JP2006166907A5 - - Google Patents
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- Publication number
- JP2006166907A5 JP2006166907A5 JP2005327174A JP2005327174A JP2006166907A5 JP 2006166907 A5 JP2006166907 A5 JP 2006166907A5 JP 2005327174 A JP2005327174 A JP 2005327174A JP 2005327174 A JP2005327174 A JP 2005327174A JP 2006166907 A5 JP2006166907 A5 JP 2006166907A5
- Authority
- JP
- Japan
- Prior art keywords
- concentration
- poly
- reaction
- rna
- adapter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000006243 chemical reaction Methods 0.000 claims 12
- 238000000034 method Methods 0.000 claims 10
- 108020004414 DNA Proteins 0.000 claims 7
- 102000053602 DNA Human genes 0.000 claims 7
- 239000012472 biological sample Substances 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 230000006820 DNA synthesis Effects 0.000 claims 3
- 238000010839 reverse transcription Methods 0.000 claims 3
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 claims 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 claims 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 claims 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 claims 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 claims 2
- 229910001425 magnesium ion Inorganic materials 0.000 claims 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 1
- 108010083359 Antigen Receptors Proteins 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 1
- 210000001744 T-lymphocyte Anatomy 0.000 claims 1
- 210000003719 b-lymphocyte Anatomy 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims 1
- 238000007857 nested PCR Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005327174A JP4069133B2 (ja) | 2004-11-19 | 2005-11-11 | 生物試料に含まれる目的遺伝子を増幅する方法 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004336090 | 2004-11-19 | ||
| JP2005327174A JP4069133B2 (ja) | 2004-11-19 | 2005-11-11 | 生物試料に含まれる目的遺伝子を増幅する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2006166907A JP2006166907A (ja) | 2006-06-29 |
| JP2006166907A5 true JP2006166907A5 (OSRAM) | 2007-03-22 |
| JP4069133B2 JP4069133B2 (ja) | 2008-04-02 |
Family
ID=36668269
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005327174A Expired - Fee Related JP4069133B2 (ja) | 2004-11-19 | 2005-11-11 | 生物試料に含まれる目的遺伝子を増幅する方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4069133B2 (OSRAM) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009098988A1 (ja) * | 2008-02-04 | 2009-08-13 | Genecare Research Institute Co., Ltd. | siRNA検出方法 |
| CN106103711A (zh) | 2013-11-21 | 2016-11-09 | 组库创世纪株式会社 | T细胞受体和b细胞受体库分析系统及其在治疗和诊断中的应用 |
| US10533204B2 (en) | 2014-05-30 | 2020-01-14 | National University Corporatlon University of Toyama | Method for amplifying a T cell receptor (TCR) cDNA |
| CN109517889B (zh) | 2017-09-18 | 2022-04-05 | 苏州吉赛基因测序科技有限公司 | 一种基于高通量测序分析寡核苷酸序列杂质的方法及应用 |
| WO2021033648A1 (ja) * | 2019-08-20 | 2021-02-25 | 国立感染症研究所長 | ヌクレオチド配列の増幅方法及び配列決定方法 |
| CN113774496A (zh) * | 2021-10-11 | 2021-12-10 | 湖南大地同年生物科技有限公司 | 一种液相捕获文库构建方法 |
-
2005
- 2005-11-11 JP JP2005327174A patent/JP4069133B2/ja not_active Expired - Fee Related
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