JP2003306440A - Skin care preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a skin care preparation which has a high moisture retaining effect and further has skin roughness-improving and skin ageing syndrome (wrinkles or the like)-improving effects which are synergistically improved. <P>SOLUTION: This skin care preparation comprises the extract of a plant belonging to the genus Clintonia in the family Uvulariaceae and one or more conventional anti-inflammatory agents such as glycyrrhizin, glycyrrhetic acid, azulene, salicylic acid, allantoin, their derivatives, their salts, and galenical extracts. Clintonia borealis or Clintonia udensis is suitably used as the plant belonging to the genus Clintonia. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention has a high moisturizing effect,
The present invention relates to a skin external preparation which synergistically improves the effects of improving skin aging symptoms such as wrinkles and the effect of improving rough skin. More particularly, Uburariasea (Uvulariaceae) family clintonia udensis (Clinto
nia ) genus plant extract and one or more selected from anti-inflammatory agents in combination.

[0002]

2. Description of the Related Art Aging, UV exposure, oxidative stress due to reactive oxygen species generated in skin tissue, contact with drugs and various allergens, etc., cause inflammatory reaction of skin and damage and aging of skin. It is known. In the field of external preparations for skin, various physiologically active ingredients such as active oxygen species scavengers, anti-inflammatory agents, anti-allergic agents, and cell activating agents have been searched and studied in order to prevent such skin inflammation and aging. It was In recent years, there has been an increasing tendency to seek such ingredients from plants, reflecting the natural and plant orientations of consumers.

However, among the above-mentioned components of plant origin which have already been reported, since the activity is low, it takes a considerably high concentration or is stable in order to obtain a sufficient action and effect when incorporated into a skin external preparation. All of the stability and safety of the formulation and the action and effect when blended in a skin external preparation, such as those that have problems in terms of safety and safety, and those that impart an undesirable color or odor to the skin external preparation. In the present situation, there are few things that can be satisfied. In addition, since various factors intricately participate in the inflammatory reaction, damage, aging, etc. of the skin, it was not possible to obtain a sufficient effect even if a substance acting only in a part of the above reaction was used. .

[0004]

Therefore, in the present invention, by sufficiently improving the physiological functions of the skin, the moisturizing effect is high, and the effect of improving skin aging symptoms such as wrinkles and the effect of improving rough skin are synergistic. The object was to obtain an improved external preparation for skin.

[0005]

Made various studies to solve the problems mentioned earlier, there is provided a means for solving], Uburariasea (Uvularia
ceae ) family swallow moto ( Clintonia ) genus plant extract,
Included in skin external preparations in combination with one or more selected from commonly used anti-inflammatory agents such as glycyrrhizinic acid, glycyrrhetinic acid, azulene, salicylic acid, allantoin and their derivatives, their salts, and herbal extracts. It was found that by doing so, an excellent moisturizing effect can be obtained, and a synergistic improvement of the skin aging symptoms such as wrinkles and the skin roughening improvement effect can be obtained, and the present invention has been completed.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION First, an extract of a plant of the genus Clin tonia of the family Uvulariaceae used in the present invention will be described.

[0007] The vulvaraceae family ( Uvulariaceae ) used to obtain the external preparation for skin according to the present invention ( Clin
Tonia ) plants are sometimes classified in the Liliaceae family and are perennial plants that are isolated and distributed in North America and East Asia. C. andrewsia
na ), Clintonia borealis ( C. borealis ), Clintonia udensis ( C. udensis ), Clintonia umbellulata ( C. umbellulata ), Clintonia uniflora ( C. uniflora ) are known and available. For ease of use, C. borealis ( C. borealis )
s ), Clintonia udensis ( C. udensis ) can be preferably used. For extraction, flowers, fruits, stems, leaves,
Use one or more species selected from each part such as roots, or whole grass.

In the present invention, Ubularia sea ( Uvul
The genus ariaceae ) swallow moto ( Clintonia ) genus plant is raw or after being subjected to treatments such as shredding, drying and crushing,
Extraction is performed by immersing in an extraction solvent. Stirring may be performed to improve the extraction efficiency, or homogenization may be performed in the extraction solvent. As the extraction temperature, it is appropriate to set the temperature to about 5 ° C. to a temperature not higher than the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is suitable to be about 4 hours to 2 weeks. Alternatively, an extraction method using a supercritical fluid or a subcritical fluid can be used.

It is most preferable to use water as the extraction solvent, but in addition to this, lower alcohols such as methanol, ethanol, propanol and isopropanol, 1,
Polyhydric alcohols such as 3-butylene glycol, propylene glycol, dipropylene glycol and glycerin,
Ethers such as diethyl ether and dipropyl ether, esters such as ethyl acetate and butyl acetate, polar organic solvents such as ketones such as acetone and ethyl methyl ketone, physiological saline, phosphate buffer, phosphate buffer Physiological saline, petroleum ether, n-hexane, n-
One or two selected from hydrocarbons such as pentane, n-butane, n-octane and cyclohexane, non-polar or low polar solvents such as carbon tetrachloride, chloroform, dichloromethane, trichloroethylene, benzene and toluene.
More than one solvent can be preferably used. Further, a supercritical fluid or a subcritical fluid such as water, carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia or the like may be used for the extraction, and in this case, the above-mentioned solvent may be used as an entrainer.

The extract can be added as it is to the base of the external preparation, but the concentrated and dried product can be redissolved in water or a polar solvent, or can be purified or fractionated by decolorization, deodorization, desalting, etc. You may use it after performing a process. For storage, it is preferable to freeze-dry after purification and dissolve in a solvent before use. Alternatively, they can be encapsulated in vesicles such as liposomes or microcapsules.

[0011] The amount of the extract of the plant of the genus Clintonia of the family Uvulariaceae belonging to the skin external preparation is not particularly limited, but its effect does not change even if it is added in a large amount, and .0001-10
It is preferably in the range of 0.001 to 5% by weight, and more preferably 0.001 to 5% by weight.

In the present invention, the above-mentioned plant extract of the genus Clintonia of the family Uvulariaceae is used in combination with one or more kinds selected from anti-inflammatory agents in the base of the external skin preparation. .

Examples of such anti-inflammatory agents include cortisone, hydrocortisone, prednisolone, methylprednisolone, dexamethasone, betamethasone, triamcinolone, triamcinolone acetonide, fluocinolone acetonide, fluocinonide, beclomethasone and their phosphates, propionates, acetates. Steroidal anti-inflammatory agents such as salts and succinates, salicylic acid derivatives such as salicylic acid and aspirin, salicylamide, etenzamid, methyl salicylate, indoleacetic acid derivatives such as indomethacin and sulindac, phenylbutazone, oxyphenbutazone and other pyrazos Lysinedione derivatives, anthranilic acid derivatives such as mefenamic acid and flufenamic acid, propionic acid derivatives such as ibuprofen, ketoprofen and naproxen, diclofena Non-steroidal anti-inflammatory agents such as phenylacetic acid derivatives such as ku, fenbufen, benzothiazine derivatives such as piroxicam, salts and derivatives of glycyrrhizinic acid and dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, stearyl glycyrrhetinate and glycyrrhetinyl stearate, glycyrrhetinyl stearate, Salts and derivatives of 3-succinyloxyglycyrrhetinic acid disodium, guaiazulene, ethyl guaiazulene sulfonate, sodium guaiazulene sulfonate, azulene derivatives such as camazulene, allantoin, aloin, aloe-emodin, shikonin and isobutylshikonin, acetylshikonin, isovalerylshikonin Such as ginsenoside R _a1 , ginsenoside R _a2 , ginsenoside R _b1, etc., and 2 Examples thereof include ginsenoside derivatives such as 0-glucoginsenoside R _f , paeoniflorin, paeonol and paeonol derivatives such as paeonoside and paeonolide.

[0014] as anti-inflammatory agents in the present invention, lithospermum (Lithospermi Radix), carrots (Ginseng Radi
x ), Peonies ( Paeoniae Radix ), Button pie ( Mouta
n Cortex ), kujin ( Sophorae Radix ) and the like, and herbal extracts used as anti-inflammatory agents can also be used.

In the present invention, one of the above anti-inflammatory agents is used.
The seeds may be used alone or in combination of two or more. The amount of these anti-inflammatory agents added to the external preparation for skin depends on the anti-inflammatory properties of each anti-inflammatory agent, but is generally 0.0001 to 5% by weight, preferably 0.001 to 5% by weight. Appropriate.

The external preparation for skin according to the present invention can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders and granules. In addition, skin cosmetics such as lotion, emulsion, cream, beauty essence, pack,
Makeup base lotion, base cosmetics such as makeup base cream, foundation of each dosage form such as milky liquid, oily, solid, etc., makeup cosmetics such as eye color, cheek color, cleansing cream, cleansing lotion, cleansing foam , It can also be provided as a skin cleansing agent such as face wash soap and body shampoo, and a hair cosmetic such as hair shampoo, hair rinse and hair treatment.

The external preparation for skin according to the present invention includes, for example, Clintoni of the family Uvulariaceae.
a ) In addition to genus plant extracts and anti-inflammatory agents, general pharmaceuticals and cosmetics such as oily ingredients, surfactants, humectants, pigments, UV absorbers, antioxidants, fragrances, antibacterial and antifungal agents, etc. Raw materials and physiologically active ingredients such as cell activating agents and whitening agents can be contained.

[0018]

EXAMPLES The features of the present invention will be described in detail with reference to Examples.

First, the Ubularia sea ( Uv used in the present invention
An extract of the genus Clintonia of the ulariaceae family was prepared in the following manner.

Extract of plant of genus Swallow-tailed moto 1 After crushing Clintonia borearis, 500 g was immersed in 2,000 mL of purified water, extracted by stirring at 25 ° C. for 7 days, and then filtered to collect a filtrate. It was designated as Plant Extract 1.

Swallow's moto plant extract 2 After crushing Clintonia borealis, 500 g of 5
Immerse in 1000 mL of 0% by volume ethanol aqueous solution, and
It was left still at 7 ° C for 7 days. After that, the insoluble matter was removed by filtration, the filtrate was concentrated under reduced pressure, and further freeze-dried to obtain a swallow moto plant extract 2.

Swallow's moto plant extract 3 After crushing Clintonia udensis, 500 g of
0% by volume 1,3-butylene glycol aqueous solution 2.00
The extract was immersed in 0 mL, stirred and extracted at 20 ° C. for 5 days, and then filtered to collect a filtrate, which was designated as an extract 3 of the genus Swallowweed.

Swallow's moto plant extract 4 After crushing Clintonia udensis, 50 g of 40
The mixture was extracted with a supercritical carbon dioxide fluid of 15 MPa and 0.003% ethanol as an entrainer at 0 ° C. for 2 hours, and the obtained crude product was designated as a swallow moto plant extract 4.

Next, the formulations of the examples of the external preparation for skin according to the present invention will be shown. Unless otherwise specified, the unit weights in the examples are shown by weight%.

[Examples 1 and 2] Lotion (1) Ethanol 20.00 (2) Polyoxyethylene (60 EO) hydrogenated castor oil 1.00 (3) Swallow moto plant extract 20. 10 (4) Anti-inflammatory agent shown in Table 1 0.10 (5) Methyl paraoxybenzoate 0.10 (6) Dipropylene glycol 5.00 (7) 1,3-Butylene glycol 10.00 (8) Purified water Amount in which the total amount is 100: Manufacturing method: (2) and (3) are added to (1) and dissolved to obtain an alcohol phase. On the other hand, (4) to (7) are sequentially dissolved in (8) to form an aqueous phase. The alcohol phase is added to the aqueous phase, and the mixture is stirred and mixed. Incidentally, moutan bark extract, moutan bark (Mo
Utan Cortex ) (200 g) was dried, pulverized, immersed in 1 liter of 50% by volume ethanol aqueous solution, allowed to stand at 25 ° C. for 7 days, and then filtered to obtain a filtrate.

[0026]

[Table 1]

[Examples 3 and 4] Emulsion (1) Cetanol 1.00 (2) Beeswax 0.50 (3) Vaseline 2.00 (4) Squalane 6.00 (5) Dimethylpolysiloxane 2.00 (6) Polyoxyethylene (20 EO) sorbitan monostearate 1.00 (7) Glycerin monostearate 1.00 (8) Glycerin 4.00 (9) 1,3-Butylene glycol 4.00 (10) Methyl paraoxybenzoate 0.10 (11) Purified water Amount based on total amount of 100 (12) Carboxyvinyl polymer (1.0% aqueous solution) 10.00 (13) Potassium hydroxide (10.0% aqueous solution) 1.00 (14) Anti-inflammatory agent shown in Table 2 0.10 (15) Swallow-tailed moto plant extract 1 0.02 Production method: Oil phase composition of (1) to (7) Were mixed, and heated and dissolved to 75 ° C.. On the other hand, the aqueous phase components (8) to (11) are mixed and dissolved to 75 ° C. After the above oil phase was added to this and pre-emulsified, (12) was added to uniformly emulsify with a homomixer, then (13) was added to thicken and then cooled, and the mixture was cooled at 40 ° C. (14). , (15) are added and mixed. The carrot extract is a carrot ( Ginseng Rade
ix ) 225 g in 1.2 liters of warm water at 50 ° C.
The mixture was extracted with stirring for an hour, filtered, and the filtrate was recovered.

[0028]

[Table 2]

[Examples 5 and 6] Oil-in-water cream (1) Beeswax 6.00 (2) Cetanol 5.00 (3) Reduced lanolin 8.00 (4) Squalane 27.50 (5) Glycerin Fatty acid ester 4.00 (6) Lipophilic glycerin monostearate 2.00 (7) Polyoxyethylene (20 EO) sorbitan monolaurate 5.00 (8) Anti-inflammatory agent shown in Table 3. 10 (9) Propylene glycol 5.00 (10) Methyl paraoxybenzoate 0.10 (11) Swallowtail moto plant extract 4 0.02 (12) Purified water Amount based on 100 total amount Manufacturing method: (1) to (8) 75) by mixing and dissolving the oil phase components
℃. On the other hand, by dissolving (10) in (9), (11)
Both are added to (12), mixed and dissolved, and heated to 75 ° C. Next, the oil phase component is added to this aqueous phase component to pre-emulsify it, then homogenize with a homomixer and cool. In addition, Sikon extract is Likon ( Lithospermi Radi
x ) 200 g was dried, pulverized, immersed in 1 liter of 50% by volume aqueous ethanol, allowed to stand at 25 ° C. for 7 days, and then filtered to obtain a filtrate.

[0030]

[Table 3]

[Examples 7 and 8] Gel agent (1) Dipropylene glycol 10.00 (2) Carboxyvinyl polymer 0.50 (3) Potassium hydroxide (10.0% aqueous solution) 1.00 (4 ) Methyl paraoxybenzoate 0.10 (5) Swallow's moto plant extract 3 0.10 (6) Anti-inflammatory agent shown in Table 4 0.10 (7) Purified water Amount based on 100 Manufacturing method: (7) After (2) and (5) to (6) are uniformly dissolved, (4) is dissolved and added to (1), and then (3)
Add to thicken. As for the peony extract, 200 g of peony ( Paeoniae Radix ) is dried and crushed to obtain 5
Dip in 1 liter of 0% by volume ethanol solution for 25
The mixture was allowed to stand at 7 ° C for 7 days and then filtered to obtain a filtrate.

[0032]

[Table 4]

[Examples 9 and 10] Oil-in-water emulsion type ointment (1) White petrolatum 25.00 (2) Stearyl alcohol 25.00 (3) Glycerin 12.00 (4) Sodium lauryl sulfate 1.00 (5) Methyl paraoxybenzoate 0.10 (6) Amount based on 100 as the total amount of purified water (7) Anti-inflammatory agent shown in Table 5 0.10 (8) Swallowtail plant extract 2 0.50 Production method: (1 ) To (4), the oil phase components are mixed and heated to uniformly dissolve, and the temperature is adjusted to 75 ° C. On the other hand, the aqueous phase components (5) and (6) are mixed and heated to 75 ° C. The above oil phase component is gradually added to this water phase component while stirring to emulsify, and after cooling, (7) and (8) are added and mixed at 40 ° C.
In addition, kujin extract is kujin ( SophoraeRadix ) 20
0 g was dried, pulverized, immersed in 1 liter of a 50% by volume aqueous ethanol solution, allowed to stand at 25 ° C. for 7 days, and then filtered to obtain a filtrate.

[0034]

[Table 5]

Example 1, Example 4, Example 5 of the present invention
A usage test was conducted on Examples 8 and 9 to evaluate the moisturizing properties, the effect of improving skin aging symptoms, and the effect of improving rough skin. At that time, in the above-mentioned Example, the blended swallow moto plant extract 1 to swallow moto plant extract 3 was used as each extraction solvent, and the swallow moto plant extract 4 was replaced with squalane, and comparative example 1, comparative example 4, and comparative example Example 5, Comparative Example 8,
As Comparative Example 9, a usage test was conducted at the same time.

First, regarding the moisturizing property, for each sample,
Twenty female panelists in their 20s to 50s were used in the blind for 2 weeks to evaluate the moist feeling. The evaluation was carried out in five grades of "very moist", "present", "somewhat", "somewhat", and "not".
The results are shown in Table 6 by the number of panelists given each evaluation.

[0037]

[Table 6]

As is clear from Table 6, in the use groups of each Example in which an extract of the genus Plant of the genus Swallowtail and an anti-inflammatory agent were used in combination, a very high evaluation was obtained regarding the moisturizing property. Good results were obtained as compared with the comparative example use group containing no substance.

Next, for improving the skin aging symptoms, each sample was made to have 20 male and female panelists in their 50s to 60s who were using the same skin blindly for 1 month. The change in state was observed and evaluated. Skin wrinkles, texture,
The three items of the acupuncture were selected, and wrinkles and texture were observed by taking photographs and replicas of the skin surface, and the improvement status was evaluated. The skin elasticity was evaluated by measuring the skin elasticity. The improvement status was evaluated in three stages of “improvement”, “slight improvement” and “no change”, and Table 7 shows the number of panelists who obtained each evaluation.

[0040]

[Table 7]

As is apparent from Table 7, in each of the groups used in Comparative Examples which did not contain the extract of the plant of the genus Swallowtail, wrinkles and the tendency to improve the skin elasticity were observed to some extent. In the group used in the examples in which the anti-inflammatory agent was used in combination, the number of panelists who showed a clear improvement was larger than that in the corresponding group used in the comparative example.

For the improvement of rough skin, 20 female panelists each having rough skin symptoms were used blindly for 1 month for each sample, and the improvement of rough skin condition was evaluated. The evaluation was made in five stages: "improved", "slightly improved", "no change", "slightly worse", and "worse". The results are shown in Table 8 by the number of panelists given each evaluation.

[0043]

[Table 8]

As is clear from Table 8, in the groups used in Examples of the present invention in which the extract of the plant of the genus Swallowtail genus and the anti-inflammatory agent were used in combination, remarkable improvement in rough skin was observed in all cases, and the use test was completed. After that, the condition of the skin was improved to almost good condition. On the other hand, in the comparative example use group, although there was a case in which the skin roughness was considerably improved, the degree thereof was smaller than that of the corresponding example use group.

As described above, in the external preparation for skin of the example of the present invention, as compared with the conventional comparative example, a superior moisturizing effect,
It had the effect of improving skin aging and rough skin.

[0046]

As described in detail above, according to the present invention, it is possible to obtain a skin external preparation having an excellent moisturizing effect and synergistically improving the skin aging symptoms such as wrinkles and the rough skin improving effect. did it.

Front page continuation (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 7/00 A61K 7/00 MN RW 7/48 7/48 31/19 31/19 31/60 31/60 31/704 31/704 45/00 45/00 A61P 17/16 A61P 17/16 43/00 121 43/00 121 F term (reference) 4C083 AA082 AA111 AA112 AB032 AC012 AC022 AC072 AC102 AC122 AC422 AC432 AC442 AC482 AC782 AD092 AD112 AD152 AD512 BB51 CC05 DD23 DD27 DD31 DD33 DD41 EE12 4C084 AA19 MA02 NA05 ZA89 ZC75 4C086 AA01 DA17 EA10 MA02 MA04 NA05 ZA89 ZC75 4C088 AB12 AB32 AB40 AB85 AC01 CA03 NA05 ZA89 ZC75 4C206 AA01 A04 NAA02 A02

Claims (3)

[Claims] 1. A skin external preparation comprising an extract of a plant of the genus Clintonia of the family Uvulariaceae and one or more selected from anti-inflammatory agents. 2. The plant of the genus Clintonia of the family Uvulariaceae is Clintonia borealis or Clintonia udensis, which is one or two species. The external preparation for skin according to claim 1. 3. The anti-inflammatory agent is glycyrrhizic acid, glycyrrhetinic acid, azulene, salicylic acid, allantoin and their derivatives, and their salts, Sikon ( Lithosperm).
i Radix ), carrot ( Ginseng Radix ), peony ( Paeoniae Radix ), button pie ( Moutan Cortex ), and ginseng ( Sophorae Radix ). The external preparation for skin according to claim 1 or 2.