JP2005515757A - ミトコンドリア生物学発現アレイ - Google Patents
ミトコンドリア生物学発現アレイ Download PDFInfo
- Publication number
- JP2005515757A JP2005515757A JP2003524534A JP2003524534A JP2005515757A JP 2005515757 A JP2005515757 A JP 2005515757A JP 2003524534 A JP2003524534 A JP 2003524534A JP 2003524534 A JP2003524534 A JP 2003524534A JP 2005515757 A JP2005515757 A JP 2005515757A
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- sample
- array
- sequences
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000002438 mitochondrial effect Effects 0.000 title claims abstract description 110
- 230000014509 gene expression Effects 0.000 title claims abstract description 105
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 209
- 239000000523 sample Substances 0.000 claims abstract description 155
- 238000000034 method Methods 0.000 claims abstract description 63
- 241000282414 Homo sapiens Species 0.000 claims abstract description 52
- 230000004962 physiological condition Effects 0.000 claims abstract description 34
- 150000007523 nucleic acids Chemical class 0.000 claims description 67
- 238000009396 hybridization Methods 0.000 claims description 64
- 108020004707 nucleic acids Proteins 0.000 claims description 63
- 102000039446 nucleic acids Human genes 0.000 claims description 63
- 239000000758 substrate Substances 0.000 claims description 21
- 239000002773 nucleotide Substances 0.000 claims description 17
- 125000003729 nucleotide group Chemical group 0.000 claims description 17
- 102100032891 Superoxide dismutase [Mn], mitochondrial Human genes 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 9
- 238000007639 printing Methods 0.000 claims description 9
- 102100033053 Glutathione peroxidase 3 Human genes 0.000 claims description 7
- 101710119049 Glutathione peroxidase 3 Proteins 0.000 claims description 7
- 102000002812 Heat-Shock Proteins Human genes 0.000 claims description 7
- 108010004889 Heat-Shock Proteins Proteins 0.000 claims description 7
- 102000046669 Surf-1 Human genes 0.000 claims description 6
- 230000009467 reduction Effects 0.000 claims description 6
- 101150081019 surf1 gene Proteins 0.000 claims description 6
- 230000037149 energy metabolism Effects 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 108010045815 superoxide dismutase 2 Proteins 0.000 claims description 3
- 239000013074 reference sample Substances 0.000 claims description 2
- 208000006136 Leigh Disease Diseases 0.000 claims 2
- 208000017507 Leigh syndrome Diseases 0.000 claims 2
- 206010058799 Mitochondrial encephalomyopathy Diseases 0.000 claims 2
- 102100026396 ADP/ATP translocase 2 Human genes 0.000 claims 1
- 101000718417 Homo sapiens ADP/ATP translocase 2 Proteins 0.000 claims 1
- 101150079293 VDAC4 gene Proteins 0.000 claims 1
- 238000003491 array Methods 0.000 abstract description 7
- 239000003814 drug Substances 0.000 abstract description 6
- 230000008238 biochemical pathway Effects 0.000 abstract description 3
- 229940124597 therapeutic agent Drugs 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 88
- 241000699666 Mus <mouse, genus> Species 0.000 description 67
- 108020005196 Mitochondrial DNA Proteins 0.000 description 58
- 230000015572 biosynthetic process Effects 0.000 description 48
- 241000699670 Mus sp. Species 0.000 description 39
- 101150005399 sod2 gene Proteins 0.000 description 28
- 210000001519 tissue Anatomy 0.000 description 28
- 230000035772 mutation Effects 0.000 description 26
- 230000010627 oxidative phosphorylation Effects 0.000 description 24
- 238000002493 microarray Methods 0.000 description 23
- 230000001105 regulatory effect Effects 0.000 description 23
- 238000003752 polymerase chain reaction Methods 0.000 description 22
- 238000004458 analytical method Methods 0.000 description 21
- 238000011282 treatment Methods 0.000 description 19
- 108020004414 DNA Proteins 0.000 description 18
- 230000035790 physiological processes and functions Effects 0.000 description 17
- 238000011835 investigation Methods 0.000 description 16
- 230000000694 effects Effects 0.000 description 15
- 108020004999 messenger RNA Proteins 0.000 description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 14
- 210000003205 muscle Anatomy 0.000 description 14
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 13
- 210000003470 mitochondria Anatomy 0.000 description 13
- 208000012268 mitochondrial disease Diseases 0.000 description 13
- 102000004169 proteins and genes Human genes 0.000 description 13
- 230000004044 response Effects 0.000 description 13
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 12
- 239000002299 complementary DNA Substances 0.000 description 12
- 210000002216 heart Anatomy 0.000 description 12
- 238000012163 sequencing technique Methods 0.000 description 12
- 241000894007 species Species 0.000 description 12
- 230000000295 complement effect Effects 0.000 description 11
- 238000003745 diagnosis Methods 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 11
- 201000010099 disease Diseases 0.000 description 10
- 239000012634 fragment Substances 0.000 description 10
- 210000000952 spleen Anatomy 0.000 description 10
- 238000000018 DNA microarray Methods 0.000 description 9
- 241000282412 Homo Species 0.000 description 9
- 108091093105 Nuclear DNA Proteins 0.000 description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 229920000728 polyester Polymers 0.000 description 9
- 239000007787 solid Substances 0.000 description 9
- 208000011580 syndromic disease Diseases 0.000 description 9
- 239000002253 acid Substances 0.000 description 8
- 210000004556 brain Anatomy 0.000 description 8
- 230000008859 change Effects 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 210000003734 kidney Anatomy 0.000 description 8
- 238000010606 normalization Methods 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 238000011160 research Methods 0.000 description 8
- 238000003786 synthesis reaction Methods 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 7
- 108091034117 Oligonucleotide Proteins 0.000 description 7
- 230000032683 aging Effects 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- OAWZTKNCHQQRKF-UHFFFAOYSA-L manganese(3+);4-[10,15,20-tris(4-carboxyphenyl)porphyrin-22,24-diid-5-yl]benzoic acid Chemical compound [Mn+3].C1=CC(C(=O)O)=CC=C1C(C1=CC=C([N-]1)C(C=1C=CC(=CC=1)C(O)=O)=C1C=CC(=N1)C(C=1C=CC(=CC=1)C(O)=O)=C1C=CC([N-]1)=C1C=2C=CC(=CC=2)C(O)=O)=C2N=C1C=C2 OAWZTKNCHQQRKF-UHFFFAOYSA-L 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 230000007246 mechanism Effects 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical class NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 102000015930 Lon proteases Human genes 0.000 description 6
- -1 NDL4 Proteins 0.000 description 6
- 108010023294 Protease La Proteins 0.000 description 6
- 239000013614 RNA sample Substances 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 6
- 230000006907 apoptotic process Effects 0.000 description 6
- 238000004422 calculation algorithm Methods 0.000 description 6
- 206010012818 diffuse large B-cell lymphoma Diseases 0.000 description 6
- 230000007613 environmental effect Effects 0.000 description 6
- 230000002414 glycolytic effect Effects 0.000 description 6
- 210000004185 liver Anatomy 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 230000004060 metabolic process Effects 0.000 description 6
- 150000008300 phosphoramidites Chemical class 0.000 description 6
- 102000054765 polymorphisms of proteins Human genes 0.000 description 6
- 108091033319 polynucleotide Proteins 0.000 description 6
- 102000040430 polynucleotide Human genes 0.000 description 6
- 239000002157 polynucleotide Substances 0.000 description 6
- 238000000513 principal component analysis Methods 0.000 description 6
- 239000003642 reactive oxygen metabolite Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 108020004635 Complementary DNA Proteins 0.000 description 5
- 208000031671 Large B-Cell Diffuse Lymphoma Diseases 0.000 description 5
- 241000282577 Pan troglodytes Species 0.000 description 5
- 101710119418 Superoxide dismutase [Mn] Proteins 0.000 description 5
- 101710202572 Superoxide dismutase [Mn], mitochondrial Proteins 0.000 description 5
- 102000004962 Voltage-dependent anion channels Human genes 0.000 description 5
- 108090001129 Voltage-dependent anion channels Proteins 0.000 description 5
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 5
- 230000000735 allogeneic effect Effects 0.000 description 5
- 238000009826 distribution Methods 0.000 description 5
- 239000007850 fluorescent dye Substances 0.000 description 5
- 230000006698 induction Effects 0.000 description 5
- 208000015122 neurodegenerative disease Diseases 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 210000002027 skeletal muscle Anatomy 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 206010064571 Gene mutation Diseases 0.000 description 4
- 239000004472 Lysine Substances 0.000 description 4
- 108010029485 Protein Isoforms Proteins 0.000 description 4
- 102000001708 Protein Isoforms Human genes 0.000 description 4
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 4
- 230000006518 acidic stress Effects 0.000 description 4
- 230000037354 amino acid metabolism Effects 0.000 description 4
- 238000010171 animal model Methods 0.000 description 4
- 239000003963 antioxidant agent Substances 0.000 description 4
- 230000002715 bioenergetic effect Effects 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 238000007405 data analysis Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 208000035475 disorder Diseases 0.000 description 4
- 230000007935 neutral effect Effects 0.000 description 4
- 230000037361 pathway Effects 0.000 description 4
- 230000000241 respiratory effect Effects 0.000 description 4
- 230000002441 reversible effect Effects 0.000 description 4
- 238000002560 therapeutic procedure Methods 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 230000014616 translation Effects 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- 102100032533 ADP/ATP translocase 1 Human genes 0.000 description 3
- 229940088872 Apoptosis inhibitor Drugs 0.000 description 3
- 206010003591 Ataxia Diseases 0.000 description 3
- 102000014914 Carrier Proteins Human genes 0.000 description 3
- 108010078791 Carrier Proteins Proteins 0.000 description 3
- 108090000426 Caspase-1 Proteins 0.000 description 3
- 101000768061 Escherichia phage P1 Antirepressor protein 1 Proteins 0.000 description 3
- 108700039887 Essential Genes Proteins 0.000 description 3
- 102000005731 Glucose-6-phosphate isomerase Human genes 0.000 description 3
- 108010070600 Glucose-6-phosphate isomerase Proteins 0.000 description 3
- 101000796932 Homo sapiens ADP/ATP translocase 1 Proteins 0.000 description 3
- 108010086428 NADH Dehydrogenase Proteins 0.000 description 3
- 102000006746 NADH Dehydrogenase Human genes 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- 108020004566 Transfer RNA Proteins 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 239000000158 apoptosis inhibitor Substances 0.000 description 3
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 3
- 230000008436 biogenesis Effects 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 239000013068 control sample Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000004129 fatty acid metabolism Effects 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 238000003205 genotyping method Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 230000004898 mitochondrial function Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 210000004940 nucleus Anatomy 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 230000007170 pathology Effects 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 150000002978 peroxides Chemical class 0.000 description 3
- 238000001243 protein synthesis Methods 0.000 description 3
- 230000007129 protein targeting to mitochondrion Effects 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 230000035939 shock Effects 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 3
- 229940045145 uridine Drugs 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 102100021921 ATP synthase subunit a Human genes 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 2
- 206010003694 Atrophy Diseases 0.000 description 2
- 208000031229 Cardiomyopathies Diseases 0.000 description 2
- 102100035904 Caspase-1 Human genes 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 102100039327 Enoyl-[acyl-carrier-protein] reductase, mitochondrial Human genes 0.000 description 2
- 101710183457 Enoyl-[acyl-carrier-protein] reductase, mitochondrial Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 108060002716 Exonuclease Proteins 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 102000004300 GABA-A Receptors Human genes 0.000 description 2
- 108090000839 GABA-A Receptors Proteins 0.000 description 2
- 102000007390 Glycogen Phosphorylase Human genes 0.000 description 2
- 108010046163 Glycogen Phosphorylase Proteins 0.000 description 2
- 102100032510 Heat shock protein HSP 90-beta Human genes 0.000 description 2
- 101710163596 Heat shock protein HSP 90-beta Proteins 0.000 description 2
- 101000753741 Homo sapiens ATP synthase subunit a Proteins 0.000 description 2
- 101000636705 Homo sapiens NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Proteins 0.000 description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
- 206010025323 Lymphomas Diseases 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 208000029578 Muscle disease Diseases 0.000 description 2
- 102100031919 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Human genes 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 241000282579 Pan Species 0.000 description 2
- 108091093037 Peptide nucleic acid Proteins 0.000 description 2
- 108010050808 Procollagen Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 2
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000007792 addition Methods 0.000 description 2
- 229960000643 adenine Drugs 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 230000001640 apoptogenic effect Effects 0.000 description 2
- 230000037444 atrophy Effects 0.000 description 2
- 210000003719 b-lymphocyte Anatomy 0.000 description 2
- 210000000133 brain stem Anatomy 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 210000003850 cellular structure Anatomy 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 210000003483 chromatin Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- NIUVHXTXUXOFEB-UHFFFAOYSA-N coproporphyrinogen III Chemical compound C1C(=C(C=2C)CCC(O)=O)NC=2CC(=C(C=2C)CCC(O)=O)NC=2CC(N2)=C(CCC(O)=O)C(C)=C2CC2=C(C)C(CCC(O)=O)=C1N2 NIUVHXTXUXOFEB-UHFFFAOYSA-N 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
- 238000007418 data mining Methods 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 102000013165 exonuclease Human genes 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 150000003278 haem Chemical class 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 108091064355 mitochondrial RNA Proteins 0.000 description 2
- 230000026326 mitochondrial transport Effects 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 101150087123 nat gene Proteins 0.000 description 2
- 201000001119 neuropathy Diseases 0.000 description 2
- 230000007823 neuropathy Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 208000033808 peripheral neuropathy Diseases 0.000 description 2
- 238000000206 photolithography Methods 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 230000009919 sequestration Effects 0.000 description 2
- 230000019491 signal transduction Effects 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 150000003431 steroids Chemical class 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 229940126585 therapeutic drug Drugs 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 101150084750 1 gene Proteins 0.000 description 1
- DHFODCCTNFXCCQ-UHFFFAOYSA-N 1-methylpyrrolidine-2,3-dione Chemical compound CN1CCC(=O)C1=O DHFODCCTNFXCCQ-UHFFFAOYSA-N 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- SAUCHDKDCUROAO-UHFFFAOYSA-N 2-amino-3-oxobutanoic acid Chemical compound CC(=O)C(N)C(O)=O SAUCHDKDCUROAO-UHFFFAOYSA-N 0.000 description 1
- 102100031126 6-phosphogluconolactonase Human genes 0.000 description 1
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 1
- 108010017236 ATP Translocases Mitochondrial ADP Proteins 0.000 description 1
- 102100029344 ATP synthase protein 8 Human genes 0.000 description 1
- 102000023805 ATP:ADP antiporter activity proteins Human genes 0.000 description 1
- 101710126783 Acetyl-hydrolase Proteins 0.000 description 1
- 208000010444 Acidosis Diseases 0.000 description 1
- 108010009924 Aconitate hydratase Proteins 0.000 description 1
- 108010001058 Acyl-CoA Dehydrogenase Proteins 0.000 description 1
- 102000002735 Acyl-CoA Dehydrogenase Human genes 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 108010024976 Asparaginase Proteins 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102100026596 Bcl-2-like protein 1 Human genes 0.000 description 1
- 101100097220 Caenorhabditis elegans sft-4 gene Proteins 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 102000004046 Caspase-2 Human genes 0.000 description 1
- 108090000552 Caspase-2 Proteins 0.000 description 1
- 208000002177 Cataract Diseases 0.000 description 1
- 108091006146 Channels Proteins 0.000 description 1
- 108700010070 Codon Usage Proteins 0.000 description 1
- 206010056370 Congestive cardiomyopathy Diseases 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 102000004420 Creatine Kinase Human genes 0.000 description 1
- 108010042126 Creatine kinase Proteins 0.000 description 1
- OHOQEZWSNFNUSY-UHFFFAOYSA-N Cy3-bifunctional dye zwitterion Chemical compound O=C1CCC(=O)N1OC(=O)CCCCCN1C2=CC=C(S(O)(=O)=O)C=C2C(C)(C)C1=CC=CC(C(C1=CC(=CC=C11)S([O-])(=O)=O)(C)C)=[N+]1CCCCCC(=O)ON1C(=O)CCC1=O OHOQEZWSNFNUSY-UHFFFAOYSA-N 0.000 description 1
- 102100033149 Cytochrome b5 reductase 4 Human genes 0.000 description 1
- 102000000634 Cytochrome c oxidase subunit IV Human genes 0.000 description 1
- 108030005700 Cytochrome-b5 reductases Proteins 0.000 description 1
- 208000002155 Cytochrome-c Oxidase Deficiency Diseases 0.000 description 1
- 108090000365 Cytochrome-c oxidases Proteins 0.000 description 1
- 102100039868 Cytoplasmic aconitate hydratase Human genes 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 201000010046 Dilated cardiomyopathy Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108010089760 Electron Transport Complex I Proteins 0.000 description 1
- 102000008013 Electron Transport Complex I Human genes 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 208000036119 Frailty Diseases 0.000 description 1
- 102000003869 Frataxin Human genes 0.000 description 1
- 108090000217 Frataxin Proteins 0.000 description 1
- 230000010558 Gene Alterations Effects 0.000 description 1
- 206010018341 Gliosis Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 102100033039 Glutathione peroxidase 1 Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 101710178376 Heat shock 70 kDa protein Proteins 0.000 description 1
- 101000700892 Homo sapiens ATP synthase protein 8 Proteins 0.000 description 1
- 101000803294 Homo sapiens BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 Proteins 0.000 description 1
- 101150098499 III gene Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 102000005298 Iron-Sulfur Proteins Human genes 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 206010025421 Macule Diseases 0.000 description 1
- 102000013460 Malate Dehydrogenase Human genes 0.000 description 1
- 108010026217 Malate Dehydrogenase Proteins 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 208000036626 Mental retardation Diseases 0.000 description 1
- 201000002169 Mitochondrial myopathy Diseases 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 208000021642 Muscular disease Diseases 0.000 description 1
- 201000009623 Myopathy Diseases 0.000 description 1
- 102000000818 NADP Transhydrogenases Human genes 0.000 description 1
- 108010001609 NADP Transhydrogenases Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108020004485 Nonsense Codon Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 101710129178 Outer plastidial membrane protein porin Proteins 0.000 description 1
- 102100031538 Phosphatidylcholine-sterol acyltransferase Human genes 0.000 description 1
- 102000001105 Phosphofructokinases Human genes 0.000 description 1
- 108010069341 Phosphofructokinases Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 244000028344 Primula vulgaris Species 0.000 description 1
- 235000016311 Primula vulgaris Nutrition 0.000 description 1
- 102000013009 Pyruvate Kinase Human genes 0.000 description 1
- 108020005115 Pyruvate Kinase Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 102000002278 Ribosomal Proteins Human genes 0.000 description 1
- 108010000605 Ribosomal Proteins Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 108060007963 Surf-1 Proteins 0.000 description 1
- 101150041750 Surf4 gene Proteins 0.000 description 1
- 102100028532 Synaptophysin-like protein 1 Human genes 0.000 description 1
- 101710169338 Synaptophysin-like protein 1 Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 241000589596 Thermus Species 0.000 description 1
- 102100034769 Thioredoxin-dependent peroxide reductase, mitochondrial Human genes 0.000 description 1
- 101710150060 Thioredoxin-dependent peroxide reductase, mitochondrial Proteins 0.000 description 1
- 102100031274 Translocator protein Human genes 0.000 description 1
- 101710166801 Translocator protein Proteins 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 102100037820 Voltage-dependent anion-selective channel protein 1 Human genes 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- FMYKJLXRRQTBOR-BZSNNMDCSA-N acetylleucyl-leucyl-norleucinal Chemical compound CCCC[C@@H](C=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(C)=O FMYKJLXRRQTBOR-BZSNNMDCSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000007950 acidosis Effects 0.000 description 1
- 208000026545 acidosis disease Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000002424 anti-apoptotic effect Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 210000004227 basal ganglia Anatomy 0.000 description 1
- 108700000711 bcl-X Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229940049706 benzodiazepine Drugs 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 230000006931 brain damage Effects 0.000 description 1
- 231100000874 brain damage Toxicity 0.000 description 1
- 208000029028 brain injury Diseases 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 230000004094 calcium homeostasis Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000000692 cap cell Anatomy 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 230000001364 causal effect Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000004098 cellular respiration Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 238000005094 computer simulation Methods 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 230000001054 cortical effect Effects 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 208000026615 cytochrome-c oxidase deficiency disease Diseases 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000027721 electron transport chain Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 108091008053 gene clusters Proteins 0.000 description 1
- 102000054766 genetic haplotypes Human genes 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000007387 gliosis Effects 0.000 description 1
- 108010086596 glutathione peroxidase GPX1 Proteins 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 230000004116 glycogenolysis Effects 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 210000005003 heart tissue Anatomy 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 208000023692 inborn mitochondrial myopathy Diseases 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 238000011813 knockout mouse model Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229940049920 malate Drugs 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 230000007625 mitochondrial abnormality Effects 0.000 description 1
- 230000004065 mitochondrial dysfunction Effects 0.000 description 1
- 210000001700 mitochondrial membrane Anatomy 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000007659 motor function Effects 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 208000013435 necrotic lesion Diseases 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000000626 neurodegenerative effect Effects 0.000 description 1
- 230000007171 neuropathology Effects 0.000 description 1
- 230000037434 nonsense mutation Effects 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 206010029864 nystagmus Diseases 0.000 description 1
- 108010007425 oligomycin sensitivity conferring protein Proteins 0.000 description 1
- 210000003733 optic disk Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000008289 pathophysiological mechanism Effects 0.000 description 1
- 238000003909 pattern recognition Methods 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 108091008695 photoreceptors Proteins 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 230000000861 pro-apoptotic effect Effects 0.000 description 1
- 230000000770 proinflammatory effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 230000007055 protein processing involved in protein targeting to mitochondrion Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011155 quantitative monitoring Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000012950 reanalysis Methods 0.000 description 1
- 230000025915 regulation of apoptotic process Effects 0.000 description 1
- 230000022983 regulation of cell cycle Effects 0.000 description 1
- 108091006091 regulatory enzymes Proteins 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 210000003583 retinal pigment epithelium Anatomy 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012772 sequence design Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000005315 stained glass Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 210000001103 thalamus Anatomy 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 238000011830 transgenic mouse model Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 238000002759 z-score normalization Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31632301P | 2001-08-30 | 2001-08-30 | |
| CA002356540A CA2356540A1 (en) | 2001-08-30 | 2001-08-31 | Expressed dna sequences involved in mitochondrial functions |
| PCT/US2002/027886 WO2003020220A2 (en) | 2001-08-30 | 2002-08-30 | Mitochondrial biology expression arrays |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005515757A true JP2005515757A (ja) | 2005-06-02 |
| JP2005515757A5 JP2005515757A5 (enExample) | 2006-01-26 |
Family
ID=25682709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003524534A Pending JP2005515757A (ja) | 2001-08-30 | 2002-08-30 | ミトコンドリア生物学発現アレイ |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP1432823A4 (enExample) |
| JP (1) | JP2005515757A (enExample) |
| WO (1) | WO2003020220A2 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2270159B1 (en) | 2003-05-21 | 2015-01-07 | Andes Biotechnologies S.A. | Markers for pre-cancer and cancer cells and the method to interfere with cell proliferation therein |
| EP1633885A2 (en) * | 2003-06-09 | 2006-03-15 | Decode Genetics EHF. | Methods for predicting drug efficacy in patients afflicted with hypertension |
| EP1524523A1 (en) * | 2003-10-17 | 2005-04-20 | Deutsches Krebsforschungszentrum Stiftung Des Öffentlichen Rechts | Use of ADAM 12 for diagnosis and therapy of preeclampsia |
| WO2005058142A2 (en) * | 2003-12-16 | 2005-06-30 | Emory University | Diabetes diagnostic |
| CN114990160B (zh) * | 2022-06-13 | 2024-02-02 | 昆明医科大学 | 一种Navβ2-ICD低表达转基因鼠模型的构建方法及其应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69829402T2 (de) * | 1997-10-31 | 2006-04-13 | Affymetrix, Inc. (a Delaware Corp.), Santa Clara | Expressionsprofile in adulten und fötalen organen |
| CA2329709A1 (en) * | 1998-04-24 | 1999-11-04 | Mitokor | Compounds and methods for treating mitochondria-associated diseases |
| US6218117B1 (en) * | 1998-06-15 | 2001-04-17 | Mitokor | Compositions and methods for identifying agents that quantitatively alter detectable extramitochondrial DNA:mitochondrial DNA ratios |
| JP2003524397A (ja) * | 1999-03-16 | 2003-08-19 | マイトコー | オルガネラ遺伝子産物の示差的発現 |
-
2002
- 2002-08-30 WO PCT/US2002/027886 patent/WO2003020220A2/en not_active Ceased
- 2002-08-30 EP EP02768777A patent/EP1432823A4/en not_active Withdrawn
- 2002-08-30 JP JP2003524534A patent/JP2005515757A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1432823A2 (en) | 2004-06-30 |
| EP1432823A4 (en) | 2005-03-02 |
| WO2003020220A2 (en) | 2003-03-13 |
| WO2003020220A3 (en) | 2003-12-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20060099578A1 (en) | Mitochondrial biology expression arrays | |
| KR20100016525A (ko) | 녹내장 진행 리스크의 판정 방법 | |
| US6537751B1 (en) | Biallelic markers for use in constructing a high density disequilibrium map of the human genome | |
| Mandal et al. | Sequencing arrays for screening multiple genes associated with early-onset human retinal degenerations on a high-throughput platform | |
| KR20110094041A (ko) | 정상 안압 녹내장 질환 감수성 유전자 및 그 이용 | |
| AU2009275988A1 (en) | A genetic marker test for Brachyspina and fertility in cattle | |
| US20040203034A1 (en) | Optimization of cancer treatment with irinotecan | |
| TW200923103A (en) | ALPK1 gene variants in diagnosis risk of gout | |
| CA2324866A1 (en) | Biallelic markers for use in constructing a high density disequilibrium map of the human genome | |
| JP2005515757A (ja) | ミトコンドリア生物学発現アレイ | |
| JP2004535776A (ja) | ラット毒性関連遺伝子及びその使用 | |
| US20060234221A1 (en) | Biallelic markers of d-amino acid oxidase and uses thereof | |
| JP2005515757A5 (enExample) | ||
| EP1829979B1 (en) | Method of identifying gene with variable expression | |
| TW201311908A (zh) | 診斷犬之青光眼的方法及套組 | |
| WO2012173809A2 (en) | Method of identifying de novo copy number variants (cnv) using mz twins discordant for attention problems/disorders | |
| Bracken et al. | Genomewide association studies | |
| EP2707497B1 (en) | Detecting the brachyspina mutation | |
| AU2002331789A1 (en) | Mitochondrial biology expression arrays | |
| JP2004504037A (ja) | 肥満関連の二対立遺伝子マーカー地図 | |
| Alsobrook II | The genetics of Tourette syndrome | |
| MEHDI et al. | Biotechnology: The Discovery of Disease causing | |
| Hameed | MS Muhammad Ismail a Aiysha Abid 7 Institute of Biomedical And Genetic Engineeri.@ Sindh Institute of Urology and Transplantation | |
| Chiang | Comparative and integrative genomic approach toward disease gene identification: Application to Bardet-Biedl syndrome | |
| Buckley | Development and Application of Microarray-Based Comparative Genomic Hybridization: Analysis of Neurofibromatosis Type-2, Schwannomatosis and Related Tumors |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20050829 |
|
| A524 | Written submission of copy of amendment under article 19 pct |
Free format text: JAPANESE INTERMEDIATE CODE: A524 Effective date: 20051201 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20080704 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20081128 |