JP2003321352A - Composition containing coenzyme q10 - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a composition containing coenzyme Q<SB>10</SB>, which can be dispersed stably in water without generating separation, precipitation, agglomeration and crystallization and also can be absorbed in organism well, while the conenzyme Q<SB>10</SB>is inferior in dispersibility in water due to being slightly soluble in water and having high crystallinity and in addition, is inferior in organism absorption. <P>SOLUTION: The composition containing coenzyme Q<SB>10</SB>contains a peptide, which contains at least 15 mass % of L-glutamine and has an average molecular weight of 200 to 100,000, together with the cozenzyme Q<SB>10</SB>. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a composition containing coenzyme Q ₁₀ . More specifically, the present invention provides coenzyme Q ₁₀ which is poorly dispersible in water due to its poor solubility in water and high crystallinity, and further poor absorbability by living organisms.
Coenzyme Q ₁₀ can be stably dispersed in water without causing separation, sedimentation, aggregation, crystallization, etc.
The present invention relates to a coenzyme Q ₁₀ -containing composition having improved bioavailability.

[0002]

2. Description of the Related Art Coenzyme Q ₁₀ (molecular formula C ₅₉ H
₉₀ O ₄ , molecular weight 863.36, melting point 40 ° C.) belongs to ubiquinones, and length of isoprene unit of side chain of 2,3-dimethoxy-5-methyl-polyprenyl-1,4-benzoquinone (number of repeating units) ) A compound in which n is 10. Coenzyme Q ₁₀ is also called ubidecarenone or coenzyme Q _{10 and} is described in the Japanese Pharmacopoeia. Coenzyme Q ₁₀ is a highly safe compound existing in the living body, and is said to be an essential component for producing adenosine triphosphate in mitochondria as a coenzyme. Coenzyme Q ₁₀ is excellent in safety, has high bioactivity, and improves the immune function of the body to improve heart disease, hypertension,
It is known to be effective for rheumatic valve diseases and the like. Therefore, coenzyme Q ₁₀ is used for applications such as foods and drinks, pharmaceuticals, quasi drugs, feeds, and cosmetics by taking advantage of the above-mentioned characteristics.

However, since coenzyme Q ₁₀ is poorly soluble in water and has high crystallinity, it is difficult to prepare an emulsified composition. When emulsified in water in some way, Coenzyme Q
Crystallization of ₁₀ occurs and separation from the emulsified composition, aggregation and the like easily occur. In order to use Coenzyme Q ₁₀ in medicines, foods and drinks, in order to enhance its effect, Coenzyme Q 10
It is necessary to contain ₁₀ in high concentration, but coenzyme Q
_{Due to} the above-mentioned physical properties such as poor solubility in water and high crystallinity of ₁₀ ,
It has heretofore been difficult to prepare a drug, food or drink, etc. containing coenzyme Q ₁₀ at a high concentration and in a stable state. In addition, since coenzyme Q ₁₀ is fat-soluble, has a large molecular weight, and is highly crystalline, it is poorly absorbed by the body when it is taken orally or enterally, and its excellent physical properties are effectively exhibited in the body. It has the drawback of being difficult to use.

[0004]

The object of the present invention is to provide a measure for stably dispersing coenzyme Q ₁₀ in water or other aqueous liquids without causing separation, aggregation, crystallization, or the like. It is to be. Furthermore, an object of the present invention is to provide a measure for improving the absorbability of coenzyme Q _{10 in} a living body.

[0005]

Means for Solving the Problems The present inventors have made various studies in order to achieve the above object. As a result, with respect to coenzyme Q _10, a particular peptide, that is, L- glutamine content of at least 15% by weight, the average molecular weight is used in combination peptides in the range of 200 to 100,000, of coenzyme Q ₁₀ It has been found that the dispersibility in an aqueous liquid can be improved, whereby separation, precipitation, aggregation, crystallization and the like of coenzyme Q _{10 from} the aqueous dispersion can be prevented. Furthermore, the present inventors have found that when used in combination certain peptides above for coenzyme Q _10, to improve the bioabsorbable coenzyme Q _10, to effectively exhibit excellent functions possessed by Coenzyme Q ₁₀ in vivo It was found that it was possible, and the present invention was completed based on those findings.

That is, the present invention relates to (1) coenzyme Q ₁₀ together with an L-glutamine content of at least 15
Coenzyme Q ₁₀ containing a peptide having an average molecular weight of 200 to 100,000 in mass%
It is a containing composition.

Further, the present invention provides (2) that the content ratio of coenzyme Q ₁₀ and the peptide is coenzyme Q
₁₀ : the peptide = 1: 1 to 1: 1,000 (mass ratio)
A composition containing coenzyme Q ₁₀ according to (1) above, which is:
And (3) the coenzyme Q _10- containing composition according to (1) or (2) above, which is a composition for foods and drinks, pharmaceuticals, quasi drugs, feeds or cosmetics.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. In the composition containing coenzyme Q _{10 of the} present invention (hereinafter sometimes simply referred to as “composition”), coenzyme Q ₁₀
As the above, any of those conventionally known can be used, and the manufacturing method, origin, acquisition method, etc. are not limited at all. And in the present invention, together with coenzyme Q ₁₀ ,
It is necessary to use a peptide having an L-glutamine content of at least 15% by mass and an average molecular weight of 200 to 100,000 (hereinafter, this may be referred to as “glutamine peptide”). In the present specification, the terms "peptide" and "glutamine peptide" mean a peptide composed of L-amino acids that form a natural protein. Thus, the term "L-amino acid" or "amino acid" means the L-amino acids that make up the natural protein.

The glutamine peptide used in the present invention is not particularly limited as long as it has a L-glutamine content of at least 15% by mass and an average molecular weight of 200 to 100,000, and is a partial hydrolyzate of a natural or recombinant protein. , Chemically synthesized or genetically engineered peptides, or a combination thereof. The L-glutamine content in the glutamine peptide is 15
If it is less than mass%, the dispersion stability of coenzyme Q _{10 in} an aqueous liquid, the ability to prevent crystal precipitation, and the bioabsorbability will be low. The upper limit of the L-glutamine content is not particularly limited, but when it exceeds 60% by mass, it is difficult to prepare it from a natural protein, and therefore it is preferably 60% by mass from the viewpoint of availability, preparation, and economy. It is more preferably 20 to 40% by mass. The L-glutamine content in the glutamine peptide is calculated based on the amide nitrogen-containing L-amino acid content obtained from the amide nitrogen content measured by the amide nitrogen substitution method, or when the glutamine peptide is a synthetic product. Can be determined from the ratio of L-glutamine used during synthesis and the like.

When the average molecular weight of the glutamine peptide is less than 200, the dispersion stability of coenzyme Q _{10 in} an aqueous liquid, the ability to prevent crystal precipitation, and the bioabsorbability are low, and moreover, the bitterness is poor and the taste is poor. Become. On the other hand, when the average molecular weight of the glutamine peptide exceeds 100,000,
Dispersion stability of coenzyme Q _{10 in} an aqueous liquid, crystal precipitation preventing property and bioabsorbability are lowered, and a viscous mass is formed when water is added to the glutamine peptide, resulting in poor handleability. The average molecular weight of glutamine peptide is
It is preferably 500 to 20,000, and 1.00
It is more preferably 0 to 10,000. The average molecular weight of the glutamine peptide as used herein is the average molecular weight measured by the gel filtration method, and details thereof are as described in the section of Examples below.

The glutamine peptide used in the present invention is excellent in safety for humans and animals because it is composed of L-amino acids constituting a natural protein. The glutamine peptide used in the present invention contains L-glutamine other than L-glutamine as long as the L-glutamine content is at least 15% by mass.
-The kind of amino acid and its composition ratio are not particularly limited.

The glutamine peptide used in the present invention is a glutamine peptide obtained by hydrolyzing a protein contained in food, particularly a glutamine peptide obtained by hydrolyzing wheat gluten. It is preferable in terms of easiness and economy.

The glutamine peptide preferably used in the present invention is wheat gluten obtained by using a proteolytic enzyme (protease), acid, alkali, etc. and having an average molecular weight of 20.
It can be produced by hydrolysis under conditions such that a hydrolyzate in the range of 0 to 100,000 is produced. Wheat gluten is usually 25 to 50 mass% L
-It contains glutamine and therefore has an average molecular weight of 2
The wheat gluten is hydrolyzed so that it falls within the range of 100 to 100,000, and fractionated as necessary to compare glutamine peptides having L-glutamine contents within the range of 15 to 60% by mass. It can be easily obtained. Wheat gluten is insoluble in water and inferior in dispersibility in water, but glutamine peptide obtained by hydrolyzing wheat gluten is excellent in dispersibility in water because it has a low molecular weight.

The glutamine peptide derived from wheat gluten, which is preferably used in the present invention, although not limited thereto, is described in, for example, JP-A-1-47353 and JP-2985193. It can be produced by a method of hydrolyzing wheat gluten using a protease or a protease and an amylase. In this case, various proteases such as pepsin, trypsin, chymotrypsin, oyster mushroom-derived acidic protease, Aspergillus-derived protease, papain, bromelain, etc. can be used. When hydrolyzing wheat gluten with protease, if amylase is used together, impurities such as starch and fiber contained in wheat gluten are decomposed and removed, and high-purity glutamine peptide can be obtained in high yield. Possible (Patent No. 298)
5193).

The glutamine peptide used in the present invention, for example, the glutamine peptide derived from wheat gluten, has already been commercially available (for example, "Glutamine peptide GP-1" and "Glutamine peptide GP-2" manufactured by Nisshin Pharma Co., Ltd.). Manufactured by DMD, "Glutamine Peptide GP
A "). These glutamine peptides may be used as they are, or may be subjected to treatment such as hydrolysis and fractionation as the case may be.

Coenzyme Q ₁₀ in the composition of the present invention
The content ratio of the glutamine peptide and the glutamine peptide may differ depending on the type of composition, property (phase form), application, usage form, etc., but generally, the ratio of coenzyme Q ₁₀ : glutamine peptide is a mass ratio, The ratio is preferably 1: 1 to 1: 1,000, more preferably 1:20 to 1: 500. If the proportion of glutamine peptide is less than 1-fold by mass of coenzyme Q _10, separated coenzyme Q ₁₀ in an aqueous liquid, aggregation, solidification, tends to become difficult to stably disperse while preventing crystallization, and the like also coenzyme It becomes difficult to obtain the effect of improving the bioabsorbability of Q ₁₀ . On the other hand, when the ratio of the glutamine peptide is more than 1,000 times the mass of coenzyme Q _10, it tends to be excessive doses of the composition when administering the required amount of coenzyme Q _10.

The phase form of the composition of the present invention is not particularly limited, and examples thereof include powder mixture, tablet composition, pill composition, granular composition, aqueous dispersion, liquid edible composition, paste. It may be a solid composition, a wet solid composition, or the like. Further, the type and use of the composition of the present invention is not particularly limited, for example, food and drink (for example, carbohydrate-based foods such as bread and cookies, teas, soups, sprinkles, spreads such as butter and jam, liquid foods). , Juices, jelly drinks, etc., pharmaceuticals (eg tablets, powders,
It can be effectively used for various purposes such as liquids, capsules, granules), quasi-drugs (eg drinks), feed (animal feed, fish feed etc.) and cosmetics (eg emulsion, cream etc.). .

Coenzyme Q ₁₀ in the composition of the present invention
The content of the glutamine peptide and the content of the glutamine peptide can be appropriately adjusted according to each application, purpose and the like. In addition, the composition of the present invention may further contain other components depending on the type and application.

Since the composition of the present invention contains the glutamine peptide together with the coenzyme Q ₁₀ , the poorly water-soluble coenzyme Q _{10 which} is easily crystallized can be stably dispersed in an aqueous liquid to form an aqueous dispersion. Separation, sedimentation, aggregation and precipitation due to crystallization of coenzyme Q ₁₀ from the liquid are prevented. Moreover, by using a glutamine peptide in combination with coenzyme Q ₁₀ , coenzyme Q ₁₀
The bioabsorbability of ₁₀ is improved, and the excellent function of coenzyme Q ₁₀ is effectively exhibited in the body. In addition, the glutamine peptide itself used for stabilizing dispersion and improving bioabsorption of coenzyme Q ₁₀ has a low molecular weight and functions as a nutritional component for an amino acid source with improved digestibility, and further It also works as a liver function improver and immune function enhancer. Therefore, coenzyme Q
The composition of the present invention containing the glutamine peptide together with ₁₀ has various excellent effects as a whole.

[0020]

EXAMPLES The present invention will be described in detail below with reference to examples, but the present invention is not limited to the following examples. The average molecular weight of the glutamine peptide used in the following examples and the glutamine content in the glutamine peptide were measured by the following methods.

Average molecular weight of glutamine peptide: An aqueous solution of glutamine peptide was filtered through a membrane filter having a pore size of 0.45 μm and measured by gel filtration. As a column, "Biosil SEC125-5" manufactured by Japan Bio-Rad Laboratories was used. The measurement conditions are as follows: measurement wavelength 280 nm, eluate 0.2 mM phosphate buffer (pH
6.0, 0.1% SDS) and a flow rate of 0.2 ml / min. As standard molecular weights, ovalbumin (molecular weight 44 kDa), myoglobulin (molecular weight 17 kDa)
Da) and vitamin B12 (molecular weight 1.35 kDa)
It was used.

· Glutamine in glutamine peptide
Amount : (1) Among the 20 kinds of amino acids constituting proteins, the amino acids containing amide nitrogen are two, glutamine and asparagine. When the content ratio (mass ratio) of glutamine (Gln) and asparagine (Asn) in wheat protein was examined by Swiss-Prot which is a protein database, high molecular weight glutenin (ID: G) was obtained.
In LT3_WHEAT, Gln: Asn = 291: 0 (G
ln is 100% by mass, and low molecular weight glutenin (I
D: GLTB_WHEAT), Gln: Asn = 96: 2
(Gln is 98% by mass), and α, β-gliadin (I
D: GAD7_WHEAT), Gln: Asn = 92: 5
(Gln is 95% by mass), and in all wheat proteins, glutamine accounts for 95% by mass or more of the amide nitrogen-containing amino acid contained therein. (2) Therefore, in the following example, the content of the amide nitrogen contained in the glutamine peptide was determined by Wilcox to determine the amount of amide in a chemical substance [Meth. Enzymo
L., 11, 36-65 (1967)]. That is, a glutamine peptide was placed in a flask of Conway, 1N hydrochloric acid was added thereto to liberate the amide nitrogen in the glutamine peptide as ammonia, and the generated ammonia was used as a sanitary inspection guideline [Japanese Society of Pharmaceutical Sciences, “Hygiene Test Method” -Comment ", p274-276, Kanehara Shuppan (1990)], and the amide nitrogen content in the peptide, and thus the amide nitrogen-containing amino acid content, was calculated from the determined ammonia content. (3) As described in (1) above, in wheat protein, among the amide nitrogen-containing amino acids contained therein,
Since the content of asparagine is extremely small, the content of the amide nitrogen-containing amino acid calculated in the above (2) was directly used as the glutamine content in the glutamine peptide.

[0023] "Example 1" [water-dispersible test of coenzyme Q _10] (1) Coenzyme Q ₁₀ (manufactured by Nisshin Pharma Co., Ltd.)
And glutamine peptide (“Glutamine peptide GP-1” manufactured by Nisshin Pharma Co., Ltd .; L-glutamine content 32)
Mass%; average molecular weight 7,000) were mixed well at room temperature in the proportions shown in Table 2 below to prepare powdery mixtures. (2) To 5 g of the powdery mixture obtained in the above (1), 95 ml of distilled water was added, and a homogenizer ("TK homomixer" manufactured by Tokushu Kikagaku Kogyo Co., Ltd.) was used to obtain 8,000.
A dispersion liquid was prepared by stirring at 0 rpm for 20 minutes at 60 ° C., the dispersion liquid was allowed to stand for 30 minutes, and the dispersion state at that time was visually observed according to the evaluation criteria shown in Table 1 below to evaluate the score. The results are shown in Table 2 below. However, in Experiment No. 1, coenzyme Q ₁₀ 5
0 mg was added to 100 ml of water and stirred at room temperature for 20 minutes to prepare a dispersion liquid, and the dispersion state when the dispersion liquid was allowed to stand for 30 minutes was evaluated according to the evaluation criteria shown in Table 1 below.

[0024]

[Table 1]

[0025]

[Table 2]

As can be seen from the results in Table 2 above, in Experiment No. 1 of coenzyme Q ₁₀ alone, the crystals were in a state of floating on the surface of water, and the dispersibility in water was poor. On the other hand, in Experiment Nos. 2 to 7 in which glutamine peptides were combined, the water dispersibility of coenzyme Q ₁₀ was significantly improved, and the precipitation of crystals in water could be prevented.

[0027] [water-dispersible test of coenzyme Q _10] "Example 2" (1) Coenzyme Q ₁₀ (manufactured by Nisshin Pharma Co., Ltd.)
And glutamine peptide (“Glutamine Peptide GP-2” manufactured by Nisshin Pharma Co .; L-glutamine content 31
Mass%; average molecular weight 7,000) were mixed well at room temperature in the proportions shown in Table 3 below to prepare powdery mixtures. (2) 100 g of the powdery mixture obtained in (1) above
Distilled water (900 ml) was added to the mixture, and a homogenizer ("T-K homomixer" manufactured by Tokushu Kikagaku Kogyo Co., Ltd.) was used to stir at 7,000 rpm for 30 minutes at 60 ° C to prepare a dispersion (emulsion) did. However, in Experiment No. 1, 1 g of coenzyme Q ₁₀ was added to 999 ml of distilled water to prepare a dispersion liquid (emulsion) in the same manner as described above. (3) 5 ml of each emulsion obtained in (2) above
Was collected in a test tube (10 ml volume) and the backscattered light intensity was measured with time using a scattered light measuring device (“Turbiscun MA2000” manufactured by Eiko Seiki Co., Ltd.). It was as shown. (4) Further, the state of each emulsion obtained in (2) after 3 hours was visually observed and scored according to the evaluation criteria of Table 1 described above.
It was as shown in.

[0028]

[Table 3]

As can be seen from the results in Table 3 above, in Experiment No. 1 using Coenzyme Q ₁₀ alone, almost all of the irradiation light was scattered backward, and the crystals floated on the surface of water. The dispersibility was poor. On the other hand, in Experiment Nos. 2 to 7 in which coenzyme Q ₁₀ and glutamine peptide were combined, the backscattering intensity of irradiation light was Experiment No. 1
Compared with the above, the water dispersibility of coenzyme Q ₁₀ was greatly improved, and the precipitation of crystals in water was prevented.
Moreover, the increase in backscattered light intensity was extremely small over time, there was no precipitation of coenzyme Q ₁₀ crystals and no emulsion destruction, and the stable emulsified state of coenzyme Q ₁₀ in water was maintained for a long time. .

Example 3 [Coenzyme Q_TenLiving body sucking
Profitability test] (1) Test 1 (control test): (I) Have three healthy men become test subjects (test
Subjects A to C), blood samples from subjects A to C were collected in serum.
Enzyme Q_TenWhen the concentration of was measured, the results are shown in Table 4 below.
It was as shown. The serum used in this Example 3
Coenzyme Q in_TenConcentration is measured by Elund P
HPLC method with O ["J. Chromatogr
a. , 425, 87-97 (1988)].
It was However, oxidized coenzyme Q is present in serum._TenAnd reduction
Type of coenzyme Q_TenIs present in the HPLC sample
Pull using iron (III) chloride dissolved in hydrochloric acid before analysis
Oxidized and all coenzyme Q_TenAs the oxidation type
Was analyzed. (Ii) 60 minutes have passed since the blood was collected in (i) above
At that time, each of the subjects A to C had a rice ball as breakfast.
Have you eat and drink 2 pieces and 1 cup of tea (180 ml)
Then, immediately after that, Coenzyme Q_Ten(Nisshin Pharma Stock
Even if you ingest 100 mg (made by the company) with 180 ml of water
I got it. Curry and rice for subjects A to C 4 hours after breakfast
I had them eat one cup and then took it as free food. That
Ma, Coenzyme Q_Ten2 hours after ingesting
Hours, 8 hours, 12 hours, 24 hours and 48 hours
After a lapse of time, blood samples of subjects A to C were collected and coen in serum was collected.
Zym Q _TenTable 4 below shows the measured concentrations of
It was as it was.

(2) Test 2 (implementation test of the present invention): (i) Two weeks after carrying out the test 1 (control test) of the above (1), blood of the same subjects A to C was sampled in serum. When the concentration of coenzyme Q ₁₀ was measured, it was as shown in Table 4 below. (Ii) At 60 minutes after the blood was collected in the above (i), each of the subjects A to C had two rice balls and a cup of tea (180 ml) as breakfast, and immediately thereafter, 100 mg of the same coenzyme Q ₁₀ as used in (1) and glutamine peptide (“Glutamine peptide GP-1” manufactured by Nisshin Pharma Co., Ltd.) 2
2100 mg of nutritional composition in which 000 mg (2 g) is mixed
Each of the test subjects A to C was ingested together with 180 ml of water. Four hours after breakfast, subjects A to C had one cup of curry rice eaten, and were allowed free intake thereafter.
Meanwhile, blood of subject A to C was collected 2 hours, 4 hours, 8 hours, 12 hours, 24 hours, and 48 hours after the ingestion of coenzyme Q ₁₀ to collect coenzyme Q _{10 in} serum. When the concentration was measured, Table 4 below
It was as shown in.

[0032]

[Table 4]

As can be seen from the results in Table 4 above, subject A
Test 2 in which coenzyme Q ₁₀ was ingested together with the glutamine peptide in all of Examples 2 to C (implementation test of the present invention)
In contrast, the concentration of coenzyme Q _{10 in} serum was higher at an early time point as compared with the test 1 (control test) in which coenzyme Q ₁₀ was taken alone. From these results, in the composition using a combination of glutamine peptide with coenzyme Q _10, it can be seen that improved bioabsorbable coenzyme Q _10.

Example 4 [Preparation of Enteral Nutrition] (1) Of the components shown in Table 5 below, protein (casein), glutamine peptide (“Glutamine peptide GP-1” manufactured by Nisshin Pharma Co., Ltd.) ), Sugars, lipids, minerals, emulsifiers and dietary fiber are mixed in the amounts shown in Table 5 and 500 ml of distilled water is mixed to prepare a mixed solution, and the mixed solution is put into a homomixer for about 9000r.
Coarse emulsification was performed by stirring at pm for 20 minutes. The whole amount of the crude emulsion obtained thereby was transferred to a high pressure emulsifying machine and transferred to 50 MPa.
It was emulsified under the condition of (500 kgf / cm ² ). After cooling the emulsion thus obtained to about 20 ° C., vitamins, flavors and coenzyme Q ₁₀ (manufactured by Nisshin Pharma Co., Ltd.) of the types and amounts shown in Table 5 were added, and then distilled water was added. In addition, the total amount was raised to 1000 ml.

[0035]

[Table 5]

(2) The emulsion obtained in (1) above was transferred to a filling tank and 226 g of each pouch was filled,
Seal the pouch while purging with nitrogen, then 123 ° C
Heat sterilize for 14 minutes with enteral nutrition (concentrated liquid food)
Was prepared. The obtained enteral nutrition is 1 per 100 ml
It has an energy of 00 kcal. (3) As a result of analyzing the enteral nutritional product obtained in (2) above, the content of coenzyme Q ₁₀ was 5 mg / 100 ml,
The pH was 7.01, the average particle size of the particles dispersed in the emulsion was 0.69 μm, and the osmotic pressure was 432 mOsm. Also,
The enteral nutritional product obtained was a milky white emulsion, had a slightly sweet taste, and was excellent in emulsion stability and taste.

Example 5 [Preparation of composition for sports drink] (1) Of the components shown in Table 6 below, all the components except 90% ethanol were mixed, and then 22 parts of 90% ethanol were added thereto. And kneading to prepare a paste, which is extruded through a 16-mesh sieve, granulated, dried at 60 ° C., and used for sports drinks in the form of granules containing coenzyme Q ₁₀ and glutamine peptide. A composition was prepared.

[0038]

[Table 6]

(2) When 20 g of the granular composition for sports drink obtained in (1) above was added to 200 ml of cold water and gently stirred, it was uniformly dissolved and a sports drink was obtained. In the sports drink thus obtained, separation of coenzyme Q ₁₀ was not observed, and it had a uniform liquid state and had a good taste and flavor.

Example 6 [Preparation of composition for miso soup] (1) Of the components shown in Table 7 below, all components (coenzyme Q ₁₀ , glutamine peptide and powdered miso) except 90% ethanol were mixed. After that, 20 parts of 90% ethanol was added thereto and kneaded to prepare a paste-like material, and the paste-like material was extruded from a 16-mesh sieve,
After granulating and drying at 60 ° C., a granular miso soup composition containing coenzyme Q ₁₀ and glutamine peptide was prepared.

[0041]

[Table 7]

(2) 20 g of the granular miso soup composition obtained in (1) above was placed in a bowl, 2 g of dried tofu and 0.5 g of dried chopped green onion were added, and hot water was poured and gently stirred. The composition for miso soup was uniformly dissolved, separation of coenzyme Q ₁₀ was not observed, and the composition was a uniform liquid.
The miso soup thus obtained had good taste and flavor.

Example 7 [Production of noodles] (1) Coenzyme Q ₁₀ (manufactured by Nisshin Pharma Co., Ltd.)
A coenzyme Q ₁₀ composition was prepared by thoroughly mixing 2 g and 20 g of glutamine peptide (“Glutamine peptide GP-1” manufactured by Nisshin Pharma Co., Ltd.). (2) To 400 g of wheat flour (medium-strength flour), 1 g of the coenzyme Q ₁₀ composition prepared in (1) above and 20 g of salt dissolved in 200 g of water were added and mixed, and kneaded well to form a dough. The dough was aged at room temperature for 1 hour. (3) Raw dough was manufactured by rolling the dough laid in (2) above to a thickness of about 5 mm and cutting it into lines of about 5 mm in width. (4) 120 g of the raw udon obtained in (3) above was boiled in boiling water for 10 minutes and put in a soup stock to make udon noodles. At that time, there is no floating, dispersion or separation of the crystals of coenzyme Q ₁₀ on the water in the boiling pot, the surface of the soup of the udon noodles and the surface of the udon, and the appearance is excellent, and the taste and texture are good. there were.

[0044]

The composition of the present invention is a coenzyme Q._TenWhen
Because both contain glutamine peptides,
Zym Q_TenImproves the dispersibility of the
Im Q _TenSeparation, settling, flocculation, crystallization from aqueous dispersion of
Are prevented, and coenzyme Q_TenStable in aqueous liquid
Are dispersed in. Moreover, coenzyme Q_TenAgainst
The composition of the present invention comprising a combination of lutamin peptides,
Enzyme Q_TenHas improved bioabsorption of
Im Q_TenEffectively exhibiting the excellent functions of
You can Further used in the composition of the present invention
Glutamine peptides have a low molecular weight and excel in digestibility.
Functions as a nutritional ingredient for amino acid source
In addition, even if it is a liver function improving agent, immune function activating agent, etc.
Function. Therefore, coenzyme Q_TenWith glutami
The composition of the present invention containing a peptide is generally polar.
It has various excellent effects.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 47/42 A61K 47/42 A61P 9/00 A61P 9/00 9/12 9/12 29/00 101 29 / 00 101 F term (reference) 4B018 LB08 MD07 ME02 4C076 BB01 CC07 EE41 FF33 4C083 AC491 AC492 AD411 AD412 EE03 EE12 4C206 AA01 AA02 CB27 MA01 MA04 MA72 NA11 ZA36 ZA42 ZB15

Claims (3)

[Claims] 1. Coenzyme Q ₁₀ together with an L-glutamine content of at least 15% by weight and an average molecular weight of 20.
A coenzyme Q ₁₀ -containing composition, which comprises a peptide of 0 to 100,000. 2. The content ratio of coenzyme Q ₁₀ and the peptide is coenzyme Q ₁₀ : the peptide = 1: 1.
The coenzyme Q _10- containing composition according to claim 1, wherein the coenzyme Q ₁₀ composition is about 1: 1,000 (mass ratio). 3. The coenzyme Q _10- containing composition according to claim 1 or 2, which is a composition for foods and drinks, pharmaceuticals, quasi drugs, feeds or cosmetics.