JP2006081472A - Method for producing new germinated brown rice and the resultant germinated brown rice - Google Patents

Method for producing new germinated brown rice and the resultant germinated brown rice Download PDF

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JP2006081472A
JP2006081472A JP2004269929A JP2004269929A JP2006081472A JP 2006081472 A JP2006081472 A JP 2006081472A JP 2004269929 A JP2004269929 A JP 2004269929A JP 2004269929 A JP2004269929 A JP 2004269929A JP 2006081472 A JP2006081472 A JP 2006081472A
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brown rice
germinated brown
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gaba
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Naganori Daihisa
長範 大久
Hiroyuki Nagasato
浩之 長里
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KOMACHI NOGYO KYODO KUMIAI
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KOMACHI NOGYO KYODO KUMIAI
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for producing new brown rice rich in Gaba(γ-aminobutyric acid) with a relatively simple operation and in a short time. <P>SOLUTION: The method for producing new germinated brown rice is characterized by involving keeping moderately pounded and refined brown rice or powder thereof at 35-45°C for 4 h or longer. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

この発明は、玄米の発芽を利用して、玄米中にグルタミン酸を適度に保ちながら精製するγ−アミノ酪酸の含有量を増大させることを目的とした新しい発芽玄米の製造方法に関する。   The present invention relates to a new method for producing germinated brown rice for the purpose of increasing the content of γ-aminobutyric acid, which is purified while keeping glutamic acid in the brown rice by using germination of brown rice.

γ−アミノ酪酸(以下Gabaと略記する)は神経抑制作用や精神安定機能を有しており、血圧上昇抑制作用、脳の代謝促進作用、動脈硬化の予防、二日酔い防止、皮膚の老化防止などに効果のある機能性食品成分として注目されている。そのためGabaを富化させる方法が種々検討されており、米糠や胚芽を浸漬し富化する方法(J.Agric Food Chem、42、1122、1994年)、グルタミン酸を酵母やクロレラに作用させる方法(特開平9−238650)、お茶を嫌気的に醗酵させる方法(日本農芸化学会誌、61、817、1987年)、大豆もやしを炭酸ガスで処理する方法(食科工、36、916、1989年)、麹菌によるもの(特開平10−165191)、乳酸菌を活用する方法(Biosci Biotech Biochem、61、1168、1997年)等が報告されている。また、玄米を高圧処理することによりGabaが増加することも報告されている(食品科学工学会誌、46、323、1999年)。   γ-Aminobutyric acid (hereinafter abbreviated as Gaba) has a neurosuppressive effect and a tranquilizing function, and is effective in suppressing blood pressure increase, promoting brain metabolism, preventing arteriosclerosis, preventing hangover, preventing skin aging, etc. It is attracting attention as an effective functional food ingredient. For this reason, various methods for enriching Gaba have been studied. Methods for immersing and enriching rice bran and germ (J. Agric Food Chem, 42, 1122, 1994), methods for causing glutamic acid to act on yeast and chlorella (specialized) Kaihei 9-238650), a method of anaerobically fermenting tea (Journal of Japanese Society for Agricultural Chemistry, 61, 817, 1987), a method of treating soybean sprouts with carbon dioxide (shokugan, 36, 916, 1989), A method using a gonococcus (Japanese Patent Laid-Open No. 10-165191), a method using a lactic acid bacterium (Biosci Biotech Biochem, 61, 1168, 1997) and the like have been reported. It has also been reported that Gaba increases by high-pressure treatment of brown rice (Journal of Food Science and Engineering, 46, 323, 1999).

また、注目すべき発芽玄米の製造方法及び栄養強化玄米としては、以下のようなものがある。   Moreover, there are the following as a method for producing germinated brown rice to be noted and nutrient-enriched brown rice.

(1)水に溶解したミネラル類やビタミン類を発芽過程において吸収させる栄養強化玄米の発明。   (1) Invention of nutrient-enriched brown rice that absorbs minerals and vitamins dissolved in water during the germination process.

(2)オゾンを含む空気を間欠的に曝気して温水を殺菌しながら発芽する方法の発明。   (2) Invention of a method for germination while intermittently aeration of air containing ozone to sterilize hot water.

(3)焼成カキ殻カルシウム製剤、ゼオライト及び活性炭を共存させた層で濾過しながら玄米を発芽させる方法の発明。
特開2000−50818 特開平11−4661 特開2000−321100
(3) Invention of a method for germinating brown rice while filtering through a layer in which a calcined oyster shell calcium preparation, zeolite and activated carbon coexist.
JP2000-50818 JP-A-11-4661 JP 2000-321100 A

日常の食生活で高血圧などの生活習慣病が予防できれば消費者のメリットは大きい。その一つとして、発芽玄米から適量のGabaを摂取する方法が考えられる。しかし、玄米を30℃前後で保温した場合、Gabaの含量を高めるためには、浸漬した状態で,70時間もの発芽時間を必要とした(秋田県総合食品研究所報告、1、85〜86頁、1999年)。そこで発芽に必要な設備を最小限に絞り、かつ玄米のGabaの含量を高めるための技術開発が求められていた。   Consumers benefit greatly if they can prevent lifestyle-related diseases such as high blood pressure in their daily diet. As one of them, a method of taking an appropriate amount of Gaba from germinated brown rice can be considered. However, when brown rice was kept at around 30 ° C., germination time as long as 70 hours was required to increase the content of Gaba (Akita Prefectural Food Research Institute report, pages 1, 85-86). 1999). Therefore, there has been a demand for technological development to minimize facilities necessary for germination and increase the content of Gaba in brown rice.

この発明は、適度に搗精した玄米を35℃〜45℃の温水に入れて4時間以上保温することにより、Gabaの高収量を得て、前記従来の問題点を解決したのである。   In the present invention, moderately refined brown rice is placed in warm water at 35 ° C. to 45 ° C. and kept warm for 4 hours or more, thereby obtaining a high yield of Gaba and solving the conventional problems.

即ちこの発明は、適度に搗精した玄米又はその粉末を35℃〜45℃で4時間以上保温することを特徴とした新しい発芽玄米の製造方法であり、適度の搗精とは、搗精歩合3%〜5%としたこものであり、保温時間は5時間〜16時間としたものである。   That is, the present invention is a new method for producing germinated brown rice characterized by keeping moderately refined brown rice or its powder at 35 ° C. to 45 ° C. for 4 hours or more. The heat retention time is 5 to 16 hours.

また他の発明は、適度に搗精した玄米又はその粉末を35℃〜45℃で4時間以上保温して得た発芽玄米である。   Another invention is germinated brown rice obtained by warming moderately refined brown rice or its powder at 35 ° C. to 45 ° C. for 4 hours or more.

この発明において37℃においては、保温時間4時間までは急激に増加し、爾後緩徐に増加し、20時間以上増加を続ける(図2)。   In the present invention, at 37 ° C., it rapidly increases until the incubation time is 4 hours, slowly increases after sputum, and continues to increase for 20 hours or more (FIG. 2).

また温度を50℃にした場合には、4時間まではGaba量が増加するが、8時間以上保温してもほぼ同等のGaba量を示し、グルタミン酸量も一定である。   When the temperature is 50 ° C., the amount of Gaba increases up to 4 hours. However, even if the temperature is kept for 8 hours or more, the amount of Gaba is almost the same, and the amount of glutamic acid is also constant.

前記発明において、保温温度を35℃〜45℃にしたのは、図1に示すように、この時間帯が最も収量が多いからである。   In the present invention, the heat retention temperature is set to 35 ° C. to 45 ° C., as shown in FIG. 1, because this time zone has the highest yield.

この発明において、搗精歩合を3%〜5%としたのは、表1に示すように、Gabaの収量が多くなるからであり、表1には示されていないが、搗精歩合1%〜3%又は5%〜6%でも保温温度と時間の関係で相当の収量を示すので、搗精歩合は1%〜6%であるが、好ましくは3%〜5%とした。   In the present invention, the reason why the fine rate is 3% to 5% is that, as shown in Table 1, the yield of Gaba increases, and although not shown in Table 1, the fine rate is 1% to 3%. % Or 5% to 6% shows a considerable yield due to the relationship between the temperature and the temperature, and the fineness ratio is 1% to 6%, preferably 3% to 5%.

従来Gabaについて相当の収量をあげるには70時間もの保温を必要としたが、この発明によれば4時間〜20時間、好ましくは5時間〜16時間でGabaの大きな収量を得ることができる効果がある。   Conventionally, in order to increase the yield of Gaba, a heat retention of 70 hours was required. However, according to the present invention, it is possible to obtain a large yield of Gaba in 4 hours to 20 hours, preferably 5 hours to 16 hours. is there.

この発明は、搗精歩合3%の玄米を、37℃で6時間〜12時間保温した所、Gaba300nmol/ml〜480nmol/mlを得た。   This invention obtained Gaba 300 nmol / ml-480 nmol / ml, when the brown rice of 3% of the milling ratio was kept at 37 ° C. for 6 hours to 12 hours.

この発明の実施例を説明すれば、搗精歩合3%、5%、10%の玄米(あきたこまち(登録商標))0.5gに、0.1M酢酸緩衝液(pH6.0)1mlを加え、37℃に保温した。保温後、1NHCl0.5mlを添加し、反応を停止した。   Explaining an example of the present invention, 1 ml of 0.1 M acetate buffer (pH 6.0) was added to 0.5 g of brown rice (Akitakomachi (registered trademark)) 3%, 5%, and 10% of the milling ratio, and 37 Incubated at 0 ° C. After the incubation, 0.5 ml of 1N HCl was added to stop the reaction.

ついで超音波とブレンダーで1分間混合した後、この混合液を遠心分離(3000rpm、10分間)し、上澄水を得た。この上澄水を0.45μmのミソポアフィルターで濾過し、濾過済み試料を自動アミノ酸分析機(日本電子、JLC−500V)によりGabaおよびグルタミン酸を定量した所、表1の結果を得た。

Figure 2006081472
Subsequently, after mixing for 1 minute with an ultrasonic wave and a blender, this mixed solution was centrifuged (3000 rpm, 10 minutes) to obtain a supernatant. The supernatant water was filtered through a 0.45 μm misopore filter, and the filtered sample was quantified for Gaba and glutamic acid using an automatic amino acid analyzer (JEOL, JLC-500V). The results shown in Table 1 were obtained.
Figure 2006081472

前記実施例1における搗精歩合を3%とし、グルタミン酸を添加し、保温時間を12時間として、温度を変化させた所、図1の結果を得た。   The results shown in FIG. 1 were obtained by changing the temperature by setting the fine fraction in Example 1 to 3%, adding glutamic acid, and keeping the incubation time to 12 hours.

即ち搗精歩合3%の場合には、37℃程度の温度が最良であった。   That is, when the fineness ratio was 3%, a temperature of about 37 ° C. was the best.

実施例1における搗精歩合を3%とし、グルタミン酸を添加し、温度を37℃として、保温時間を変化させた所、4時間〜12時間で、Gabaの増加が著しいことが判明した。   It was found that the increase in Gaba was remarkable in 4 hours to 12 hours when the kneading ratio in Example 1 was 3%, glutamic acid was added, the temperature was 37 ° C., and the incubation time was changed.

この発明の実施例における好ましい温度を示すグラフ。The graph which shows the preferable temperature in the Example of this invention. 同じく37℃における保温時間とGaba等の収量を示すグラフ。The graph which similarly shows the heat retention time in 37 degreeC, and yields, such as Gaba. 同じく50℃における保温時間とGaba等との収量を示すグラフ。The graph which similarly shows the heat retention time in 50 degreeC, and the yield of Gaba etc.

Claims (4)

適度に搗精した玄米又はその粉末を35℃〜45℃で4時間以上保温することを特徴とした新しい発芽玄米の製造方法。   A method for producing a new germinated brown rice, characterized in that moderately refined brown rice or a powder thereof is kept at 35 ° C to 45 ° C for 4 hours or more. 適度の搗精とは、搗精歩合3%〜5%としたことを特徴とする請求項1記載の新しい発芽玄米の製造方法。   The method for producing a new germinated brown rice according to claim 1, wherein the appropriate milling is 3% to 5%. 保温時間は5時間〜16時間としたことを特徴とする請求項1記載の新しい発芽玄米の製造方法。   The method for producing new germinated brown rice according to claim 1, wherein the heat retention time is 5 to 16 hours. 請求項1記載の方法により製造したことを特徴とする発芽玄米。   A germinated brown rice produced by the method according to claim 1.
JP2004269929A 2004-09-16 2004-09-16 Method for producing new germinated brown rice and the resultant germinated brown rice Pending JP2006081472A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015107069A (en) * 2013-12-04 2015-06-11 汎 伊藤 Method for producing sprouted brown rice
CN106819851A (en) * 2017-02-17 2017-06-13 江南大学 Nutrition reinforced type sprouted unpolished rice compounding instant-rice and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003088316A (en) * 2001-06-18 2003-03-25 Fancl Corp Unpolished rice
US20040105921A1 (en) * 2002-11-27 2004-06-03 Sang-You Kim Method for preparing germinated brown rice having improved texture and cookability without microbial contamination and a germinated brown rice obtained therefrom
JP2004159617A (en) * 2002-11-15 2004-06-10 Sunstar Inc METHOD FOR PRODUCING SPROUTED BROWN RICE HAVING INCREASED gamma-AMINOBUTYRIC ACID CONTENT AND FOOD CONTAINING THE SPROUTED BROWN RICE

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003088316A (en) * 2001-06-18 2003-03-25 Fancl Corp Unpolished rice
JP2004159617A (en) * 2002-11-15 2004-06-10 Sunstar Inc METHOD FOR PRODUCING SPROUTED BROWN RICE HAVING INCREASED gamma-AMINOBUTYRIC ACID CONTENT AND FOOD CONTAINING THE SPROUTED BROWN RICE
US20040105921A1 (en) * 2002-11-27 2004-06-03 Sang-You Kim Method for preparing germinated brown rice having improved texture and cookability without microbial contamination and a germinated brown rice obtained therefrom

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015107069A (en) * 2013-12-04 2015-06-11 汎 伊藤 Method for producing sprouted brown rice
CN106819851A (en) * 2017-02-17 2017-06-13 江南大学 Nutrition reinforced type sprouted unpolished rice compounding instant-rice and preparation method thereof
CN106819851B (en) * 2017-02-17 2020-05-26 江南大学 Nutrition-enhanced germinated brown rice compound instant rice and preparation method thereof

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