KR20110069297A - A composition for removing hangover comprising an extract of mango - Google Patents

Abstract

PURPOSE: A composition containing mango extract for relieving hangover is provided to reduce blood alcohol concentration and to promote in vivo alcohol metabolism. CONSTITUTION: A composition for relieving hangover contains mango extract as an active ingredient. The mango is an apple mango. The mango extract is obtained by freeze-drying a mango, preparing the mango in a coarse powder form, and treating with ethanol at room temperature for 3 days. A pharmaceutical composition for relieving hangover contains the composition containing mango extract as an active ingredient. The mango extract is administered in 10-3000mg/kg/day. The pharmaceutical composition is used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, and suppository.

Description

Hangover composition containing mango extract {A composition for removing hangover comprising an extract of mango}

The present invention relates to a hangover relief composition, and more particularly to a hangover relief composition containing a mango extract as an active ingredient.

Alcohol consumed by human body is metabolized about 30% by alcohol dehydrogenase (ADH) and microsomal ethanol oxidizing system (MEOS) of gastrointestinal mucosa.

Ethanol metabolism in the liver is mainly accomplished by NAD-linked enzymes, alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). These enzymes produce acetaldehyde and acetate, respectively, and acetate is converted to acetyl-CoA to generate energy through the TCA cycle or to synthesize cholesterol and fatty acids. Both ADH and MEOS in the stomach and liver metabolize alcohol to oxidize acetaldehyde in the first step, so that the amount of acetaldehyde produced increases as alcohol is metabolized by these enzymes.

Acetaldehyde binds to vital active substances, including proteins, in vivo, causing symptoms such as increased pulse, sweating, nausea and vomiting. In the second stage of alcohol metabolism, acetaldehyde dehydrogenase (ALDH) oxidizes acetaldehyde into acetate to prevent the accumulation of acetaldehyde and its side effects, but more than 15% of Koreans have inactive form of ALDH. The above symptoms may result from the accumulation of acetaldehyde when drinking.

A hangover refers to headache, diarrhea, anorexia, nausea, vomiting, chills and cold sweating after drinking alcohol. Objective symptoms can be defined as perception, decreased exercise ability, hematologic changes, and hormonal changes.

Alcohol is known to have various effects on liver metabolism depending on the amount of intake. Acetaldehyde and NADH produced during the oxidation process damage liver cells rather than alcohol itself.

Alcohol absorbed into the body reacts with NAD ions by ADH (alchol dehydrogenase) to convert NADPH and acetaldehyde, and then reacts with NAD ions to convert acetaldehyde into NADPH and acetate by ALDH (aldehyde dehydrogenase). The hangover is caused by acetaldehyde and requires both ADH and ALDH before the hangover can be resolved.

In Korea's drinking culture, many people are showing high interest in drugs or drinks that can remove hangovers due to heavy drinking and frequent drinking, and the development of hangover drink and health food is increasing.

The present inventors have diligently researched to relieve hangovers such as headache, heartburn, and vomiting. As a result, the composition containing mango extract as an active ingredient reduces the alcohol content in the blood after drinking, alcohol dehydrogenase and acetaldehyde. It was confirmed that the dehydrogenase activity can be increased, and the present invention has been completed.

Therefore, an object of the present invention is to provide a composition that can effectively remove the hangover without side effects on the human body.

According to one aspect of the present invention, the present invention provides a hangover relief composition containing the mango extract as an active ingredient.

Mango is a fruit belonging to the family Sumac, and its origin is Southeast Asia. It is mainly distributed in subtropical regions such as Myanmar, northern India, and Mexico. Mango is one of the representative tropical fruits along with bananas, pineapples, papayas and avocados (FAO, 2007).

The mango of the present invention includes an apple mango.

Mangoes of the present invention include mature fruits.

The present inventors conducted the experiment by dividing the mango into the skin part and the flesh part in order to investigate the effect of the mango extract on hangover.

Extraction method of the mango maturation and rind extract, mango maturation and pulp extract of the present invention may be used a common extraction method known in the art, it is also extracted with a hydrophilic organic solvent, such as water or alcohol, or a mixed solvent thereof Can be. Preferably extracted with ethanol.

In the present invention, the mango extract is prepared in the form of coarse powder using a freeze-dried apple mango, and then treated with ethanol can be extracted for 3 days at room temperature.

The ethanol is preferably treated with 1L of 80% ethanol per 10g dry weight.

In one embodiment of the present invention, the crude mango crude powder was filtered from the extracted mixture, and the filtrate was concentrated under reduced pressure and dried, and the dried concentrate was used to dissolve 500 µg / g mouse weight.

In one embodiment of the present invention in order to determine the effect of the mango crude extract on the alcohol content change commercially available hangover drink (life 808 ™) as a positive control, apple mango mature and flesh (Mango) in the experimental group Flesh), Apple Mango Peel (Mango Peel) extracts were orally administered to mice and 50% alcohol was orally administered one hour later. The mango pulp extract of the present invention significantly reduced blood alcohol content compared to the PBS control (Example 3).

In addition, in one embodiment of the present invention, mango rind extract and mango pulp extract were found to increase the activity of alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH) in the body after ingestion of alcohol (Example 4).

In addition, it was confirmed that the apple mango mature peel extract caused a lot of changes in the metabolic profiling of blood after alcohol intake (Example 5).

In the case of the mango pulp extract (MF) of the present invention, the blood alcohol concentration is significantly reduced, but the activity of alcohol degrading enzymes such as ADL and ALDH has no significant effect compared to the mango rind extract (MP), Metabolic profiling did not show a significant change, so rather than absorbing mango pulp extract, it seems that mango pulp extract has a role in inhibiting the gastrointestinal absorption of alcohol itself. On the other hand, in the case of mango rind extract, the decrease in blood alcohol concentration was lower than that of mango rind extract, but it was found to increase the enzymatic activity of ADL and ALDH and to affect the metabolic profiling in the blood. It is thought to play a role in promoting. Apple mango mature flesh and rind are also lower than other women's commercial hangover-removing beverages, which lowers blood alcohol concentrations, while mango rind extract directly decomposes acetaldehyde that causes hangovers. The enzyme activity of ALDH, an enzyme, was increased even more than that of dawn, indicating that pulp and rind of apple mango maturity had a hangover effect.

Mango extract having a hangover relief function of the present invention may further contain one or more active ingredients exhibiting the same or similar functions.

Mango extract having a hangover-relieving function of the present invention contains minerals such as vitamins B, C, E, beta-carotene, Ca, Mg, Zn, phospholipids such as lecithin or maltol, oligosaccharides, amino acids, etc. Compounds of the present invention can be used, and among them, the use of 2 to 3 components in vitamin C, E or beta-carotene, maltol, etc. is more preferable because it can enhance the biological activity effect.

In addition to the above components, in order to enhance the taste as a known additive, natural flavors such as plum, lemon, pineapple or herb, or natural fruit juice, natural pigments such as chlorophyllin and flavonoid, and fructose, sweet sugar Can be mixed with honey, sugar alcohol, sugar and acidulants such as citric acid and sodium citrate.

Another aspect of the present invention provides a pharmaceutical composition for hangover relief comprising the mango extract as an active ingredient.

The mango extract may be a mango rind extract or mango pulp extract, or may be an extract containing both the rind and the pulp.

The composition may be formulated into a pharmaceutical unit dosage form by adding a pharmaceutically acceptable carrier, excipient or diluent to the mango rind extract.

The composition of the pharmaceutical unit dosage form of the present invention is in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, oral dosage forms, sterile injectable solutions, suppositories, and transdermal formulations according to conventional methods. Can be formulated and used. Carriers, excipients and diluents that may be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, Microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. If necessary, it is formulated with diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, surfactants and the like.

In one embodiment, the composition containing the mango rind extract of the present invention may be formulated as a solid preparation for oral administration. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules and the like, which include at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin and the like. It is formulated by mixing. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used.

In another embodiment, the composition containing the mango rind extract of the present invention may be formulated into a liquid preparation for oral administration. Liquid preparations for oral administration include suspensions, solvents, emulsions, syrups, and the like. In addition to the inert diluents commonly used (e.g., purified water, ethanol, liquid paraffin), various excipients may be used, for example. Wetting agents, sweetening agents, fragrances, preservatives and the like can be included.

In another embodiment, the composition containing the mango rind extract of the present invention may be formulated into a formulation for parenteral, preferably intraperitoneal administration.

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As a sterile aqueous solution, suitable buffer solutions such as Hanks' solution, Ringer's solution, or physically buffered saline can be used.For non-aqueous solvents and suspensions, propylene glycol, polyethylene glycol, olive oil, Vegetable oils, injectable esters such as ethyloleate, and the like can be used. If necessary, preservatives, stabilizers, wetting or emulsifying agents, salts for controlling osmotic pressure and / or buffers may also be used. On the other hand, suppositories, such as witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin, and the like may be used.

Compositions formulated in such a manner can be administered in various amounts, including parenteral or oral (dermal, subcutaneous, intravenous, intramuscular, intraperitoneal, etc.) in an effective amount. As used herein, an "effective amount" refers to an amount exhibiting a prophylactic or therapeutic effect when administered to a patient. The dosage of the composition according to the present invention may be appropriately selected depending on the route of administration, subject of administration, age, sex, weight, individual difference and disease state. Preferably, the composition of the present invention can vary the content of the active ingredient according to the degree of disease, preferably 1 to 10,000 mg / kg body weight / day, more preferably 10 to 3000 mg / kg body weight / effective amount Can be administered.

As another embodiment of the present invention, there is provided a dietary supplement showing a hangover effect comprising the mango extract as an active ingredient.

The health food containing the mango extract functioning to relieve hangover of the present invention includes a folk remedy for the hangover, such as tea, jelly, juice, extract, drink, etc., wherein the mango extract is an active ingredient. . The health food of the present invention processed in various forms as described above is excellent in detoxification without side effects to the human body, easy to take and can be stored for a long period of time can be usefully used to relieve hangovers before and after drinking.

Mango extract functioning to relieve hangover of the present invention can be added to health food for the purpose of relieving hangover. When the mango extract of the present invention is used as a food additive, the mango extract may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, in the preparation of food or beverage, the mango extract of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on 100 parts by weight of the raw material. However, in the case of long-term intake for health and hygiene or health control purposes, the amount may be below the above range, and there is no problem in terms of safety, so the active ingredient may be used in an amount above the above range.

The mango extract of the present invention can be usefully used as a hangover remedy without side effects by promoting alcohol metabolism in vivo by reducing the alcohol content in the blood and increasing the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase.

Hereinafter, the examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples according to the gist of the present invention, and modifications, substitutions, and insertions commonly known by those skilled in the art It will be apparent to those skilled in the art that the present invention may be included in the scope of the present invention.

Example 1 Extraction of Experimental Materials and Samples

Apple mango was lyophilized by dividing the mature fruit into skin and flesh parts. Freeze-dried apple mango was prepared in the form of coarse powder using a mortar, and then treated with 1L of 80% ethanol per 10g of dry weight and extracted for 3 days at room temperature. Apple mango crude powder was filtered from the mixture, and the filtrate was concentrated under reduced pressure and dried. The dried concentrate was used in animal experiments by dissolving 150 μL in PBS to 500 μg / g mouse weight.

Example 2 Animal Experiment

Animal experiments were carried out with 8 weeks old male ICR mice. The experiment was divided into four groups and the control group was administered with PBS instead of the sample. As a positive control, a commercial hangover drink (dawn 808 ™) was used. Apple mango maturation and pulp (MF) and apple mango maturation and rind (MP) were used as experimental groups.

Water was removed the day before the experiment and feed was removed in the morning of the experiment day. Samples used in the experimental group were dissolved in PBS to 500μg per g of the mouse, and then 150μL orally administered, PBS and Dawn orally 150μL each. After 1 hour, 150 μL of 50% alcohol was orally administered. One hour after alcohol administration, blood was drawn from the heart. The collected blood was centrifuged at 13,000 rpm and 4 ° C. for 30 minutes to obtain plasma.

Example 3 Measurement of Blood Alcohol Content

Blood alcohol content was used with alcohol (ethanol) kit (r-biopharm, Germany).

A blood sample was measured at 340 nm for 3 minutes in NAD-dissolved calcium diphosphate buffer (measured value after 3 minutes, a1), and then the ADH solution was added for 3 minutes at 340 nm (3 Measured value in minutes, a2)

ΔA = (a2-a1)

Blood ethanol content (%) = ΔA (sample) / ΔA (control) × 100

The group receiving the fruit of the mature fruit had a 50% or more reduction in alcohol content than the PBS control group. The 100% blood alcohol content of the PBS-administered group was 75.97 ± 25.01% for the age group (YM), 41.28 ± 7.99% for the mature flesh (MF), and the mature skin (MP). ) Is 68.95 ± 7.89% (Table 1, FIG. 2).

TABLE 1 Blood Alcohol Content (Mean ± SEM) (n = 4)

Processed sample Blood alcohol content (%) PBS 100 ± 12.33 Dawning (YM) 75.97 ± 25.01 Mature Pulp (MF) 41.28 ± 7.99 Mature rind (MP) 68.95 ± 7.89

Example 4 ADH, Cytosol ALDH Enzyme Activity

4-1. Preparation of Alcohol Degrading Enzyme Sources (ADH, ALDH) in Liver Tissue

Liver was extracted from mice used in the experiment, washed with 0.25 M sucrose (2 mM mercaptoethanol and 10 mM sodium phosphate pH 7.4) and homogenized by adding 7-fold solution of sucrose solution. The homogenized for 10 min centrifugation at aekreul 600 × g, and again centrifuged for 10 minutes at 10,000 × g and the supernatant was the supernatant as the cytoplasmic ALDH (cytoplasmic acetaldehyde dehydrogenase) measuring an enzyme source, the upper layer of the at 10,000 × g The solution was centrifuged again at 105,000 × g for 1 hour, and the supernatant was used as an enzyme source of ADH (alcohol dehydrogenase).

4-2. How to measure the activity of ADH (in vitro)

The activity of ADH was measured by modifying the method of Blandino (Bostia, et al., 1978), and the rate of NADH production at an absorbance of 340 nm was used as an index. The reaction solution was prepared by adding 100 μL of ethanol to 0.1 M Tris-HCl buffer containing 5 mM NAD (pH 8.8), and then adding 100 μL of ADH enzyme source. Measured for minutes.

4-3. Methods for measuring cytosol ALDH activity (in vitro)

100 μL of cytoplasm after adding 4-methylpyrazole and acetaldehyde to a final concentration of 0.1 M in 60 mM sodium pyrophosphate buffer (pH 8.8) containing 4 mM NAD The amount of NADH produced by adding ALDH enzyme source was measured at 340 nm for 5 minutes.

Percent enzyme activity was calculated as follows.

ΔA = (stop absorbance-starting absorbance)

Enzyme activity (%) = ΔA (sample) / ΔA (control) × 100

4-4. ADH, cytoplasmic ALDH enzyme activity

As a result of comparing the effects of apple mango crude extract on ADH activity, ADH activity in the daylight- and sample-administered group was higher than that in the PBS-administered control group. The highest activity of DH (YM) was 144.34 ± 1.45%, and ADH activities of mature flesh (MF) and mature skin (MP) treatment groups were 119.29 ± 3.95% and 129.76 ± 7.49%, respectively. 2, FIG. 3).

TABLE 2 ADH enzyme activity (mean ± SEM) (n = 4)

Processed sample ADH activity (%) PBS 100 ± 2.66 Dawning (YM) 144.34 ± 1.45 Mature Pulp (MF) 119.29 ± 3.95 Mature rind (MP) 129.76 ± 7.48

The activity of cytoplasmic ALDH was best with the enzyme activity of 137.73 ± 6.78% in the group treated with mature skin (Table 3, Figure 4).

TABLE 3 ALDH enzyme activity (mean ± SEM) (n = 4)

Processed sample Cytoplasmic ALDH Activity (%) PBS 100 ± 3.20 Dawning (YM) 81.00 ± 3.70 Mature Pulp (MF) 114.83 ± 7.33 Mature rind (MP) 137.73 ± 6.78

Example 5 PCA, PLS-DA Results Analysis

5-1. ^One H-NMR analysis

Metabolic profiling of apple mango mature pulp and rind was used to define the characteristics of each site, and ¹ H-NMR analysis was performed to compare and analyze plasma sample differences between control and apple mango extract fed groups.

^{For 1} H-NMR analysis, 0.1 g of lyophilized apple mango powder was placed in a test tube for centrifuge, and 5 ml of 80% ethanol was added for 1 minute, followed by vortexing and sonication, respectively, at 2,000 rpm. Centrifugation for 20 minutes. The extract was transferred to a 50 ml round flask and concentrated in a vacuum condenser and used for NMR analysis.

Concentrated apple mango was dissolved in 1 ml of D ₂ O, filtered and placed in a 5-mm NMR tube. KH ₂ PO ₄ was placed in D ₂ O as a buffer and the pH of D ₂ O used for NMR was adjusted to 6.0 using 520 μl of 1 N NaOD.

All Apple mango spectra were measured at 298 K temperature using an Avance 600 spectrometer (Bruker Analytische GmbH, Rheinstetten, Germany) at Bruker 600.13-MHz. Spectra were recorded using zgcppr as the presaturation pulse sequence for water molecule inhibition. The relaxation delay was 1 second, with 128 scans collected at 64 K data points. The spectrum was 11363.6 Hz wide and a acquisition time of 3.04 seconds per scan number was used. Prior to the Fourier transformation (FT), a typical line extension of 0.3-Hz was applied to the free induction decay.

On the other hand, frozen blood plasma sample is dissolved in a water bath at 40 ℃, D ₂ O 3- (trimethyl-silil (0.05% of the plasma samples in 0.3 ml 0.2 ml) - propionamide -2,2,3,3- d 4 acid, sodium Salt containing; TSP was pipetted into a 5-mm NMR tube (Norell, NJ) with D ₂ O as an internal standard) and shaken for several seconds to mix thoroughly. KH ₂ PO ₄ was placed in D ₂ O as a buffer and the pH of D ₂ O used for NMR was adjusted to 6.0 using 520 μl of 1 N NaOD.

All plasma spectra were measured using a NMR spectrometer (Avance 600 FT-NMR, Bruker, Germany) at a temperature of 298 K with a triple-axis inverse (TXI) cold probe at a quantum NMR frequency of 600.13 MHz. Spectra were recorded using zgpr as the presaturation pulse sequence for water molecule inhibition. 128 scans were recorded for each sample with the following parameters: 0.155 Hz / point, pulse width 4.0 μs (30 °), relaxation delay 2 seconds.

5-2. Data Processing

Apple mango spectra were partitioned into spectral regions ranging from δ = 0.52 to 10.00, 0.04 ppm wide using AMIX program (version 3.7, Biospin, Bruker) to make a total of 237 regions per NMR spectrum. The range of δ = 4.6 to 4.9 was excluded from the analysis because it is an indication of water residual in the aqueous extract. Spectral data were converted to Microsoft Excel format and SIMCA-P software (version 12.0, Umetrics, Umea, Sweden) was used for multivariate statistical analysis. In this analysis, all the spectral data were centered on the mean without scaling.

In the plasma spectrum, the range of δ = 4.8 to 5.0 was excluded from the analysis because it is an indication of the residual water in the aqueous extract, and the peaks of the alcohol injected into the experimental animals ranged from δ = 1.16 to 1.24 and δ = 3.64 to 3.72. . Everything else is the same as the Apple Mango data processing above.

5-3. Multivariate Statistical Analysis

Spectral data of 80% ethanol extracts from apple mango maturation were applied by principal component analysis to distinguish apple mango maturity and mature skin. Spectral data on plasma samples were subjected to principal component analysis and partial least squares-discriminant analysis to feed metabolites in plasma after feeding PBS control, apple mango mature flesh and mature skin, respectively. We saw a change in profiling.

5-4. PCA, PLS-DA Results

Metabolic profiling of apple mango mature pulp and rind was observed to be divided on the PCA score plot (FIG. 5). This indicates that the apple mango mature pulp and the skin's metabolic profiling differ markedly.

The effect on the metabolic profiling in the blood of these mature pulp and rinds can be observed on the PCA score plot (FIG. 6). The alcohol component in the metabolic profiling was removed from the spectrum, so the results of this PCA score plot (FIG. 6) show the effect of PBS, MP, and MF on metabolites in the blood except alcohol. In the case of feeding PBS and MP, it is divided by PC1. This is because ingestion of alcohol after feeding MP significantly changed the metabolic profiling of blood compared to the intake of alcohol only (PBS control). It can be seen that. On the other hand, MF is also dispersed among individuals, and does not seem to have a significant effect on the metabolic profiling after alcohol consumption compared to MP. The PLS-DA score plot shows this trend more clearly. Mouse plasma ingested alcohol after administration of PBS and MF on the basis of PLS component 1 is shown in the positive direction, but in the case of MP, it is different from the PBS control and MF. (FIG. 7).

Example 6 Relative Concentrations of Acetaldehyde and Acetate in Blood

As a result of comparing the relative concentrations of acetaldehyde in each group, only a small amount of acetaldehyde was produced in MF, and the relative concentration of acetate in blood was not significantly different in each group (FIGS. 8 and 9).

TABLE 4 Relative Concentrations of Acetaldehyde in Blood (Mean ± SEM) (n = 4)

Processed sample Acetaldehyde content in blood (%) PBS 100 ± 1.69 Mature Pulp (MF) 92.45 ± 2.39 Mature rind (MP) 95.48 ± 1.53

TABLE 5 Relative Concentrations of Acetate in Blood (Mean ± SEM) (n = 4)

Processed sample Blood Acetate Content (%) PBS 100 ± 6.09 Mature Pulp (MF) 93.72 ± 9.77 Mature rind (MP) 82.48 ± 7.65

1 shows alcohol degradation metabolism process.

Figure 2 shows the blood alcohol content in the PBS control group, dawn (YM) administration group, mature pulp administration group (MF), mature dermal administration group (MP) (mean ± SEM, n = 4, *: P <0.005).

Figure 3 shows the degree of ADH enzyme activity in the PBS control group, dawn (YM) administration group, mature pulp administration group (MF), mature skin administration group (MP) (mean ± SEM, n = 4, *: P <0.05, ** : P <0.01, ***: P <0.001).

Figure 4 shows the degree of ALDH enzyme activity in the PBS control group, dawn (YM) administration group, mature pulp administration group (MF), mature skin administration group (MP) (mean ± SEM, n = 4, ***: P <0.001)

Figure 5 shows the PCA score plot results of ¹ H-NMR spectral data for 80% ethanol extract of apple mango mature and pulp and skin.

FIG. 6 shows PCA score plots of ¹ H-NMR spectral data for apple mango maturation and mouse plasma fed flesh and skin.

Figure 7 shows the results of the PLS-DA score plot of the ¹ H-NMR spectral data for apple mango maturation and mouse plasma fed flesh and skin.

8 shows the relative concentration of acetaldehyde in blood.

9 shows the relative concentrations of acetate in blood.

Claims (7)

Hangover relief composition containing mango extract as an active ingredient. The composition of claim 1, wherein the mango is apple mango. The composition of claim 1, wherein the extract is prepared by preparing lyophilized mango in the form of coarse powder, and then extracting the same by treating ethanol. The composition according to any one of claims 1 to 3, wherein the extract is a mango rind extract or a mango pulp extract. Hangover pharmaceutical composition comprising a composition containing the mango extract of claim 1 as an active ingredient. The composition of claim 5, wherein the mango extract is contained in an amount of 10 to 3000 mg / kg body weight / day. Health supplement food exhibiting a hangover effect comprising the composition of claim 1 as an active ingredient.

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