.

Usage 6
After washing 1 g of a commercially available human hair bundle with a shampoo, wiping off moisture with a towel, attaching it to a perm rod, applying 10 mM calcium chloride dissolved in 3 g of the solution, leaving it at 40 ° C. for 40 minutes, When the bundle is removed from the rod, washed with a shampoo and then air-dried, a hair bundle that is more strongly waved than in Method 5 is obtained.

Usage 7
Wash 1g of commercially available human hair bundle with shampoo, wipe off moisture with a towel, attach it to the perm rod, apply 3g of liquid, leave it at 40 ° C for 40 minutes, and then keep the hair bundle attached to the rod. After heating at 50 ° C. for 10 minutes, the hair bundle is removed from the rod, washed with a shampoo and then air-dried to obtain a hair bundle that is stronger than the usage method 5.

Usage 8
Wash 1g of commercially available human hair bundle with shampoo, wipe off moisture with towel, attach to permanent rod, apply 10mM calcium chloride dissolved in 3g of solution, leave at 40 ° C for 40 minutes, then rod After further heating at 50 ° C. for 10 minutes with the hair bundle attached, the hair bundle is removed from the rod, washed with a shampoo and then air-dried to obtain a hair bundle that is more strongly waved than the usage methods 6 and 7. .

Formulation Example 3 Latex Formulation Formulation amount 1. Squalane 5.0 parts Olive oil 5.0
3. Jojoba oil 5.0
4). Cetanol 1.5
5. Glycerol monostearate 2.0
6). Polyoxyethylene cetyl ether (20E.O.) 3.0
7). Polyoxyethylene sorbitan monooleate 2.0
8). Perfume appropriate amount 9. Propylene glycol 1.0
10. Glycerin 2.0
11. Transglutaminase (1,000 units / g) 1.0
12 Methyl paraoxybenzoate 0.2
13. Making the total amount 100 parts with purified water Production method: Components 1 to 7 are heated and dissolved and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 9 to 13 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 8 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Usage 9
Wash 1g of commercially available human hair bundle with shampoo, wipe off moisture with a towel, attach it to the permanent rod, apply 3g of emulsion, leave it at 40 ° C for 40 minutes, remove the hair bundle from the rod, and shampoo After washing with air and air drying, a hair bundle with a supple wave is obtained.

Usage 10
After washing 1 g of a commercially available human hair bundle with a shampoo, wiping off moisture with a towel, attaching it to a perm rod, applying 10 mM calcium chloride dissolved in 3 g of an emulsion, leaving it at 40 ° C. for 40 minutes, When the hair bundle is removed from the rod, washed with a shampoo and then air-dried, a hair bundle that is more strongly waved than method 9 is obtained.

Usage 11
After washing 1g of commercially available human hair bundle with shampoo, wipe off moisture with a towel, attach it to the permanent rod, apply 3g of emulsion, leave it at 40 ° C for 40 minutes, and then keep the hair bundle attached to the rod Further, after heating at 50 ° C. for 10 minutes, the hair bundle is removed from the rod, washed with a shampoo and then air-dried.

Usage 12
After washing 1 g of a commercially available human hair bundle with a shampoo, wiping off moisture with a towel, attaching it to a perm rod, applying 10 mM calcium chloride dissolved in 3 g of an emulsion, leaving it at 40 ° C. for 40 minutes, After further heating at 50 ° C. for 10 minutes with the hair bundle attached to the rod, the hair bundle is removed from the rod, washed with a shampoo and then air-dried to obtain a hair bundle that is more strongly waved than use method 10 and use method 11. .

Comparative Example 1
Except for transglutaminase in Formulation Example 1, the same treatment is carried out, and the hair bundle is obtained by treatment according to the method of use method 1.

Comparative Example 2
For comparison, a permanent treatment with a normal permanent agent was performed.
Reducing agent A formulation Formulation amount 1. 50% ammonium thioglycolate 14 parts 2.40% ammonium dithioglycolate 2.5
3. 60% hydroxyethanediphosphonic acid 0.5
4). Purified water 50
5. Ammonia water appropriate amount 6. The total volume is made 100 parts with purified water.
Production method: Components 1 to 4 are mixed, pH is adjusted to 8.0 with Component 5, Component 6 is added so that the total amount becomes 100, and uniformly dissolved.
Oxidizing agent B formulation Sodium bromate 8.0 parts 2.60% hydroxyethane diphosphonic acid 0.5
3. Sodium citrate 0.45
4). Citric acid 0.05
5. The total volume is made 100 parts with purified water.
Production method: Components 3 and 4 are added to 5 and uniformly dissolved, and then components 1 and 2 are added to uniformly dissolve the entire amount.

    Usage: After washing 1g of commercially available human hair bundle with shampoo, wipe off moisture with a towel, attach it to the perm rod, apply 4g of reducing agent A, leave it for 15 minutes, wash with water, and then oxidize B After applying 4 g and treating for 10 minutes, the hair bundle is removed from the rod, washed with a shampoo, and then air-dried.

Usage test 1
With respect to the hair bundles obtained by carrying out the above-mentioned three kinds of prescription examples and two kinds of comparative examples, the wave power of the permanent, the damage to the hair, and the sustainability of the wave were evaluated. In addition, the usage method in each prescription example was implemented by the usage methods 1, 5, and 9. In addition, hair damage was evaluated by sliding due to the gloss of the hair and the touch of the hair, the wave force was visually confirmed, the wave persistence was brushed 30 times daily, and the retention of the wave after 30 days was visually confirmed. . The evaluation criteria are 7 levels: 1: not very good, 2: not good, 3: not good, 4: normal, 5: good, 6: good, 7: very good. It was shown in 1.

  From the above results, Prescription Examples 1 to 3 have a slightly weaker wave power and sustainability than Comparative Example 2 which is a normal perm agent, but the gloss and smoothness of the hair are good and do not damage the hair. It turns out that it is a permanent agent.

Usage test 2
In addition, a test for human use was carried out by 3 persons each using Formulation Example 2 Liquid Agent Usage Method 6 and Comparative Example 2 normal permanent agent. The results are shown in Table 2. In addition, evaluation was performed about the wave power of a permanent, the damage to hair, the sustainability of a wave, and the irritation | stimulation to a scalp. Damage to the hair was confirmed by the gloss and smoothness of the hair in the same manner as in Test 1. The evaluation criteria were evaluated in seven levels: 1: not very good, 2: not good, 3: not good, 4: normal, 5: good, 6: good, 7: very good.

  From the above results, it can be clearly seen that the present permanent agent has a slightly weaker wave force than the normal permanent agent, but does not damage the hair and is less irritating to human skin.

  A normal permanent agent damages hair because it uses a reducing agent and an oxidizing agent. Therefore, the development of a permanent agent that does not damage hair is desired by users of the permanent agent. This permanent agent does not use a reducing agent or an oxidizing agent, and acts in a neutral region that does not damage the hair. Therefore, it is possible to give the hair a natural wave or straight hair without damaging the hair. Therefore, there is a use as a permanent agent that can be used with peace of mind by users of permanent agents who are dissatisfied with hair damage and those who wish to use permanent agents who have been afraid of damaging their hair.

Claims (4)

  A permanent agent containing transglutaminase.   The permanent agent of Claim 1 containing a calcium salt.   The permanent agent according to claim 1 or 2, wherein 1 to 10,000 units of transglutaminase is added to 1 g of hair.   A permanent treatment method comprising using the permanent agent according to claim 1 and performing a heat treatment after the permanent treatment.