JP2003169630A - Nutritional composition containing coenzyme q10 - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a nutritional composition which can effectively supply coenzyme Q<SB>10</SB>, improves the water dispersibility and in vivo absorbability of the coenzyme Q<SB>10</SB>, and has high safety, and to provide a food containing the nutritional composition. <P>SOLUTION: This nutritional composition is characterized by containing the coenzyme Q<SB>10</SB>, sodium caseinate, and dextrin as nutrients. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention contains coenzyme Q ₁₀ (hereinafter referred to as CoQ ₁₀ ) as a nutrient,
And dispersibility of CoQ ₁₀ in water without using a synthetic emulsifier,
The present invention relates to a nutritional composition having excellent bioabsorbability and high safety. More specifically, the present invention relates to a nutritional composition containing CoQ ₁₀ , sodium caseinate and dextrin as nutrients and capable of supplementing CoQ ₁₀ which may be deficient due to aging and the like, and a food containing the same.

[0002]

Coenzyme Q ₁₀ has the following structural formula (I):

[Chemical 1] With the general name ubidecarenone (molecular formula C ₅₉ H ₉₀ O ₄ ,
It has a molecular weight of 863.36) and is almost insoluble in water, but shows high solubility in ether and the like, and is known as a substance that decomposes by light to produce hydroquinone and ubichromenol. CoQ ₁₀ is known as a vitamin-like acting substance having not only biological activity as a coenzyme but also an action of improving oxygen utilization efficiency. In addition, it is a metabolic cardiotonic drug, and its pharmacological effects such as improvement of mild and moderate congestive heart failure symptoms are recognized.

CoQ ₁₀ tends to be deficient due to aging, and it is desirable to supplement CoQ ₁₀ as a nutrient in order to prevent or reduce the symptoms of heart diseases, such as ischemic heart disease, which are common in the elderly. However, although CoQ ₁₀ is generally known to be contained in fish, meat and seaweed,
Very few natural food materials have a high CoQ ₁₀ content. Therefore, it is difficult to supplement the normal diet with an amount of CoQ ₁₀ to compensate for the deficiency from natural food materials.

CoQ ₁₀ is a lipophilic solid with a low melting point,
It is known that it is poorly absorbed in oral administration due to its poor solubility in water. When CoQ _{10 is} to be supplied as a nutrient, it is required to improve its absorbability and prevent crystallization, coagulation and precipitation. In addition, it is important that it is highly safe in consideration of oral intake.

[0005]

DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention
It is an object of the present invention to provide a nutritional composition which can be effectively supplemented with Q ₁₀ , has excellent dispersibility in water, is highly absorbable in vivo, and is highly safe without causing crystallization and aggregation of CoQ ₁₀ .

[0006]

Means for Solving the Problems As a result of intensive studies for solving the above problems, the present inventors have found that CoQ ₁₀ is almost insoluble in water, and is prone to crystallization and aggregation / precipitation. Is unstable, but Co
By including Q ₁₀ together with sodium caseinate (also referred to as casein Na) and dextrin, which are also used in foods, excellent dispersibility in water and bioavailability without using a synthetic emulsifier, and safety It has been found that a nutritional composition having a high value can be obtained. Further, the nutritional composition is C
When administered or ingested to elderly people or fragile people who lack oQ ₁₀ , supplement the necessary nutrients and CoQ ₁₀ ,
It was found that nutrition status could be improved in a short period of time.

Therefore, the present invention uses Co as a nutrient.
A nutritional composition comprising Q ₁₀ , Na casein and dextrin.

[0008] The present invention is, with respect to coenzyme Q ₁₀ 1 part by weight, 25～1,500 parts sodium caseinate relates nutritional compositions containing dextrin 50～6,000 parts by weight, preferably, coenzyme Q ₁₀ It relates to a nutritional composition containing 50 to 1,200 parts by weight of sodium casein and 150 to 4,000 parts by weight of dextrin per 1 part by weight. In the nutritional composition of the present invention,
If the content of casein Na and dextrin is less than the above range, the stability of CoQ ₁₀ is inferior in stability and dispersibility in water, which is not preferable and the bioabsorbability is also low. Also,
If the content of casein Na and dextrin is larger than the above range, the amount of the nutritional composition necessary for ingesting sufficient CoQ ₁₀ will be large, and for the convenience of ingestion by dysphagic persons such as the elderly and the weak, Not preferable.

The present invention also relates to a nutrition containing CoQ ₁₀ , casein Na and dextrin, which further contains proteins other than sodium caseinate, sugars other than dextrin, lipids, dietary fiber, vitamins and / or minerals as other nutritional ingredients. It relates to a composition.

The present invention also relates to a composition in the form of a liquid food which is used by being dissolved or dispersed in an aqueous liquid, and is dissolved or dispersed in 200 to 80,000 parts by weight of an aqueous liquid based on 1 part by weight of Coenzyme Q _10. The present invention relates to a nutritional composition in the form of a liquid food used as described above, wherein the energy per 100 mL of the composition is 60 kcal or more. The invention further relates to food products containing these compositions.

The nutritional composition of the present invention further comprises casein N
Proteins other than a, sugars other than dextrin, lipids, dietary fiber and the like can be contained. in this case,
The weight ratio of sodium casein to total protein ranges from 0.2 to 1, preferably 0.5 to 1. Also,
The weight ratio of dextrin to total sugar is in the range of 0.1 to 1, preferably 0.3 to 1.

Further, the amount of lipid in the nutritional composition is Co
Against Q ₁₀ 1 part by weight, 0 to 8.0 parts by weight, preferably 2.0 to 6.0 parts by weight, the amount of dietary fiber, CoQ ₁₀
It is 0 to 4 parts by weight, preferably 0 to 2 parts by weight, relative to 1 part by weight.

The nutritional composition of the present invention is a liquid food used by dissolving or dispersing 1 part by weight of CoQ _{10 in} 200 to 80,000 parts by weight of an aqueous liquid, preferably a concentrated liquid food with high nutrition and high energy. You can In this case, it may be prepared as a liquid composition from the beginning, but it may be prepared as a powder and dissolved in a predetermined amount of an aqueous liquid before use. The nutritional composition has a protein content of 40 to 70 g / L and a carbohydrate content of 1 L.
Contains 10 to 220 g / L, lipids 0 to 80 g / L, and dietary fiber 0 to 20 g / L, and has an energy of 100
It is 60 kcal or more per mL. Further, various nutrients such as various vitamins and minerals, and stabilizers such as dispersants and emulsifiers, and additives such as sweeteners and flavors can be added. The nutritional composition of the present invention is suitable for use in frail elderly people, dysphagia patients, severely disabled people, patients with heart diseases such as ischemic heart disease, and preoperative and / or postoperative patients.
Necessary nutrition and energy including Q ₁₀ can be surely supplied by the enteral feeding method.

The present invention also relates to a food product containing the nutritional composition, preferably a liquid food product which may be a liquid food. Liquid food may be prepared as a liquid food from the beginning,
It may be prepared as a powder and dissolved in a predetermined amount of an aqueous liquid to be ingested. It is preferable to add various additives such as sweeteners and flavors to the liquid food in order to improve the flavor. INDUSTRIAL APPLICABILITY The food of the present invention is useful for improving the QOL or treating at home, etc. of an elderly person with independence who can eat their own food, a patient with dysphagia or a patient with relatively mild symptoms.

[0015]

BEST MODE FOR CARRYING OUT THE INVENTION Since CoQ ₁₀ is poorly soluble in water, it is usually dissolved in a non-hydrophilic organic solvent such as vegetable oil or animal oil, or it is dissolved in an aqueous solution using a synthetic emulsifier or the like. Used by dispersing and emulsifying. However, in the nutritional composition of the present invention, CoQ ₁₀ only needs to be blended together with casein Na and dextrin, has excellent dispersibility in an aqueous solution such as liquid food, and easily aggregates without crystallizing. Never. In addition, casein is a major protein in milk, accounting for about 80% of milk protein, and casein Na is water-soluble and has functions such as water retention, emulsification, foaming, shape retention, and binding, so that ice cream, bread , Biscuits, ham, sausage, etc. Dextrin is a general term for a mixture of oligosaccharides consisting of glucose, and is obtained by partially hydrolyzing starch. Since these casein Na and dextrin are used for food, they are highly safe.

The content of CoQ ₁₀ in the nutritional composition varies depending on the condition of the person to be ingested, body weight, etc., but is generally 0.0005 to 0.2% by weight, preferably 0.001 to 0.005% by weight.
It is in the range of 1% by weight, and is determined in consideration of the daily dose of CoQ ₁₀ , as well as dispersion stability and flavor. Also, CoQ
The daily dose of ₁₀ is usually in the range of 1 to 300 mg, preferably 10 to 100 mg, though it varies depending on the health condition of the recipient.

When the nutritional composition of the present invention is a nutritional composition in the form of liquid food, proteins other than casein Na, sugars other than dextrin, lipids, plant fibers, vitamins, various minerals, etc. You may contain as a component. The composition and amount of these components are determined by the amount of energy of the liquid food itself, the amount of each nutrient desired, the C content.
Although it can be appropriately determined in consideration of the amount of oQ ₁₀ , etc.,
The amount of energy per 100 mL of liquid food is adjusted to be 60 kcal or more, preferably 100 kcal or more to 230 kcal or less.

In the nutritional composition of the present invention, the use of casein Na alone as the protein is the best in the property stability and dispersibility of CoQ _{10. When} casein salts other than casein Na are used, the property stability and dispersibility are improved. Dispersibility decreases. However, as a protein other than casein Na, for example, animal protein such as casein or casein calcium, milk protein, chicken egg protein, meat protein; vegetable protein such as soybean protein or soybean peptide; only one of these protein degradation products and amino acids. Alternatively, two or more kinds may be used in combination with casein Na. The total amount of protein containing casein Na was 25 to 1,50 with respect to 1 part by weight of CoQ _10.
It is 0 part by weight, preferably 50 to 1,200 parts by weight.
Specifically, the total protein containing casein Na is 5 to 30%, preferably 10 to 30% of the total energy amount of the nutritional composition.
The range is 25%.

In the nutritional composition of the present invention, Co
As the sugar used in combination with Q ₁₀ and casein Na, dextrin, such as maltodextrin and dextrin, is preferable, but as other sugars, powdered candy, glucose,
Only one kind selected from monosaccharides such as fructose, disaccharides such as maltose, lactose and sucrose, oligosaccharides, or two or more kinds thereof may be used in combination with dextrin. The total sugar mass containing dextrin is 50 to 6,000 parts by weight, preferably 150 to 4,000 parts by weight, based on 1 part by weight of CoQ _10.
Parts by weight. Specifically, the total sugar content including dextrin is 10 to 70%, preferably 50 to 70% of the total energy amount of the nutritional composition.

Examples of lipids that can be used include rice bran oil and soybeans.
Oil, cottonseed oil, safflower oil, sunflower oil, corn oil, vegetables
Seed oil, olive oil, palm oil, perilla oil, perilla oil, palm
Vegetable oils such as oil; animal oils such as beef tallow and lard; fish
Oil; medium chain fatty acid triglyceride (MCT);
Contains other synthetic triglycerides, etc.
You may use only one kind or a combination of two or more kinds.
Yes. The lipid content is CoQ _Ten0 to 1 part by weight
8 parts by weight, preferably 2 to 6 parts by weight,
Is 0 to 30% of the total energy of the liquid food, preferably 1
The range is 5 to 25%.

Examples of dietary fibers that can be used include cellulose, lignin, resistant starch, polydextrose, oligosaccharides, indigestible polysaccharides such as guar gum enzymatic degradation products, and the like. Only one of these dietary fibers or a combination of two or more thereof may be used. The content of dietary fiber is 0 to 4 parts by weight, preferably 0 to 2 parts by weight, based on 1 part by weight of CoQ ₁₀ .

Examples of vitamins that can be added include vitamin A, vitamin B ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , vitamin C, vitamin D, vitamin E, vitamin K, niacin, folic acid, and pantothene. Fat-soluble or water-soluble vitamins such as acids are included, and these vitamins may be used alone or in combination of two or more. The amount of each vitamin is determined according to, for example, the intake amount (target intake amount) described in the 80-year-old male activity intensity I described in the 6th revised "Japanese nutritional requirements" (published by the first publisher). can do.

Examples of minerals that can be added include calcium, iron, sodium, zinc, copper, potassium, phosphorus, magnesium, iodine, manganese, selenium, etc., and one or more of these minerals can be used. A combination may be used. The blending amount of these minerals can be determined according to the intake amount (target intake amount) described in the sixth revised “Japanese nutritional requirements”, as described above.

However, deficiency of magnesium also induces neurological diseases, mental disorders, arrhythmias, etc., and calcium can prevent or ameliorate osteoporosis. Therefore, it is preferable to add / compound these mineral components to the nutritional composition. . The amount of calcium is usually about 4 per meal.
In the range of 0 to 1200 mg, magnesium is about 200 to
The range is 600 mg / L. At that time, in order to prevent diseases such as myocardial infarction and ischemic heart disease, it is preferable to mix calcium and magnesium in a weight ratio of Ca: Mg = 2: 1 to 1: 1. If the proportion of magnesium is less than the above range, it may cause various diseases such as ischemic heart disease.

The reason is that magnesium has a function of activating the enzyme ATPase which is an energy source in cells. On the other hand, ATPase activates the intracellular calcium pump, which keeps the intracellular calcium concentration low. Therefore, by adding an appropriate amount of magnesium, intracellular calcium concentration is maintained low by magnesium even in vascular smooth muscle, and as a result, spasm of vascular smooth muscle is prevented from occurring and ischemic heart disease is prevented. It is.

Examples of emulsifiers that can be added to the nutritional composition of the present invention as additives are glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, phospholipid,
It includes gum arabic, xanthan gum, tragacanth gum, guar gum, locust bean gum or mixtures of two or more thereof. Furthermore, for example, flavoring components such as sodium chloride, sodium glutamate, citric acid, sodium citrate, fruit juice, and flavors such as coffee flavor, matcha flavor, and milk flavor can be appropriately added.

The nutritional composition of the present invention is provided mainly in the form of liquid food, but it may be in the form of powder, solid, paste or the like obtained by mixing and drying the above components. In this case, these may be ingested directly or when dissolved or dispersed in an aqueous liquid such as water or warm water at the time of ingestion. The liquid food can be prepared according to a conventional method. For example, the liquid food can be produced by adding the weighed components to water, mixing them sufficiently, emulsifying, filling and sealing in a retort pouch, and performing sterilization treatment.

The liquid food obtained in this manner is sufficient for the frail elderly, dysphagia, preoperative and / or postoperative patients, chronic heart disease patients, chronic respiratory disease patients, etc.
And simultaneously replenish Q _10, can be supplied nutrients and energy required.

The invention is illustrated by the following examples.
The present investigation is not limited to the following examples.
In all the examples, Co manufactured by Nisshin Pharma Co., Ltd.
The Q ₁₀ was used.

Example 1 Preparation of nutritional composition Each component was weighed according to the formulation shown in Table 1. Next, to 200 g of purified water, casein Na (manufactured by DMV) and dextrin (manufactured by Matsutani Chemical Co., Ltd.) were added and mixed to give about 65
It was heated to 0 ° C. and dissolved. 0.2 g of CoQ _{10 in} this solution
Was added and dissolved by stirring with a stirrer (TK homomixer, manufactured by Tokushu Kika Kogyo Co., Ltd.) at about 5000 rpm for 10 minutes to obtain a liquid nutritional composition (liquid food). Then, the liquid food was stored indoors and returned to room temperature. Also, liquid food 10
0 g was dried with a freeze dryer (Central Science & Trade Co., Ltd.) FD-5 type to obtain about 8 g of a powdery nutritional composition.

Examples 2-3 Each component was weighed according to the formulation shown in Table 1. Next, the same operation as in Example 1 was performed to prepare each liquid nutritional composition, and the temperature was returned to room temperature.

Comparative Examples 1 to 5 Each component was weighed according to the formulation shown in Table 1. Next, the same operation as in Example 1 was performed to prepare each liquid nutritional composition.

[0032]

[Table 1]

Comparative test 1. Dispersion stability evaluation test (1) Test method The nutritional compositions of Examples 1 to 3 and Comparative Examples 1 to 5 were each transferred to a transparent glass bottle and capped, and then an autoclave (Autoclave SS-325 manufactured by Tommy Seiko Co., Ltd.).
(Type) and allowed to stand at room temperature, and the precipitation of CoQ ₁₀ crystals was evaluated according to the following evaluation criteria. ◎ No precipitation of CoQ ₁₀ crystals was observed at all for 5 days or more ○ Some CoQ ₁₀ crystals were observed after 5 days or more × CoQ ₁₀ crystals floated immediately (within 12 hours) after standing (2) Test results conducted Example 1 ... ◎ Example 2 ・ ・ ・ ◎ Example 3 ・ ・ ・ ○ Comparative Example 1 ・ ・ ・ × Comparative Example 2 ・ ・ ・ × Comparative Example 3 ・ ・ ・ × Comparative Example 4 ・ ・ ・ × Comparative Example 5 ... × From the above results, it was shown that the stability of CoQ ₁₀ and the dispersibility of CoQ ₁₀ were extremely good by combining CoQ ₁₀ with casein Na and dextrin.
It was shown that when casein Ca was used as a substitute for a, the property stability and dispersibility decreased.

2. Absorption Experiments The nutritional compositions of Example 1 and Comparative Example 3 were evaluated for CoQ ₁₀ absorption by a forced administration experiment using Beagle dogs. (1) Test method The nutritional compositions of Example 1 and Comparative Example 3 were single-forced to each of three (male) beagle dogs using a catheter at a dose of 90 mg / animal each. After administration, blood was collected at regular intervals up to 24 hours after administration, and the time course of CoQ ₁₀ concentration in plasma was examined. C in plasma
The oQ ₁₀ was measured using HPLC under the following conditions. Column: Nucleosil 5C18 4.6mm * 25cm Mobile phase: Ethanol: Acetonitrile (55:45) Flow rate: 1ml / min Detector: Ultraviolet spectrophotometer 275nm

(2) Results Pharmacokinetic parameters of CoQ ₁₀ in plasma, especially AU
When the nutritional compositions of Example 1 and Comparative Example 3 were compared for C and Cmax, the nutritional composition of the present invention showed that CoQ
The bioavailability of ₁₀ showed significant absorbability. The obtained pharmacokinetic parameters are shown in Table 2 below.

[0036]

[Table 2]

[0037]

According to the present invention, excellent dispersion stability and bioabsorbable CoQ _10, nutritional compositions which can replenish CoQ ₁₀ effectively and foods containing the same are provided.
Since the nutritional composition of the present invention is a combination of CoQ ₁₀ with casein Na and dextrin used in foods, it is very safe. Therefore, this nutritional composition provides a safe, reliable, and easy supplementation of a sufficient amount of CoQ ₁₀ and necessary nutrition and energy to bedridden frail elderly people, dysphagia patients, etc. by the enteral nutritional supplementation method and the like. And then C
Can prevent and ameliorate oQ ₁₀ deficiency. In addition, the nutritional composition can be mixed with various foods to supplement nutrients that tend to be insufficient.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 31/122 A61K 31/122 31/718 31/718 38/17 A61P 3/02 A61P 3/02 9 / 04 9/04 9/10 9/10 43/00 111 43/00 111 A61K 37/16 F term (reference) 4B018 MD07 MD21 MD36 4C076 AA12 AA22 BB01 CC11 CC21 CC22 CC40 FF36 FF43 4C084 AA02 AA03 BA05 CA20 CA38 MA17 MA23 MA52 NA03 NA11 ZA372 ZA402 ZC192 ZC212 4C086 AA01 AA02 EA20 MA03 MA04 MA17 MA23 NA03 NA11 ZA37 ZA40 ZC19 ZC21 4C206 AA01 AA02 CB27 KA01 MA03 MA04 MA28 MA37 MA43 MA72 NA03 NA11 ZA37 ZA40 ZC21

Claims (5)

[Claims] 1. A nutritional composition comprising coenzyme Q ₁₀ , sodium caseinate and dextrin as nutrients. 2. The nutritional composition according to claim 1, which contains 25 to 1,500 parts by weight of sodium casein and 50 to 6,000 parts by weight of dextrin per 1 part by weight of coenzyme Q ₁₀ . 3. A protein other than casein sodium,
The nutritional composition according to claim 1 or 2, containing a carbohydrate other than dextrin, a lipid, dietary fiber, vitamins and / or minerals. 4. An aqueous liquid containing 1 part by weight of coenzyme Q ₁₀
A nutritional composition in the form of a liquid food used by being dissolved or dispersed in 200 to 80,000 parts by weight, wherein the energy per 100 mL of the composition is 60 kcal or more. The nutritional composition described. 5. A food containing the nutritional composition according to any one of claims 1 to 4.