JP2003505060A - リグニンデポリメラーゼの単離および活性 - Google Patents
リグニンデポリメラーゼの単離および活性Info
- Publication number
- JP2003505060A JP2003505060A JP2001512315A JP2001512315A JP2003505060A JP 2003505060 A JP2003505060 A JP 2003505060A JP 2001512315 A JP2001512315 A JP 2001512315A JP 2001512315 A JP2001512315 A JP 2001512315A JP 2003505060 A JP2003505060 A JP 2003505060A
- Authority
- JP
- Japan
- Prior art keywords
- lignin
- depolymerase
- polypeptide
- polymerized
- lignin depolymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 229920005610 lignin Polymers 0.000 title claims abstract description 107
- 238000002955 isolation Methods 0.000 title description 2
- 229920005611 kraft lignin Polymers 0.000 claims abstract description 21
- 239000002655 kraft paper Substances 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims description 25
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 25
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 25
- 229920001184 polypeptide Polymers 0.000 claims description 24
- 241000233866 Fungi Species 0.000 claims description 12
- 239000011122 softwood Substances 0.000 claims description 10
- 230000003698 anagen phase Effects 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 6
- 238000000338 in vitro Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- 241000688200 Cingulata Species 0.000 claims 1
- 239000000243 solution Substances 0.000 description 21
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 14
- 239000012528 membrane Substances 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 238000000108 ultra-filtration Methods 0.000 description 8
- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 description 6
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 description 6
- 239000011714 flavin adenine dinucleotide Substances 0.000 description 6
- 229940093632 flavin-adenine dinucleotide Drugs 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 241001230654 Trametes cingulata Species 0.000 description 5
- 238000010828 elution Methods 0.000 description 5
- 239000000706 filtrate Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 229920001131 Pulp (paper) Polymers 0.000 description 4
- 108020004511 Recombinant DNA Proteins 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000001155 isoelectric focusing Methods 0.000 description 4
- 108010091212 pepstatin Proteins 0.000 description 4
- FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 3
- 229920005654 Sephadex Polymers 0.000 description 3
- 239000012507 Sephadex™ Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 3
- 239000000123 paper Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 235000008565 Pinus banksiana Nutrition 0.000 description 2
- 241000218680 Pinus banksiana Species 0.000 description 2
- 239000008351 acetate buffer Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000004061 bleaching Methods 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000011491 glass wool Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 108091033319 polynucleotide Proteins 0.000 description 2
- 102000040430 polynucleotide Human genes 0.000 description 2
- 239000002157 polynucleotide Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000012465 retentate Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000009105 vegetative growth Effects 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241001566735 Archon Species 0.000 description 1
- 101100316860 Autographa californica nuclear polyhedrosis virus DA18 gene Proteins 0.000 description 1
- 241000244160 Echinococcus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108010044467 Isoenzymes Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000592342 Tracheophyta Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000001261 affinity purification Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 101150039027 ampH gene Proteins 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000000185 dioxinlike effect Effects 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000012456 homogeneous solution Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 239000013618 particulate matter Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920001084 poly(chloroprene) Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- JLEXUIVKURIPFI-UHFFFAOYSA-N tris phosphate Chemical compound OP(O)(O)=O.OCC(N)(CO)CO JLEXUIVKURIPFI-UHFFFAOYSA-N 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Paper (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13701799P | 1999-06-01 | 1999-06-01 | |
| US60/137,017 | 1999-06-01 | ||
| PCT/US2000/015168 WO2000073426A1 (en) | 1999-06-01 | 2000-06-01 | Isolation and activities of lignin depolymerases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2003505060A true JP2003505060A (ja) | 2003-02-12 |
Family
ID=22475448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001512315A Withdrawn JP2003505060A (ja) | 1999-06-01 | 2000-06-01 | リグニンデポリメラーゼの単離および活性 |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP1183334A1 (es) |
| JP (1) | JP2003505060A (es) |
| AP (1) | AP2001002363A0 (es) |
| AU (1) | AU5314600A (es) |
| BR (1) | BR0011528A (es) |
| CA (1) | CA2375030A1 (es) |
| EA (1) | EA200101274A1 (es) |
| IL (1) | IL146830A0 (es) |
| MX (1) | MXPA01012441A (es) |
| NO (1) | NO20015851L (es) |
| PL (1) | PL352317A1 (es) |
| WO (1) | WO2000073426A1 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9796993B2 (en) | 2011-12-12 | 2017-10-24 | Regents Of The University Of Minnesota | Lignin-degrading methods |
-
2000
- 2000-06-01 PL PL00352317A patent/PL352317A1/xx unknown
- 2000-06-01 JP JP2001512315A patent/JP2003505060A/ja not_active Withdrawn
- 2000-06-01 AP APAP/P/2001/002363A patent/AP2001002363A0/en unknown
- 2000-06-01 EA EA200101274A patent/EA200101274A1/ru unknown
- 2000-06-01 MX MXPA01012441A patent/MXPA01012441A/es unknown
- 2000-06-01 AU AU53146/00A patent/AU5314600A/en not_active Abandoned
- 2000-06-01 CA CA002375030A patent/CA2375030A1/en not_active Abandoned
- 2000-06-01 EP EP00938056A patent/EP1183334A1/en not_active Withdrawn
- 2000-06-01 WO PCT/US2000/015168 patent/WO2000073426A1/en not_active Application Discontinuation
- 2000-06-01 IL IL14683000A patent/IL146830A0/xx unknown
- 2000-06-01 BR BR0011528-2A patent/BR0011528A/pt not_active Application Discontinuation
-
2001
- 2001-11-30 NO NO20015851A patent/NO20015851L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| BR0011528A (pt) | 2002-09-17 |
| NO20015851L (no) | 2002-01-14 |
| EP1183334A1 (en) | 2002-03-06 |
| NO20015851D0 (no) | 2001-11-30 |
| CA2375030A1 (en) | 2000-12-07 |
| WO2000073426A1 (en) | 2000-12-07 |
| AU5314600A (en) | 2000-12-18 |
| EA200101274A1 (ru) | 2002-04-25 |
| MXPA01012441A (es) | 2002-11-07 |
| AP2001002363A0 (en) | 2001-12-31 |
| PL352317A1 (en) | 2003-08-11 |
| IL146830A0 (en) | 2002-07-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Application deemed to be withdrawn because no request for examination was validly filed |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20070807 |