CN101780225A - Method for extracting angiotensin I converting enzyme inhibitor from reed leaves - Google Patents
Method for extracting angiotensin I converting enzyme inhibitor from reed leaves Download PDFInfo
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- CN101780225A CN101780225A CN200910249030A CN200910249030A CN101780225A CN 101780225 A CN101780225 A CN 101780225A CN 200910249030 A CN200910249030 A CN 200910249030A CN 200910249030 A CN200910249030 A CN 200910249030A CN 101780225 A CN101780225 A CN 101780225A
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Abstract
The invention discloses a method for extracting angiotensin I converting enzyme inhibitor from reed leaves, which takes reeds as raw material, and products prepared by the method have the advantages of high security, low cost and industrialized production without side effects. Dry stems and leaves of reeds are placed into an extraction apparatus after being cut and milled, and a certain amount of ethanol solution or acetone solution are added to soak the stems and leaves, and the extracting solution is removed after heating and refluxing. A certain amount of water is used for heating and extracting, the extracting solution is collected and mixed after cooling, products are processed through vacuum concentration at 60 DEG C, and extracts from stems and leaves of reeds are obtained through freezing and drying. Tests on inhibition of angiotensin I converting enzyme activity demonstrate that the inhibitor has significant effect of inhibiting angiotensin I converting enzyme (ACE) activity.
Description
Technical field:
The present invention relates to a kind of method of ACEI inhibitor, especially a kind of is raw material with the phragmites communis, but the method for from Folium Phragmitis, extracting ACEI inhibitor that product has no side effect, the cheap industrialization of safe, cost is produced.
Background technology:
Hypertension is modal cardiovascular disease, is a principal element that threatens adult's health.In the human body, (Angiotensin I-converting enzyme ACE) is angiotonase-angiotensin system (Renin-angiotension system, RAS) most important adjusting hinge to angiotensin I-conversion enzyme.ACE can cut off the angiotensin I (Angiotensin I) from hepatic secretion and be converted into angiotensin II (Angiotensin II) on the one hand, and angiotensin II impels vasoconstriction, causes blood pressure; On the other hand, the kallikrein in the blood plasma cuts off kininogen albumen and produces bradykinin (Bradykinin), and bradykinin promotes vasodilation, makes blood pressure drops, yet ACE but makes bradykinin decompose the passivation bradykinin; All caused hypertension aspect ACE these two in human body.If suppressed the activity of ACE, just can suppress hypertensive generation.
Present antihypertensive drugs is divided into five big classes: disturb adrenergic nerve medicine, vasodilation medicine, calcium antagonist medicine, diuretic antihypertensive medicine and angiotensin-conerting enzyme (ACE) inhibitor.After wherein angiotensin-conerting enzyme (ACE) inhibitor acts on renin-angiotensin system, can effectively regulate, control human blood-pressure, be applied to the treatment of multiple cardiovascular disease, as congestive heart disease, myocardial infarction, diabetic nephropathy and renal dysfunction etc. are represented medicine such as captopril (captopril).Though captopril can stop the activity of ACE, the also side effect of various degrees is as cough, dyspepsia reaction, hepar damnification, postural hypotension, kidney injury, leukopenia etc.
Phragmites communis (Phragmi tes communis Trin.) is a grass family phragmites communis platymiscium, and the title of second forest is often arranged.In China, phragmites communis mainly is grown in areas such as the Three River Plain, northeast, the Huanghe delta, Xinjiang, Baiyang Lake, Hebei and the Yangtze river basin.Root and Ye Junke are used as medicine.Compendium of Material Medica and " Chinese medicine voluminous dictionary " record Folium Phragmitis nature and flavor are sweet, cold, nontoxic.Cure mainly vomiting and dirarrhea, haematemesis, maggot blood, pain in the lung, carbuncle on the back, cholera vomiting, lung heat clearing preventing or arresting vomiting etc., up to now also not about from phragmites communis, extracting the relevant report of ACEI inhibitor.
Summary of the invention:
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, providing a kind of is raw material with the phragmites communis, but the method for from Folium Phragmitis, extracting ACEI inhibitor that product has no side effect, the cheap industrialization of safe, cost is produced.
Technical solution of the present invention is a kind of method of extracting ACEI inhibitor from Folium Phragmitis, it is characterized in that carrying out as follows successively:
A. get exsiccant phragmites communis stem and leaf, put into extractor after shredding grinding, adding volume soaks than the alcohol-water or the acetone soln that are 10～30ml/g with the reed stem leaf quality, heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 1～3 time, and each extraction time is 1.0～3.0h, discard extracting solution, and will remain stem and leaf and dry and be extract;
B. add volume and carry out second extraction with extract quality than the water that is 5～30ml/g in extract, extract temperature and cause boiling temperature for reaching, extraction time is 1～3 time, and each extraction time is 1.0～3.0h;
C. collect the cooled decocting in water extracting solution of merging, at 60 ℃ of following concentrating under reduced pressure, lyophilization gets the reed stem leaf extract.
Alcohol is 70%～100% with the volume ratio of water in the alcohol-water solution of described a step, and wherein alcohol can be methanol, ethanol or propanol.
The present invention is to be raw material with wide material sources, cheap phragmites communis, utilize solvent extraction method that Folium Phragmitis is carried out boiling water extraction, resulting extract suppresses activity experiment through ACE to be proved, have the active effect of significant inhibition ACE and have no side effect, safe, cost is cheap, can be widely used in preparation and reduce in hypertensive medicine and the health product.The present invention is simple to operate, and solvent for use is general chemistry reagent, easily repeats, and is suitable for large-scale production.
The specific embodiment:
Embodiment 1:
A. accurately take by weighing exsiccant phragmites communis stem and leaf 10g, put into extractor after shredding grinding, ethanol (methanol or the propanol)-aqueous solution soaking that adds 100ml70%, adopt apparatus,Soxhlet's to extract 2 times, each 2 hours, extract temperature for reaching, discard extracting solution, will remain stem and leaf and dry and be extract to remove little polar substances such as pigment to boiling temperature;
B. in extract, add 100ml pure water decocting in water and extract, extract each 2h 2 times;
C. collect the cooled decocting in water extracting solution of merging, at 60 ℃ of following concentrating under reduced pressure, lyophilization gets the reed stem leaf extract.
Get the reed stem leaf extract of embodiment 1, the adding distil water standardize solution, compound concentration is the reed stem leaf extract solution of 5mg/mL, carry out ACE by existing method (coming quantitatively by HPLC as decomposing the hippuric acid that obtains with extract effect ACE enzyme and tripeptides) and suppress activity experiment, recording suppression ratio is 49%.
Embodiment 2:
A. accurately take by weighing exsiccant phragmites communis stem and leaf 5g, put into extractor after shredding grinding, add in the 50ml acetone soln and soak, adopt apparatus,Soxhlet's to extract 3 times, each 1 hour, extract temperature for reaching, discard extracting solution, will remain stem and leaf and dry and be extract to remove little polar substances such as pigment to boiling temperature;
B. in extract, add 50ml pure water decocting in water and extract, extract each 1h 3 times;
C. collect the cooled decocting in water extracting solution of merging, at 60 ℃ of following concentrating under reduced pressure, lyophilization gets the reed stem leaf extract.
Get the reed stem leaf extract of embodiment 2, adding distil water standardize solution, compound concentration are the reed stem leaf extract solution of 12.5mg/mL, carry out ACE by existing method and suppress activity experiment, and recording suppression ratio is 53%.
Embodiment 3:
A. accurately take by weighing exsiccant phragmites communis stem and leaf 10g, put into extractor after shredding grinding, add in the 100ml ethanol solution and soak, adopt apparatus,Soxhlet's to extract 1 time, each 1 hour, extract temperature for reaching, discard extracting solution, will remain stem and leaf and dry and be extract to remove little polar substances such as pigment to boiling temperature;
B. in extract, add 200ml pure water decocting in water and extract, extract each 3h 1 time;
C. collect cooled decocting in water extracting solution, at 60 ℃ of following concentrating under reduced pressure, lyophilization gets the reed stem leaf extract.
Get the reed stem leaf extract of embodiment 3, the adding distil water standardize solution, dilute successively that compound concentration is respectively 50,25,12.5, the Folium Phragmitis extract solution of 6.25mg/mL, carry out ACE by art methods and suppress activity experiment, record four extracts under the concentration ACE is all had the activity of inhibition, its activity exists certain dose-dependence, IC
50Value is 10.12mg/mL.
The extract that the embodiment of the invention 1～3 prepares can be used as the various preparations of treatment and prophylaxis of hypertension and related cardiovascular disease medicine thereof, the health food of inhibition hypertension and the food additive that conduct brings high blood pressure down etc.Medicine and health product are to mix with adjuvant to form various forms of pills, powder, tablet, unguentum, powder, injection, water preparation, granule, capsule, injection etc.; The health food form can be liquid such as the picture refreshment drink, soda pop, flavoring agent; Can join the wheaten food class as additive, fruit jelly, ice cream, curing food, Petaso, point is in the heart.
Claims (2)
1. method of extracting ACEI inhibitor from Folium Phragmitis is characterized in that carrying out as follows successively:
A. get exsiccant phragmites communis stem and leaf, put into extractor after shredding grinding, adding volume soaks than the alcohol-water or the acetone soln that are 10～30ml/g with the reed stem leaf quality, heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 1～3 time, and each extraction time is 1.0～3.0h, discard extracting solution, and will remain stem and leaf and dry and be extract;
B. add volume and carry out second extraction with extract quality than the water that is 5～30ml/g in extract, extract temperature and cause boiling temperature for reaching, extraction time is 1～3 time, and each extraction time is 1.0～3.0h;
C. collect the cooled decocting in water extracting solution of merging, at 60 ℃ of following concentrating under reduced pressure, lyophilization gets the reed stem leaf extract.
2. the method for from Folium Phragmitis, extracting ACEI inhibitor according to claim 1, it is characterized in that: the concentration of the alcohol-water solution of described a step is 70%～100%, wherein alcohol is methanol, ethanol or propanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910249030A CN101780225A (en) | 2009-12-30 | 2009-12-30 | Method for extracting angiotensin I converting enzyme inhibitor from reed leaves |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910249030A CN101780225A (en) | 2009-12-30 | 2009-12-30 | Method for extracting angiotensin I converting enzyme inhibitor from reed leaves |
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| CN101780225A true CN101780225A (en) | 2010-07-21 |
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| CN200910249030A Pending CN101780225A (en) | 2009-12-30 | 2009-12-30 | Method for extracting angiotensin I converting enzyme inhibitor from reed leaves |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999691A (en) * | 2010-11-12 | 2011-04-06 | 盐城工学院 | Reed leaf sausage and manufacturing method thereof |
| CN102132711A (en) * | 2010-12-29 | 2011-07-27 | 华宝食用香精香料（上海）有限公司 | Total flavonoid extracts of reed and preparation method thereof |
-
2009
- 2009-12-30 CN CN200910249030A patent/CN101780225A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 李瑞光等: "芦苇黄酮提取液体外抗氧化特性研究", 《西北农业学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999691A (en) * | 2010-11-12 | 2011-04-06 | 盐城工学院 | Reed leaf sausage and manufacturing method thereof |
| CN101999691B (en) * | 2010-11-12 | 2012-12-05 | 盐城工学院 | Reed leaf sausage and manufacturing method thereof |
| CN102132711A (en) * | 2010-12-29 | 2011-07-27 | 华宝食用香精香料（上海）有限公司 | Total flavonoid extracts of reed and preparation method thereof |
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Application publication date: 20100721 |