JP2004532033A5 - - Google Patents
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- Publication number
- JP2004532033A5 JP2004532033A5 JP2002587558A JP2002587558A JP2004532033A5 JP 2004532033 A5 JP2004532033 A5 JP 2004532033A5 JP 2002587558 A JP2002587558 A JP 2002587558A JP 2002587558 A JP2002587558 A JP 2002587558A JP 2004532033 A5 JP2004532033 A5 JP 2004532033A5
- Authority
- JP
- Japan
- Prior art keywords
- recombinant
- polyamino acid
- sumo
- ulp1
- protease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002253 acid Substances 0.000 claims description 23
- 108010018628 Ulp1 protease Proteins 0.000 claims description 17
- 102000037865 fusion proteins Human genes 0.000 claims description 16
- 108020001507 fusion proteins Proteins 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 15
- 239000012634 fragment Substances 0.000 claims description 11
- 239000013598 vector Substances 0.000 claims description 11
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 10
- 238000000746 purification Methods 0.000 claims description 10
- 238000003259 recombinant expression Methods 0.000 claims description 10
- 230000002708 enhancing effect Effects 0.000 claims description 4
- 229920001308 poly(aminoacid) Polymers 0.000 claims description 3
- 101000684503 Homo sapiens Sentrin-specific protease 3 Proteins 0.000 claims 6
- 102100023645 Sentrin-specific protease 3 Human genes 0.000 claims 6
- 102000044159 Ubiquitin Human genes 0.000 claims 4
- 108090000848 Ubiquitin Proteins 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 108091005804 Peptidases Proteins 0.000 claims 3
- 239000004365 Protease Substances 0.000 claims 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 3
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 3
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 claims 3
- 102000051619 SUMO-1 Human genes 0.000 claims 2
- 108700038981 SUMO-1 Proteins 0.000 claims 2
- 238000010367 cloning Methods 0.000 claims 2
- 101710180012 Protease 7 Proteins 0.000 claims 1
- 102000002933 Thioredoxin Human genes 0.000 claims 1
- 230000007547 defect Effects 0.000 claims 1
- 229910021645 metal ion Inorganic materials 0.000 claims 1
- 150000007523 nucleic acids Chemical group 0.000 claims 1
- 229920002704 polyhistidine Polymers 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 108060008226 thioredoxin Proteins 0.000 claims 1
- 229940094937 thioredoxin Drugs 0.000 claims 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 125000000539 amino acid group Chemical group 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28865601P | 2001-05-04 | 2001-05-04 | |
| US32908001P | 2001-10-12 | 2001-10-12 | |
| PCT/US2002/014062 WO2002090495A2 (en) | 2001-05-04 | 2002-05-06 | Rapidly cleavable sumo fusion protein expression system for difficult to express proteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004532033A JP2004532033A (ja) | 2004-10-21 |
| JP2004532033A5 true JP2004532033A5 (enExample) | 2010-02-25 |
Family
ID=26965151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002587558A Pending JP2004532033A (ja) | 2001-05-04 | 2002-05-06 | 発現し難いタンパク質用の迅速に開裂可能なｓｕｍｏ融合タンパク質発現系 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US6872551B2 (enExample) |
| EP (1) | EP1392717B1 (enExample) |
| JP (1) | JP2004532033A (enExample) |
| AT (1) | ATE490968T1 (enExample) |
| AU (1) | AU2002257243A1 (enExample) |
| CA (1) | CA2445049A1 (enExample) |
| DE (1) | DE60238532D1 (enExample) |
| WO (1) | WO2002090495A2 (enExample) |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2387655A1 (en) * | 1999-07-31 | 2001-02-08 | Board Of Regents, The University Of Texas System | Sentrin-specific human proteases senp1-3 |
| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| AU2002257243A1 (en) * | 2001-05-04 | 2002-11-18 | Cornell Research Foundation, Inc. | Rapidly cleavable sumo fusion protein expression system for difficult to express proteins |
| US8535908B2 (en) * | 2002-01-04 | 2013-09-17 | Brookhaven Science Associates, Llc | Facilitating protein solubility by use of peptide extensions |
| DE60318014T2 (de) * | 2002-01-07 | 2008-11-27 | Lifesensors, Inc. | Verfahren und zusammensetzungen für die proteinexpression und reinigung |
| US7220576B2 (en) * | 2002-01-07 | 2007-05-22 | Lifesensors, Inc. | Methods and compositions for protein expression and purification |
| US20040219516A1 (en) * | 2002-07-18 | 2004-11-04 | Invitrogen Corporation | Viral vectors containing recombination sites |
| WO2004063214A2 (en) * | 2003-01-08 | 2004-07-29 | Government Of The United States Of America As Represented By The Sercretary Of The Department Of Health And Human Services National Institutes Of Health | Antibodies that specifically recognize sumo-conjugated proteins |
| AU2004254600A1 (en) * | 2003-06-26 | 2005-01-13 | Lifesensors, Inc. | Methods and compositions for enhanced protein expression and purification |
| US20050069929A1 (en) | 2003-08-08 | 2005-03-31 | Invitrogen Corporation | Methods and compositions for seamless cloning of nucleic acid molecules |
| US8507277B2 (en) | 2003-10-24 | 2013-08-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides |
| US8062891B2 (en) | 2003-10-24 | 2011-11-22 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to plants |
| US8133733B2 (en) | 2003-10-24 | 2012-03-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to target tissues |
| US20090208478A1 (en) * | 2003-10-24 | 2009-08-20 | Gencia Corporation | Transducible polypeptides for modifying metabolism |
| US20090123468A1 (en) | 2003-10-24 | 2009-05-14 | Gencia Corporation | Transducible polypeptides for modifying metabolism |
| AU2004297533B2 (en) | 2003-10-24 | 2010-04-29 | Gencia Corporation | Methods and compositions for delivering polynucleotides |
| DE602004027538D1 (de) | 2003-12-01 | 2010-07-15 | Life Technologies Corp | Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon |
| ES2306541B1 (es) * | 2004-04-16 | 2009-09-28 | Consejo Superior Investig. Cientificas | Trasplante funcional de la maquinaria de sumoilacion a escherichia coli. |
| JP2008509682A (ja) * | 2004-08-18 | 2008-04-03 | ガヴァナーズ オブ ザ ユニヴァーシティー オブ アルバータ | Ｙｅｂｆを利用するタンパク質の製造方法 |
| EP1836214A4 (en) * | 2004-12-30 | 2009-01-21 | Lifesensors Inc | COMPOSITIONS, METHODS, AND KITS FOR IMPROVING PROTEIN EXPRESSION, SOLUBILITY, AND ISOLATION |
| CA2670014A1 (en) * | 2006-11-22 | 2008-11-20 | Emory University | Production of anti-microbial peptides |
| EP2100961A1 (en) * | 2008-03-04 | 2009-09-16 | Icon Genetics GmbH | Method of protease production in plants |
| TW200948959A (en) * | 2008-05-06 | 2009-12-01 | Academia Sinica | Preparation of soluble capsid proteins of picornavirus using SUMO fusion technology |
| TW200951219A (en) * | 2008-05-06 | 2009-12-16 | Academia Sinica | An improved SUMO fusion protein system for effective production of native proteins |
| US8440449B2 (en) * | 2008-09-30 | 2013-05-14 | The United States Of America, As Represented By The Secretary Of Agriculture | Transformed Saccharomyces cerevisiae engineered for xylose utilization |
| US8663951B2 (en) * | 2009-01-09 | 2014-03-04 | Academia Sinica | Method of producing virus-like particles of picornavirus using a small-ubiquitin-related fusion protein expression system |
| CN102533712A (zh) * | 2009-05-12 | 2012-07-04 | 中国科学院上海生命科学研究院 | 一种固相化sumo化系统及固相化去sumo化系统 |
| CN101993495B (zh) * | 2009-08-12 | 2013-07-24 | 上海近岸科技有限公司 | 一种蛋白质混合物及其制备方法 |
| CN103184228A (zh) * | 2011-12-29 | 2013-07-03 | 天津市国际生物医药联合研究院 | 用于可结晶成熟型SARS冠状病毒非结构蛋白12(sars-nsp12)的外源重组表达及纯化方法 |
| WO2013103964A1 (en) | 2012-01-06 | 2013-07-11 | Gencia Corporation | Methods and compositions for treating proteinopathies |
| WO2013188874A1 (en) | 2012-06-15 | 2013-12-19 | Gencia Corporation | Methods of mitigating side effects of radiation exposure and chemotherapy |
| WO2013188873A1 (en) | 2012-06-15 | 2013-12-19 | Gencia Corporation | Compositions and methods for enhancing immune responses |
| US10047352B2 (en) | 2012-10-03 | 2018-08-14 | Agrivida, Inc. | Intein-modified proteases, their production and industrial applications |
| KR101911438B1 (ko) | 2012-10-31 | 2018-10-24 | 삼성전자주식회사 | 이중 특이 항원 결합 단백질 복합체 및 이중 특이 항체의 제조 방법 |
| CN105377265A (zh) | 2013-03-15 | 2016-03-02 | 吉奇亚公司 | 用于治疗影响表皮的病状的组合物和方法 |
| WO2014197905A1 (en) | 2013-06-07 | 2014-12-11 | Georgia State University Research Foundation, Inc. | Compositions, methods, and assays for analyzing the structure, function, and activity of membrane proteins |
| US9447157B2 (en) | 2013-09-13 | 2016-09-20 | Augusta University Research Institute, Inc. | Nitration shielding peptides and methods of use thereof |
| WO2015049230A1 (en) | 2013-10-01 | 2015-04-09 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | An orthogonal set of tag-cleaving proteases for purification of proteins and stoichiometric protein complexes |
| EP3218472B1 (en) | 2014-11-10 | 2020-07-08 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Tag removal from proteins expressed in pro- and eukaryotic hosts |
| ES2862412T3 (es) * | 2015-10-14 | 2021-10-07 | Translate Bio Inc | Modificación de enzimas relacionados con el ARN para producción mejorada |
| TWI591177B (zh) * | 2016-10-27 | 2017-07-11 | 中化合成生技股份有限公司 | 一種胰高血糖素胜肽－２（ｇｌｐ－２）類似物的製備方法 |
| US10913777B2 (en) | 2017-05-22 | 2021-02-09 | The Regents Of The University Of California | In vitro assembly of bacterial microcompartments |
| JP7795181B2 (ja) | 2017-09-06 | 2026-01-07 | イェール ユニバーシティー | インターロイキン－１８バリアントとその利用法 |
| KR20190135393A (ko) * | 2018-05-28 | 2019-12-06 | 주식회사 원진바이오테크놀로지 | 폴리유비퀴틴 스캐폴드에 결합된 생체분자들의 선형 멀티머 중합체 및 이의 용도 |
| CN109517074A (zh) * | 2018-12-04 | 2019-03-26 | 湖北大学 | 一种融合标签促进Kuma030蛋白酶可溶性表达及非亲和层析快速纯化的应用 |
| CN109628479A (zh) * | 2018-12-25 | 2019-04-16 | 天津农学院 | 一种胰高血糖素样肽-1的重组表达方法 |
| CA3125556A1 (en) * | 2019-01-04 | 2020-07-09 | Repertoire Immune Medicines, Inc. | Methods of producing high diversity peptide libraries and promoting protein folding |
| JP2023522534A (ja) | 2020-02-03 | 2023-05-31 | プレマス バイオテック プライベート リミテッド | 発現が困難なタンパク質を発現させるための、組換え発現プラットフォーム、構築物、および方法 |
| CN113025675B (zh) * | 2021-05-21 | 2021-08-20 | 凯莱英医药集团（天津）股份有限公司 | 多肽的制备方法 |
| AU2023219791A1 (en) | 2022-02-14 | 2024-08-22 | University Of Georgia Research Foundation, Inc. | Pan-pneumovirus vaccine compositions and methods of use thereof. |
| CN114426586A (zh) * | 2022-02-21 | 2022-05-03 | 中国农业大学 | 一种胞外分泌表达Ulp1蛋白酶的大肠杆菌基因工程菌株及其应用 |
| CN114539425B (zh) * | 2022-02-25 | 2023-04-28 | 湖南中晟全肽生化有限公司 | 一种提高线性多肽生物表达的方法 |
| WO2026107236A1 (en) | 2024-11-13 | 2026-05-21 | University Of Georgia Research Foundation, Inc. | Compositions and methods for vaccination against mycobacterium tuberculosis |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5132213A (en) * | 1986-10-02 | 1992-07-21 | Massachusetts Institute Of Technology | Method for producing proteins and polypeptides using ubiquitin fusions |
| US5643758A (en) * | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
| WO1997001627A1 (en) * | 1995-06-27 | 1997-01-16 | Igen International, Inc. | High-level expression and efficient recovery of ubiquitin fusion proteins from escherichia coli |
| AU2002257243A1 (en) * | 2001-05-04 | 2002-11-18 | Cornell Research Foundation, Inc. | Rapidly cleavable sumo fusion protein expression system for difficult to express proteins |
| DE60318014T2 (de) * | 2002-01-07 | 2008-11-27 | Lifesensors, Inc. | Verfahren und zusammensetzungen für die proteinexpression und reinigung |
-
2002
- 2002-05-06 AU AU2002257243A patent/AU2002257243A1/en not_active Abandoned
- 2002-05-06 EP EP02726837A patent/EP1392717B1/en not_active Expired - Lifetime
- 2002-05-06 WO PCT/US2002/014062 patent/WO2002090495A2/en not_active Ceased
- 2002-05-06 AT AT02726837T patent/ATE490968T1/de not_active IP Right Cessation
- 2002-05-06 DE DE60238532T patent/DE60238532D1/de not_active Expired - Lifetime
- 2002-05-06 CA CA002445049A patent/CA2445049A1/en not_active Abandoned
- 2002-05-06 JP JP2002587558A patent/JP2004532033A/ja active Pending
- 2002-07-02 US US10/188,343 patent/US6872551B2/en not_active Expired - Lifetime
-
2004
- 2004-09-24 US US10/949,685 patent/US7498165B2/en not_active Expired - Fee Related
-
2008
- 2008-10-10 US US12/249,334 patent/US7910364B2/en not_active Expired - Lifetime
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