JP2005220328A - Method for producing additive containing indigo, indirubin or isatin - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To efficiently produce a useful additive by extending the usefulness of raw leaves and to decrease the generation of waste residue when the raw leaves are processed. <P>SOLUTION: A method for producing the additive containing indigo, indirubin or isatin comprises a step of freezing reaped raw leaves, a step of thawing the frozen raw leaves, a step of hydrolyzing indican contained in the leaves if necessary by adding β-glucosidase enzyme to an enzyme existing in the thawed raw leaves, a step of oxidizing indoxyl produced by hydrolyzing, and a step of making the raw leaves containing indigo, indirubin or isatin obtained by oxidizing into a muddy or a paste-like form by adjusting the water content of the raw leaves. If necessary, the additive of the muddy or the paste-like form is made into a powder by further removing the water through drying and by pulverizing it by using a crusher. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention mainly produces an additive mainly comprising at least one of indigo, indirubin or isatin from an indican-containing plant, for example, interior materials, building materials, dyes, medical-related products, quasi drugs, hair dye materials, The present invention relates to a method for producing an additive containing indigo, indirubin, or isatin that can be used as a food, health food, food, or the like.

  For example, since ancient times, indican plants such as Tadeai have been dyed on cloth etc. everywhere in the world, and they are also used for private use such as bactericidal effects, cuts and insect bites, medicinal products such as fever and detoxification, etc. It is said to have medicine, deodorizing effect, flame retardancy, and UV absorption, and has been useful. Although it is an indican plant with such excellent efficacy, with the transition of the times, a method for industrially producing useful substances has been developed, and indigo dyeing has been replaced by chemical artificial indigo and chemical dyes. It was faded. In recent years, however, there has been a movement toward reviewing useful substances obtained from natural materials due to the growing awareness of nature.

  Natural indigo dyeing is a dyeing that requires a high level of expertise and labor, and it is written in the literature that it cannot be easily done at home. The inventor of the present invention first developed a set capable of indigo dyeing for easily dyeing fabric even at home (Patent Document 1).

  As an example of a useful substance derived from fresh leaves, indigo obtained from Tadeai, which is an indican plant, is most known. Indigo is mainly used for dyeing, but other than that, as described above, it is known that it has excellent effects such as antiseptic, insecticidal, antibacterial, deodorizing, and ultraviolet absorption. Therefore, if a natural additive utilizing these properties can be produced industrially, the application is further expanded. For example, interior materials using additives, boards, wallpaper, and other building materials, not just dyeing indigo, but high-performance, high-quality building materials with antibacterial and insect-proofing effects. Can do. Further, by using natural materials, it is not necessary to use chemical substances that cause sick house syndrome, which has been a problem in recent years. In addition, blue, red, and green foods can be newly developed as foods to which the effects of useful substances are added. Furthermore, it can be applied to tree-free paper that does not use pulp. In this way, by using the additive for various purposes, the production and consumption of indican-containing plants can be expanded, leading to the activation of traditional industries that Japan is proud of.

  Indigo was mentioned as an example of a useful substance derived from fresh leaves, but in order to use it for dyeing, "sukumo" must be manufactured. After harvesting the plant, considerable time and labor are required until the fermentation process, and thereafter, the fermentation takes 100 days. In the meantime, since the amount of water to be applied and the temperature of fermentation must be controlled, the creation of “sukumo” requires a great deal of labor, time and technology. In addition, since it emits a unique odor during fermentation, there is also a problem that it is disgusted by workers and people living in the vicinity of the workplace, and it can develop into environmental problems.

  As described above, a method disclosed in Patent Document 2 has been proposed as a technique for obtaining a natural dye of indigo dyeing without passing through “sukumo”. In this method, indigo leaves are frozen and thawed and then dried to produce a natural dye for indigo dyeing. However, this method does not completely hydrolyze indigo leaves by controlling the temperature of the indigo leaves. In addition, in order to oxidize, the leaves have to be spread out in a flat shape so that the whole is in contact with the air. In addition, in order to spread the indigo leaves, a large area is required, and there is a problem that they cannot be processed in large quantities. In order to expand the use of indican plants, it is important to treat a large amount of indican plants efficiently in a short period of time, and treatment methods for indican plants that can meet these requirements are still in practical use. It has not been.

Disposal of waste and residues such as leaves and stems generated when harvesting fresh leaves and producing useful substances is also a problem. Due to the limitations of waste disposal capacity in recent years, demands for reuse and recycling are increasing, and methods for treating products and methods using waste that meet such demands have been sought.
Japanese Patent Laid-Open No. 10-331077 JP 2003-192934 A

  The present invention has been made to solve such problems. The main object of the present invention is to effectively produce a useful substance obtained from a raw leaf of an indican plant, as an additive that can also be used for applications other than dyeing. An object of the present invention is to provide a method for producing an additive containing indigo, indirubin, or isatin, in which generated waste residue is extremely reduced.

  In order to achieve the above object, a method for producing an additive containing indigo, indirubin or isatin according to claim 1 of the present invention includes a step of freezing freshly cut leaves and thawing frozen fresh leaves. A step of adding an enzyme β-glucosidase to the enzyme present in the thawed raw leaf as necessary, hydrolyzing the contained indican, oxidizing the hydrolyzed indoxyl, and oxidation And a step of adjusting the water content of the raw leaves containing indigo, indirubin or isatin to obtain a mud or paste.

  The method for producing an additive containing indigo, indirubin or isatin according to claim 2 includes a step of freezing the harvested fresh leaves, a step of thawing the frozen fresh leaves, and an enzyme present in the thawed fresh leaves. If necessary, an enzyme β-glucosidase is added, a step of hydrolyzing the contained indican, a step of oxidizing the hydrolyzed indoxyl, and a fresh leaf containing indigo, indirubin or isatin obtained by the oxidation. Further, the method includes a step of decomposing the cell wall by cellulase or an enzyme treatment containing cellulase, and a step of adjusting the water content of the raw leaves from which the cell wall has been decomposed to obtain a mud or paste.

  Furthermore, the method for producing an additive containing indigo, indirubin or isatin according to claim 3 includes a step of freezing the harvested fresh leaves, a step of thawing the frozen fresh leaves, and an enzyme present in the thawed fresh leaves. If necessary, the enzyme β-glucosidase is added to hydrolyze the contained indican, the step of oxidizing the indoxyl produced by hydrolysis, and the fresh leaves containing indigo, indirubin or isatin obtained by oxidation And a step of adjusting the water content of the crushed fresh leaves to form a mud or paste.

  Furthermore, the method for producing an additive containing indigo, indirubin or isatin according to claim 4 is a method for producing an additive containing indigo, indirubin or isatin according to any one of claims 1 to 3, A step of further drying the mud or paste-like additive to remove moisture and making it into powder by a pulverizer is provided.

  Furthermore, the method for producing an additive containing indigo, indirubin or isatin according to claim 5 is a method for producing an additive containing indigo, indirubin or isatin according to any one of claims 1 to 4, The step of freezing the fresh leaves is held at a temperature at which the leaf cells are destroyed to freeze and expand the moisture contained in the fresh leaves.

  Furthermore, the method for producing an additive containing indigo, indirubin or isatin according to claim 6 is the method for producing an additive containing indigo, indirubin or isatin according to claim 5, wherein the raw leaves are frozen. The process is managed at a temperature of −5 to −20 ° C. for 24 to 72 hours.

  Furthermore, the method for producing an additive containing indigo, indirubin or isatin according to claim 7 is the method for producing an additive containing indigo, indirubin or isatin according to claim 5, wherein the raw leaves are frozen. The process is managed at a temperature of approximately −15 ° C. for approximately 32 hours.

  Furthermore, the method for producing an additive containing indigo, indirubin or isatin according to claim 8 is a method for producing an additive containing indigo, indirubin or isatin according to any one of claims 1 to 7, In the step of thawing the fresh leaves, the enzyme β-glucosidase is added to the enzymes present in the fresh leaves as needed, and the time for the hydrolysis of indican contained in the fresh leaves is maintained at a temperature at which the enzyme activity increases.

  Furthermore, the method for producing an additive containing indigo, indirubin or isatin according to claim 9 is the method for producing an additive containing indigo, indirubin or isatin according to claim 8, wherein the raw leaves are thawed. The process is performed at 4 ° C to 50 ° C.

  Furthermore, the method for producing an additive containing indigo, indirubin or isatin according to claim 10 is a method for producing an additive containing indigo, indirubin or isatin according to any one of claims 1 to 9, The process of oxidizing hydrolyzed indoxyl is either a method of oxidizing with air or air with oxygen or air, a method of oxidizing by reducing the water content by cold air or hot air drying, or a method of physical or chemical oxidation It is.

  Furthermore, the method for producing an additive containing indigo, indirubin or isatin according to claim 11 is a method for producing an additive containing indigo, indirubin or isatin according to any one of claims 1 to 10, The raw leaves to be cut are cut at positions near the tip of the plant body, and the cut fresh leaves include the leaves and stems of the tip.

  According to the method for producing an additive containing indigo, indirubin or isatin of the present invention, mud, powder, including an additive containing a useful substance of fresh leaves, regardless of a conventionally used storage method, It can be made into a form that can be used as an additive such as paste or powder. In particular, labor and efficiency of cutting and drying fresh leaves into small pieces can be saved, and a large amount of fresh leaves can be processed in a short period of time without requiring days or high technology for production. The excellent feature of efficient and inexpensive production can be realized. Further, in the present invention, the harvested and collected fresh leaves can be stored and processed without being influenced by the weather, so that stable production and supply can be realized. In addition, it is possible to realize a production method free from collection residue by using leaves and stems.

  Furthermore, the additive obtained by this method can be used to bring out the functions of the plant. Additives made from fresh leaves are non-chemical natural materials that are safe and have no harmful effects on the human body, such as coloring by chemical substances. In addition, various effects can be obtained in addition to functions depending on the plant, and the use can be expanded as a safe and highly functional natural additive, which can contribute to increased production of plants, increased demand, and activation of the production area.

  Furthermore, the yield can be increased by improving the mowing method, and there is an advantage that high-quality raw leaves can be obtained efficiently.

  Embodiments of the present invention will be described below with reference to the drawings. However, in order to embody the technical idea of the present invention, the following embodiment exemplifies a method for producing an additive containing indigo, indirubin or isatin, and the present invention is indigo, indirubin or The manufacturing method of the additive containing isatin is not specified as follows.

  Further, the present specification by no means specifies the members shown in the claims to the members of the embodiments. In particular, the dimensions, materials, shapes, relative arrangements, and the like of the component parts described in the embodiments are not intended to limit the scope of the present invention unless otherwise specified, and are merely explanations. It's just an example. Note that the size, positional relationship, and the like of the members shown in each drawing may be exaggerated for clarity of explanation. Furthermore, in the following description, the same name and symbol indicate the same or the same members, and detailed description thereof will be omitted as appropriate. Furthermore, each element constituting the present invention may be configured such that a plurality of elements are constituted by the same member and the plurality of elements are shared by one member, and conversely, the function of one member is constituted by a plurality of members. It can also be realized by sharing.

  FIG. 1 shows a method for producing an additive containing a useful substance obtained from an indican plant according to an embodiment of the present invention. In this case, the indican-containing plant is Tadeai, and the indican contained in this raw leaf is acted on by the enzyme contained in the raw leaf or by adding the enzyme β-glucosidase as necessary, and hydrolyzed to indoxyl. Disassembled. This is oxidized to produce indigo indigo. By changing the conditions for oxidation, orange isatin and magenta indirubin can be obtained. As shown in FIG. 1, the additive containing a useful substance obtained from Tadeai is indigo obtained through a hydrolysis and oxidation process after a Tadeai mowing process, a freezing process for storage, a thawing process, The additive containing indirubin and isatin can be used as a dye by various treatments, as paper, Japanese paper, wallpaper, as a filler mixed in plaster, etc., or as a food added to food.

[Reaping]
Hereinafter, a procedure for producing an additive containing a useful substance obtained from a raw leaf of an indican plant will be described in order. First, the fresh leaves are cut and harvested at a position near the tip of the grown indican plant. In the case of Tadeai, conventionally, the root and about 10 to 15 cm have been cut from the above-ground part. However, it takes too many days to cultivate Tadeai, and high quality Tadeai leaves and stems cannot be taken. Also, the leaves that are closer to the ground are often withered because they are present from the early stages of growth. On the other hand, the tip part is a new leaf and contains high-quality ingredients. In particular, it has been reported that the sprout at the tip part has almost the same content and quality of indican as compared to the traditionally cut tadeai, or that the sprout has better quality indican. In addition, by cutting near the tip, the side buds grow and the subsequent growth also increases the yield quickly. Therefore, by harvesting only the fast-growing part of the tip of the Tadeai, leaves and stems containing good quality indican and enzyme components can be harvested, and the harvested part has been regenerated, so that it can be re-harvested in a short period of time. Yes, you can increase the yield. Specifically, it is cut and collected at a position of about 10 to 20 cm, preferably about 10 to 15 cm from the tip of the tadeai leaf / stem. Since the tip portion regenerates quickly, the tadeai that has been harvested as the most mowing is easy to regenerate and grow, so that the second trimming operation can be performed early and the production efficiency is improved. In addition to the vertical growth of Tadeai, it is also possible to harvest shoots, leaves and stems that have grown in the horizontal direction. Other side buds also increase at the tip, resulting in new leaves and increased yield. In this way, the harvest amount can be increased by sequentially repeating the harvesting and regenerative growth of tadeye, and a large number of good quality tadeye leaves can be obtained in a short period of time. In the experiment by the present inventors, the above method was able to obtain a 50% increase in yield from the same field as compared to the conventional method in which the tadeye was grown and harvested. The raw leaves of the taeyei cut and harvested as described above are cut or cut into a size of about 1 to 2 cm.

[frozen]
Immediately after harvesting Tadeai fresh leaves, store them in a plastic net bag or container box with good air and cold air permeability and store them in a freezer or low-temperature refrigerator. This is because the cells contained in the cut leaves are frozen by freezing the water. As a result of the comparison by the inventors of the present invention in the range of −3, −5, −15, −20, and −35 ° C. for 24 to 72 hours frozen storage, the storage temperature was −3 to −20 ° C., preferably − The temperature is controlled at 5 to -15 ° C, more preferably about -15 ° C, and the time is set to 12 to 72 hours, preferably about 32 hours, so that the thawing process can be performed relatively quickly, and the raw leaves of Tadeai discolor. It has been found. Instant cooling and freezing using liquid nitrogen are possible, but an existing electric freezer is preferable from the viewpoint of safety and cost.

[Thawing]
Next, the leaves of the tadeye that have been stored frozen for a predetermined period are taken out and thawed. Thawing is performed by natural thawing, cold air, or hot air thawing. Indigo is effectively hydrolyzed by enzymes, and indigo is oxidized by contact with air, because cells are destroyed when placed in a room temperature and a warm state from a frozen state. To change. Specifically, in the process of thawing from the frozen state, indican contained in the raw leaves of Tadeai is hydrolyzed to indoxyl by the action of enzymes and moisture in the plant, and indoxyl is oxidized by oxygen in the air. Indigo is generated. Indirubin and isatin are also produced when the oxidation conditions are changed. The useful substance thus obtained is used as an additive in the form of mud or paste using an enzyme, a grinder or the like.

[Enzyme treatment]
First, the enzyme treatment will be described with reference to FIG. Tadeai taken out from the refrigerator 1 is thawed and hydrolyzed by an enzyme contained in the living body or by adding an enzyme β-glucosidase as necessary. Further, tadeai leaves sufficiently oxidized by oxygen in the air are put into the dissolution tank 2 and mixed with cellulase supplied from the enzyme supply unit 3 or an enzyme containing cellulase. The dissolution tank 2 is provided with a stirring propeller 4, and the stirring propeller 4 is rotated to cause a reaction. The stirring time is several hours. By such an enzyme treatment, the leaf of Tadeai is made into a single cell and made into a paste or mud. Further, the obtained liquid component is filtered through the filter 5 to obtain a paste-like additive from which unnecessary components are removed. As the filter 5, a membrane filter (Millipore filter), a porous material, or the like can be used. Further, a method such as centrifugal separation may be used instead of filtration with a filter.

[Mechanical processing]
In addition to making Tade indigo cells by enzymatic treatment, it can be mechanically and physically decomposed. Based on FIG. 3, a method for mechanically crushing the tadeai to obtain the additive will be described. In this method, Tadeai taken out from the refrigerator 1B is thawed and hydrolyzed with an enzyme contained in the living body. Furthermore, the leaf of the tapeye that has been sufficiently oxidized by oxygen in the air is ground by a crusher 6 or a grinder to form a paste. The crusher 6 can use a masco loader or a stone mill. The masco loader shown in FIG. 3 rotates the upper and lower crushing parts in the reverse direction, and mechanically grinds the tether eyes inserted therebetween. Then, the ground powder is filtered through the filter 5B in the same manner as described above to obtain a paste-like or mud-like additive.

  The above-described method does not require the labor required for the production of soup, and can be processed in a short period of time by saving labor without producing dried leaf indigo. Furthermore, batch processing is possible, and the additive can be mass-produced by continuous processing.

  The paste-like additive obtained by enzyme treatment or mechanical treatment as described above can be used as a dye as a substitute for soot. For example, in addition to dyeing clothes, hats, gloves, socks, and the like as dyes for fibers of clothing and fabrics, they can be used as dyes for wood, wood boards, paper, and the like. Alternatively, it is possible to provide the effect of Tadeai by filling and mixing the additive material in the form of mud or paste in accordance with the product application. For example, it can be used as a raw material for paper itself or as a composite product of Japanese paper or non-wood paper. In particular, the method using an enzyme treatment can easily break down fibers and mix them with paper.

  Further, the paste-like additive can be made into a powdery, granular or powdery form. The paste-like additive is dried to remove moisture, further refined by a pulverizer, and subjected to particle size selection through a sieving machine, whereby a granular, powdery or powdery additive is obtained. Handling such powder, granule, powder, etc. facilitates handling, and mixing and mixing into the material can be performed more easily. For example, it can be used as a dye for fibers, wood, paper, etc., and it can be mixed with plaster, white cement, ink, paper clay, etc. It can be made high quality by adding functions such as ultraviolet absorption. Alternatively, it can be mixed with foods such as frozen confectionery, Japanese confectionery, jelly and seafood surimi, and used as foodstuffs to add color, flavor, or medicinal effects. Thus, the additive produced from Tade indigo can be used in combination with various products and products.

  Especially for building materials, since so-called sick house syndrome due to chemical substances has become a problem in recent years, building materials made of natural materials that do not use chemical substances harmful to the human body are required. Therefore, the above-mentioned additive can be applied to the board as a powder, filled into plaster or diatomaceous earth bond as an additive, wallpaper or tapestry can be produced, or used as a filler or extender for other members. As a result, fire resistance, insect resistance, UV absorption, deodorant, etc., which have low thermal conductivity and are effective against fire, can be used effectively as building materials, and even for people who use natural non-chemical materials. Can be suitably used as a gentle material.

  Or it can also utilize as an additive which defibrated as it is, without decomposing | disassembling the frozen and thawed cyanogen-containing plant irrespective of the enzyme treatment and mechanical treatment which were mentioned above. This method is particularly suitable for use as paper. For example, in a defibrated state, it is mixed with various necessary materials such as adding paste or filling with fiber material in the same way as the conventional paper manufacturing method, and after having been pooled and poured, dried to make paper . In experiments conducted by the present inventor, it was possible to obtain several tens to hundreds of filling amounts by this method, and it was possible to obtain Japanese paper, non-wood paper, wallpaper and the like. Unlike conventional paper, these paper products have the sterilization and antiseptic effects described above, and can be used as high-value-added products excellent in color, texture, functionality, and the like.

  In this way, Tadeai can be used for purposes other than dyeing applications, and its applications and possibilities are expanded. Furthermore, the manufacturing method of the additive containing useful substances saves laborious processes such as the production of scum, saves labor, and makes it easy and inexpensive to manufacture. Therefore, the waste disposal problem that has occurred in the past can be solved by using up all the harvest. This makes it possible to produce products that are environmentally friendly with no waste residues.

  Next, the utilization form of the additive containing the useful substance obtained from the raw leaves of Tade indigo by the above method will be described. For example, it can be added to general industrial applications that can be added to paper, Japanese paper, non-woven fabric, plaster, diatomite board, etc., or high quality applications such as food bases, frozen desserts, soap (body soap), and lip balm.

[paper]
When using for paper, first, NBKP (coniferous bleached kraft pulp) beaten to 600 ml by Canadian standard freeness (CFS: pulp freeness test method according to JIS-P8121) The powdered additive was blended so as to be 0 to 60% based on the total weight. A 3.0% by weight latex paper strength enhancer (Aika Ivon RAX117) was added thereto and stirred to prepare a uniform dispersed slurry. While stirring the dispersion slurry, paper is made according to JIS-P8209 using a square sheet machine (25 cm × 25 cm), pressed at a pressure of 3.5 kgf / cm ² , and dried at about 100 ° C. with a rotary dryer. Thus, an additive-containing paper was prepared. The obtained paper was colored with an additive, and was a high-quality paper material excellent in antibacterial properties and flame retardancy.

[Japanese paper]
When used for Japanese paper, several to several hundred parts of the additive obtained by the above method are mixed and blended with the raw materials such as cocoon, cocoon, and husk, and the mixture is sown in accordance with the concentration of the paste material. In addition, tree-free paper can be obtained by collecting paper quality from recycled paper such as milk cartons and carrying out mixing and blending similar to the above. Here, 100 g of straw, 100 g of a muddy additive, 20 g of glue, and 30 L of water were stirred and mixed, and papermaking was performed. Further, 100 g of straw, 200 g of a mud-like additive, 15 g of paste material, and 25 L of water were stirred and mixed, and papermaking was performed in the same manner. The obtained Japanese paper was colored with an additive, and was a high quality Japanese paper excellent in antibacterial properties and flame retardancy.

[cloth]
When weaving a cloth with Japanese paper as a thread, a mud or paste-like additive is squeezed into a sheet, cut into slits (slits), and twisted into a thread. It can be woven or knitted by using yarn. The obtained cloth was colored with an additive, and was a high-quality cloth excellent in antibacterial properties and flame retardancy.

[Nonwoven fabric]
In addition, in order to make a nonwoven fabric, fibers and pulp are arranged in a vertical direction with a card machine, or entangled into a sheet, and a chemical adhesive mixed with powdered additives is sprayed or applied and dried. To create a wet nonwoven fabric. A mask, a filter, etc. are created using this nonwoven fabric. The obtained non-woven fabric was colored with an additive, and was a high-quality non-woven fabric excellent in antibacterial properties and flame retardancy.

[Stucco]
In order to use it as an additive for plaster, an appropriate amount of a mud or powder additive is added to the plaster for interior, and the mixture is stirred and mixed while adjusting the viscosity and hue, and this plaster is applied to the wall and sprayed. The obtained plaster was colored with an additive, and could be a high-quality plaster excellent in antibacterial properties and flame retardancy.

[Diatomite board]
In order to use for diatomite board, diatomite, natural zeolite, aggregate, fiber, lime, and mud-like additives are blended, mixed with stirring, poured into a mold and dried. The obtained diatomite board was colored with an additive, and was a high-quality diatomite board excellent in antibacterial properties and flame retardancy. As described above, additives containing useful substances can be used for general industrial applications.

[Food base material]
Next, an example in which an additive is used as a food will be described. First, as a pizza dough, 250 g of weak flour and 250 g of strong flour are mixed with 20 to 50 g of powder additive uniformly, and 2 teaspoons of sugar, 1 teaspoon of salt, 2 teaspoons of dry yeast, and 2 tablespoons of olive oil are added. Bake it. Then, 1 kg of wheat flour, 8 teaspoons of dry yeast, and 50 g of powder additives are mixed well, and 2 tablespoons of olive oil, 1 tablespoon of sugar, a little salt and a little lemon are added to make a dough. A pizza dough colored in blue and red was obtained by the flavonoid and chlorophyll decomposition and removal processes. This pizza dough can be made a beautiful blue color in addition to the medicinal effect due to the inclusion of the additive. Conventionally, there was no blue food. Recent studies have reported that blue foods have a dieting effect that reduces appetite. Therefore, by using the colorant of the food base material or food additive obtained in the present embodiment, in addition to the design fun of making it blue by the flavonoid and chlorophyll decomposition and removal processes, the diet It can have a function as a food and can increase the added value of the food base or additive.

  Similarly, okonomiyaki was prepared. In this example, 160 g of wheat flour was mixed well with 10 g to 30 g of powder additive, 5 to 7 eggs were added to make dough, and then okonomiyaki was prepared according to a normal method. Similar to the above, this okonomiyaki can be made blue and red by the flavonoid and chlorophyll decomposition and removal processes, and has a diet effect that reduces appetite in addition to the medicinal effects.

  Further, when preparing agar jelly, prepare 5 g of powdered gelatin, an appropriate amount of powdered additive, 300 cc of water, and 2 tablespoons of sugar, put powdered gelatin in water and shake it up. Then, the powder additive is stirred in water. Filter with a cloth and add a certain amount of sugar. Disperse well while warming, add gelatin and mix. Add 10% of the powdered additive to the mushroom and boil it for about 10 minutes. Store the agar that has been solidified in the refrigerator. Furthermore, 3-6 g of powder agar, an appropriate amount of powder additives, plain yogurt, milk, sugar and amaretto were added to make a jelly. The viewpoint jelly thus obtained is also blue and red by the flavonoid and chlorophyll decomposition and removal processes as described above, and has a diet effect that reduces appetite in addition to the medicinal effect.

  Furthermore, when making indigo rice cake, indigo dango, indigo arare, kneaded powder 160 g, white egg flour 40 g, appropriate amount of powder additives, 60 g sugar, 200 cc hot water were added and kneaded, dried and baked at low temperature and allowed to stand. As described above, indigo arabe obtained in this way is blue and red by the flavonoid and chlorophyll decomposition and removal processes, and has a diet effect that reduces appetite in addition to medicinal effects.

  In addition, in the case of indigo dressing soup, 2 tablespoons of olive oil, 1 tablespoon of flour, appropriate amount of paste-like additive, a little lemon, 1 piece of garlic, salt and pepper were added and warmed over low heat. This dressing sauce is also blue and red by the flavonoid and chlorophyll decomposition and removal processes as described above, and has a diet effect that reduces appetite in addition to the medicinal effect.

  Furthermore, in the case of indigo sesame ice, it was prepared by mixing 4 tablespoons of paste-like additive, appropriate amount of white sesame seeds, fresh cream, 6 tablespoons of granulated sugar, milk and egg yolk and putting them in a freezer. This sesame-containing ice is also blue and red in the flavonoid and chlorophyll decomposition and removal processes, as described above, and has a diet effect that reduces appetite in addition to medicinal effects.

Furthermore, in the case of fresh leaf tea, it was prepared by drying fresh leaf leaves and pouring the powder additive into hot water. This fresh leaf tea is also blue and red by the flavonoid and chlorophyll decomposition and removal processes as described above, and has a diet effect that reduces appetite in addition to medicinal effects.
As described above, by using an additive containing a useful substance using raw leaves of an indican plant obtained by the method according to the present embodiment as a food material, in addition to a coloring effect, as a preservative due to an antibacterial action It can be a high-value-added product to which an effect or a medicinal effect such as a detoxification action or a diet effect is added. In particular, Tade indigo obtained by the conventional method has a problem that it is not safe for food use because it contains miscellaneous bacteria and the like even if an intermediate product such as scum is used as an additive. According to such a method, impurities such as germs are excluded from the additive containing the useful substance obtained as a result of the treatment, so that it can be used safely as a food.

[Soap]
In addition to the above, the additive obtained by the method according to the present embodiment can be applied to a facial cleanser, a drug, and the like. First, in an embodiment applied to soap, olive oil 200 cc, coconut oil 100 cc, palm oil 80 g, avocado oil 100 g, beeswax 20 g, caustic soda 60 g, purified water 170 cc, and powder additives are prepared in appropriate amounts. In a hot water bath, dissolve the beeswax, and stir the powder additive to stir well with stirring. Further dilute caustic soda in purified water and stir while pouring into a bowl. When a lump (viscosity) came out, it was placed in a mold and allowed to cool to prepare a soap bar. In addition, liquid soap (body soap) was prepared by adding potassium hydroxide and glycerin diluted with purified water to animal fats and oils. In addition to being colored blue and red in the flavonoid and chlorophyll decomposition and removal processes, the soap obtained in this way has a high medicinal effect and is effective in antiviral properties and suppression of Candida. In addition, because it uses natural materials, it is useful for atopic dermatitis.

[Bath preparation]
Moreover, in the Example applied to a bath agent, dry sodium sulfate, sodium hydrogencarbonate, sodium chloride, a powder additive, a proteolytic enzyme, a fragrance | flavor, chamomile, and anhydrous silicic acid are mix | blended. Alternatively, it is prepared by blending potassium chloride, sodium chloride, sodium bicarbonate, dry sodium sulfate, powder additives, perfume, and anhydrous silicic acid. The bath preparation thus obtained is also colored in blue and red by the flavonoid and chlorophyll decomposition and removal processes in the same manner as described above, and has high medicinal effects, antiviral properties, suppression of Candida, etc. In addition, it is useful for atopic dermatitis because it uses natural materials.

[Lip balm]
Furthermore, in the Example applied to a lip balm, 3-4 cups of castor oil, 1/2 cup of candelilla wax, 1/8 cup of menthol crystal, and a small amount of powder additives were added and melted and solidified. Or add the oil or natural mint flavor oil as appropriate. The lip balm thus obtained also has a high medicinal effect in addition to being colored blue and red by the flavonoid and chlorophyll decomposition and removal processes in the same manner as described above, and has antiviral properties, suppression of Candida, etc. In addition, it is useful for atopic dermatitis because it uses natural materials. As described above, high quality and high functionality can be realized by applying the additive material containing the useful substance obtained by the method according to the present embodiment.

  As described above, the present invention mainly adds the enzyme β-glucosidase to the enzyme contained in the raw leaves as necessary, by examining the conditions of the freezing and thawing process for indican contained mainly in the raw leaves, thereby promptly hydrolyzing. Indigo, indirubin and / or isatin, etc. that become dyes by proceeding and further performing air oxidation such as air ring with oxygen or air, oxidation accompanying reduction in moisture content, physical and chemical oxidation Produces useful substances.

  The method for producing an additive containing indigo, indirubin or isatin obtained by the present invention can be suitably used for wallpaper, plaster, diatomaceous earth bond, interior materials such as additives mixed in wall materials, Japanese paper, and the like. In addition, it can be used as a food, as well as on paper, and as an ancient indigo dye.

It is a conceptual diagram which shows the manufacturing method of the additive containing indigo, indirubin, or isatin obtained from the raw leaf of an indican plant containing an embodiment of the present invention. It is a conceptual diagram which shows the manufacturing method of the additive containing indigo, indirubin, or isatin obtained from the raw leaf of an indican plant by enzyme treatment. It is a conceptual diagram which shows the manufacturing method of the additive containing indigo, indirubin, or isatin obtained from the raw leaf of an indican plant by mechanical processing.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1, 1B ... Freezer 2 ... Dissolution tank 3 ... Enzyme supply part 4 ... Stirring propeller 5, 5B ... Filter 6 ... Crusher

Claims (11)

Freezing the harvested fresh leaves;
Thawing frozen raw leaves;
Adding an enzyme β-glucosidase to the enzyme present in the thawed raw leaves as necessary, and hydrolyzing the contained indican;
Oxidizing the hydrolyzed indoxyl; and
A step of adjusting the water content of fresh leaves containing indigo, indirubin or isatin obtained by oxidation to form a mud or paste; and
A method for producing an additive containing indigo, indirubin or isatin, comprising: Freezing the harvested fresh leaves;
Thawing frozen raw leaves;
Adding an enzyme β-glucosidase to the enzyme present in the thawed raw leaves as necessary, and hydrolyzing the contained indican;
Oxidizing the hydrolyzed indoxyl; and
A step of degrading a cell wall by indigo, indirubin or isatin obtained by oxidation and further cellulase or enzyme treatment containing cellulase,
Adjusting the water content of the raw leaves whose cell walls have been decomposed to form a mud or paste,
A method for producing an additive containing indigo, indirubin or isatin, comprising: Freezing the harvested fresh leaves;
Thawing frozen raw leaves;
Adding an enzyme β-glucosidase to the enzyme present in the thawed raw leaves as necessary, and hydrolyzing the contained indican;
Oxidizing the indoxyl produced by hydrolysis; and
A step of mechanically crushing fresh leaves containing indigo, indirubin or isatin obtained by oxidation with a crusher;
Adjusting the water content of the crushed fresh leaves to make a mud or paste,
A method for producing an additive containing indigo, indirubin or isatin, comprising: A method for producing an additive containing indigo, indirubin or isatin according to any one of claims 1 to 3,
A method for producing an additive containing indigo, indirubin or isatin, further comprising a step of further drying the mud or paste-like additive to remove moisture and making the powder into a powder by a pulverizer. A method for producing an additive containing indigo, indirubin or isatin according to any one of claims 1 to 4,
The method for producing an additive containing indigo, indirubin or isatin, wherein the step of freezing the fresh leaves is held at a temperature at which the leaf cells are destroyed to freeze and expand the water contained in the fresh leaves. A method for producing an additive containing indigo, indirubin or isatin according to claim 5,
The method for producing an additive containing indigo, indirubin or isatin, wherein the step of freezing the fresh leaves is managed at a temperature of -5 to -20 ° C for 24 to 72 hours. A method for producing an additive containing indigo, indirubin or isatin according to claim 5,
The method for producing an additive containing indigo, indirubin or isatin, wherein the step of freezing the fresh leaves is controlled at a temperature of about -15 ° C for about 32 hours. A method for producing an additive containing indigo, indirubin or isatin according to any one of claims 1 to 7,
The step of thawing the fresh leaves adds enzyme β-glucosidase to the enzyme present in the fresh leaves as necessary, and maintains the time for indican contained in the fresh leaves to be substantially hydrolyzed at a temperature at which the enzyme activity becomes high. A method for producing an additive containing indigo, indirubin or isatin, which is characterized. A method for producing an additive containing indigo, indirubin or isatin according to claim 8,
The method for producing an additive containing indigo, indirubin or isatin, wherein the step of thawing the fresh leaves is performed at 4 ° C to 50 ° C. A method for producing an additive containing indigo, indirubin or isatin according to any one of claims 1 to 9,
The process of oxidizing hydrolyzed indoxyl is either a method of oxidizing with air or air with oxygen or air, a method of oxidizing by reducing the water content by cold air or hot air drying, or a method of physical or chemical oxidation The manufacturing method of the additive containing indigo, indirubin, or isatin characterized by these. A method for producing an additive containing indigo, indirubin or isatin according to any one of claims 1 to 10,
The method for producing an additive containing indigo, indirubin, or isatin, wherein the raw leaves to be cut are cut at a position near the tip of the plant body, and the cut raw leaves include leaves and stems of the tip.

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