JP2006121954A - Method for producing enzyme degradation product - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for producing an enzyme degradation product efficiently carrying out a process for producing the enzyme degradation product and reducing cost. <P>SOLUTION: The method for producing the enzyme degradation product is to enzymatically react a raw material to be the enzyme degradation product by jet flow agitation in an agitation apparatus. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a method for producing an enzyme degradation product, and more particularly to a method for producing an enzyme degradation product in which an enzyme reaction is carried out in a stirrer that jets and stirs a raw material to be an enzyme degradation product.

  Conventionally, as a method for producing an enzymatic degradation product, a stirring device that stirs a raw material that becomes an enzymatic degradation product with a stirring blade is known as an enzymatic decomposition reaction device. In such a stirring device, the raw material is stirred with a stirring blade. Since the agitation is performed, it takes a long time for the enzyme decomposition, and it is desired that an enzyme decomposition reaction apparatus appears in a short time. If the enzyme decomposition can be performed in a short time, the cost can be reduced.

  On the other hand, a stirring device that does not require a mechanical driving source such as a blade is known as a stirring device (Patent Document 1), and such a stirring device is good when stirring a highly viscous liquid. Since the stirring effect can be obtained, the present inventors have earnestly studied whether this stirring apparatus can be used as an enzymatic decomposition reaction apparatus.

If such a stirring device can be used as an enzyme decomposition reaction device, the production process of the enzyme decomposition product can be performed efficiently, and the cost can be reduced.
Japanese Patent No. 3058876

  An object of the present invention is to provide a method for producing an enzymatic degradation product that can be produced in a short time, reduce costs by increasing the efficiency of the production time, and obtain a high-quality enzymatic degradation product. .

  The method for producing an enzymatic degradation product according to the present invention is characterized in that the raw material to be the enzymatic degradation product is subjected to an enzymatic reaction by jet stirring in a stirrer.

  When the raw material in the stirrer is subjected to an enzyme reaction by jet stirring in the stirrer, the raw material circulates in the stirrer by jet stirring, and the raw material in the stirrer rotates, so the circulation efficiency is high and the jet itself is very Since it has a strong turbulence, the stirring efficiency in the stirring device is increased, so that the enzymatic degradation time can be shortened and the quality of the enzymatic degradation product is increased.

  The method for producing an enzymatic degradation product according to claim 2 is characterized in that the stirring device according to claim 1 has a jet part for jetting liquid or gas, and the jet stirring by jetting of the liquid or gas from the jet part causes the enzyme to It is characterized by reacting.

  When the liquid or gas is blown into the raw material in the stirring device to cause an enzymatic reaction, the raw material circulates in the stirring device by jet stirring, and the raw material in the stirring device rotates, so the circulation efficiency is higher, Since the jet itself has a very strong turbulence, the stirring efficiency in the stirring device is further increased, so that the enzymatic decomposition time can be further shortened and the quality of the enzymatic decomposition product is also increased.

  The method for producing an enzymatic degradation product according to claim 3 is characterized in that the raw material according to claim 1 is a protein raw material, a sugar raw material, a fiber, or an oil.

  In the method for producing an enzymatic degradation product according to claim 4, the jet part according to claim 2 is arranged such that one or a plurality of nozzles for injecting liquid or gas into the raw material are arranged in the container of the stirring device. Features.

  The method for producing an enzymatic degradation product according to claim 5 is characterized in that the liquid according to claim 2 is a raw material obtained by circulating the raw material in the stirring device.

  The method for producing an enzymatic degradation product according to claim 6 is characterized in that the gas according to claim 2 is air or an inert gas.

  When the raw material in the stirrer is subjected to an enzyme reaction by jet stirring in the stirrer, the raw material circulates in the stirrer by jet stirring, and the raw material in the stirrer rotates, so the circulation efficiency is high and the jet itself is very Since it has a strong turbulence, the stirring efficiency in the stirring device is increased, so that the enzymatic degradation time can be shortened and the quality of the enzymatic degradation product is increased.

  An embodiment according to the present invention will be described.

  In this example, a protein raw material is used as a raw material for the enzyme degradation product, and this protein raw material will be explained using a scallop membrane (scallop string) separated from a scallop built-in (scallop uro). It is not something that can be done.

  As a protein raw material, for example, waste discarded during processing of fish such as squid goro, byproducts of chicken internal organs, seafood in animal systems, beef, pork, chicken, milk casein, milk, etc., wheat in plant systems, In the case of cereals such as soybeans and fermentation systems, yeast can be used as a protein raw material as long as it contains protein.

  Moreover, if not only a protein raw material but a starch substance is contained, it can be used as a saccharide raw material.

  Furthermore, plants (fibrous, woody) can also be used as a raw material. For example, waste vegetables, sawdust chips, mushrooms and the like. Moreover, as a lipid, squid oil, vegetable oil, petroleum etc. can be used as a raw material, for example.

  Next, scallop string separated from scallops as a protein raw material is finely divided. The reason why the scallop string is made fine is to facilitate the enzymatic reaction in the stirrer and also to enable circulation to the stirrer.

  In this embodiment, the scallop string is finely ground as a paste.

  And the raw material which is this scallop string is liquefied, accommodated in a stirring apparatus, and an enzyme reaction is carried out in the stirring apparatus.

As shown in FIG. 1, the stirring device 1 includes a cylindrical container 2 having an inner diameter D, and a liquefied raw material B to be stirred is accommodated in the container 2.
The container 2 may be a container having a polygonal planar shape with an inscribed circle D.

The container 2 is provided with a nozzle 5 that is oriented upward at a depth H1 from the raw material surface and serves as a jetting part for blowing the raw material B that is a liquid into the raw material B.
This nozzle is provided upward, but it may be downward or sideways. The ejection part may be an ejection hole provided in the bottom wall 4 of the container 2.

A pump (not shown) is connected to the nozzle 5 so that the liquid supplied from the pump can be injected into the raw material B from the nozzle 5.
Although not shown, the container 2 is provided with a delivery pipe for delivering the raw material in the container 2 to the outside and ejecting the raw material from the nozzle 5 into the container.
That is, the raw material in the container 2 is sent to the outside and ejected from the nozzle 5 into the container so that the raw material circulates.

  The raw material B is stored in the container 2 so that the height from the bottom wall 4 of the container 2 to the raw material surface is H1 + H2, and the nozzle 5 is arranged upward from the raw material surface to a depth of H1.

The nozzle 5 has a ratio H1 / D between the depth H1 and the inner diameter or inscribed circle D in the range of 0.3 to 1.6, and the flow rate Q _{L of the} liquid blown from the nozzle 5 into the raw material B is Q _L ^2. / (Gd _ner ² D ³ ) = 0.01 (where g is the gravitational acceleration and d _nen is the inner diameter of the nozzle), and the flow rate is less than the flow rate at which the liquid jet does not blow out from the raw material.

The above is the condition of the jet in the case of liquid, but in the case of gas, the nozzle 5 has a ratio H1 / D between the depth H1 and the inner diameter or inscribed circle D in the range of 0.3 to 1. The flow rate Q _{a of the} gas blown from the nozzle 5 into the raw material B is equal to or higher than the flow rate satisfying ρLQ _a ² / (σLD ³ ) = 10 ⁻⁵ (where ρL is the density of the raw material and σL is the surface tension of the raw material). And it is below the flow volume which a gaseous bubble does not blow through to a raw material.

  In the nozzle 5, the ratio H1 / D between the depth H1 and the inner diameter or the inscribed circle D may be in the range of 0.3 to 1.6, but in the embodiment, the ratio H1 / D is 0.5.

  The container 2 of the agitator 1 shown in the figure includes a bottom wall 4 and a side wall 3, and a nozzle 5 is provided at the center of the container 5, but a plurality of nozzles may be provided.

The stirrer is not limited to the stirrer described above, and has a jet part that jets liquid or gas to the raw material in the stirrer, and performs an enzyme reaction by jet stirring of the raw material by jetting liquid or gas from the jet part It is only necessary to be able to perform the enzyme reaction of the raw material to be the enzyme decomposition product by jet stirring in the stirring device.
Moreover, although the raw material which is a liquid is circulated in the above, you may make it jet-stir by ejecting the gas from a nozzle to a raw material, without circulating a raw material.
When jetting gas, an air compressor is used instead of the pump used in the case of liquid.

  Enzymatic reaction is carried out in this stirrer, but the enzyme reaction in the stirrer differs depending on the raw material, and in this example, scallops are used as the raw material. Therefore, ThermosePC-10 (Daiwa Kasei) is used as the protease. used.

  Enzymes vary depending on the raw material, and amylase is used for carbohydrate raw materials, cellulase is used for fibrous raw materials, and lipase is used for fat and oil raw materials that are lipids.

  In this example, the enzyme was added to scallop 2% by weight (protein weight ratio), added with the same amount of water as scallop, and reacted at 70 ° C. for 24 hours.

  After completion of the reaction, heating is performed at 90 ° C. for 30 minutes to deactivate the enzyme. The inactivated reaction solution was centrifuged, and the supernatant was collected and suction filtered using a filter aid to obtain a filtrate.

  In the above example, 2% by weight (protein weight ratio) of enzyme was added to scallop, which is a protein raw material, but it may be 0.5% to 3% by weight.

  Further, if the raw material is a cellulase for fiber decomposition, it is 0.1% to 1.0% by weight relative to the raw material weight, and as a saccharide raw material amylase, for example, dry starch is used as the raw material, 0.1% weight to 2.0%. % Weight is sufficient.

  In this example, an equal amount of water was added to the protein raw material and reacted at 70 ° C. for 24 hours. However, the water addition may be 0 to 5 times, and the amount of water that the raw material can be stirred in the stirrer is sufficient. I just need it.

  The reaction temperature and reaction time are not limited to the above, and may be 1 to 48 hours and 20 to 80 ° C.

  In the above, after completion of the reaction, heating is performed at 90 ° C. for 30 minutes to deactivate the enzyme. The inactivated reaction solution is centrifuged, and the supernatant is collected and suction filtered using a filter aid, and then the filtrate is obtained as a product. However, the reaction solution obtained by enzymatic degradation can also be used as the product.

  This filtrate is used as a product, but if necessary, it can be concentrated and dried to obtain a product.

  The filtrate which is an enzyme degradation product by the production method of the above embodiment will be described.

  In addition, a comparison is a comparison with the filtrate in the case of the stirring apparatus (displayed as a jet) by this manufacturing method, and the filtrate in the case of the stirring apparatus (impeller type) by the conventional stirring blade.

  First, regarding the time course of the protein in the filtrate, as shown in FIG. 2, the protein content of the obtained filtrate was measured by Kjeldahl analysis.

  The protein content increases with this production method, and the difference increases with time. This means that according to this production method, the protein recovery rate is high, the production time of the enzyme reaction product can be greatly shortened, and the production cost can be increased.

  Next, the change over time in the amount of free amino acid in the filtrate is compared with that in the conventional production method as shown in FIG.

  There are free amino acids in the form of protein in the filtrate, and the properties (functionality, flavor) of the filtrate vary depending on the content.

  As shown in Fig. 3, in the filtrate by this production method, the maximum ratio was reached in 7 hours, and it was found that even if the enzymatic degradation time was shortened to this time, the nature of the extract, especially the flavor, was not affected. Even if the time is shortened, the properties of the filtrate do not change, and the quality of the product can be maintained at a high quality even if the production time is short.

  Next, the time-dependent change in ACE (angiotensin I converting enzyme) inhibitory activity (blood pressure increase inhibitory effect) in the filtrate is compared with that in the conventional production method as shown in FIG.

  The smaller this value is, the stronger the ACE inhibitory activity is. However, as shown in FIG. 4, according to this production method, the ACE inhibitory activity is maximized in 9 hours. In the filtrate according to the present production method, the ACE inhibitory activity can be maximized in a short time, so that a functional degradation product can be obtained efficiently.

When the raw material in the container is sent to the outside, the raw material is circulated, and the raw material is blown into the container from the nozzle 5 of the stirring device 1, the raw material stays in the container in the conventional method, so the raw material is sterilized. In this case, the raw material can be sterilized by circulation, and the efficiency of the manufacturing process can be improved as compared with the conventional manufacturing method.
Furthermore, by circulating, even if the raw material has a slightly larger particle size, the pulverization effect can be obtained by the circulation, and the enzymatic degradation ability is improved.

It is a side view of the stirring apparatus of one Example which concerns on this invention. It is explanatory drawing of the time-dependent change of the protein in the filtrate by this manufacturing method. It is explanatory drawing of the time-dependent change of the amount of free amino acids in the filtrate by this manufacturing method. It is explanatory drawing of the time-dependent change of the ACE (angiotensin I converting enzyme) inhibitory activity (blood pressure rise inhibitory effect) in the filtrate by this manufacturing method.

Explanation of symbols

1 Stirrer 2 Container 5 Nozzle

Claims (6)

A method for producing an enzymatic degradation product, wherein the raw material to be an enzymatic degradation product is subjected to an enzymatic reaction by jet stirring in a stirrer. 2. The enzyme degradation product according to claim 1, wherein the stirring device has a jet part for jetting liquid or gas, and is subjected to an enzyme reaction by jet stirring by jetting liquid or gas from the jet part. Manufacturing method. The said raw material is a protein raw material, a saccharide raw material, a fiber, and a lipid, The manufacturing method of the enzyme degradation product of Claim 1 characterized by the above-mentioned. 3. The method for producing an enzymatic degradation product according to claim 2, wherein the jetting part is provided with one or a plurality of nozzles for blowing liquid or gas into the raw material in a container of a stirring device. The method for producing an enzymatic degradation product according to claim 2, wherein the liquid is a raw material obtained by circulating a raw material in a stirring device. The method for producing an enzymatic degradation product according to claim 2, wherein the gas is air or an inert gas.

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