JP2003526364A5

JP2003526364A5 -

Download PDF

Info

Publication number: JP2003526364A5
Authority: JP; Japan
Prior art keywords: luciferase; gfp; renilla; protein; bioluminescence
Prior art date: 2001-03-15
Legal status : Granted

Application number

JP2001567308A

Other languages

English (en)

Japanese (ja)

Other versions

JP4830063B2 (ja

JP2003526364A (ja

Filing date

2001-03-15

Publication date

2008-05-01

2001-03-15 Application filed filed Critical

2001-03-15 Priority claimed from PCT/US2001/008277 external-priority patent/WO2001068824A2/en

2003-09-09 Publication of JP2003526364A publication Critical patent/JP2003526364A/ja

2008-05-01 Publication of JP2003526364A5 publication Critical patent/JP2003526364A5/ja

2011-12-07 Application granted granted Critical

2011-12-07 Publication of JP4830063B2 publication Critical patent/JP4830063B2/ja

2021-03-15 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

108060001084 Luciferase family Proteins 0.000 description 481
239000005089 Luciferase Substances 0.000 description 430
239000005090 green fluorescent protein Substances 0.000 description 417
108010043121 Green Fluorescent Proteins Proteins 0.000 description 399
102000004144 Green Fluorescent Proteins Human genes 0.000 description 399
239000000203 mixture Substances 0.000 description 239
238000005415 bioluminescence Methods 0.000 description 185
230000029918 bioluminescence Effects 0.000 description 166
241000242739 Renilla Species 0.000 description 154
238000006243 chemical reaction Methods 0.000 description 154
IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 143
241000242743 Renilla reniformis Species 0.000 description 143
210000004027 cells Anatomy 0.000 description 132
102000004169 proteins and genes Human genes 0.000 description 132
108090000623 proteins and genes Proteins 0.000 description 131
235000018102 proteins Nutrition 0.000 description 128
229920003013 deoxyribonucleic acid Polymers 0.000 description 126
239000000306 component Substances 0.000 description 111
108020004707 nucleic acids Proteins 0.000 description 109
150000007523 nucleic acids Chemical class 0.000 description 108
MWUXSHHQAYIFBG-UHFFFAOYSA-N nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 102
239000000758 substrate Substances 0.000 description 78
239000003795 chemical substances by application Substances 0.000 description 70
210000001519 tissues Anatomy 0.000 description 69
230000027455 binding Effects 0.000 description 67
108090001123 antibodies Proteins 0.000 description 66
102000004965 antibodies Human genes 0.000 description 66
230000014509 gene expression Effects 0.000 description 53
235000001014 amino acid Nutrition 0.000 description 49
238000004166 bioassay Methods 0.000 description 49
241001521365 Renilla muelleri Species 0.000 description 47
108010000239 Aequorin Proteins 0.000 description 43
150000001875 compounds Chemical class 0.000 description 42
150000001413 amino acids Chemical class 0.000 description 39
239000003153 chemical reaction reagent Substances 0.000 description 39
241000963438 Gaussia <copepod> Species 0.000 description 38
-1 for example Chemical class 0.000 description 38
239000000126 substance Substances 0.000 description 38
229920002676 Complementary DNA Polymers 0.000 description 37
206010028980 Neoplasm Diseases 0.000 description 37
229920001184 polypeptide Polymers 0.000 description 37
239000002299 complementary DNA Substances 0.000 description 36
108020001507 fusion proteins Proteins 0.000 description 36
102000037240 fusion proteins Human genes 0.000 description 36
125000003729 nucleotide group Chemical group 0.000 description 36
MYMOFIZGZYHOMD-UHFFFAOYSA-N oxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 35
241000196324 Embryophyta Species 0.000 description 34
125000003275 alpha amino acid group Chemical group 0.000 description 32
239000000047 product Substances 0.000 description 32
229920001850 Nucleic acid sequence Polymers 0.000 description 31
230000000694 effects Effects 0.000 description 31
241000243290 Aequorea Species 0.000 description 30
241000894006 Bacteria Species 0.000 description 30
241000238584 Vargula Species 0.000 description 30
YHIPILPTUVMWQT-UHFFFAOYSA-N Coelenterazine Chemical compound C1=CC(O)=CC=C1CC(C(N1C=C(N2)C=3C=CC(O)=CC=3)=O)=NC1=C2CC1=CC=CC=C1 YHIPILPTUVMWQT-UHFFFAOYSA-N 0.000 description 29
102000034387 fluorescent proteins Human genes 0.000 description 28
108091006031 fluorescent proteins Proteins 0.000 description 28
239000003446 ligand Substances 0.000 description 28
239000002773 nucleotide Substances 0.000 description 28
102000005962 receptors Human genes 0.000 description 27
108020003175 receptors Proteins 0.000 description 27
230000001580 bacterial Effects 0.000 description 26
230000035492 administration Effects 0.000 description 25
239000000427 antigen Substances 0.000 description 25
239000001301 oxygen Substances 0.000 description 25
229910052760 oxygen Inorganic materials 0.000 description 25
229920002477 rna polymer Polymers 0.000 description 25
108060003162 GFP Proteins 0.000 description 24
241001465754 Metazoa Species 0.000 description 23
102000038129 antigens Human genes 0.000 description 23
108091007172 antigens Proteins 0.000 description 23
230000003993 interaction Effects 0.000 description 22
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 22
239000012190 activator Substances 0.000 description 21
238000004020 luminiscence type Methods 0.000 description 21
241000242764 Aequorea victoria Species 0.000 description 20
102000004190 Enzymes Human genes 0.000 description 20
108090000790 Enzymes Proteins 0.000 description 20
241000588724 Escherichia coli Species 0.000 description 20
238000004519 manufacturing process Methods 0.000 description 20
230000000051 modifying Effects 0.000 description 19
238000006467 substitution reaction Methods 0.000 description 19
241001343656 Ptilosarcus Species 0.000 description 18
238000007792 addition Methods 0.000 description 18
BHPQYMZQTOCNFJ-UHFFFAOYSA-N calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 18
239000000969 carrier Substances 0.000 description 18
230000003595 spectral Effects 0.000 description 18
229920002521 Macromolecule Polymers 0.000 description 17
241001343647 Pleuromamma Species 0.000 description 17
230000015572 biosynthetic process Effects 0.000 description 17
229910001424 calcium ion Inorganic materials 0.000 description 17
238000001514 detection method Methods 0.000 description 17
238000002866 fluorescence resonance energy transfer Methods 0.000 description 17
238000000338 in vitro Methods 0.000 description 17
238000007254 oxidation reaction Methods 0.000 description 17
238000002360 preparation method Methods 0.000 description 17
239000011780 sodium chloride Substances 0.000 description 17
241000894007 species Species 0.000 description 17
230000000699 topical Effects 0.000 description 17
241000238631 Hexapoda Species 0.000 description 16
238000010367 cloning Methods 0.000 description 16
229910001882 dioxygen Inorganic materials 0.000 description 16
239000003814 drug Substances 0.000 description 16
241000251468 Actinopterygii Species 0.000 description 15
201000010099 disease Diseases 0.000 description 15
239000003973 paint Substances 0.000 description 15
241000254158 Lampyridae Species 0.000 description 14
240000004808 Saccharomyces cerevisiae Species 0.000 description 14
235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 14
239000002253 acid Substances 0.000 description 14
239000011575 calcium Substances 0.000 description 14
235000013305 food Nutrition 0.000 description 14
238000002955 isolation Methods 0.000 description 14
230000003647 oxidation Effects 0.000 description 14
239000002904 solvent Substances 0.000 description 14
108010083590 Apoproteins Proteins 0.000 description 13
102000006410 Apoproteins Human genes 0.000 description 13
108090000363 Bacterial Luciferases Proteins 0.000 description 13
241000244206 Nematoda Species 0.000 description 13
238000011065 in-situ storage Methods 0.000 description 13
239000007788 liquid Substances 0.000 description 13
239000000463 material Substances 0.000 description 13
239000011159 matrix material Substances 0.000 description 13
238000000034 method Methods 0.000 description 13
238000001356 surgical procedure Methods 0.000 description 13
108091006028 chimera Proteins 0.000 description 12
229940079593 drugs Drugs 0.000 description 12
230000001613 neoplastic Effects 0.000 description 12
239000000843 powder Substances 0.000 description 12
239000000243 solution Substances 0.000 description 12
241000035538 Cypridina Species 0.000 description 11
230000005284 excitation Effects 0.000 description 11
230000004927 fusion Effects 0.000 description 11
150000002500 ions Chemical class 0.000 description 11
239000000523 sample Substances 0.000 description 11
239000000725 suspension Substances 0.000 description 11
229940088598 Enzyme Drugs 0.000 description 10
150000001299 aldehydes Chemical class 0.000 description 10
238000004458 analytical method Methods 0.000 description 10
108010041089 apoaequorin Proteins 0.000 description 10
238000003745 diagnosis Methods 0.000 description 10
238000002868 homogeneous time resolved fluorescence Methods 0.000 description 10
229910052751 metal Inorganic materials 0.000 description 10
239000002184 metal Substances 0.000 description 10
230000035772 mutation Effects 0.000 description 10
239000000376 reactant Substances 0.000 description 10
150000003839 salts Chemical class 0.000 description 10
238000001228 spectrum Methods 0.000 description 10
125000003396 thiol group Chemical group [H]S* 0.000 description 10
ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 9
108020004711 Nucleic Acid Probes Proteins 0.000 description 9
238000005755 formation reaction Methods 0.000 description 9
239000004615 ingredient Substances 0.000 description 9
239000002853 nucleic acid probe Substances 0.000 description 9
238000000746 purification Methods 0.000 description 9
238000003786 synthesis reaction Methods 0.000 description 9
239000000370 acceptor Substances 0.000 description 8
OYPRJOBELJOOCE-UHFFFAOYSA-N calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 8
229910052791 calcium Inorganic materials 0.000 description 8
239000002537 cosmetic Substances 0.000 description 8
238000011161 development Methods 0.000 description 8
230000005281 excited state Effects 0.000 description 8
238000002875 fluorescence polarization Methods 0.000 description 8
235000013350 formula milk Nutrition 0.000 description 8
239000000543 intermediate Substances 0.000 description 8
238000003530 single readout Methods 0.000 description 8
239000000344 soap Substances 0.000 description 8
FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
239000007921 spray Substances 0.000 description 8
230000002194 synthesizing Effects 0.000 description 8
230000035897 transcription Effects 0.000 description 8
DGVVWUTYPXICAM-UHFFFAOYSA-N 2-mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 7
241000238424 Crustacea Species 0.000 description 7
108090000331 Firefly luciferases Proteins 0.000 description 7
108010010803 Gelatin Proteins 0.000 description 7
239000000443 aerosol Substances 0.000 description 7
235000013361 beverage Nutrition 0.000 description 7
238000000225 bioluminescence resonance energy transfer Methods 0.000 description 7
238000007374 clinical diagnostic method Methods 0.000 description 7
239000006071 cream Substances 0.000 description 7
230000018109 developmental process Effects 0.000 description 7
201000009910 diseases by infectious agent Diseases 0.000 description 7
238000000295 emission spectrum Methods 0.000 description 7
FVTCRASFADXXNN-SCRDCRAPSA-N flavin mononucleotide Chemical group OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-SCRDCRAPSA-N 0.000 description 7
239000006260 foam Substances 0.000 description 7
239000000499 gel Substances 0.000 description 7
239000008273 gelatin Substances 0.000 description 7
229920000159 gelatin Polymers 0.000 description 7
235000019322 gelatine Nutrition 0.000 description 7
235000011852 gelatine desserts Nutrition 0.000 description 7
230000002458 infectious Effects 0.000 description 7
239000006210 lotion Substances 0.000 description 7
239000003550 marker Substances 0.000 description 7
108010045030 monoclonal antibodies Proteins 0.000 description 7
102000005614 monoclonal antibodies Human genes 0.000 description 7
238000002703 mutagenesis Methods 0.000 description 7
231100000350 mutagenesis Toxicity 0.000 description 7
239000006072 paste Substances 0.000 description 7
239000002157 polynucleotide Substances 0.000 description 7
229920000023 polynucleotide Polymers 0.000 description 7
238000003259 recombinant expression Methods 0.000 description 7
239000007787 solid Substances 0.000 description 7
239000000606 toothpaste Substances 0.000 description 7
229920001405 Coding region Polymers 0.000 description 6
241000700108 Ctenophora <comb jellyfish phylum> Species 0.000 description 6
229940013640 Flavin Mononucleotide Drugs 0.000 description 6
MTCFGRXMJLQNBG-REOHCLBHSA-N L-serine Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 6
241000237852 Mollusca Species 0.000 description 6
BAWFJGJZGIEFAR-NNYOXOHSSA-N Nicotinamide adenine dinucleotide Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 6
241001455233 Obelia Species 0.000 description 6
108090000417 Oxygenases Proteins 0.000 description 6
102000004020 Oxygenases Human genes 0.000 description 6
241000242583 Scyphozoa Species 0.000 description 6
238000010521 absorption reaction Methods 0.000 description 6
239000000654 additive Substances 0.000 description 6
125000000539 amino acid group Chemical group 0.000 description 6
239000011230 binding agent Substances 0.000 description 6
239000012472 biological sample Substances 0.000 description 6
238000011109 contamination Methods 0.000 description 6
230000000875 corresponding Effects 0.000 description 6
239000003937 drug carrier Substances 0.000 description 6
235000013601 eggs Nutrition 0.000 description 6
239000011768 flavin mononucleotide Substances 0.000 description 6
230000001965 increased Effects 0.000 description 6
238000001990 intravenous administration Methods 0.000 description 6
239000002609 media Substances 0.000 description 6
229920002106 messenger RNA Polymers 0.000 description 6
OZAIFHULBGXAKX-UHFFFAOYSA-N precursor Substances N#CC(C)(C)N=NC(C)(C)C#N OZAIFHULBGXAKX-UHFFFAOYSA-N 0.000 description 6
230000001105 regulatory Effects 0.000 description 6
229950001574 riboflavin phosphate Drugs 0.000 description 6
235000019231 riboflavin-5'-phosphate Nutrition 0.000 description 6
239000003981 vehicle Substances 0.000 description 6
241000282412 Homo Species 0.000 description 5
102000006830 Luminescent Proteins Human genes 0.000 description 5
108010047357 Luminescent Proteins Proteins 0.000 description 5
108020004511 Recombinant DNA Proteins 0.000 description 5
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 5
241000607618 Vibrio harveyi Species 0.000 description 5
150000007513 acids Chemical class 0.000 description 5
239000004480 active ingredient Substances 0.000 description 5
239000012491 analyte Substances 0.000 description 5
235000013405 beer Nutrition 0.000 description 5
UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 5
230000003197 catalytic Effects 0.000 description 5
230000001413 cellular Effects 0.000 description 5
235000019993 champagne Nutrition 0.000 description 5
235000019504 cigarettes Nutrition 0.000 description 5
239000003086 colorant Substances 0.000 description 5
230000000295 complement Effects 0.000 description 5
229920001577 copolymer Polymers 0.000 description 5
238000009472 formulation Methods 0.000 description 5
230000001939 inductive effect Effects 0.000 description 5
230000001404 mediated Effects 0.000 description 5
230000004048 modification Effects 0.000 description 5
238000006011 modification reaction Methods 0.000 description 5
229950006238 nadide Drugs 0.000 description 5
238000011160 research Methods 0.000 description 5
230000035945 sensitivity Effects 0.000 description 5
231100000486 side effect Toxicity 0.000 description 5
101710044770 sll1951 Proteins 0.000 description 5
230000002459 sustained Effects 0.000 description 5
238000006276 transfer reaction Methods 0.000 description 5
235000014101 wine Nutrition 0.000 description 5
241000243818 Annelida Species 0.000 description 4
241001331164 Aristostomias Species 0.000 description 4
108020004705 Codon Proteins 0.000 description 4
102000033147 ERVK-25 Human genes 0.000 description 4
YTNIXZGTHTVJBW-SCRDCRAPSA-N FMNH2 Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2NC2=C1NC(=O)NC2=O YTNIXZGTHTVJBW-SCRDCRAPSA-N 0.000 description 4
241000233866 Fungi Species 0.000 description 4
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 4
108020004999 Messenger RNA Proteins 0.000 description 4
102000003840 Opioid Receptors Human genes 0.000 description 4
108090000137 Opioid Receptors Proteins 0.000 description 4
241000233855 Orchidaceae Species 0.000 description 4
241000085362 Pachystomias Species 0.000 description 4
241000283984 Rodentia Species 0.000 description 4
229940034610 Toothpaste Drugs 0.000 description 4
102000004338 Transferrin Human genes 0.000 description 4
108090000901 Transferrin Proteins 0.000 description 4
230000003213 activating Effects 0.000 description 4
230000000996 additive Effects 0.000 description 4
230000000890 antigenic Effects 0.000 description 4
238000000423 cell based assay Methods 0.000 description 4
230000036978 cell physiology Effects 0.000 description 4
239000002738 chelating agent Substances 0.000 description 4
238000010276 construction Methods 0.000 description 4
239000000287 crude extract Substances 0.000 description 4
238000001212 derivatisation Methods 0.000 description 4
238000009826 distribution Methods 0.000 description 4
239000000428 dust Substances 0.000 description 4
230000002708 enhancing Effects 0.000 description 4
210000003527 eukaryotic cell Anatomy 0.000 description 4
239000000284 extract Substances 0.000 description 4
239000012530 fluid Substances 0.000 description 4
DHMQDGOQFOQNFH-UHFFFAOYSA-N glycine Chemical group NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
238000003018 immunoassay Methods 0.000 description 4
230000003834 intracellular Effects 0.000 description 4
108020001756 ligand binding domains Proteins 0.000 description 4
238000004377 microelectronic Methods 0.000 description 4
229920002401 polyacrylamide Polymers 0.000 description 4
229920001223 polyethylene glycol Polymers 0.000 description 4
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 4
102000004196 processed proteins & peptides Human genes 0.000 description 4
108090000765 processed proteins & peptides Proteins 0.000 description 4
108020001580 protein domains Proteins 0.000 description 4
238000006862 quantum yield reaction Methods 0.000 description 4
238000005215 recombination Methods 0.000 description 4
239000003638 reducing agent Substances 0.000 description 4
238000002165 resonance energy transfer Methods 0.000 description 4
230000004044 response Effects 0.000 description 4
125000003607 serino group Chemical group [H]OC(=O)C([H])(N([H])*)C([H])([H])O[H] 0.000 description 4
239000011734 sodium Substances 0.000 description 4
235000014214 soft drink Nutrition 0.000 description 4
239000012581 transferrin Substances 0.000 description 4
230000001960 triggered Effects 0.000 description 4
210000004881 tumor cells Anatomy 0.000 description 4
102100001249 ALB Human genes 0.000 description 3
UDMBCSSLTHHNCD-KQYNXXCUSA-N Adenosine monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 3
102100001766 BLVRB Human genes 0.000 description 3
101700034587 BLVRB Proteins 0.000 description 3
241000700668 Beroe ovata Species 0.000 description 3
210000000601 Blood Cells Anatomy 0.000 description 3
102000014914 Carrier Proteins Human genes 0.000 description 3
108010078791 Carrier Proteins Proteins 0.000 description 3
241000282693 Cercopithecidae Species 0.000 description 3
241000243321 Cnidaria Species 0.000 description 3
241000254173 Coleoptera Species 0.000 description 3
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 3
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 3
241000238557 Decapoda Species 0.000 description 3
101700000111 FRED Proteins 0.000 description 3
239000004471 Glycine Chemical group 0.000 description 3
241001275863 Gnathophausia Species 0.000 description 3
229940088597 Hormone Drugs 0.000 description 3
241000254054 Luciola cruciata Species 0.000 description 3
241000124008 Mammalia Species 0.000 description 3
241001126922 Mnemiopsis Species 0.000 description 3
210000000282 Nails Anatomy 0.000 description 3
241000426956 Pelagia Species 0.000 description 3
241000254064 Photinus pyralis Species 0.000 description 3
239000002202 Polyethylene glycol Substances 0.000 description 3
102000014961 Protein Precursors Human genes 0.000 description 3
108010078762 Protein Precursors Proteins 0.000 description 3
235000004789 Rosa xanthina Nutrition 0.000 description 3
241000109329 Rosa xanthina Species 0.000 description 3
235000003534 Saccharomyces carlsbergensis Nutrition 0.000 description 3
229940081969 Saccharomyces cerevisiae Drugs 0.000 description 3
210000003491 Skin Anatomy 0.000 description 3
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 3
UHUFTBALEZWWIH-UHFFFAOYSA-N Tetradecanal Natural products CCCCCCCCCCCCCC=O UHUFTBALEZWWIH-UHFFFAOYSA-N 0.000 description 3
241000607598 Vibrio Species 0.000 description 3
125000003277 amino group Chemical group 0.000 description 3
210000004102 animal cell Anatomy 0.000 description 3
230000000259 anti-tumor Effects 0.000 description 3
229960000070 antineoplastic Monoclonal antibodies Drugs 0.000 description 3
229910000266 aqualite Inorganic materials 0.000 description 3
WVDDGKGOMKODPV-UHFFFAOYSA-N benzyl alcohol Chemical compound OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 description 3
108091005941 blue fluorescent protein Proteins 0.000 description 3
238000010804 cDNA synthesis Methods 0.000 description 3
239000002775 capsule Substances 0.000 description 3
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
230000015556 catabolic process Effects 0.000 description 3
238000004587 chromatography analysis Methods 0.000 description 3
230000002759 chromosomal Effects 0.000 description 3
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
230000004059 degradation Effects 0.000 description 3
238000006731 degradation reaction Methods 0.000 description 3
230000001809 detectable Effects 0.000 description 3
238000002405 diagnostic procedure Methods 0.000 description 3
239000002552 dosage form Substances 0.000 description 3
KCXVZYZYPLLWCC-UHFFFAOYSA-N edta Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
238000000695 excitation spectrum Methods 0.000 description 3
239000011521 glass Substances 0.000 description 3
239000008187 granular material Substances 0.000 description 3
229910001385 heavy metal Inorganic materials 0.000 description 3
239000005556 hormone Substances 0.000 description 3
238000009396 hybridization Methods 0.000 description 3
230000001976 improved Effects 0.000 description 3
239000000976 ink Substances 0.000 description 3
238000003780 insertion Methods 0.000 description 3
239000002502 liposome Substances 0.000 description 3
239000006166 lysate Substances 0.000 description 3
235000013372 meat Nutrition 0.000 description 3
244000005700 microbiome Species 0.000 description 3
229960000060 monoclonal antibodies Drugs 0.000 description 3
230000003000 nontoxic Effects 0.000 description 3
231100000252 nontoxic Toxicity 0.000 description 3
QVGXLLKOCUKJST-UHFFFAOYSA-N oxygen atom Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
239000005022 packaging material Substances 0.000 description 3
238000004806 packaging method and process Methods 0.000 description 3
235000021317 phosphate Nutrition 0.000 description 3
239000002953 phosphate buffered saline Substances 0.000 description 3
230000002285 radioactive Effects 0.000 description 3
108010054624 red fluorescent protein Proteins 0.000 description 3
238000000926 separation method Methods 0.000 description 3
238000007920 subcutaneous administration Methods 0.000 description 3
235000000346 sugar Nutrition 0.000 description 3
239000003826 tablet Substances 0.000 description 3
239000004753 textile Substances 0.000 description 3
230000002588 toxic Effects 0.000 description 3
231100000331 toxic Toxicity 0.000 description 3
238000011144 upstream manufacturing Methods 0.000 description 3
LLXVXPPXELIDGQ-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 3-(2,5-dioxopyrrol-1-yl)benzoate Chemical compound C=1C=CC(N2C(C=CC2=O)=O)=CC=1C(=O)ON1C(=O)CCC1=O LLXVXPPXELIDGQ-UHFFFAOYSA-N 0.000 description 2
JWDFQMWEFLOOED-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 3-(pyridin-2-yldisulfanyl)propanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCSSC1=CC=CC=N1 JWDFQMWEFLOOED-UHFFFAOYSA-N 0.000 description 2
DIYPCWKHSODVAP-UHFFFAOYSA-N 1-[3-(2,5-dioxopyrrol-1-yl)benzoyl]oxy-2,5-dioxopyrrolidine-3-sulfonic acid Chemical compound O=C1C(S(=O)(=O)O)CC(=O)N1OC(=O)C1=CC=CC(N2C(C=CC2=O)=O)=C1 DIYPCWKHSODVAP-UHFFFAOYSA-N 0.000 description 2
YXOLAZRVSSWPPT-UHFFFAOYSA-N 3,5,7,2',4'-Pentahydroxyflavonol Chemical compound OC1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 YXOLAZRVSSWPPT-UHFFFAOYSA-N 0.000 description 2
XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 2
241000242759 Actiniaria Species 0.000 description 2
229950006790 Adenosine phosphate Drugs 0.000 description 2
229940064734 Aminobenzoate Drugs 0.000 description 2
241000269328 Amphibia Species 0.000 description 2
241000242757 Anthozoa Species 0.000 description 2
229940064005 Antibiotic throat preparations Drugs 0.000 description 2
229940083879 Antibiotics FOR TREATMENT OF HEMORRHOIDS AND ANAL FISSURES FOR TOPICAL USE Drugs 0.000 description 2
229940042052 Antibiotics for systemic use Drugs 0.000 description 2
229940042786 Antitubercular Antibiotics Drugs 0.000 description 2
241000971686 Argyropelecus Species 0.000 description 2
241000238421 Arthropoda Species 0.000 description 2
101710010421 BN863_22020 Proteins 0.000 description 2
101710010411 BN863_22030 Proteins 0.000 description 2
241000283690 Bos taurus Species 0.000 description 2
210000004556 Brain Anatomy 0.000 description 2
102000000584 Calmodulin Human genes 0.000 description 2
108010041952 Calmodulin Proteins 0.000 description 2
210000001736 Capillaries Anatomy 0.000 description 2
235000014653 Carica parviflora Nutrition 0.000 description 2
210000000170 Cell Membrane Anatomy 0.000 description 2
241000238366 Cephalopoda Species 0.000 description 2
241000881619 Chiroteuthis Species 0.000 description 2
239000004971 Cross linker Substances 0.000 description 2
WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
238000000018 DNA microarray Methods 0.000 description 2
241000199914 Dinophyceae Species 0.000 description 2
108010092799 EC 2.7.7.49 Proteins 0.000 description 2
108060000200 EC 4.6.1.1 Proteins 0.000 description 2
102000019460 EC 4.6.1.1 Human genes 0.000 description 2
241000701867 Enterobacteria phage T7 Species 0.000 description 2
241000701959 Escherichia virus Lambda Species 0.000 description 2
241000980703 Eucleoteuthis Species 0.000 description 2
208000009745 Eye Disease Diseases 0.000 description 2
241000661303 Florenciella Species 0.000 description 2
229940093922 Gynecological Antibiotics Drugs 0.000 description 2
108010054147 Hemoglobins Proteins 0.000 description 2
102000001554 Hemoglobins Human genes 0.000 description 2
241000271834 Howella Species 0.000 description 2
229960000310 ISOLEUCINE Drugs 0.000 description 2
102000018358 Immunoglobulins Human genes 0.000 description 2
108060003951 Immunoglobulins Proteins 0.000 description 2
241000500891 Insecta Species 0.000 description 2
150000008575 L-amino acids Chemical group 0.000 description 2
XUJNEKJLAYXESH-REOHCLBHSA-N L-cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 2
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
241001290159 Malacosteus Species 0.000 description 2
241000361919 Metaphire sieboldi Species 0.000 description 2
OSWPMRLSEDHDFF-UHFFFAOYSA-N Methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 description 2
LXCFILQKKLGQFO-UHFFFAOYSA-N Methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 2
BQJCRHHNABKAKU-KBQPJGBKSA-N Morphine Chemical compound O([C@H]1[C@H](C=C[C@H]23)O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4O BQJCRHHNABKAKU-KBQPJGBKSA-N 0.000 description 2
229940051866 Mouthwash Drugs 0.000 description 2
101700080605 NUC1 Proteins 0.000 description 2
229920000272 Oligonucleotide Polymers 0.000 description 2
241000882340 Onychoteuthis Species 0.000 description 2
241001443978 Oplophorus Species 0.000 description 2
210000003463 Organelles Anatomy 0.000 description 2
241000283898 Ovis Species 0.000 description 2
108090000854 Oxidoreductases Proteins 0.000 description 2
102000004316 Oxidoreductases Human genes 0.000 description 2
241000512292 Pholas Species 0.000 description 2
241000235648 Pichia Species 0.000 description 2
238000002123 RNA extraction Methods 0.000 description 2
102000007312 Recombinant Proteins Human genes 0.000 description 2
108010033725 Recombinant Proteins Proteins 0.000 description 2
241000235070 Saccharomyces Species 0.000 description 2
241001002152 Sepiolina Species 0.000 description 2
241000927894 Sergestes Species 0.000 description 2
210000002966 Serum Anatomy 0.000 description 2
108010071390 Serum Albumin Proteins 0.000 description 2
108020004682 Single-Stranded DNA Proteins 0.000 description 2
210000002784 Stomach Anatomy 0.000 description 2
229940024982 Topical Antifungal Antibiotics Drugs 0.000 description 2
241000700605 Viruses Species 0.000 description 2
241000238430 Watasenia Species 0.000 description 2
241000269368 Xenopus laevis Species 0.000 description 2
230000002378 acidificating Effects 0.000 description 2
230000004913 activation Effects 0.000 description 2
235000004279 alanine Nutrition 0.000 description 2
230000000735 allogeneic Effects 0.000 description 2
XXXHSQBVHSJQKS-UHFFFAOYSA-N amino benzoate Chemical compound NOC(=O)C1=CC=CC=C1 XXXHSQBVHSJQKS-UHFFFAOYSA-N 0.000 description 2
239000003242 anti bacterial agent Substances 0.000 description 2
125000003118 aryl group Chemical group 0.000 description 2
108010003152 bacteriophage T7 RNA polymerase Proteins 0.000 description 2
238000010256 biochemical assay Methods 0.000 description 2
230000003115 biocidal Effects 0.000 description 2
238000006065 biodegradation reaction Methods 0.000 description 2
230000036772 blood pressure Effects 0.000 description 2
238000004422 calculation algorithm Methods 0.000 description 2
201000011510 cancer Diseases 0.000 description 2
FUZZWVXGSFPDMH-UHFFFAOYSA-M caproate Chemical compound CCCCCC([O-])=O FUZZWVXGSFPDMH-UHFFFAOYSA-M 0.000 description 2
OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
125000004432 carbon atoms Chemical group C* 0.000 description 2
239000006229 carbon black Substances 0.000 description 2
CURLTUGMZLYLDI-UHFFFAOYSA-N carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
235000011089 carbon dioxide Nutrition 0.000 description 2
125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 2
239000003054 catalyst Substances 0.000 description 2
239000001913 cellulose Substances 0.000 description 2
235000010980 cellulose Nutrition 0.000 description 2
229920002678 cellulose Polymers 0.000 description 2
239000007806 chemical reaction intermediate Substances 0.000 description 2
230000001808 coupling Effects 0.000 description 2
238000010168 coupling process Methods 0.000 description 2
238000005859 coupling reaction Methods 0.000 description 2
239000003431 cross linking reagent Substances 0.000 description 2
238000005520 cutting process Methods 0.000 description 2
235000018417 cysteine Nutrition 0.000 description 2
125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 2
238000004090 dissolution Methods 0.000 description 2
239000000975 dye Substances 0.000 description 2
238000003379 elimination reaction Methods 0.000 description 2
230000002255 enzymatic Effects 0.000 description 2
150000002148 esters Chemical class 0.000 description 2
239000004744 fabric Substances 0.000 description 2
239000000796 flavoring agent Substances 0.000 description 2
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
235000003599 food sweetener Nutrition 0.000 description 2
230000002538 fungal Effects 0.000 description 2
238000001502 gel electrophoresis Methods 0.000 description 2
230000002068 genetic Effects 0.000 description 2
239000008103 glucose Substances 0.000 description 2
150000004676 glycans Polymers 0.000 description 2
125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 2
238000000227 grinding Methods 0.000 description 2
238000004128 high performance liquid chromatography Methods 0.000 description 2
239000000710 homodimer Substances 0.000 description 2
238000002657 hormone replacement therapy Methods 0.000 description 2
229910052739 hydrogen Inorganic materials 0.000 description 2
230000002163 immunogen Effects 0.000 description 2
239000007924 injection Substances 0.000 description 2
238000002347 injection Methods 0.000 description 2
229940079866 intestinal antibiotics Drugs 0.000 description 2
238000007918 intramuscular administration Methods 0.000 description 2
238000004255 ion exchange chromatography Methods 0.000 description 2
125000000012 isoleucine group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 description 2
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl β-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
231100000053 low toxicity Toxicity 0.000 description 2
239000000314 lubricant Substances 0.000 description 2
101700002549 luxA Proteins 0.000 description 2
101700041054 luxB Proteins 0.000 description 2
HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
210000004962 mammalian cells Anatomy 0.000 description 2
238000005259 measurement Methods 0.000 description 2
108020004084 membrane receptors Proteins 0.000 description 2
239000011325 microbead Substances 0.000 description 2
238000002156 mixing Methods 0.000 description 2
238000010369 molecular cloning Methods 0.000 description 2
230000004001 molecular interaction Effects 0.000 description 2
229960005181 morphine Drugs 0.000 description 2
229930014694 morphine Natural products 0.000 description 2
239000002324 mouth wash Substances 0.000 description 2
230000001537 neural Effects 0.000 description 2
230000001264 neutralization Effects 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
101700006494 nucA Proteins 0.000 description 2
239000002777 nucleoside Substances 0.000 description 2
210000000287 oocyte Anatomy 0.000 description 2
229940005935 ophthalmologic Antibiotics Drugs 0.000 description 2
238000005457 optimization Methods 0.000 description 2
239000002245 particle Substances 0.000 description 2
239000008188 pellet Substances 0.000 description 2
239000008194 pharmaceutical composition Substances 0.000 description 2
108060006184 phycobiliprotein family Proteins 0.000 description 2
108091008117 polyclonal antibodies Proteins 0.000 description 2
229920001282 polysaccharide Polymers 0.000 description 2
239000005017 polysaccharide Substances 0.000 description 2
150000004804 polysaccharides Polymers 0.000 description 2
235000020004 porter Nutrition 0.000 description 2
WCUXLLCKKVVCTQ-UHFFFAOYSA-M potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
DNIAPMSPPWPWGF-UHFFFAOYSA-N propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 2
230000002633 protecting Effects 0.000 description 2
238000010188 recombinant method Methods 0.000 description 2
230000008929 regeneration Effects 0.000 description 2
238000011069 regeneration method Methods 0.000 description 2
230000028327 secretion Effects 0.000 description 2
238000004062 sedimentation Methods 0.000 description 2
VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
XUIMIQQOPSSXEZ-UHFFFAOYSA-N silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
229910052710 silicon Inorganic materials 0.000 description 2
239000010703 silicon Substances 0.000 description 2
150000003384 small molecules Chemical class 0.000 description 2
238000005507 spraying Methods 0.000 description 2
238000003860 storage Methods 0.000 description 2
239000005720 sucrose Substances 0.000 description 2
150000008163 sugars Chemical class 0.000 description 2
230000004083 survival Effects 0.000 description 2
239000003765 sweetening agent Substances 0.000 description 2
239000006188 syrup Substances 0.000 description 2
238000002560 therapeutic procedure Methods 0.000 description 2
238000004809 thin layer chromatography Methods 0.000 description 2
RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 2
230000005026 transcription initiation Effects 0.000 description 2
230000002103 transcriptional Effects 0.000 description 2
238000001890 transfection Methods 0.000 description 2
230000032258 transport Effects 0.000 description 2
239000001226 triphosphate Substances 0.000 description 2
235000011178 triphosphate Nutrition 0.000 description 2
241000701447 unidentified baculovirus Species 0.000 description 2
229960005486 vaccines Drugs 0.000 description 2
239000004474 valine Substances 0.000 description 2
125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 2
VYEKJNGRMIAFMH-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-(1-pyridin-2-ylsulfanylethyl)benzoate Chemical compound C=1C=C(C(=O)ON2C(CCC2=O)=O)C=CC=1C(C)SC1=CC=CC=N1 VYEKJNGRMIAFMH-UHFFFAOYSA-N 0.000 description 1
BQWBEDSJTMWJAE-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-[(2-iodoacetyl)amino]benzoate Chemical compound C1=CC(NC(=O)CI)=CC=C1C(=O)ON1C(=O)CCC1=O BQWBEDSJTMWJAE-UHFFFAOYSA-N 0.000 description 1
GKSPIZSKQWTXQG-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-[1-(pyridin-2-yldisulfanyl)ethyl]benzoate Chemical compound C=1C=C(C(=O)ON2C(CCC2=O)=O)C=CC=1C(C)SSC1=CC=CC=N1 GKSPIZSKQWTXQG-UHFFFAOYSA-N 0.000 description 1
QYEAAMBIUQLHFQ-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 6-[3-(pyridin-2-yldisulfanyl)propanoylamino]hexanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCCCCNC(=O)CCSSC1=CC=CC=N1 QYEAAMBIUQLHFQ-UHFFFAOYSA-N 0.000 description 1
NWHMETIBESABNA-UHFFFAOYSA-N 1-[2-(7-azido-4-methyl-2-oxochromen-3-yl)acetyl]oxy-2,5-dioxopyrrolidine-3-sulfonic acid Chemical compound O=C1OC=2C=C(N=[N+]=[N-])C=CC=2C(C)=C1CC(=O)ON1C(=O)CC(S(O)(=O)=O)C1=O NWHMETIBESABNA-UHFFFAOYSA-N 0.000 description 1
NVKAWKQGWWIWPM-ABEVXSGRSA-N 17-β-hydroxy-5-α-Androstan-3-one Chemical compound C1C(=O)CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CC[C@H]21 NVKAWKQGWWIWPM-ABEVXSGRSA-N 0.000 description 1
ZKAMEFMDQNTDFK-UHFFFAOYSA-N 1H-imidazo[4,5-b]pyrazine Chemical compound C1=CN=C2NC=NC2=N1 ZKAMEFMDQNTDFK-UHFFFAOYSA-N 0.000 description 1
XXUNIGZDNWWYED-UHFFFAOYSA-N 2-methylbenzamide Chemical compound CC1=CC=CC=C1C(N)=O XXUNIGZDNWWYED-UHFFFAOYSA-N 0.000 description 1
JMUAKWNHKQBPGJ-UHFFFAOYSA-N 3-(pyridin-2-yldisulfanyl)-N-[4-[3-(pyridin-2-yldisulfanyl)propanoylamino]butyl]propanamide Chemical compound C=1C=CC=NC=1SSCCC(=O)NCCCCNC(=O)CCSSC1=CC=CC=N1 JMUAKWNHKQBPGJ-UHFFFAOYSA-N 0.000 description 1
101710027066 ALB Proteins 0.000 description 1
241000006840 Acanthoptilum Species 0.000 description 1
229960003190 Adenosine Monophosphate Drugs 0.000 description 1
229960001456 Adenosine Triphosphate Drugs 0.000 description 1
IBVAQQYNSHJXBV-UHFFFAOYSA-N Adipic acid dihydrazide Chemical compound NNC(=O)CCCCC(=O)NN IBVAQQYNSHJXBV-UHFFFAOYSA-N 0.000 description 1
241000426851 Aequorea aequorea Species 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
241000124001 Alcyonacea Species 0.000 description 1
XJKJWTWGDGIQRH-BFIDDRIFSA-N Alginic acid Chemical compound O1[C@@H](C(O)=O)[C@@H](OC)[C@H](O)[C@H](O)[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](C)[C@@H](O)[C@H]1O XJKJWTWGDGIQRH-BFIDDRIFSA-N 0.000 description 1
241000607620 Aliivibrio fischeri Species 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N Ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
241000017813 Amphora sulcata Species 0.000 description 1
241001124076 Aphididae Species 0.000 description 1
241001648766 Apogon <genus> Species 0.000 description 1
229940114079 Arachidonic Acid Drugs 0.000 description 1
YZXBAPSDXZZRGB-DOFZRALJSA-N Arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 1
241000239223 Arachnida Species 0.000 description 1
241001331189 Aristostomias scintillans Species 0.000 description 1
229960001230 Asparagine Drugs 0.000 description 1
229960005261 Aspartic Acid Drugs 0.000 description 1
241000416162 Astragalus gummifer Species 0.000 description 1
206010003816 Autoimmune disease Diseases 0.000 description 1
241000271566 Aves Species 0.000 description 1
IOJUPLGTWVMSFF-UHFFFAOYSA-N Benzothiazole Chemical compound C1=CC=C2SC=NC2=C1 IOJUPLGTWVMSFF-UHFFFAOYSA-N 0.000 description 1
241000237519 Bivalvia Species 0.000 description 1
210000000988 Bone and Bones Anatomy 0.000 description 1
108091003117 Bovine Serum Albumin Proteins 0.000 description 1
241000195940 Bryophyta Species 0.000 description 1
125000003860 C1-C20 alkoxy group Chemical group 0.000 description 1
101700045490 CALBP Proteins 0.000 description 1
101710006045 CREBBP Proteins 0.000 description 1
241000283707 Capra Species 0.000 description 1
208000005623 Carcinogenesis Diseases 0.000 description 1
229940106189 Ceramides Drugs 0.000 description 1
229940112822 Chewing Gum Drugs 0.000 description 1
229920002101 Chitin Polymers 0.000 description 1
DJHJJVWPFGHIPH-OODMECLYSA-N Chitin Chemical compound O[C@@H]1C(NC(=O)C)[C@H](O)OC(CO)[C@H]1COC[C@H]1C(NC(C)=O)[C@@H](O)[C@H](COC[C@H]2C([C@@H](O)[C@H](O)C(CO)O2)NC(C)=O)C(CO)O1 DJHJJVWPFGHIPH-OODMECLYSA-N 0.000 description 1
241000282552 Chlorocebus aethiops Species 0.000 description 1
241000006720 Clavularia sp. Species 0.000 description 1
210000000078 Claw Anatomy 0.000 description 1
JIAUJOOGSKJAFH-UHFFFAOYSA-N Coelenteramide Chemical compound C1=CC(O)=CC=C1CC(=O)NC(N=CC(N1)=C2C=CC(=O)C=C2)=C1CC1=CC=CC=C1 JIAUJOOGSKJAFH-UHFFFAOYSA-N 0.000 description 1
210000001520 Comb Anatomy 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
229920002261 Corn starch Polymers 0.000 description 1
241000699802 Cricetulus griseus Species 0.000 description 1
241000195493 Cryptophyta Species 0.000 description 1
241000219122 Cucurbita Species 0.000 description 1
241000432408 Curculio hilgendorfi Species 0.000 description 1
ZOOGRGPOEVQQDX-UUOKFMHZSA-N Cyclic guanosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-UUOKFMHZSA-N 0.000 description 1
NZNMSOFKMUBTKW-UHFFFAOYSA-N Cyclohexanecarboxylic acid Chemical compound OC(=O)C1CCCCC1 NZNMSOFKMUBTKW-UHFFFAOYSA-N 0.000 description 1
210000000805 Cytoplasm Anatomy 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N D-sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
238000007399 DNA isolation Methods 0.000 description 1
101700011961 DPOM Proteins 0.000 description 1
KSMVZQYAVGTKIV-UHFFFAOYSA-N Decanal Chemical compound CCCCCCCCCC=O KSMVZQYAVGTKIV-UHFFFAOYSA-N 0.000 description 1
210000003298 Dental Enamel Anatomy 0.000 description 1
229920002307 Dextran Polymers 0.000 description 1
241000006271 Discosoma sp. Species 0.000 description 1
241001512730 Discosoma striata Species 0.000 description 1
241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
229940022766 EGTA Drugs 0.000 description 1
101710003775 ERVK-10 Proteins 0.000 description 1
101710037030 ERVK-11 Proteins 0.000 description 1
101710009283 ERVK-18 Proteins 0.000 description 1
101710009286 ERVK-19 Proteins 0.000 description 1
101710035700 ERVK-25 Proteins 0.000 description 1
101710038044 ERVK-6 Proteins 0.000 description 1
101710014468 ERVK-7 Proteins 0.000 description 1
101710014482 ERVK-8 Proteins 0.000 description 1
241000258955 Echinodermata Species 0.000 description 1
241001427543 Elateridae Species 0.000 description 1
241000283073 Equus caballus Species 0.000 description 1
241000206602 Eukaryota Species 0.000 description 1
101710041084 FCABP Proteins 0.000 description 1
241000272184 Falconiformes Species 0.000 description 1
241000724791 Filamentous phage Species 0.000 description 1
210000004905 Finger nails Anatomy 0.000 description 1
235000019733 Fish meal Nutrition 0.000 description 1
101700040698 GFPL1 Proteins 0.000 description 1
101700026696 GFPL2 Proteins 0.000 description 1
101700014779 GLB1 Proteins 0.000 description 1
241000287828 Gallus gallus Species 0.000 description 1
241001343649 Gaussia princeps (T. Scott, 1894) Species 0.000 description 1
229960002989 Glutamic Acid Drugs 0.000 description 1
241000695276 Glyphus Species 0.000 description 1
101710043924 HERVK_113 Proteins 0.000 description 1
210000004408 Hybridomas Anatomy 0.000 description 1
108090000745 Immune Sera Proteins 0.000 description 1
102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
229960000367 Inositol Drugs 0.000 description 1
210000000936 Intestines Anatomy 0.000 description 1
102000004310 Ion Channels Human genes 0.000 description 1
108090000862 Ion Channels Proteins 0.000 description 1
241000764238 Isis Species 0.000 description 1
210000001503 Joints Anatomy 0.000 description 1
SBUJHOSQTJFQJX-NOAMYHISSA-N Kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
210000003734 Kidney Anatomy 0.000 description 1
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical group OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
125000000510 L-tryptophano group Chemical group [H]C1=C([H])C([H])=C2N([H])C([H])=C(C([H])([H])[C@@]([H])(C(O[H])=O)N([H])[*])C2=C1[H] 0.000 description 1
101700016645 LBP Proteins 0.000 description 1
GUBGYTABKSRVRQ-UUNJERMWSA-N Lactose Natural products O([C@@H]1[C@H](O)[C@H](O)[C@H](O)O[C@@H]1CO)[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1 GUBGYTABKSRVRQ-UUNJERMWSA-N 0.000 description 1
102000004856 Lectins Human genes 0.000 description 1
108090001090 Lectins Proteins 0.000 description 1
206010024324 Leukaemias Diseases 0.000 description 1
241000254056 Luciola Species 0.000 description 1
241001124207 Luciola mingrelica Species 0.000 description 1
241000013261 Lypoglossa lateralis Species 0.000 description 1
101710029649 MDV043 Proteins 0.000 description 1
241001290158 Malacosteus niger Species 0.000 description 1
240000006217 Mentha pulegium Species 0.000 description 1
235000016257 Mentha pulegium Nutrition 0.000 description 1
230000036650 Metabolic stability Effects 0.000 description 1
230000035633 Metabolized Effects 0.000 description 1
206010061289 Metastatic neoplasm Diseases 0.000 description 1
229920000168 Microcrystalline cellulose Polymers 0.000 description 1
210000004688 Microtubules Anatomy 0.000 description 1
102000028664 Microtubules Human genes 0.000 description 1
108091022031 Microtubules Proteins 0.000 description 1
210000004080 Milk Anatomy 0.000 description 1
210000004400 Mucous Membrane Anatomy 0.000 description 1
102000016943 Muramidase Human genes 0.000 description 1
108010014251 Muramidase Proteins 0.000 description 1
210000003205 Muscles Anatomy 0.000 description 1
235000013418 Myrtus communis Nutrition 0.000 description 1
240000005125 Myrtus communis Species 0.000 description 1
QVRVXSZKCXFBTE-UHFFFAOYSA-N N-[4-(6,7-dimethoxy-3,4-dihydro-1H-isoquinolin-2-yl)butyl]-2-(2-fluoroethoxy)-5-methylbenzamide Chemical compound C1C=2C=C(OC)C(OC)=CC=2CCN1CCCCNC(=O)C1=CC(C)=CC=C1OCCF QVRVXSZKCXFBTE-UHFFFAOYSA-N 0.000 description 1
SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 1
229940052665 NADH Drugs 0.000 description 1
241000018620 Neoscopelus Species 0.000 description 1
240000008962 Nicotiana tabacum Species 0.000 description 1
235000002637 Nicotiana tabacum Nutrition 0.000 description 1
241000200174 Noctiluca Species 0.000 description 1
239000004677 Nylon Substances 0.000 description 1
102000015636 Oligopeptides Human genes 0.000 description 1
108010038807 Oligopeptides Proteins 0.000 description 1
229940037201 Oris Drugs 0.000 description 1
241000283973 Oryctolagus cuniculus Species 0.000 description 1
241000238590 Ostracoda Species 0.000 description 1
210000001672 Ovary Anatomy 0.000 description 1
108020005203 Oxidases Proteins 0.000 description 1
101700061424 POLB Proteins 0.000 description 1
241000590428 Panacea Species 0.000 description 1
241000538436 Parapriacanthus Species 0.000 description 1
241000169268 Parazoanthus Species 0.000 description 1
108091005771 Peptidases Proteins 0.000 description 1
210000001322 Periplasm Anatomy 0.000 description 1
241000254058 Photinus Species 0.000 description 1
229920002732 Polyanhydride Polymers 0.000 description 1
229920000954 Polyglycolide Polymers 0.000 description 1
229920001710 Polyorthoester Polymers 0.000 description 1
239000004743 Polypropylene Substances 0.000 description 1
229920001451 Polypropylene glycol Polymers 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
241000191235 Porichthys Species 0.000 description 1
239000004365 Protease Substances 0.000 description 1
102000002067 Protein Subunits Human genes 0.000 description 1
108010001267 Protein Subunits Proteins 0.000 description 1
101700054624 RF1 Proteins 0.000 description 1
101710006375 RNASEH1 Proteins 0.000 description 1
101700078434 RT67 Proteins 0.000 description 1
108010052090 Renilla Luciferases Proteins 0.000 description 1
102000006382 Ribonucleases Human genes 0.000 description 1
108010083644 Ribonucleases Proteins 0.000 description 1
229920001914 Ribonucleotide Polymers 0.000 description 1
241000282849 Ruminantia Species 0.000 description 1
CSZNUDNYNSELMP-UHFFFAOYSA-M S(=S)(=O)(OC(C1=CC=CC=C1)(C)C(=O)ON1C(CCC1=O)=O)[O-] Chemical compound S(=S)(=O)(OC(C1=CC=CC=C1)(C)C(=O)ON1C(CCC1=O)=O)[O-] CSZNUDNYNSELMP-UHFFFAOYSA-M 0.000 description 1
101710018438 SDF4 Proteins 0.000 description 1
102100004824 SDF4 Human genes 0.000 description 1
CVHZOJJKTDOEJC-UHFFFAOYSA-N Saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
229940081974 Saccharin Drugs 0.000 description 1
230000037165 Serum Concentration Effects 0.000 description 1
241000863431 Shewanella hanedai Species 0.000 description 1
208000006641 Skin Disease Diseases 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
241001603947 Stomias Species 0.000 description 1
208000010513 Stupor Diseases 0.000 description 1
241000006838 Stylatula Species 0.000 description 1
CZMRCDWAGMRECN-GDQSFJPYSA-N Sucrose Natural products O([C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1)[C@@]1(CO)[C@H](O)[C@@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-GDQSFJPYSA-N 0.000 description 1
241000282887 Suidae Species 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical class OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
241000282898 Sus scrofa Species 0.000 description 1
241000143014 T7virus Species 0.000 description 1
229920001615 Tragacanth Polymers 0.000 description 1
102000002142 Trans-Activators Human genes 0.000 description 1
108010040939 Trans-Activators Proteins 0.000 description 1
239000007997 Tricine buffer Substances 0.000 description 1
241000556194 Vampyroteuthis infernalis Species 0.000 description 1
241000238583 Vargula hilgendorfii Species 0.000 description 1
241000251539 Vertebrata <Metazoa> Species 0.000 description 1
241001406754 Vinciguerria Species 0.000 description 1
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Vitamin C Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
108091005955 Wild-type GFP Proteins 0.000 description 1
241001512733 Zoanthus sp. Species 0.000 description 1
INULNSAIIZKOQE-YOSAUDMPSA-N [(3R,4aR,10aR)-6-methoxy-1-methyl-3,4,4a,5,10,10a-hexahydro-2H-benzo[g]quinolin-3-yl]-[4-(4-nitrophenyl)piperazin-1-yl]methanone Chemical compound O=C([C@@H]1C[C@H]2[C@H](N(C1)C)CC=1C=CC=C(C=1C2)OC)N(CC1)CCN1C1=CC=C([N+]([O-])=O)C=C1 INULNSAIIZKOQE-YOSAUDMPSA-N 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K [O-]P([O-])([O-])=O Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
ZKJOXOJMGXFSPF-QYZPTAICSA-N [[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2R,3S,4R,5R)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate;hydrate Chemical compound O.NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 ZKJOXOJMGXFSPF-QYZPTAICSA-N 0.000 description 1
BTXLWRWDYZKCPQ-UHFFFAOYSA-N [nitro(phenyl)methyl] carbonochloridate Chemical compound ClC(=O)OC([N+](=O)[O-])C1=CC=CC=C1 BTXLWRWDYZKCPQ-UHFFFAOYSA-N 0.000 description 1
150000001242 acetic acid derivatives Chemical class 0.000 description 1
239000002671 adjuvant Substances 0.000 description 1
230000000240 adjuvant Effects 0.000 description 1
238000001042 affinity chromatography Methods 0.000 description 1
238000003450 affinity purification method Methods 0.000 description 1
125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 1
229940050528 albumin Drugs 0.000 description 1
239000000783 alginic acid Substances 0.000 description 1
235000010443 alginic acid Nutrition 0.000 description 1
229920000615 alginic acid Polymers 0.000 description 1
229960001126 alginic acid Drugs 0.000 description 1
125000003342 alkenyl group Chemical group 0.000 description 1
230000002009 allergen Effects 0.000 description 1
230000004075 alteration Effects 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
230000003321 amplification Effects 0.000 description 1
239000003708 ampul Substances 0.000 description 1
230000000111 anti-oxidant Effects 0.000 description 1
239000004599 antimicrobial Substances 0.000 description 1
239000003963 antioxidant agent Substances 0.000 description 1
235000006708 antioxidants Nutrition 0.000 description 1
235000021342 arachidonic acid Nutrition 0.000 description 1
235000010323 ascorbic acid Nutrition 0.000 description 1
229960005070 ascorbic acid Drugs 0.000 description 1
239000011668 ascorbic acid Substances 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
239000012131 assay buffer Substances 0.000 description 1
238000003556 assay method Methods 0.000 description 1
235000019445 benzyl alcohol Nutrition 0.000 description 1
108020004256 beta-Lactamases Proteins 0.000 description 1
102000006635 beta-Lactamases Human genes 0.000 description 1
102000024070 binding proteins Human genes 0.000 description 1
108091007650 binding proteins Proteins 0.000 description 1
229920000249 biocompatible polymer Polymers 0.000 description 1
230000033228 biological regulation Effects 0.000 description 1
230000005540 biological transmission Effects 0.000 description 1
239000012503 blood component Substances 0.000 description 1
238000007664 blowing Methods 0.000 description 1
230000037396 body weight Effects 0.000 description 1
IJDNQMDRQITEOD-UHFFFAOYSA-N butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 1
239000001273 butane Substances 0.000 description 1
159000000007 calcium salts Chemical group 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
230000036952 cancer formation Effects 0.000 description 1
150000001735 carboxylic acids Chemical class 0.000 description 1
231100000504 carcinogenesis Toxicity 0.000 description 1
238000006555 catalytic reaction Methods 0.000 description 1
229920002092 cellular RNA Polymers 0.000 description 1
150000001783 ceramides Chemical class 0.000 description 1
238000001311 chemical methods and process Methods 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
238000003392 chemiluminescence resonance energy transfer Methods 0.000 description 1
235000015218 chewing gum Nutrition 0.000 description 1
235000019804 chlorophyll Nutrition 0.000 description 1
ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
229930002875 chlorophylls Natural products 0.000 description 1
229960004316 cisplatin Drugs 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
239000008199 coating composition Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
239000000084 colloidal system Substances 0.000 description 1
238000003271 compound fluorescence assay Methods 0.000 description 1
238000004590 computer program Methods 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
235000008504 concentrate Nutrition 0.000 description 1
230000021615 conjugation Effects 0.000 description 1
210000000555 contractile cell Anatomy 0.000 description 1
239000008120 corn starch Substances 0.000 description 1
210000004748 cultured cells Anatomy 0.000 description 1
125000004122 cyclic group Chemical group 0.000 description 1
VOLSCWDWGMWXGO-UHFFFAOYSA-N cyclobuten-1-yl acetate Chemical compound CC(=O)OC1=CCC1 VOLSCWDWGMWXGO-UHFFFAOYSA-N 0.000 description 1
230000009089 cytolysis Effects 0.000 description 1
229920002093 cytoplasmic RNA Polymers 0.000 description 1
230000001086 cytosolic Effects 0.000 description 1
230000003013 cytotoxicity Effects 0.000 description 1
231100000135 cytotoxicity Toxicity 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
239000000551 dentifrice Substances 0.000 description 1
239000005547 deoxyribonucleotide Substances 0.000 description 1
125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
230000036576 dermal application Effects 0.000 description 1
235000014113 dietary fatty acids Nutrition 0.000 description 1
230000029087 digestion Effects 0.000 description 1
239000003085 diluting agent Substances 0.000 description 1
238000010790 dilution Methods 0.000 description 1
238000006471 dimerization reaction Methods 0.000 description 1
BVTJGGGYKAMDBN-UHFFFAOYSA-N dioxetane Chemical class C1COO1 BVTJGGGYKAMDBN-UHFFFAOYSA-N 0.000 description 1
150000002016 disaccharides Chemical class 0.000 description 1
239000002270 dispersing agent Substances 0.000 description 1
238000010494 dissociation reaction Methods 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
150000002019 disulfides Chemical class 0.000 description 1
125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
230000036267 drug metabolism Effects 0.000 description 1
238000007877 drug screening Methods 0.000 description 1
241001233061 earthworms Species 0.000 description 1
201000003511 ectopic pregnancy Diseases 0.000 description 1
230000002500 effect on skin Effects 0.000 description 1
239000000839 emulsion Substances 0.000 description 1
230000002357 endometrial Effects 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
239000003623 enhancer Substances 0.000 description 1
239000002702 enteric coating Substances 0.000 description 1
238000009505 enteric coating Methods 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
239000005038 ethylene vinyl acetate Substances 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
239000000194 fatty acid Substances 0.000 description 1
150000004665 fatty acids Chemical group 0.000 description 1
239000010685 fatty oil Substances 0.000 description 1
230000001605 fetal Effects 0.000 description 1
239000004467 fishmeal Substances 0.000 description 1
235000019634 flavors Nutrition 0.000 description 1
235000013355 food flavoring agent Nutrition 0.000 description 1
239000008369 fruit flavor Substances 0.000 description 1
239000007903 gelatin capsule Substances 0.000 description 1
238000001415 gene therapy Methods 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
239000003365 glass fiber Substances 0.000 description 1
235000013922 glutamic acid Nutrition 0.000 description 1
239000004220 glutamic acid Chemical group 0.000 description 1
PEDCQBHIVMGVHV-UHFFFAOYSA-N glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
235000011187 glycerol Nutrition 0.000 description 1
230000005283 ground state Effects 0.000 description 1
200000000028 growth disorder Diseases 0.000 description 1
239000003102 growth factor Substances 0.000 description 1
239000000118 hair dye Substances 0.000 description 1
230000003760 hair shine Effects 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
150000002391 heterocyclic compounds Chemical class 0.000 description 1
235000001050 hortel pimenta Nutrition 0.000 description 1
229920002674 hyaluronan Polymers 0.000 description 1
229960003160 hyaluronic acid Drugs 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
125000004435 hydrogen atoms Chemical class [H]* 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
230000005660 hydrophilic surface Effects 0.000 description 1
230000002209 hydrophobic Effects 0.000 description 1
230000005661 hydrophobic surface Effects 0.000 description 1
238000005805 hydroxylation reaction Methods 0.000 description 1
150000005235 imidazopyrazines Chemical class 0.000 description 1
230000028993 immune response Effects 0.000 description 1
238000001114 immunoprecipitation Methods 0.000 description 1
239000007943 implant Substances 0.000 description 1
239000012535 impurity Substances 0.000 description 1
230000002779 inactivation Effects 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
239000003701 inert diluent Substances 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
230000000977 initiatory Effects 0.000 description 1
230000000968 intestinal Effects 0.000 description 1
238000003402 intramolecular cyclocondensation reaction Methods 0.000 description 1
238000007913 intrathecal administration Methods 0.000 description 1
239000000644 isotonic solution Substances 0.000 description 1
238000005304 joining Methods 0.000 description 1
229960000318 kanamycin Drugs 0.000 description 1
238000002372 labelling Methods 0.000 description 1
239000008101 lactose Substances 0.000 description 1
GUBGYTABKSRVRQ-XLOQQCSPSA-N lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
238000002350 laparotomy Methods 0.000 description 1
230000002045 lasting Effects 0.000 description 1
239000002523 lectin Substances 0.000 description 1
238000011068 load Methods 0.000 description 1
230000007762 localization of cell Effects 0.000 description 1
108060004501 luxC Proteins 0.000 description 1
108060004502 luxD Proteins 0.000 description 1
108060004503 luxE Proteins 0.000 description 1
101700035425 luxI Proteins 0.000 description 1
239000008176 lyophilized powder Substances 0.000 description 1
230000002934 lysing Effects 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
235000019359 magnesium stearate Nutrition 0.000 description 1
125000005439 maleimidyl group Chemical group C1(C=CC(N1*)=O)=O 0.000 description 1
102000006240 membrane receptors Human genes 0.000 description 1
230000004060 metabolic process Effects 0.000 description 1
239000002207 metabolite Substances 0.000 description 1
230000001394 metastastic Effects 0.000 description 1
229960000485 methotrexate Drugs 0.000 description 1
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
229960001047 methyl salicylate Drugs 0.000 description 1
229960002216 methylparaben Drugs 0.000 description 1
239000003094 microcapsule Substances 0.000 description 1
235000019813 microcrystalline cellulose Nutrition 0.000 description 1
239000008108 microcrystalline cellulose Substances 0.000 description 1
229940016286 microcrystalline cellulose Drugs 0.000 description 1
235000013336 milk Nutrition 0.000 description 1
239000008267 milk Substances 0.000 description 1
230000003278 mimic Effects 0.000 description 1
239000003607 modifier Substances 0.000 description 1
239000003068 molecular probe Substances 0.000 description 1
239000000178 monomer Substances 0.000 description 1
150000002772 monosaccharides Chemical class 0.000 description 1
235000007708 morin Nutrition 0.000 description 1
238000010172 mouse model Methods 0.000 description 1
235000011929 mousse Nutrition 0.000 description 1
239000006225 natural substrate Substances 0.000 description 1
229960004927 neomycin Drugs 0.000 description 1
125000006502 nitrobenzyl group Chemical group 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
239000000346 nonvolatile oil Substances 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
150000003833 nucleoside derivatives Chemical class 0.000 description 1
229920001778 nylon Polymers 0.000 description 1
108010089433 obelin Proteins 0.000 description 1
239000002357 osmotic agent Substances 0.000 description 1
230000037361 pathway Effects 0.000 description 1
235000006678 peppermint Nutrition 0.000 description 1
235000015132 peppermint Nutrition 0.000 description 1
235000007735 peppermint Nutrition 0.000 description 1
229940021222 peritoneal dialysis Isotonic solutions Drugs 0.000 description 1
150000002978 peroxides Chemical class 0.000 description 1
125000000864 peroxy group Chemical group O(O*)* 0.000 description 1
230000002085 persistent Effects 0.000 description 1
239000007793 ph indicator Substances 0.000 description 1
230000000275 pharmacokinetic Effects 0.000 description 1
239000012071 phase Substances 0.000 description 1
125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
238000006366 phosphorylation reaction Methods 0.000 description 1
230000000865 phosphorylative Effects 0.000 description 1
230000000886 photobiology Effects 0.000 description 1
230000000704 physical effect Effects 0.000 description 1
230000001766 physiological effect Effects 0.000 description 1
239000000049 pigment Substances 0.000 description 1
239000006187 pill Substances 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920001200 poly(ethylene-vinyl acetate) Polymers 0.000 description 1
230000001402 polyadenylating Effects 0.000 description 1
229920000515 polycarbonate Polymers 0.000 description 1
239000004417 polycarbonate Substances 0.000 description 1
239000004633 polyglycolic acid Substances 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
229920001155 polypropylene Polymers 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
229920001343 polytetrafluoroethylene Polymers 0.000 description 1
239000004810 polytetrafluoroethylene Substances 0.000 description 1
229910052700 potassium Inorganic materials 0.000 description 1
239000001103 potassium chloride Substances 0.000 description 1
235000011164 potassium chloride Nutrition 0.000 description 1
239000003755 preservative agent Substances 0.000 description 1
239000000651 prodrug Substances 0.000 description 1
229940002612 prodrugs Drugs 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
230000001737 promoting Effects 0.000 description 1
239000003380 propellant Substances 0.000 description 1
125000006239 protecting group Chemical group 0.000 description 1
230000036678 protein binding Effects 0.000 description 1
230000018883 protein targeting Effects 0.000 description 1
230000002797 proteolythic Effects 0.000 description 1
239000008262 pumice Substances 0.000 description 1
150000003212 purines Chemical class 0.000 description 1
238000003908 quality control method Methods 0.000 description 1
238000011002 quantification Methods 0.000 description 1
238000002708 random mutagenesis Methods 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
108091007521 restriction endonucleases Proteins 0.000 description 1
230000001177 retroviral Effects 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
101700086982 rnh Proteins 0.000 description 1
235000019204 saccharin Nutrition 0.000 description 1
239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 1
239000004576 sand Substances 0.000 description 1
238000009738 saturating Methods 0.000 description 1
239000004065 semiconductor Substances 0.000 description 1
230000019491 signal transduction Effects 0.000 description 1
239000000377 silicon dioxide Substances 0.000 description 1
235000012239 silicon dioxide Nutrition 0.000 description 1
239000002884 skin cream Substances 0.000 description 1
DWAQJAXMDSEUJJ-UHFFFAOYSA-M sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 1
229940001607 sodium bisulfite Drugs 0.000 description 1
235000010267 sodium hydrogen sulphite Nutrition 0.000 description 1
MAKUBRYLFHZREJ-JWBQXVCJSA-M sodium;(2S,3S,4R,5R,6R)-3-[(2S,3R,5S,6R)-3-acetamido-5-hydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4,5,6-trihydroxyoxane-2-carboxylate Chemical compound [Na+].CC(=O)N[C@@H]1C[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](C([O-])=O)O[C@@H](O)[C@H](O)[C@H]1O MAKUBRYLFHZREJ-JWBQXVCJSA-M 0.000 description 1
HHSGWIABCIVPJT-UHFFFAOYSA-M sodium;1-[4-[(2-iodoacetyl)amino]benzoyl]oxy-2,5-dioxopyrrolidine-3-sulfonate Chemical compound [Na+].O=C1C(S(=O)(=O)[O-])CC(=O)N1OC(=O)C1=CC=C(NC(=O)CI)C=C1 HHSGWIABCIVPJT-UHFFFAOYSA-M 0.000 description 1
ZAPNXDUFCQIHFS-UHFFFAOYSA-M sodium;2,5-dioxo-1-[6-[3-(pyridin-2-yldisulfanyl)propanoylamino]hexanoyloxy]pyrrolidine-3-sulfonate Chemical compound [Na+].O=C1C(S(=O)(=O)[O-])CC(=O)N1OC(=O)CCCCCNC(=O)CCSSC1=CC=CC=N1 ZAPNXDUFCQIHFS-UHFFFAOYSA-M 0.000 description 1
210000004872 soft tissue Anatomy 0.000 description 1
238000010532 solid phase synthesis reaction Methods 0.000 description 1
238000005063 solubilization Methods 0.000 description 1
239000012453 solvate Substances 0.000 description 1
210000004215 spores Anatomy 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
239000008174 sterile solution Substances 0.000 description 1
CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
229910052712 strontium Inorganic materials 0.000 description 1
230000004960 subcellular localization Effects 0.000 description 1
150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
229920002994 synthetic fiber Polymers 0.000 description 1
235000020357 syrup Nutrition 0.000 description 1
238000007910 systemic administration Methods 0.000 description 1
230000002123 temporal effect Effects 0.000 description 1
230000001225 therapeutic Effects 0.000 description 1
239000002562 thickening agent Substances 0.000 description 1
150000003573 thiols Chemical class 0.000 description 1
230000001988 toxicity Effects 0.000 description 1
231100000419 toxicity Toxicity 0.000 description 1
230000002110 toxicologic Effects 0.000 description 1
231100000759 toxicological effect Toxicity 0.000 description 1
239000000700 tracer Substances 0.000 description 1
230000001131 transforming Effects 0.000 description 1
230000001052 transient Effects 0.000 description 1
230000014621 translational initiation Effects 0.000 description 1
LXZZYRPGZAFOLE-UHFFFAOYSA-L transplatin Chemical compound [H][N]([H])([H])[Pt](Cl)(Cl)[N]([H])([H])[H] LXZZYRPGZAFOLE-UHFFFAOYSA-L 0.000 description 1
125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
239000010981 turquoise Substances 0.000 description 1
238000004450 types of analysis Methods 0.000 description 1
230000002792 vascular Effects 0.000 description 1
235000012431 wafers Nutrition 0.000 description 1
239000008215 water for injection Substances 0.000 description 1
101710010882 ypwA Proteins 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N β-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1

JP2001567308A 2000-03-15 2001-03-15 Ｒｅｎｉｌｌａｒｅｎｉｆｏｒｍｉｓ蛍光タンパク質、その蛍光タンパク質をコードする核酸、および診断、ハイスループットスクリーニングおよび新規アイテムにおけるその使用 Expired - Fee Related JP4830063B2 (ja)