DE60238532D1 - Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine - Google Patents

Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine

Info

Publication number
DE60238532D1
DE60238532D1 DE60238532T DE60238532T DE60238532D1 DE 60238532 D1 DE60238532 D1 DE 60238532D1 DE 60238532 T DE60238532 T DE 60238532T DE 60238532 T DE60238532 T DE 60238532T DE 60238532 D1 DE60238532 D1 DE 60238532D1
Authority
DE
Germany
Prior art keywords
amino acid
expression system
sumofusion
cleavable
heavy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60238532T
Other languages
German (de)
English (en)
Inventor
Christopher D Lima
Elena Mossessova
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cornell Research Foundation Inc
Original Assignee
Cornell Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Research Foundation Inc filed Critical Cornell Research Foundation Inc
Application granted granted Critical
Publication of DE60238532D1 publication Critical patent/DE60238532D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60238532T 2001-05-04 2002-05-06 Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine Expired - Lifetime DE60238532D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28865601P 2001-05-04 2001-05-04
US32908001P 2001-10-12 2001-10-12
PCT/US2002/014062 WO2002090495A2 (en) 2001-05-04 2002-05-06 Rapidly cleavable sumo fusion protein expression system for difficult to express proteins

Publications (1)

Publication Number Publication Date
DE60238532D1 true DE60238532D1 (de) 2011-01-20

Family

ID=26965151

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60238532T Expired - Lifetime DE60238532D1 (de) 2001-05-04 2002-05-06 Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine

Country Status (8)

Country Link
US (3) US6872551B2 (enExample)
EP (1) EP1392717B1 (enExample)
JP (1) JP2004532033A (enExample)
AT (1) ATE490968T1 (enExample)
AU (1) AU2002257243A1 (enExample)
CA (1) CA2445049A1 (enExample)
DE (1) DE60238532D1 (enExample)
WO (1) WO2002090495A2 (enExample)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003506032A (ja) * 1999-07-31 2003-02-18 ボード オブ リージェンツ， ザ ユニバーシティ オブ テキサス システム セントリン特異的ヒトプロテアーゼｓｅｎｐ１−３
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DE60238532D1 (de) * 2001-05-04 2011-01-20 Cornell Res Foundation Inc Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
US8535908B2 (en) * 2002-01-04 2013-09-17 Brookhaven Science Associates, Llc Facilitating protein solubility by use of peptide extensions
US7060461B2 (en) 2002-01-07 2006-06-13 Lifesensors, Inc. Methods and compositions for protein expression and purification
US7220576B2 (en) * 2002-01-07 2007-05-22 Lifesensors, Inc. Methods and compositions for protein expression and purification
WO2004009768A2 (en) * 2002-07-18 2004-01-29 Invitrogen Corporation Viral vectors containing recombination sites
WO2004063214A2 (en) * 2003-01-08 2004-07-29 Government Of The United States Of America As Represented By The Sercretary Of The Department Of Health And Human Services National Institutes Of Health Antibodies that specifically recognize sumo-conjugated proteins
AU2004254600A1 (en) * 2003-06-26 2005-01-13 Lifesensors, Inc. Methods and compositions for enhanced protein expression and purification
EP1687323A4 (en) 2003-08-08 2009-08-12 Life Technologies Corp METHODS AND COMPOSITIONS FOR DIRECT CLONING OF NUCLEIC ACID MOLECULES
US8507277B2 (en) 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
JP4838722B2 (ja) 2003-10-24 2011-12-14 ゲンシア コーポレーション ポリヌクレオチドを送達する方法、及び送達用組成物
US8062891B2 (en) 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
US20090208478A1 (en) * 2003-10-24 2009-08-20 Gencia Corporation Transducible polypeptides for modifying metabolism
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
JP2007512838A (ja) 2003-12-01 2007-05-24 インヴィトロジェン コーポレーション 組換え部位を含む核酸分子およびその使用方法
ES2306541B1 (es) * 2004-04-16 2009-09-28 Consejo Superior Investig. Cientificas Trasplante funcional de la maquinaria de sumoilacion a escherichia coli.
CA2576083A1 (en) * 2004-08-18 2006-02-23 Joel Weiner Protein production method utilizing yebf
EP1836214A4 (en) * 2004-12-30 2009-01-21 Lifesensors Inc COMPOSITIONS, METHODS, AND KITS FOR IMPROVING PROTEIN EXPRESSION, SOLUBILITY, AND ISOLATION
CA2670014A1 (en) * 2006-11-22 2008-11-20 Emory University Production of anti-microbial peptides
EP2100961A1 (en) * 2008-03-04 2009-09-16 Icon Genetics GmbH Method of protease production in plants
US8034910B2 (en) * 2008-05-06 2011-10-11 Academia Sinica, Taiwan SUMO fusion protein expression system for producing native proteins
US20090280531A1 (en) * 2008-05-06 2009-11-12 Academia Sinica Preparation of Soluble Capsid Proteins of Picornaviruses Using SUMO Fusion Technology
US8440449B2 (en) * 2008-09-30 2013-05-14 The United States Of America, As Represented By The Secretary Of Agriculture Transformed Saccharomyces cerevisiae engineered for xylose utilization
US8663951B2 (en) * 2009-01-09 2014-03-04 Academia Sinica Method of producing virus-like particles of picornavirus using a small-ubiquitin-related fusion protein expression system
CN102533712A (zh) * 2009-05-12 2012-07-04 中国科学院上海生命科学研究院 一种固相化sumo化系统及固相化去sumo化系统
CN101993495B (zh) * 2009-08-12 2013-07-24 上海近岸科技有限公司 一种蛋白质混合物及其制备方法
CN103184228A (zh) * 2011-12-29 2013-07-03 天津市国际生物医药联合研究院 用于可结晶成熟型SARS冠状病毒非结构蛋白12(sars-nsp12)的外源重组表达及纯化方法
WO2013103972A1 (en) 2012-01-06 2013-07-11 Gencia Corporation Treatment of frailty syndrome and symptoms thereof
US20150283233A1 (en) 2012-06-15 2015-10-08 Gencia Corporation Compositions and Methods for Enhancing Immune Responses
WO2013188874A1 (en) 2012-06-15 2013-12-19 Gencia Corporation Methods of mitigating side effects of radiation exposure and chemotherapy
WO2014055782A1 (en) 2012-10-03 2014-04-10 Agrivida, Inc. Intein-modified proteases, their production and industrial applications
KR101911438B1 (ko) * 2012-10-31 2018-10-24 삼성전자주식회사 이중 특이 항원 결합 단백질 복합체 및 이중 특이 항체의 제조 방법
RU2015143130A (ru) 2013-03-15 2017-04-21 Дженсиа Корпорейшен Композиции и способы лечения состояний, поражающих эпидермис
US9970945B2 (en) 2013-06-07 2018-05-15 Georgia State University Research Foundtion, Inc. Compositions, methods, and assays using proteo-liposome-transfected oocytes for analyzing the structure, function, and activity of membrane proteins
US9447157B2 (en) 2013-09-13 2016-09-20 Augusta University Research Institute, Inc. Nitration shielding peptides and methods of use thereof
WO2015049230A1 (en) 2013-10-01 2015-04-09 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. An orthogonal set of tag-cleaving proteases for purification of proteins and stoichiometric protein complexes
EP3218472B1 (en) 2014-11-10 2020-07-08 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Tag removal from proteins expressed in pro- and eukaryotic hosts
CA3001852A1 (en) * 2015-10-14 2017-04-20 Translate Bio, Inc. Modification of rna-related enzymes for enhanced production
TWI591177B (zh) * 2016-10-27 2017-07-11 中化合成生技股份有限公司 一種胰高血糖素胜肽－２（ｇｌｐ－２）類似物的製備方法
US10913777B2 (en) 2017-05-22 2021-02-09 The Regents Of The University Of California In vitro assembly of bacterial microcompartments
WO2019051015A1 (en) 2017-09-06 2019-03-14 Yale University VARIANTS OF INTERLEUKIN-18 AND METHODS OF USE
KR20190135393A (ko) * 2018-05-28 2019-12-06 주식회사 원진바이오테크놀로지 폴리유비퀴틴 스캐폴드에 결합된 생체분자들의 선형 멀티머 중합체 및 이의 용도
CN109517074A (zh) * 2018-12-04 2019-03-26 湖北大学 一种融合标签促进Kuma030蛋白酶可溶性表达及非亲和层析快速纯化的应用
CN109628479A (zh) * 2018-12-25 2019-04-16 天津农学院 一种胰高血糖素样肽-1的重组表达方法
WO2020142720A1 (en) * 2019-01-04 2020-07-09 Cogen Immune Medicine, Inc. Methods of producing high diversity peptide libraries and promoting protein folding
CN113025675B (zh) * 2021-05-21 2021-08-20 凯莱英医药集团（天津）股份有限公司 多肽的制备方法
EP4479088A1 (en) 2022-02-14 2024-12-25 University of Georgia Research Foundation, Inc. Pan-pneumovirus vaccine compositions and methods of use thereof
CN114426586A (zh) * 2022-02-21 2022-05-03 中国农业大学 一种胞外分泌表达Ulp1蛋白酶的大肠杆菌基因工程菌株及其应用
CN114539425B (zh) * 2022-02-25 2023-04-28 湖南中晟全肽生化有限公司 一种提高线性多肽生物表达的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5132213A (en) * 1986-10-02 1992-07-21 Massachusetts Institute Of Technology Method for producing proteins and polypeptides using ubiquitin fusions
US5643758A (en) * 1987-03-10 1997-07-01 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
WO1997001627A1 (en) * 1995-06-27 1997-01-16 Igen International, Inc. High-level expression and efficient recovery of ubiquitin fusion proteins from escherichia coli
DE60238532D1 (de) * 2001-05-04 2011-01-20 Cornell Res Foundation Inc Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
US7060461B2 (en) 2002-01-07 2006-06-13 Lifesensors, Inc. Methods and compositions for protein expression and purification

Also Published As

Publication number Publication date
US20050069988A1 (en) 2005-03-31
AU2002257243A1 (en) 2002-11-18
WO2002090495A2 (en) 2002-11-14
US20030086918A1 (en) 2003-05-08
US20090317868A1 (en) 2009-12-24
EP1392717A1 (en) 2004-03-03
EP1392717A4 (en) 2005-06-08
US7498165B2 (en) 2009-03-03
WO2002090495A8 (en) 2003-07-03
US6872551B2 (en) 2005-03-29
CA2445049A1 (en) 2002-11-14
ATE490968T1 (de) 2010-12-15
JP2004532033A (ja) 2004-10-21
EP1392717B1 (en) 2010-12-08
US7910364B2 (en) 2011-03-22

Similar Documents

Publication Publication Date Title
DE60238532D1 (de) Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
Backes et al. An alkanesulfonamide “safety-catch” linker for solid-phase synthesis
CA2443365A1 (en) Methods for the recombinant production of antifusogenic peptides
Brik et al. Protein synthesis by solid-phase chemical ligation using a safety catch linker
CO6251278A2 (es) Metodo para la produccion de conjugados del factor de crecimiento similar a la insulina -1 y polietilenglicol
WO2004022697A3 (en) Denaturat stable and/or protease resistant, chaperone-like oligomeric proteins, polynucleotides encoding same, their uses and methods of increasing a specific activity thereof
DE60021318D1 (de) Verfahren zur Herstellung von natürlich gefalteten Proteinen durch Co-Sekretion von molekularen Chaperonen
SI1874932T1 (sl) Proizvodnja rekombinantnih proteinov z avtoproteolitično cepitvijo fuzijskega proteina
RU2008137800A (ru) Получение биологически активных белков
AU2002234863A1 (en) Denaturat stable and/or protease resistant, chaperone-like oligomeric proteins, polynucleotides encoding same and their uses
ATE121454T1 (de) Ubiquitinspezifische protease.
ZA200305869B (en) Fusion protein for the secretion of a protein of interest into the supernatant of the bacterial culture.
Berkers et al. Transpeptidation and reverse proteolysis and their consequences for immunity
ATE236259T1 (de) Herstellung einzelner genprodukte eines chimären fusionsproteins durch spaltung mit überall vorkommenden endoproteasen
MXPA05012336A (es) Procedimiento para producir una proteina dirigida a un plastidio en celulas vegetales.
CA2379571A1 (en) Process for the production of a heterologous peptide by autoproteolytic cleavage in vitro
PE20020875A1 (es) Peptidos supersegregables en procedimientos para su produccion y para la mejora en paralelo de las formas exportadas de uno o mas de otros peptidos de interes
EA200101161A1 (ru) Способ получения рекомбинантного инсулина из новых гибридных белков
MXPA03011453A (es) Acetilacion de fragmentos de gp41.
Schafer et al. A highly specific system for efficient enzymatic removal of tags from recombinant proteins
JPH01160496A (ja) 融合タンパク質の選択的分解方法
Likhareva et al. Synthetic model and bioactive peptides as potential substrates for enteropeptidase
US20110124841A1 (en) Polypeptides Modified by Protein Trans-Splicing Technology
ATE309263T1 (de) Reinigungsverfahren auf grundlage der bindung von enzymen and markierte peptide
Nguyen et al. Numaswitch–The First Viable Alternative for Chemical Synthesis of Peptides and Pepteins