JP2005239701A - Phonation-improving agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a phonation-improving agent having a phonation disturbance-preventing effect and/or phonation disturbance-improving effect, especially the phonation-improving agent capable of being utilized effectively as a food and an orally or transnasally used medicine or a quasi drug. <P>SOLUTION: This phonation-improving agent is a solvent extract of at least 1 kind of plant selected from the group consisting of Glycyrrhiza uralensis, elder, chamomile, lotus, carrot, Chinese matrimony vine, persimmon, hops, ginkgo, reddish kidney beans, white kidney beans, Phaseolus lathyroides, red kidney beans, chick pea, mottled kidney bean, bamboo grass, guava, Phaseolus radiatus, olive, comfrey, sassafras and peach, or by blending a solvent extract of black soybeans with the above as an active ingredient. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a specific plant-derived voicing enhancer that can be widely applied to foods, pharmaceuticals or quasi drugs used orally or nasally.

  The act of “speaking” expresses information, will, emotions, etc., and not only communicates with others, but also can be a barometer of his own health by listening to his / her own voice. If smooth utterance is hindered, it not only causes inconveniences in daily life, but also acts psychologically and causes stress. Therefore, it is considered important to enable “speech”, particularly “smooth utterance”, from the viewpoint of improving the quality of life (QOL).

  On the other hand, the efficacy of pharmaceuticals and functional foods in the existing pharyngeal region is aimed at the treatment and prevention of various symptoms mainly during colds such as antibacterial, anti-inflammatory, antitussive and expectorant of the throat, The reality is that it has not been fully considered from the viewpoint of making it easier to speak (improving speech), that is, QOL.

Black bean broth is traditionally known to have good effects on the throat and voice, as traditionally used as a folk medicine for the purpose of suppressing voice drowning and coughing (for example, (Refer nonpatent literature 1 etc.). For this reason, foods obtained by pulverizing an extract extracted from black beans as it is by a conventional method such as liquid or spray drying and blending it as a food material have been proposed as foods having voice squeezing suppression and antitussive effects.
Clinical and research, 75, No.12, 2625 (1998)

  An object of the present invention is to provide a voice improvement agent whose main component is a solvent extract derived from a natural product (plant) with high safety and whose main component is improvement in QOL. Another object of the present invention is to provide a method for preparing such a voice enhancement agent and a product that is taken or taken orally or nasally, such as a food containing the voice enhancement agent.

  The inventors of the present invention have been diligently studying day and night to achieve the above object. As a result, they have found that the solvent extract of a specific natural product (plant) contains an utterance-enhancing component. completed.

That is, this invention is an utterance improvement agent using the solvent extract of a specific plant hung up to the following items 1-3.
Item 1. Daylily, elder, chamomile, lotus, carrot, wolfberry, hop, ginkgo, golden bean, white beans, purple flower beans, red peas, chickpeas, quail beans, bearfish, guava, bundo beans, olives, comfrey, sassafras And at least one plant solvent extract selected from the group consisting of peaches and peaches.
Item 2. Black beans, licorice, elder, chamomile, lotus, carrot, wolfberry, persimmon, hop, ginkgo, golden bean, white kidney beans, purple flower beans, red peas, chickpeas, quail beans, cubs, guava, bundo beans, olives, comfrey , Sassafras, and a peach improvement agent characterized by containing the solvent extract of two or more plants selected from the group which consists of peaches.
Item 3. Item 3. The voice enhancement agent according to Item 1 or 2, wherein the solvent is water, a polar organic solvent, or a mixture thereof.

Furthermore, this invention is a product which uses the said speech-improving agent listed as the following item 4-5 as an active ingredient.
Item 4. Item 4. A food containing the voice enhancement agent according to any one of Items 1 to 3, or a drug or quasi-drug used orally or nasally.
Item 5. Item 5. The food according to item 4, wherein the food is a beverage, confectionery, or supplement, or a pharmaceutical or quasi-drug used orally or nasally.

  Hereinafter, the present invention will be described in more detail.

  The present invention is a voice improvement agent using a natural product (plant) solvent extract.

  The “speech enhancer” as used in the present invention is an agent that has an effect of maintaining a normal state (smooth utterance) or a utterance in a singing voice that is used routinely in conversation or singing. It means what has the effect of quickly returning from an abnormal state to a normal state. In addition, “maintaining utterance in a normal state” means, in other words, “an effect of preventing a normal utterance to an abnormal utterance (voicing disorder)” (also referred to as “a voice disorder prevention effect” in the present invention). In other words, “the effect of promptly returning the utterance from the abnormal state to the normal state” means “the effect of improving the abnormal utterance (uttering disorder) to the normal utterance” (in the present invention, “the utterance disorder improving effect”). ")". Preferably, it has both a “voice disturbance prevention effect” and a “voice disturbance improvement effect”.

  As a method of evaluating whether an utterance is abnormal or normal, for example, a voice obtained by continuously uttering a vowel (/ a /) with a strength of a certain time f is digitally recorded, and the voice quality ( A method for aural evaluation of (voice level) (auditory evaluation), a method for acoustic analysis of the voice quality (voice level) by sound spectroscopy, and a method for evaluating from the subject's awareness of hoarseness (subjective evaluation) Can be mentioned. Among them, acoustic analysis and auditory evaluation by sound spectrometry are methods for objectively evaluating the distinction between utterance abnormalities and normality, and subjective evaluation is a method for subjectively evaluating the distinction between utterance abnormalities and normality. Any of these can be used effectively. The determination can be made by any one of these methods, but it is preferable to make a comprehensive determination by combining two or more methods.

  The “speech enhancer” targeted by the present invention exhibits the above effects when taken or taken orally or nasally.

Plants used as extraction objects in the present invention include licorice (spanish licorice (scientific name Glycyrriza glabra L.), licorice (scientific name Glycyrrhiza urarensis Fisch.) And other leguminous licorice genus), elder (honeysuckle family elderberry genus) Sambucus nigra L.), chamomile (Roman chamomile (scientific name: Anthemis nobilis L.), German chamomile (scientific name: Matricaria chamomilla L.), etc., lotus ( Nelumbo nucifera Gaertn), carrot [Ginseng (scientific name Panax ginseng CAMeyer), Sancychin ginseng (scientific name Panax notoginseng ), American carrot (scientific name Panax quinquefolium L.), etc., wolfberry ( Lycium chinense Mill.) persimmon (Diospyros Department of the genus Diospyros Scientific name Diospyros kaki Thunb.), hop (mulberry family Surumusu genus Name Humulus lupulus L.), ginkgo (Ginkgo Department of ginkgo species scientific name Ginkgo biloba L.), striped bamboo (Poaceae Sasa genus Scientific name Sasa veitchii Rehd.), Guava (Myrtaceae Banjirou species scientific name Psidium guajava L.), olive (Oleaceae Olive genus scientific name Olea europea L.), Comfrey (Purpleaceae genus Symphytum officinale L.), Sassafras ( Sassafras albidum Nutt.), And peach ( Prunus persica (L.) Plants such as Batsch); and gold bean (seed (genus Phaseolus vulgaris L. seed)), white beans (seed ( Phaseolus vulgaris L. seed)), purple bean (seed) leguminous green beans genus Scientific name Phaseolus coccineus L. seeds (grain)), red peas (legumes pea species scientific name Pisum sativum L. seeds (grain)), chick peas (legumes chickpeas Scientific name Cicer arietinum L. seeds (grain)), quail beans (seeds (grain of leguminous green beans genus Scientific name Phaseolus vulgaris L.)), Bundou beans (legume Vigna Scientific name Vigna radiata L. seeds (grain )) And black beans (seed of the leguminous soybean genus Glycine max (L.) Merrill). These can be used alone or in combination of two or more.

  Among the above, licorice, elder, chamomile, lotus, carrot, wolfberry, cocoon, hop, ginkgo, kumaba, guava, olive, comfrey, sassafras, and peach are not particularly limited, and the entire plant body It may be a part of a plant body such as a root, stem, branch, leaf, flower, fruit or seed. Suitable extraction sites include lotus, camellia, ginkgo, bear moth, guava, olive, sassafras, and peach leaves; wolfberry leaves and fruits; comfrey branches and leaves; elder and chamomile flowers; Roots and rhizomes for carrots; for carrots the roots or roots are steamed and dried;

  As a solvent used for extraction, water, a polar organic solvent, or a mixture thereof can be exemplified. Examples of the polar organic solvent include alcohol, acetone, ethyl acetate and the like. Among these, from the viewpoint of safety to the human body and handleability, water, lower alcohol, or a mixture thereof can be mentioned. Examples of lower alcohols include C1-C4 lower alcohols such as methanol, ethanol, propanol, isopropyl alcohol, and butanol. Preferred is water or hydrous alcohol (particularly hydrous ethanol). In addition, when using hydrous alcohol (especially hydrous ethanol) as a solvent, it is preferable that the amount of alcohol (ethanol) contained in it is 40 volume% or less.

  The extraction solvent may be adjusted to be acidic. The adjustment to acidity can be performed using an inorganic acid such as hydrochloric acid, sulfuric acid or phosphoric acid, or an organic acid such as citric acid or malic acid so that the pH is in the range of about 2 to 5.5.

  As the extraction method, a generally used method can be employed. Although not limited, for example, a method of immersing the above-mentioned various plants (as-is, or crude powder, shredded product) in a solvent under low temperature, warming or boiling conditions; low temperature, warming or boiling conditions Examples of the extraction method include stirring under stirring; a supercritical extraction method using carbon dioxide gas; or a percolation method. Preferably, it is a method of immersing the various plants that have been subjected to the drying treatment (as-is, or coarse powder, shredded products) in a solvent under high temperature or boiling conditions, and extracting them while stirring as necessary (heat treatment) Extraction method).

  The obtained extract can remove solids (residues) unnecessary for the solvent by various solid-liquid separation means such as filtration, coprecipitation, centrifugation, and pressing as required. The obtained extract can be used as it is by adding it to food or the like, but can be used after appropriately diluted with a food solvent such as water or ethanol. Furthermore, it can be used as an extract in the form of powder or paste by freeze-drying or concentration. Moreover, the commercially available extract formulation prepared in this way can also be used as it is or diluted and concentrated.

  In addition, when using black beans as a plant, although not limited, from the viewpoint of removing undesirable odors, tastes and / or colors, the extract obtained above is further treated with enzymes or / and It is preferable to carry out any combination of fractionation treatments having a molecular weight of 5000 to 25000, and use what is thus obtained.

  Here, examples of the enzyme used for the enzyme treatment include an enzyme having an action of degrading a sugar or / and a protein. Specifically, for example, as the former saccharide-degrading enzyme, amylase such as Christase Y (manufactured by Yamato Kasei), Sumiteam S (manufactured by Shin Nippon Chemical Industry), cellulase A (manufactured by Amano Enzyme), and cellulosin AC40 (HIV eye) Enzymes having a saccharide-degrading activity such as cellulase such as Sucrase S (manufactured by Sankyo) and hemicellulase such as Sumiteam X (manufactured by Shin Nippon Chemical Industry Co., Ltd.) can be used. Examples of the latter proteolytic enzymes include acidase proteases such as Newase A (manufactured by Amano Enzyme) and Protease YP-SS (manufactured by Yakult Yakuhin Kogyo); Sumiteam FP (manufactured by Shin Nippon Chemical Industry), and Entilon NBS (洛) Neutral proteases such as Tokasei Kogyo Co .; enzymes having proteolytic activity such as alkaline proteases such as Novozymes FM (Novozymes) and Biolase SP-4FG (Nagase ChemteX) can be used.

  Such an enzyme having a saccharide-degrading activity and an enzyme having a proteolytic activity may be used singly or in combination. The enzyme to be used may be a single enzyme, but various enzymes may be used in combination. A preferred enzyme is an enzyme having a saccharide-degrading activity, and in particular, an enzyme having an activity of degrading a polysaccharide such as the above-mentioned cellulase, amylase, hemicellulase or the like can be suitably used.

  The enzyme treatment can be carried out under predetermined conditions suitable for each enzyme used. The temperature condition for performing the enzyme treatment is not particularly limited, but a range of 30 to 80 ° C. can be usually used. Preferably it is 35-60 degreeC.

  Examples of the fractionation treatment having a molecular weight of 5000 to 25000 include a gel filtration method, a membrane separation method, and an adsorption treatment method. A membrane separation method is preferred. The membrane separation method used here is not particularly limited as long as a fraction having a molecular weight of 5000 to 25000 can be obtained as a result from the water-soluble extract of black beans. Specifically, the high molecular weight is set using a membrane having a set molecular weight cut off, for example, in the range of about 20,000 to 30,000, preferably in the range of about 22,000 to 28,000, more preferably around 25,000. A processing method for separating and removing molecular compounds, and a set molecular weight cutoff is, for example, in the range of about 1,000 to 8,000, preferably in the range of about 3,000 to 7,000, more preferably around 5,000. It is preferable to carry out in combination with a treatment method for separating and removing low molecular weight compounds using a membrane.

  As a method of separating and removing the former polymer compound, specifically, NTU-3150 membrane, NTU-3250 membrane, NTU-3550 membrane, NTU-3800 UF membrane (Nitto Denko) ); Cefilt-UF (manufactured by NGK); AHP-2013 membrane, AHP-3013 membrane, AHP-1010 membrane (above, manufactured by Asahi Kasei); . As a method for separating and removing the latter low molecular weight compounds, specifically, NTR-7250 membrane, NTR-7410 membrane, NTR-7430 membrane, NTR-7450 membrane (Nitto Denko) ), AIP-3013 membrane, ACP-3013 membrane, ACP-2013 membrane, AIP-2013 membrane, AIO-1010 membrane (above, manufactured by Asahi Kasei) and other reverse osmosis membrane (nanofiltration membrane) treatment Or ultrafiltration (UF) membrane treatment.

  Examples of the membrane material used for the membrane separation method include cellulose, cellulose diacetate or triacetate, polyamide, polysulfone, polystyrene, polyimide, polyacrylonitrile and the like, regardless of whether they are natural, synthetic, or semi-synthetic. . In addition, as a membrane used for the fractionation treatment, a microfiltration (MF) membrane or the like can be used in addition to the ultrafiltration membrane and the reverse osmosis membrane.

  The enzyme treatment and the molecular weight fractionation treatment can be performed in any combination continuously or discontinuously with the black bean extract or the treated product. The order of the combination is not particularly limited, and any of the order of molecular weight fractionation treatment following the enzyme treatment and the order of molecular weight fractionation treatment followed by the enzyme treatment may be used, but the former method is preferred. In addition to the above enzyme treatment and fractionation treatment with a molecular weight of 5,000 to 25,000, the black bean extract or its treatment product is further subjected to normal purification such as acid treatment, extraction treatment, adsorption treatment, ion exchange treatment or membrane treatment. It is also possible to perform the processing used in These treatments can be performed on a black bean extract or a treated product thereof, either alone or in combination of two or more.

  In the solvent extracts of the various plants thus obtained, as shown in the experimental examples to be described later, components having a voice disturbance prevention effect and / or a voice disturbance improvement action (hereinafter these components are collectively referred to as “voice production”). It is also referred to as “enhancing action component”. Therefore, the said extract can be used conveniently as an active ingredient of a voice improvement agent and a voice improvement agent. The extract can be used as it is or after being concentrated and used as a voice enhancement agent or an active ingredient thereof. In addition, the extract can be used as a solid according to a conventional method such as concentration to dryness or spray drying. It can also be used as a voice enhancer or an active ingredient thereof in a state dispersed or dissolved in ethanol, ethanol or a mixture thereof.

  The extract thus obtained is in a solution state dissolved or dispersed in water, alcohol (eg, ethanol) or other solvent, or a solid state (powder, granule, tablet, Various preparations (food [eg, supplements], pharmaceuticals or quasi-drugs) that are taken or ingested orally or nasally. Examples of foods having such a formulation include supplements of various shapes, rice cakes (such as throat lozenges), troches, and drinks; and examples of pharmaceuticals or quasi drugs include various oral products such as mouthwashes, Examples include gargles, troches, drinks, propellants, various products for throat such as inhalants or inhalants, and nasal drops.

  In addition to various plant solvent extracts, such preparations may contain food sanitation or pharmaceutically acceptable carriers and additives depending on the product form. Specific examples of such carriers and additives include, for example, food additives such as dextrin, lactose, powdered starch syrup, preservatives, stabilizers, fragrances, sweeteners or antioxidants commonly used in these products or pharmaceuticals. Can be listed as acceptable additives.

  Therefore, the present invention also includes foods (including functional foods and supplements) that have an effect of improving voice production by including the solvent extract of the plant of the present invention prepared by the above method, as well as orally or nasally. It provides quasi-drugs and pharmaceuticals to be administered.

  Such foods include: (1) milk beverages, lactic acid bacteria beverages, fruit juice soft drinks, soft drinks, carbonated drinks, fruit juice drinks, vegetable drinks, vegetable / fruit drinks, alcoholic drinks, powdered drinks, coffee drinks, tea drinks, tea drinks (2) Puddings such as custard pudding, milk pudding, souffle pudding, pudding with fruit juice, desserts such as jelly, bavaroa and yogurt; (3) Ice cream, ice milk, lacto ice, milk ice cream, fruit juice Ice cream and soft ice cream, ice candy, sherbet, frozen desserts such as ice confectionery; (4) gums such as chewing gum and bubble gum (plate gum, sugar-coated grain gum); (5) In addition to coated chocolate such as marble chocolate Flavors such as strawberry chocolate, blueberry chocolate and melon chocolate Chocolate such as chocolate; (6) Hard candy (including bonbon, butterball, marble, etc.), soft candy (including caramel, nougat, gummy candy, marshmallow, etc.), drop, toffee, etc .; (7) Baked confectionery such as hard biscuits, cookies, rice crackers, rice crackers; (The above (2) to (7) are collectively referred to as confectionery); fruit wine such as red wine, liqueur, chuhai, carbonated alcoholic beverages, etc. Can be mentioned. Preferably, beverages such as the soft drinks and confectionery are used. Among them, (4) gums, (5) chocolates, (6) strawberries, and (7) baked confectionery can be suitably exemplified as confectionery.

  As a ratio of various plant extracts to be blended in these foods, when prepared as a beverage such as a soft drink, 0.001 to 20% by weight, preferably 0.1 to 5% in terms of the dry matter weight of the various plant extracts % By weight: 0.1% to 10% by weight in terms of dry weight of various plant extracts when prepared as a gum; in terms of dry weight of various plant extracts when prepared as baked goods such as cookies 0.1 to 50% by weight, preferably 0.5 to 5% by weight; in the case of preparation as candy such as candy, 0.01 to 30% by weight, preferably 0.1 to 10% by weight, in terms of dry matter weight of various plant extracts % Can be exemplified.

  In addition, as quasi-drugs and pharmaceuticals, various oral products such as mouthwash as described above, throat products such as mouthwashes, troches, drinks, propellants, inhalants or inhalants, and nasal drops are included. Can be mentioned. Examples of the proportion of various plant extracts to be blended in these products include 0.001 to 80% by weight, preferably 0.1 to 50% by weight, in terms of the dry matter weight of the various plant extracts.

  The voicing enhancer of the present invention or the product of the present invention (food, medicine or quasi-drug) containing the same as an active ingredient has a good voicing enhancing action (voicing disorder preventing action and / or voicing disorder improving action). The subject can be given a smooth utterance. In particular, since the voice enhancement agent of the present invention uses highly safe plant components derived from nature as an active ingredient, it is effective not only as a pharmaceutical that exhibits a voice improvement effect, but also as a food that is consumed daily or its ingredient. Can be used.

  Examples, comparative examples, and experimental examples are described below in order to clarify the configuration and effects of the present invention. However, the present invention is not affected by these examples.

Example 1 Licorice extract A commercially available licorice extract preparation (manufactured by Nippon Powder Chemical Co., Ltd .: a viscous liquid obtained by hot water extraction of licorice roots) was used.

Example 2 Elder extract 30.2 g of dried elder flowers were placed in 400 ml of water, immersed, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 8.7 g of extract powder.

Example 3 Chamomile extract German chamomile 20.0 g of dried flowers was placed in 400 ml of water, and after immersion, extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 6.5 g of extract powder.

Example 4 Lotus leaf extract 20.0 g of dried lotus leaf was placed in 400 ml of water, immersed, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 3.6 g of extract powder.

Example 5 Ginseng (carrot) extract A commercially available ginseng preparation (manufactured by Nippon Powder Chemical Co., Ltd .: a viscous liquid obtained by extracting roots of ginseng with 30% aqueous ethanol) was used.

Example 6 Extract of wolfberry leaf 10.2 g of dried wolfberry leaves was placed in 250 ml of water, immersed, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 3.5 g of extract powder.

Example 7 wolfberry fruit extract A commercially available wolfberry fruit extract preparation (manufactured by Nippon Powder Chemical Co., Ltd .: a viscous liquid obtained by extracting wolfberry fruit with 25% aqueous ethanol) was used.

Example 8 10.3 g of dried leaves of koji leaf extract koji were placed in 250 ml of water, immersed, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 2.3 g of extract powder.

Example 9 Hop Extract A commercially available hop extract preparation (manufactured by Nippon Powder Chemical Co., Ltd .: a viscous liquid obtained by extracting hop bulbs with 40% aqueous ethanol) was used.

Example 10 Ginkgo biloba extract 10.3 g of dried ginkgo biloba was put in 250 ml of water, immersed, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 3.0 g of extract powder.

Example 11 Kintoki Bean Extract 50.5 g of dried Kintome Bean was placed in 350 ml of water, immersed in the refrigerator overnight, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 6.5 g of extract powder.

Example 12 White kidney extract 6.1 g of extract powder was prepared from 50.5 g of dried white beans by the same extraction method as in Example 11.

Example 13 Purple Flower Bean Extract 7.4 g of extract powder was prepared from 50.6 g of dried purple flower beans by the same extraction method as in Example 11.

Example 14 Red Pea Extract 5.2 g of extract powder was prepared from 51.2 g of dried red pea by the same extraction method as in Example 11.

Example 15 Chick Bean Extract 5.3 g of extract powder was prepared from 49.9 g of dried chickpeas by the same extraction method as in Example 11.

Example 16 Quail Bean Extract A 6.3 g extract powder was prepared from 52.1 g of dried quail beans by the same extraction method as in Example 11.

Example 17 Kumagusu extract 12.2 g of dried Kumagusu leaves were placed in 250 ml of water, and after immersion, extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 1.9 g of extract powder.

Example 18 Guava extract 10.1 g of dried guava leaves was placed in 250 ml of water, immersed, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 2.2 g of extract powder.

Example 19 bundo bean extract 3.2 g of extract powder was prepared from 50.0 g of dried bundo beans by the same extraction method as in Example 11.

Example 20 Olive leaf extract 10.9 g of dried olive leaf was placed in 250 ml of water, immersed, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 2.6 g of extract powder.

Example 21 Comfrey extract 10.2 g of comfrey dried leaf with branches was placed in 250 ml of water, immersed, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 2.6 g of extract powder.

Example 22 Sassafras extract 10.0 g of dried sassafras leaves were placed in 250 ml of water, immersed, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 1.9 g of extract powder.

Example 23 Peach leaf extract 10.1 g of dried peach leaves were placed in 250 ml of water, immersed, and then extracted by boiling for 2 hours. After cooling, the filtrate obtained by suction filtration was freeze-dried to prepare 3.2 g of extract powder.

Example 24 10 kg of leguminous plant (black beans) seeds were added to 70 L of black bean extract water and left overnight at room temperature, followed by boiling for 2 hours. The hot water extract thus obtained was subjected to solid-liquid separation, and the obtained extract was mixed with a filter aid and diatomaceous earth and subjected to suction filtration to obtain about 55 L (pH 5.8) of a filtrate. 10 g of enzyme / cellulase (Cellulosin AC40 (manufactured by HIBI), titer: 4,000 units / g) was added to 55 L (pH 5.8) of the hot water extraction filtrate, and the mixture was slowly stirred at 40-55 ° C. for enzyme treatment. A liquid was obtained. Next, the enzyme-treated solution was concentrated under reduced pressure to obtain 22 kg of concentrated extract (solid content: 70%). After adding 70 g of water to 10 g of this concentrated extract, 7 g of black bean extract preparation was prepared by lyophilization.

Comparative Example 1 Chrysanthemum Extract Extract powder of chrysanthemum extract (comparative) by operating in the same manner as in Example 23 except that chrysanthemums (dried flowers 10.1 g) were used instead of the peach leaves used in Example 23. Article 1) was prepared.

Experimental Example 1 Evaluation of the effect of preventing vocal dysfunction Using the solvent extracts of various plants prepared in Examples 1 to 23, the preventive effect against voice disorders (voice disorders) (ie, the effect of maintaining the voice in a normal state) evaluated. The voicing disorder preventive effect of the solvent extracts (Examples 1 to 23) of the various plants is the chrysanthemum flower extract (Comparative product 1) prepared in Comparative Example 1, the Karin extract formulation (manufactured by Oshiro Pharmaceutical Co., Ltd., Comparative Product 2), and As a control, evaluation was performed by comparing with the effect of preventing dysphonia of sugar (Comparative Product 3).

<Experimental procedure>
The following tests were conducted on 3 healthy adult males (25 to 55 years old) who were aware of a voice disorder when performing daily continuous speech. After taking any of 210 mg of various plant solvent extracts (invention products: Examples 1 to 23) as a solid content into the subject's oral cavity, f (forte) The voice disturbance was caused by reading continuously for a long time.

  Before and after the start of reading, digitally record a voice that continuously utters the vowel a (/ a /) with an intensity of f for about 3 seconds, and perform sound analysis of the sound quality (voice level) by sound spectroscopy went.

  In addition, for the three healthy adult males (25 to 55 years old), 210 mg (as solids) of a different type of plant solvent extract from the sample taken above was prepared in Comparative Example 1. Chrysanthemum flower extract (Comparative product 1) 210 mg, 210 mg of Karin extract preparation (Comparative product 2) as a solid content, and 210 mg of sugar (Comparative product 3) as a control were also ingested in the same way, read books, and impaired voice After inducing, the degree of dysphonia was evaluated.

<Result>
The acoustic analysis of the recorded sound by sound spectrumography is from CSA4400 computer made by KAY Elemetrics, from the amplification of the voice signal uttered to the capture to the computer, the recording of digital data (voice waveform), and the sound spectrumography analysis. A speech lab was used.

  Sound spectroscopy is a method for analyzing the power spectrum of a voice. Not only voice but also time-series data such as sound and waveform data are formed by overlapping sine waves of various frequencies having various amplitudes and phases, that is, partial sounds. Although the phase of partial sound is important when discussing waveforms and sound source localization, voice quality, phonological properties, etc. depend mainly on the relationship between frequency and amplitude (more precisely, power). Since many voices change from moment to moment, the partial sounds that make up the voice also change. In order to express this time change, the voice is decomposed into the frequency and power of partial sound by Fourier transform (FFT), and the horizontal axis represents time and the vertical axis represents frequency, and the partial sound power is expressed in intensity. Is sound spectroscopy. The graph analyzed by sound spectrography is called a sound spectrogram (refer to “2nd edition, Voice Examination Clinical Edition”, Spoken Language Medicine, 1999, page 164).

  FIGS. 1 to 7 show sound spectrumgrams of speech before and after reading (inducing voice disturbance) in the case where each subject ingests each sample, taking the same subject as an example. In addition, FIG. 1 is the result (FIGS. AD) of the solvent extracts (inventions) of various plants prepared in Examples 1 to 4, and FIG. 2 is the solvent extract of various plants prepared in Examples 5 to 8 (inventions). (Invention product) results (Figs. E to H), Fig. 3 shows the results of solvent extracts (invention products) of various plants prepared in Examples 9 to 12 (Figs. I to L), and Fig. 4 shows Examples 13 to 16 FIG. 5 shows the results of the solvent extracts (inventions) of the various plants prepared in Example 1 (FIGS. MP) and FIG. 5 shows the results of the solvent extracts (inventions) of the various plants prepared in Examples 17-20 (FIGS. Q to T). ), FIG. 6 shows the results (FIGS. U to W) of the solvent extracts (inventions) of various plants prepared in Examples 21 to 23, and FIG. 7 shows the chrysanthemum flower extract (Comparative product 1) (FIG. X) and Karin. It is a result of an extract formulation (comparative product 2) (FIG. Y) and sugar (comparative product 3) (FIG. Z). In either case, the sound spectrumgram of the sound before the start of reading (left in each figure) shows that the fundamental frequency and harmonics are less fluctuating, the fluctuation width is almost constant, and the sound is normally uttered. Observed.

  As can be seen from the figure, when the chrysanthemum extract (Comparative product 1), Karin extract preparation (Comparative product 2), and 210 mg of sugar (Control, Comparative product 3) have been read (after voice disturbance) The sound spectrum gram (right in each figure) has a large fluctuation range of the fundamental frequency, the horizontal stripes of harmonic components at high frequencies are very unclear and cannot be confirmed (see Figures X, Y, and Z), and there is a lot of glottal noise in the voice It was shown to be included. On the other hand, in the case of ingesting the solvent extracts of the various plants of Examples 1 to 23 (product of the present invention), the fluctuations of the fundamental frequency and the harmonic component that were the same as before reading were detected as clear horizontal stripes. (See FIGS. A to W), it was revealed by sound spectrum analysis that normal speech was maintained even after reading was finished (after voice failure was induced).

From the above, licorice, elder, chamomile, lotus, carrot (grape carrot ) , wolfberry, cocoon, hops, ginkgo, gold beans, white beans, purple flower beans, red peas, chickpeas, quail beans, bear candy, guava, bundo It was confirmed that the bean, olive, comfrey, sassafras, and peach extracts have an excellent preventive effect against voice disorders (an effect of maintaining vocalization in a normal state).

Experimental Example 2 Evaluation of the vocalization enhancing action when combining two types of plant extracts By combining two types of solvent extracts of various plants prepared in Examples 1 to 24, one type of solvent extract of various plants is used. It was evaluated whether the improvement effect (ie, the effect of improving from abnormal utterance (voicing disorder) to normal utterance) on the voice disturbance (voice dysfunction) is enhanced as compared with the case where the voice utterance was present.

<Experimental procedure>
The following tests were conducted on 3 healthy adult males (25 to 55 years old) who were aware of a voice disorder when performing daily continuous speech. First, voice disturbance was caused by allowing the subject to read the book f (forte) freely selected for a long period of time. Next, 52.5 mg of various plant solvent extracts (invention products: Examples 1 to 24) as solids were combined (105 mg in total) (FIG. 8) and freely taken into the oral cavity of the subject. Whether or not the degree of the disorder was improved was judged by a four-level evaluation (1: unchanged, 2: slightly improved, 3: improved, 4: considerably improved) (voice impairment improvement effect: sensory evaluation). In addition, as a comparative control, the sensory evaluation of the voice disturbance improvement effect was similarly performed when the solvent extract (Examples 1 to 24) (105 mg) consisting of one kind of various plants was ingested instead of the sample.

<Result>
The results are shown in FIG. The table shows the average score of sensory evaluation (four levels) regarding the speech disturbance improvement effect. As can be seen from the above, the solvent extracts of various plants (Examples 1 to 24) can be combined or combined to produce a higher dysphonic disorder improvement effect (speech improvement effect) when combined with two or more kinds of soy intake. It was shown that

  The voice-enhancing agent of the present invention has a naturally-occurring ingredient having experience of eating as an active ingredient, and thus exerts an action to improve voice (or to prevent dysphonia or / and to improve dysphonia) by oral or nasal administration and ingestion. It can be used effectively as a medicine or food (including supplements).

Solvent extracts of various plants prepared in Examples 1 to 4 [A: licorice extract (Example 1), B: elder extract (Example 2), C: chamomile extract (Example 3), and D : It is a sound spectrumgram which shows the speech disorder prevention effect of a lotus leaf extract (Example 4). The left figure is the sound spectrumgram before the start of reading, and the right figure is the sound spectrumgram at the end of reading after ingesting each solvent extract (after inducing voice disturbance) (hereinafter the same in FIGS. 2 to 9). ). Solvent extracts of various plants prepared in Examples 5 to 8 (E: Panax ginseng extract (Example 5), F: wolfberry leaf extract (Example 6), G: wolfberry fruit extract (Example 7), And H are sound spectrumgrams showing the effect of preventing dysphonia of the koji leaf extract (Example 8). Solvent extracts of various plants prepared in Examples 9 to 12 (I: Hop extract (Example 9), J: Ginkgo biloba extract (Example 10), K: Kintoki bean extract (Example 11) , And L are sound spectrumgrams showing the dysphonia prevention effect of white kidney extract (Example 12)). Solvent extracts of various plants prepared in Examples 13 to 16 (M: purple flower bean extract (Example 13), N: red pea extract (Example 14), O: chickpea extract (Example 15) , And P: Sound spectrumgram showing the dysphonia prevention effect of quail bean extract (Example 16)). Solvent extracts of various plants prepared in Examples 17-20 (Q: Kumagusu extract (Example 17), R: Guava extract (Example 18), S: Bundu bean extract (Example 19), and T: a sound spectrumgram showing the effect of preventing dysphonia of the olive leaf extract (Example 20). Solvent extracts (U: Comfrey extract (Example 21), V: Sassafras extract (Example 22), and W: Peach leaf extract (Example 23) prepared in Examples 21 to 23 It is a sound spectrumgram which shows a speech disorder prevention effect. For the Kikuhana extract (Comparative product 1) (Fig. X), Karin extract preparation (Comparative product 2) (Fig. Y), and Sugar (Comparative product 3) (Fig. Z) It is a result. It is a figure which shows the result of having evaluated the utterance improvement effect at the time of combining two types of plant extracts in Experimental example 2. FIG.

Claims (5)

Licorice, elder, chamomile, lotus, carrot, wolfberry, hop, ginkgo, golden bean, white kidney beans, purple flower beans, red peas, chickpeas, quail beans, bearfish, guava, bundo beans, olives, comfrey, sassafras And at least one plant solvent extract selected from the group consisting of peaches and peaches. Black beans, licorice, elder, chamomile, lotus, carrot, wolfberry, persimmon, hop, ginkgo, golden bean, white kidney beans, purple flower beans, red peas, chickpeas, quail beans, cubs, guava, bundo beans, olives, comfrey , Sassafras, and a peach improvement agent characterized by containing the solvent extract of two or more plants selected from the group which consists of peaches. The voicing enhancer according to claim 1 or 2, wherein the solvent is water, a polar organic solvent or a mixture thereof. A food containing the voice enhancement agent according to any one of claims 1 to 3, or a pharmaceutical or quasi-drug used orally or nasally. The food according to claim 4, or a pharmaceutical or quasi-drug used orally or nasally, wherein the food is a beverage, confectionery, or supplement.

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