JP2005207938A - 検出素子及び検出方法 - Google Patents
検出素子及び検出方法 Download PDFInfo
- Publication number
- JP2005207938A JP2005207938A JP2004015974A JP2004015974A JP2005207938A JP 2005207938 A JP2005207938 A JP 2005207938A JP 2004015974 A JP2004015974 A JP 2004015974A JP 2004015974 A JP2004015974 A JP 2004015974A JP 2005207938 A JP2005207938 A JP 2005207938A
- Authority
- JP
- Japan
- Prior art keywords
- substance
- specimen
- fluid
- flow
- different
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0636—Focussing flows, e.g. to laminate flows
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25625—Dilution
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Optics & Photonics (AREA)
- Medicinal Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
【解決手段】 検体中の複数の異なる物質を検出するための検出素子であって、基体に設けられ、流体の複数の流れの層を形成可能な流路と、前記流路中に設けられ、前記検体中の複数の異なる物質を各々捕捉するための複数の異なる物質捕捉部10、11とを備え、前記複数の異なる物質捕捉部10、11は、流体の複数の流れの層71、72に分れて分布し、流体と捕捉された物質との作用により検体中の各々の物質に対する独立の情報が得られるように配置されている検出素子。
【選択図】 図2
Description
前記検体中の複数の異なる物質は標識を有し、前記複数の流れの層を流れる流体は前記標識と作用することにより作用物を放出させるための流体であることが好ましい。
前記標識が触媒作用を有する物質、電気化学的に発光する物質または蛍光物質であることが好ましい。
本発明の検出素子は、検体中の複数の異なる物質を検出するための検出素子であって、基体に設けられ、流体の流れの層(以降、層状流と記す)を複数形成可能な流路と、前記物質を特異的に捕捉するために前記流路中に設けられた、複数の異なる物質捕捉部とを備え、前記物質捕捉部は、前記層状流の一方の層を流れる流体を切り替えることにより、前記物質に対する独立の情報が得られるように配置されていることを特徴とする。
物質捕捉部は、流路の複数の層状流の一方を流れる流体を切り替えることにより、物質に対する独立の情報が得られるように配置されている。ここで、層状流の一方の層を流れる流体とは、検体中に含まれる物質の有する標識と作用することにより、作用物を放出させるための流体(基質)であり、その放出された作用物を検出することにより物質捕捉部に捕捉された検体中の物質の量等の情報を得ることができる。
検体中の物質の検出については、例えば、物質捕捉部に捕捉された検体と、物質特異的な2次抗体に酵素標識したものとの複合体を形成せしめ、物質捕捉部ごとに層状流を流れる流体を用いて酵素標識と作用する酵素基質を選択的に供給し、生成した酵素反応生成物(作用物)を物質捕捉部の下流に位置する検出用電極により検出する。
また、物質捕捉部に形成せしめる複合体は、触媒標識された2次捕捉体の他に、検体と2次捕捉体との複合体に対して特異的に結合する3次以降の触媒標識抗体でもよい。
触媒作用を有する物質としては、グルコース酸化酵素、コリン酸化酵素、ラクトース酸化酵素、西洋ワサビペルオキシダーゼが挙げられる。それぞれ、基質として、グルコース、コリン、ラクトース、ルミノールと過酸化水素が挙げられる。電気化学発光物質としては、トリス(ビピリヂル)ルテニウムが挙げられる。蛍光標識物質としては、5−カルボキシフルオレセイン、Quene−1(8−アミノ−2−(トランス−2−アミノスチリル)−6−メトキシキノリン−N,N,N’,N’−テトラ酢酸テトラナトリウム塩)、BCECF(2’,7’−ビス(カルボキシエチル)−4または5−カルボキシフルオレセイン)が挙げられる。
実施例1
図1は、本発明の一実施の形態におけるバイオセンサの構造を示すものである。ここでは、触媒作用を有する物質として、グルコース酸化酵素(GOX)を用いる。また、測定対象とする検体として、ヒトαフェトプロテイン(AFP)及びヒトβ2マイクログロブリン(β2MG)を用いる。
図7のような構造のバイオセンサを作製する。基板としてガラス基板を用いる。図9に示すような幅100μm、深さ50μm微小流路48をウェットエッチング法により形成する。実施例1に於いて形成した層状流を更に厳密に検出し、基質の拡散を抑制し、物質捕捉部をより近接して形成できるようにする。これにより更なる物質捕捉部の高密度化が可能となる。
実施例1、2にある系において、酵素標識の代替として、蛍光標識用いることができる。
層状流により流体のpHを特定の1層のみ変更し、蛍光標識の蛍光特性が変化することで、被検体の区別が可能となる。特定波長における蛍光強度あるいは蛍光スペクトルの変化を検出する。3次抗体の標識としてpH感受性蛍光色素5−カルボキシフルオレセインを用いて、実施例2と同様の検出が可能となる。
3 ドレイン
4,5,6 電極パッド
7 基板
9 微小流路
10、11 物質捕捉部
13 PDMS基板
14 微小流路
15 ガラス基板
16,17,18 薄膜電極
19,20,21 電極パッド
22,23,24 貫通孔
32,33,34 電極パッド
35 微小流路
36、37、38 インレット
39 ドレイン
40 基板
42,43 物質捕捉部
44、45 界面
46 疎水化処理部
47 基板
48 微小流路
49 ガラス基板
50、51、52、53 貫通孔
71、72 層状流
Claims (5)
- 検体中の複数の異なる物質を検出するための検出素子であって、基体に設けられ、流体の複数の流れの層を形成可能な流路と、前記流路中に設けられ、前記検体中の複数の異なる物質を各々捕捉するための複数の異なる物質捕捉部とを備え、前記複数の異なる物質捕捉部は、流体の複数の流れの層に分れて分布し、流体と捕捉された物質との作用により検体中の各々の物質に対する独立の情報が得られるように配置されていることを特徴とする検出素子。
- 前記情報を検出するための検出部を更に備えていることを特徴とする請求項1記載の検出素子。
- 前記検体中の複数の異なる物質は標識を有し、前記複数の流れの層を流れる流体は前記標識と作用することにより作用物を放出させるための流体であることを特徴とする請求項1または2記載の検出素子。
- 前記標識が触媒作用を有する物質、電気化学的に発光する物質または蛍光物質であることを特徴とする請求項1乃至3のいずれかに記載の検出素子。
- 検体中の複数の異なる物質を検出するための検出方法であって、前記複数の異なる物質の各々を特異的に捕捉するための複数の異なる物質捕捉部を有する流路に前記検体を導入し、物質捕捉部で物質を捕捉する工程と、前記流路内に流体が流れる複数の流れの層を形成する工程と、前記複数の流れの層を切り替えて流体を流すことにより、流体と捕捉された物質との作用により検体中の各々の物質に対する独立の情報を得る工程とを有することを特徴とする検出方法。
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004015974A JP3991034B2 (ja) | 2004-01-23 | 2004-01-23 | 検出方法 |
DE602005012674T DE602005012674D1 (de) | 2004-01-23 | 2005-01-18 | Nachweiselement und nachweisverfahren |
PCT/JP2005/000811 WO2005071393A2 (en) | 2004-01-23 | 2005-01-18 | Detecting element and detection method |
EP05709265A EP1711827B1 (en) | 2004-01-23 | 2005-01-18 | Detecting element and detection method |
US10/554,219 US7863051B2 (en) | 2004-01-23 | 2005-01-18 | Detecting element and detection method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004015974A JP3991034B2 (ja) | 2004-01-23 | 2004-01-23 | 検出方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2005207938A true JP2005207938A (ja) | 2005-08-04 |
JP3991034B2 JP3991034B2 (ja) | 2007-10-17 |
Family
ID=34805472
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2004015974A Expired - Fee Related JP3991034B2 (ja) | 2004-01-23 | 2004-01-23 | 検出方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US7863051B2 (ja) |
EP (1) | EP1711827B1 (ja) |
JP (1) | JP3991034B2 (ja) |
DE (1) | DE602005012674D1 (ja) |
WO (1) | WO2005071393A2 (ja) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007315753A (ja) * | 2006-05-23 | 2007-12-06 | Univ Of Electro-Communications | マイクロ化学チップ装置 |
JP2009175108A (ja) * | 2008-01-28 | 2009-08-06 | Sharp Corp | 分析用マイクロ流路デバイス |
JP2009180513A (ja) * | 2008-01-29 | 2009-08-13 | Fujitsu Ltd | ターゲット評価方法および装置 |
EP2158968A1 (en) | 2008-08-27 | 2010-03-03 | Sharp Kabushiki Kaisha | Detection instrument, analysis device, and detection method |
WO2021068912A1 (zh) * | 2019-10-11 | 2021-04-15 | 深圳华迈兴微医疗科技有限公司 | 一种多标志物检测的磁微粒发光微流控芯片以及检测装置 |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0522461D0 (en) | 2005-11-03 | 2005-12-14 | Immunoclin Ltd | Dactyl detector |
DE502007005679D1 (de) * | 2007-08-03 | 2010-12-30 | Micronas Gmbh | Kit und Vorrichtung zum Erzeugen einer Chemolumineszenzstrahlung |
CN102762971B (zh) | 2009-11-27 | 2015-05-27 | 洛桑联邦理工学院 | 纳米流体生物传感器及其对于快速测量溶液中的生物分子相互作用的应用及方法 |
CN104730067B (zh) * | 2015-03-16 | 2017-08-22 | 南京工业大学 | 一种磷脂的电致化学发光检测方法及其应用 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6454945B1 (en) * | 1995-06-16 | 2002-09-24 | University Of Washington | Microfabricated devices and methods |
CN1173776C (zh) * | 1996-06-28 | 2004-11-03 | 卡钳技术有限公司 | 在微规模流体性设备里的高通过量的筛选分析系统 |
JP3461696B2 (ja) | 1997-09-03 | 2003-10-27 | 日本電信電話株式会社 | 微少量オンラインバイオセンサー及びその製造方法 |
JP2001518624A (ja) | 1997-09-26 | 2001-10-16 | ユニバーシティ・オブ・ワシントン | 同時の粒子分離および化学反応 |
AU3498900A (en) * | 1999-02-23 | 2000-09-14 | Caliper Technologies Corporation | Sequencing by incorporation |
US6506609B1 (en) * | 1999-05-17 | 2003-01-14 | Caliper Technologies Corp. | Focusing of microparticles in microfluidic systems |
US6649358B1 (en) * | 1999-06-01 | 2003-11-18 | Caliper Technologies Corp. | Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities |
US6294392B1 (en) * | 1999-07-21 | 2001-09-25 | The Regents Of The University Of California | Spatially-encoded analyte detection |
JP3527185B2 (ja) | 2000-09-01 | 2004-05-17 | 日本電信電話株式会社 | 生体組織の電気化学計測方法 |
JP2002277478A (ja) | 2001-03-15 | 2002-09-25 | Kanagawa Acad Of Sci & Technol | 液液界面セグメントフロー方法とセグメント分析方法 |
-
2004
- 2004-01-23 JP JP2004015974A patent/JP3991034B2/ja not_active Expired - Fee Related
-
2005
- 2005-01-18 US US10/554,219 patent/US7863051B2/en not_active Expired - Fee Related
- 2005-01-18 WO PCT/JP2005/000811 patent/WO2005071393A2/en active Application Filing
- 2005-01-18 EP EP05709265A patent/EP1711827B1/en not_active Expired - Fee Related
- 2005-01-18 DE DE602005012674T patent/DE602005012674D1/de active Active
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007315753A (ja) * | 2006-05-23 | 2007-12-06 | Univ Of Electro-Communications | マイクロ化学チップ装置 |
JP2009175108A (ja) * | 2008-01-28 | 2009-08-06 | Sharp Corp | 分析用マイクロ流路デバイス |
JP2009180513A (ja) * | 2008-01-29 | 2009-08-13 | Fujitsu Ltd | ターゲット評価方法および装置 |
EP2158968A1 (en) | 2008-08-27 | 2010-03-03 | Sharp Kabushiki Kaisha | Detection instrument, analysis device, and detection method |
WO2021068912A1 (zh) * | 2019-10-11 | 2021-04-15 | 深圳华迈兴微医疗科技有限公司 | 一种多标志物检测的磁微粒发光微流控芯片以及检测装置 |
Also Published As
Publication number | Publication date |
---|---|
JP3991034B2 (ja) | 2007-10-17 |
US20060272433A1 (en) | 2006-12-07 |
EP1711827A2 (en) | 2006-10-18 |
WO2005071393A3 (en) | 2005-10-27 |
DE602005012674D1 (de) | 2009-03-26 |
US7863051B2 (en) | 2011-01-04 |
EP1711827B1 (en) | 2009-02-11 |
WO2005071393A2 (en) | 2005-08-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Yang et al. | based microfluidic devices: Emerging themes and applications | |
US7863051B2 (en) | Detecting element and detection method | |
KR100635110B1 (ko) | 현장분석용 랩온어칩 및 랩온어칩용 신호탐지기 | |
Lin et al. | Urine analysis in microfluidic devices | |
US20090042319A1 (en) | Biosensor Detection By Means Of Droplet Driving, Agitation, and Evaporation | |
EP3385713B1 (en) | Lateral flow assay device | |
Henares et al. | Integration of multianalyte sensing functions on a capillary-assembled microchip: Simultaneous determination of ion concentrations and enzymatic activities by a “drop-and-sip” technique | |
JP2006516721A (ja) | 多孔質層上に試薬を含む複層化された電気化学系微小流体センサー | |
KR101162817B1 (ko) | 마이크로 분석 측정 장치 및 이를 이용한 마이크로 분석 측정 방법 | |
DK1644737T3 (da) | Indretning og fremgangsmåde til simultan udförelse af blodgruppebestemmelse, serum-kontrapröve og anti-stofsögningstest | |
US20170010259A1 (en) | Multiplexed microfluidic proteomic platform | |
JP2009501908A (ja) | マイクロ流体装置およびその作製方法ならびに利用方法 | |
Guihen | Recent advances in miniaturization—The role of microchip electrophoresis in clinical analysis | |
JP6199527B1 (ja) | 濾過流制御を有する側方流アッセイ装置 | |
EP3433605A1 (en) | Surface enhanced raman spectroscopy (sers) microfluidics biosensor for detecting single and/or multiple analytes | |
JP2010099061A (ja) | 検出用カートリッジ及び被検出物質の検出方法 | |
Lee et al. | A whole blood sample-to-answer polymer lab-on-a-chip with superhydrophilic surface toward point-of-care technology | |
JP5139581B2 (ja) | 分析用ストリップ | |
Lin et al. | An integrated microfluidic system for the determination of microalbuminuria by measuring the albumin-to-creatinine ratio | |
Frey et al. | Continuous-flow multi-analyte biosensor cartridge with controllable linear response range | |
JP5522515B2 (ja) | 電気化学分析チップ | |
KR101894100B1 (ko) | 바이오 센서 | |
US20200292459A1 (en) | Metabolite detection apparatus and method of detecting metabolites | |
CA2932577C (en) | Gas evacuation system for nanofluidic biosensor | |
Dincer | Electrochemical microfluidic multiplexed biosensor platform for point-of-care testing |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20051125 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20070411 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20070418 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070615 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20070712 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20070723 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100727 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100727 Year of fee payment: 3 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110727 Year of fee payment: 4 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120727 Year of fee payment: 5 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120727 Year of fee payment: 5 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130727 Year of fee payment: 6 |
|
LAPS | Cancellation because of no payment of annual fees |