JP2005501557A - ｓｉＲＮＡノックアウトアッセイ法およびコンストラクト - Google Patents

ｓｉＲＮＡノックアウトアッセイ法およびコンストラクト Download PDF

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JP2005501557A
JP2005501557A JP2003525634A JP2003525634A JP2005501557A JP 2005501557 A JP2005501557 A JP 2005501557A JP 2003525634 A JP2003525634 A JP 2003525634A JP 2003525634 A JP2003525634 A JP 2003525634A JP 2005501557 A JP2005501557 A JP 2005501557A
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sequence
rna
polynucleotide
vector
complementary
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JP2005501557A5 (enExample
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ヘルト−ヤン・アルツ
エレン・フェラ・ランヘメイヤー
イフォ・ピースト
ヘルムート・ヘンドリクス・ヘラルドゥス・ファン・エス
ホーデフリドゥス・アウグスティヌス・マリア・ミヒエルス
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Galapagos Genomics NV
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    • C—CHEMISTRY; METALLURGY
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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
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    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays
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    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/022—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from an adenovirus

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  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
JP2003525634A 2001-09-01 2002-08-29 ｓｉＲＮＡノックアウトアッセイ法およびコンストラクト Pending JP2005501557A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31722901P 2001-09-01 2001-09-01
US38573302P 2002-06-04 2002-06-04
PCT/EP2002/009670 WO2003020931A2 (en) 2001-09-01 2002-08-29 Sirna knockout assay method and constructs

Publications (2)

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JP2005501557A true JP2005501557A (ja) 2005-01-20
JP2005501557A5 JP2005501557A5 (enExample) 2006-01-05

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US (2) US20030198627A1 (enExample)
EP (1) EP1444346B2 (enExample)
JP (1) JP2005501557A (enExample)
AT (1) ATE389016T2 (enExample)
AU (1) AU2002339458A1 (enExample)
CA (1) CA2457419A1 (enExample)
DE (1) DE60225586T3 (enExample)
WO (1) WO2003020931A2 (enExample)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005527198A (ja) * 2002-02-14 2005-09-15 シティ・オブ・ホープ 哺乳動物細胞において、干渉ｒｎａ分子を産生する方法、および該干渉ｒｎａ分子の療法的使用
JP2005533484A (ja) * 2002-01-22 2005-11-10 コールド スプリング ハーバー ラボラトリー Ｒｎａ干渉のための方法及び組成物
JP2008086201A (ja) * 2006-09-29 2008-04-17 Gifu Prefecture Kenkyu Kaihatsu Zaidan マイクロｒｎａ生成の検出方法と癌の診断・治療およびマイクロｒｎａ生成調整剤
JP2009528840A (ja) * 2006-03-07 2009-08-13 ザ・トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルバニア ランダムＲＮＡｉライブラリ、その生成方法、及びそれを使用したスクリーニング方法
JP2011200125A (ja) * 2010-03-24 2011-10-13 National Institute Of Advanced Industrial Science & Technology 改変された致死遺伝子及び該遺伝子を含むベクター

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JP2003526367A (ja) 2000-03-16 2003-09-09 ジェネティカ インコーポレイテッド Ｒｎａ干渉の方法とｒｎａ干渉組成物
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