JP2005525114A - 全血からのｍＲＮＡをハイスループットに定量するための装置および方法 - Google Patents

全血からのｍＲＮＡをハイスループットに定量するための装置および方法 Download PDF

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JP2005525114A
JP2005525114A JP2003587943A JP2003587943A JP2005525114A JP 2005525114 A JP2005525114 A JP 2005525114A JP 2003587943 A JP2003587943 A JP 2003587943A JP 2003587943 A JP2003587943 A JP 2003587943A JP 2005525114 A JP2005525114 A JP 2005525114A
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Japan
Prior art keywords
mrna
quantification
quantified
blood
whole blood
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Pending
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JP2003587943A
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English (en)
Japanese (ja)
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JP2005525114A5 (zh
Inventor
マサト ミツハシ
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Showa Denko Materials Co Ltd
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Hitachi Chemical Co Ltd
Showa Denko Materials Co Ltd
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Application filed by Hitachi Chemical Co Ltd, Showa Denko Materials Co Ltd filed Critical Hitachi Chemical Co Ltd
Publication of JP2005525114A publication Critical patent/JP2005525114A/ja
Publication of JP2005525114A5 publication Critical patent/JP2005525114A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2003587943A 2002-04-24 2003-04-24 全血からのｍＲＮＡをハイスループットに定量するための装置および方法 Pending JP2005525114A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37547202P 2002-04-24 2002-04-24
PCT/US2003/012895 WO2003091407A2 (en) 2002-04-24 2003-04-24 Device and method for high-throughput quantification of mrna from whole blood

Publications (2)

Publication Number Publication Date
JP2005525114A true JP2005525114A (ja) 2005-08-25
JP2005525114A5 JP2005525114A5 (zh) 2006-06-15

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JP2003587943A Pending JP2005525114A (ja) 2002-04-24 2003-04-24 全血からのｍＲＮＡをハイスループットに定量するための装置および方法

Country Status (4)

Country Link
EP (1) EP1504121A4 (zh)
JP (1) JP2005525114A (zh)
CN (1) CN1650027B (zh)
WO (1) WO2003091407A2 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009538148A (ja) * 2006-05-24 2009-11-05 ジェン−プロウブ インコーポレイテッド 試験サンプルにおけるＭｙｃｏｂａｃｔｅｒｉｕｍｔｕｂｅｒｃｕｌｏｓｉｓ複合体生物の存在を決定するためのプローブおよびキット、ならびに捕捉プローブを使用するグラム陽性桿菌または真菌の増幅方法
JP2010534328A (ja) * 2007-07-23 2010-11-04 クロンデイアグ・ゲーエムベーハー アッセイ
JP2014508295A (ja) * 2011-02-17 2014-04-03 ネステク ソシエテ アノニム 白血球及び腫瘍細胞を濾過により単離するための装置及び方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050208501A1 (en) * 2004-03-16 2005-09-22 Ambion, Inc. Process and reagents for extraction of RNA from fractionated blood leukocytes
WO2006116721A1 (en) * 2005-04-28 2006-11-02 Hitachi Chemical Research Center, Inc. Ex vivo gene expression in whole blood as a model of assessment of individual variation to dietary supplements
CN106119382B (zh) * 2016-07-19 2018-08-28 湖州高亿诺生物科技有限公司 自动化微生物分子检测方法及其应用
CN106190817B (zh) * 2016-07-19 2019-01-08 温州市高亿诺生物科技有限公司 基于多孔板的核酸裂解液分离方法和装置

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US625229A (en) * 1899-05-16 Machine for forging horseshoe-nails or other articles
JPH0824583B2 (ja) * 1989-05-09 1996-03-13 株式会社ニッショー Ｄｎａの抽出方法
GB9220773D0 (en) * 1992-10-02 1992-11-18 Mini Agriculture & Fisheries Detection of antigens and nucleic acids
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
NO180658C (no) * 1994-03-10 1997-05-21 Oeystein Fodstad Fremgangsmåte og anordning for deteksjon av spesifikke målceller i spesialiserte eller blandede cellepopulasjoner og opplösninger som inneholder blandede cellepopulasjoner
US5976797A (en) * 1996-12-20 1999-11-02 Hitachi Chemical Co., Ltd. Method for quantifying total mRNA with poly(A)-complementary oligonucleotide-immobilized microtiter plate
EP1042497B1 (en) * 1997-12-22 2008-07-16 Hitachi Chemical Co., Ltd. Direct rt-pcr on oligonucleotide-immobilized pcr microplates
AU6114199A (en) * 1998-10-09 2000-05-01 Whatman Bioscience Limited Isolation method for nucleic acid and apparatus
WO2000077253A1 (fr) * 1999-06-16 2000-12-21 Hitachi, Ltd. Appareil et procede d'examen des genes
US6670128B2 (en) * 2000-08-16 2003-12-30 Whatman, Inc. Transfusion medicine leukodepletion filter devices as a source of genetic material for genotyping studies

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009538148A (ja) * 2006-05-24 2009-11-05 ジェン−プロウブ インコーポレイテッド 試験サンプルにおけるＭｙｃｏｂａｃｔｅｒｉｕｍｔｕｂｅｒｃｕｌｏｓｉｓ複合体生物の存在を決定するためのプローブおよびキット、ならびに捕捉プローブを使用するグラム陽性桿菌または真菌の増幅方法
JP2013106617A (ja) * 2006-05-24 2013-06-06 Gen-Probe Inc 試験サンプルにおけるＭｙｃｏｂａｃｔｅｒｉｕｍｔｕｂｅｒｃｕｌｏｓｉｓ複合体生物の存在を決定するためのプローブおよびキット、ならびに捕捉プローブを使用するグラム陽性桿菌または真菌の増幅方法
JP2010534328A (ja) * 2007-07-23 2010-11-04 クロンデイアグ・ゲーエムベーハー アッセイ
JP2014508295A (ja) * 2011-02-17 2014-04-03 ネステク ソシエテ アノニム 白血球及び腫瘍細胞を濾過により単離するための装置及び方法
KR101511889B1 (ko) * 2011-02-17 2015-04-13 네스텍 소시에테아노님 여과에 의해 백혈구 및 종양 세포를 단리하는 장비 및 방법
JP2016029378A (ja) * 2011-02-17 2016-03-03 ネステク ソシエテ アノニム 白血球及び腫瘍細胞を濾過により単離するための装置及び方法
US9535052B2 (en) 2011-02-17 2017-01-03 Nestec S.A. Apparatus and method for isolating leukocytes and tumor cells by filtration

Also Published As

Publication number Publication date
WO2003091407A2 (en) 2003-11-06
EP1504121A2 (en) 2005-02-09
CN1650027A (zh) 2005-08-03
EP1504121A4 (en) 2005-06-29
CN1650027B (zh) 2010-04-21
WO2003091407A3 (en) 2004-12-16

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