JP2003510054A5 - - Google Patents
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- JP2003510054A5 JP2003510054A5 JP2001525396A JP2001525396A JP2003510054A5 JP 2003510054 A5 JP2003510054 A5 JP 2003510054A5 JP 2001525396 A JP2001525396 A JP 2001525396A JP 2001525396 A JP2001525396 A JP 2001525396A JP 2003510054 A5 JP2003510054 A5 JP 2003510054A5
- Authority
- JP
- Japan
- Prior art keywords
- oligonucleotide
- nucleic acid
- nucleotide
- probe
- hybridization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004707 nucleic acids Proteins 0.000 description 73
- 150000007523 nucleic acids Chemical class 0.000 description 73
- 239000002773 nucleotide Substances 0.000 description 60
- 125000003729 nucleotide group Chemical group 0.000 description 59
- 229920000272 Oligonucleotide Polymers 0.000 description 55
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 40
- 239000002751 oligonucleotide probe Substances 0.000 description 40
- 238000009396 hybridization Methods 0.000 description 35
- 239000000523 sample Substances 0.000 description 29
- 238000002493 microarray Methods 0.000 description 26
- 229920000642 polymer Polymers 0.000 description 26
- 241000894007 species Species 0.000 description 24
- 239000012472 biological sample Substances 0.000 description 22
- 125000005647 linker group Chemical group 0.000 description 21
- 239000000758 substrate Substances 0.000 description 18
- 238000006243 chemical reaction Methods 0.000 description 15
- 230000000295 complement Effects 0.000 description 15
- 229920001850 Nucleic acid sequence Polymers 0.000 description 12
- 230000035772 mutation Effects 0.000 description 12
- 239000007850 fluorescent dye Substances 0.000 description 9
- 239000000017 hydrogel Substances 0.000 description 9
- UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 230000001809 detectable Effects 0.000 description 7
- 206010071602 Genetic polymorphism Diseases 0.000 description 6
- 238000004166 bioassay Methods 0.000 description 5
- 238000010348 incorporation Methods 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 5
- -1 nucleotide triphosphates Chemical class 0.000 description 5
- 238000002966 oligonucleotide array Methods 0.000 description 5
- 230000002285 radioactive Effects 0.000 description 5
- 239000001226 triphosphate Substances 0.000 description 5
- 235000011178 triphosphate Nutrition 0.000 description 5
- 238000000018 DNA microarray Methods 0.000 description 4
- HDRRAMINWIWTNU-NTSWFWBYSA-N [[(2S,5R)-5-(2-amino-6-oxo-3H-purin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@H]1CC[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HDRRAMINWIWTNU-NTSWFWBYSA-N 0.000 description 4
- ARLKCWCREKRROD-POYBYMJQSA-N [[(2S,5R)-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)CC1 ARLKCWCREKRROD-POYBYMJQSA-N 0.000 description 4
- URGJWIFLBWJRMF-JGVFFNPUSA-N ddTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)CC1 URGJWIFLBWJRMF-JGVFFNPUSA-N 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 101700008821 EXO Proteins 0.000 description 3
- 101700083023 EXRN Proteins 0.000 description 3
- 229920001577 copolymer Polymers 0.000 description 3
- 229920003013 deoxyribonucleic acid Polymers 0.000 description 3
- 239000002831 pharmacologic agent Substances 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- OAKPWEUQDVLTCN-NKWVEPMBSA-N 2',3'-Dideoxyadenosine-5'-Triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1CC[C@@H](CO[P@@](O)(=O)O[P@](O)(=O)OP(O)(O)=O)O1 OAKPWEUQDVLTCN-NKWVEPMBSA-N 0.000 description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 229920000547 conjugated polymer Polymers 0.000 description 2
- 230000000875 corresponding Effects 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001215 fluorescent labelling Methods 0.000 description 2
- 201000002406 genetic disease Diseases 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000002458 infectious Effects 0.000 description 2
- 230000001404 mediated Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 230000003287 optical Effects 0.000 description 2
- 229920000128 polypyrrole Polymers 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- UNXRWKVEANCORM-UHFFFAOYSA-I triphosphate(5-) Chemical compound [O-]P([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O UNXRWKVEANCORM-UHFFFAOYSA-I 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- PTHCMJGKKRQCBF-UHFFFAOYSA-N Cellulose, microcrystalline Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC)C(CO)O1 PTHCMJGKKRQCBF-UHFFFAOYSA-N 0.000 description 1
- 101700011961 DPOM Proteins 0.000 description 1
- 229910001218 Gallium arsenide Inorganic materials 0.000 description 1
- 101710029649 MDV043 Proteins 0.000 description 1
- 235000000434 Melocanna baccifera Nutrition 0.000 description 1
- 241001497770 Melocanna baccifera Species 0.000 description 1
- 229910002656 O–Si–O Inorganic materials 0.000 description 1
- 101700061424 POLB Proteins 0.000 description 1
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 229920000265 Polyparaphenylene Polymers 0.000 description 1
- 101700054624 RF1 Proteins 0.000 description 1
- 229910052581 Si3N4 Inorganic materials 0.000 description 1
- HQVNEWCFYHHQES-UHFFFAOYSA-N Silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K Trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 125000003917 carbamoyl group Chemical class [H]N([H])C(*)=O 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000009910 diseases by infectious agent Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 150000002430 hydrocarbons Chemical group 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000009114 investigational therapy Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006011 modification reaction Methods 0.000 description 1
- TWXTWZIUMCFMSG-UHFFFAOYSA-N nitride(3-) Chemical compound [N-3] TWXTWZIUMCFMSG-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000553 poly(phenylenevinylene) Polymers 0.000 description 1
- 229920000767 polyaniline Polymers 0.000 description 1
- 229920001088 polycarbazole Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920002098 polyfluorene Polymers 0.000 description 1
- 229920000414 polyfuran Polymers 0.000 description 1
- 229920000123 polythiophene Polymers 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- KAESVJOAVNADME-UHFFFAOYSA-N pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 1
- 239000005060 rubber Substances 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 125000005373 siloxane group Chemical group [SiH2](O*)* 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing Effects 0.000 description 1
- 150000003568 thioethers Chemical group 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 239000011778 trisodium citrate Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15543199P | 1999-09-22 | 1999-09-22 | |
| US60/155,431 | 1999-09-22 | ||
| PCT/US2000/025988 WO2001021838A2 (en) | 1999-09-22 | 2000-09-22 | Three-dimensional microarray system for parallel genotyping of single nucleotide polymorphisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2003510054A JP2003510054A (ja) | 2003-03-18 |
| JP2003510054A5 true JP2003510054A5 (de) | 2007-11-08 |
Family
ID=22555401
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001525396A Pending JP2003510054A (ja) | 1999-09-22 | 2000-09-22 | 単一ヌクレオチド多形性の平行遺伝子型決定のための三次元ミクロアレイシステム |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1222310A2 (de) |
| JP (1) | JP2003510054A (de) |
| AU (1) | AU7708500A (de) |
| CA (1) | CA2385851A1 (de) |
| WO (1) | WO2001021838A2 (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1451357A4 (de) | 2001-11-01 | 2005-10-26 | Univ California | Biokatalytische sol-gel-mikroarrays |
| US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
| WO2008014485A2 (en) | 2006-07-28 | 2008-01-31 | California Institute Of Technology | Multiplex q-pcr arrays |
| US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US20120141986A1 (en) * | 2007-03-27 | 2012-06-07 | Kenneth Kuhn | Multivalent substrate elements for detection of nucleic acid sequences |
| EP2558859B1 (de) * | 2010-04-15 | 2017-09-27 | Digital Sensing Limited | Drei-dimensional mikroarrays |
| US9708647B2 (en) | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
| US9499861B1 (en) | 2015-09-10 | 2016-11-22 | Insilixa, Inc. | Methods and systems for multiplex quantitative nucleic acid amplification |
| WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
| EP3577233B1 (de) | 2017-03-31 | 2021-07-14 | Marcella Chiari | Genotypisierung von mutationen durch kombination von in-tube-hybridisierung und universellem tag-mikroarray |
| EP3937780A4 (de) | 2019-03-14 | 2022-12-07 | InSilixa, Inc. | Verfahren und systeme zur zeitgesteuerten fluoreszenzbasierten detektion |
| CN113943324B (zh) * | 2021-10-25 | 2023-07-28 | 中元汇吉生物技术股份有限公司 | 一种修饰的核苷酸，组合物及试剂 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3021036B2 (ja) * | 1989-02-13 | 2000-03-15 | ジェネコ・ピーティーワイ・リミテッド | 核酸配列又はその中の変化の検出 |
| RU1794088C (ru) * | 1991-03-18 | 1993-02-07 | Институт Молекулярной Биологии Ан@ Ссср | Способ определени нуклеотидной последовательности ДНК и устройство дл его осуществлени |
| WO1993025563A1 (en) * | 1992-06-17 | 1993-12-23 | City Of Hope | A method of detecting and discriminating between nucleic acid sequences |
| RU2041261C1 (ru) * | 1993-08-11 | 1995-08-09 | Институт молекулярной биологии им.В.А.Энгельгардта РАН | Способ изготовления матрицы для детектирования мисматчей |
| AUPO427996A0 (en) * | 1996-12-20 | 1997-01-23 | Co-Operative Research Centre For Diagnostic Technologies | Method for detecting a nucleotide at a specific location within a polynucleotide sequence and apparatus therefor |
| EP1498494A3 (de) * | 1997-04-01 | 2007-06-20 | Solexa Ltd. | Methode zur Sequenzierung von Nukleinsäuren |
| US6048692A (en) * | 1997-10-07 | 2000-04-11 | Motorola, Inc. | Sensors for electrically sensing binding events for supported molecular receptors |
| US6391937B1 (en) * | 1998-11-25 | 2002-05-21 | Motorola, Inc. | Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers |
-
2000
- 2000-09-22 EP EP00966797A patent/EP1222310A2/de not_active Withdrawn
- 2000-09-22 JP JP2001525396A patent/JP2003510054A/ja active Pending
- 2000-09-22 CA CA002385851A patent/CA2385851A1/en not_active Abandoned
- 2000-09-22 AU AU77085/00A patent/AU7708500A/en not_active Abandoned
- 2000-09-22 WO PCT/US2000/025988 patent/WO2001021838A2/en active Application Filing
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