JP2006016364A - External composition for skin and food - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a safe, stable and inexpensive external composition for skin and food effectively ameliorating roughened skin or blackening of the skin induced by an oxidizing reaction and an inflammatory reaction caused by ultraviolet light, etc. <P>SOLUTION: The external composition for skin and food are obtained by formulating an extract prepared by carrying out solvent extraction from a seed of Durio spp. including Durio zibethinus Murr., Durio oxleyanus and Durio graveolens, especially the solvent extraction with an extracting solvent selected from water, a water-soluble organic solvent or a mixed solvent thereof. The external composition for skin and food have excellent effects on ameliorating roughened skin or blackening of skin caused by the ultraviolet light and are safe, stable and inexpensive. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a composition for external use for skin and a food, and more particularly, an extract obtained from durian seeds, in particular, an extract obtained using water, a water-soluble organic solvent or a mixed solvent thereof as an extraction solvent. The present invention relates to a composition for external use on skin and a food having excellent antioxidant ability, anti-inflammatory effect and whitening effect.

  The human body is affected in various ways by exposure to ultraviolet rays. When active oxygen, lipid peroxide, and the like are generated, they cause inflammation and cause great damage to tissues such as skin. Further, these inflammations and skin damage cause rough skin, which causes loss of moisture, gloss and texture of the skin, and when the effect reaches the dermis, it causes photoaging such as wrinkles. In addition, active oxygen generated by ultraviolet rays and various factors released from epidermal cells activate tyrosinase of melanocytes and overproduce melanin. Excess melanin is delivered to epidermal cells, changing the skin tone and leading to skin darkening. Other ultraviolet rays may cause skin cancer through DNA damage.

  For the purpose of preventing and improving various skin problems such as rough skin, photoaging, pigmentation, etc. caused by ultraviolet rays, conventionally, cosmetics, pharmaceuticals, foods, etc. have been used for dibutylhydroxytoluene (BHT), butylhydroxyanisole. Synthetic antioxidants such as (BHA) and various antioxidants such as naturally derived α-tocopherol and ascorbic acid are used (for example, see Patent Documents 1 and 2). However, the effect of preventing and improving skin troubles caused by exposure to ultraviolet rays is not sufficient, and there is a current situation that the antioxidant property does not necessarily lead to improvement of skin troubles caused by ultraviolet rays. Furthermore, the former synthetic antioxidant has a concern in terms of safety, and the latter has many problems in terms of stability.

In addition, various antioxidant substances extracted from plant seeds have been manufactured and tried to be formulated into cosmetics, pharmaceuticals, foods, etc. When using plants as raw materials, procurement of the raw materials, production costs, etc. From the standpoint of production, the production amount is limited, and the production cost is expensive. Therefore, it is difficult to blend an effective amount substantially.
JP-A-5-320036 JP-A-9-263513

  Under such circumstances, the object of the present invention is to provide a safe, stable and inexpensive external composition for skin that is excellent in the effect of improving rough skin and skin blackening caused by oxidation reaction and inflammation reaction caused by ultraviolet rays and the like. And providing food.

  As a result of diligent research in view of the above circumstances, the present inventors have contained various active ingredients having an antioxidant effect and an anti-inflammatory effect in extracts obtained from durian seeds that are normally discarded, When this extract was blended into a composition for external use of skin or food, it was found that it has a remarkable effect in improving rough skin and skin blackening caused by ultraviolet rays. Moreover, they are naturally derived substances, have no safety problems, and have excellent stability.

  That is, the first invention of the present application lies in an external composition for skin characterized by blending an extract obtained by solvent extraction from durian seeds. 2nd invention exists in said skin external composition characterized by the extraction solvent being chosen from water, a water-soluble organic solvent, or these mixed solvents. A third invention resides in a food characterized by blending an extract obtained by solvent extraction from durian seeds. 4th invention exists in said foodstuff whose extraction solvent is chosen from water, a water-soluble organic solvent, or these mixed solvents.

  In the present invention, by incorporating an extract obtained from durian seed into a composition for external use of skin or food, it is excellent in the effect of preventing rough skin and skin blackening caused by skin damage caused by ultraviolet rays and the like. Thus, it is possible to provide a stable and inexpensive external composition for skin and food.

Hereinafter, embodiments of the present invention will be described in detail. Durian (Durio zibethinus
Murr. Durio spp., Including Durio oxleyanus, Durio graveolens. ) Is a Panaceae plant that originates from Borneo to West Malaysia and is widely distributed in Southeast Asia. This durian fruit is said to be the king of fruits and is one of the fine tropical fruits. The fruit is about the size of a human head and is spherical or oval, with sharp and hard thorns growing on the entire skin. The fruit is divided into bunches, and each bundle contains seeds wrapped in pale yellow pulp. The pulp can be eaten as is, but the seeds are usually discarded. Other than eating raw, it is also sold as processed foods such as durian paste, durian ice and durian chips. In this case, the seeds separated in the processing process are discarded. Therefore, these seeds can be obtained at a very low price.

  In the present invention, the extraction solvent used for solvent extraction can be any known extraction solvent used for solvent extraction from plant materials. As the extraction solvent, a solvent having a relatively high hydrophilicity is preferably used because an effective extract that meets the object of the present invention can be obtained. As such a solvent, in the present invention, a solvent selected from water, a water-soluble organic solvent or a mixed solvent thereof is particularly preferably used. The water-soluble organic solvent refers to a solvent having compatibility with water, specifically, lower alcohols such as methanol, ethanol, propanol, isopropanol and butanol, polyhydric alcohols such as butylene glycol and propylene glycol, and acetic acid. Examples thereof include aliphatic carboxylic acid esters such as methyl and ethyl acetate, and other highly polar organic solvents such as acetone. Among the extraction solvents, an ethanol aqueous solution is preferable.

  In the extraction, first, the durian seed is washed, and then the seed is shredded and crushed by a known appropriate means. Extraction with a solvent may be performed according to a known method. For example, when water, a water-soluble organic solvent, or a mixed solvent thereof is used as an extraction solvent, the extraction solvent amount is 1 to 50 times, preferably 3 to 10 times, 0 to 100 ° C. What is necessary is just to extract for 0.1 to 50 hours, preferably 0.5 to 24 hours under the temperature conditions in the range of ° C and lower than the boiling point of the extraction solvent. Although the extraction may be in a stationary state, it is desirable to perform the extraction with moderate stirring for more efficient extraction.

  The durian seed extract thus obtained may be blended in the composition for external use of skin and food as it is, or may be blended after being concentrated or dried. Moreover, you may use what re-extracted what was extracted with the other organic solvent etc. Furthermore, a fraction obtained by fractionating and purifying the extract by a known fractionation means or purification means may be used.

  The composition for external use on skin and food containing the durian seed extract of the present invention can be various pharmaceuticals, quasi drugs, cosmetics, foods, health foods and the like. The dosage form is not particularly limited, and various forms can be used. Specifically, ointments, lotions, emulsions, creams, packs, granules, cataplasms, and the like can be used. Cosmetics are not particularly limited as long as they are applicable to human skin, and are cosmetics such as skin cosmetics, foundations, makeup cosmetics such as lipsticks, hair cosmetics such as shampoos and rinses, treatments, bath preparations, facial cleansers. It is possible to use various things. In addition, the food can be in various forms such as tablets, pills, dragees, powders, granules, teas, tea bags, hard capsules, soft capsules, strawberries, ice, baked goods.

  The amount of durian seed extract added to the composition for external use on skin and food according to the present invention is not particularly limited, and since it varies depending on the purpose and the target, it cannot be specified unconditionally. However, generally it can be used at 0.00001 to 5.0 mass% in terms of dry matter.

  Moreover, the composition for external use and the food of the present invention can be combined with various components that are usually used within a range that does not impair the effect, if necessary. For example, in the case of cosmetics, oils, surfactants, humectants, thickeners, preservatives, antioxidants, ultraviolet absorbers, ultraviolet scattering agents, fragrances, dyes, pigments and the like. In the case of food, it is an excipient, an additive, a binder, a thickener, an emulsifier, a colorant, a fragrance, a food additive, a seasoning and the like.

EXAMPLES Hereinafter, although this invention is explained in full detail based on an Example and a comparative example, this invention is not limited by these. Moreover, unless otherwise specified,% shown in an Example and a comparative example means the mass%.
Production Example 1
The skin and flesh were removed from two mature durian fruits to obtain seeds (26 pieces, 424.6 g). A suitable total of 210.6 g was selected from these seeds, the surface was washed, shredded with a knife, and then crushed with a mixer. Crushing with a mixer was performed by adding 1000 ml of 50% ethanol. This crushed material was extracted as it was with stirring at room temperature for 24 hours, and then filtered to obtain durian seed extract 1 (752 g, 19.6 g in terms of solid content).
Production Example 2
After adding 100 ml of 99.5% ethanol to 100 ml (94 g) of durian seed extract of Production Example 1, centrifugation was performed to separate the supernatant portion and the precipitate portion. The supernatant was taken out (168 g, 2.14 g in terms of solid content), and this was used as durian seed extract 2.
Production Example 3
The precipitation part of Production Example 2 was collected and dried to obtain durian seed extract 3 (0.4 g).
(Measurement of antioxidant power)
The antioxidant ability of durian seed extract was measured according to the following method.
Liquid A: 0.5 mmol / L DPPH (1,1-Diphenyl-2-picrylhydrazyl, Wako Pure Chemical Industries) ethanol solution B liquid: 100 mmol / L Tris-HCl aqueous solution (pH 7.4)
C liquid (sample): durian seed extract 2 of Production Example 2
Procedure: Take 1.0 ml of liquid A, 0.8 ml of liquid B, and 0.2 ml of liquid C in a test tube, stir with a vortex mixer for about 10 seconds, and then leave it to stand at room temperature for 20 minutes, then use a spectrophotometer. 517
Absorbance A ₅₁₇ at nm was measured. As a control, the same amount of 75% ethanol solution was added instead of liquid C, and the same amount of ethanol was added instead of liquid A for the purpose of correcting the absorbance derived from the measurement sample itself, The antioxidant power value was determined according to the following equation. As a comparison of the antioxidant power, a 1 mmol / L ascorbic acid solution prepared with a 75% ethanol solution was used instead of the C solution.
Formula: [A ₅₁₇ (control)-{A ₅₁₇ (sample) -A ₅₁₇ (correction)}] / A ₅₁₇ (control) × 100 (%)
The measurement results are shown in Table 1. As can be seen from Table 1, the antioxidant power of the durian seed extract of the present invention was comparable to that of ascorbic acid.
[Table 1]
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Sample Antioxidant power (%)
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Durian Seed Extract of Example 2 88.75
Ascorbic acid (control) 75.99
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Examples 1 to 3 (rough skin improvement effect)
The effect of the composition for external use containing the durian seed extract of the present invention on the skin of ultraviolet rays when applied to the skin of hairless mice was examined by the following test method. The animals were 5 groups of 9 hairless mice aged 9 weeks at the start of the test.
1. Ultraviolet irradiation and rough skin evaluation method Ultraviolet irradiation was performed by irradiating UVB region ultraviolet rays in an amount of about 7 times the minimum amount of erythema. The degree of rough skin caused by ultraviolet irradiation was evaluated by the fluctuation of transdermal moisture transpiration (TEWL). TEWL was measured using a hydrograph before sample application, before UV irradiation, and after 3 or 4 days of irradiation. A value obtained by dividing the value 3 days or 4 days after irradiation by the value before the sample application was calculated as the TEWL fluctuation rate, and this value was used as an index of the degree of rough skin.
2. Sample and Experimental Method Samples (Examples 1 to 3) were prepared by blending Dorian seed extracts 1 to 3 obtained in Production Examples 1 to 3 at a predetermined concentration using a 50% ethanol aqueous solution as a base. First, 0.05 ml of these samples were applied to the skin of the back test site of hairless mice once a day for 7 days (preliminary application). The day after the final pre-application, UV irradiation was performed, and the sample was applied the next day after irradiation and 2 days after the irradiation. Table 2 shows the effect of the application of each sample on the rough skin of the hairless mouse.
[Table 2]
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Specimen TEWL Fluctuation Rate ――――――――――――――――――――――――――――――――――――
Example 1 (Dorian Seed Extract 1 1.0%) 2.63 (3 days after irradiation)
Comparative Example 1 (base only) 3.14 (3 days after irradiation)

Example 2 (Dorian Seed Extract 2 2.0%) 5.52 (4 days after irradiation)
Example 3 (Dorian Seed Extract 3 0.04%) 4.69 (4 days after irradiation)
Comparative Example 2 (base only) 6.33 (4 days after irradiation)
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In this test system, the smaller the TEWL fluctuation rate value, the more the UV rough skin is suppressed. From the comparison with the comparative example of this test, it can be seen that rough skin caused by ultraviolet rays is improved by applying the composition for external application containing a durian seed extract. In addition, the fluctuation | variation of TEWL by prior application was not seen.
Example 4 (Ultraviolet pigmentation inhibitory effect)
The effect of the composition for external use containing the durian seed extract of the present invention on ultraviolet pigmentation when applied to the skin of colored guinea pigs was examined by the following test method. The animals used were 8 A-1 guinea pigs per group, 6 weeks old at the start of the test.
1. Ultraviolet irradiation and pigmentation evaluation method The back skin was removed, a test site of 4 cm ² (2 × 2 cm) was set, and UVB region ultraviolet irradiation was performed. The amount of irradiation at one time was about 1.5 times the minimum amount of erythema, and irradiation was performed on the first, fourth and seventh days of the test. Pigmentation was evaluated using skin lightness (L ^* value) and skin lightness decrease (ΔL ^* value) as indices. Skin lightness was measured using a color difference meter CR-300 (manufactured by Minolta).
2. Sample and Experimental Method A sample (Example 4) containing 2.0% of durian seed extract 2 obtained in Production Example 2 was prepared using propylene glycol / ethanol / water (20:63:17) as a base. 0.1 ml of this sample was applied once a day for 2 weeks continuously for 2 weeks after the first ultraviolet irradiation. Skin brightness was measured before and after 2 weeks of UV irradiation. Table 3 shows the effect of guinea pigs on ultraviolet pigmentation by the application of the sample.
[Table 3]
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Sample L ^* value ΔL ^* value ――――――――――――――――――――――――――――――――――――
Example 4 (Dorian Seed Extract 2 2.0%) 57.05-5.86
Comparative Example 3 (base only) 56.25-6.51
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In this test system, the larger the L ^* value and the smaller the absolute value of the ΔL ^* value, the more the ultraviolet pigmentation was suppressed. From the results of this test, it is understood that pigmentation due to ultraviolet rays is suppressed by application of a composition for external application containing a durian seed extract.
Examples 5 to 7 (human use test)
Test samples (Examples 5 to 7) having the compositions shown in Table 4 below were prepared, and the effect on ultraviolet pigmentation when applied to human skin was examined by the same test method as described above.
[Table 4]
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Ingredients Example 5 Example 6 Example 7
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Ethanol 5.0 5.0 5.0
Polyoxyethylene monolaurate 0.3 0.3 0.3
Sorbitan (20E.O.)
Methyl paraoxybenzoate 0.1 0.1 0.1
Durian Seed Extract 1 (Production Example 1) 1.0
Durian Seed Extract 2 (Production Example 2) 2.0
Durian Seed Extract 3 (Production Example 3) 0.04
Purified water Residual amount Residual amount Residual amount ――――――――――――――――――――――――――――――――――――
(Mixing amount is mass%)
As a result of the test, it was found that these test samples can suppress pigmentation due to ultraviolet rays. In addition, no side effects such as itching and dryness were observed during the test. In addition, these test samples were placed in a constant temperature bath at 40 ° C. and left for one month, but no precipitate was formed or discolored, and the stability was excellent.

The present invention can provide cosmetics, food and drink, etc. as a composition for external use on skin and food. Examples thereof are given below, but the present invention is not limited to these examples. In addition, a compounding quantity is the mass%.
Example 8 (skin lotion)
Ingredient name Blending amount A component (1) Olive oil 10.0
(2) Isopropyl myristate 1.0
(3) Monolauric acid 0.5
Polyoxyethylene (20) sorbitan (4) Propylene glycol 1.0
(5) Glycerin 2.0
Component B (6) Durian Seed Extract 2 (Production Example 2) 5.0
(6) Methylparaben 0.1
(7) Ethanol 7.0
(8) Remaining amount of purified water After each component A and component B were uniformly dissolved, the components A and component B were mixed, stirred and dispersed, and then filled into a container. During use, the contents were uniformly shaken and dispersed.
Example 9 (skin cream)
Ingredient name Compounding amount A component (1) dense wax 25.0
(2) Stearic acid 5.0
(3) Stearyl alcohol 5.0
(4) Reduced lanolin 2.0
(5) Squalene 2.0
(6) Monostearate sorbitan 3.0
Monostearate (7) Polyoxyethylenesorbitan 3.0
(8) Propylene glycol 5.0
Component B (9) Durian Seed Extract 1 (Production Example 1) 5.0
(10) Methylparaben 0.2
(11) Remaining amount of purified water After each component A and component B were heated and dissolved, the mixture was stirred and cooled to 30 ° C. to produce a skin cream.
Example 10 (tablets)
Component name Blending amount (1) Durian seed extract 2 (Production Example 2) 5.0
(2) Dextrin 63.0
(3) Sodium ascorbate 20.0
(4) Starch 5.5
(5) Silicon dioxide 3.0
(6) Glycerin fatty acid ester 1.5
(7) Fragrance 1.0
(8) Crystalline cellulose 1.0
Each of the above components was uniformly mixed, and a tablet was produced by a conventional method.
Example 11 (granule)
Ingredient Name Formulation (1) Durian Seed Extract 1 5.0
(2) Sodium ascorbate 60.0
(3) Dextrin 15.0
(4) Starch 10.0
(5) Lactose 9.0
(6) Crystalline cellulose 1.0
The above ingredients were mixed and ground uniformly, and granules were produced by a conventional method.
Example 12 (jelly preparation)
Component Name Blending amount (1) Durian Seed Extract 1 (Production Example 1) 5.0
(2) Dextrin 29.0
(3) Palatinose 5.0
(4) Gelatin 1.0
(5) Pectin 0.5
(6) Inositol 5.0
(7) Citric acid 0.8
(8) Ascorbic acid 3.0
(9) Nicotinamide 0.01
(10) Purified water remaining amount The above components were mixed and a jelly preparation was produced by a conventional method.
Example 13 (chewing gum)
Component Name Blending amount (1) Durian seed extract 3 (Production Example 3) 0.2
(2) Gum base 30.0
(3) Maltitol 45.0
(4) Mannit 20.0
(5) Ascorbic acid 3.0
(6) Sorbitol 5.0
(7) Fragrance 1.0
(8) Purified water remaining amount The above components were mixed and chewing gum was produced by a conventional method.

  According to the present invention, a safe, stable and inexpensive external composition for skin and food containing dorian seed extract is provided, and rough skin and skin caused by skin damage due to oxidation reaction, inflammation reaction, etc. caused by ultraviolet rays etc. It becomes possible to improve the blackening of the skin from the outside by the composition for external use of the skin and from the inside of the body by the food.

Claims (4)

An external composition for skin, comprising an extract obtained by solvent extraction from durian seeds. The external composition for skin according to claim 1, wherein the extraction solvent is selected from water, a water-soluble organic solvent, or a mixed solvent thereof. A food comprising an extract obtained by solvent extraction from durian seeds. The food according to claim 3, wherein the extraction solvent is selected from water, a water-soluble organic solvent, or a mixed solvent thereof.